Sorting consumable pipe group and sample processing equipment

Abstract

The utility model provides a sorting consumable pipe group and sample processing equipment. The sorting consumable pipe group comprises a centrifugal container with a conical centrifugal space, a first main pipeline used for being mounted to a driving pump, and a second main pipeline communicated with an air port, the rotating shaft is located on the side, away from the conical tip of the conical centrifugal space, of the centrifugal container; the first end of the first main pipeline is communicated with the sample container and the first collection container, and the second end of the first main pipeline is communicated with the conical tip of the conical centrifugal space; one end of the second main pipeline away from the air port is communicated with the conical bottom end of the conical centrifugal space. According to the utility model, the non-target cells and the combined cells are separated, meanwhile, the damage to the target cells is avoided, the operation is simple and convenient, and the high recovery rate and high purity of sorting can be ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedicine, and particularly relates to a sorting consumable tube set and a sample processing device. Background Art

[0002] At present, in the field of cell pharmaceuticals, it is often necessary to sort out a certain specific target cell from a cell solution with composite components, process it and form a drug. In the prior art, the principle of antigen-antibody binding can be utilized to enable the target cell to specifically bind to magnetic beads, so as to capture the magnetic beads by magnetic force, and thus complete the screening of the target cell. However, the above magnetic sorting technology has the following defects: If nano magnetic beads are used for magnetic sorting, a sorting column must be used in cooperation. The sorting column is a porous medium structure. The target cells are adsorbed on the sorting column, and the non-target cells flow out of the sorting column. During the above process of magnetic sorting through the sorting column, the target cells need to be simultaneously subjected to the fluid shear force inside the porous medium and the magnetic force of the magnetic field, so that the cells are easily damaged or even broken. In addition, after the target cells are adsorbed on the sorting column, when the target cells in the sorting column need to be eluted, at this time, due to the existence of the porous medium, the target cells are easily hidden in the gaps, which will also result in a low yield of the target cells, generally only 60%-80%. If it is a patient sample with fragile cells, the yield will be even lower. If micron magnetic beads are used for magnetic sorting, when making drugs from cells, there are strict requirements for the residue of micron magnetic beads. To meet the requirements, it is necessary to further add a process for removing magnetic beads. The above process will also have problems such as low purity and inconvenient operation. Summary of the Utility Model

[0003] Aiming at the technical problems of low yield and low purity in magnetic sorting of target cells in the prior art, the utility model provides a sorting consumable tube set and a sample processing device.

[0004] In view of the above technical problems, an embodiment of the utility model provides a sorting consumable tube set, which includes a centrifugal container with a conical centrifugal space, a first main pipeline for installing to a driving pump, and a second main pipeline communicating with an air port; the centrifugal container rotates around a rotation axis, and the rotation axis is located on one side of the centrifugal container away from the conical tip of the conical centrifugal space;

[0005] The first end of the first main pipeline communicates with a sample container and a first collection container, and the second end of the first main pipeline communicates with the conical tip of the conical centrifugal space; the end of the second main pipeline away from the air port communicates with the conical bottom end of the conical centrifugal space.

[0006] Optionally, the centrifugal container includes a container body, a first delivery pipe and a second delivery pipe; the conical centrifugal space is arranged on the container body;

[0007] The first delivery pipe is inserted into the conical tip of the conical centrifugal space, and the second delivery pipe is inserted into the conical bottom end of the conical centrifugal space. The second end of the first main pipeline communicates with the conical tip through the first delivery pipe; the second end of the second main pipeline communicates with the conical bottom end through the second delivery pipe.

[0008] Optionally, the central axis of the conical centrifugal space is perpendicular to and lies in the same plane as the rotation axis.

[0009] Optionally, the sorting consumable pipe group further includes an air filter installed at the air port.

[0010] Optionally, the first main pipeline is also installed on a first control valve, and the first control valve is located between the centrifugal container and the driving pump; the sorting consumable pipe group further includes a first cleaning pipeline connected between the first cleaning container and the first main pipeline, and the first connection point between the first cleaning pipeline and the first main pipeline is located between the first control valve and the driving pump.

[0011] Optionally, the sorting consumable pipe group further includes a second cleaning pipeline connected between the second cleaning container and the first main pipeline, and the second connection point between the second cleaning pipeline and the first main pipeline is located on the side of the driving pump away from the centrifugal container.

[0012] Optionally, the sorting consumable pipe group further includes a second collection pipeline connected between the second collection container and the first main pipeline, and the third connection point between the second collection pipeline and the first main pipeline is located on the side of the driving pump away from the centrifugal container.

