Sample culture detection system

Abstract

The utility model relates to a sample culture and detection system, which comprises an incubation culture device, a detection device and a control device, and the fluorescence detection device is used for detecting the sample in the incubation culture device. The sample culture and detection system executes the sample culture and detection method; the sample culture detection method comprises the following steps: incubating a sample in the incubation culture device by the incubation culture device; the fluorescence detection device is used for detecting the sample. The sample culture and detection system is used for incubating and detecting a sample so as to improve the detection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro diagnosis, in particular to a sample culture detection system. Background Art

[0002] Liquid culture of Mycobacterium tuberculosis (M. tuberculosis) has become the gold standard for tuberculosis diagnosis due to its high specificity. Liquid culture technology utilizes fluorescence intensity detection to analyze and automatically interpret the changes in fluorescence intensity caused by oxygen consumption during mycobacterial growth. This technology significantly shortens the culturing time for tuberculosis bacteria, saving patients valuable time for treatment.

[0003] However, in the prior art, sample incubation and detection cannot be performed simultaneously. Sample incubation needs to be performed in an incubation device, and sample detection needs to be performed in a fluorescence detection device.

[0004] The equipment is inconvenient to operate, and the detailed results of relevant samples (such as the time of reporting positive results, etc.) can only be viewed through printed reports.

[0005] In addition, existing fluorescence detection devices often include multiple fluorescence detection modules to meet high-throughput detection needs, that is, one fluorescence detection module corresponds to one sample. Since the equipment is generally small in size and each fluorescence detection module is relatively large, this design can only meet the needs of a certain number of samples. For a large number of samples, the detection device is difficult to meet the requirements. At the same time, the large number of fluorescence detection devices leads to relatively high costs.

[0006] In addition, the existing technology cannot detect whether there is a sample at the location where the sample is placed, which will lead to invalid detection.

[0007] In addition, the sample to be tested often requires a suitable external temperature environment before or during the test to promote its growth, so it is often necessary to equip it with a heating device to control the temperature of its environment space. However, existing incubation and culture devices have the following shortcomings:

[0008] 1. The incubator-like device has a large space, but the detection throughput is small, and the temperature uniformity in the incubator is poor, which leads to deviations in the test results;

[0009] 2. The heating rate is slow, resulting in low detection efficiency. Utility Model Content

[0010] One purpose of the present invention is to provide a sample culture detection system to at least solve one of the above technical problems.

[0011] To achieve the above objectives, the first aspect of the present invention provides a sample culture detection system, comprising:

[0012] An incubation and culture device, used for incubating samples;

[0013] A fluorescence detection device for detecting samples within the incubation and culturing device.

[0014] Optionally, the incubation and culturing device includes:

[0015] A box body;

[0016] An incubator for accommodating samples and capable of being arranged within the box body;

[0017] A heating mechanism arranged within the box body, the heating mechanism being capable of heating samples within the incubator.

[0018] Optionally, the box body includes a flow guiding structure, the flow guiding structure enabling air to circulate between the heating mechanism and the incubator.

[0019] Optionally, the flow guiding structure includes an air supply duct and a return air duct, the heating mechanism, the air supply duct, the incubator, and the return air duct are sequentially connected and communicate to form an air circulation loop.

[0020] Optionally, the air supply outlet end of the air supply duct communicates with a first side of the incubator, the air return inlet end of the return air duct communicates with a second side of the incubator, and the first side and the second side are oppositely arranged.

[0021] Optionally, the box body further includes a first box wall and a second box wall, the air supply duct is arranged within the first box wall, and the return air duct is arranged within the second box wall.

[0022] Optionally, the first box wall and the second box wall are oppositely arranged; and / or

[0023] The incubator is arranged between the first box wall and the second box wall, and the first box wall is oppositely arranged with the first side of the incubator, and the second side of the incubator is oppositely arranged with the second box wall.

[0024] Optionally, the air supply outlet end includes a plurality of air inlet holes arranged at intervals; and / or, the air return inlet end includes a plurality of air outlet holes arranged at intervals.

[0025] Optionally, the flow guiding structure further includes an air supply buffer portion for buffering hot air and / or making the hot air distribution uniform, the air supply duct and the air supply outlet end communicate through the air supply buffer portion; and / or

[0026] The flow guiding structure further includes a return air buffer portion for buffering air, the return air duct and the air return inlet end communicate through the return air buffer portion.

[0027] Optionally, at least one air supply outlet end is correspondingly arranged for one incubator; and / or

[0028] At least one of the incubators is correspondingly provided with at least one of the return air inlet ends.

[0029] Optionally, the heating mechanism includes an air driving member for driving air to flow between the heating mechanism and the incubator.

[0030] Optionally, the position of the box body near the bottom of the box has an accommodation space, the heating mechanism is arranged in the accommodation space, and the accommodation space is communicated with the diversion structure.

[0031] Optionally, the incubator includes a first housing capable of accommodating a culture tube for a sample, and at least the part of the culture tube accommodating the sample is located inside the first housing.

[0032] Optionally, the first housing is provided with a plurality of holes for inserting the culture tube.

[0033] Optionally, the first housing includes a top wall and a positioning plate arranged on the lower side of the top wall, the holes are opened on the top wall, the positioning plate is provided with positioning holes, and the culture tube can be inserted into the positioning holes.

[0034] Optionally, the incubator further includes a calibration tube arranged inside the first housing for calibrating the fluorescence detection device.

[0035] Optionally, the first housing includes a first side wall and a second side wall arranged opposite to each other, the air heated by the heating mechanism can enter the first housing through the first side wall, and the air inside the first housing can flow out through the second side wall.

[0036] Optionally, the first housing further includes a bottom wall connected to the first side wall and the second side wall, the bottom wall is provided with a plurality of first air holes; and / or

[0037] Second air holes are provided on both the first side wall and / or the second side wall.

[0038] Optionally, the incubation and culture device further includes a fresh air mechanism connected to the box body for filtering air, and outside air enters the box body and / or the heating mechanism through the fresh air mechanism.

[0039] Optionally, the heating mechanism includes a second housing and a second heating unit arranged inside the second housing.

[0040] Optionally, the heating mechanism further includes a first temperature detection unit and / or a temperature protection unit arranged inside the second housing.

[0041] Optionally, the incubator further includes a second temperature detection unit for detecting the temperature inside the incubator.

[0042] Optionally, the number of the incubators is one or more.

[0043] Optionally, the box body further includes a partition for separating two adjacent incubators.

[0044] Optionally, the incubator further includes a first heating unit for heating the samples inside the incubator.

[0045] Optionally, the fluorescence detection device performs fluorescence detection through the bottom of the culture tube.

[0046] Optionally, the fluorescence detection device includes a fluorescence detection module and a driving mechanism for driving the fluorescence detection module to move so that the fluorescence detection module can detect multiple samples located at different positions in the incubator respectively.

[0047] Optionally, the driving mechanism is used to drive the fluorescence detection module to reciprocate along a first direction and a second direction which are perpendicular to each other.

[0048] Optionally, the fluorescence detection device further includes an in-position detection mechanism connected to the driving mechanism for detecting whether samples exist at multiple preset positions for placing samples.

[0049] Optionally, the in-position detection mechanism includes one or more in-position detection modules.

[0050] Optionally, when the number of the in-position detection modules is multiple, the multiple in-position detection modules are arranged in sequence along the second direction, and the driving mechanism is used to drive the multiple in-position detection modules to move along the first direction.

[0051] Optionally, the multiple samples in the incubator are arranged in an array along the first direction and the second direction.

[0052] Optionally, the sample culture and detection system further includes a scanning module for scanning the identification part on the culture tube.

[0053] Optionally, the sample culture and detection system further includes a user terminal electrically connected to the incubation and culture device, the fluorescence detection device and the scanning module.

[0054] Optionally, the user terminal includes an input / output module and a control module, and the control module is electrically connected to the input / output module, the incubation and culture device, the fluorescence detection device and the scanning module.

[0055] Optionally, the user terminal is a terminal based on the Android system.

[0056] Optionally, the first direction and the second direction are located in the horizontal plane.

[0057] As can be seen from the above, in the technical solution provided by the present utility model, the sample culture detection system includes an incubation and culture device and a fluorescence detection device. The fluorescence detection device can detect the sample while the incubation and culture device incubates the sample, thereby effectively improving the detection efficiency.

[0058] The sample culture detection system may further include a user terminal. The user terminal is electrically connected to the incubation and culture device and the scanning module. The user terminal can display the temperature in each incubator, and can also adjust the target temperature in the incubator by clicking on the user terminal, and then control the opening and closing of the fresh air mechanism and the power of the heating mechanism to adjust the temperature. After the scanning module performs a scanning operation on the culture tube, the placement position of the tube can be displayed on the user terminal, thereby facilitating subsequent tube placement and tube removal operations, and facilitating real-time display of the detection results.

[0059] Each layer of incubator in the incubation and culture device can incubate 400 samples. Setting three incubators can meet the requirement of incubating 1200 samples simultaneously, thereby meeting the high-throughput requirement.

