Sample module capable of transversely feeding and nucleic acid extraction and amplification analyzer
By integrating the sample tube, Tip head assembly and reagent cartridge in the same direction, the problem of low integration in the prior art is solved, and the structure simplification and control logic optimization of the nucleic acid extraction amplification analyzer is achieved, which reduces costs.
Patent Information
- Application Number
- CN202421653896.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-12
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-07-12
AI Technical Summary
In the existing fully automatic nucleic acid extraction and amplification analyzers, the integration of storage modules such as sample tubes, Tip head components and reagent cartridges is not high, resulting in the need of pipetting pump components to move in multiple directions between each storage module, resulting in complex instrument structure and control.
A sample module that can be fed horizontally is designed. The sample tube, Tip head assembly and reagent cartridge are arranged in a verbatim direction and integrated on the same bracket. The pipetting pump only needs to be lifted and moved to realize the pipetting of the Tip head, suction and spit sample liquid and the reagent cartridge.
The structure and control logic of the analyzer are simplified, design and manufacturing costs are reduced, and sample processing efficiency is improved.
Smart Images
Figure CN223176089U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of in vitro diagnostic instrument design, and particularly relates to a sample module and a nucleic acid extraction and amplification analyzer capable of lateral feeding. Background Art
[0002] Nucleic acid extraction and PCR amplification (i.e., Polymerase Chain Reaction, a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the organism. The biggest feature of PCR is that it can greatly increase trace amounts of DNA) are important experimental means in molecular biology research. At present, nucleic acid extraction and PCR amplification are mostly carried out separately. Due to excessive manual participation, the experimental process is complicated. At the same time, nucleic acid samples are easily contaminated during the transfer process outside the instrument. Therefore, it is necessary to reasonably and effectively integrate the nucleic acid extraction, PCR amplification and detection processes to avoid excessive manual participation and the transfer of nucleic acid samples outside the instrument. In existing fully automatic nucleic acid extraction and amplification analyzers, the integration degree of storage modules such as sample tubes, Tip head assemblies and reagent cartridges is not high, resulting in the need for the pipette pump assembly configured in the instrument to move in multiple directions between the storage modules to achieve the purpose of inserting and removing Tip heads and aspirating and discharging liquids, which makes the structure and control of the analyzer relatively complex. Summary of the Utility Model
[0003] Therefore, the technical problem to be solved by the utility model is to provide a sample module and a nucleic acid extraction and amplification analyzer capable of lateral feeding, so as to solve the technical problem that in the existing fully automatic nucleic acid extraction and amplification analyzer in the prior art, the integration degree of storage modules such as sample tubes, Tip head assemblies and reagent cartridges is not high, resulting in the need for the pipette pump assembly configured in the instrument to move in multiple directions between the storage modules to achieve the purpose of inserting and removing Tip heads and aspirating and discharging liquids, which makes the structure and control of the analyzer relatively complex.
[0004] To solve the above problems, the utility model provides a sample module capable of lateral feeding, including a sample module bracket, the sample module bracket can be driven to reciprocally slide along a first direction, and a sample tube mounting bracket for accommodating sample tubes and a reagent cartridge mounting plate for accommodating Tip head assemblies and reagent cartridges are sequentially arranged on the sample module bracket along the first direction, wherein the sample tubes, Tip head assemblies and reagent cartridges are sequentially arranged along the first direction.
[0005] In some embodiments, the Tip head assembly includes a tip holder on which a first Tip head and a second Tip head are disposed. The capacity of the first Tip head is greater than that of the second Tip head. A cap placement limiting ring for placing the cap of the sample tube is further formed on the tip holder.
[0006] In some embodiments, the cap placement limiting ring is coaxially arranged with the first Tip head, and the first Tip head is disposed adjacent to the sample tube.
[0007] In some embodiments, the reagent cartridge includes a cartridge body on which a plurality of reagent tubes and PCR tubes are formed. The plurality of PCR tubes are formed on the cartridge body. Each reagent tube and each PCR tube are arranged at intervals in a line along the first direction and are located at one end of the reagent cartridge away from the sample tube.
[0008] In some embodiments, a paraffin ring is pre - placed in each PCR tube, and a paraffin ring heating assembly is provided in the installation groove of the reagent cartridge mounting plate corresponding to the reagent cartridge for heating and melting the paraffin ring.
[0009] In some embodiments, a reagent cartridge pressing plate is provided on the reagent cartridge mounting plate. One side of the reagent cartridge pressing plate is slidably connected to the reagent cartridge mounting plate through a reagent cartridge pressing plate sliding groove to limit the reagent cartridge in the installation groove of the reagent cartridge.