[0013] Optionally, the sorting consumable pipe group further includes a waste liquid pipeline connected between the waste liquid container and the second main pipeline, and the fourth connection point between the waste liquid pipeline and the second main pipeline is located between the air port and the centrifugal container.

[0014] The present utility model also provides a sample processing device, including a driving pump and the sorting consumable pipe group as described above.

[0015] Optionally, the sample processing device further includes a second control valve provided between the air port and the centrifugal container, and a pressure sensor provided on the first main pipeline and located between the driving pump and the centrifugal container, where the pressure sensor is used to detect the pressure in the first main pipeline communicating with the conical centrifugal space when the second control valve controls the second main pipeline to be closed; and / or

[0016] The sample processing device further includes a bubble sensor disposed between the sample container and the centrifugation container, and the bubble sensor is used to detect whether the sample liquid in the sample container has drained out.

[0017] In the present utility model, the sorting consumable tube set includes a centrifugation container having a conical centrifugation space, a first main pipeline for installation to a driving pump, and a second main pipeline communicating with an air port; the centrifugation container rotates around a rotation axis, and the rotation axis is located on a side of the centrifugation container away from the conical tip of the conical centrifugation space; a first end of the first main pipeline communicates with the sample container and a first collection container, and a second end of the first main pipeline communicates with the conical tip of the conical centrifugation space; an end of the second main pipeline away from the air port communicates with the conical bottom end of the conical centrifugation space.

[0018] In the present utility model, when it is necessary to sort a sample liquid (the sample liquid includes non-target cells and combined cells obtained after combining target cells with microbubbles), first, the driving pump is used to drive the sample liquid in the sample container to be injected into the conical centrifugation space of the centrifugation container through the first main pipeline. After that, the centrifugation container is controlled to rotate around the rotation axis (the rotation axis is located on a side of the centrifugation container away from the conical tip of the conical centrifugation space), and then the sample liquid can be swung to a side close to the conical tip of the conical centrifugation space. Moreover, the combined cells and non-target cells will be layered. Specifically, since there are microbubbles in the combined cells in the sample liquid, the combined cells will be located on a side of the sample liquid close to the rotation axis, that is, at the gas-liquid interface position of the sample liquid in the conical centrifugation space. The non-target cells are relatively heavy because they do not bind microbubbles. Therefore, the non-target cells will be located at the conical tip of the conical centrifugation space. At this time, the non-target cells located at the conical tip can be exported to the first collection container through the first main pipeline communicating with the conical tip, and at the same time, the combined cells located at the gas-liquid interface position close to the conical bottom end are retained in the conical centrifugation space, thereby realizing the separation of non-target cells and combined cells. In the present utility model, without using a sorting column, the above sorting consumable tube set can be used to separate non-target cells from combined cells (that is, target cells), and damage to the target cells is avoided during the separation process, and there is no need to add a process for removing magnetic beads, etc. The operation is simple, and high recovery rate and high purity of sorting can be ensured. Description of the Drawings

[0019] The present utility model will be further described below with reference to the drawings and embodiments.

[0020] Figure 1 is a schematic structural diagram of a sorting consumable tube set provided by an embodiment of the present utility model;

[0021] Figure 2 is a schematic structural diagram of a centrifugation container of a sorting consumable tube set provided by an embodiment of the present utility model.

[0022] The reference numerals in the description are as follows:

[0023] 1. Centrifugal container; 110. Conical centrifugal space; 111. Conical tip; 112. Conical bottom end; 120. Container body; 130. First delivery pipe; 140. Second delivery pipe; 2. First main pipeline; 21. First control valve; 3. Second main pipeline; 4. Air port; 41. Second control valve; 5. Driving pump; 6. Rotating shaft; 7. Sample container; 8. Sample pipeline; 81. First switching valve; 9. First collection container; 10. First collection pipeline; 101. Second switching valve; 11. First cleaning container; 12. First cleaning pipeline; 121. Third switching valve; 13. Second cleaning container; 14. Second cleaning pipeline; 141. Fourth switching valve; 15. Second collection container; 16. Second collection pipeline; 161. Fifth switching valve; 17. Waste liquid container; 18. Waste liquid pipeline; 181. Sixth switching valve; 19. Pressure sensor; 20. Bubble sensor. Detailed implementation manners

[0024] In order to make the technical problems, technical solutions and beneficial effects solved by the present utility model clearer and more understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0025] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "longitudinal", "radial", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In the description of the present utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0026] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0027] As Figures 1 to 2As shown in the figure, an embodiment of the present utility model provides a sorting consumable tube set, including a centrifuge container 1 with a conical centrifugal space 110, a first main pipeline 2 for installation to a drive pump 5, and a second main pipeline 3 communicating with an air port 4; the centrifuge container 1 rotates around a rotation axis 6, and the rotation axis 6 is located on a side of the centrifuge container 1 away from a conical tip 111 of the conical centrifugal space 110; a first end of the first main pipeline 2 communicates with a sample container 7 and a first collection container 9, and a second end of the first main pipeline 2 communicates with the conical tip 111 of the conical centrifugal space 110; an end of the second main pipeline 3 away from the air port 4 communicates with a conical bottom end 112 of the conical centrifugal space 110.

