Control system of sample analyzer and sample analyzer

Abstract

The utility model discloses a sample analyzer and a control system thereof. The control system comprises a plurality of first main control boards used for controlling the nucleic acid extraction area; the plurality of second main control boards are used for controlling the reagent configuration area; the plurality of third main control boards are used for controlling the amplification detection area; the first central control board is in communication connection with the plurality of first main control boards and the plurality of second main control boards; the second central control board is in communication connection with the plurality of third main control boards; the upper computer is in communication connection with the first central control board and the second central control board and is used for integrally controlling the nucleic acid extraction area, the reagent configuration module and the amplification detection area; and the optical detection module is used for receiving the operation instruction sent by the second central control board and sending detection image data to the upper computer. On one hand, the two central control boards are adopted as the middle layer to simplify the burden of processing the data volume of multiple modules by the upper computer. And on the other hand, the optical detection module is directly connected to the upper computer, so that the transmission efficiency of the detected image data is improved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and particularly to a control system and a sample analyzer for a sample analyzer. Background Art

[0002] A sample analyzer is an instrument that analyzes a patient's body fluid sample using real-time fluorescence quantitative PCR technology, and has the advantages of high sensitivity, simple instrument equipment, convenient operation, fast analysis speed, etc. At present, it is widely used in the fields of medical, agricultural and animal husbandry, and biological related molecular biological quantitative research.

[0003] With the increase of the control mechanism and functions of the sample analyzer, the requirements for the data transmission volume, real-time performance and system stability of the sample analyzer are getting higher and higher. The centralized control structure of the traditional sample analyzer will not only cause a huge pressure on the network bandwidth, but also increase the data processing burden of the upper computer, thus affecting the performance and response speed of the entire system. Moreover, the traditional centralized control structure also has the problems of low resource retrieval efficiency and inability to meet the needs of massive picture transmission. Utility Model Content

[0004] Based on this, in view of the above problems, it is necessary to provide a control system and a sample analyzer for a sample analyzer that can meet the needs of massive picture processing.

[0005] A control system for a sample analyzer, characterized by comprising:

[0006] A first group of main control boards, used to control the nucleic acid extraction area and collect the status information of the nucleic acid extraction area, and the first group of main control boards includes a plurality of first main control boards;

[0007] A second group of main control boards, used to control the reagent preparation area and collect the status information of the reagent preparation area, and the second group of main control boards includes a plurality of second main control boards;

[0008] A third group of main control boards, used to control the amplification detection area and collect the status information of the amplification detection area, and the third group of main control boards includes a plurality of third main control boards;

[0009] A first central control board, communicatively connected to the first group of main control boards and the second group of main control boards respectively, the first central control board is used to control the nucleic acid extraction area and the reagent preparation area, and collect the status information of the nucleic acid extraction area and the reagent preparation area;

[0010] A second central control board, communicatively connected to the third group of main control boards, the second central control board is used to control the plurality of third main control boards, and collect the status information of the amplification detection area;

[0011] The host computer is communicatively connected to the first central control board and the second central control board respectively, and the host computer is used to overall control the nucleic acid extraction area, the reagent configuration module, and the amplification detection area; and

[0012] The optical detection module is connected to the host computer and the second central control board respectively. The optical detection module is used to receive the operation instructions sent by the second central control board and send the detection image data to the host computer.

[0013] In some embodiments, the first central control board and the host computer are communicatively connected through a network cable;

[0014] The second central control board and the host computer are communicatively connected through a network cable;

[0015] The optical detection module and the host computer are communicatively connected through a network cable;

[0016] The first central control board is connected to the first group of main control boards and the second group of main control boards respectively through a CANBus bus;

[0017] The second central control board is connected to the third group of main control boards through a CANBus bus.

[0018] In some embodiments, the control system of the sample analyzer further includes:

[0019] A network switch is connected to the host computer, the first central control board, and the second central control board respectively. The host computer exchanges data with the first central control board and the second central control board respectively through the network switch.

[0020] In some embodiments, the optical detection module is connected to the host computer through the network switch, and the optical detection module is connected to the second central control board through a power supply and control signal line.

[0021] In some embodiments, the multiple first main control boards include one or more of the following main control boards: the main control board of the waste liquid treatment module in the nucleic acid extraction area, the main control board of the film sealing control module in the nucleic acid extraction area, the main control board of the sample transfer and lid opening control module in the nucleic acid extraction area, and the main control board of the multi-mechanism coordination control module in the nucleic acid extraction area.

[0022] In some embodiments, the multiple second main control boards include one or more of the following main control boards: the main control board of the liquid treatment module in the reagent configuration area, and the main control board of the reagent and sample operation module in the reagent configuration area.

[0023] In some embodiments, the first set of main control boards, the second set of main control boards, and the first central control board are connected using a CANBus bus, and the connection sequence is as follows: the first set of main control boards, the first central control board, and the second set of main control boards.

[0024] In some embodiments, the CANBus node connection sequence of the multiple first main control boards, the multiple second main control boards, and the first central control board is as follows:

[0025] First node: the main control board of the waste liquid treatment module in the nucleic acid extraction area; Second node: the main control board of the film sealing control module in the nucleic acid extraction area; Third node: the main control board of the sample transfer and lid opening control module in the nucleic acid extraction area; Fourth node: the main control board of the multi-mechanism coordination control module in the nucleic acid extraction area; Fifth node: the first central control board; Sixth node: the main control board of the liquid processing module in the reagent preparation area; Seventh node: the main control board of the reagent and sample operation module in the reagent preparation area.

