Tissue dissociation device

Abstract

The utility model provides a tissue dissociation device. The tissue dissociation device comprises a dissociation assembly, a dissociation pipe and a filtering assembly, the dissociation pipe is filled with a mixed solution of tissues to be dissociated and digestive juice; the dissociation assembly and the dissociation pipe are two mutually independent parts and have the functions of high-speed rotary shearing stirring and heating; the filtering assembly is arranged at the bottom of the dissociation pipe and is used for filtering a cell suspension. The dissociation assembly and the dissociation tube are two independent parts and are small in size, and an experimenter can hold the dissociation assembly and the dissociation tube by a single person for operation, so that the operation convenience is improved, the dissociation device is convenient to carry and place, various tissue dissociation processes can be flexibly carried out, and assistance of other experimenters or equipment is not needed; meanwhile, the shearing blade, the stirring fan blade and the connecting sleeve are used as integrally replaceable components, and the filtering assembly is also used as an integrally replaceable component, so that replacement is facilitated when the next batch of cell suspension is prepared.

Description

Technical Field

[0001] The utility model relates to the technical field of single-cell sequencing, and particularly relates to a tissue dissociation device. Background Art

[0002] Single-cell sequencing technology is to perform high-throughput sequencing on genomes, transcriptomes, etc. at the single-cell level, aiming to reveal the gene structure and expression status of single cells. With the reduction of sequencing costs and the continuous progress of technology, it has become a powerful tool in the fields of studying cell heterogeneity, disease mechanisms, etc., and is widely used in many fields such as oncology, microbiology, and neuroscience.

[0003] Moreover, the quality of the cell suspension is an important factor determining the results of single-cell sequencing. Unqualified cell suspensions may lead to failure in single-cell capture. For experiments with multiple samples in batches, different preparation and treatment methods of cell suspensions will also affect the results. Therefore, improving the effect and uniformity of single-cell suspension preparation can further promote the development and application of single-cell sequencing technology.

[0004] Currently, the preparation of cell suspensions for single-cell sequencing mainly relies on manual operation by experimenters. Different treatment methods and operating habits of different experimenters will have an uncontrollable impact on the final results; even the same experimenter is difficult to maintain exactly the same operation method between different batches, and mistakes occur due to factors such as state or external environment. These factors will greatly reduce the credibility of the results. Therefore, an automated tissue dissociation device is needed to ensure the effect and operation uniformity of cell suspension preparation through an automated process, while saving the labor and time costs of experiments.

[0005] The tissue dissociation devices disclosed in the prior art usually include a sample bottle and a grinding and filtering structure. The grinding and filtering structure includes a grinding member and a filter screen. The grinding end of the grinding member and the filter screen can enter the storage space of the sample bottle through the storage opening of the sample bottle, and divide the storage space into a tissue accommodation area and a cell suspension accommodation area. The grinding end in the sealed position can rotate to grind the biological tissue located in the tissue accommodation area. At this time, the cell suspension accommodation area is in a sealed state. When the grinding is completed and the grinding end moves to the filtering position, the cell suspension accommodation area is in a negative pressure state, and the cell suspension formed by grinding the biological tissue can pass through the filter screen for filtration and enter the cell suspension accommodation area for temporary storage. However, the tissue dissociation devices in the prior art still have some defects, such as the grinding and filtering structure is difficult to clean, and the device size is relatively large and not very convenient to use, etc. Summary of the Utility Model

[0006] The purpose of the utility model is: aiming at the deficiencies in the above background art, to provide a tissue dissociation device with convenient operation, easy to carry and place, capable of independently completing the cell suspension preparation process, and without the need for on-site cleaning.

[0007] To achieve the above object, the present utility model provides a tissue dissociation device, which includes a dissociation component, a dissociation tube, and a filtration component;

[0008] The dissociation tube is filled with a mixed solution of tissue to be dissociated and digestive fluid;

[0009] The dissociation component includes a mounting seat, a motor disposed within the mounting seat, a rotating shaft connected to the output end of the motor through a transmission structure, and a shearing blade and a stirring fan blade connected to the rotating shaft through a connecting member; the rotating shaft, the shearing blade, and the stirring fan blade are movably inserted into the dissociation tube;

[0010] The dissociation component further includes a heating portion for heating the mixed solution within the dissociation tube;

[0011] The filtration component is disposed at the bottom of the dissociation tube and is used for filtering the cell suspension.

