PHA (polyhydroxyalkanoate) supplementing system capable of preventing microbiological contamination

Abstract

The utility model discloses a PHA (polyhydroxyalkanoate) supplementing system capable of preventing microbiological contamination, which is characterized in that a batching tank is connected with a supplementing tank, and the supplementing tank is respectively connected with a seed tank, a fermentation tank and a cleaning water storage tank through pipelines; a coil pipe is arranged in the material supplementing tank, one end of the coil pipe is connected with the steam generator, and the other end of the coil pipe is connected with the condensate water storage tank. According to the utility model, the problem in the material supplementing tank is solved through the coil pipe, the temperature of materials is increased and is kept above 60 DEG C, the sterilization and the inhibition of the growth of infectious microbes are synchronously realized, and the contamination risk of a fermentation system is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of fermentation sterilization, and particularly relates to a PHA feeding system for preventing bacteria contamination. Background Technique

[0002] PHA (polyhydroxyalkanoate) is a general term for high molecular weight polyesters produced by microorganisms using a variety of carbon sources. Due to its good biocompatibility, biodegradability and thermoplastic processing properties, it can be used as an environmentally friendly alternative to traditional petroleum-based plastics, and thus is widely used in fields such as packaging materials, medical supplies, and agricultural films. At present, the production process of PHA mainly includes microbial fermentation and chemical synthesis methods. Among them, microbial fermentation technology has been able to significantly reduce the complexity and cost of downstream processing through strain breeding, modification and fermentation technology optimization, so it has also become the mainstream production process of PHA.

[0003] In the process of microbial fermentation, it is necessary to strictly control the invasion of miscellaneous bacteria into the fermentation system, which will make the fermentation process lose the true meaning of pure culture, resulting in non-target fermentation products or excessive impurities and complex components in the fermentation products, bringing great pressure to the extraction and purification process. Therefore, in the traditional fermentation process, it is necessary to perform high-temperature sterilization on the fermentation tank and raw materials, and then carry out fermentation and feeding. At present, for soluble fermentation raw materials, they are generally mixed with water to form a suspension, and then sterilized by high temperature and high pressure or chemical disinfectants. However, this method has problems such as long processing time, complex operation, high energy consumption, and partial loss; when solid materials with poor solubility or insoluble materials are mixed with water, they tend to form agglomerates, and it is difficult to control the size of the agglomerates, so it is also difficult to achieve thorough sterilization, and there is a risk of fermentation system contamination and fermentation failure due to incomplete sterilization. In addition, there is also a risk of recontamination of the sterilized materials during storage due to improper storage.

[0004] Chinese Patent CN106075508A discloses a sterilization method and device for a feeding pipe of a multi-connected fermentation tank, including an ethanol washing bottle, a sodium hydroxide washing bottle, and a pure water washing bottle respectively provided with a liquid infusion pipe; it also includes a pressure adjustment device for adjusting the air pressure in the ethanol washing bottle, sodium hydroxide washing bottle and pure water washing bottle to make the liquid in each washing bottle flow out smoothly, and a collection bottle for storing waste liquid. Specifically, the feeding pipe is rinsed with 75% by volume ethanol solution, 2mol / L sodium hydroxide solution and pure water in sequence. The ethanol solution, sodium hydroxide solution and pure water can be provided with a circulation power by a peristaltic pump of the multi-connected fermentation tank, avoiding pre-treatment operations such as disassembly and installation of each feeding pipe before sterilization, and reducing the risk of bacteria contamination.

[0005] Chinese patent CN117720999A discloses a continuous sterilization and feeding device for fermentation of sterile solid-state feed, comprising a grinder, a high-temperature instantaneous sterilization main unit, a cooling and conveying structure, a feeding structure, and a system pipeline dust removal structure; the grinder, the high-temperature instantaneous sterilization main unit, the cooling and conveying structure, and the feeding structure are sealed and connected in sequence, and the carbon source is input from the grinder to the fermentation tank through the above-mentioned structures connected in sequence; the carbon source enters the fermentation tank through the material temporary storage bin; the system pipeline dust removal structure uses an air lock to settle the dust generated during the use of the device. Such an interconnected closed system avoids the defects of manual operation and intermittent feeding, and improves the stability of the fermentation process and the quality of the product. By optimizing the sterilization and feeding process and recycling the dust generated during use, the control system cooperates with the relevant pneumatic valve structure to automatically complete the dust removal and sterilization functions of the system in a closed state, reducing energy consumption and resource consumption, and reducing the impact on the environment.

