Pressurized culture bottle capable of sampling in fixed layer and selectively aerating

Abstract

The utility model discloses a pressurized culture bottle capable of sampling in a fixed layer and selectively aerating, which comprises a bottle body, and a fixed layer sampling system, an air pressure stabilizing system and an air inlet selecting and refining system are arranged in the bottle body. According to the utility model, pressurization, gas replacement and aeration are realized through the gas inlet selection and gas refining system; pressurizing is completed by keeping air inlet and closing exhaust; closing the gas refining pipe, opening the gas inlet pipe in the bottle, and carrying out gas replacement; the gas refining pipe is opened, the gas inlet pipe in the bottle is closed, at the moment, gas enters the bottom of a culture solution from small gas passages uniformly distributed on the gas refining pipe for aeration, a fixed-layer sampling system, a gas pressure stabilizing system and a gas inlet selection and gas refining system are designed and integrated, fixed-layer sampling, selective aeration and pressurization can be realized.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganisms, in particular to a multifunctional microorganism culture device capable of layer-by-layer sampling, selective aeration, and pressurization. Background Art

[0002] The anaerobic culture of microorganisms usually requires a culture bottle or an incubator. For example, a microorganism anaerobic incubator disclosed in Patent Application No. 202221797199.1 belongs to the technical field of microorganism culture. This microorganism anaerobic incubator includes a box body and a loading platform. A negative pressure head is arranged inside the box body, and a negative pressure pipeline is arranged at the connection between the negative pressure head and the box body wall. The negative pressure head is connected to the negative pressure pipeline, and the negative pressure pipeline is connected to a negative pressure device. On both inner walls of the box body, there are foldable and telescopic storage mechanisms. Each storage mechanism consists of a bracket, a storage board, and a limiting device. One side of the storage board is provided with a foldable extension board, and a support column corresponding to the extension board is arranged on the bracket. And so on.

[0003] However, in conventional anaerobic culture, people usually cannot freely choose whether to perform gas replacement at the bottom of the culture solution or above the liquid surface. It is also difficult to evenly refine the gas at the bottom of the culture solution. At the same time, it is difficult to take into account the safety problems caused by excessive air pressure during pressurized culture. In addition, when sampling and detecting are required during the culture process, it is also impossible to freely choose to sample at the liquid surface or at the bottom of the culture solution. Therefore, it is necessary to design a multifunctional microorganism culture device capable of layer-by-layer sampling, selective aeration, and pressurization to solve the above problems. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a pressurized culture bottle capable of layer-by-layer sampling and selective aeration. This culture bottle is designed and integrated with a layer-by-layer sampling system, an air pressure stability system, an air intake selection and gas refinement system, and can perform layer-by-layer sampling, selective aeration, and pressurization.

[0005] In view of this, the technical solution of the utility model is as follows:

[0006] A pressurized culture bottle capable of layer-by-layer sampling and selective aeration, which includes a bottle body. A layer-by-layer sampling system, an air pressure stability system, and an air intake selection and gas refinement system are arranged inside the bottle body;

[0007] Among them, the layer-by-layer sampling system includes a sampling hose, a hose connector, and a hose control rod. The sampling hose extends into the bottle body and is connected to the hose connector, and the hose connector is further connected to the hose control rod. The sampling depth of the sampling hose is controlled by the hose control rod;

[0008] The air pressure stabilization system consists of an exhaust pipe and an exhaust control valve. The exhaust pipe is arranged on the bottle body and extends out of the bottle body, and an exhaust control valve is arranged thereon. By controlling the opening or closing of the exhaust control valve, pressure can be relieved in a timely manner to prevent the body of the wide-mouth bottle from bursting.

[0009] The intake selection and gas refinement system includes an intake pipe, an intake valve, and an in-bottle intake pipe. The intake valve is arranged on the intake pipe. The intake pipe is inserted into the bottle body, and a three-way valve is connected inside the bottle body. Then, it is connected to an air flotation ball and a gas refinement pipe through the three-way valve. The gas refinement pipe is arranged horizontally, and there are several small air passages on its surface for aeration through the gas refinement pipe; the air flotation ball is also connected to the in-bottle intake pipe for gas replacement through the in-bottle intake pipe.

