Automatic identification device for measuring microorganisms in water by enzyme substrate method

Abstract

The utility model relates to the technical field of microorganism automatic identification, in particular to an automatic identification device for measuring microorganisms in water by an enzyme substrate method, which comprises a constant-temperature incubator, a controller, a discharging mechanism, a counting mechanism and a covering mechanism, the feeding mechanism comprises two storage areas and a plurality of culture dishes, the counting mechanism comprises a photographic microscope and a position adjusting assembly, and the covering mechanism comprises a plurality of sealing shells and a plurality of rotating assemblies. The automatic microbe counting device can perform automatic microbe counting work on a culture sample in a preset culture time, does not need manual operation of a laboratory technician, and avoids influence on accuracy of a determination result due to exponential increase of the microbe due to culture time prolonging caused by the fact that the laboratory technician is not on the spot.

Description

Technical Field

[0001] The utility model relates to the technical field of automatic identification of microorganisms, in particular to an automatic identification device for determining microorganisms in water using an enzyme substrate method. Background Art

[0002] Municipal water supply network monitoring requires regular testing and analysis of water samples from various inspection points, such as microbial analysis, to ensure water quality and water safety for residents. Enzyme substrate methods for determining the total microbial count in water rely on enzyme activity within the microbial colonies. Commonly used enzymes include alkaline phosphatase and acid phosphatase. These enzymes react with substrates to produce color or a color-developing substance, which can then be used to assess and determine the total number of colonies in a sample.

[0003] However, the current microbial determination in water samples has the following problems:

[0004] 1. The counting of microorganisms in water is divided into 24h and 48h. Water samples in different time periods need to be promptly removed from the culture dishes for counting. Otherwise, the extended time will cause the microorganisms to grow exponentially and affect other samples.

[0005] 2. Due to the large number of water samples and the different times required for technical testing, the experimenters may miss some and fail to count them in time, resulting in inaccurate experimental data. Utility Model Content

[0006] The purpose of the utility model is to provide an automatic identification device for determining microorganisms in water using an enzyme substrate method.

[0007] To achieve this purpose, the present invention adopts the following technical solutions:

[0008] Provided is an automatic identification device for determining microorganisms in water using an enzyme substrate method, comprising a constant temperature incubator, wherein a first partition is fixedly provided on the inner bottom of the constant temperature incubator;

[0009] It also includes a controller, a discharge mechanism, a counting mechanism and a covering mechanism;

[0010] The controller is fixed on the outer wall of the constant temperature incubator;

[0011] The material discharging mechanism is arranged on the top of the first partition, and the material discharging mechanism includes two storage areas and a plurality of culture dishes. The two storage areas are symmetrically arranged at the two ends of the top of the first partition, and the plurality of culture dishes are respectively arranged inside the two storage areas;

[0012] The counting mechanism is arranged on the top of the first partition, and the counting mechanism includes a photographic microscope and a position adjustment component, the position adjustment component is arranged between the two storage areas, and the photographic microscope is arranged on the position adjustment component;

[0013] The covering mechanism is arranged on the top of the first partition. The covering mechanism includes several sealed shells and several rotating components. Each rotating component is arranged next to a culture dish. Each sealed shell is fixed on a rotating component. The photographic microscope, the position adjustment component and each rotating component are electrically connected to the controller.

[0014] Preferably, each storage area includes a second partition and four storage cavities. The second partition is fixed to one end of the top of the first partition, and the four storage cavities are arranged on the side of the second partition through five third partitions.

[0015] Preferably, a support plate is fixedly provided inside each storage cavity, a hollow plate is provided on the top of each support plate, and each culture dish is located on the top of a hollow plate.

[0016] Preferably, the position adjustment component includes an electric slide, a cylinder, a lifting plate, a first motor and a rotating plate. The electric slide is fixed on the top of the first partition plate. A slider is slidably provided on the electric slide. The cylinder is fixed on the top of the slider through a connecting plate. The lifting plate is fixed on its output end. A guide rod is fixed on the top of the connecting plate. The lifting plate is slidably connected to the guide rod. The first motor is fixed on the top of the lifting plate. The rotating plate is fixed on its output end. The photographic microscope is fixed on the bottom end of the rotating plate away from the first motor. The cylinder and the first motor are both electrically connected to the controller.

[0017] Preferably, each rotating assembly includes a second motor, a first gear, a second gear and a rotating shaft. The second motor is fixed on the top of the first partition, the first gear is fixed on its output end, the rotating shaft is rotatably arranged on the top of the first partition, the second gear is fixed on the rotating shaft, the first gear and the second gear are meshed and connected, and the first gear is smaller than the second gear. The sealing shell is fixed on the rotating shaft through two connecting rods, and a sealing ring is fixed on the outer wall of each sealing shell. The second motor is electrically connected to the controller.