[0006] At present, the sterilization of materials still uses high temperature or chemical reagents for disinfection, and the sterility is maintained by controlling the sealing of equipment and pipelines. This has high requirements for equipment, and the sterilization methods and sterilization devices provided can only be applied to a specific fermentation situation, and the scope of application is relatively narrow. Summary of the Invention

[0007] In response to the above technical problems, the utility model provides a PHA feeding system that prevents contamination. The system uses a coil to increase the temperature of the feeding tank, raise the temperature of the material and keep it above 60°C, thereby simultaneously achieving sterilization and inhibiting the growth of miscellaneous bacteria, and reducing the risk of contamination in the fermentation system.

[0008] In order to achieve the above-mentioned purpose, the utility model provides a PHA feeding system that prevents contamination, wherein the feed tank is connected to the feeding tank, and the feeding tank is respectively connected to the seed tank, the fermentation tank and the cleaning water storage tank pipeline; a coil is provided inside the feeding tank, one end of the coil is connected to the steam generator, and the other end is connected to the condensed water storage tank.

[0009] Preferably, a high-position tuning fork, a middle-position tuning fork and a low-position tuning fork are provided on the outside of the feeding tank.

[0010] Preferably, the condensed water storage tank is connected to the wash water storage tank, and the wash water storage tank is connected to the rotary spray head pipeline.

[0011] Further preferably, the rotary spray head is located inside the feeding tank.

[0012] Preferably, a stirring device is provided inside the batching tank, and the stirring device is connected to a stirring motor.

[0013] Preferably, the top of the batching tank is connected to the material storage tank and the process water storage tank pipeline respectively.

[0014] Further preferably, the process water storage tank is connected to the cleaning water storage tank through a pipeline.

[0015] The beneficial effects of the present utility model are as follows:

[0016] 1. A coil pipe is arranged inside the feeding tank, and then steam is introduced into the coil pipe to raise the overall temperature inside the feeding tank. The temperature is increased according to the need for sterilization, and the steam consumption is regulated during storage to maintain the temperature inside the feeding tank at about 60°C, inhibiting the growth of miscellaneous bacteria, enabling the material to be in a sterile state for a long time, and reducing the risk of bacterial contamination. In addition, when the heated material enters the seed tank and / or fermentation tank, it can increase the temperature of the tank body, reduce the heating energy consumption of the seed tank and / or fermentation tank, and at the same time avoid the material with a lower temperature entering the seed tank and / or fermentation tank with a higher temperature, which has a greater impact on the fermentation temperature, keeping the fermentation running smoothly all the time, and improving the fermentation efficiency.

[0017] 2. The present utility model is applicable to various material types, and can simultaneously achieve the feeding and sterilization of multiple materials through series connection. It has a simple structure, good effect of preventing bacterial contamination, can appropriately reduce the sterilization energy consumption, and avoid the impact on fermentation caused by bacterial contamination of the material. Description of the Drawings

[0018] Figure 1 It is a schematic diagram of the overall structure of the present utility model. In the figure, 1 is the material storage tank, 2 is the process water storage tank, 3 is the stirring motor, 4 is the batching tank, 5 is the stirring device, 6 is the washing water storage tank, 7 is the rotating spray head, 8 is the high-level tuning fork, 9 is the feeding tank, 10 is the middle-level tuning fork, 11 is the low-level tuning fork, 12 is the condensate storage tank, 13 is the steam generator, 14 is the coil pipe, 15 is the fermentation tank, 16 is the seed tank, and 17 is the cleaning water storage tank. Detailed Embodiments

[0019] The technical solutions of the present utility model will be further explained and illustrated below in conjunction with the drawings and specific embodiments. It should be noted that the following embodiments are only the preferred embodiments of the present utility model, and should not be construed as a limitation to the present utility model. The protection scope of the present utility model shall be subject to the content recorded in the claims. Any modification and replacement made by those skilled in the art to the technical solutions of the present utility model without creative work fall within the protection scope of the present utility model.

[0020] Embodiment 1

[0021] As Figure 1As shown, a PHA feeding system for preventing contamination is characterized in that: the feed tank 4 is connected to the feeding tank 9, and the feeding tank 9 is respectively connected to the seed tank 16, the fermentation tank 15 and the cleaning water storage tank 17 through pipelines; a coil 14 is provided inside the feeding tank 9, and one end of the coil 14 is connected to the steam generator 13. The steam generator 13 transports steam to the inside of the feeding tank 9 through the coil 14 to heat the material therein; the other end of the coil 14 is connected to the condensed water storage tank 12, and the condensed water is output and stored in the condensed water storage tank 12 for use in the next process; a valve is provided on the pipeline connecting the steam generator 13 and the coil 14 to adjust the amount of steam introduced; a check valve is provided on the pipeline connecting the coil 14 and the condensed water storage tank 12 to prevent the condensed water from returning to the coil and affecting the heating effect.