[0026] In some embodiments, the multiple third main control boards include one or more of the following main control boards: the main control board of the PCR temperature control module in the amplification and detection area, and the main control board of the amplification plate transportation module in the amplification and detection area.

[0027] In some embodiments, the multiple third main control boards are connected to the second central control board using a CANBus bus, and their CANBus node connection sequence is as follows:

[0028] Eighth node: the second central control board; Ninth node: the main control board of the PCR temperature control module in the amplification and detection area; Tenth node: the main control board of the amplification plate transportation module in the amplification and detection area.

[0029] In some embodiments, the first central control board includes a network data interface and a CANBus data interface. The network data interface of the first central control board is converted into standard network data through a network port communication driving circuit and communicates with the host computer through a network cable interface. The CANBus data interface of the first central control board is converted into bus data through a CANBus bus node driving circuit and communicates with the bus interfaces of the first set of main control boards and the second set of main control boards through a bus interface;

[0030] The second central control board includes a network data interface and a CANBus data interface. The network data interface of the second central control board is converted into standard network data through a network port communication driving circuit and communicates with the host computer through a network cable interface. The CANBus data interface of the second central control board is converted into bus data through a CANBus bus node driving circuit and communicates with the bus interface of the third set of main control boards through a bus interface;

[0031] The optical detection module includes a network data interface. The network data interface of the optical detection module is converted into standard network data through a network port communication drive circuit and communicates with the host computer through a network cable interface.

[0032] A sample analyzer includes a nucleic acid extraction area, a reagent configuration area, an amplification detection area, a host computer, and an optical detection module. The nucleic acid extraction area includes a first group of main control boards, and the first group of main control boards includes multiple first main control boards; the reagent configuration area includes a second group of main control boards, and the second group of main control boards includes multiple second main control boards; the amplification detection area includes a third group of main control boards, and the third group of main control boards includes multiple third main control boards;

[0033] The nucleic acid extraction area further includes a first central control board. The first central control board is respectively communicatively connected to the first group of main control boards and the second group of main control boards. The first central control board is used to control the nucleic acid extraction area and the reagent configuration area, and collect the status information of the nucleic acid extraction area and the reagent configuration area;

[0034] The amplification detection area further includes a second central control board. The second central control board is communicatively connected to the third group of main control boards. The second central control board is used to control the multiple third main control boards and collect the status information of the amplification detection area;

[0035] The host computer is respectively communicatively connected to the first central control board and the second central control board. The host computer is used to perform overall control on the nucleic acid extraction area, the reagent configuration module, and the amplification detection area;

[0036] The optical detection module is respectively connected to the host computer and the second central control board. The optical detection module is used to receive the operation instructions sent by the second central control board and send detection image data to the host computer.

[0037] In the control system of the above sample analyzer, a three - level electronic hardware architecture of "host computer + 2 central control boards + multiple main control boards" is used to achieve hierarchical control of each module of the sample analyzer. The host computer is responsible for the management and monitoring of the overall system, provides a user interface, simplifies operation and data management, and improves the reliability of the system. As the middle layer, the central control board coordinates the work of each main control board, reduces the complexity of directly controlling multiple modules by the host computer, increases the number of system control modules, and improves the stability of the system. Specifically, two central control boards are introduced as the middle layer to simplify the burden of the host computer in processing the data volume of multiple modules. The first central control board controls the nucleic acid extraction module and the reagent preparation module. By setting the modules with more interactions under the first central control board, compared with cross - board communication, this significantly reduces the signal transmission time and improves the data transmission and processing efficiency. The second central control board controls multiple third main control boards to collect the status information of the amplification and detection area, thus reducing the resource occupation of the host computer. The main control board directly controls specific modules to achieve fine control and data acquisition, shares the workload, and reduces the impact of single - point failures. Therefore, the control system of the above sample analyzer can achieve the efficient operation of the sample analyzer, and on the basis of realizing the control of a large number of modules, improve the overall machine transmission efficiency. In addition, in the control system of the above sample analyzer, due to the large number of image processing requirements of the sample analyzer, in order to improve the image processing efficiency and quality, the optical detection module is directly connected to the host computer, and the host computer directly controls it to improve the operation efficiency. This method can achieve high - speed transmission of photo data, reduce intermediate transmission nodes, and reduce the delay and bandwidth occupation in the data transmission process. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 FIG. is a schematic structural diagram of the control system of the sample analyzer provided by one embodiment of the present application;

[0039] Figure 2 is Figure 1 a schematic structural diagram of the connection between the host computer in and the first central control board, the second central control board, and the optical detection module through a network interface;

[0040] Figure 3 is Figure 1 a schematic diagram of the connection of the CANBus bus nodes between the first central control board and the first group of main control boards and the second group of main control boards in;

[0041] Figure 4 is Figure 1 a schematic diagram of the connection of the CANBus bus nodes between the second central control board and the third group of main control boards in;

[0042] Figure 5 is a schematic diagram of the circuit connection of the main control chip of one of the main control boards;

[0043] Figure 6Schematic diagram of the connection of the network port communication drive circuit for one of the central control boards;

[0044] Figure 7 Schematic diagram of the connection of the CANBus bus node drive circuit;

[0045] Figure 8 Schematic diagram of the structure of the power supply and external trigger signal line connection socket for the optical detection module. Detailed implementation manners

[0046] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed implementation manners of the present application in conjunction with the accompanying drawings. Many specific details are set forth in the following description to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0047] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "outer circumference", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present application.