[0060] The incubation and culture device can accurately control the temperature in a large-capacity box body, and ensure its temperature accuracy and uniformity. At the same time, it is convenient for the fluorescence detection device embedded in the incubation and culture device to perform synchronous detection, improving the detection efficiency.

[0061] In the initial rising stage, heating is carried out by the heating mechanism and the first heating unit, thereby realizing rapid temperature rise. When the temperature in a single incubator is lower than the target temperature, the sample in the incubator can be heated by separately controlling the corresponding first heating unit, thereby further increasing the temperature rise speed and improving the detection efficiency. The first heating unit can independently adjust the temperature in the corresponding incubator, thereby accurately compensating the temperature in the incubator. The incubation and culture device can accurately control the temperature compensated by the first heating unit, and at the same time cooperate with the diversion structure, so as to quickly make the temperature in the incubator reach the target temperature, and make the temperature at each position point in the incubator reach the set target temperature ±1.5°C, ensuring the temperature uniformity in the incubator.

[0062] The first heating unit, the heating mechanism, the fresh air mechanism and the diversion structure perform rapid temperature control, so as to make the temperature at each hole position in the incubator reach the incubation temperature condition and meet the requirement of rapid incubation and culture.

[0063] The driving mechanism can move the fluorescence detection module to different positions, so as to detect samples at different positions. That is, one fluorescence detection module can correspond to multiple samples. The high-speed fluorescence detection device has a large detection throughput, low equipment cost, and small equipment volume, solving the problem in the prior art that one fluorescence detection module needs to correspond to one sample, resulting in a small detection throughput and high equipment cost. Through the movement of the in-situ detection mechanism and the fluorescence detection module, and through the cooperation of the in-situ detection mechanism and the fluorescence detection module, the fluorescence detection method can adapt to high-throughput detection.

[0064] The high-speed fluorescence detection device further includes an in-situ detection mechanism connected to the driving mechanism. The in-situ detection mechanism is used to detect whether there are samples at multiple preset positions. The in-situ detection mechanism can pre-identify whether there are samples. The pre-identification function of the in-situ detection mechanism is integrated with the fluorescence detection function. As a new application method, it can effectively improve the detection efficiency and subsequent data processing efficiency. Especially during high-throughput detection, it can effectively improve the subsequent data processing efficiency. Using the high-speed fluorescence detection device provided by the embodiment of the present utility model to complete the test of 1200 well positions only takes about 10 minutes. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] Figure 1 is a schematic structural diagram of the incubation and culture device provided by the embodiment of the present utility model;

[0066] Figure 2a is a partial schematic structure of the incubation and culture device provided by the embodiment of the present utility model Figure 1 ;

[0067] Figure 2b is Figure 2a a partial enlarged view at E in

[0068] Figure 2c is Figure 2a a partial enlarged view at F in

[0069] Figure 3 is a second partial schematic structure diagram of the incubation and culture device provided by the embodiment of the present utility model;

[0070] Figure 4a is a partial schematic structure diagram of the incubation and culture device provided by the embodiment of the present utility model;

[0071] Figure 4b is Figure 4a a cross-sectional view taken along line B-B in

[0072] Figure 4c is the explosion of the incubation and culture device provided by the embodiment of the present utility model Figure 1 ;

[0073] Figure 4dIt is a schematic structural diagram of another perspective of the incubation and culture device provided by the embodiment of the present utility model;

[0074] Figure 4e It is Figure 4d a cross-sectional view taken at C-C in;

[0075] Figure 4f It is Figure 4e a partially enlarged view at N in;

[0076] Figure 5a It is the second explosion diagram of the incubation and culture device provided by the embodiment of the present utility model;

[0077] Figure 5b It is Figure 5a a partially enlarged view at G in;

[0078] Figure 5c It is Figure 5a a partially enlarged view at H in;

[0079] Figure 5d It is Figure 5a a partially enlarged view at K in;

[0080] Figure 6 It is Figure 3 a partially enlarged view at A in;

[0081] Figure 7 It is a schematic diagram of a partial structure of the incubation and culture device provided by the embodiment of the present utility model Figure 3 ;

[0082] Figure 8 It is Figure 7 a partially enlarged view at B in;

[0083] Figure 9 It is a schematic structural diagram of the return air buffer part provided by the embodiment of the present utility model;

[0084] Figure 10 It is a schematic structural diagram of the air supply buffer part provided by the embodiment of the present utility model;

[0085] Figure 11 It is a schematic structural diagram of the incubator provided by the embodiment of the present utility model;

[0086] Figure 12 It is a schematic structural diagram of another perspective of the incubator provided by the embodiment of the present utility model;

[0087] Figure 13 It is a schematic diagram of a partial structure of the incubator provided by the embodiment of the present utility model;

[0088] Figure 14 It is Figure 13 a partially enlarged view at C in;

[0089] Figure 15a is an exploded view of the incubator provided by the embodiment of the present utility model;

[0090] Figure 15b is a partial structural schematic diagram of the incubator provided by the embodiment of the present utility model;

[0091] Figure 16a is a partial structural schematic of the fluorescence detection device provided by the embodiment of the present utility model Figure 1 ;

[0092] Figure 16b is Figure 16a a partial enlarged view of the D position in;

[0093] Figure 16c is Figure 16a a partial enlarged view of the L position in;

[0094] Figure 17a is a second partial structural schematic diagram of the fluorescence detection device provided by the embodiment of the present utility model;

[0095] Figure 17b is Figure 17a a partial enlarged view of the M position in;

[0096] Figure 18 is the sample culture and detection method flow provided by the embodiment of the present utility model Figure 1 ;

[0097] Figure 19 is the second sample culture and detection method flow chart provided by the embodiment of the present utility model.

[0098] In the figure:

[0099] 1. Incubator;

[0100] 10. Fluorescence detection device;

[0101] 11. Fluorescence detection module;

[0102] 12. Driving mechanism; 121. First driving component; 1211. First driving part; 1212. First driving pulley; 1213. First driven pulley; 1214. First belt; 122. Second driving component; 1221. Installation part; 1222. Second driving pulley; 1223. Second driven pulley; 1224. First belt; 1225. Tensioning pulley; 123. First guiding component; 1231. First guide rail; 1232. First guiding block; 124. Second guiding component; 1241. Second guide rail; 1242. Second guiding block;

[0103] 13. In-position detection mechanism; 131. In-position detection module; 132. In-position detection mounting plate;

[0104] 15. First housing; 151. First side wall; 152. Second side wall; 1521. Second air hole; 153. Bottom wall; 1531. First air hole; 154. Top wall; 1541. Hole position; 1542. Upper top plate; 1543. Lower top plate;

[0105] 16. First heating unit; 17. First side; 18. Second side; 19. Positioning plate; 191. Positioning hole;

[0106] 2. Box body;

[0107] 21. Flow guiding structure; 211. Air supply duct; 212. Air supply outlet end; 2121. Air inlet hole; 213. Air return duct; 214. Air return outlet end; 215. Air return inlet end; 2151. Air outlet hole; 216. Air supply inlet end;

[0108] 22. Box bottom; 23. Accommodating space;

[0109] 24. First box wall; 241. First inner plate; 242. First outer plate;

[0110] 25. Second box wall; 251. Second inner plate; 252. Second outer plate;

[0111] 26. Air supply buffer part; 261. First buffer frame; 262. First buffer space; 263. First partition part; 264. Air supply port; 265. First opening; 266. First ventilation hole; 267. Air supply pipe;

[0112] 27. Air return buffer part; 271. Second buffer frame; 272. Second buffer space; 273. Second partition part; 274. Air return port; 275. Second opening; 276. Second ventilation hole; 277. Air return pipe;

[0113] 28. Partition board;

[0114] 29. Rear box wall; 291. Third outer plate; 2911. Heat dissipation hole; 292. Intermediate plate; 293. Third inner plate;

[0115] 3. Heating mechanism; 31. Air driving part; 311. Exhaust fan; 312. Blower; 32. Second housing;

[0116] 4. Fresh air mechanism; 5. Heat dissipation mechanism; 6. Scanning module; 7. Heating power supply; 8. User terminal;

[0117] 100. Culture tube. Detailed implementation mode

[0118] The technical solution of the present utility model will be further described below in conjunction with the accompanying drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present utility model, rather than limiting the present utility model. In addition, it should be noted that for the convenience of description, only the parts related to the present utility model are shown in the drawings, rather than all of them.

[0119] In the present utility model, some orientation words are defined. In the case of no contrary description, the orientation words such as "upper", "lower", "left", "right", "inner", and "outer" are used for the convenience of understanding, and thus do not constitute a limitation on the protection scope of the present utility model.

[0120] In the present utility model, unless otherwise clearly specified and limited, the first feature being "on" or "under" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features between them. Moreover, the first feature being "above", "over" and "on" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "under" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0121] In the description of the present utility model, unless otherwise clearly specified and limited, the terms "connected", "connected to", and "fixed" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0122] This embodiment provides a sample culture and detection system for incubating and detecting samples to improve the detection efficiency.

[0123] As Figure 1 、 Figure 13 and Figure 15a 、 Figure 15b shown, the sample culture and detection system provided in this embodiment includes an incubation and culture device and a fluorescence detection device 10. The incubation and culture device is used to incubate samples, and the fluorescence detection device 10 is used to detect the samples in the incubation and culture device. The fluorescence detection device 10 can detect the samples while the incubation and culture device incubates the samples, thereby effectively improving the detection efficiency.