[0022] Preferably, a high-position tuning fork 8 , a middle-position tuning fork 10 and a low-position tuning fork 11 are provided on the outside of the feeding tank 9 to detect the amount of material in the feeding tank 9 .

[0023] Preferably, the condensed water storage tank 12 is connected to the washing water storage tank 6, and the washing water storage tank 6 is connected to the rotary spray head 7 pipeline. After the condensed water and the washing water are merged, they are input into the feeding tank 9 through the rotary spray head 7 to achieve the cleaning of the feeding tank 9. The cleaning water enters the washing water storage tank 17 through the discharge port at the bottom of the feeding tank 9 for storage, and then is transported to the process water storage tank 2 through the pipeline for the material batching tank 2 to dilute and concentrate the material.

[0024] Further preferably, the rotary spray head 7 is located inside the feeding tank 9 to achieve cleaning of the inside of the feeding tank 9.

[0025] Preferably, a stirring device 5 is provided inside the batching tank 4, and the stirring device is connected to the stirring motor 3 to stir, mix or disperse the materials in the batching tank.

[0026] Preferably, the top of the batching tank 4 is connected to the material storage tank 1 and the process water storage tank 2 through pipelines, and valves are provided on the pipelines to selectively input materials and process water into the batching tank 4 for batching.

[0027] Further preferably, the process water storage tank 2 is connected to the cleaning water storage tank 17 via a pipeline, so that the cleaning water in the cleaning water storage tank 17 is recycled to improve the utilization rate of water resources.

[0028] Example 2

[0029] When sterilizing solid materials: The solid materials in the material storage tank 1 are transported to the batching tank 4, and are stirred and dispersed by the stirring motor and the stirring device. Then, the dispersed solid materials are transported to the feeding tank 9 through pipelines. The valve is opened to transport the steam in the steam generator 13 to the coil 14, and the coil 14 is used to heat up the materials. First, the temperature is raised to above 95°C and maintained for 30 minutes for sterilization. Then, the steam input amount is reduced through the valve to keep the temperature in the feeding tank 9 at 60°C ± 5°C to prevent bacterial contamination. When the seed tank 16 and / or the fermentation tank 15 need to be fed, the valve is opened to transport the materials in the feeding tank 9 to the seed tank 16 and / or the fermentation tank 15 to complete the feeding.

[0030] When sterilizing liquid materials: The solid materials in the material storage tank 1 and the process water in the process water storage tank 2 are transported to the batching tank 4, and are stirred and mixed by the stirring motor and the stirring device. The mixed liquid materials are transported to the feeding tank 9 through pipelines. The valve is opened to transport the steam in the steam generator 13 to the coil 14, and the coil 14 is used to heat up the materials. First, the temperature is raised to above 95°C and maintained for 30 minutes for sterilization. Then, the steam input amount is reduced through the valve to keep the temperature in the feeding tank 9 at 60°C ± 5°C to prevent bacterial contamination. When the seed tank 16 and / or the fermentation tank 15 need to be fed, the valve is opened to transport the materials in the feeding tank 9 to the seed tank 16 and / or the fermentation tank 15 to complete the feeding.

Claims

1. A PHA feeding system for preventing contamination, characterized by: The feed tank (4) is connected to the feed tank (9), and the feed tank (9) is respectively connected to the seed tank (16), the fermentation tank (15) and the cleaning water storage tank (17) through pipelines; a coil (14) is provided inside the feed tank (9), one end of the coil (14) is connected to the steam generator (13), and the other end is connected to the condensed water storage tank (12).

2. A PHA feeding system for preventing contamination according to claim 1, characterized in that: A high-position tuning fork (8), a middle-position tuning fork (10) and a low-position tuning fork (11) are provided outside the feeding tank (9).

3. A PHA feeding system for preventing contamination according to claim 1, characterized in that: The condensed water storage tank (12) is connected to the wash water storage tank (6), and the wash water storage tank (6) is connected to the rotary spray head (7) pipeline.

4. A PHA feeding system for preventing contamination according to claim 3, characterized in that: The rotating spray head (7) is located inside the feeding tank (9).

5. The PHA feeding system for preventing contamination according to claim 1, characterized in that: A stirring device (5) is provided inside the mixing tank (4), and the stirring device is connected to the stirring motor (3).

6. A PHA feeding system for preventing contamination according to claim 1, characterized in that: The top of the batching tank (4) is respectively connected to the pipelines of the material storage tank (1) and the process water storage tank (2).

7. A PHA feeding system for preventing contamination according to claim 6, characterized in that: The process water storage tank (2) is connected to the cleaning water storage tank (17) through a pipeline.

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