Cell induction culture bag

Abstract

The utility model discloses a cell induction culture bag, which relates to cell therapy reagent consumables, and comprises a culture bag body, one side of the culture bag body is fixedly connected with a main pipeline, the main pipeline is connected with a pipeline assembly, the main pipeline is provided with a flow limiting assembly, the flow limiting assembly movably abuts against the main pipeline, and the main pipeline is connected with the pipeline assembly. The flow limiting assembly comprises a base on the outer surface of the main pipeline, and the inner ring of the base abuts against the outer pipe wall of the main pipeline in a matched mode. By rotating the fixing hole, the first connecting rod slides in the sliding groove so as to be closed, the area of the sliding piece can be increased, the main pipeline is clamped in all aspects to conduct liquid flow limiting, and the stress point clamped by the main pipeline and the stress area are relatively equal. The situation that in the long-time liquid stopping and flow limiting process, due to uneven stress, the outer surface of the main pipeline is damaged, liquid leakage occurs or other bacteria enter the main pipeline is avoided, it is guaranteed to a certain degree that final data are stably displayed, and the liquid stopping and flow limiting device is more stable during working.

Description

Technical Field

[0001] The utility model relates to the technical field of cell therapy reagent consumables, in particular to a cell induction culture bag. Background Art

[0002] Cell induction culture bag refers to a method of culturing cells in vitro, simulating the in vivo environment in vitro, and controlling the variables in the cell living environment, such as temperature, pH, nutritional conditions, etc., to be the same or similar to our in vivo environment, so that they can grow, reproduce and maintain their main structure and function.

[0003] For example, an existing patent (publication number: CN214881585U) discloses a cell induction culture bag and an NK cell induction culture bag, which are provided with a flow-stop clamp to prevent a large amount of liquid from flowing in or out, but cannot well control the amount of liquid flowing in or out.

[0004] However, the cell induction culture bag and NK cell induction culture bag designed above still have some shortcomings in actual use: although the flow-stop clamp on the pipeline of the culture bag can prevent a large amount of liquid inflow and outflow, the flow-stop clamp only contacts the pipeline at two points. The two clamping points will cause uneven force around the pipeline. During the long-term flow-stop clamping process, the pipeline may be damaged due to uneven force, which will eventually affect the presentation of the final data during cell culture or liquid inflow and outflow.

[0005] To this end, we designed a cell induction culture bag to solve the above problems. Utility Model Content

[0006] The purpose of the present invention is to provide a cell induction culture bag to solve the problems raised in the above background technology.

[0007] In order to solve the above technical problems, the utility model provides a cell induction culture bag, including a culture bag body, a main line fixedly connected to one side of the culture bag body, a pipeline assembly connected to the main line, a flow limiting assembly arranged on the main line, the flow limiting assembly and the main line movably abutting against each other, the flow limiting assembly including a base on the outer surface of the main line, the inner ring of the base cooperates and abuts against the outer pipe wall of the main line, a plurality of overlapping slides are movably arranged on the base, a plurality of the slides are provided with positioning holes, a first connecting rod is inserted into the bottom of the plurality of the slides, the first connecting rod is slidably inserted into the base, a second connecting rod is provided in the positioning hole, a fixing piece is also provided on the second connecting rod, and the slide is movably arranged between the base and the fixing piece.

[0008] Furthermore, the fixing member includes a base top seat connected to the second connecting rod, and a plurality of fixing holes are opened on the base top seat, and the second connecting rod is inserted into the fixing holes. The setting of the fixing holes can better connect the base base and the base top seat.

[0009] Furthermore, a sliding groove is provided on the base seat, the first connecting rod is slidably inserted in the sliding groove, and the slide is rotatably set between the base seat and the base top seat. The setting of the sliding groove can enable the first connecting rod to better open and close for clamping, and prevent motion interference problems.

[0010] Furthermore, two limiting members are symmetrically and movably arranged on both sides of the flow limiting component, and the two limiting members are against the outer pipe wall of the main pipeline, and the opposite side of the limiting member is against the flow limiting component. The setting of the limiting member can prevent the flow limiting component from being displaced when the liquid is flowing in and out after clamping.

[0011] Furthermore, a sub-velcro is fixedly provided on the inner surface of the limiting member, and a mother Velcro is provided on the opposite side of the sub-velcro. The sub-velcro and the mother Velcro fit together, and the setting of the sub-velcro and the mother Velcro can better set the limiting member on the main pipe.

[0012] Furthermore, the pipeline assembly includes a first branch pipeline and a second branch pipeline connected to the main pipeline, one end of the first branch pipeline is fixedly provided with a female Luer connector, and one end of the second branch pipeline is fixedly connected to a male Luer connector.

[0013] Furthermore, a two-way pipeline is fixedly connected to one side of the main pipeline, and the arrangement of the two-way pipeline can be used to carry out a small amount of liquid inflow and outflow into the culture bag body.