[0018] Preferably, a timing sensor is fixedly provided on the outer wall of the constant temperature incubator, and the timing sensor is electrically connected to the controller.

[0019] Preferably, a temperature control zone is provided between the bottom of the first partition and the inner bottom of the constant temperature incubator, and a plurality of flow slots are symmetrically provided at both ends of the first partition.

[0020] Preferably, a discharge port is provided on the top of the constant temperature incubator, a cover is hingedly provided inside the discharge port, a plurality of glass observation windows are provided on the top of the cover, each storage cavity is located below a glass observation window, and a handle is fixed on the top of the cover.

[0021] Beneficial effects of the utility model:

[0022] 1. The utility model is designed with a discharge mechanism, namely two storage areas and a plurality of culture dishes. Water samples to be tested in 24 hours and 48 hours are placed in the culture dishes in the two storage areas respectively. At the same time, by designing a timing sensor, the microorganisms can be automatically counted once the incubation time reaches 24 hours and 48 hours respectively. There is no need for the experimenter to constantly monitor the incubator and remove the culture dishes in time for counting, thus avoiding the incubation time being prolonged and the exponential growth of microorganisms affecting the measurement work.

[0023] 2. The utility model is designed with a counting mechanism, namely a photographic microscope and a position adjustment component, which can automatically take photos and count the water sample microorganisms in the four storage chambers in each storage area when the culture time reaches 24 hours and 48 hours. It will not cause omissions and failure to count in time due to the large number of water samples and the different times required for technical inspections, and will not cause confusion, thereby improving the detection effect.

[0024] 3. The utility model is designed with a covering mechanism, namely a plurality of sealing shells and a plurality of rotating components. When the number of microorganisms in the water sample in each storage chamber is photographed and counted, the rotating component is started by the controller, thereby driving the sealing shell to rotate toward the end close to the culture dish until the sealing ring on the sealing shell is tightly attached to the top of the culture dish, ensuring that the tested water samples can be separated from other untested water samples, preventing the large-scale growth of microorganisms from affecting the counting and measurement of other samples, improving the accuracy of counting, and further improving the accuracy of water sample measurement, without causing errors, and ensuring the safety of residents' water use.

[0025] 4. The utility model is designed with two storage areas, each of which includes a second partition and four storage cavities. The second partition is fixed at one end of the top of the first partition, and the four storage cavities are arranged on the side of the second partition through five third partitions. Each storage cavity is used to place a culture dish, the second partition is used to separate the storage cavity and the position adjustment component, and the five third partitions are used to separate four rows of water samples. The inside of each storage cavity can also be posted with a label of the pipe network point to which the water sample belongs, which is convenient for subsequent distinction and accurate identification of the water quality of the water sample at each pipe network point.

[0026] 5. The utility model is designed with several glass observation windows. Each storage cavity is located below a glass observation window. The glass observation windows can be used to observe the cultivation status of microorganisms in the water samples in the eight storage cavities in real time, which is convenient for timely adjustment when problems occur, thereby facilitating the smooth completion of the measurement work. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings in the embodiments of the present invention.

[0028] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0029] Figure 2 This is a schematic diagram of the three-dimensional structure of the utility model without the cover plate;

[0030] Figure 3 for Figure 2 A magnified view of point A in the figure;

[0031] Figure 4 This is a schematic cross-sectional view of the constant temperature incubator and two third partitions thereof of the present invention;

[0032] Figure 5 for Figure 4 Enlarged view of point B in FIG.

[0033] Figure 6 A three-dimensional exploded schematic diagram of one of the support plates, hollow plates and culture dishes of the present invention;

[0034] In the figure: constant temperature incubator 1, first partition 2, controller 3, storage area 4, culture dish 5, photographic microscope 6, position adjustment component 7, sealing shell 8, rotating component 9, second partition 10, storage chamber 11, third partition 12, support plate 13, hollow plate 14, electric slide 15, cylinder 16, lifting plate 17, first motor 18, rotating plate 19, slider 20, second motor 21, first gear 22, second gear 23, rotating shaft 24, sealing ring 25, timing sensor 26, temperature control area 27, circulation slot 28, cover plate 29, glass observation window 30. DETAILED DESCRIPTION

[0035] The technical solution of the present invention will be further described below with reference to the accompanying drawings and through specific implementation methods.