[0010] In some embodiments, a magnet adsorption assembly capable of being controlled to lift and lower is further provided on the sample module bracket for adsorbing the magnetic beads placed in the reagent tube.
[0011] In some embodiments, the sample tube, the Tip head assembly, and the reagent cartridge arranged in a line along the first direction form a sample unit. There are a plurality of such sample units in the sample module, and each sample unit is arranged side by side along a second direction perpendicular to the first direction.
[0012] The present utility model further provides a nucleic acid extraction and amplification analyzer, which includes an overall machine frame. A sample module, a jaw assembly, a pipette pump assembly, and an amplification and detection module are provided on the overall machine frame. Among them, the sample module is the above - mentioned sample module capable of lateral feeding. The jaw assembly is used to open and close the cap of the sample tube. The pipette pump assembly is used to pick up the Tip head in the Tip head assembly and transfer the sample liquid in the sample tube and the reagent in the reagent cartridge between different positions. The amplification and detection module is used to amplify and detect the reaction system in the PCR tube of the reagent cartridge.
[0013] In some embodiments, the sample module, the jaw assembly, and the pipette pump assembly are arranged at intervals along the first direction.
[0014] A sample module and a nucleic acid extraction and amplification analyzer capable of lateral feeding provided by the present utility model integrally arrange a sample tube, a Tip head assembly, and a reagent cartridge on the same sample module bracket at the same time, and arrange the three in a line along the first direction, so that the corresponding pipette pump mechanism can have only the freedom of movement in the lifting direction (i.e., the height direction), and can realize inserting and extracting the Tip head, sucking and discharging the sample liquid in the sample tube, and pipetting between multiple reagent positions of the reagent cartridge. While simplifying the structure, it can also simplify the control logic and reduce the design and manufacturing costs. Description of the Drawings
[0015] Figure 1 is a schematic structural diagram of the sample module capable of lateral feeding in the embodiment of the present utility model
[0016] Figure 2 is Figure 1 a schematic diagram of the reagent cartridge in
[0017] Figure 3 is a schematic diagram of the inside of the reagent cartridge;
[0018] Figure 4 is a schematic structural diagram of the Tip head assembly
[0019] Figure 5 is a schematic diagram after the sample tube is opened;
[0020] Figure 6 is a schematic diagram of the state before the paraffin ring melts;
[0021] Figure 7 is a schematic diagram of the state after the paraffin ring melts and covers the reaction system;
[0022] Figure 8 is a three-dimensional structural diagram (omitting the outer shell) of the sample module of the nucleic acid extraction and amplification analyzer in the embodiment of the present utility model in the pushed-out state.
[0023] The reference numerals are shown as:
[0024] 1. Whole machine frame; 2. Sample module; 201. Sample tube mounting bracket; 202. Sample tube; 203. Tip head assembly; 204. Magnetic adsorption assembly; 206. Reagent cartridge; 207. Reagent cartridge mounting plate; 208. Reagent cartridge pressing plate; 209. Sample module bracket; 210. Reagent cartridge pressing plate chute; 2031. First Tip head; 2032. Second Tip head; 2033. Tube cap placement limiting ring; 2061. Cartridge body; 2062. PCR tube; 2063. Paraffin ring; 2064. Reaction system; 3. Jaw assembly; 4. Pipette pump assembly; 5. Amplification detection module. Detailed implementation manners
[0025] Refer to in combination Figures 1 to 7 As shown, according to the embodiments of the present invention, specifically refer to Figure 1 As shown, a sample module capable of lateral feeding is provided, including a sample module bracket 209, and the sample module bracket 209 can be driven to reciprocally slide along a first direction (refer to Figure 1 the orientation shown, the first direction is also the left - right direction), and a sample tube mounting bracket 201 for accommodating the sample tube 202, and a reagent cartridge mounting plate 207 for accommodating the Tip head assembly 203 and the reagent cartridge 206 are sequentially arranged on the sample module bracket 209 along the first direction. Among them, the sample tube 202, the Tip head assembly 203, and the reagent cartridge 206 are sequentially arranged along the first direction.
[0026] In this technical solution, the sample tube 202, the Tip head assembly 203, and the reagent cartridge 206 are simultaneously integrated and arranged on the same sample module bracket 209, and the three are arranged in a line along the first direction, so that the corresponding pipette pump mechanism can have only the freedom of movement in the lifting direction (i.e., the height direction), and can realize picking up the Tip head, sucking and discharging the sample liquid in the sample tube 202, and pipetting between multiple reagent positions of the reagent cartridge 206. While simplifying the structure, it can also simplify the control logic and reduce the design and manufacturing costs.