[0048] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present application, "a plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0049] In the present application, unless otherwise clearly defined and limited, the terms "install", "connect", "connection", "fix", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0050] In this application, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply indicates that the first feature has a lower horizontal height than the second feature.

[0051] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are only for illustrative purposes and do not represent the only implementation.

[0052] Please refer to Figures 1 to 2 , a control system 100 of a sample analyzer is provided in one embodiment of the present application. The control system 100 of the sample analyzer includes a first group of main control boards 110, a second group of main control boards 120, a third group of main control boards 130, a first central control board 140, a second central control board 150, a host computer 160, and an optical detection module 180.

[0053] The first group of main control boards 110 is used to control the nucleic acid extraction area 10 and collect the status information of the nucleic acid extraction area 10. The first group of main control boards 110 includes a plurality of first main control boards. In this embodiment, the nucleic acid extraction area 10 includes mechanisms such as a waste liquid treatment module, a film sealing control module, a sample transfer and lid opening control module, and a multi-mechanism coordination control module. The first group of main control boards 110 is used to control the mechanisms in the nucleic acid extraction area 10 to perform steps such as sample addition, nucleic acid extraction, and nucleic acid addition. At the same time, the first group of main control boards 110 is also used to collect the status information of the mechanisms in the nucleic acid extraction area 10.

[0054] The second group of main control boards 120 is used to control the reagent preparation area 20 and collect the status information of the reagent preparation area 20. The second group of main control boards 120 includes a plurality of second main control boards. In this embodiment, the reagent preparation area 20 includes mechanisms such as a liquid treatment module, a reagent and sample operation module. The second group of main control boards 120 is used to control the mechanisms in the reagent preparation area 20 to perform steps such as reagent preparation and amplification reagent preparation. At the same time, the second group of main control boards 120 is also used to collect the status information of the mechanisms in the reagent preparation area 20.

[0055] The third set of main control boards 130 is used to control the amplification detection area 30 and collect the status information of the amplification detection area 30. The third set of main control boards 130 includes a plurality of third main control boards. In this embodiment, the amplification detection area 30 includes mechanisms such as a PCR temperature control module and an amplification plate gripper. The third set of main control boards 130 is used to control the mechanisms in the amplification detection area 30 to perform steps such as PCR amplification. At the same time, the third set of main control boards 130 is also used to collect the status information of the mechanisms in the amplification detection area 30.

[0056] The first central control board 140 is respectively communicatively connected to the first set of main control boards 110 and the second set of main control boards 120. The first central control board 140 is used to control the nucleic acid extraction area 10 and the reagent configuration area 20, and collect the status information of the nucleic acid extraction area 10 and the reagent configuration area 20. In this embodiment, considering that there are many interaction actions between the nucleic acid extraction area 10 and the reagent configuration area 20, using one central control board to control the nucleic acid extraction area 10 and the reagent configuration area 20 can improve the interaction efficiency between the nucleic acid extraction area 10 and the reagent configuration area 20. In fact, in existing sample analyzers, the nucleic acid extraction area 10 and the reagent configuration area 20 are generally independently controlled. However, due to the large number of interaction actions between the nucleic acid extraction area 10 and the reagent configuration area 20, if these two modules are independently controlled respectively, it will affect the interaction efficiency between the nucleic acid extraction area 10 and the reagent configuration area 20. In the control system 100 of the sample analyzer of the present application, the modules with more interactions are set under the first central control board. Compared with cross-board communication, this significantly reduces the signal transmission time and improves the data transmission and processing efficiency.

[0057] The second central control board 150 is communicatively connected to the third set of main control boards 130. The second central control board 150 is used to control the plurality of third main control boards and collect the status information of the amplification detection area 30. In this embodiment, since the amplification detection area 30 is relatively independent, one central control board can be used to independently control the third set of main control boards 130, thereby avoiding too long resource retrieval time in the amplification detection area 30. That is, the second central control board 150 can control a plurality of third main control boards to collect the status information of the amplification detection area 30, thereby reducing the resource occupancy of the host computer.

[0058] The host computer 160 is respectively communicatively connected to the first central control board 140 and the second central control board 150. The host computer 160 is used to perform overall control of the nucleic acid extraction area 10, the reagent configuration area 20, and the amplification detection area 30.

[0059] The optical detection module 180 is respectively connected to the host computer 160 and the second central control board 150. The optical detection module 180 is configured to receive the operation instructions sent by the second central control board 150 and send the detection image data to the host computer 160.

[0060] In the control system 100 of the sample analyzer provided in the above embodiments, a three - level electronic hardware architecture of "host computer + 2 central control boards + multiple main control boards" can be used to achieve hierarchical control of each module of the sample analyzer. The host computer is responsible for the management and monitoring of the overall system, provides a user interface, simplifies operation and data management, and improves the reliability of the system. The central control board, as the middle layer, coordinates the work of each main control board, reduces the complexity of directly controlling multiple modules by the host computer, increases the number of system control modules, and improves the stability of the system. Specifically, two central control boards are introduced as the middle layer to simplify the burden of the host computer in processing the data volume of multiple modules. The first central control board controls the nucleic acid extraction module and the reagent preparation module. By setting the modules with more interactions under the first central control board, compared with cross - board communication, this significantly reduces the signal transmission time and improves the data transmission and processing efficiency. The second central control board controls multiple third main control boards to collect the status information of the amplification detection area, thereby reducing the resource occupancy of the host computer. The main control board directly controls specific modules to achieve fine control and data acquisition, shares the workload, and reduces the impact of single - point failures. Therefore, the control system of the above sample analyzer can achieve the efficient operation of the sample analyzer and improve the overall machine transmission efficiency on the basis of realizing the control of a large number of modules.