[0012] Furthermore, the shearing blade and the stirring fan blade are both provided with multiple layers and are spaced apart in sequence.

[0013] Furthermore, the lower surface of the mounting seat is provided with an annular limiting groove, and the top edge of the dissociation tube is inserted into the limiting groove to form a limit.

[0014] Furthermore, the connecting member is a connecting sleeve, and the shearing blade and the stirring fan blade are both connected to the connecting sleeve to form an integral structure. The connecting sleeve is a tubular structure, and the size of the connecting sleeve matches that of the rotating shaft. The connecting sleeve is sleeved on the rotating shaft and can be quickly disassembled.

[0015] Furthermore, the heating portion includes a heating rod, and a resistance wire is disposed within the heating rod. The heating rod is inserted into the interior of the dissociation tube.

[0016] Furthermore, the rotating shaft is provided with a hollow structure, the heating rod is disposed within the rotating shaft and is independent of the rotating shaft, and the heat generated by the heating rod is transferred to the rotating shaft and then heats the mixed solution in direct contact with the rotating shaft.

[0017] Furthermore, the heating portion includes a heating sleeve, and a resistance wire is disposed within the heating sleeve. The heating sleeve is sleeved on the outside of the dissociation tube.

[0018] Furthermore, the filtration component includes a filtration tube, a filtration switch disposed within the filtration tube, and a filtration membrane. The filtration tube is detachably connected to the bottom of the dissociation tube. The filtration switch is used to control the communication state of the filtration tube, and the filtration membrane is used to filter the cell suspension.

[0019] Furthermore, the filter switch includes a fixed plate and a movable plate, and both the fixed plate and the movable plate are provided with openings. When the openings of the movable plate and the fixed plate are aligned, the filter switch is in a connected state; when the openings of the movable plate and the fixed plate are staggered, the filter switch is in a closed state.

[0020] Furthermore, the filter membrane is provided with two or more layers, the filter pore size of each layer of the filter membrane is different, and the filter pore size of the filter membrane decreases gradually from the top to the bottom.

[0021] The above solution of the utility model has the following beneficial effects:

[0022] The tissue dissociation device provided by the present invention dissociates the mixed solution in the dissociation tube to prepare a cell suspension based on the dissociation component. The dissociation component and the dissociation tube are two separate parts and are small in size. An experimenter can operate it by hand, thereby improving the convenience of operation. It is easy to carry and place, and can flexibly perform various tissue dissociation processes without the assistance of other experimenters or equipment.

[0023] The tissue dissociation device provided by the present invention has a shear blade, a stirring fan blade, and a connecting sleeve as an integrally replaceable component. When preparing the next batch of cell suspension, it is only necessary to remove the connecting sleeve from the rotating shaft and replace it with a new integral installation. There is no need for on-site cleaning, which further improves the convenience of operation for the experimenter.

[0024] The tissue dissociation device provided by the present invention has a filtration component that can be conveniently and reliably controlled, and uses a multi-layer filtration method to improve the filtration effect. At the same time, the entire component is also a replaceable component, which is also convenient for replacement when preparing the next batch of cell suspension.

[0025] Other beneficial effects of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0027] Figure 2 Schematic diagram of the dissociation component of the present invention;

[0028] Figure 3 This is a schematic diagram of the connection between the internal rotating shaft and the heating rod of the mounting base of the utility model;

[0029] Figure 4 This is a schematic diagram of the filter assembly of the present invention.