[0036] Among them, the drawings are only used for illustrative purposes and represent only schematic diagrams rather than actual pictures, and should not be understood as limitations on this patent; in order to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product.

[0037] Reference Figures 1 to 6 As shown, an automatic identification device for determining microorganisms in water using an enzyme substrate method comprises a constant temperature incubator 1, wherein a first partition plate 2 is fixedly provided on the inner bottom of the constant temperature incubator 1;

[0038] It also includes a controller 3, a discharge mechanism, a counting mechanism and a covering mechanism;

[0039] The controller 3 is fixed on the outer wall of the constant temperature incubator 1;

[0040] The discharge mechanism is arranged on the top of the first partition 2, and includes two storage areas 4 and a plurality of culture dishes 5. The two storage areas 4 are symmetrically arranged at both ends of the top of the first partition 2, and the plurality of culture dishes 5 are respectively arranged inside the two storage areas 4;

[0041] The counting mechanism is arranged on the top of the first partition 2, and the counting mechanism includes a photographic microscope 6 and a position adjustment component 7. The position adjustment component 7 is arranged between the two storage areas 4, and the photographic microscope 6 is arranged on the position adjustment component 7;

[0042] The covering mechanism is arranged on the top of the first partition 2. The covering mechanism includes several sealed shells 8 and several rotating components 9. Each rotating component 9 is arranged next to a culture dish 5. Each sealed shell 8 is fixed on a rotating component 9. The photographic microscope 6, the position adjustment component 7 and each rotating component 9 are electrically connected to the controller 3.

[0043] Reference Figures 1 to 6 As shown, each storage area 4 includes a second partition 10 and four storage chambers 11. The second partition 10 is fixed at one end of the top of the first partition 2. The four storage chambers 11 are arranged on the side of the second partition 10 through five third partitions 12. Each storage chamber 11 is used to place a culture dish 5. The second partition 10 is used to separate the storage chamber 11 and the position adjustment component 7. The five third partitions 12 are used to separate four rows of water samples. The inside of each storage chamber 11 can also be posted with a label of the pipe network point to which the water sample belongs, which is convenient for subsequent distinction and accurate identification of the water quality of the water sample at each pipe network point.

[0044] Reference Figures 1 to 6 As shown, a support plate 13 is fixedly provided inside each storage cavity 11, and a hollow plate 14 is provided on the top of each support plate 13. Each culture dish 5 is located on the top of a hollow plate 14. The support plate 13 is used to place the culture dish 5 containing the water sample, and the hollow plate 14 is used to support the bottom of the culture dish 5. At the same time, it is designed to be a hollow structure to facilitate air circulation in the constant temperature incubator 1 and maintain the cultivation of microorganisms in the water sample in the culture dish 5.

[0045] Reference Figures 1 to 6As shown, the position adjustment assembly 7 includes an electric slide 15, a cylinder 16, a lifting plate 17, a first motor 18 and a rotating plate 19. The electric slide 15 is fixed on the top of the first partition 2. A slider 20 is slidably provided on the electric slide 15. The cylinder 16 is fixed on the top of the slider 20 through a connecting plate. The lifting plate 17 is fixed on its output end. A guide rod is fixed on the top of the connecting plate. The lifting plate 17 is slidably connected to the guide rod. The first motor 18 is fixed on the top of the lifting plate 17. The rotating plate 19 is fixed on its output end. The photographic microscope 6 is fixed on the rotating plate 19 away from the first motor 1 At the bottom end of 8, the cylinder 16 and the first motor 18 are electrically connected to the controller 3. When the timing sensor 26 detects that the culture time reaches 24 hours, the reaction between the colonies in the microorganisms and the enzyme substrate is completed, and a signal is sent to the controller 3, thereby starting the cylinder 16 through the controller 3, and driving the lifting plate 17 and the photo microscope 6 thereon to rise above the top of the second partition 10 through the output end of the cylinder 16, and then starting the first motor 18 through the controller 3, thereby driving the rotating plate 19 on its output end to rotate toward the four storage chambers 11 near the top end of the first partition 2 until the bottom of the rotating plate 19 is reached. The photographic microscope 6 is rotated to the top of the first storage chamber 11, and the number of microorganisms in the water sample in the culture dish 5 in the first storage chamber 11 is photographed, and the physical image is transmitted to the internal storage of the controller 3 through digital-to-analog conversion. The controller 3 is internally designed with a counting module, which can automatically count the physical image of the microorganisms taken, so as to facilitate the calculation of the number of microorganisms generated in the water sample in 24 hours and judge the water quality. When the number of microorganisms in the first storage chamber 11 is counted, the electric slide 15 drives the slider 20 to slide horizontally, thereby driving the top of the slider 20 to move horizontally. The photographic microscope 6 slides horizontally to take photos and count the number of microorganisms in the water samples in the other three storage chambers 11. When the timing sensor 26 detects that the culture time of microorganisms in the water sample reaches 24 hours, it sends a signal to the controller 3, which starts the cylinder 16 and the first motor 18 through the controller 3 to rotate the photographic microscope 6 to the inside of the first storage chamber 11 in another row. According to the above steps, the number of microorganisms in the other row of 4 water samples that need to reach 48 hours is photographed and counted, and transmitted to the controller 3 for automatic counting, thereby completing the measurement of all water samples.