[0061] In addition, in the control system of the above sample analyzer, since the sample analyzer has a large number of picture - processing requirements, to improve the picture - processing efficiency and quality, the optical detection module is separately connected to the host computer, and the host computer directly controls it to improve the operation efficiency.

[0062] On the other hand, in the control system 100 of the above sample analyzer, the three modules of the nucleic acid extraction area 10, the reagent configuration area 20, and the amplification detection area 30 are controlled by two central control boards, which can also achieve load balancing and efficient management. Specifically, the design of two central control boards can effectively share the workload of the system, avoid overloading of a single central control board, and improve the efficiency of the overall system. On the other hand, the design of the central control board also enhances the scalability of the system. If new functional areas need to be added in the future, only new central control boards need to be added without redesigning the entire system architecture, so that the control system of the above sample analyzer has good scalability.

[0063] In some embodiments, the first central control board 140 is communicatively connected to the host computer 160 through a network cable.

[0064] The second central control board 150 is communicatively connected to the host computer 160 via a network cable.

[0065] The optical detection module 180 is communicatively connected to the host computer 160 via a network cable.

[0066] The first central control board 140 is respectively connected to the first group of main control boards 110 and the second group of main control boards 120 via a CANBus bus.

[0067] The second central control board 150 is connected to the third group of main control boards 130 via a CANBus bus.

[0068] In the control system of the above sample analyzer, the host computer and the central control board are connected via a network cable, and the central control board and the main control board communicate via a CANBus bus. Compared with the serial communication method, network communication can achieve the rapid transmission and processing of a large amount of data, greatly improving the communication efficiency and communication effect. At the same time, network communication adopts protocols such as TCP / IP. By splitting the data into data packets and performing checksum and confirmation on them, the reliability and integrity of data transmission are guaranteed. Therefore, the host computer and the central control board adopting network communication can achieve reliable and high-speed transmission of big data. And the central control board and the main control board communicate via a CANBus bus, which can achieve simple wiring and reliable real-time data transmission.

[0069] It should be noted that the CANBus (Controller Area Network Bus) is a serial communication network that can effectively support distributed control and real-time control. It mainly consists of a CAN controller, a CAN transceiver, a CAN data bus, and a CAN data transfer terminal. The data transmission in the CANBus system is broadcast to all nodes in the network in the form of messages. Each node can send data at any time. When multiple nodes send data simultaneously, the node with a lower priority will actively withdraw from sending. The CANBus has the advantages of high data transmission speed, saving wiring harnesses, optimizing the wiring method, and the failure of one control unit does not affect the data transmission of other control units. In the control system of the above sample analyzer, by taking the first central control board 140 as one of the nodes of the CANBus, and taking the main control boards of mechanisms such as the waste liquid treatment module, the film sealing control module, the sample transfer and lid opening control module, the multi-mechanism coordination control module, the liquid treatment module, and the reagent and sample operation module as the other multiple nodes of the CANBus respectively, the above setting method can make the wiring between the first central control board 140 and multiple first main control boards and multiple second main control boards simple, with real-time data transmission and fast transmission speed. Similarly, by taking the second central control board 150 as one of the nodes of the CANBus, and taking mechanisms such as the PCR temperature control module and the amplification plate gripper as the other multiple nodes of the CANBus respectively, the above setting method can also make the wiring between the second central control board 150 and multiple third main control boards simple, with real-time data transmission and fast transmission speed.

[0070] In one embodiment, the control system 100 of the sample analyzer further includes an Ethernet switch 170.

[0071] The network port switch 170 is respectively connected to the host computer 160, the first central control board 140, and the second central control board 150. The data interaction between the host computer 160 and the first central control board 140 and the second central control board 150 is carried out through the network port switch 170. In this embodiment, the host computer 160 and the first central control board 140 and the second central control board 150 perform network communication through the network port switch 170, so as to achieve reliable transmission of big data. In the specific control process, the user interacts through the software in the host computer 160 to implement operations such as inputting control commands. Then, the host computer 160 issues relevant control commands to the first central control board 140 and / or the second central control board 150 through the network port. Then, the first central control board 140 and / or the second central control board 150 forward the corresponding control commands to the corresponding main control boards by parsing the parameters. It can be seen that in the control system 100 of the above sample analyzer, the division of labor among the hardware boards at all levels is clear. Even if some hardware fails, the above hardware architecture helps to quickly locate the fault source and simplifies the fault diagnosis and maintenance work. In fact, by controlling multiple first main control boards and multiple second main control boards through the first central control board 140, when one of the first main control boards or the second main control boards fails, replacing the faulty main control board can solve the problem without replacing the entire system. Similarly, by controlling multiple third main control boards through the second central control board 150, when one of the first main control boards or the third main control boards fails, replacing the faulty main control board can solve the problem. That is, in the control system 100 of the sample analyzer, replacing a single faulty board is more convenient than replacing the entire system, thus making the maintenance cost of the control system 100 of the sample analyzer relatively low.