[0030] [Description of Reference Numerals]

[0031] 1 - dissociation tube; 2 - collection tube; 3 - mounting base; 4 - rotating shaft; 5 - shearing blade; 6 - stirring fan blade; 7 - limiting groove; 8 - connecting sleeve; 9 - heating rod; 10 - bearing; 11 - driven gear; 12 - driven pulley; 13 - connecting frame; 14 - filter tube; 15 - filter membrane; 16 - fixing plate; 17 - movable plate; 18 - opening; 19 - plug; 20 - speed button; 21 - temperature button; 22 - small display screen. Detailed implementation mode

[0032] To make the technical problems, technical solutions and advantages to be solved by the present utility model clearer, the following will be described in detail with reference to the accompanying drawings and specific embodiments. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model. In addition, the technical features involved in different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0033] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as a limitation of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0034] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", "connection" should be understood in a broad sense. For example, it can be a locking connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0035] Such as Figure 1 、 Figure 2As shown in the figure, an embodiment of the present utility model provides a tissue dissociation device, which includes a dissociation component, a dissociation tube 1, a filtration component, and a collection tube 2. Among them, the dissociation tube 1 is used to contain a mixture of tissue to be dissociated and digestive fluid. The dissociation component has the function of performing high-speed shearing and stirring on the mixture in the dissociation tube 1, so as to destroy the extracellular matrix, release cells from the extracellular matrix, and at the same time further destroy the connection between cells to obtain a cell suspension. Subsequently, the cell suspension is filtered through the filtration component to remove impurities and the like in the cell suspension, so as to obtain a cell suspension sample that can be used for single-cell sequencing.

[0036] At the same time, during the process of performing high-speed shearing and stirring on the mixture in the dissociation tube 1, it is also necessary to heat the mixture, so that the extracellular matrix can be destroyed more quickly and fully, and the efficiency and quality of cell suspension preparation can be improved. Therefore, the dissociation component also needs to have the function of heating.

[0037] Specifically in this embodiment, the dissociation component includes a mounting seat 3, a motor disposed in the mounting seat 3, a rotating shaft 4 connected to the output end of the motor through a transmission structure, and a shearing blade 5 and a stirring fan blade 6 connected to the rotating shaft 4 through a connecting member. Among them, when the motor is powered on, it can drive the rotating shaft 4 to rotate at a high speed, so that the shearing blade 5 and the stirring fan blade 6 rotate at a high speed to perform high-speed shearing and stirring on the mixture in the dissociation tube 1. As a preferred implementation manner, both the shearing blade 5 and the stirring fan blade 6 are provided with two or more layers and are distributed at intervals in sequence, and the top layer is the shearing blade 5. Therefore, the mixture can be sheared and stirred more fully under the action of the multi-layer shearing blade 5 and the stirring fan blade 6.

[0038] Among them, the dissociation component and the dissociation tube 1 are two independent parts. For different types or batches of samples, different dissociation tubes 1 need to be replaced to contain and prepare cell suspensions. Since the dissociation tube 1 can be made of hard plastic and the cost is very low, it can be directly used as a consumable, discarded after use and replaced with a new dissociation tube 1. During use, the rotating shaft 4 of the dissociation component and the connected shearing blade 5 and stirring fan blade 6 are inserted into the mixture in the dissociation tube 1, and the motor is started to drive the shearing blade 5 and the stirring fan blade 6 to rotate at a high speed in the mixture, so as to complete the high-speed shearing and stirring of the mixture.

[0039] It can be understood that the size of the dissociation component needs to be set as small as possible for convenient hand-held operation, so that a single experimenter can directly complete the treatment of the mixed solution in the dissociation tube 1 with both hands, improving the convenience of operation. During the operation, the experimenter holds the bottom of the dissociation tube 1 with the left hand (or right hand), and holds the mounting seat 3 with the right hand (or left hand), inserts the rotating shaft 4 and the connected shearing blade 5 and stirring fan blade 6 into the dissociation tube 1 until the top edge of the dissociation tube 1 abuts against the lower surface of the mounting seat 3, and then starts the operation, avoiding the shearing blade 5 and the stirring fan blade 6 not being fully inserted into the appropriate position of the mixed solution and splashing, etc.