[0028] In the present utility model, the sample liquid to be sorted includes non-target cells and combined cells obtained by combining target cells with microbubbles (microbubbles refer to particles containing gas or other low-density components. For example, microbubbles can be micron-sized phospholipid bubbles, micron-sized glass vacuoles, micron-sized polymer vacuoles, etc.), and the density of the combined cells containing the bound microbubbles is less than that of water. Since the centrifuge container 1 can rotate around the rotation axis 6, and the conical tip 111 of the conical centrifugal space 110 is located on a side of the centrifuge container 1 away from the rotation axis 6, when the centrifuge container 1 rotates at a high speed around the rotation axis 6, the sample liquid can be flung to a side close to the conical tip 111 of the conical centrifugal space 110. Moreover, there are microbubbles in the combined cells in the sample liquid and the density is less than that of water. Therefore, the combined cells will be located on a side of the sample liquid close to the rotation axis 6, that is, at the gas-liquid interface position of the sample liquid in the conical centrifugal space 110. Non-target cells are relatively heavy (density greater than water) because they do not bind to microbubbles. Therefore, the non-target cells will be located at the conical tip 111 of the conical centrifugal space 110, and thus the separation of the combined cells and non-target cells is achieved under density gradient centrifugation.

[0029] In the present utility model, the rotating shaft 6 only needs to be arranged on one side of the centrifugal container 1 away from the conical tip 111 of the conical centrifugal space 110, and the above-mentioned stratification of combined cells and non-target cells can be achieved. Therefore, the relative position relationship between the rotating shaft 6 and the conical centrifugal space 110 can be set according to specific requirements. In one embodiment, the central axis of the conical centrifugal space 110 is perpendicular to the rotating shaft 6 and lies in the same plane. In this way, when the centrifugal container 1 rotates around the rotating shaft 6, the sample liquid in the conical centrifugal space 110 can be symmetrically distributed with the central axis as the axis of symmetry, and further, the distribution of the sample liquid in the conical centrifugal space 110 is more uniform, facilitating the progress of the cell processing process. It can be understood that the conical centrifugal space 110 is not limited to a conical shape, and can also be set as a splicing shape of a cone and other shapes, or an approximate conical shape. For example, the conical tip 111 can be set as a conical shape, and the conical tail end can be set as other shapes, such as a cylindrical shape; the entire conical centrifugal space 110 can also be set as a frustum of a cone, and the small-diameter end of the frustum of the cone is the conical tip 111; the conical centrifugal space 110 can also be set as a pyramid, etc., which is not limited herein; as long as the above-mentioned stratification effect of combined cells and non-target cells can be achieved.

[0030] It can be understood that in this embodiment, first, when the centrifugal container 1 does not rotate around the rotating shaft 6, the driving pump 5 is used to drive the sample liquid in the sample container 7 to be injected into the conical centrifugal space 110 of the centrifugal container 1 through the first main pipeline 2. After that, the centrifugal container 1 is controlled to rotate around the rotating shaft 6, and the sample liquid can be swung to one side close to the conical tip 111 of the conical centrifugal space 110. Moreover, the combined cells and non-target cells will be stratified. Specifically, since there are microbubbles in the combined cells in the sample liquid, the combined cells will be located on one side of the sample liquid close to the rotating shaft 6, that is, at the gas-liquid interface position of the sample liquid in the conical centrifugal space 110. The non-target cells are relatively heavy because they are not combined with microbubbles. Therefore, the non-target cells will be located at the conical tip 111 of the conical centrifugal space 110. At this time, the non-target cells located at the conical tip 111 can be led out to the first collection container 9 through the first main pipeline 2 communicating with the conical tip 111, and the combined cells located at the gas-liquid interface position close to the conical bottom end 112 are retained in the conical centrifugal space 110, thereby realizing the separation of non-target cells and combined cells.

