Escherichia coli plasmid extraction device

Abstract

The escherichia coli plasmid extraction device comprises a supporting frame, a filter cartridge is embedded in the middle of the inner side of the supporting frame in a lapped and supported mode, the filter cartridge is vertically arranged, meanwhile, the upper portion of the filter cartridge is open, a liquid outlet pipe and a filter membrane arranged on the inner side of the liquid outlet pipe are arranged at the bottom of the filter cartridge, a pressing plate is arranged over the filter cartridge, and the pressing plate is connected with the filter cartridge. A lifting driving part for driving the pressing plate to move up and down is arranged at the upper end of the supporting frame, the pressing plate can seal and plug the opening in the upper portion of the filter cylinder after descending, the lifting driving part can push the pressing plate to descend and plug the opening in the upper portion of the filter cylinder, then air pressure input is conducted, and then an air pump is started; according to the plasmid extraction device, gas pressure is injected into the filter cartridge through the pipeline, under the action of the gas pressure, liquid in the filter cartridge is accelerated to settle downwards and passes through the filter membrane, so that plasmids are intercepted on the filter membrane, and meanwhile, the liquid is discharged, and compared with an existing plasmid extraction mode, the extraction efficiency is effectively improved, and high-efficiency proceeding of an experiment is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to a device for extracting Escherichia coli plasmids. Background Technique

[0002] Escherichia coli is a kind of bacterium, which generally exists in the human intestine and can play a certain role in helping digestion and regulating intestinal flora. When extracting Escherichia coli plasmids, the bacterial bodies of Escherichia coli are generally used, which can improve the activity of Escherichia coli, make the plasmids of Escherichia coli finer and easier to be absorbed by the human body, so as to achieve the effect of regulating intestinal flora.

[0003] In the prior art, in order to purify Escherichia coli plasmids (also known as plasmid extraction), a filtration device is usually used to screen and extract Escherichia coli plasmids in the stock solution. However, the existing screening and filtering methods for extracting Escherichia coli plasmids are relatively inefficient, and it takes a long time to complete the screening and filtering of the stock solution, especially when extracting a large-capacity stock solution; in addition, a centrifuge is also used for centrifugal extraction, but the internal maintenance and cleaning of the centrifuge are relatively troublesome after each use. Therefore, there is an urgent need for a device for extracting Escherichia coli plasmids to solve the above problems. Summary of the Utility Model

[0004] The purpose of this part is to outline some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. In this part, as well as in the abstract and the title of the utility model of this application, some simplifications or omissions may be made to avoid obscuring the purpose of this part, the abstract of the specification and the title of the utility model, and such simplifications or omissions shall not be used to limit the scope of the utility model.

[0005] Therefore, the purpose of the utility model is to provide a device for extracting Escherichia coli plasmids to solve the problems in the prior art that the device for extracting Escherichia coli plasmids has low efficiency and it takes a long time to complete the screening and filtering of the stock solution; and the internal maintenance and cleaning of the centrifuge are relatively troublesome after each use.

[0006] To achieve the above purpose, the utility model provides the following technical solution: a device for extracting Escherichia coli plasmids, which includes a support frame. A filter cylinder is fitted and supported in the middle inside the support frame, and the filter cylinder is vertically arranged. At the same time, the upper part of the filter cylinder is open. The bottom of the filter cylinder has a liquid outlet pipe and a filter membrane arranged inside the liquid outlet pipe. A pressing plate is arranged directly above the filter cylinder. A lifting drive component for driving the pressing plate to move up and down is arranged at the upper end of the support frame. After the pressing plate descends, it can seal and block the open upper part of the filter cylinder. An air delivery device for injecting positive air pressure into the inner cavity of the filter cylinder is also connected to the upper part of the pressing plate.

[0007] As a preferred embodiment of the Escherichia coli plasmid extraction device of the present utility model, on both sides of the inner wall of the support frame, there are also positioning seats. On the outer peripheral side of the top of the filter cartridge, there are ear rings, and on both sides of the bottom of the ear rings, there are pin rods vertically inserted into the positioning seats.

[0008] As a preferred embodiment of the Escherichia coli plasmid extraction device of the present utility model, on the inner peripheral side of the liquid outlet pipe, there is also a limit step groove corresponding to the filter membrane, and the filter membrane is fitted into the inside of the liquid outlet pipe from top to bottom.

[0009] As a preferred embodiment of the Escherichia coli plasmid extraction device of the present utility model, the lifting drive component is an electric push cylinder, and the output shaft at the bottom of the electric push cylinder is connected to the top of the pressing plate. A sealing ring is also provided around the bottom of the pressing plate.

[0010] As a preferred embodiment of the Escherichia coli plasmid extraction device of the present utility model, on one side of the top of the pressing plate, there is an air nozzle;

[0011] The air delivery device includes an air pump provided at the upper end of the support frame, an air delivery pipe connected to the output end of the air pump, and an air needle plug located at the other end of the air delivery pipe and inserted into the inside of the air nozzle.