[0040] As a preferred implementation manner, in this embodiment, a circular limiting groove 7 is further provided on the lower surface of the mounting seat 3. The limiting groove 7 is used for the top edge of the dissociation tube 1 to be inserted, to prevent the relative position of the dissociation tube 1 and the mounting seat 3 from deviating during the operation of the experimenter, resulting in the shearing blade 5 and the stirring fan blade 6 contacting the inner wall of the dissociation tube 1 and causing shutdown or damaging the structure of the dissociation tube 1. Among them, the width of the limiting groove 7 is slightly larger than the wall thickness of the dissociation tube 1, so as to facilitate the insertion of the top edge of the dissociation tube 1 and be able to fully limit the position. It should be noted that for dissociation tubes 1 of different sizes, different specifications of dissociation components need to be replaced, and the limiting groove 7 on the lower surface of the mounting seat 3 is matched with the dissociation tube 1 of the corresponding size.

[0041] As mentioned above, for different types or batches of samples, different dissociation tubes 1 need to be replaced. Since the shearing blade 5 and the stirring fan blade 6 on the rotating shaft 4 also come into contact with the sample (mixed solution), when processing the next batch of samples, the entire rotating shaft 4 including the shearing blade 5 and the stirring fan blade 6 also needs to be cleaned to remove the adhered mixed solution of the previous batch, so as to avoid the newly prepared cell suspension being contaminated and affecting the detection results.

[0042] Considering that there are multiple layers of the shearing blade 5 and the stirring fan blade 6 on the rotating shaft 4, and there are a large number of dead corners, etc., which makes it difficult to completely clean the mixed solution. Therefore, as a preferred implementation manner, in this embodiment, the shearing blade 5 and the stirring fan blade 6 also adopt a replaceable form, so that the experimenter does not need to clean the shearing blade 5 and the stirring fan blade 6 during the next batch of operations, but can directly replace them.

[0043] Specifically, the connecting member is set as a connecting sleeve 8, and both the shearing blade 5 and the stirring fan blade 6 are connected to the connecting sleeve 8. Preferably, an integral structure is adopted, which is an integral, independent and replaceable component. The connecting sleeve 8 itself is also a tubular structure, with dimensions matching those of the rotating shaft 4. It is made of a material with certain elasticity (or an interference fit connection is formed using hard plastic, etc.). The connecting sleeve 8 is sleeved from below the rotating shaft 4 and forms a fixed state with the rotating shaft 4 by relying on the elastic force, so that the rotating shaft 4 drives the shearing blade 5 and the stirring fan blade 6 to rotate synchronously at high speed during high-speed rotation. When replacement is needed, only the connecting sleeve 8 needs to be removed from the rotating shaft 4 and a new integral installation can be carried out, further improving the convenience of operation for the experimenters.

[0044] When adopting the integral structure of the shearing blade 5, the stirring fan blade 6 and the connecting sleeve 8, for the convenience of manufacturing, it can be considered that the stirring fan blade 6 is made of the same material as the connecting sleeve 8, and the tool holder of the shearing blade 5 is also made of the same material as the connecting sleeve 8. At the same time, a shearing edge made of a metal material is installed on the tool holder. Of course, if the shearing blade 5 is also completely made of the same material as the connecting sleeve 8 and the effect of shearing and preparing the cell suspension meets the standard after actual testing, then this scheme can also be adopted. Those skilled in the art can make a choice according to the manufacturing cost, actual test effect, etc. This embodiment only illustrates this rather than making a limitation.

[0045] In addition, in other embodiments, a scheme of setting a clamp on the upper part of the rotating shaft 4 or adopting an independent clamp component can also be adopted to fix the upper part of the connecting sleeve 8 to the rotating shaft 4, ensuring that the rotating shaft 4 drives the shearing blade 5 and the stirring fan blade 6 to rotate synchronously at high speed during high-speed rotation. This is also an optional implementation manner.

[0046] It should be noted that from the perspective of cost, since the cost of manufacturing the integral component of the shearing blade 5, the stirring fan blade 6 and the connecting sleeve 8 is relatively high, the component can be removed and collected after use, and then cleaned uniformly later, avoiding the cumbersome process and reduced efficiency caused by the need for cleaning during the operation of the experimenters.