[0031] Understandably, the sample container 7 can be connected to the first end of the first main pipeline 2 through the sample pipeline 8, and a first switching valve 81 for controlling the on-off of the sample pipeline 8 is provided on the sample pipeline 8. Further, the first collection container 9 can be connected to the first end of the first main pipeline 2 through the first collection pipeline 10, and a second switching valve 101 for controlling the on-off of the first collection pipeline 10 is provided on the first collection pipeline 10. Thus, in this embodiment, when the first switching valve 81 is opened and the second switching valve 101 is closed, the driving pump 5 can be controlled to drive the sample liquid in the sample container 7 to be sequentially injected into the conical centrifugal space 110 through the first main pipeline 2 and the first delivery pipe 130; and when both the first switching valve 81 and the second switching valve 101 are closed, the centrifugal container 1 can be controlled to rotate around the rotation axis 6 so that the combined cells in the sample liquid are located at the gas-liquid interface close to the rotation axis 6 in the sample liquid, and the non-target cells are located at the conical tip 111; the rotation axis 6 is located on the side of the centrifugal container 1 away from the conical tip 111; furthermore, after the first switching valve 81 is closed and the second switching valve 101 is opened, the driving pump 5 can be controlled to drive the sample liquid in the conical centrifugal space 110 to enter the first collection container 9 from the conical tip 111 sequentially through the first delivery pipe 130 and the first main pipeline 2. That is, in this embodiment, negative selection is performed on the sample liquid through the sorting consumable pipe group, that is, the non-target cells are separated from the sample liquid and collected into the first collection container 9, while the combined cells are retained in the conical centrifugal space 110.

[0032] Understandably, after the non-target cells enter the first collection container 9, a certain volume of sample liquid (such as a preset volume set according to the volume of the conical centrifugal space 110 and determined after experimental verification, where the preset volume can be set according to requirements) needs to be retained in the conical centrifugal space 110 to ensure that the combined cells do not flow out of the conical centrifugal space 110. In the present utility model, an optical liquid level sensor can be installed above the centrifugal container 1 to monitor the liquid level in the conical centrifugal space to ensure that a preset volume of sample liquid is retained in the conical centrifugal space 110. It is also possible to control the volume through a high-precision preparation module, and then control that a preset volume of sample liquid is retained in the conical centrifugal space 110.

[0033] In the present utility model, when it is necessary to sort a sample liquid (the sample liquid includes non-target cells and combined cells obtained by combining target cells with microbubbles), first, a driving pump 5 drives the sample liquid in the sample container 7 to be injected into the conical centrifugal space 110 of the centrifugal container 1 through the first main pipeline 2. Then, the centrifugal container 1 is controlled to rotate around the rotation axis 6 (the rotation axis 6 is located on one side of the centrifugal container 1 away from the conical tip 111 of the conical centrifugal space 110). In this way, the sample liquid can be swung to one side close to the conical tip 111 of the conical centrifugal space 110, and the combined cells and non-target cells will be layered. Specifically, since there are microbubbles in the combined cells in the sample liquid, the combined cells will be located on the side close to the rotation axis 6 in the sample liquid, that is, at the gas-liquid interface position of the sample liquid in the conical centrifugal space 110. The non-target cells are relatively heavy because they are not combined with microbubbles. Therefore, the non-target cells will be located at the conical tip 111 of the conical centrifugal space 110. At this time, the non-target cells located at the conical tip 111 can be exported to the first collection container 9 through the first main pipeline 2 communicating with the conical tip 111, and the combined cells located at the gas-liquid interface position close to the conical bottom end 112 are retained in the conical centrifugal space 110, thereby realizing the separation of non-target cells and combined cells. In the present utility model, without using a sorting column, the above sorting consumable tube set can be used to separate non-target cells from combined cells (i.e., target cells), and damage to the target cells is avoided during the separation process, and there is no need to add a process for removing magnetic beads, etc. The operation is simple, and high recovery rate and high purity of sorting can be ensured.

[0034] In one embodiment, as Figure 1 and Figure 2 shown, the centrifugal container 1 includes a container body 120, a first delivery pipe 130, and a second delivery pipe 140; the conical centrifugal space 110 is provided on the container body 120; the first delivery pipe 130 is inserted into the conical tip 111 of the conical centrifugal space 110, the second delivery pipe 140 is inserted into the conical bottom end 112 of the conical centrifugal space 110, and the second end of the first main pipeline 2 communicates with the conical tip 111 through the first delivery pipe 130; the second end of the second main pipeline 3 communicates with the conical bottom end 112 through the second delivery pipe 140. It can be understood that the shape of the container body 120 can be set according to requirements. For example, the container body 120 can also be set as a cone. And the central axis of the conical container body 120 coincides with the central axis of the conical centrifugal space 110, and the tip of the conical container body 120 is in the same direction as the conical tip 111 of the conical centrifugal space 110. However, the shape of the container body 120 can also be set as other shapes according to requirements, such as cylindrical, square, etc., and no limitation is made here.

