Special programmed cooling box for cell culture cryopreservation bag
By designing a program cooling box for vertical boxes, doors and partitions, and using isopropanol to surround the frozen storage bag, the problems of low freezing efficiency and insufficient survival rate of large-volume cells are solved, and efficient and low-cost cell freezing is achieved.
Patent Information
- Application Number
- CN202422494576.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-16
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-10-16
AI Technical Summary
The existing program cooling box is not suitable for frozen storage of large-volume cell samples, resulting in low freezing efficiency and high cost, and insufficient cell survival rate.
A program cooling box including a vertical box, a box door, a partition and a liquid adding port was designed. The frozen storage bag was placed into multiple partitions through the partition, and the frozen storage bag was surrounded by isopropanol to achieve gradient cooling.
It improves the freezing efficiency and cell survival rate of large-volume cell samples, and reduces costs.
Smart Images
Figure CN223195413U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell freezing, in particular to a special program cooling box for cell culture freezing bags. Background Art
[0002] In the biopharmaceutical industry, prepared biological products, especially cells, need to be stored at -196°C to maintain their biological activity. This allows them to be removed from the -196°C cryogenic environment and used to prepare cells for culture when needed. Therefore, the quality of cryopreservation directly determines the biological activity and characteristics of cryopreserved cells.
[0003] To prevent ice crystals from damaging cells during cryopreservation, cells must be cooled slowly during cryopreservation. Currently, programmed cooling is typically performed using a programmed cooling instrument or a programmed cooling box. However, programmed cooling instruments are expensive and unsuitable for small-scale production and preparation, so programmed cooling boxes are often used.
[0004] Currently known programmed cooling boxes are all designed for cryopreservation tubes of low-volume cell freezing solutions such as 2ml and 5ml. The volume of frozen samples is small, and large-volume cell samples need to be packaged in large quantities, which is labor-intensive and time-consuming. In addition, a large number of programmed cooling boxes are required, which is not conducive to cost control. Utility Model Content
[0005] The technical problem to be solved by the utility model is to provide a program cooling box specially used for cell culture freezing bags, so as to solve the problems raised in the background technology.
[0006] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows.
[0007] A special cooling box for cell culture cryopreservation bags comprises a vertical box with a side opening, wherein a box door for sealing the vertical box is provided on the open side of the vertical box, wherein the vertical box comprises an inner box wall forming the side opening and an outer box wall provided on the outer side of the inner box wall, and a vertical box cavity is formed between the outer box wall and the inner box wall; the side opening of the vertical box is divided into a plurality of compartments for placing cryopreservation bags by a plurality of horizontally arranged partitions spaced from top to bottom, and a partition cavity communicating with the vertical box cavity is provided inside the partition; the box door comprises an inner door wall and a partition provided on the side of the vertical box to fit with the vertical box after the box door is closed. An outer door wall is provided on the outside of the inner door wall, and a door cavity is formed between the outer door wall and the inner door wall; a plurality of plug-in protrusions are provided on the inner side of the door, which are arranged one by one opposite to each partition and are used to be plugged into the outer ends of each partition to seal each partition after the door is closed, and a plug-in cavity connected to the door cavity is provided inside the plug-in protrusions; a vertical box liquid filling port is provided on the top of the vertical box, which is connected to the vertical box cavity and is used to add isopropyl alcohol to the vertical box cavity and the partition cavity, and a door liquid filling port is provided on the top of the door, which is connected to the door cavity and is used to add isopropyl alcohol to the door cavity and the plug-in cavity.
[0008] Preferably, the bottom upper surface of the inner box wall and the upper surface of each partition are respectively provided with anti-slip strips for avoiding the plug-in protrusions and for directly contacting the freezing bags to prevent the freezing bags from slipping.
[0009] Preferably, one side of the box door is pivotally connected to the upright box, and the other side of the box door is connected to the upright box via a provided lock assembly.
[0010] Preferably, the edge of the inner door wall is provided with a sealing strip for ensuring sealing between the box door and the upright box after the box door is closed.
[0011] Preferably, the vertical box liquid filling port and the box door liquid filling port are respectively provided with external threads, and the vertical box liquid filling port and the box door liquid filling port are respectively connected to a sealing cover with internal threads through the external threads.
[0012] Preferably, a handle is provided on the top of the upright box, and bottom corners are provided at the four corners of the bottom of the upright box.
[0013] Preferably, the tops of the outer box wall and the outer door wall are respectively provided with observation windows for conveniently observing the isopropyl alcohol liquid level.