[0047] In this embodiment, the heating function of the dissociation component can be realized in two ways. One of the heating methods is to heat inside the dissociation tube 1, that is, the heating source is directly in contact with the mixed liquid inside the dissociation tube 1. Specifically, the dissociation component further includes a heating rod 9. A resistance wire is arranged inside the heating rod 9. When the resistance wire is energized, it will generate heat, thereby heating the mixed liquid inside the dissociation tube 1.

[0048] Considering that there are multiple layers of shearing blades 5 and stirring fan blades 6 arranged in the dissociation tube 1, when the heating rod 9 is arranged outside the rotating shaft 4, it is easily obstructed by the shearing blades 5 and the stirring fan blades 6, and cannot sufficiently heat each layer position of the mixed liquid. Based on this, in this embodiment, the rotating shaft 4 is set as a hollow structure, the heating rod 9 is arranged inside the rotating shaft 4, and is independent of the rotating shaft 4, and remains fixed during the rotation of the rotating shaft 4. Therefore, the heat generated by the heating rod 9 can be transferred to the rotating shaft 4, and then heat the mixed liquid in direct contact with the rotating shaft 4. Among them, notches can be opened at some positions of the connecting sleeve 8 so that the mixed liquid can be in direct contact with the rotating shaft 4 at the notch position.

[0049] At the same time, as Figure 3 shown, at the position of the mounting seat 3, the top of the rotating shaft 4 is rotatably connected to the mounting seat 3 through a bearing 10. The transmission structure can adopt gear transmission or belt pulley transmission, that is, a driven gear 11 or a driven belt pulley 12 is installed at the top of the rotating shaft 4. The output shaft of the motor is meshed with the driven gear 11 through a driving gear, or connected to the driven belt pulley 12 through a driving belt pulley and a transmission belt to drive the rotating shaft 4 to rotate. At the same time, the top of the heating rod 9 extends out from the top of the rotating shaft 4 and is fixed through a connecting frame 13. At the same time, the circuit on the connecting frame 13 is connected to the resistance wire in the heating rod 9. Therefore, this method can ensure the rotation drive of the rotating shaft 4 and the heating energy supply of the heating rod 9.

[0050] Another heating method is to heat outside the dissociation tube 1, and transfer the heat to the internal mixed liquid through the tube wall of the dissociation tube 1. Specifically, the dissociation assembly further includes a heating sleeve, and a resistance wire is arranged inside the heating sleeve. The heating sleeve is connected to the mounting seat 3 through an external circuit, or an independent plug-in circuit is set for power-on. Among them, the heating sleeve can adopt a fixing method with magic tape. When installation is required, the heating sleeve is wound around the corresponding position of the dissociation tube 1, and then the heating sleeve is fixed tightly through the magic tape.

[0051] Since the effect of the external heating method is not as good as that of the internal heating method, and at the same time, when using the heating sleeve, the experimenter cannot see the condition of the mixed liquid in the dissociation tube 1 clearly, and in addition, the heating sleeve also makes the structure exposed outside more cumbersome, so the previous heating method is the preferred implementation method of this embodiment.

[0052] In this embodiment, the filtering assembly is used to filter the cell suspension prepared by dissociation after sufficient shearing and stirring, so as to remove impurities and the like in the cell suspension, and obtain a cell suspension sample that can be used for single-cell sequencing. At the same time, as Figure 4As shown, the filter assembly is arranged at the bottom of the dissociation tube 1, and specifically includes a filter tube 14, a filter switch arranged in the filter tube 14, and a filter membrane 15. Among them, the filter tube 14 is connected to the bottom of the dissociation tube 1, and the filter switch is used to control the connection state, so that it is closed during the stirring process of the mixed liquid to prevent the mixed liquid from entering the filter tube 14. When filtering is required, the filter switch is turned on to allow the mixed liquid to enter the filter tube 14, and the large particles of impurities in the cell suspension are filtered and removed by the filter membrane 15. The filtered cell suspension eventually flows out from the bottom of the filter tube 14, and the cell suspension can be collected by the collection tube 2 for subsequent detection.