[0035] Furthermore, asFigure 2 As shown, one end of the first delivery pipe 130 away from the first main pipeline 2 and one end of the first delivery pipe 130 away from the second delivery pipe 140 are both inserted into the conical centrifugal space 110 from the conical bottom end 112; that is, in this embodiment, both the first delivery pipe 130 and the second delivery pipe 140 are inserted from the conical bottom end 112, but the first delivery pipe 130 will directly insert the pipe orifice into the conical tip 111 from the conical bottom end 112, while the second delivery pipe 140 can directly insert the pipe orifice into the conical bottom end 112.

[0036] Further, one end of the first delivery pipe 130 away from the first main pipeline 2 is inserted into the conical centrifugal space 110 from the conical tip 111, and one end of the first delivery pipe 130 away from the second delivery pipe 140 is inserted into the conical centrifugal space 110 from the conical bottom end 112. That is, in this embodiment, the first delivery pipe 130 is directly inserted into the conical centrifugal space 110 from the conical tip 111, and the inserted pipe orifice is also located at the conical tip 111; the second delivery pipe 140 is inserted from the conical bottom end 112, and the inserted pipe orifice is located at the conical bottom end 112.

[0037] It can be understood that since the total density of the combined cells after the target cells are combined with the microbubbles is less than that of water after the sample liquid enters the conical centrifugal space 110, while the density of the non-target cells is greater than that of water, therefore, under the treatment of density gradient centrifugation, the target cells will float on the liquid surface of the sample liquid, and the non-target cells will sink to the bottom of the sample liquid, and the two are separated; at this time, in order to prevent the combined cells located on the liquid surface from overflowing from the pipe orifice where the second delivery pipe 140 is inserted into the conical centrifugal space 110, it is necessary to control the liquid level height of the sample liquid entering the conical centrifugal space 110 to be less than the pipe orifice of the second delivery pipe 140. Therefore, in some embodiments, the inserted pipe orifice of the second delivery pipe 140 is located above the conical centrifugal space 110, so that the capacity of the sample liquid that can be input into the conical centrifugal space 110 can be increased, thereby increasing the capacity of the sample liquid that can be sorted at one time, and further improving the cell sorting efficiency.

[0038] In one embodiment, the sorting consumable pipe group further includes an air filter installed at the air port 4. That is, in this embodiment, an air filter is installed at the air port 4 to ensure that the gas entering the second main pipeline 3 from the external environment is sterile, and further ensure the sterile environment in the conical centrifugal space 110. Among them, a second control valve 41 is provided between the air port 4 and the centrifugal container 1. The second control valve 41 can be provided on the second main pipeline 3, or a ventilation pipeline connecting the second main pipeline 3 can be provided, and then the second control valve 41 can be provided on the ventilation pipeline. There is no limitation here.

[0039] In one embodiment, as Figure 1As shown, the first main pipeline 2 is also installed on the first control valve 21, and the first control valve 21 is located between the centrifugal container 1 and the driving pump 5; the sorting consumable pipe group further includes a first cleaning pipeline 12 communicating between the first cleaning container 11 and the first main pipeline 2, and the first connection point between the first cleaning pipeline 12 and the first main pipeline 2 is located between the first control valve 21 and the driving pump 5. Understandably, a third switch valve 121 for controlling the on / off of the first cleaning pipeline 12 is provided on the first cleaning pipeline 12. In this embodiment, after closing the first control valve 21 (the third switch valve 121 and the second switch valve 101 are opened, and the rest of the switch valves are closed), at this time, the cleaning liquid in the first cleaning container 11 is driven by the driving pump 5 to flow through the first cleaning pipeline 12 and the first main pipeline 2 (and the first collection pipeline 10) into the first collection container 9, and then a part of the first main pipeline 2 close to the first collection container 9 (and the first collection pipeline 10) can be cleaned, and the non-target cells remaining in the pipeline are flushed into the first collection container 9.

