Sterile sampling and harvesting device for reactor

Abstract

The utility model relates to a reactor sterile sampling and harvesting device which comprises a liquid outlet pipe used for being connected with a reactor, the end, away from the reactor, of the liquid outlet pipe is connected with a tee joint A, the other two ends of the tee joint A are connected with a first guide pipe and a second guide pipe respectively, and the end, away from the tee joint A, of the first guide pipe is connected with a harvesting quick connector. The other two ends of the tee joint B are connected with a third guide pipe and a fourth guide pipe respectively, the end, away from the tee joint B, of the third guide pipe is connected with a sampling quick connector, and the end, away from the tee joint B, of the fourth guide pipe is connected with an air filtering device. The device further comprises a clamping tool. The harvesting end is separated from the sampling end, so that the quick connector is prevented from repeatedly connecting the harvesting barrel and the sampling bottle, and the pollution risk is reduced; after sampling is completed, a new sampling bottle can be replaced, the sampling bottle can be directly opened for next sampling, and the link of waiting for cooling of the quick connector is avoided; by utilizing an air filtering device and a clamping tool, residual liquid in the pipeline is discharged, and the influence on next sampling is avoided.

Description

Technical Field

[0001] The present application relates to the technical field of experimental equipment, and in particular to a sterile sampling and harvesting device for a reactor. Background Art

[0002] A bioreactor is a device that provides a suitable environment for the proliferation of living cells and the biochemical reactions within them. It is a key piece of equipment in the biopharmaceutical process. It can effectively meet the needs of cell survival and high-density proliferation, thus providing a foundation for the production of related vaccines.

[0003] However, in the process of producing viral vaccines (such as hand, foot and mouth disease virus vaccine), there are the following problems with sampling and harvesting of reactor perfusion culture: First, if a sample is taken at a certain time point, it is necessary to disconnect the harvest barrel and connect the reactor to the sampling bottle. After the sampling is completed, the sampling bottle is disconnected and the reactor is connected to the harvest barrel. This operation is not only cumbersome, but also prone to contamination during multiple connections; second, each time the quick-connect connector is burned at high temperature with an alcohol lamp, it must be cooled before sampling can be performed, and the sampling efficiency is low; third, after each sampling is completed, liquid may remain in the sampling pipeline, and the residual liquid will affect the sampling results when the next sampling is taken. Utility Model Content

[0004] In order to improve the above-mentioned problems existing in the existing reactor perfusion culture sampling and harvesting, the present application provides a reactor aseptic sampling and harvesting device.

[0005] The present application provides a reactor aseptic sampling and harvesting device that adopts the following technical solutions:

[0006] A sterile sampling and harvesting device for a reactor comprises a liquid outlet pipe for connecting to a reactor, wherein one end of the liquid outlet pipe away from the reactor is connected to a tee A, the other two ends of the tee A are respectively connected to a catheter 1 and a catheter 2, the end of the catheter 1 away from the tee A is connected to a harvesting quick connector, the end of the catheter 2 away from the tee A is connected to a tee B, the other two ends of the tee B are respectively connected to a catheter 3 and a catheter 4, the end of the catheter 3 away from the tee B is connected to a sampling quick connector, and the end of the catheter 4 away from the tee B is connected to an air filter device; and the device also comprises a clamping tool.

[0007] By adopting the above technical solution, the harvesting end and the sampling end are separated from each other, which can avoid the repeated connection of the quick connector to the harvesting bucket and the sampling bottle, reducing the risk of contamination, and the operation is relatively simple; after the sampling is completed, a new sampling bottle can be replaced immediately, and the next sampling can be opened directly, avoiding the step of waiting for the quick connector to cool down, thereby improving the sampling efficiency; using an air filter device and a clamping tool, the residual liquid in the pipeline can be discharged to avoid affecting the next sampling.

[0008] Optionally, the first conduit, the second conduit, the third conduit, and the fourth conduit are all flexible hoses.

[0009] By adopting the above technical solution, the flexible hoses are easy to bend and rotate, facilitating the bending and rotation of the first conduit, the second conduit, the third conduit, and the fourth conduit according to requirements.

[0010] Optionally, the clamping tool is a hemostat.

[0011] By adopting the above technical solution, the hemostat is easy to operate and has a good use effect.

[0012] Optionally, the harvesting quick connector is a harvesting quick connector made of metal, and the sampling quick connector is a sampling quick connector made of metal.

[0013] By adopting the above technical solution, the harvesting quick connector and the sampling quick connector have higher hardness, facilitating the insertion and connection with the corresponding barrel or bottle body.

[0014] Optionally, the air filtering device is an air filter membrane.

[0015] By adopting the above technical solution, the air filter membrane is light in weight and can meet the use requirements.

[0016] Optionally, among the first conduit, the second conduit, the third conduit, and the fourth conduit, the second conduit has the shortest length.