[0027] In some embodiments, the Tip head assembly 203 includes a tip head holder, on which a first Tip head 2031 and a second Tip head 2032 are disposed. The capacity of the first Tip head 2031 is greater than that of the second Tip head 2032. For example, the first Tip head can transfer the sample liquid, and the second Tip can be used for accurately aspirating the reagent. A tube cap placement limit ring 2033 for placing the tube cap of the sample tube 202 is further formed on the tip head holder. In this technical solution, integrating the tube cap placement limit ring 2033 on the tip head holder can position and place the opened tube cap on the adjacent sample tube, eliminating the need to separately design a positioning space for placing the tube cap and simplifying the structural design. In a preferred embodiment, the tube cap placement limit ring 2033 is coaxially arranged with the first Tip head 2031, further streamlining the structural design of the tip head holder, making the structure more compact. And according to the nucleic acid extraction process design, placing the tube cap at this position will not prevent the first Tip head 2031 from being discarded at the corresponding position. Preferably, the first Tip head 2031 is arranged adjacent to the sample tube 202, thus greatly improving the efficiency of opening and closing the tube cap.
[0028] In some embodiments, the reagent cartridge 206 includes a cartridge body 2061, on which a plurality of reagent tubes (not labeled in the figure) and PCR tubes 2062 are formed. Each reagent tube is preferably integrally formed with the cartridge body 2061. A plurality of the PCR tubes 2062 are formed on the cartridge body 2061. Each of the reagent tubes and each of the PCR tubes 2062 are arranged at intervals in a line along the first direction and are located at one end of the reagent cartridge 206 away from the sample tube 202. In a specific embodiment, a total of four PCR tubes 2062 are formed on a cartridge body 2061. In this way, four PCR tubes can objectively be assembled in the corresponding card slots of the cartridge body 2061 by using a four-tube PCR tube.
[0029] In this technical solution, the number of the foregoing reagent tubes is related to the specific reagent types required in the specific nucleic acid extraction process and is not particularly limited herein. For example, it includes eluent, cleaning agent, magnetic beads, etc., which will not be elaborated herein. The setting of multiple PCR tubes 2062 enables multiple reaction systems 2064 to be formed at one time in a reagent cartridge 206, which is beneficial for simultaneously detecting multiple items on the same sample liquid and meeting the requirements of multiple detection items.
[0030] In some embodiments, specifically refer to Figure 6As shown, a paraffin ring 2063 is preset in each PCR tube 2062. When not heated, it is in a solid state. At this time, the central through-hole of the preset paraffin ring 2063 is used for the smooth addition of the reaction system 2064 into the tube. The reagent cartridge mounting plate 207 is provided with a paraffin ring heating assembly (not shown in the figure) in the mounting groove (not labeled in the figure) corresponding to the reagent cartridge 206, which is used to heat and melt the paraffin ring 2063. At this time, refer to Figure 7 As shown, the melted paraffin ring 2063 is in a liquid state and is sealed above the liquid level of the reaction system 2064 to prevent the volatilization of the liquid in the system; and / or, a reagent cartridge pressing plate 208 is provided on the reagent cartridge mounting plate 207. One side of the reagent cartridge pressing plate 208 is slidably connected to the reagent cartridge mounting plate 207 through a reagent cartridge pressing plate chute 210 to limit the reagent cartridge 206 in the mounting groove of the reagent cartridge 206, as Figure 1 As shown, the reagent cartridge 206 at this time is limited in the corresponding mounting groove; and / or; a magnet adsorption assembly 204 capable of being controlled to lift and lower is further provided on the sample module bracket 209, which is used to adsorb the magnetic beads placed in the reagent tube. The foregoing magnet adsorption assembly 204 can adopt the relevant structures in the prior art.
[0031] In some embodiments, the sample tubes 202, Tip head assemblies 203, and reagent cartridges 206 arranged in a line along the first direction are one sample unit. There are multiple such sample units in the sample module, and each sample unit is arranged side by side in a second direction perpendicular to the first direction, so as to meet the purpose of high-throughput detection.