[0040] In one embodiment, as Figure 1 shown, the sorting consumable pipe group further includes a second cleaning pipeline 14 communicating between the second cleaning container 13 and the first main pipeline 2, and the second connection point between the second cleaning pipeline 14 and the first main pipeline 2 is located on the side of the driving pump 5 away from the centrifugal container 1. Understandably, a fourth switch valve 141 for controlling the on / off of the second cleaning pipeline 14 is provided on the second cleaning pipeline 14. In this embodiment, after opening the first control valve 21 and the second control valve 41 (the fourth switch valve 141 is opened, and the rest of the switch valves are closed), the cleaning liquid in the second cleaning container 13 is driven by the driving pump 5 to flow through the second cleaning pipeline 14, the first main pipeline 2 and the first delivery pipe 130 into the conical centrifugal space 110, and air can enter the conical centrifugal space 110 from the air port 4 position through the second main pipeline 3 to balance the air pressure. In the above process, cleaning liquid can be injected into the conical centrifugal space 110 to clean the first main pipeline 2 and the first delivery pipe 130, and the sample liquid remaining in the pipeline is flushed into the conical centrifugal space 110. Understandably, in the above process, the cleaning liquid cannot exceed the pipe orifice where the second delivery pipe 140 is inserted into the conical centrifugal space 110 to avoid the combined cells from overflowing from this pipe orifice.

[0041] Understandably, the above process of injecting cleaning liquid through the first cleaning container 11 and the second cleaning container 13 to clean the pipeline can be repeated multiple times to enhance the cleaning effect.

[0042] In a further embodiment, after the above cleaning operation is completed, the rotation of the centrifugation container 1 around the rotation axis 6 can be stopped, so that the sample liquid containing the combined cells is stationary in the conical centrifugation space 110. Then, the first control valve 21 can be controlled to open, and the second control valve 41 can be closed (the fourth switching valve 141 is open, and the rest of the switching valves are closed) to disconnect the passage between the conical centrifugation space 110 and the air port 4. The cleaning liquid in the second cleaning container 13 is driven by the driving pump 5 to enter the conical centrifugation space 110 through the first main pipeline 2 and the first delivery pipe 130 to increase the pressure in the conical centrifugation space 110. Then, after the pressure in the conical centrifugation space 110 is increased to the preset annihilation pressure and maintained for a preset duration, it is confirmed that the microbubbles in the combined cells in the sample liquid are all annihilated, and thus the target cells are obtained. In this way, the microbubbles in the combined cells can be annihilated and removed through this embodiment, realizing the separation of the microbubbles and the target cells. Among them, both the preset annihilation pressure and the preset duration can be set according to requirements. The preset annihilation pressure needs to be greater than the atmospheric pressure.

[0043] It can be understood that in the present utility model, a pressure sensor 19 is provided on the first main pipeline 2. The pressure sensor 19 is located between the driving pump 5 and the centrifugation container 1. The pressure sensor 19 is used to detect the pressure in the first main pipeline 2 communicated with the conical centrifugation space 110 when the second control valve 41 controls the second main pipeline 3 to be closed. Specifically, the pressure information in the first main pipeline 2 is monitored in real time by the pressure sensor 19, and the real-time pressure value is determined according to the pressure information. When the real-time pressure value is greater than or equal to the preset annihilation pressure, it is confirmed that the pressure in the conical centrifugation space 110 has increased to the preset annihilation pressure.

[0044] In one embodiment, as Figure 1As shown, the sorting consumable tube group further includes a second collection pipeline 16 connected between the second collection container 15 and the first main pipeline 2. The third connection point between the second collection pipeline 16 and the first main pipeline 2 is located on the side of the drive pump 5 away from the centrifugal container 1. Understandably, a fifth switch valve 161 for controlling the on / off of the second collection pipeline 16 is provided on the second collection pipeline 16. In this embodiment, after confirming that the microbubbles in the combined cells are all annihilated, the first control valve 21 can be controlled to open, and the second control valve 41 can be opened (while controlling the centrifugal container 1 to rotate at a low speed or remain stationary to prevent the sample liquid from spraying out of the nozzle of the second delivery pipe 140), so as to connect the conical centrifugal space 110 with the air port 4 and reduce the pressure in the conical centrifugal space 110 to be consistent with the external atmospheric pressure to release the pressure in the conical centrifugal space 110; then, control the centrifugal container 1 to rotate around the rotation axis 6. At this time, since the microbubbles in the combined cells in the conical centrifugal space 110 have been annihilated, the density of the target cells of the remaining annihilated microbubbles will be greater than that of water. Therefore, the target cells of the annihilated microbubbles will be located at the conical tip 111 under the action of centrifugal force. At this time, control the fifth switch valve 161 to open and the other switch valves to close, and the target cells of the annihilated microbubbles in the conical centrifugal space 110 can be driven by the drive pump 5 to be led out to the second collection container 15 through the first delivery pipe 130 and the first main pipeline 2. Thus, after sorting the non-target cells into the first collection container 9, the target cells after removing the microbubbles are also sorted into the second collection container 15. Understandably, in this embodiment, the sample liquid is subjected to positive selection by the sorting consumable tube group, that is, the non-target cells are separated from the sample liquid and collected into the first collection container 9, and the target cells are collected into the second collection container 15. In the present utility model, the separation of the target cells and the non-target cells can be achieved without setting a sorting column, and cell damage is avoided.