[0046] Reference Figures 1 to 6As shown, each rotating assembly 9 includes a second motor 21, a first gear 22, a second gear 23 and a rotating shaft 24. The second motor 21 is fixedly arranged on the top of the first partition 2, the first gear 22 is fixedly arranged on its output end, the rotating shaft 24 is rotatably arranged on the top of the first partition 2, the second gear 23 is fixedly arranged on the rotating shaft 24, the first gear 22 and the second gear 23 are meshed and connected, and the first gear 22 is smaller than the second gear 23. The sealed shell 8 is fixedly arranged on the rotating shaft 24 through two connecting rods, and a sealing ring 25 is fixedly arranged on the outer wall of each sealed shell 8. The second motor 21 is electrically connected to the controller 3. When the water sample in each storage chamber 11 is After the number of microorganisms is photographed and counted, the second motor 21 is started through the controller 3, so that its output end rotates. Since its output end is fixedly connected to the first gear 22, the second gear 23 is fixedly connected to the rotating shaft 24, and the sealing shell 8 is fixedly connected to the rotating shaft 24 through two connecting rods, the sealing shell 8 is driven to rotate toward the end close to the culture dish 5 until the sealing ring 25 on the sealing shell 8 is tightly attached to the top of the culture dish 5, ensuring that it can be separated from other untested samples, preventing the large-scale growth of microorganisms from affecting the counting and measurement of other samples, improving the accuracy of counting, and further improving the accuracy of water sample measurement, without causing errors, ensuring the safety of residents' water use.

[0047] Reference Figures 1 to 6 As shown, a timing sensor 26 is fixed on the outer wall of the constant temperature incubator 1, and the timing sensor 26 is electrically connected to the controller 3. The timing sensor 26 is set to count once every 24 hours and 48 hours, and cooperates with the controller 3. When the incubation time reaches 24 or 48 hours, it is convenient to take pictures and count the number of microorganisms in different water samples in the eight storage chambers 11 in time, without omissions, thereby improving the measurement effect.

[0048] Reference Figures 1 to 6 As shown, a temperature control zone 27 is provided between the bottom of the first partition 2 and the inner bottom of the constant temperature incubator 1, and a plurality of flow slots 28 are symmetrically provided at both ends of the first partition 2. A heater or refrigerator is fixedly installed in the temperature control zone 27, which is mainly used to heat or cool the air inside the constant temperature incubator to a temperature suitable for the reproduction and cultivation of microorganisms in water, and then transported to the interior of the eight storage chambers 11 through the plurality of flow slots 28 at both ends of the first partition 2, so as to facilitate the cultivation of microorganisms in the culture dish 5, thereby ensuring the smooth progress of subsequent microbial counting work.

[0049] Reference Figures 1 to 6As shown, the top of the constant temperature incubator 1 is provided with a discharge port, and a cover 29 is hingedly provided inside the discharge port. Several glass observation windows 30 are provided on the top of the cover 29. Each storage cavity 11 is located below a glass observation window 30. A handle is fixed on the top of the cover 29. When performing a measurement, the water samples taken are first placed in the eight culture dishes 5 in the eight storage cavities 11 respectively, and then the corresponding enzyme substrate is added to each culture dish 5 and mixed evenly with the water sample. Four of the storage cavities 11 arranged in a row are used to place water samples that need to be measured for microorganisms for 24 hours, and the other four storage cavities 11 arranged in a row are used to place water samples that need to be measured for microorganisms for 48 hours. This facilitates subsequent counting work and does not cause confusion. After the water samples are placed, the cover 29 is manually closed, and several glass observation windows 30 can be used to observe the culture status of microorganisms in the water samples in the eight storage cavities 11 in real time, which is convenient for timely adjustment when problems arise.