[0124] The incubation and culture device may include a box body 2, an incubator 1, and a heating mechanism 3. The incubator 1 is used to hold samples and can be disposed inside the box body 2. The heating mechanism 3 is disposed in the box body 2, and the heating mechanism 3 can heat the samples inside the incubator 1. Both the heating mechanism 3 and the incubator 1 are disposed inside the box body 2. The heating mechanism 3 can heat all the samples, enabling rapid temperature rise of the samples.

[0125] As Figure 1 - shown in Figure 4, the incubator 1 can enter the box body 2 by pulling. After the incubator 1 is pulled out from the box body 2, samples can be placed into the incubator 1 or taken out from the incubator 1. When the incubator 1 is pushed into the box body 2, the samples can be located in the box body 2 and heated by the heating mechanism 3.

[0126] In this embodiment, the box body 2 can be provided with three incubators 1. The three incubators 1 can be arranged in sequence along the vertical direction Z, thereby increasing the detection throughput. Of course, in other alternative embodiments, the number of incubators 1 is not limited to this. It can be more than three or less than three. For example, the number of incubators 1 can be one or two, or four or five, etc. When the number of incubators 1 is multiple, the multiple incubators 1 can also be placed in sequence along the horizontal direction. In this embodiment, the heating mechanism 3 can heat all the incubators 1, rapidly raising the temperature of each incubator 1 and improving the detection efficiency.

[0127] The box body 2 can be generally a cuboid. As Figure 2a shown, the box body 2 can include a first box wall 24 and a second box wall 25. The first box wall 24 and the second box wall 25 can be oppositely arranged.

[0128] The box body 2 can further include a box bottom 22. The box bottom 22 is connected to the lower ends of the first box wall 24 and the second box wall 25.

[0129] As Figure 3 shown, the box body 2 can further include a rear box wall 29. The rear box wall 29 is connected to the rear ends of the first box wall 24 and the second box wall 25. The front end of the box body 2 is open for the incubator 1 to be inserted into the box body 2. When the incubator 1 is inserted into the box body 2, the incubator 1 is located between the first box wall 24 and the second box wall 25.

[0130] As shown in Figure 4, the incubation and culture device can further include a fresh air mechanism 4 connected to the box body 2. The fresh air mechanism 4 is used to filter air. The outside air enters the box body 2 and / or the heating mechanism 3 through the fresh air mechanism 4, thereby ensuring clean air inside the box body 2. Optionally, the fresh air mechanism 4 is disposed at the lower end of the box body 2, that is, the outside air enters the box body 2 from the lower end.

[0131] The heating mechanism 3 can be arranged on one side of the fresh air mechanism 4 in the horizontal direction. After the outside air passes through the fresh air mechanism 4, it can directly enter the heating mechanism 3, and then the air can flow smoothly to the incubator 1. Since the low-temperature air tends to sink, the low-temperature outside air enters the box body 2 and the heating mechanism 3 from the lower end, so as to avoid the low-temperature outside air entering the incubator 1 without passing through the heating mechanism 2.

[0132] As Figure 2a and Figure 3 shown, optionally, the heating mechanism 3 can include a second housing 32 and a second heating unit (not shown in the figure), and the second heating unit is arranged in the second housing 32. Optionally, one side of the second housing 32 facing the fresh air mechanism 4 is provided with an opening so that the air passing through the fresh air mechanism 4 enters the second housing 32 through the opening. Optionally, the second heating unit can be a PTC heating element or a resistance wire, etc.

[0133] To increase the flow rate of air in the box body 2, optionally, the heating mechanism 3 can include an air driving member 31, and the air driving member 31 is used to drive the air to flow between the heating mechanism 3 and the incubator 1, so that the air driving member 31 can quickly send the heated air to the incubator 1, increasing the heating rate in the incubator 1 and the temperature adjustment rate in the incubator 1.

[0134] As Figure 2a 、 Figure 2b and Figure 2c shown, the air driving member 31 can include a blower. Specifically, a blower 312 is arranged on one side of the second housing 32, and an exhaust fan 311 is arranged on the other side. The blower 312 and the exhaust fan 311 are arranged opposite to each other. The exhaust fan 311 can draw the air in the incubator 1 back to the heating mechanism 3, and the blower 312 can blow the air heated by the heating mechanism 3 into the incubator 1, thereby further increasing the flow rate of the air flow and increasing the heating rate in the incubator 1.

[0135] To facilitate controlling the power of the second heating unit, the heating mechanism 3 can further include a first temperature detection unit and / or a temperature protection unit arranged in the second housing 32. The first temperature detection unit is used to detect the air temperature in the second housing 32. When the air in the second housing 32 is lower than the preset temperature, the power of the second heating unit can be increased. When the temperature in the second housing 32 is higher than the preset temperature, the power of the second heating unit can be decreased. When the temperature in the second housing 32 is too high, the temperature protection unit is turned on to stop the second heating unit from working, and at the same time, the fresh air mechanism 4 can also be opened. Optionally, the temperature protection unit can be a temperature protector in the prior art. When the temperature is too high, the temperature protector can automatically cut off the power supply for the second heating unit.

[0136] As Figure 5a andFigure 5b As shown, the box body 2 may further include a heat dissipation mechanism 5, and the heat dissipation mechanism 5 is used to dissipate heat from the heating mechanism 3, so as to prevent the heating mechanism 3 from overheating. The heat dissipation mechanism 5 may be a fan.

[0137] As Figure 4d - Figure 5a shown, specifically, the heat dissipation mechanism 5 is connected to the rear box wall 29. Optionally, the rear box wall 29 may include a third outer plate 291 and a third inner plate 293. The third outer plate 291 is located outside the third inner plate 293. The heat dissipation mechanism 5 may be connected to the third outer plate 291 and located inside the third outer plate 291. The third outer plate 291 may be provided with heat dissipation holes 2911 (as Figure 5c ), and the heat dissipation mechanism 5 is opposite to the heat dissipation holes 2911 to dissipate hot air.

[0138] The rear box wall 29 may further include an intermediate plate 292. The intermediate plate 292 is located between the third outer plate 291 and the third inner plate 293. The intermediate plate 292 may be connected to the heating power supply 7 (as Figure 5d ). A plurality of heating power supplies 7 may be provided to supply power to the heating mechanism 3 and a plurality of first heating units 16 (detailed later) respectively. The intermediate plate 292 can reduce or eliminate the influence of the heat generated by the heating power supply 7 on the incubator 1.

[0139] As Figure 2a - Figure 8 shown, optionally, the box body 2 may include a flow guiding structure 21, and the flow guiding structure 21 enables air to circulate between the heating mechanism 3 and the incubator 1. The flow guiding structure 21 can guide the air in the box body 2, increase the air flow rate, and enable the air to circulate along a preset path, so that each position point in the incubator 1 can reach the set target temperature ±1.5 °C, ensuring the temperature uniformity in the incubator 1.

[0140] As Figure 3 shown, the box body 2 has a receiving space 23 near the bottom 22 of the box. Optionally, the box body 2 may further include a partition 28. A receiving space 23 is formed between the partition 28 and the bottom 22 of the box body 2. The heating mechanism 3 is arranged in the receiving space 23. The receiving space 23 is communicated with the flow guiding structure 21, so that the heated air in the receiving space 23 enters the incubator 1 through the flow guiding structure 21, and the air in the incubator 1 flows back into the receiving space 23. The receiving space 23 can prevent unheated air from directly entering the incubator 1, ensuring the temperature accuracy and temperature uniformity in the incubator 1.

[0141] Optionally, there can be multiple partitions 28, and the partitions 28 can also be used to separate two adjacent incubators 1 to reduce or avoid the exchange of airflow between the incubators 1, while ensuring that the airflow flows in a preset direction, that is, the airflow flows from the heating mechanism 3 into the guide structure 21, and then flows into the incubators 1 respectively, and finally flows back to the heating mechanism 3 through the air circulation mechanism. This can ensure the temperature uniformity in each incubator 1 and improve the success rate of sample incubation.

[0142] like Figure 4a - Figure 4c As shown, the air guide structure 21 may optionally include an air supply duct 211 and an air return duct 213. The heating mechanism 3, the air supply duct 211, the incubator 1, and the air return duct 213 are sequentially connected to form a loop. The air supply duct 211 and the air return duct 213 may define the air flow path, thereby guiding the airflow.

[0143] Optionally, the supply air duct 211 is arranged in the first box wall 24, and the return air duct 213 is arranged in the second box wall 25. In this embodiment, the first box wall 24 and the second box wall 25 are arranged relative to each other, so that the supply air duct 211 and the return air duct 213 are also arranged relative to each other. The supply air duct 211 and the return air duct 213 are independently arranged, separated from each other, and do not interfere with each other.