[0045] In one embodiment, as Figure 1As shown, the sorting consumable tube group further includes a waste liquid pipeline 18 connected between the waste liquid container 17 and the second main pipeline 3. The fourth connection point between the waste liquid pipeline 18 and the second main pipeline 3 is located between the air port 4 and the centrifugal container 1. Understandably, a sixth switch valve 181 for controlling the on / off of the waste liquid pipeline 18 is provided on the waste liquid pipeline 18. That is, in this embodiment, before sorting the target cells into the second collection container 15, in order to reduce the volume of the finally obtained preparation, a elutriation operation can also be performed on the sample liquid containing the target cells with the microbubbles that have been annihilated to remove the waste liquid in the sample liquid in the conical centrifugal space. Specifically, control the first control valve 21 to open and the second control valve 41 to close to disconnect the connection between the air port 4 and the second main pipeline 3. At the same time, open the sixth switch valve 181 and the fourth switch valve 141 (the rest of the switch valves are closed) so that the waste liquid pipeline 18 is connected to the conical centrifugal space 110 through the second main pipeline 3, and the second cleaning container 13 is connected to the first main pipeline 2 through the cleaning pipeline; then, control the centrifugal container 1 to rotate around the rotation axis 6. At this time, since the microbubbles in the combined cells in the conical centrifugal space 110 have been annihilated, the density of the remaining target cells with the annihilated microbubbles will be greater than that of water. Therefore, the target cells with the annihilated microbubbles will be located at the conical tip 111 under the action of centrifugal force, while the waste liquid is located on the side close to the conical tail end in the conical centrifugal space 110. At this time, the cleaning liquid in the second cleaning container 13 can be driven by the driving pump 5 to enter the conical centrifugal space 110 through the cleaning pipeline, the first main pipeline 2 and the first delivery pipe 130, so that the liquid in the conical centrifugal space 110 covers the pipe orifice of the second delivery pipe 140 inserted into the conical bottom end 112 under the action of centrifugation. Furthermore, the sample liquid covering the pipe orifice of the second delivery pipe 140 is the waste liquid. Therefore, the waste liquid in the liquid except for the target cells with the annihilated microbubbles can be extracted into the waste liquid container 17 through the second delivery pipe 140 and the second main pipeline 3; while the target cells with the annihilated microbubbles that are always located at the conical tip 111 will be retained in the conical centrifugal space 110.

[0046] The present utility model further provides a sample processing device, including a driving pump 5 and the sorting consumable tube group as described above. Among them, the driving pump 5 can be a peristaltic pump or other pumps that can drive the fluid in the pipeline, and its specific type is not limited herein.

[0047] In the present utility model, when it is necessary to sort a sample liquid (the sample liquid includes non-target cells and combined cells obtained by combining target cells with microbubbles), first, a driving pump 5 drives the sample liquid in a sample container 7 to be injected into a conical centrifugal space 110 of a centrifugal container 1 through a first main pipeline 2. After that, the centrifugal container 1 is controlled to rotate around a rotation axis 6 (the rotation axis 6 is located on a side of the centrifugal container 1 away from a conical tip 111 of the conical centrifugal space 110), so that the sample liquid can be swung to a side close to the conical tip 111 of the conical centrifugal space 110. Moreover, the combined cells and non-target cells will be stratified. Specifically, since there are microbubbles in the combined cells in the sample liquid, the combined cells will be located on a side of the sample liquid close to the rotation axis 6, that is, at the gas-liquid interface position of the sample liquid in the conical centrifugal space 110. The non-target cells are relatively heavy because they are not combined with microbubbles. Therefore, the non-target cells will be located at the conical tip 111 of the conical centrifugal space 110. At this time, the non-target cells located at the conical tip 111 can be led out to a first collection container 9 through the first main pipeline 2 communicating with the conical tip 111, and the combined cells located at the gas-liquid interface position close to a conical bottom end 112 are retained in the conical centrifugal space 110, thereby realizing the separation of the non-target cells and the combined cells. In the present utility model, without using a sorting column, the above sorting consumable tube set can be used to separate non-target cells from combined cells (i.e., target cells), and damage to the target cells is avoided during the separation process, and there is no need to add a process for removing magnetic beads, etc. The operation is simple, and high recovery rate and high purity of sorting can be ensured.