[0072] In this embodiment, the optical detection module 180 is connected to the host computer 160 via a network cable. In this embodiment, the optical detection module 180 is connected to the network port switch 170 via a network cable, and then connected to the host computer 160 via the network port switch 170, so as to realize network communication with the host computer 160. The optical detection module 180 is connected to the second central control board 150 via a power supply and control signal line. Specifically, the operation instructions of the second central control board 150 include turning on or off the optical detection module 180, or controlling the shutter of the optical detection module 180 to take pictures. Since the above operation instructions are relatively simple and the amount of data transmitted is small, it can be controlled by the second central control board 150, and the corresponding data can also be transmitted via the power supply and control signal line. Regarding the detection image data captured by the optical detection module 180 and the processing of the corresponding detection image data, etc., since the amount of the corresponding detection image data is relatively large, it needs to be transmitted to the host computer 160 via the network port switch 170, so as to realize high-speed data transmission. In this embodiment, the optical detection module 180 is directly connected to the network port switch 170, and the host computer 160 processes the photo data. Since the optical detection module 180 is directly connected to the host computer 160 via the network port switch 170, this method can realize high-speed transmission of photo data, reduce intermediate transmission nodes, and reduce the delay and bandwidth occupation during data transmission. At the same time, the optical detection module 180 is directly connected to the host computer 160 via the network port switch 170, this method reduces the intermediate links in the system architecture, simplifies the system design and implementation, and reduces the hardware cost and system complexity.

[0073] Please also refer to Figure 3, in one embodiment, the multiple first main control boards include one or more of the following main control boards: the main control board 111 of the waste liquid treatment module in the nucleic acid extraction area, the main control board 112 of the film sealing control module in the nucleic acid extraction area, the main control board 113 of the sample transfer and lid opening control module in the nucleic acid extraction area, and the main control board 114 of the multi-mechanism coordination control module in the nucleic acid extraction area. The main control board 111 of the waste liquid treatment module in the nucleic acid extraction area is used to control the waste liquid station and collect the status information of the waste liquid station. As needed, the main control board 111 of the waste liquid treatment module in the nucleic acid extraction area can also be one or more. The main control board 112 of the film sealing control module in the nucleic acid extraction area is used to control the film sealing mechanism and collect the status information of the film sealing mechanism. As needed, the main control board 112 of the film sealing control module in the nucleic acid extraction area can also be one or more. The main control board 113 of the sample transfer and lid opening control module in the nucleic acid extraction area is used to control the sample transfer mechanism and the lid opening mechanism and collect the status information of the sample transfer mechanism and the lid opening mechanism. As needed, the main control board 113 of the sample transfer and lid opening control module in the nucleic acid extraction area can also be one or more. The main control board 114 of the multi-mechanism coordination control module in the nucleic acid extraction area is used to control multiple mechanisms in the extraction area and collect the status information of multiple mechanisms in the extraction area. As needed, the main control board 114 of the multi-mechanism coordination control module in the nucleic acid extraction area can also be one or more.

[0074] The multiple second main control boards include one or more of the following main control boards: the main control board 121 of the liquid treatment module in the reagent preparation area, and the main control board 122 of the reagent and sample operation module in the reagent preparation area. The main control board 121 of the liquid treatment module in the reagent preparation area is used to control the liquid treatment mechanism and collect the status information of the liquid treatment mechanism. As needed, the main control board 121 of the liquid treatment module in the reagent preparation area can also be one or more. The main control board 122 of the reagent and sample operation module in the reagent preparation area is used to control the reagent and sample operation mechanism and collect the status information of the reagent and sample operation mechanism. As needed, the main control board 122 of the reagent and sample operation module in the reagent preparation area can also be one or more.

[0075] The first group of main control boards 110, the second group of main control boards 120, and the first central control board 140 are connected using the CANBus bus. The connection sequence is as follows: the first group of main control boards 110, the first central control board 140, and the second group of main control boards 120.

[0076] Specifically, the CANBus node connection sequence of the multiple first main control boards, the multiple second main control boards, and the first central control board 140 is as follows:

[0077] First node: the main control board 111 of the waste liquid treatment module in the nucleic acid extraction area.

[0078] The second node: the main control board 112 of the nucleic acid extraction area film sealing control module.

[0079] The third node: the main control board 113 of the nucleic acid extraction area sample transfer and lid opening control module.

[0080] The fourth node: the main control board 114 of the nucleic acid extraction area multi-institution coordination control module.

[0081] The fifth node: the first central control board 140.

[0082] The sixth node: the main control board 121 of the reagent preparation area liquid processing module.

[0083] The seventh node: the main control board 122 of the reagent preparation area reagent and sample operation module.

[0084] In this embodiment, by setting the CANBus node position of the first central control board 140 between the CANBus nodes of the first group of main control boards 110 and the second group of main control boards 120, the first central control board 140 can more quickly control the equipment in the nucleic acid extraction area 10 and the reagent preparation area 20.

[0085] Please refer to Figure 4 , in one of the embodiments, the multiple third main control boards include one or more of the following main control boards: the main control board 131 of the PCR temperature control module in the amplification and detection area, the main control board 132 of the amplification plate transportation module in the amplification and detection area. The main control board 131 of the PCR temperature control module in the amplification and detection area is used to control the PCR temperature control module and collect the status information of the PCR temperature control module. As needed, the main control board 131 of the PCR temperature control module in the amplification and detection area can also be one or more. The main control board 132 of the amplification plate transportation module in the amplification and detection area is used to control the amplification plate gripper and collect the status information of the amplification plate gripper. As needed, the main control board 132 of the amplification plate transportation module in the amplification and detection area can also be one or more.

[0086] Among them, the multiple third main control boards are connected to the second central control board 150 by a CANBus bus, and the connection order of its CANBus nodes is:

[0087] The eighth node: the second central control board 150.