[0017] By adopting the above technical solution, the second conduit has a shorter length to reduce liquid residue.

[0018] Optionally, the fourth conduit is detachably connected to the air filtering device.

[0019] By adopting the above technical solution, it is convenient to repair or replace the air filtering device.

[0020] Optionally, a fifth conduit is tightly inserted at one end of the fourth conduit away from the three-way joint B, and an air filtering device is fixedly connected to one end of the fifth conduit away from the fourth conduit.

[0021] By adopting the above technical solution, pulling the fifth conduit in the direction away from the fourth conduit until the fifth conduit is disengaged from the insertion with the fourth conduit, at this time, it is convenient to repair or replace the air filtering device on the fifth conduit, and the operation is simple and convenient.

[0022] In summary, the present application includes at least one of the following beneficial technical effects:

[0023] 1. The harvesting end and the sampling end are separated from each other, which can avoid the repeated connection of the quick connector to the harvesting bucket and the sampling bottle, reduce the contamination risk, and at the same time the operation is relatively simple; after the sampling is completed, a new sampling bottle can be replaced immediately, and the next sampling can be directly started, avoiding the link of waiting for the quick connector to cool down, and improving the sampling efficiency; by using an air filtration device and a clamping tool, the residual liquid in the pipeline is discharged to avoid affecting the next sampling.

[0024] 2. The haemostat is easy to operate and has good use effect.

[0025] 3. The length of the second catheter is shorter to reduce liquid residue. Description of the Drawings

[0026] Figure 1 It is a schematic structural diagram of the reactor aseptic sampling and harvesting device in the first embodiment of the present application.

[0027] Figure 2 It is a schematic structural diagram showing the fifth catheter in the second embodiment of the present application.

[0028] Description of the reference numerals: 1, reactor; 10, liquid outlet pipe; 2, tee A; 20, first catheter; 21, second catheter; 3, harvesting quick connector; 4, tee B; 40, third catheter; 41, fourth catheter; 5, sampling quick connector; 6, air filter membrane; 7, fifth catheter. Detailed Description of the Embodiment

[0029] The following is a further detailed description of the present application in conjunction with the attached Figure 1 - Figure 2 drawings.

[0030] Embodiment 1

[0031] The embodiment of the present application discloses a reactor aseptic sampling and harvesting device. Refer to Figure 1 , a reactor aseptic sampling and harvesting device includes a reactor 1, and the reactor 1 is placed on a table. A liquid outlet pipe 10 is connected to the top of the reactor 1, the liquid outlet pipe 10 is a flexible pipe, and a tee A 2 is connected to the end of the liquid outlet pipe 10 far from the reactor 1. The other two ends of the tee A 2 are respectively connected to a first catheter 20 and a second catheter 21. Both the first catheter 20 and the second catheter 21 are flexible pipes. The length of the second catheter 21 is less than the length of the first catheter 20. The first catheter 20 and the second catheter 21 are cooperated with a clamping tool, for example, a haemostat is used to clamp and seal the catheter.

[0032] Refer to Figure 1, at one end of the conduit 1 20 away from the three-way joint A2, a harvesting quick connector 3 is connected. The harvesting quick connector 3 is a metal head and is used to connect with a harvesting bucket, and the harvesting bucket is placed on the ground. At one end of the conduit 2 21 away from the three-way joint A2, a three-way joint B4 is connected. The other two ends of the three-way joint B4 are respectively connected with a conduit 3 40 and a conduit 4 41. The lengths of the conduit 3 40 and the conduit 4 41 are both greater than the length of the conduit 2 21. The conduit 3 40 and the conduit 4 41 are both flexible hoses, and the conduit 3 40 and the conduit 4 41 are equipped with clamping tools, for example, a hemostat is used to clamp and seal the conduit.

[0033] Refer to Figure 1 , at one end of the conduit 3 40 away from the three-way joint B4, a sampling quick connector 5 is connected. The sampling quick connector 5 is a metal head and is used to connect with a sampling bottle, and the sampling bottle is placed on the table; at one end of the conduit 4 41 away from the three-way joint B4, an air filter membrane 6 is connected. The air filter membrane 6 is used to filter impurities in the air. In other embodiments, other structures with air filtering functions can also be used as the air filtering device.

[0034] The implementation principle of a reactor aseptic sampling and harvesting device in an embodiment of the present application is as follows: The harvesting end and the sampling end are separately arranged, and sampling and harvesting do not interfere with each other, avoiding repeated connection of the harvesting bucket and the sampling bottle using the same quick connector, reducing the pollution risk, and at the same time, the operation is relatively simple.

[0035] When harvesting is required, the experimenter uses a hemostat to clamp the conduit 2 21. After the liquid is output from the reactor 1, it successively passes through the liquid outlet pipe 10, the three-way joint A2, the conduit 1 20, and the harvesting quick connector 3 and then enters the harvesting bucket; then, the hemostat on the conduit 2 21 is removed, and the hemostat is clamped on the conduit 3 40. The conduit 2 21, the three-way joint B4, the conduit 4 41, and the air filter membrane 6 are lifted, and the residual liquid in the conduit 2 21 and the conduit 1 20 is discharged by gravity to achieve the purpose of removing the residual liquid.