[0053] As a preferred embodiment, the filter switch in this embodiment adopts a rotary switching method. The filter switch includes a fixed plate 16 and a movable plate 17. At the same time, an opening 18 is provided on each of the fixed plate 16 and the movable plate 17. When the movable plate 17 rotates until the opening 18 is aligned with the fixed plate 16, the filter switch is in a connected state, allowing the mixed liquid in the dissociation tube 1 to enter the position of the filter membrane 15 located at the lower layer and be filtered. When the movable plate 17 rotates until the opening 18 is offset from the fixed plate 16, the filter switch is in a closed state, preventing the mixed liquid in the dissociation tube 1 from flowing downward and being filtered.

[0054] It should be noted that, in this embodiment, the edges of the fixed plate 16, the movable plate 17, and the filter membrane 15 are all provided with sealing rings or similar structures to seal with the inner wall of the filter tube 14. At the same time, the movable plate 17 and the fixed plate 16 are arranged in close contact, and the opening 18 of the movable plate 17 is provided with a sealing ring and in contact with the fixed plate 16 to ensure that when the openings 18 of the movable plate 17 and the fixed plate 16 are staggered, the mixed liquid cannot pass through the gap between the movable plate 17 and the fixed plate 16, thereby achieving an absolute sealing effect.

[0055] Of course, in other embodiments, the two movable plates 17 may be opened and closed by horizontal movement, and controlled by a toggle portion extending to the outside of the filter tube 14 .

[0056] As a preferred embodiment, in this embodiment, the filter membrane 15 is provided with more than two layers, and the filtration pore size of each layer of filter membrane 15 is different. The filtration pore size of the filter membrane 15 gradually decreases from top to bottom. For example, the filtration pore size of the upper filter membrane 15 is 70μm, and the filtration pore size of the lower filter membrane 15 is 30μm, so as to achieve layered filtration and further improve the filtration degree and filtration effect.

[0057] As a preferred embodiment, the bottom of the filter tube 14 in this embodiment is set to be conical or in other forms with a reduced outer diameter, so that the experimenter can quickly insert the bottom of the filter tube 14 into the collection tube 2 to collect the filtered cell suspension, which can also improve the convenience of operation. During the dissociation of the mixed solution, the collection tube 2 is temporarily placed on the side, and the experimenter holds the bottom of the dissociation tube 1 and the mounting base 3 with both hands for operation. After the dissociation is completed, the experimenter can first separate and place the dissociation component from the dissociation tube 1, and then hold the bottom of the dissociation tube 1 and the collection tube 2 with both hands. After inserting the bottom of the filter tube 14 into the collection tube 2, the filter switch is turned on for filtration. The whole operation can be directly completed by one experimenter without the assistance of other experimenters.

[0058] Similarly, for samples of different types or batches, the filter component also comes into contact with the samples. When processing the next batch of samples, it is also necessary to clean the entire filter component to prevent the newly prepared cell suspension from being contaminated and affecting the test results. Based on this, in this embodiment, the filter tube 14 and the dissociation tube 1 are still detachably connected. The top of the filter tube 14 is provided with a connecting section with a reduced size. The connecting section is inserted into the bottom of the dissociation tube 1 and fixed by interference fit or other means. At the same time, a sealing ring can be set to further ensure the sealing performance. After the filtration of the previous batch is completed, the filter tube 14 is detached from the dissociation tube 1. Considering that the cost of the filter component is relatively high, the filter component is collected for subsequent unified cleaning and reuse.

[0059] As described above, the tissue dissociation device provided in this embodiment has functions such as high-speed shear stirring, heating, and filtration of the dissociation solution. Among them, the high-speed shear stirring driven by the motor and the heating by the resistance wire both require power supply. Therefore, a control board is also provided in the mounting base 3. The control board serves as a control system to control the motor and the resistance wire. At the same time, a battery is also provided in the mounting base 3 to supply power to the control board, the motor, the resistance wire, etc. When the battery power is insufficient, it can be charged. Of course, it can also be used in a direct plug-in manner, that is, a plug 19 is provided and connected to the power supply in the mounting base 3. When the plug 19 is connected to the socket, the power supply supplies power to the control board, the motor, the resistance wire, etc. In addition, a speed button 20, a temperature button 21, a small display screen 22, etc. are also provided on the mounting base 3. The speed button 20 is used to turn on and off the motor and switch the speed gears, the temperature button 21 is used to adjust the temperature gears, and the small display screen 22 is used to display the speed gears, temperature gears, etc.