[0048] In one embodiment, as Figure 1 shown, the sample processing device further includes a second control valve 41 disposed between the air port 4 and the centrifugal container 1, and a pressure sensor 19 disposed on the first main pipeline 2 and between the driving pump 5 and the centrifugal container 1. The pressure sensor 19 is used to detect the pressure in the first main pipeline 2 communicating with the conical centrifugal space 110 when the second control valve 41 controls the second main pipeline 3 to be closed. Specifically, the pressure information in the first main pipeline 2 is monitored in real time by the pressure sensor 19, and a real-time pressure value is determined according to the pressure information. When the real-time pressure value is greater than or equal to the preset annihilation pressure, it is confirmed that the pressure in the conical centrifugal space 110 increases to the preset annihilation pressure.

[0049] In one embodiment, as Figure 1As shown, the sample processing device further includes a bubble sensor 20 disposed between the sample container 7 and the centrifugal container 1. The bubble sensor 20 is used to detect whether the sample liquid in the sample container 7 has completely drained. Further, the bubble sensor 20 is located between the drive pump 5 and the sample container 7. Furthermore, when the volume of the sample liquid in the sample container 7 is too large and the centrifugal container 1 cannot process it all at once, if necessary, the above-mentioned sorting consumable tube set can be used for repeated processing. At this time, it is necessary to use the bubble sensor 20 to determine whether all the sample liquid in the sample container 7 has drained, that is, to determine whether all the sample liquid in the sample container 7 has been completely processed.

[0050] The above are only examples of the sorting consumable tube set and the sample processing device of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A sorting consumable tube set, characterized in that, It includes a centrifugal container with a conical centrifugal space, a first main pipeline for installation to a driving pump, and a second main pipeline communicating with an air port; the centrifugal container rotates around a rotation axis, and the rotation axis is located on one side of the centrifugal container away from the conical tip of the conical centrifugal space; The first end of the first main pipeline communicates with a sample container and a first collection container, and the second end of the first main pipeline communicates with the conical tip of the conical centrifugal space; the end of the second main pipeline away from the air port communicates with the conical bottom end of the conical centrifugal space.

2. The sorting consumable tube set according to claim 1, wherein, The centrifugal container includes a container body, a first delivery pipe, and a second delivery pipe; the conical centrifugal space is arranged on the container body; The first delivery pipe is inserted into the conical tip of the conical centrifugal space, the second delivery pipe is inserted into the conical bottom end of the conical centrifugal space, and the second end of the first main pipeline communicates with the conical tip through the first delivery pipe; the second end of the second main pipeline communicates with the conical bottom end through the second delivery pipe.

3. The sorting consumable tube set according to claim 2, wherein The central axis of the conical centrifugal space is perpendicular to the rotation axis and lies in the same plane.

4. The sorting consumable tube set according to claim 1, characterized in that, The sorting consumable pipe group further includes an air filter installed at the air port.

5. The sorting consumable tube set according to claim 1, wherein The first main pipeline is also installed on a first control valve, and the first control valve is located between the centrifugal container and the driving pump; the sorting consumable pipe group further includes a first cleaning pipeline communicating between a first cleaning container and the first main pipeline, and the first connection point between the first cleaning pipeline and the first main pipeline is located between the first control valve and the driving pump.

6. The sorting consumable tube set according to claim 1, wherein, The sorting consumable pipe group further includes a second cleaning pipeline communicating between a second cleaning container and the first main pipeline, and the second connection point between the second cleaning pipeline and the first main pipeline is located on the side of the driving pump away from the centrifugal container.

7. The sorting consumable tube set according to claim 6, wherein The sorting consumable pipe group further includes a second collection pipeline communicating between a second collection container and the first main pipeline, and the third connection point between the second collection pipeline and the first main pipeline is located on the side of the driving pump away from the centrifugal container.

8. The sorting consumable tube set according to claim 6, wherein, The sorting consumable pipe group further includes a waste liquid pipeline communicating between a waste liquid container and the second main pipeline, and the fourth connection point between the waste liquid pipeline and the second main pipeline is located between the air port and the centrifugal container.

9. A sample processing device, characterized in that, It includes a driving pump and the sorting consumable pipe group according to any one of claims 1 to 8.

10. The sample processing device according to claim 9, wherein, The sample processing device further includes a second control valve arranged between the air port and the centrifugal container, and a pressure sensor arranged on the first main pipeline and located between the driving pump and the centrifugal container, and the pressure sensor is used to detect the pressure in the first main pipeline communicating with the conical centrifugal space when the second control valve controls the second main pipeline to be closed; and / or The sample processing device further includes a bubble sensor arranged between the sample container and the centrifugal container, and the bubble sensor is used to detect whether the sample liquid in the sample container has run out.

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