[0088] The ninth node: the main control board 131 of the PCR temperature control module in the amplification and detection area.

[0089] The tenth node: the main control board 132 of the amplification plate transportation module in the amplification and detection area.

[0090] In this embodiment, the connection sequence of the CANBus nodes of the second central control board 150 and the multiple third main control boards is set as follows: the second central control board 150, the PCR temperature control module main control board 131 in the amplification detection area, and the amplification plate transportation module main control board 132 in the amplification detection area. The second central control board 150 can effectively control the operations in the amplification detection area 30.

[0091] In one embodiment, the first central control board 140 includes a network data interface and a CANBus data interface. The network data interface of the first central control board 140 is converted into standard network data through a network port communication driving circuit and communicates with the host computer through a network cable interface. The CANBus data interface of the first central control board 140 is converted into bus data through a CANBus bus node driving circuit and communicates with the bus interfaces of the first group of main control boards 110 and the second group of main control boards 120 through a bus interface.

[0092] The second central control board 150 includes a network data interface and a CANBus data interface. The network data interface of the second central control board 150 is converted into standard network data through a network port communication driving circuit and communicates with the host computer 160 through a network cable interface. The CANBus data interface of the second central control board 150 is converted into bus data through a CANBus bus node driving circuit and communicates with the bus interfaces of the third group of main control boards through a bus interface.

[0093] The optical detection module 180 includes a network data interface. The network data interface of the optical detection module 180 is converted into standard network data through a network port communication driving circuit and communicates with the host computer 160 through a network cable interface.

[0094] Specifically, Figures 5 to 8 Specific structure diagrams of the central control board, the network port communication driving circuit, the CANBus bus node driving circuit, the power supply for the optical detection module, and the external trigger signal line connection socket are respectively disclosed. Figure 5 It is the chip circuit diagram of the second central control board. As Figure 5 shown, the first chip therein includes a CANBus bus data interface, a network data interface, and an optical detection module data interface.

[0095] The CANBus bus data interface is used to transmit CANBus bus data CAN2_RX_IO and CAN2_TX_IO.

[0096] The network data interface is used to transmit network data: PA0_ETH_RESETH_IO, PA1_ETH_RMII_REF_CLK_IO, PA2_ETH_MDIO_IO, PA3_ETH_INT_IO, DEBUG_LED2, PA7_ETH_RMII_CRS_DV_IO, PB11_ETH_RMII_TX_EN_IO, PB12_ETH_RMII_TXD0_IO, PB13_ETH_RMII_TXD1_IO, PC1_ETH_MDC_IO, PC4_ETH_RMII_RXD0_IO, PC5_ETH_RMII_RXD1_IO.

[0097] The data interface of the optical detection module is used to transmit the power signal and control signal of the optical detection module: OPTICAL_MODULEX_CAMERA_IN, OPTICAL_MODULEX_CAMERA_OUT1.

[0098] Figure 6 It is the network communication driver circuit diagram of the second central control board. As Figure 6 shown, the second chip therein includes a network data interface. The network data interface is used to transmit network data: PA0_ETH_RESETH_IO, PA1_ETH_RMII_REF_CLK_IO, PA2_ETH_MDIO_IO, PA3_ETH_INT_IO, DEBUG_LED2, PA7_ETH_RMII_CRS_DV_IO, PB11_ETH_RMII_TX_EN_IO, PB12_ETH_RMII_TXD0_IO, PB13_ETH_RMII_TXD1_IO, PC1_ETH_MDC_IO, PC4_ETH_RMII_RXD0_IO, PC5_ETH_RMII_RXD1_IO. In this embodiment, the network data interface of the second chip corresponds to the network data interface of the first chip. During actual use, the second chip receives the network data transmitted by the first chip and transmits the corresponding network data to the outside through the RJ45 interface. Or, the second chip receives the network data transmitted from the outside through the RJ45 interface and transmits the network data back to the first chip.

[0099] Figure 7 It is the specific structure of the CANBus bus node driver circuit. As Figure 7As shown, the drive circuit uses the TJA1051T / 3 (SO8) (NXP) CAN transceiver chip to build the CANBus bus communication circuit. In this drive circuit, the CAN transceiver chip has a CANBus bus data interface. The CANBus bus data interface is used to transmit CANBus bus data CAN2_RX_IO and CAN2_TX_IO. Specifically, the CANBus bus data interface of the CAN transceiver chip corresponds to the CANBus bus data interface of the first chip. The CAN transceiver chip receives the data transmitted by the CANBus bus data interface of the first chip and converts it into bus data to communicate with the outside world through the CAN2L and CAN2H interfaces. In this embodiment, the TJA1051T / 3 is a high-performance CAN transceiver that can ensure the reliability and stability of data transmission. To achieve the best communication effect, the communication connection line selects a combination of shield + twisted pair. The shielding layer can effectively prevent external electromagnetic interference, while the twisted pair improves the signal integrity by canceling out interference signals with each other.

[0100] Figure 8 Schematic diagram of the structure of the power supply for the optical detection module and the external trigger signal line connection socket. As Figure 8 shown, the connection socket includes an optical detection module data interface. The optical detection module data interface is used to transmit the power supply signal and control signal of the optical detection module: OPTICAL_MODULEX_CAMERA_IN, OPTICAL_MODULEX_CAMERA_OUT1. Specifically, the optical detection module data interface of the connection socket corresponds to the optical detection module data interface of the first chip. The connection socket is used to communicate the optical detection module data of the first chip with the outside world.