[0014] Due to the adoption of the above technical solution, the technical progress achieved by the present invention is as follows.
[0015] The utility model can realize the cooling of cell culture cryopreservation bags by providing a vertical box, a box door, a partition, a plug-in protrusion, a vertical box liquid filling port and a box door liquid filling port, thereby improving the cryopreservation efficiency of large-volume cell samples, and can also realize that each cryopreservation bag is surrounded by isopropyl alcohol, so that the isopropyl alcohol is in close contact with the cells in the cryopreservation bag, and the survival rate of the cells during the cryopreservation process is significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a structural diagram of the utility model;
[0017] Figure 2 This is a schematic diagram of the internal structure of the upright box and the box door of the present utility model.
[0018] Among them: 1. Vertical box, 11. Outer box wall, 12. Inner box wall, 13. Vertical box cavity, 14. Vertical box filling port, 2. Box door, 21. Outer door wall, 22. Inner door wall, 23. Box door cavity, 25. Box door filling port, 3. Partition, 31. Partition cavity, 32. Anti-slip strip, 4. Interlayer, 5. Plug-in protrusion, 51. Plug-in cavity, 6. Lock assembly, 7. Sealing cover, 8. Handle, 9. Bottom corner, 10. Observation window. DETAILED DESCRIPTION
[0019] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation methods.
[0020] A special program cooling box for cell culture freezing bags, combined with Figures 1 to 2 As shown, it includes a vertical box 1 and a box door 2, wherein the vertical box 1 is a side opening; the box door 2 is arranged on the opening side of the vertical box 1, one side of the box door 2 is pivotally connected to the vertical box 1, and the other side of the box door 2 is connected to the vertical box 1 through a set lock assembly 6, and the box door 2 is used to seal the vertical box 1.
[0021] The vertical box 1 includes an inner box wall 12 and an outer box wall 11, wherein the inner box wall 12 forms a side opening; the outer box wall 11 is arranged on the outside of the inner box wall 12, and a vertical box cavity 13 is formed between the outer box wall 11 and the inner box wall 12. Several partitions 3 are arranged horizontally in the side opening of the vertical box 1 from top to bottom. The partitions 3 are divided into several compartments 4 by the partitions 3. The compartments 4 are used to place cryobags, which can accommodate large-volume cell samples. Anti-slip strips 32 are respectively provided on the bottom upper surface of the inner box wall 12 and the upper surface of each partition 3. The anti-slip strips 32 are arranged to avoid the insertion protrusions 5. The anti-slip strips 32 are used to directly contact the cryobags, thereby preventing the cryobags from slipping. A partition cavity 31 is also provided inside the partition 3, which is connected to the vertical box cavity 13.
[0022] The door 2 includes an inner door wall 22 and an outer door wall 21. The inner door wall 22 fits against the upright box 1 after the door 2 is closed, and a sealing strip is provided along the edge of the inner door wall 22 to ensure a tight seal between the door 2 and the upright box 1 after the door 2 is closed. The outer door wall 21 is provided on the outer side of the inner door wall 22, and a door cavity 23 is formed between the outer door wall 21 and the inner door wall 22. Several insertion protrusions 5 are provided on the inner side of the door 2, each of which is arranged opposite to each of the compartments 4. The insertion protrusions 5 are used to insert into the outer ends of the compartments 4 after the door 2 is closed, thereby sealing each compartment 4 from the outer end. An insertion cavity 51 is defined within each insertion protrusion 5, which communicates with the door cavity 23.
[0023] The top of the vertical box 1 is provided with a vertical box liquid filling port 14, which is in communication with the vertical box cavity 13. The vertical box liquid filling port 14 is used to add isopropyl alcohol to the vertical box cavity 13 and the partition cavity 31, so that the isopropyl alcohol surrounds the cryopreservation bag from the top, bottom, front, back and left side. The top of the door 2 is provided with a door liquid filling port 25, which is in communication with the door cavity 23. The door liquid filling port 25 is used to add isopropyl alcohol to the door cavity 23 and the plug-in cavity 51, so that the isopropyl alcohol surrounds the cryopreservation bag from the right side, thereby achieving that each cryopreservation bag is surrounded by isopropyl alcohol, so that the isopropyl alcohol is in close contact with the cells in the cryopreservation bag, and significantly improving the survival rate of the cells during the cryopreservation process.