[0144] The incubator 1 has a first side 17 and a second side 18. The first side 17 and the second side 18 are disposed opposite each other. The first side 17 is disposed opposite a first wall 24, and the second side 18 is disposed opposite a second wall 25. An air supply duct 211 is disposed within the first wall 24, and an air return duct 213 is disposed within the second wall 25. This allows hot air to enter the incubator 1 through the first side 17 and exit through the second side 18. The hot air can pass through the incubator 1, improving temperature uniformity within the incubator 1.

[0145] To facilitate the formation of the return air duct 213, the second box wall 25 may optionally include a second inner plate 251 and a second outer plate 252 spaced apart from each other, with the return air duct 213 formed between the second inner plate 251 and the second outer plate 252. The structure of the second box wall 25 can form the return air duct 213 and also form the box wall of the box body 2.

[0146] The return air duct 213 has a return air inlet 215 and a return air outlet 214. The return air inlet 215 communicates with the second side 18 of the incubator 1. Air flowing out of the incubator 1 flows out of the second side 18, then enters the return air duct 213 through the return air inlet 215, and finally re-enters the heating mechanism 3 through the return air outlet 214. Preferably, the return air inlet 215 is located on the inner side of the second chamber wall 25. More specifically, the return air outlet 214 is located on the second inner panel 251.

[0147] As Figure 5a shown, for the convenience of forming the air supply duct 211, optionally, the first box wall 24 may include a first inner plate 241 and a first outer plate 242 which are arranged at intervals, and the air supply duct 211 is formed between the first inner plate 241 and the first outer plate 242. The structure of the first box wall 24 can not only form the air supply duct 211 but also form the box wall of the box body 2.

[0148] The air supply duct 211 has an air supply outlet end 212 and an air supply inlet end 216. Hot air flows into the air supply duct 211 from the heating mechanism 3 through the air supply inlet end 216 and flows out through the air supply outlet end 212. The air supply outlet end 212 is communicated with the first side 17 of the incubator 1, and then the hot air enters the incubator 1 from the first side 17. Preferably, the air supply outlet end 212 is opened on the inner side of the first box wall 24. More specifically, the air supply outlet end 212 is opened on the first inner plate 241. The air supply inlet end 216 is opened on the inner side of the first box wall 24. More specifically, the air supply outlet end 212 is opened on the first inner plate 241.

[0149] It can be understood that the air supply duct 211 is communicated with the heating mechanism 3 through the air supply inlet end 216, and the air return duct 213 is communicated with the heating mechanism 3 through the air return outlet end 214.

[0150] As Figure 3 and Figure 6 shown, the air return inlet end 215 may include a plurality of air outlet holes 2151 which are arranged at intervals. The air outlet holes 2151 can make the air entering the incubator 1 more evenly distributed and improve the temperature uniformity in the incubator 1.

[0151] As Figure 7 and Figure 8 shown, the air supply outlet end 212 may include a plurality of air inlet holes 2121 which are arranged at intervals. The air inlet holes 2121 can make the air in the incubator 1 flow out of the incubator 1 evenly and improve the temperature uniformity in the incubator 1.

[0152] As Figure 3 and Figure 7 shown, exemplarily, when a plurality of incubators 1 are arranged in the box body 2, the number of the air return inlet ends 215 is the same as the number of the incubators 1, the number of the air supply outlet ends 212 is the same as the number of the incubators 1, the air return inlet ends 215 are arranged in one-to-one correspondence with the incubators 1, and the air supply outlet ends 212 are arranged in one-to-one correspondence with the incubators 1. Of course, one incubator 1 may also be correspondingly provided with a plurality of air return inlet ends 215 and a plurality of air supply outlet ends 212.

[0153] As Figure 5aAs shown, the air guiding structure 21 may further include an air supply buffer portion 26 for buffering and / or making the hot air distribution uniform. The air supply duct 211 and the air supply outlet end 212 are communicated through the air supply buffer portion 26. That is, the air in the air supply duct 211 enters the air supply outlet end 212 after passing through the air supply buffer portion 26. The air supply buffer portion 26 slows down the wind speed entering the incubator 1 and can also guide the hot air to make the hot air distribution more uniform, so that the hot air entering the incubator 1 is evenly distributed.

[0154] Specifically, the air supply buffer portion 26 is arranged in the air supply duct 211. In this embodiment, the air supply buffer portion 26 is arranged between the first inner plate 241 and the first outer plate 242 to facilitate the hot air in the air supply duct 211 to enter the air supply buffer portion 26 and the hot air in the air supply buffer portion 26 to enter the incubator 1. The air supply buffer portion 26 is arranged adjacent to the air supply outlet end 212 so that the hot air in the air supply buffer portion 26 can enter the incubator 1.

[0155] As shown in FIG. 4 and Figure 7 As shown, the air guiding structure 21 may further include a return air buffer portion 27 for buffering air. The return air duct 213 and the return air inlet end 215 are communicated through the return air buffer portion 27. That is, the air in the return air duct 213 enters the return air inlet end 215 after passing through the return air buffer portion 27. The return air buffer portion 27 can slow down the outflow of the hot air from the incubator 1, so that the hot air and the sample can perform sufficient heat exchange. At the same time, it can also make the air in each part of the incubator 1 flow out of the incubator 1 evenly.

[0156] Specifically, the return air buffer portion 27 is arranged in the return air duct 213. In this embodiment, the return air buffer portion 27 is arranged between the second inner plate 251 and the second outer plate 252 to facilitate the air in the incubator 1 to enter the air supply buffer portion 26 and the air in the air supply buffer portion 26 to enter the return air duct. The return air buffer portion 27 is arranged adjacent to the return air inlet end 215 so that the air in the incubator 1 can enter the return air buffer portion 27.

[0157] As Figure 9 As shown, the air supply buffer portion 26 may include a first buffer frame 261. The first buffer frame 261 can allow hot air to flow through. The first buffer frame 261 is provided with a first opening 265 and an air supply opening 264. The air supply opening 264 is communicated with the air supply duct 211, so that the hot air in the air supply duct 211 enters the air supply buffer portion 26 through the air supply opening 264. The first opening 265 is arranged opposite to the air supply outlet end 212, so that the hot air enters the air supply outlet end 212 through the first opening 265. The cross-sectional area of the first opening 265 is larger than the cross-sectional area of the air supply opening 264, so that the speed of the hot air entering the incubator 1 can be slowed down.

[0158] The air supply buffer part 26 may further include an air supply pipe 267. The air supply pipe 267 is connected to the air supply port 264, and the air supply pipe 267 can guide air into the first buffer frame 261.

[0159] The first buffer frame 261 encloses a first buffer space 262. The first opening 265 and the air supply port 2 are both communicated with the first buffer space 262. The first buffer space 262 can make the air distribute more evenly in the first buffer frame 261.

[0160] The air supply buffer part 26 may further include a first partition part 263. A plurality of first ventilation holes 266 are formed in the first partition part 263. The first partition part 263 divides the first buffer space 262 into two communicating spaces. The first partition part 263 can further slow down the flow rate of air in the first buffer space 262 and further make the hot air distribute evenly. It can be understood that the first opening 265 is communicated with one of the spaces, and the air supply port 264 is communicated with the other space, so that the hot air passes through the first partition part 263.

[0161] As Figure 10 shown, the return air buffer part 27 may include a second buffer frame to 271. The second buffer frame 271 is provided with a second opening 275 and a return air port 274. The second opening 275 is disposed opposite to the return air inlet end 215, so that the air in the incubator 1 enters the second buffer frame 271 through the return air inlet end 215 and the second opening 275. The return air port 274 is communicated with the return air duct 213, so that the air in the second buffer frame 271 enters the return air duct 213 through the return air port 274. The cross-sectional area of the second opening 275 is larger than the cross-sectional area of the return air port 274, thereby slowing down the speed of the air in the incubator 1 entering the second buffer frame 271 and enabling the air in the second buffer frame 271 to flow quickly into the return air duct 213.

[0162] The return air buffer part 27 may further include a return air pipe 277. The return air pipe 277 is connected to the return air port 274, and the return air pipe 277 can guide air into the return air duct 213.

[0163] The second buffer frame 271 encloses a second buffer space 272. The second opening 275 and the return air port 274 are both communicated with the second buffer space 272. The second buffer space 272 can slow down the speed of the air in the incubator 1 entering the return air duct 213.

[0164] The return air buffer part 27 may further include a second partition part 273. A plurality of second ventilation holes 276 are formed in the second partition part 273. The second partition part 273 divides the second buffer space 272 into two communicating spaces. The second partition part 273 divides the second buffer space 272 into two communicating spaces, and the second partition part 273 can further slow down the speed of air flowing into the second buffer space 272. It can be understood that the second opening 275 communicates with one of the spaces, and the air return port 274 communicates with the other space, so that the hot air passes through the second partition part 273.

[0165] Exemplarily, the sample can be placed in the culture tube 100. An identification part such as a two-dimensional code or a bar code can be pasted on the culture tube 100. As Figure 7 shown, the sample culture detection system may further include a scanning module 6. The scanning module 6 is used to scan the identification part on the culture tube 100. After the scanning module 6 scans, it can indicate the tube placement position, and make the display of the detection result correspond to the sample one by one, so as to facilitate subsequent tube placement and tube taking operations, and facilitate the statistics of the detection results.