[0101] In this embodiment, the host computer 160 performs network communication with the optical detection module 180 through the network switch 170. Specifically, the optical detection module 180 can use the Gige interface to communicate with the host computer 160 to ensure the stability and high speed of data transmission. In addition, the optical detection module 180 not only provides power for the camera but also provides an external trigger signal for the camera to take pictures to ensure the synchronization and accuracy of taking pictures. In this embodiment, the external trigger signals for power supply and taking pictures are provided by the second central control board 150 and transmitted to the optical detection module 180 through the power supply and control signal lines. Then, the main control board of the optical detection module 180 sends the corresponding power supply signal and the external trigger signal for taking pictures to the corresponding camera, thereby realizing the function of taking pictures. The taken pictures will be transmitted to the host computer 160 through the network switch 170, and the host computer 160 obtains the picture data and analyzes the picture data.

[0102] Understandably, the chip circuit diagrams, network port communication driver circuit diagrams, and CANBus bus node driver circuits of the first central control board 140 and the second central control board 150 are similar. Details are not described herein again. In the specific implementation process, since the first central control board 140 does not need to control the optical detection module 180, the chip circuit diagram of the first central control board 140 may not include the data interface of the optical detection module.

[0103] Another embodiment of the present application further provides a sample analyzer, including a nucleic acid extraction area 10, a reagent preparation area 20, an amplification detection area 30, a host computer 160, and an optical detection module 180. The nucleic acid extraction area 10 includes a first group of main control boards 110, and the first group of main control boards 110 includes multiple first main control boards. The reagent preparation area 20 includes a second group of main control boards 120, and the second group of main control boards includes multiple second main control boards. The amplification detection area 30 includes a third group of main control boards 130, and the third group of main control boards includes multiple third main control boards.

[0104] The nucleic acid extraction area 10 further includes a first central control board 140. The first central control board 140 is connected to the first group of main control boards 110 and the second group of main control boards 120 through a CANBus bus. The first central control board 140 is used for information interaction between the nucleic acid extraction area 10 and the reagent preparation area 20.

[0105] The amplification detection area 30 further includes a second central control board 150. The second central control board 150 is connected to the third group of main control boards 130 through a CANBus bus, and the second central control board 150 is used for information interaction between the multiple third main control boards.

[0106] The host computer 160 is connected to the first central control board 140 and the second central control board 150 through a network cable. The host computer 160 is used for overall control of the nucleic acid extraction area 10, the reagent preparation area 20, and the amplification detection area 30.

[0107] The optical detection module 180 is respectively connected to the host computer 160 and the second central control board 150. The optical detection module 180 is used to receive the operation instructions sent by the second central control board 150 and send the detection image data to the host computer 160.

[0108] In the above sample analyzer, a three - level electronic hardware architecture of "host computer + 2 central control boards + multiple main control boards" can be used to achieve hierarchical control of each module of the sample analyzer. The host computer is responsible for the management and monitoring of the overall system, provides a user interface, simplifies operation and data management, and improves the reliability of the system. As the middle layer, the central control board coordinates the work of each main control board, reduces the complexity of directly controlling multiple modules by the host computer, increases the number of system control modules, and improves the stability of the system. Specifically, two central control boards are introduced as the middle layer to simplify the burden of the host computer in processing the data volume of multiple modules. The first central control board controls the nucleic acid extraction module and the reagent preparation module. By setting the modules with more interactions under the first central control board, compared with cross - board communication, this significantly reduces the signal transmission time and improves the data transmission and processing efficiency. The second central control board controls multiple third main control boards to collect the status information of the amplification detection area, thus reducing the resource occupancy of the host computer. The main control board directly controls specific modules, realizes fine control and data acquisition, shares the workload, and reduces the impact of single - point failures. Therefore, the control system of the above sample analyzer can achieve the efficient operation of the sample analyzer, and on the basis of realizing the control of a large number of modules, improve the transmission efficiency of the whole machine. In addition, in the above sample analyzer, due to the large number of image processing requirements of the sample analyzer, to improve the image processing efficiency and quality, the optical detection module is directly connected to the host computer, and the host computer directly controls it to improve the operation efficiency. In one of the embodiments, the optical detection module is directly connected to the network port switch, and the host computer processes the photo data. Since the optical detection module is directly connected to the host computer through the network port switch, this method can achieve high - speed transmission of photo data, reduce the intermediate transmission nodes, and reduce the delay and bandwidth occupancy during the data transmission process. At the same time, since the optical detection module is directly connected to the host computer through the network port switch, this method reduces the intermediate links in the system architecture, simplifies the system design and implementation, and reduces the hardware cost and system complexity.

[0109] The technical features of the above - described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above - described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0110] The above - described embodiments only represent several implementation manners of the present application. Their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A control system for a sample analyzer, characterized in that, Including: The first group of main control boards, which are used to control the nucleic acid extraction area and collect the status information of the nucleic acid extraction area. The first group of main control boards includes multiple first main control boards; The second group of main control boards, which are used to control the reagent preparation area and collect the status information of the reagent preparation area. The second group of main control boards includes multiple second main control boards; The third group of main control boards, which are used to control the amplification detection area and collect the status information of the amplification detection area. The third group of main control boards includes multiple third main control boards; The first central control board is communicatively connected to the first group of main control boards and the second group of main control boards respectively. The first central control board is used to control the nucleic acid extraction area and the reagent preparation area, and collect the status information of the nucleic acid extraction area and the reagent preparation area; The second central control board is communicatively connected to the third group of main control boards. The second central control board is used to control the multiple third main control boards and collect the status information of the amplification detection area; The host computer is communicatively connected to the first central control board and the second central control board respectively. The host computer is used to perform overall control on the nucleic acid extraction area, the reagent preparation area and the amplification detection area; And The optical detection module is connected to the host computer and the second central control board respectively. The optical detection module is used to receive the operation instructions sent by the second central control board and send the detection image data to the host computer.