[0010] The present utility model realizes pressurization, gas replacement, and aeration through the intake selection and gas refinement system; keep the intake open and close the exhaust to complete pressurization; close the gas refinement pipe, open the in-bottle intake pipe for gas replacement; open the gas refinement pipe and close the in-bottle intake pipe. At this time, the gas enters the bottom of the culture solution from the small air passages evenly distributed on the gas refinement pipe for aeration.

[0011] Before gas replacement, the exhaust control valve needs to be opened. After gas replacement is completed, when increasing the culture air pressure, close the exhaust control valve and continue to introduce a certain amount of gas. When pressurized culture is not required, both the intake and the exhaust can be closed simultaneously.

[0012] Furthermore, a clip is arranged on the hose to control the sampling progress. Add gas through the intake hose, then open the clip clamped on the sampling hose, and the air pressure will push the liquid out of the sampling hose. After sampling is completed, close the intake and use the clip to clamp and seal the sampling hose.

[0013] Furthermore, the layer-by-layer sampling system also includes a hose control groove and an airtight rubber. The hose control rod is arranged in the hose control groove and is sleeved with the airtight rubber. The hose control rod and the hose control groove are fixed together through the airtight rubber to facilitate controlling the depth of the sampling hose.

[0014] Even further, a depth control knob is also arranged on the hose control groove. The depth control knob is used to control the downward depth of the hose control groove. Usually, the depth control knob has internal threads, and the hose control groove has external threads. By rotating the depth control knob, the hose control groove can be lifted or lowered, so as to control the sampling depth of the sampling hose.

[0015] Furthermore, the intake selection and gas refinement system also includes an intake hose and a needle filter. The intake hose is connected to the intake pipe for facilitating intake, and the needle filter is arranged on the intake hose to control the intake filtration.

[0016] Furthermore, the intake valve is a one-way air valve that only allows intake and does not allow exhaust.

[0017] As can be seen from the above technical solutions, the embodiments of the present utility model have the following advantages:

[0018] 1. The present utility model realizes pressurization, gas replacement and aeration through the air intake selection and gas refinement system; maintaining the air intake and closing the exhaust completes pressurization; closing the gas refinement tube and opening the in-bottle intake tube for gas replacement; opening the gas refinement tube and closing the in-bottle intake tube, at this time the gas enters the bottom of the culture solution through the fine air passages evenly distributed on the gas refinement tube for aeration.

[0019] 2. The present utility model designs and integrates a layer-by-layer sampling system, a pneumatic stability system, and an air intake selection and gas refinement system, which can perform layer-by-layer sampling, selective aeration, and provide pressurization.

[0020] These features and advantages of the present utility model will be disclosed in detail in the following specific embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present utility model will be further described below with reference to the drawings:

[0022] Figure 1 It is a schematic structural diagram of the present utility model.

[0023] Description of the reference numerals in the drawings: 1. Bottle body; 2. Bottle cap; 10. Sampling hose; 11. Hose connector; 12. Hose control rod; 13. Hose control groove; 14. Depth control knob; 15. Airtight rubber; 20. Exhaust pipe; 21. Exhaust control valve; 30. Intake hose; 31. Needle filter; 32. Intake pipe; 33. Intake valve; 34. Air float ball; 35. In-bottle intake pipe; 36. Gas refinement tube; 37. Three-way valve. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] The technical solutions of the embodiments of the present utility model will be explained and described below with reference to the drawings of the embodiments of the present utility model. However, the following embodiments are only the preferred embodiments of the present utility model and not all of them. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative efforts all fall within the protection scope of the present utility model.

[0025] The following specifically describes an apparatus for targeted delivery of drugs in fecal microbiota transplantation in combination with a colonoscope with reference to the drawings of the embodiments of the present utility model.

[0026] For ease of understanding, please refer to Figure 1As shown in the figure, the pressurized culture bottle provided by the present utility model can perform layer-by-layer sampling and selective aeration. The culture bottle includes a bottle body 1, and the upper part of the bottle body 1 is covered with a bottle cap 2 for sealing. A layer-by-layer sampling system, a gas pressure stabilization system, and an air intake selection and gas refinement system are arranged in the bottle body 1.