[0024] External threads are respectively provided on the vertical box liquid filling port 14 and the box door liquid filling port 25, and the vertical box liquid filling port 14 and the box door liquid filling port 25 are respectively connected to a sealing cover 7 with internal threads through the external threads. After the addition of isopropyl alcohol to the vertical box 1 or the box door 2 is completed, the vertical box liquid filling port 14 or the box door liquid filling port 25 can be sealed by the sealing cover 7.
[0025] The tops of the outer box wall 11 and the outer door wall 21 are each provided with an observation window 10, through which the isopropyl alcohol liquid level can be easily observed. When the isopropyl alcohol needs to be replenished, the sealing cover 7 can be opened. When the isopropyl alcohol needs to be replaced, the sealing cover 7 can be opened, and the vertical box 1 or the door 2 can be turned over to pour out the isopropyl alcohol. After the isopropyl alcohol is poured out, it can be refilled. Alternatively, a liquid outlet can be provided at the bottom of the vertical box 1 and the door 2.
[0026] A handle 8 is provided on the top of the upright box 1 to facilitate carrying; bottom corners 9 are provided at the four corners of the bottom of the upright box 1 to play a supporting role and prevent the bottom of the upright box 1 from being worn.
[0027] When the utility model is used, isopropyl alcohol is injected into the vertical box 1 and the box door 2 through the vertical box liquid filling port 14 and the box door liquid filling port 25 respectively; then the freezing bags to be frozen are placed in each compartment 4, the box door 2 is fastened, and the program cooling box is carried out by the handle 8 and placed in a low temperature environment of -80°C. By utilizing the function of isopropyl alcohol itself to prevent the temperature from dropping rapidly, the temperature is gradually reduced to -80°C, and then the temperature is transferred to liquid nitrogen for storage.
Claims
1. A program cooling box for cell culture cryopreservation bags, comprising a side-opening vertical box (1), wherein the opening side of the vertical box (1) is provided with a box door (2) for sealing the vertical box (1), characterized in that: The vertical box (1) comprises an inner box wall (12) forming a side opening and an outer box wall (11) arranged on the outside of the inner box wall (12), and a vertical box cavity (13) is formed between the outer box wall (11) and the inner box wall (12); the side opening of the vertical box (1) is divided into a plurality of compartments (4) for placing frozen bags by a plurality of horizontally arranged partitions (3) arranged from top to bottom, and a partition cavity (31) is opened inside the partition (3) and communicated with the vertical box cavity (13); the box door (2) comprises an inner door wall (22) that fits with the vertical box (1) after the box door (2) is closed, and an outer door wall (21) arranged on the outside of the inner door wall (22), and a vertical box cavity (13) is formed between the outer door wall (21) and the inner door wall (22). The invention relates to a box door cavity (23); the inner side of the box door (2) is provided with a plurality of plug-in protrusions (5) which are arranged one by one opposite to each partition (4) and are used to be plugged into the outer ends of each partition (4) to block each partition (4) after the box door (2) is closed, and the plug-in protrusions (5) are provided with a plug-in cavity (51) which is connected to the box door cavity (23); the top of the vertical box (1) is provided with a vertical box liquid filling port (14) which is connected to the vertical box cavity (13) and is used to add isopropyl alcohol to the vertical box cavity (13) and the partition cavity (31); the top of the box door (2) is provided with a box door liquid filling port (25) which is connected to the box door cavity (23) and is used to add isopropyl alcohol to the box door cavity (23) and the plug-in cavity (51).
2. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: The bottom upper surface of the inner box wall (12) and the upper surface of each partition (3) are respectively provided with anti-slip strips (32) for avoiding the plug-in protrusions (5) and for directly contacting the frozen storage bag to prevent the frozen storage bag from slipping.
3. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: One side of the box door (2) is pivotally connected to the upright box (1), and the other side of the box door (2) is connected to the upright box (1) via a provided lock assembly (6).
4. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: The edge of the inner door wall (22) is provided with a sealing strip for ensuring sealing between the box door (2) and the upright box (1) after the box door (2) is closed.
5. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: The vertical box liquid filling port (14) and the box door liquid filling port (25) are respectively provided with external threads, and the vertical box liquid filling port (14) and the box door liquid filling port (25) are respectively connected to a sealing cover (7) with internal threads through the external threads.
6. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: The top of the upright box (1) is provided with a handle (8), and the four corners of the bottom of the upright box (1) are provided with bottom corners (9).
7. The cell culture cryopreservation bag dedicated program cooling box according to claim 1, characterized in that: The tops of the outer box wall (11) and the outer door wall (21) are respectively provided with observation windows (10) for conveniently observing the isopropyl alcohol liquid level.