[0032] According to an embodiment of the present invention, refer to Figure 8 As shown, a nucleic acid extraction and amplification analyzer is further provided, which includes a whole machine frame 1. A sample module 2, a jaw assembly 3, a liquid transfer pump assembly 4, and an amplification and detection module 5 are provided on the whole machine frame 1. Among them, the sample module is the above-mentioned sample module capable of transverse feeding. The jaw assembly 3 is used to open and close the lid of the sample tube 202. The liquid transfer pump assembly 4 is used to pick up the Tip heads in the Tip head assembly 203 and transfer the sample liquid in the sample tube 202 and the reagents in the reagent cartridge 206 between different positions. The amplification and detection module 5 is used to amplify and detect the reaction system 2064 in the PCR tube 2062 of the reagent cartridge 206. The sample module 2, the jaw assembly 3, and the liquid transfer pump assembly 4 are arranged at intervals along the first direction. The foregoing jaw assembly 3, liquid transfer pump assembly 4, and amplification and detection module 5 can all adopt conventional functional components in the art to achieve the corresponding design. The present invention does not intend to protect these functional components.
[0033] It is easily understandable for those skilled in the art that, on the premise of no conflict, the above-mentioned advantageous ways can be freely combined and superimposed.
[0034] The above are only the preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model. The above is only the preferred implementation manner of the present utility model. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the technical principle of the present utility model, several improvements and variations can still be made, and these improvements and variations should also be regarded as within the protection scope of the present utility model.
Claims
1. A sample module capable of lateral feeding, characterized in that, The invention comprises a sample module bracket (209), wherein the sample module bracket (209) can be driven to slide back and forth along a first direction, and a sample tube mounting bracket (201) for accommodating a sample tube (202), and a reagent cartridge mounting plate (207) for accommodating a Tip head assembly (203) and a reagent cartridge (206) are sequentially arranged on the sample module bracket (209) along the first direction, wherein the sample tube (202), the Tip head assembly (203) and the reagent cartridge (206) are sequentially arranged along the first direction.
2. The sample module according to claim 1, characterized in that, The tip head assembly (203) includes a gun tip bracket, on which a first tip head (2031) and a second tip head (2032) are placed. The capacity of the first tip head (2031) is greater than the capacity of the second tip head (2032). The gun tip bracket is also formed with a tube cover placement limiting ring (2033) for placing the tube cover of the sample tube (202).
3. The sample module according to claim 2, characterized in that, The tube cover placement limiting ring (2033) is coaxially arranged with the first Tip head (2031), and the first Tip head (2031) is arranged adjacent to the sample tube (202).
4. The sample module according to claim 1, characterized in that The reagent cartridge (206) includes a cartridge body (2061), on which a plurality of reagent tubes and PCR tubes (2062) are formed. The plurality of PCR tubes (2062) are formed on the cartridge body (2061), and the reagent tubes and the PCR tubes (2062) are arranged in a line along the first direction and are located at an end of the reagent cartridge (206) away from the sample tube (202).
5. The sample module according to claim 4, wherein A paraffin ring (2063) is pre-installed in each PCR tube (2062), and a paraffin ring heating component is provided in the mounting groove of the reagent cartridge (206) corresponding to the reagent cartridge (206) on the reagent cartridge mounting plate (207) for heating and melting the paraffin ring (2063).
6. The sample module according to claim 4, characterized in that, A reagent cartridge pressing plate (208) is provided on the reagent cartridge mounting plate (207), and one side of the reagent cartridge pressing plate (208) is slidably connected to the reagent cartridge mounting plate (207) via a reagent cartridge pressing plate sliding groove (210) to limit the reagent cartridge (206) to be located within the mounting groove of the reagent cartridge (206).
7. The sample module according to claim 4, characterized in that, The sample module support (209) is also provided with a magnetic adsorption component (204) that can be controlled to rise and fall, and is used to adsorb the magnetic beads placed in the reagent tube.
8. The sample module according to claim 1, characterized in that, The sample tube (202), the tip head assembly (203) and the reagent cartridge (206) arranged in a line along the first direction constitute a sample unit. The sample module contains a plurality of the sample units, and the sample units are arranged side by side along a second direction perpendicular to the first direction.
9. A nucleic acid extraction and amplification analyzer, characterized in that, It includes an overall machine frame (1), on which a sample module (2), a jaw assembly (3), a pipette pump assembly (4) and an amplification and detection module (5) are provided. Among them, the sample module is the sample module capable of lateral feeding described in any one of claims 1 to 8. The jaw assembly (3) is used to open and close the lid of the sample tube (202). The pipette pump assembly (4) is used to pick up the tips in the Tip head assembly (203) and transfer the sample liquid in the sample tube (202) and the reagents in the reagent cartridge (206) between different positions. The amplification and detection module (5) is used to amplify and detect the reaction system (2064) in the PCR tube (2062) of the reagent cartridge (206).
10. The nucleic acid extraction and amplification analyzer according to claim 9, wherein The sample module (2), the jaw assembly (3) and the pipette pump assembly (4) are arranged at intervals along the first direction.