[0027] Among them, the layer-by-layer sampling system mainly includes a sampling hose 10, a hose connector 11, and a hose control rod 12. The sampling hose 10 extends into the bottle body 1 and is connected to a hose connector 11, and the hose connector 11 is further connected to a hose control rod 12. The sampling depth of the sampling hose 10 is controlled by the hose control rod 12.

[0028] For the convenience of control, the layer-by-layer sampling system further includes a hose control groove 13, a depth control knob 14, and an airtight rubber 15. The hose control groove 13 is tubular, and the hose control rod 12 is inserted therein. The hose control rod 12 is arranged in the hose control groove 13, and its direction is restricted by the hose control groove 13. Moreover, an airtight rubber 15 is sleeved on the hose control rod 12, and the airtight rubber 15 is arranged in the hose control groove 13. The hose control rod 12 and the hose control groove 13 are fixed together through the airtight rubber 15, which is convenient for controlling the depth of the sampling hose 10.

[0029] A depth control knob 14 is further arranged on the hose control groove 13. The depth control knob 14 is located above the bottle cap 2 and is used to control the downward depth of the hose control groove 13. Usually, the depth control knob 14 has an internal thread, and the hose control groove 13 has an external thread. By rotating the depth control knob 14, the hose control groove 13 can be lifted or lowered, so as to control the sampling depth of the sampling hose 10.

[0030] The gas pressure stabilization system is composed of an exhaust pipe 20 and an exhaust control valve 21. The exhaust pipe 20 is arranged in the bottle body 1 and extends out of the bottle cap 2. An exhaust control valve 21 is arranged at the position where it extends out of the bottle cap 2, and the exhaust control valve 21 can be opened or closed by screwing. When the gas pressure is greater than a certain threshold, the film in the exhaust control valve 21 will be broken to release pressure in time, so as to avoid experimental accidents caused by the bursting of the wide-mouth bottle body.

[0031] The air intake selection and gas refinement system includes a glass intake pipe 32, an intake valve 33, a three-way valve 37, a gas floating ball 34, and an in-bottle intake pipe 35. The intake pipe 32 is inserted into the bottle body 1 and is connected to a three-way valve 37 inside the bottle body 1, and then is connected to the gas floating ball 34 and a gas refinement pipe 36 through the three-way valve 37. The gas refinement pipe 36 is arranged horizontally and has several small air channels on its surface, and aeration is carried out through the gas refinement pipe 36; the gas floating ball 34 is further connected to the in-bottle intake pipe 35, and gas replacement is carried out through the in-bottle intake pipe 35.

[0032] The intake selection and gas refinement system further includes an intake hose 30 and a needle filter 31. The intake hose 30 is connected to the intake pipe 32 to facilitate air intake. The needle filter 31 is provided on the intake hose 30 to filter and control the intake air.

[0033] The intake valve 33 is a one-way air valve provided on the intake pipe 32, which only allows air intake and does not allow air outlet.

[0034] Among them, the needle filter 31 is the same as the commonly used needle filter in the laboratory and is replaceable. The intake pipe 32 is made of glass.

[0035] Before gas replacement, the exhaust control valve 21 needs to be opened. After gas replacement, when increasing the culture air pressure, the exhaust control valve 21 needs to be closed and a certain amount of gas needs to be continuously introduced. When pressurized culture is not required, both the intake and exhaust can be closed simultaneously.

[0036] When gas replacement is selected on the liquid surface and the gas in the culture solution is not replaced, turn the three-way valve 37 to close the gas refinement tube 36 and open the intake pipe 35 inside the bottle. At this time, the air float 34 will, under the action of buoyancy, direct the intake pipe inside the bottle above the liquid surface for gas replacement. When bottom aeration of the liquid surface is selected, turn the three-way valve 37 to open the gas refinement tube 36 and close the intake pipe 35 inside the bottle. At this time, the gas enters the bottom of the culture solution through the small holes evenly distributed on the gas refinement tube 36 for aeration.