[0166] The sample culture detection system may further include a user terminal 8. The user terminal 8 is electrically connected to the incubation and culture device and the scanning module 6. In this embodiment, the user terminal 8 may include an input / output module and a control module. The input / output module may be a touch screen, and the touch screen is connected to the box body 2. The control module is electrically connected to the input / output module, the incubation and culture device, and the scanning module 6. In other embodiments, the user terminal 8 may also be a mobile phone, a tablet computer, a computer, etc. The user terminal 8 and the incubation and culture device and the scanning module 6 may be wirelessly or wiredly connected through WiFi, Bluetooth, etc.

[0167] The control module may be a centralized or distributed controller. For example, the controller may be a single microcontroller, or may be composed of multiple distributed microcontrollers. A control program may run in the microcontroller, and then control the above-mentioned various components to realize their functions.

[0168] Exemplarily, the touch screen can display the temperature in each incubator 1, and can also adjust the target temperature in the incubator 1 by clicking on the touch screen, and then control the opening and closing of the fresh air mechanism 4 and the power adjustment of the heating mechanism 3 through the control module to adjust the temperature. After the scanning module 6 performs a scanning operation on the culture tube 100, the tube placement position can be displayed on the touch screen, so as to facilitate subsequent tube placement and tube taking operations. The user terminal 8 may also be electrically connected to a printer, thereby facilitating the user to print a report. The user terminal 8 also facilitates the real-time display of the detection result, improving the convenience for the user.

[0169] Optionally, the user terminal 8 is a terminal based on the Android system, so that the interface of the touch screen is more concise and clear, and the operation is simple and convenient.

[0170] As shown in Figure 11 FIG. 1, the incubator 1 may include a first housing 15 that is capable of accommodating a culture tube 100 for holding a sample, and at least a portion of the culture tube 100 that holds the sample is located within the first housing 15, thereby heating the sample.

[0171] The first housing 15 may include a first side wall 151 and a second side wall 152 that are oppositely disposed. The air heated by the heating mechanism 3 can enter the first housing 15 through the first side wall 151, and the air within the first housing 15 can flow out through the second side wall 152, so that the hot air passes through the entire interior of the first housing 15. Optionally, the guiding structure 21 communicates with the incubator 1 through the first side wall 151 and the second side wall 152. Specifically, the first side wall 151 is located on the first side 17 of the incubator 1, and the second side wall 152 is located on the second side 18 of the incubator 1.

[0172] Second air holes 1521 are formed on both the first side wall 151 and the second side wall 152, and the guiding structure 21 communicates with the incubator 1 through the second air holes 1521.

[0173] As shown in Figure 12 FIG. 2, the first housing 15 may further include a bottom wall 153. The bottom wall 153 may be connected to the lower ends of the first side wall 151 and the second side wall 152. The bottom wall 153 is provided with a plurality of first air holes 1531. Exemplarily, there is a gap between the bottom wall 153 and a partition 28 located below it. The first air holes 1531 may allow the air inside the incubator 1 to communicate with the air outside the incubator 1 (it can be understood that this air is also located within the box body 2), so that the temperature within the entire box body 2 tends to be uniform, and further helps to keep the temperature within the incubator 1 consistent.

[0174] As shown in Figure 13 - Figure 15a FIG. 3, the first housing 15 may be provided with a plurality of hole positions 1541 for inserting the culture tube 100. Optionally, the first housing 15 may further include a top wall 154. The top wall 154 may be connected to the upper ends of the first side wall 151 and the second side wall

[0175] Optionally, the incubator 1 may further include a positioning plate 19 disposed below the top wall 154. The positioning plate 19 is provided with positioning holes 191. The culture tube 100 can be inserted into the positioning holes 191. Further, a position near the lower end of the culture tube 100 can be inserted into the positioning holes 191, and the lower end of the culture tube 100 passes through the positioning holes 191 and is located below the positioning plate 19. The positioning plate 19 can improve the placement stability of the culture tube 100.

[0176] Optionally, the top wall 154 may include an upper top plate 1542 and a lower top plate 1543, and the upper top plate 1542 is located outside the lower top plate 1543. The incubator 1 may further include a first heating unit 16 for heating a sample in the incubator 1. Exemplarily, the first heating unit 16 is clamped between the lower top plate 1543 and the upper top plate 1542, so as to facilitate fixing the first heating unit 16. Holes may be formed in the first heating unit 16, the upper top plate 1542 and the lower top plate 1543, and the holes in the first heating unit 16, the upper top plate 1542 and the lower top plate 1543 are arranged opposite to each other to form a hole position 1541.

[0177] In this embodiment, the top wall 154 may be provided with 400 hole positions 1541. Of course, the number of hole positions 1541 is not limited to this, so as to meet the high-throughput detection target. Optionally, a plurality of hole positions 1541 are arranged in an array along a first direction X and a second direction Y to improve the detection throughput. Exemplarily, the first direction X and the second direction Y are in a horizontal plane, that is, the first direction X, the second direction Y and the vertical direction Z are perpendicular to each other in pairs. In other alternative embodiments, the first direction X and the second direction Y may also be in other planes.

[0178] The first heating unit 16 can independently adjust the temperature in the corresponding incubator 1, so as to accurately compensate the temperature in the incubator 1. Optionally, the first heating unit 16 may be in a sheet shape. Exemplarily, heating is performed by the heating mechanism 3 and the first heating unit 16 in the initial rising stage. When the temperature in a single incubator 1 is lower than the target temperature, the corresponding first heating unit 16 can be independently controlled to heat the sample in the incubator 1, so as to further increase the heating rate and improve the detection efficiency.

[0179] Optionally, the incubator 1 may further include a second temperature detection unit (not shown in the figure) for detecting the temperature in the incubator 1. The second temperature detection unit may be a temperature sensor or the like, and the first heating unit 16 may adjust the temperature in the incubator 1 according to the temperature detected by the second temperature detection unit.

[0180] Optionally, the control module may also be electrically connected to the first heating unit 16, and the first temperature detection unit and the second temperature detection unit may also be connected to the control module. The first temperature detection unit and the second temperature detection unit transmit the detected temperature values to the control module, and the control module controls the first heating unit 16 and the heating mechanism 3 according to the received temperature values.

[0181] Exemplarily, the control module may also be electrically connected to the fresh air mechanism 4 to control the start and stop of the fresh air mechanism 4.

[0182] When the temperature in the incubator 1 is relatively low, the control module can control the fresh air mechanism 4 to close, so that the outside air cannot enter the box body 2. The control module controls the first heating unit 16 and the heating mechanism 3 to heat the hot air in the incubator 1, and the diversion structure 21 conveys the air heated by the heating mechanism 3 into the incubator 1, so that the temperature in the incubator 1 can reach the target temperature as soon as possible; when the temperature in the incubator 1 needs to be lowered, the control module can control the fresh air mechanism 4 to open, and the control module controls the first heating unit 16 and the heating mechanism 3 to stop working or reduce the power to lower the temperature in the incubator 1.

[0183] Optionally, the user terminal 8 is electrically connected to the incubation and cultivation device and the fluorescence detection device 10. Exemplarily, the control module is electrically connected to the fluorescence detection device 10, so that the fluorescence detection device 10 can transmit the detected data to the control module, and the control module transmits the data to the touch screen for display, so that the user can check the detection results at any time. The control module can also control the fluorescence detection device 10 to detect a specified sample.

[0184] Exemplarily, the bottom of the culture tube 100 is transparent, and the fluorescence detection device 10 detects the sample through the bottom of the culture tube 100. In this way, the opening of the culture tube 100 can be covered with a cover body, so that the air does not directly contact the sample and prevents the sample from being contaminated.

[0185] In this embodiment, the fluorescence detection device 10 is arranged in the incubator 1. Preferably, the fluorescence detection device 10 is located below the positioning plate 19 to perform fluorescence detection on the sample through the bottom of the culture tube 100. The incubation and cultivation device of the sample culture and detection system can accurately control the temperature in the large-capacity box body 2 and ensure its temperature accuracy and uniformity. At the same time, the incubation and cultivation device is internally equipped with a fluorescence detection device 10 for synchronous detection, improving the detection efficiency.

[0186] Optionally, the fluorescence detection device 10 is provided in one-to-one correspondence with the incubator 1, so as to facilitate the detection of samples in each incubator 1.

[0187] As Figure 16a shown, the fluorescence detection device 10 may include a fluorescence detection module 11. The fluorescence detection module 11 is used to detect the sample. Exemplarily, the fluorescence detection module 11 may include an excitation light source and a photoelectric sensor. The excitation light source emits light to excite the fluorescent substance at the bottom of the culture tube 100. The fluorescent substance generates fluorescence and is received by the photoelectric sensor, and finally the fluorescence signal analysis is realized through the control mechanism. The fluorescence detection module 11 may be an existing module that can detect fluorescence signals and will not be specifically introduced here.

[0188] As Figure 16a - Figure 17aAs shown, the fluorescence detection device 10 may further include a driving mechanism 12. The driving mechanism 12 is configured to drive the fluorescence detection module 11 to move, so that the fluorescence detection module 11 can detect a plurality of samples located at different positions respectively. Optionally, the driving mechanism 12 is connected to the first housing 15.