[0036] When sampling is required, the staff uses a hemostat to clamp the conduit 1 20 and the conduit 4 41. After the liquid is output from the reactor 1, it successively passes through the liquid outlet pipe 10, the three-way joint A2, the conduit 2 21, the three-way joint B4, the conduit 3 40, and the sampling quick connector 5 and then enters the sampling bottle; then, the hemostat on the conduit 4 41 is removed, and the conduit 4 41 and the air filter membrane 6 are lifted, and the residual liquid in the conduit 3 40 is discharged by gravity. The residual liquid in the conduit 2 21 can be cleared according to the cleaning method during harvesting to achieve the purpose of removing the residual liquid.

[0037] Before connecting the sampling bottle or the harvesting bucket, the experimenter needs to use an alcohol lamp to burn the sampling quick connector 5 or the harvesting quick connector 3 for high-temperature sterilization. Taking the connection of the sampling bottle as an example, during the first connection process, the experimenter first burns the sampling quick connector 5. After the sampling quick connector 5 cools down, the sampling work can be carried out. Since there is a certain time interval for the sampling work, after the first sampling is completed, the first sampling bottle is removed. Then, the sampling quick connector 5 is burned and the second sampling bottle is connected. When the second sampling is needed, the sampling quick connector 5 has already cooled down and the sampling work can be carried out immediately without waiting, saving the experiment time and improving the experiment efficiency.

[0038] Embodiment 2

[0039] This application embodiment discloses a reactor aseptic sampling and harvesting device. Referring to Figure 2 , different from Embodiment 1, in order to facilitate the maintenance or replacement of the air filter membrane 6, a catheter five 7 is inserted at one end of the catheter four 41 away from the three-way B4. One end of the catheter five 7 is press-fitted and inserted inside the catheter four 41, and the air filter membrane 6 is installed at one end of the catheter five 7 away from the catheter four 41.

[0040] When it is necessary to maintain or replace the air filter membrane 6, pull the catheter five 7 in the direction away from the catheter four 41 until the catheter five 7 is disassembled and separated from the catheter four 41. At this time, the air filter membrane 6 at one end of the catheter five 7 can be maintained or cleaned, or a catheter five 7 with a new air filter membrane 6 can be installed on the catheter four 41.

[0041] The above are all the preferred embodiments of this application. The protection scope of this application is not limited hereby. Therefore, all equivalent changes made according to the structure, shape, and principle of this application shall be covered within the protection scope of this application.

Claims

1. A sterile sampling and harvesting device for a reactor, characterized in that: The invention comprises a liquid outlet pipe (10) for connecting to a reactor (1), wherein one end of the liquid outlet pipe (10) away from the reactor (1) is connected to a tee A (2), the other two ends of the tee A (2) are respectively connected to a conduit 1 (20) and a conduit 2 (21), the end of the conduit 1 (20) away from the tee A (2) is connected to a harvesting quick connector (3), the end of the conduit 2 (21) away from the tee A (2) is connected to a tee B (4), the other two ends of the tee B (4) are respectively connected to a conduit 3 (40) and a conduit 4 (41), the end of the conduit 3 (40) away from the tee B (4) is connected to a sampling quick connector (5), and the end of the conduit 4 (41) away from the tee B (4) is connected to an air filter device; and also comprises a clamping tool.

2. The aseptic sampling and harvesting device for a reactor according to claim 1, characterized in that: The conduit 1 (20), conduit 2 (21), conduit 3 (40) and conduit 4 (41) are all hoses.

3. The aseptic sampling and harvesting device for a reactor according to claim 2, characterized in that: The clamping tool is a hemostat.

4. The aseptic sampling and harvesting device for a reactor according to claim 1, characterized in that: The harvesting quick connector (3) is a harvesting quick connector (3) made of metal, and the sampling quick connector (5) is a sampling quick connector (5) made of metal.

5. The aseptic sampling and harvesting device for a reactor according to claim 1, characterized in that: The air filtering device is an air filter membrane (6).

6. The aseptic sampling and harvesting device for a reactor according to claim 1, characterized in that: Among the catheter 1 (20), the catheter 2 (21), the catheter 3 (40) and the catheter 4 (41), the catheter 2 (21) is the shortest.

7. The aseptic sampling and harvesting device for a reactor according to claim 1, characterized in that: The conduit four (41) is detachably connected to the air filter device.

8. The aseptic sampling and harvesting device for a reactor according to claim 7, characterized in that: The end of the conduit four (41) away from the tee B (4) is tightly plugged with the conduit five, and the end of the conduit five away from the conduit four (41) is fixedly connected with the air filter device.

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