[0037] In this way, pressurization, gas replacement, and aeration are achieved through the intake selection and gas refinement system. Keeping the intake open and closing the exhaust completes pressurization. Closing the gas refinement tube and opening the intake pipe inside the bottle for gas replacement. Opening the gas refinement tube and closing the intake pipe inside the bottle. At this time, the gas enters the bottom of the culture solution through the small air channels evenly distributed on the gas refinement tube for aeration.

[0038] Among them, a clip can also be provided on the sampling hose 10 to control the sampling progress. During use, the required sampling depth is selected by rotating the depth control knob 14. Gas is added from the intake hose, and then the clip clamped on the sampling hose is opened. The air pressure will push the liquid out of the sampling hose. After sampling, the intake is closed, and the sampling hose is clamped and sealed with the clip.

[0039] In summary, the present utility model realizes pressurization, gas replacement, and aeration through the intake selection and gas refinement system. Keeping the intake open and closing the exhaust completes pressurization. Closing the gas refinement tube and opening the intake pipe inside the bottle for gas replacement. Opening the gas refinement tube and closing the intake pipe inside the bottle. At this time, the gas enters the bottom of the culture solution through the small air channels evenly distributed on the gas refinement tube for aeration.

[0040] Therefore, the utility model designs and integrates a fixed-layer sampling system, a pneumatic stability system, and an intake selection and gas refinement system, which can perform fixed-layer sampling, selective aeration, and provide pressurization.

[0041] As mentioned above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A pressurized culture bottle capable of layer - specific sampling and selective aeration, characterized in that: The culture flask includes a flask body, and a fixed-layer sampling system, a pressure stabilization system, and an air intake selection and gas refinement system are arranged inside the flask body; Among them, the fixed-layer sampling system includes a sampling hose, a hose connector, and a hose control rod. The sampling hose extends deep into the flask body and is connected to the hose connector, and the hose connector is further connected to the hose control rod. The sampling depth of the sampling hose is controlled by the hose control rod; The pressure stabilization system consists of an exhaust pipe and an exhaust control valve. The exhaust pipe is arranged on the flask body and extends out of the flask body, and an exhaust control valve is arranged thereon. The exhaust control valve is controlled to be opened or closed; The air intake selection and gas refinement system includes an air inlet pipe, an air intake valve, and an in-flask air inlet pipe. The air intake valve is arranged on the air inlet pipe. The air inlet pipe is inserted into the flask body and is connected to a three-way valve inside the flask body, and then is connected to an air float ball and a gas refinement pipe through the three-way valve. The gas refinement pipe is arranged horizontally and has a number of small air channels on its surface, and aeration is carried out through the gas refinement pipe; the air float ball is also connected to the in-flask air inlet pipe, and gas replacement is carried out through the in-flask air inlet pipe.

2. The pressurized culture flask capable of layer - defined sampling and selective aeration according to claim 1, wherein: A clip is arranged on the hose to control the sampling progress.

3. The pressurized culture bottle capable of layer - defined sampling and selective aeration according to claim 1, characterized in that: The fixed-layer sampling system further includes a hose control groove and an airtight rubber. The hose control rod is arranged in the hose control groove and is sleeved with the airtight rubber, and the hose control rod and the hose control groove are fixed together through the airtight rubber.

4. The pressurized culture flask capable of layer - defined sampling and selective aeration according to claim 3, characterized in that: A depth control knob is further arranged on the hose control groove. The depth control knob is used to control the downward depth of the hose control groove. The depth control knob has internal threads, and the hose control groove has external threads. The hose control groove is lifted or lowered by rotating the depth control knob.

5. The pressurized culture bottle capable of layer - determined sampling and selective aeration according to claim 1, wherein: The air intake selection and gas refinement system further includes an air intake hose and a needle filter. The air intake hose is connected to the air inlet pipe, and the needle filter is arranged on the air intake hose to control the intake air filtration.

6. The pressurized culture flask capable of layer - determined sampling and selective aeration according to claim 5, wherein: The air intake valve is a one-way air valve.

Images