[0012] As a preferred embodiment of the Escherichia coli plasmid extraction device of the present utility model, a base is further fixed at the bottom of the support frame, and a liquid collection bucket is placed on the upper end of the base and located below the filter cartridge.

[0013] Compared with the prior art, the beneficial effects of the present utility model are:

[0014] (1) For this Escherichia coli plasmid extraction device, after injecting the stock solution into the filter cartridge, the lifting drive component will push the pressing plate down to seal the upper open end of the filter cartridge, so as to subsequently input air pressure. Then, the air pump is turned on, so that the gas pressure is injected into the filter cartridge through the pipeline. Under the action of the air pressure, the liquid in the filter cartridge will accelerate downward settlement and pass through the filter membrane, so that the plasmid is retained on the filter membrane while the liquid is discharged. Compared with the existing plasmid extraction methods, the extraction efficiency is effectively improved, which is beneficial to the efficient progress of the experiment.

[0015] (2) For this Escherichia coli plasmid extraction device, through the cooperation of the positioning seat, ear ring, pin rod, liquid outlet pipe, etc., the rapid installation and disassembly of the filter cartridge and the filter membrane can be realized, which is beneficial to the cleaning and replacement and maintenance of the screening and filtering components. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall external structure of the present utility model;

[0017] Figure 2Schematic diagram of the internal structure of the filter cartridge of the present utility model;

[0018] Figure 3 Schematic diagram of the structure of the gas transmission device of the present utility model.

[0019] In the figure: 100, support frame; 110, positioning seat; 120, base; 200, filter cartridge; 210, liquid outlet pipe; 211, limiting step groove; 220, filter membrane; 230, ear ring; 240, pin rod; 300, pressing plate; 310, sealing ring; 320, air nozzle; 400, lifting drive component; 500, gas transmission device; 510, air pump; 520, gas transmission pipe; 530, air needle plug; 600, liquid collection bucket. Specific embodiments

[0020] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the specific embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

[0021] Secondly, the present utility model will be described in detail in combination with the schematic diagrams. When describing the embodiments of the present utility model in detail, for the convenience of description, the cross-sectional views showing the device structure will be enlarged locally in a non-general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present utility model herein. In addition, in actual production, three-dimensional spatial dimensions including length, width, and depth should be included.

[0022] To make the purpose, technical solution, and advantages of the present utility model clearer, the embodiments of the present utility model will be further described in detail below with reference to the accompanying drawings.

[0023] Figures 1-3 Shown is the schematic diagram of the entire structure of an Escherichia coli plasmid extraction device of the present utility model. Please refer to Figures 1-3 , An Escherichia coli plasmid extraction device in this embodiment includes a support frame 100. A filter cartridge 200 is fitted and supported in the middle of the inner side of the support frame 100, and the filter cartridge 200 is vertically arranged. At the same time, the upper part of the filter cartridge 200 is open. The bottom of the filter cartridge 200 has a liquid outlet pipe 210 and a filter membrane 220 arranged inside the liquid outlet pipe 210. A pressing plate 300 is arranged directly above the filter cartridge 200. An upper end of the support frame 100 is provided with a lifting drive component 400 for driving the pressing plate 300 to move up and down. After the pressing plate 300 descends, it can seal and block the upper open end of the filter cartridge 200. The upper part of the pressing plate 300 is also connected with a gas transmission device 500 for injecting positive air pressure into the inner cavity of the filter cartridge 200.

[0024] On both sides of the inner wall of the support frame 100, there are also positioning seats 110. On the outer peripheral side of the top of the filter cartridge 200, there are ear rings 230. On both sides of the bottom of the ear rings 230, there are pin rods 240 vertically inserted on the positioning seats 110. During use, directly place the ear rings 230 on both sides of the filter cartridge 200 on the positioning seats 110, and insert the pin rods 240 on the positioning seats 110 and the support frame 100, so as to stabilize the filter cartridge 200, making the pressure plate 300 and the filter cartridge 200 vertically corresponding. When disassembly or cleaning is required, the staff can directly lift the filter cartridge 200 upwards, and the operation is simple. On the inner peripheral side of the liquid outlet pipe 210, there is also a limit step groove 211 corresponding to the filter membrane 220, and the filter membrane 220 is fitted into the inner side of the liquid outlet pipe 210 from top to bottom. When the filter membrane 220 needs to be disassembled and replaced, just push out the filter membrane 220 from the bottom of the liquid outlet pipe 210, and then reach into the inner side of the filter cartridge 200 to take out and synchronously replace the filter membrane 220. The entire structure of the filter cartridge 200 is simple and easy to operate, and the cleaning is convenient.