[0189] The driving mechanism 12 can move the fluorescence detection module 11 to different positions, so as to detect samples at different positions. That is, one fluorescence detection module 11 can correspond to multiple samples. The fluorescence detection device 10 has a large detection throughput, low equipment cost, and small equipment volume, solving the problem in the prior art that one fluorescence detection module 11 needs to correspond to one sample, resulting in a small detection throughput and high equipment cost.

[0190] Furthermore, the driving mechanism 12 is configured to drive the fluorescence detection module 11 to reciprocate along the first direction X and the second direction Y. Exemplarily, the driving mechanism 12 drives the fluorescence detection module 11 to move along the second direction Y to detect a row of samples placed along the second direction Y respectively. Subsequently, the driving mechanism 12 drives the fluorescence detection module 11 to move along the first direction X, so that the fluorescence detection module 11 moves to the next row of samples. The above actions are repeated until the fluorescence detection module 11 has detected all the samples.

[0191] As Figure 16a - Figure 17b shown, the driving mechanism 12 may include a first driving component 121 and a second driving component 122. The first driving component 121 is connected to the second driving component 122 to drive the second driving component 122 to move along the first direction X. Subsequently, the fluorescence detection module 11 completes corresponding detection actions at the corresponding positions. The second driving component 122 is connected to the fluorescence detection module 11 to drive the fluorescence detection module 11 to move along the second direction Y.

[0192] During detection, the second driving component 122 drives the fluorescence detection module 11 to move along the second direction Y to detect a plurality of samples placed along the second direction Y (the plurality of samples are arranged in a row along the second direction Y) respectively. Subsequently, the first driving component 121 drives the fluorescence detection module 11 and the second driving component 122 to move along the first direction X, so that the fluorescence detection module 11 moves to the next row of samples. The above actions are repeated until the fluorescence detection module 11 has detected all the samples.

[0193] Optionally, the first drive assembly 121 may include a first driving member 1211, a first belt 1214, a first driving pulley 1212, and a first driven pulley 1213. The first driving member 1211 is connected to a side wall of the first housing 15 and may be a motor. The first driving pulley 1212 and the first driven pulley 1213 are spaced apart along a first direction X and rotatably connected to the bottom wall 153 of the first housing 15. The first driving pulley 1212 is connected to the first driving member 1211 so that the first driving member 1211 drives the first driving pulley 1212 to rotate. The first belt 1214 is sleeved over the first driving pulley 1212 and the first driven pulley 1213.

[0194] The first driving member 1211 works to drive the first active pulley 1212 to rotate, thereby driving the first belt 1214 sleeved outside the first active pulley 1212 to rotate. The second driving component 122 is connected to the first belt 1214, and then the second driving component 122 moves along the first direction X driven by the first belt 1214.

[0195] The second drive assembly 122 may include a mounting portion 1221, a second belt 1224, a second driving pulley 1222, and a second driven pulley 1223. The mounting portion 1221 is connected to the first drive assembly 121. Specifically, the mounting portion 1221 is connected to the first belt 1214. A second driving member, which may be a motor, is connected to the mounting portion 1221. The second driving pulley 1222 and the second driven pulley 1223 are spaced apart along the second direction Y and are rotationally connected to the mounting portion 1221. The second driving pulley 1222 is connected to the second driving member so that the second driving member drives the second driving pulley 1222 to rotate. The second belt 1224 is sleeved over the second driving pulley 1222 and the second driven pulley 1223.

[0196] The second driving member works to drive the second active pulley 1222 to rotate, thereby driving the second belt 1224 mounted outside the second active pulley 1222 to rotate. The fluorescence detection module 11 is connected to the second belt 1224, and then the fluorescence detection module 11 is driven to move along the second direction Y under the rotation of the second belt 1224.

[0197] like Figure 16c As shown, the second drive assembly 122 may optionally further include at least two tensioning pulleys 1225. The tensioning pulleys 1225 are disposed between the second driving pulley 1222 and the second driven pulley 1223 and are used to tension the second belt 1224, thereby improving the belt's motion accuracy. In this embodiment, there are three tensioning pulleys 1225, and at least two of the tensioning pulleys 1225 may be spaced apart in the vertical direction Z to tension the second belt 1224.

[0198] likeFigure 16a , Figure 17a and Figure 17b As shown in and

[0199] , the drive mechanism 12 may further include a first guiding assembly 123. The first guiding assembly 123 is configured to guide the second driving assembly 122. That is, when the second driving assembly 122 moves along the first direction X, the first guiding assembly 123 can guide the second driving assembly 122 to improve the movement stability of the first guiding assembly 123.

[0199] Optionally, the first guiding assembly 123 may include a first guide rail 1231 and a first guiding block 1232. The first guide rail 1231 extends along the first direction X. Exemplarily, the first guide rail 1231 is connected to the bottom wall 153 of the first housing 15. The first guiding block 1232 is slidably disposed on the first guide rail 1231, and the second driving assembly 122 is connected to the first guiding block 1232. Specifically, the mounting portion 1221 of the second driving assembly 122 is connected to the first guiding block 1232. The number of the first guide rails 1231 may be one or two. When the number of the first guide rails 1231 is two, the two first guide rails 1231 are arranged in parallel.

[0200] As shown in Figure 16a , Figure 16b and Figure 17a , optionally, the drive mechanism 12 may further include a second guiding assembly 124. The second guiding assembly 124 is configured to guide the fluorescence detection module 11 to improve the movement stability of the fluorescence detection module. Figure 16a , Figure 16b and Figure 17a Exemplarily, the second guiding assembly 124 may include a second guide rail 1241 and a second guiding block 1242. The second guide rail 1241 extends along the second direction Y. Optionally, the second guide rail 1241 is connected to the mounting portion 1221. The second guiding block 1242 is slidably disposed on the second guide rail 1241, and the fluorescence detection module 11 is connected to the second guiding block 1242.

[0201] As shown in and

[0202] , optionally, the second guiding assembly 124 may include a second guide rail 1241 and a second guiding block 1242. The second guide rail 1241 extends along the second direction Y. Optionally, the second guide rail 1241 is connected to the mounting portion 1221. The second guiding block 1242 is slidably disposed on the second guide rail 1241, and the fluorescence detection module 11 is connected to the second guiding block 1242.

[0202] As Figure 17aAs shown, optionally, the fluorescence detection device 10 may further include an in-position detection mechanism 13 connected to the driving mechanism 12. The in-position detection mechanism 13 is used to detect whether a sample exists at a plurality of preset positions, and the preset positions are used to place samples. In this embodiment, the preset position refers to the hole position 1541, that is, the in-position detection mechanism 13 is used to detect whether there is a sample on the hole position 1541. If there is a sample on the hole position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the hole position 1541, the fluorescence detection module 11 does not need to detect the sample at this hole position 1541. The in-position detection mechanism 13 can pre-identify whether a sample exists. The pre-identification function of the in-position detection mechanism 13 is integrated with the fluorescence detection function. As a new application method, it can effectively improve the detection efficiency and subsequent data processing efficiency, especially in high-throughput detection, and can effectively improve the subsequent data processing efficiency.

[0203] Optionally, the in-position detection mechanism 13 may include a plurality of in-position detection modules 131. The in-position detection module 131 may be a photoelectric sensor or the like. Exemplarily, the plurality of in-position detection modules 131 are sequentially arranged along the second direction Y. Further, the plurality of in-position detection modules 131 correspond one-to-one to a plurality of hole positions 1541 arranged in a row along the second direction Y, and the in-position detection mechanism 13 can detect whether there is a sample on a row of hole positions 1541 arranged along the second direction Y at one time. This can reduce the usage frequency of each in-position detection module 131 and extend the usage time of the in-position detection module 131.

[0204] The driving mechanism 12 is used to drive the plurality of in-position detection modules 131 to move along the first direction X, so that the in-position detection mechanism 13 can detect whether there is a sample on all the hole positions 1541. At this time, the first driving component 121 is connected to the in-position detection mechanism 13 to drive the in-position detection module 131 to move along the first direction X. Further, the in-position detection mechanism 13 is connected to the first belt 1214. For example, the in-position detection mechanism 13 is connected to the mounting portion 1221, and the in-position detection mechanism 13 is connected to the first belt 1214 through the mounting portion 1221. At this time, the in-position detection mechanism 13 is also connected to the first guiding block 1232 through the mounting portion 1221. Therefore, at this time, the first guiding component 123 can also guide the in-position detection mechanism 13.

[0205] Optionally, the in-position detection mechanism 13 may further include an in-position detection mounting plate 132. The in-position detection mounting plate 132 is connected to the driving mechanism 12, and the in-position detection module 131 is connected to the in-position detection mounting plate 132. When the number of in-position detection modules 131 is multiple, the in-position detection mounting plate 132 is connected to the mounting portion 1221 to move synchronously with the mounting portion 1221.

[0206] In other alternative embodiments, the in-position detection mechanism 13 may further include an in-position detection module 131 to reduce the cost of the fluorescence detection device 10.