[0014] Furthermore, a label column is fixedly provided on one side of the culture bag body, and the setting of the label column can easily distinguish the culture bags.

[0015] Compared with the prior art, the beneficial effect of the present invention is that by rotating the fixing hole, the first connecting rod slides in the slide groove to close and increase the area closed by the slide and fully clamp the main line to limit the flow of liquid, which helps to make the force points and force areas clamped by the main line relatively equal, and prevents uneven force from occurring during the long-term liquid-stopping and flow-limiting process, causing the outer surface of the main line to be damaged, resulting in leakage or the entry of other bacteria, and to a certain extent ensures the stable presentation of the final data, making it more stable during operation.

[0016] Compared with the prior art, the beneficial effect of the present invention is that by symmetrically wrapping two limiting parts on both sides of the current limiting component, the sub-velcro and the main Velcro on the limiting parts are fit together, and the current limiting component is limited to one position, preventing the current limiting component from being displaced during the subsequent liquid inlet and outlet process, affecting the liquid inlet and outlet work. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall external structure of the utility model;

[0018] Figure 2 This is an exploded view of the current limiting component of the utility model;

[0019] Figure 3 This is a schematic diagram of the overall right side structure of the utility model;

[0020] Figure 4 This is an expanded view of the limiter of the utility model.

[0021] In the figure: 1. Culture bag body; 2. Main pipeline; 3. Current limiting assembly; 4. Base; 5. Slide; 6. Positioning hole; 7. First connecting rod; 8. Second connecting rod; 9. Base top seat; 10. Fixing hole; 11. Slide groove; 12. Limiting piece; 13. Male Velcro; 14. Female Velcro; 15. First branch pipeline; 16. Second branch pipeline; 17. Female Luer connector; 18. Male Luer connector; 19. Two-way pipeline; 20. Label column. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] See also Figure 1-4The utility model provides a technical solution: a cell induction culture bag, including a culture bag body 1, a main line 2 is fixedly connected to one side of the culture bag body 1, a pipeline assembly is connected to the main line 2, a flow limiting assembly 3 is arranged on the main line 2, and the flow limiting assembly 3 is movably abutted against the main line 2, and the flow limiting assembly 3 includes a base 4 on the outer surface of the main line 2, the inner ring of the base 4 cooperates with the outer pipe wall of the main line 2 to abut, and a plurality of overlapping slides 5 are movably provided on the base 4, and positioning holes 6 are opened on the plurality of slides 5, and a first connecting rod 7 is inserted into the bottom of the plurality of slides 5, which is slidably inserted in the base 4, and a second connecting rod 8 is arranged in the positioning hole 6. A fixing piece is also provided on the second connecting rod 8, and the slide 5 is movably arranged between the base 4 and the fixing piece.

[0024] The fixing member includes a base seat 9 connected to the second connecting rod 8 . The base seat 9 is provided with a plurality of fixing holes 10 , and the second connecting rod 8 is inserted into the fixing holes 10 .

[0025] A sliding groove 11 is further provided on the base seat 4 , the first connecting rod 7 is slidably inserted into the sliding groove 11 , and the slide 5 is rotatably arranged between the base seat 4 and the base top seat 9 .

[0026] The pipeline assembly includes a first branch pipeline 15 and a second branch pipeline 16 connected to the main pipeline 2. One end of the first branch pipeline 15 is fixedly provided with a female Luer connector 17, and one end of the second branch pipeline 16 is fixedly connected to a male Luer connector 18.

[0027] During specific implementation, the male Luer connector 18 is inserted into the cell induction culture bag equipped with the female Luer connector 17, and then the fixing hole 10 is rotated to make the first connecting rod 7 slide in the slide groove 11 to close and increase the closing area of the slide 5 and clamp the main line 2 in all aspects, which helps to make the force points and force areas clamped by the main line 2 relatively equal, and prevent uneven force from occurring during the long-term liquid-stopping and flow-limiting process, causing the outer surface of the main line 2 to be damaged, leaking, or other bacteria to enter. To a certain extent, it ensures the stable presentation of the final data, making it more stable during operation.

[0028] See Figure 1-4 As shown, two limiting members 12 are symmetrically and movably provided on both sides of the flow limiting component 3. The two limiting members 12 abut against the outer pipe wall of the main pipe 2, and the opposite side of the limiting member 12 abuts against the flow limiting component 3.

[0029] A sub-velcro 13 is fixedly provided on the inner surface of the limiting member 12 , and a main-velcro 14 is provided on the opposite side of the sub-velcro 13 , and the sub-velcro 13 and the main-velcro 14 fit together.