2. The control system of the sample analyzer according to claim 1, wherein The first central control board is communicatively connected to the host computer through a network cable; The second central control board is communicatively connected to the host computer through a network cable; The optical detection module is communicatively connected to the host computer through a network cable; The first central control board is connected to the first group of main control boards and the second group of main control boards respectively through a CANBus bus; The second central control board is connected to the third group of main control boards through a CANBus bus.

3. The control system of the sample analyzer according to claim 2, characterized in that, It further includes: The network port switch is connected to the host computer, the first central control board and the second central control board respectively. The host computer performs data interaction with the first central control board and the second central control board respectively through the network port switch.

4. The control system of the sample analyzer according to claim 3, characterized in that, The optical detection module is connected to the host computer through the network port switch, and the optical detection module is connected to the second central control board through a power supply and a control signal line.

5. The control system of the sample analyzer according to claim 2, wherein The multiple first main control boards include one or more of the following main control boards: the main control board of the waste liquid treatment module in the nucleic acid extraction area, the main control board of the film sealing control module in the nucleic acid extraction area, the main control board of the sample transfer and cover opening control module in the nucleic acid extraction area, and the main control board of the multi-mechanism coordination control module in the nucleic acid extraction area.

6. The control system of the sample analyzer according to claim 5, characterized in that, The multiple second main control boards include one or more of the following main control boards: the main control board of the liquid treatment module in the reagent preparation area, the main control board of the reagent and sample operation module in the reagent preparation area.

7. The control system of the sample analyzer according to claim 6, characterized in that, The first group of main control boards, the second group of main control boards and the first central control board are connected by a CANBus bus, and the connection sequence is: the first group of main control boards, the first central control board, the second group of main control boards.

8. The control system of the sample analyzer according to claim 7, wherein The connection order of the CANBus bus nodes of the multiple first main control boards, the multiple second main control boards, and the first central control board is as follows: First node: the main control board of the waste liquid treatment module in the nucleic acid extraction area; Second node: the main control board of the film sealing control module in the nucleic acid extraction area; Third node: the main control board of the sample transfer and lid opening control module in the nucleic acid extraction area; Fourth node: the main control board of the multi-mechanism coordination control module in the nucleic acid extraction area; Fifth node: the first central control board; Sixth node: the main control board of the liquid treatment module in the reagent preparation area; Seventh node: the main control board of the reagent and sample operation module in the reagent preparation area.

9. The control system of the sample analyzer according to any one of claims 2-8, characterized in that, The multiple third main control boards include one or more of the following main control boards: the main control board of the PCR temperature control module in the amplification and detection area, and the main control board of the amplification plate transportation module in the amplification and detection area.

10. The control system of the sample analyzer according to claim 9, characterized in that, The multiple third main control boards are connected to the second central control board by using a CANBus bus, and the connection order of its CANBus nodes is as follows: Eighth node: the second central control board; Ninth node: the main control board of the PCR temperature control module in the amplification and detection area; Tenth node: the main control board of the amplification plate transportation module in the amplification and detection area.

11. The control system of the sample analyzer according to any one of claims 2-8, 10, wherein The first central control board includes a network data interface and a CANBus data interface. The network data interface of the first central control board is converted into standard network data through a network port communication driving circuit, and communicates with the upper computer through a network cable interface. The CANBus data interface of the first central control board is converted into bus data through a CANBus bus node driving circuit, and communicates with the bus interfaces of the first group of main control boards and the second group of main control boards through a bus interface; The second central control board includes a network data interface and a CANBus data interface. The network data interface of the second central control board is converted into standard network data through a network port communication driving circuit, and communicates with the upper computer through a network cable interface. The CANBus data interface of the second central control board is converted into bus data through a CANBus bus node driving circuit, and communicates with the bus interface of the third group of main control boards through a bus interface; The optical detection module includes a network data interface. The network data interface of the optical detection module is converted into standard network data through a network port communication driving circuit, and communicates with the upper computer through a network cable interface.

12. A sample analyzer, characterized in that, It includes a nucleic acid extraction area, a reagent preparation area, an amplification and detection area, an upper computer, and an optical detection module. The nucleic acid extraction area includes a first group of main control boards, and the first group of main control boards includes multiple first main control boards; the reagent preparation area includes a second group of main control boards, and the second group of main control boards includes multiple second main control boards; the amplification and detection area includes a third group of main control boards, and the third group of main control boards includes multiple third main control boards; The nucleic acid extraction area further includes a first central control board, which is communicatively connected to the first group of main control boards and the second group of main control boards respectively. The first central control board is used to control the nucleic acid extraction area and the reagent configuration area, and collect the status information of the nucleic acid extraction area and the reagent configuration area; The amplification and detection area further includes a second central control board, which is communicatively connected to the third group of main control boards. The second central control board is used to control the multiple third main control boards and collect the status information of the amplification and detection area; The host computer is communicatively connected to the first central control board and the second central control board respectively. The host computer is used to overall control the nucleic acid extraction area, the reagent configuration module and the amplification and detection area; The optical detection module is connected to the host computer and the second central control board respectively. The optical detection module is used to receive the operation instructions sent by the second central control board and send the detection image data to the host computer.

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