[0207] The following details the situation when the in-position detection mechanism 13 may include an in-position detection module 131:

[0208] At this time, the second driving component 122 is connected to the in-position detection module 131 to drive the in-position detection module 131 to move along the second direction Y. Specifically, the in-position detection module 131 is connected to the second belt 1224. Among them, the in-position detection module 131 may be connected to the second belt 1224 through the in-position detection mounting plate 132. Since the first driving component 121 is connected to the second driving component 122 and drives the second driving component 122 to move along the first direction, and the in-position detection module 131 is connected to the second driving component 122, therefore, the first driving component 121 also drives the in-position detection module 131 to move along the first direction, realizing the movement of the in-position detection module 131 along the first direction and the second direction, and further detecting whether there are samples on all the hole positions 1541.

[0209] The second guiding component 124 can guide the in-position detection mechanism 13 to enable the in-position detection mechanism 13 to move stably in the second direction Y. Specifically, the second guiding block 1242 is connected to the in-position detection mechanism 13. Further, the in-position detection mechanism 13 is connected to the second guiding block 1242 through the in-position detection mounting plate 132.

[0210] During the detection process, a round of detection is performed every preset time, such as once every hour, or it can start the detection at the whole hour, such as starting a round of detection at 1 o'clock sharp, 2 o'clock sharp... and other time points.

[0211] The high-speed fluorescence device provided in this embodiment has the characteristics of high throughput, fast speed, and low cost, and the detection efficiency is not lower than that of the array-type fluorescence detection (that is, the form where multiple fluorescence detection modules correspond to multiple samples one by one). Moreover, since the high-speed fluorescence device provided in this embodiment is a single fluorescence detection module 11, the cost is lower.

[0212] Each incubator 1 in the incubation and culture device provided in this embodiment can incubate 400 samples. Setting three incubators 1 can meet the requirement of incubating 1200 samples simultaneously. The incubation and culture device can accurately control the temperature in the large-capacity box body 2, ensure the temperature accuracy and uniformity, and at the same time facilitate the synchronous detection by the fluorescence detection device 10 embedded in the incubation and culture device, improving the detection efficiency. The incubation and culture device provided in this embodiment can accurately control the compensation temperature of the first heating unit 16. At the same time, in cooperation with the diversion structure 21, the dynamic balance of the temperature in the box body 2 can be achieved, so that the temperature in the incubator 1 reaches the target temperature ±1.5°C. In this embodiment, the fresh air mechanism 4 and the diversion structure 21 perform rapid temperature control, so as to achieve the temperature of each hole position 1541 in the incubator 1 reaching the incubation temperature condition and meet the requirement of rapid incubation and culture.

[0213] When using the sample culture and detection system provided in this embodiment, since the in-position detection mechanism 13 can detect whether there is a sample on the hole position 1541 before fluorescence detection. If there is a sample on the hole position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the hole position 1541, the fluorescence detection module 11 does not need to detect the sample at this hole position 1541. Thus, the detection efficiency and the subsequent data processing efficiency can be effectively improved. At the same time, the fluorescence detection module 11 can move, so that the samples can be detected separately quickly. For the above reasons, the high-speed fluorescence detection device provided in this embodiment only takes about 10 minutes to complete the test of 400 hole positions, greatly improving the detection efficiency. In addition, since this embodiment is provided with three layers of incubators 1 (of course, it can also be not limited to three layers), it can meet the simultaneous detection of 1200 samples, further improving the detection efficiency.

[0214] As Figure 18 shown, this embodiment also provides a sample culture and detection method, and the above sample culture and detection system can execute the sample culture and detection method. The sample culture and detection method may include:

[0215] S1. The incubation and culture device incubates the samples located therein; optionally, the samples can be incubated at 37°C, and the incubation duration can be 24 hours, but the incubation temperature and time of the samples are not limited thereto.

[0216] S2. The fluorescence detection device 10 detects the samples. It can be understood that the above two steps S1 and S2 do not represent the order of sequence. The fluorescence detection device 10 can detect during the sample incubation process.

[0217] In the sample culture and detection method provided in this embodiment, while the samples are incubated, the fluorescence detection device 10 can detect the samples, thus greatly improving the detection efficiency.

[0218] When the sample culture detection system can include the scanning module 6, before the user puts the sample into the incubation and culture device, it can further include step S3, where the scanning module 6 scans and identifies the culture tube 100 containing the sample. By identifying the identification part on the culture tube 100 through the scanning module 6, it is convenient for subsequent tube placement and removal operations, as well as result statistics.

[0219] Step S1 can include: heating the environment inside the box body 2 through the heating mechanism 3 to incubate the sample located therein.

[0220] When the number of incubators 1 is multiple, step S1 can include: heating the sample through the first heating unit 16 and the heating mechanism 3 to quickly reach the target temperature. Exemplarily, when in the initial rising stage, heating is carried out jointly by the heating mechanism 3 and the first heating unit 16 to achieve rapid temperature rise.

[0221] When the number of incubators 1 is multiple, step S1 can also include: compensating the temperature inside the incubator 1 through the first heating unit 16 to make the temperature of the sample reach the target temperature. For example, when the temperature inside a certain incubator 1 is lower than the target temperature, the first heating unit 16 can be precisely controlled for temperature compensation to keep the temperature inside the entire box body 2 within the range of the target temperature ±1.5°C.

[0222] When the temperature inside the incubator 1 is higher than the target temperature, air enters the incubation and culture device through the fresh air mechanism 4. The temperature of the outside air is lower than the temperature of the air inside the box body 2. Therefore, introducing outside air into the box body 2 can quickly reduce the temperature inside the incubator 1 to achieve rapid temperature control. The fresh air mechanism 4 can filter the air to keep the inside of the incubation and culture device clean.

[0223] Exemplarily, when the temperature inside the incubator 1 is lower than the target temperature, the heating mechanism 3 operates at full load. Exemplarily, both the second heating unit and the air driving member 31 operate at full load; when the temperature inside the incubator 1 is higher than the target temperature, the heating mechanism 3 operates at light load. Exemplarily, both the second heating unit and the air driving member 31 operate at light load, and the fresh air mechanism 4 introduces outside air into the box body 2 and / or the heating mechanism 3, and then sends the cooled air into the incubator 1 through the diversion structure 21 to cool the incubator 1.

[0224] As Figure 19 shown, step S2, where the fluorescence detection device 10 detects the sample can include:

[0225] S21. In-situ detection: The in-situ detection mechanism 13 detects whether there is a sample at the preset position (i.e., the hole position 1541); specifically, the driving mechanism 12 drives the in-situ detection mechanism 13 to move into position, and the in-situ detection mechanism 13 detects whether there is a sample at the preset position.

[0226] S22. Fluorescence detection: If there is a sample at the preset position, the fluorescence detection module 11 performs detection. Specifically, the driving mechanism 12 drives the fluorescence detection module 11 to move into place, and the fluorescence detection module 11 detects the sample.

[0227] In this embodiment, before performing fluorescence detection, it is first detected whether there is a sample on the well position 1541. If there is a sample on the well position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the well position 1541, the fluorescence detection module 11 does not perform detection at this well position 1541. The in-situ detection and fluorescence detection are integrated, which can effectively improve the detection efficiency and the subsequent data processing efficiency. Especially in high-throughput detection, it can effectively improve the subsequent data processing efficiency. Through the movement of the in-situ detection mechanism 13 and the fluorescence detection module 11, the sample culture and detection method can adapt to high-throughput detection.

[0228] Optionally, step S2 may further include:

[0229] S23. Return to the initial position: The in-situ detection mechanism 13 and the fluorescence detection module 11 move to the initial position. It is possible to execute the above steps before step S21 in-situ detection and step S22 fluorescence detection, so that each detection starts from the initial position, which is convenient for subsequent data processing. Optionally, the initial position may be the first well position 1541 at the corner.

[0230] Optionally, before step S21 in-situ detection and step S22 fluorescence detection, it may further include:

[0231] S24. Instrument self-check: The in-situ detection mechanism 13 and the fluorescence detection module 11 move to the self-check position and perform self-check. Optionally, the self-check position may be the initial position of the in-situ detection mechanism 13 and the fluorescence detection module 11, so as to perform detection quickly after self-check.

[0232] A calibration tube and an empty space (that is, the well position 1541 is empty and no culture tube 100 is placed) may be set on the well position 1541 directly opposite to the initial position. When the in-situ detection mechanism 13 and the fluorescence detection module 11 are located at the initial position, the fluorescence detection module 11 performs self-check and automatically sets the reference signal value by detecting the calibration tube. The in-situ detection mechanism 13 performs self-check and automatically sets the reference signal value at the empty space.

[0233] Optionally, the number of preset positions is multiple, that is, there are multiple hole positions 1541, and the multiple preset positions are arranged in an array. The in-position detection can include the in-position detection mechanism 13 detecting a row of preset positions, which can reduce the usage frequency of a single in-position detection module 131 and improve the service life of a single in-position detection module 131. After the in-position detection mechanism 13 detects a row of hole positions 1541, the fluorescence detection module 11 sequentially detects the samples on the hole positions 1541 where the samples are placed.

[0234] After the fluorescence detection module 11 detects the samples at all preset positions, after a preset time, the samples at all preset positions are detected again. Optionally, the preset time can be 1 hour, that is, every 1 hour, the fluorescence detection module 11 performs a round of data acquisition on all samples and sends the detection results to the control mechanism for arithmetic analysis until the sample incubation is completed and the detection of the samples stops.