[0060] In short, the tissue dissociation device provided in this embodiment has the advantages of small volume, simple structure, convenient to carry and place. The experimenter can independently complete the preparation process of the cell suspension without the assistance of other instrument devices, with convenient operation, time-saving and labor-saving, and ensuring the consistency of results.

[0061] The technical features of the above embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0062] The above embodiments only represent several implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation to the scope of the application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several deformations and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A tissue dissociation device, characterized in that, It includes a dissociation component, a dissociation tube (1), and a filtration component; The dissociation tube (1) contains a mixture of tissue to be dissociated and digestive fluid; The dissociation assembly comprises a mounting seat (3), a motor arranged in the mounting seat (3), a rotating shaft (4) connected to the output end of the motor via a transmission structure, and a shearing blade (5) and a stirring blade (6) connected to the rotating shaft (4) via a connecting piece; the rotating shaft (4), the shearing blade (5), and the stirring blade (6) are movably inserted into the dissociation tube (1); The dissociation assembly further comprises a heating portion, which is used to heat the mixed liquid in the dissociation tube (1); The filtering component is arranged at the bottom of the dissociation tube (1), and is used for filtering the cell suspension.

2. The tissue dissociation device according to claim 1, wherein, The shear blades (5) and the stirring blades (6) are both provided with multiple layers and are sequentially spaced and distributed.

3. The tissue dissociation device according to claim 1, characterized in that, An annular limiting groove (7) is provided on the lower surface of the mounting seat (3), and the top edge of the dissociation tube (1) is plugged into the limiting groove (7) for limiting position.

4. The tissue dissociation device according to claim 1, wherein The connecting piece is a connecting sleeve (8), and the shear blade (5) and the stirring blade (6) are both connected to the connecting sleeve (8) to form an integrated structure. The connecting sleeve (8) is a tubular structure, and the size of the connecting sleeve (8) matches the rotating shaft (4). The connecting sleeve (8) is sleeved with the rotating shaft (4).

5. The tissue dissociation device according to claim 1, characterized in that, The heating portion comprises a heating rod (9), a resistance wire is arranged inside the heating rod (9), and the heating rod (9) is inserted into the interior of the dissociation tube (1).

6. The tissue dissociation device according to claim 5, wherein, The rotating shaft (4) is configured as a hollow structure, and the heating rod (9) is disposed inside the rotating shaft (4) and is independent of the rotating shaft (4).

7. The tissue dissociation device according to claim 1, wherein, The heating part comprises a heating jacket, a resistance wire is arranged in the heating jacket, and the heating jacket is arranged outside the dissociation tube (1).

8. An organization dissociation device according to claim 1, characterized in that, The filtration assembly comprises a filtration tube (14), a filtration switch arranged in the filtration tube (14), and a filtration membrane (15); the filtration tube (14) is detachably connected to the bottom of the dissociation tube (1); the filtration switch is used to control the connection state of the filtration tube (14); and the filtration membrane (15) is used to filter the cell suspension.

9. The tissue dissociation device according to claim 8, wherein, The filter switch comprises a fixed plate (16) and a movable plate (17), and both the fixed plate (16) and the movable plate (17) are provided with an opening (18). When the movable plate (17) is aligned with the opening (18) of the fixed plate (16), the filter switch is in a connected state; when the movable plate (17) is offset from the opening (18) of the fixed plate (16), the filter switch is in a closed state.

10. The tissue dissociation device according to claim 8, characterized in that, The filter membrane (15) is provided with two or more layers, and the filter pore size of each layer of the filter membrane (15) is different, and the filter pore size of the filter membrane (15) decreases gradually from the top to the bottom.

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