[0025] The lifting drive component 400 is an electric push cylinder, and the output shaft at the bottom of the electric push cylinder is connected to the top of the pressure plate 300. There is also a sealing ring 310 around the bottom circumference of the pressure plate 300. With the cooperation of the sealing ring 310, the pressure plate 300 can tightly seal the upper opening of the filter cartridge 200, avoiding air leakage during use. And both the sealing pressing and detachment operations are performed by the electric push cylinder, which is convenient to use. On one side of the top of the pressure plate 300, there is an air nozzle 320; the gas supply device 500 includes an air pump 510 arranged at the upper end of the support frame 100, an air delivery pipe 520 connected to the output end of the air pump 510, and an air needle plug 530 located at the other end of the air delivery pipe 520 and inserted into the inner side of the air nozzle 320. Through such a design, it is convenient to disassemble and connect between the air delivery pipe 520 and the pressure plate 300. Specifically, during use, after injecting the original liquid into the filter cartridge 200, the lifting drive component 400 will push the pressure plate 300 down to seal the upper opening of the filter cartridge 200 for subsequent air pressure input. Then, turn on the air pump 510, so that the gas pressure is injected into the filter cartridge 200 through the pipeline. Under the action of the air pressure, the liquid in the filter cartridge 200 will accelerate downward settlement and pass through the filter membrane 220, so that the plasmid is retained on the filter membrane 220 while the liquid is discharged. Compared with the existing plasmid extraction method, the extraction efficiency is effectively improved, which is beneficial to the efficient progress of the experiment.

[0026] Furthermore, a base 120 is fixed at the bottom of the support frame 100. A liquid collection bucket 600 is placed on the upper end of the base 120 and is located below the filter cartridge 200. It can be understood that the entire extraction device can be stabilized through the arranged base 120, and the liquid extracted and seeped through the filter cartridge 200 can be collected through the placed liquid collection bucket 600 to prevent tabletop pollution.

[0027] In summary, for an Escherichia coli plasmid extraction device according to this embodiment, during use, after injecting the original solution into the filter cartridge 200, the lifting drive component 400 will push the pressing plate 300 downward to seal the upper open end of the filter cartridge 200, so as to subsequently input air pressure. Then, the air pump 510 is turned on, so that the gas pressure is injected into the filter cartridge 200 through the pipeline. Under the action of the air pressure, the liquid in the filter cartridge 200 will accelerate downward settlement and pass through the filter membrane 220, so that the plasmid is retained on the filter membrane 220 while the liquid is discharged. Compared with the existing plasmid extraction methods, the extraction efficiency is effectively improved.

[0028] Although the present invention has been described above with reference to the embodiments, various improvements can be made to it and components therein can be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention can be combined with each other in any way. The exhaustive description of these combinations is not given in this specification only for the sake of saving space and resources. Therefore, the present invention is not limited to the specific embodiments disclosed in the text, but includes all technical solutions falling within the scope of the claims.

Claims

1. An Escherichia coli plasmid extraction device, characterized in that, It includes a support frame (100). In the middle of the inner side of the support frame (100), a filter cartridge (200) is fitted and supported. The filter cartridge (200) is vertically arranged, and the upper part of the filter cartridge (200) is open. The bottom of the filter cartridge (200) has a liquid outlet pipe (210) and a filter membrane (220) arranged inside the liquid outlet pipe (210). A pressing plate (300) is arranged directly above the filter cartridge (200). An elevating drive component (400) for driving the pressing plate (300) to move up and down is arranged at the upper end of the support frame (100). After the pressing plate (300) descends, it can seal and block the open upper part of the filter cartridge (200). A gas transmission device (500) which is connected to the upper part of the pressing plate (300) and is used to inject positive air pressure into the inner cavity of the filter cartridge (200) is also provided.

2. The Escherichia coli plasmid extraction device according to claim 1, wherein: On both sides of the inner wall of the support frame (100), there are also positioning support seats (110). On the outer peripheral side of the top of the filter cartridge (200), there are ear rings (230). On both sides of the bottom of the ear rings (230), there are pin rods (240) vertically inserted and embedded on the positioning support seats (110).

3. An Escherichia coli plasmid extraction device according to claim 1, characterized in that: On the inner peripheral side of the liquid outlet pipe (210), there is also a limiting step groove (211) corresponding to the filter membrane (220), and the filter membrane (220) is fitted and embedded inside the liquid outlet pipe (210) from top to bottom.

4. The Escherichia coli plasmid extraction device according to claim 1, wherein: The elevating drive component (400) is an electric push cylinder, and the output shaft at the bottom of the electric push cylinder is connected to the top of the pressing plate (300). A sealing ring (310) is also arranged in a circle around the bottom of the pressing plate (300).

5. The Escherichia coli plasmid extraction device according to claim 1, characterized in that: On one side of the top of the pressing plate (300), there is a gas nozzle (320); The gas transmission device (500) includes an air pump (510) arranged at the upper end of the support frame (100), an air transmission pipe (520) connected to the output end of the air pump (510), and an air needle plug (530) located at the other end of the air transmission pipe (520) and inserted and embedded inside the gas nozzle (320).

6. The Escherichia coli plasmid extraction device according to claim 1, wherein: A base (120) is also fixed at the bottom of the support frame (100). A liquid collection bucket (600) which is placed at the upper end of the base (120) and is located below the filter cartridge (200) is provided.

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