[0235] Optionally, the culture tube 100 can be placed or removed during the detection process. The fluorescence detection device 10 detecting the samples can further include:

[0236] After the user performs the operation of placing and / or removing the tube, the position detection mechanism detects the position of placing and / or removing the tube. If the culture tube 100 is not placed or removed correctly, a prompt is issued. In this way, it is confirmed whether the user places / removes the culture tube 100 correctly to avoid incorrect detection data.

[0237] Although the present utility model has been described in detail above with general descriptions, specific embodiments and experiments, based on the present utility model, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present utility model all fall within the scope of protection required by the present utility model.

Claims

1. A sample culture detection system, characterized in that, Comprising: An incubation and culture device for incubating samples; A fluorescence detection device (10) for detecting samples within the incubation and culture device; The incubation and culture device includes: A box body (2); An incubator (1) for accommodating samples and capable of being disposed within the box body (2); A heating mechanism (3) disposed within the box body (2), the heating mechanism (3) being capable of heating the samples within the incubator (1).

2. The sample culture detection system according to claim 1, wherein, The box body (2) includes a flow guiding structure (21), the flow guiding structure (21) enabling air to circulate between the heating mechanism (3) and the incubator (1).

3. The sample culture detection system according to claim 2, characterized in that, The flow guiding structure (21) includes an air supply duct (211) and a return air duct (213), the heating mechanism (3), the air supply duct (211), the incubator (1), and the return air duct (213) being sequentially connected to form an air circulation loop.

4. The sample culture detection system according to claim 3, wherein, The air supply outlet end (212) of the air supply duct (211) is connected to the first side (17) of the incubator (1), the return air inlet end (215) of the return air duct (213) is connected to the second side (18) of the incubator (1), and the first side (17) and the second side (18) are oppositely disposed.

5. The sample culture detection system according to claim 4, wherein, The box body (2) further includes a first box wall (24) and a second box wall (25), the air supply duct (211) being disposed within the first box wall (24), and the return air duct (213) being disposed within the second box wall (25).

6. The sample culture detection system according to claim 5, characterized in that, The first box wall (24) and the second box wall (25) are oppositely disposed; and / or The incubator (1) is disposed between the first box wall (24) and the second box wall (25), and the first box wall (24) is oppositely disposed to the first side (17) of the incubator (1), and the second side (18) of the incubator (1) is oppositely disposed to the second box wall (25).

7. The sample culture and detection system according to any one of claims 4-6, characterized in that, The air supply outlet end (212) includes a plurality of spaced-apart air inlet holes (2121); and / or, the return air inlet end (215) includes a plurality of spaced-apart air outlet holes (2151).

8. The sample culture and detection system according to any one of claims 4-6, characterized in that, The flow guiding structure (21) further includes an air supply buffer portion (26) for buffering hot air and / or making the hot air distribution uniform, the air supply duct (211) and the air supply outlet end (212) being connected through the air supply buffer portion (26); and / or The flow guiding structure (21) further includes a return air buffer portion (27) for buffering air, the return air duct (213) and the return air inlet end (215) being connected through the return air buffer portion (27).

9. The sample culture and detection system according to any one of claims 4-6, characterized in that, At least one air supply outlet end (212) is correspondingly provided for one incubator (1); and / or At least one return air inlet end (215) is correspondingly provided for one incubator (1).

10. The sample culture and detection system according to any one of claims 2 or 3, characterized in that, The heating mechanism (3) includes an air driving member (31), the air driving member (31) being used for driving air to flow between the heating mechanism (3) and the incubator (1).

11. The sample culture and detection system according to claim 2, characterized in that, The box body (2) has a receiving space (23) near the bottom (22) of the box, the heating mechanism (3) is arranged in the receiving space (23), and the receiving space (23) communicates with the diversion structure (21).

12. The sample culture detection system according to claim 1, wherein The incubator (1) includes a first housing (15), the first housing (15) can be provided with a culture tube (100) for accommodating a sample, and at least the part of the culture tube (100) for accommodating the sample is located inside the first housing (15).

13. The sample culture and detection system according to claim 12, characterized in that, The first housing (15) is provided with a plurality of holes (1541) for inserting the culture tube (100).

14. The sample culture and detection system according to claim 13, wherein, The first housing (15) includes a top wall (154) and a positioning plate (19) arranged on the lower side of the top wall (154), the holes (1541) are opened on the top wall (154), the positioning plate (19) is provided with positioning holes (191), and the culture tube (100) can be inserted into the positioning holes (191).

15. The sample culture and detection system according to any one of claims 12-14, characterized in that, The incubator (1) further includes a calibration tube arranged inside the first housing (15), and the calibration tube is used to calibrate the fluorescence detection device (10).

16. The sample culture and detection system according to any one of claims 12-14, characterized in that, The first housing (15) includes a first side wall (151) and a second side wall (152) arranged opposite to each other. The air heated by the heating mechanism (3) can enter the first housing (15) through the first side wall (151), and the air inside the first housing (15) can flow out through the second side wall (152).

17. The sample culture detection system according to claim 16, wherein, The first housing (15) further includes a bottom wall (153) connected to the first side wall (151) and the second side wall (152), and the bottom wall (153) is provided with a plurality of first air holes (1531); and / or The second air holes (1,521) are opened on both the first side wall (151) and / or the second side wall (152).

18. The sample culture and detection system according to any one of claims 1-6, 12-14, characterized in that, The incubation and culture device further includes a fresh air mechanism (4) connected to the box body (2), the fresh air mechanism (4) is used for filtering air, and the outside air enters the box body (2) and / or the heating mechanism (3) through the fresh air mechanism (4).

19. The sample culture and detection system according to any one of claims 1-6 and 12-14, characterized in that The heating mechanism (3) includes a second housing (32) and a second heating unit, and the second heating unit is arranged inside the second housing (32).

20. The sample culture and detection system according to claim 19, wherein The heating mechanism (3) further includes a first temperature detection unit and / or a temperature protection unit arranged inside the second housing (32).

21. The sample culture and detection system according to any one of claims 1-6, characterized in that, The incubator (1) further includes a second temperature detection unit for detecting the temperature inside the incubator (1).

22. The sample culture and detection system according to any one of claims 1-6 and 12-14, wherein The number of the incubators (1) is one or more.

23. The sample culture and detection system according to claim 22, wherein The box body (2) further includes a partition (28), and the partition (s) (28) is / are used for separating two adjacent incubators (1).

24. The sample culture and detection system according to any one of claims 1-6, 12-14, characterized in that, The incubator (1) further includes a first heating unit (16) for heating the sample inside the incubator (1).

25. The sample culture and detection system according to any one of claims 1-6 and 12-14, characterized in that, The fluorescence detection device (10) performs fluorescence detection through the bottom of the culture tube (100).

26. The sample culture and detection system according to claim 1, wherein The fluorescence detection device (10) includes a fluorescence detection module (11) and a driving mechanism (12). The driving mechanism (12) is configured to drive the fluorescence detection module (11) to move, so that the fluorescence detection module (11) can detect a plurality of samples located at different positions in the incubator (1) respectively.

27. The sample culture detection system according to claim 26, wherein, The driving mechanism (12) is configured to drive the fluorescence detection module (11) to reciprocate along a first direction and a second direction, and the first direction and the second direction are perpendicular to each other.

28. The sample culture and detection system according to claim 26, characterized in that, The fluorescence detection device (10) further includes an in-position detection mechanism (13) connected to the driving mechanism (12). The in-position detection mechanism (13) is configured to detect whether there are samples at a plurality of preset positions, and the preset positions are used for placing samples.

29. The sample culture and detection system according to claim 28, wherein, The in-position detection mechanism (13) includes one or more in-position detection modules (131).

30. The sample culture and detection system according to claim 29, wherein, When the number of the in-position detection modules (131) is multiple, the multiple in-position detection modules (131) are arranged in sequence along the second direction, and the driving mechanism (12) is configured to drive the multiple in-position detection modules (131) to move along the first direction.

31. The sample culture and detection system according to any one of claims 1-6, 12-14, and 26-28, characterized in that, The multiple samples in the incubator (1) are arranged in an array along the first direction and the second direction.

32. The sample culture detection system according to any one of claims 1-6, 12-14, and 26-28, characterized in that, The sample culture detection system further includes a scanning module (6), and the scanning module (6) is configured to scan the identification part on the culture tube (100).

33. The sample culture and detection system according to claim 32, wherein The sample culture detection system further includes a user terminal (8), and the user terminal (8) is electrically connected to the incubation and culture device, the fluorescence detection device (10), and the scanning module (6).

34. The sample culture and detection system according to claim 33, characterized in that, The user terminal (8) includes an input / output module and a control module, and the control module is electrically connected to the input / output module, the incubation and culture device, the fluorescence detection device (1), and the scanning module (6).

35. The sample culture and detection system according to claim 33 or 34, characterized in that, The user terminal (8) is a terminal based on the Android system.

36. The sample culture detection system according to claim 27, wherein The first direction and the second direction are located in a horizontal plane.

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