Claims

1. An automatic identification device for determining microorganisms in water using an enzyme substrate method, comprising a constant temperature incubator (1), a first partition (2) fixedly provided on the inner bottom of the constant temperature incubator (1), and characterized in that: It also includes a controller (3), a discharge mechanism, a counting mechanism and a covering mechanism; The controller (3) is fixedly mounted on the outer wall of the constant temperature incubator (1); The discharge mechanism is arranged on the top of the first partition (2), and comprises two storage areas (4) and a plurality of culture dishes (5). The two storage areas (4) are symmetrically arranged at both ends of the top of the first partition (2), and the plurality of culture dishes (5) are respectively arranged inside the two storage areas (4); The counting mechanism is arranged on the top of the first partition (2), and the counting mechanism includes a photographic microscope (6) and a position adjustment component (7). The position adjustment component (7) is arranged between the two storage areas (4), and the photographic microscope (6) is arranged on the position adjustment component (7); The covering mechanism is arranged on the top of the first partition (2), and the covering mechanism includes a plurality of sealing shells (8) and a plurality of rotating components (9). Each rotating component (9) is arranged beside a culture dish (5), and each sealing shell (8) is fixed on a rotating component (9). The photographic microscope (6), the position adjustment component (7), and each rotating component (9) are electrically connected to the controller (3).

2. The automatic identification device for determining microorganisms in water by an enzyme substrate method according to claim 1, characterized in that: Each storage area (4) includes a second partition (10) and four storage cavities (11). The second partition (10) is fixedly arranged at one end of the top of the first partition (2). The four storage cavities (11) are arranged on the side of the second partition (10) through five third partitions (12).

3. The automatic identification device for determining microorganisms in water by an enzyme substrate method according to claim 2, characterized in that: A support plate (13) is fixedly provided inside each storage cavity (11), a hollow plate (14) is provided on the top of each support plate (13), and each culture dish (5) is located on the top of a hollow plate (14).

4. The automatic identification device for determining microorganisms in water by an enzyme substrate method according to claim 3, characterized in that: The position adjustment component (7) includes an electric slide (15), a cylinder (16), a lifting plate (17), a first motor (18) and a rotating plate (19), wherein the electric slide (15) is fixed on the top of the first partition (2), a slider (20) is slidably provided on the electric slide (15), the cylinder (16) is fixed on the top of the slider (20) through a connecting plate, the lifting plate (17) is fixed on the output end thereof, a guide rod is fixed on the top of the connecting plate, the lifting plate (17) is slidably connected to the guide rod, the first motor (18) is fixed on the top of the lifting plate (17), the rotating plate (19) is fixed on the output end thereof, the photographic microscope (6) is fixed on the bottom end of the rotating plate (19) away from the first motor (18), and the cylinder (16) and the first motor (18) are both electrically connected to the controller (3).

5. The automatic identification device for determining microorganisms in water by an enzyme substrate method according to claim 4, characterized in that: Each rotating assembly (9) includes a second motor (21), a first gear (22), a second gear (23) and a rotating shaft (24). The second motor (21) is fixedly arranged on the top of the first partition (2), the first gear (22) is fixedly arranged on the output end thereof, the rotating shaft (24) is rotatably arranged on the top of the first partition (2), the second gear (23) is fixedly arranged on the rotating shaft (24), the first gear (22) and the second gear (23) are meshed and connected, and the first gear (22) is smaller than the second gear (23), the sealing shell (8) is fixedly arranged on the rotating shaft (24) through two connecting rods, and a sealing ring (25) is fixedly arranged on the outer wall of each sealing shell (8). The second motor (21) is electrically connected to the controller (3).

6. The automatic identification device for determining microorganisms in water by enzyme substrate method according to claim 5, characterized in that: A timing sensor (26) is fixedly provided on the outer wall of the constant temperature incubator (1), and the timing sensor (26) is electrically connected to the controller (3).

7. The automatic identification device for determining microorganisms in water by enzyme substrate method according to claim 6, characterized in that: A temperature control area (27) is provided between the bottom of the first partition (2) and the inner bottom of the constant temperature incubator (1), and a plurality of flow slots (28) are symmetrically provided at both ends of the first partition (2).

8. The automatic identification device for determining microorganisms in water by enzyme substrate method according to claim 7, characterized in that: A discharge port is provided at the top of the constant temperature incubator (1), a cover plate (29) is hingedly provided inside the discharge port, a plurality of glass observation windows (30) are provided at the top of the cover plate (29), each storage cavity (11) is located below a glass observation window (30), and a handle is fixedly provided at the top of the cover plate (29).

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