[0030] During specific implementation, two limiting parts 12 are symmetrically wrapped around the two sides of the current limiting component 3, so that the sub-Velcro 13 and the mother Velcro 14 on the limiting part 12 fit together, limiting the current limiting component 3 in one position to prevent the current limiting component 3 from being displaced during the subsequent liquid inlet and outlet process, affecting the liquid inlet and outlet work.

[0031] See Figure 1-4 A two-way pipe 19 is fixedly connected to one side of the main pipe 2. The setting of the two-way pipe 19 can be used to add and remove a small amount of liquid from the culture bag body 1.

[0032] See Figure 1-4 A label column 20 is fixedly provided on one side of the culture bag body 1 , and the setting of the two-way pipe 19 can be used to add and remove a small amount of liquid from the culture bag body 1 .

[0033] In addition, it should be noted that the two-way pipeline 19 has an internal rotating sealing interface and is equipped with a corresponding sealing joint to facilitate repeated puncture of a small amount of sampling and fluid replenishment by the syringe to prevent liquid leakage.

[0034] Working principle: When liquid supplement is needed, insert the male Luer connector 18 into the cell induction culture bag equipped with the female Luer connector 17, and symmetrically connect the two limiting parts 12 on both sides of the flow limiting component 3, so that the sub-velcro 13 and the female Velcro 14 on the limiting part 12 fit together, and limit the flow limiting component 3 to one position to prevent the displacement of the flow limiting component 3 during the subsequent liquid inlet and outlet process, which affects the liquid inlet and outlet work. Then rotate the fixing hole 10 to make the first connecting rod 7 slide in the slide groove 11 to close, increase the closed area of the slide 5 and clamp it in all directions. Holding the main line 2 helps to make the force points and force areas clamped by the main line 2 relatively equal, preventing uneven force from occurring during the long-term liquid-stopping and flow-limiting process, which may cause damage to the outer surface of the main line 2, leakage, or the entry of other bacteria. To a certain extent, it ensures the stable presentation of the final data, making it more stable during operation. Finally, the date of liquid inflow is marked on the label column 20 to facilitate the record of subsequent liquid replenishment and liquid discharge. If a small amount of solution sampling and replenishment is required, a syringe can be used to insert the syringe into the two-way pipeline 19 for small amounts of sampling and liquid replenishment.

Claims

1. A cell induction culture bag, comprising a culture bag body (1), one side of the culture bag body (1) is fixedly connected to a main line (2), and the main line (2) is connected to a pipeline assembly, characterized in that: A flow limiting component (3) is provided on the main pipe (2), and the flow limiting component (3) is movably opposed to the main pipe (2). The flow limiting component (3) includes a base (4) on the outer surface of the main pipe (2), and the inner ring of the base (4) is matched with the outer pipe wall of the main pipe (2) to be opposed. A plurality of overlapping slides (5) are also movably provided on the base (4), and a positioning hole (6) is provided on the plurality of slides (5). The bottoms of the plurality of slides (5) are all plugged with a first connecting rod (7), and the first connecting rod (7) is slidably inserted into the base (4). A second connecting rod (8) is provided in the positioning hole (6), and a fixing piece is also provided on the second connecting rod (8). The slide (5) is movably provided between the base (4) and the fixing piece.

2. A cell induction culture bag according to claim 1, characterized in that: The fixing member comprises a base seat (9) connected to the second connecting rod (8), a plurality of fixing holes (10) are provided on the base seat (9), and the second connecting rod (8) is inserted into the fixing holes (10).

3. A cell induction culture bag according to claim 2, characterized in that: The base seat (4) is also provided with a slide groove (11), the first connecting rod (7) is slidably inserted into the slide groove (11), and the slide plate (5) is rotatably arranged between the base seat (4) and the base top seat (9).

4. The cell induction culture bag according to claim 1, wherein: Two limiting members (12) are symmetrically and movably provided on both sides of the flow limiting component (3); the two limiting members (12) abut against the outer pipe wall of the main pipe (2); and the opposite side of the limiting member (12) abuts against the flow limiting component (3).

5. The cell induction culture bag according to claim 4, characterized in that: A sub-velcro (13) is fixedly provided on the inner surface of the limiting member (12), and a mother-velcro (14) is provided on the opposite side of the sub-velcro (13), and the sub-velcro (13) and the mother-velcro (14) are fitted together.

6. The cell induction culture bag according to claim 1, wherein: The pipeline assembly comprises a first branch pipeline (15) and a second branch pipeline (16) which are arranged in communication with the main pipeline (2); one end of the first branch pipeline (15) is fixedly provided with a female Luer connector (17); and one end of the second branch pipeline (16) is fixedly connected with a male Luer connector (18).

7. The cell induction culture bag according to claim 1, wherein: A two-way pipeline (19) is also fixedly connected to one side of the main pipeline (2).

8. The cell induction culture bag according to claim 1, wherein: A label column (20) is fixedly provided on one side of the culture bag body (1).

Images