Fixture for biological tissue transfer

Abstract

The embodiment of the utility model provides a clamp for transferring biological tissues, which comprises a first clamping part, a second clamping part and a third clamping part, the first clamping part is provided with at least one carrying rod clamping groove, a carrying rod is clamped in the carrying rod clamping groove, and the end part of the carrying rod is loaded with the biological tissues; the second clamping part is provided with at least one sleeve clamping groove, and a sleeve is clamped in the sleeve clamping groove; wherein the end part of the carrying rod is opposite to the end part of the sleeve, and the first clamping part moves along the axial direction, so that the biological tissues contained in the carrying rod are transferred into the sleeve. The clamp device is simple in operation and high in success rate when being used for transferring biological tissues such as embryos.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, in particular to a clamp used for transferring biological tissues. Background Art

[0002] Despite the remarkable success of cryopreservation technology, particularly vitrification, in human medicine and its widespread application in reproductive medicine, its application in the livestock industry has been significantly limited. This limitation is primarily due to factors such as cost, expertise, and pasture environment. First, vitrification requires expensive equipment and expertise, which may be impractical in the livestock industry. Specifically, thawed embryos need to be transferred to recipient cows as quickly as possible. Embryologists use a mouth pipette under a microscope to transfer embryos from the carrier straw to straws, a process that requires meticulous training and a specialized laboratory environment. In pasture environments, there is often a lack of personnel with these specialized skills, making the application of this technology difficult. Furthermore, pastures are often located in remote areas and lack the necessary infrastructure and equipment, such as microscopes and freezing equipment, making the application of the technology even more difficult.

[0003] Prior art embryo transfer required a skilled embryologist and required prompt transfer to the recipient cow after thawing. However, poor pasture conditions reduced the success rate. Furthermore, manual embryo transfer and straw loading were cumbersome, inefficient, and prone to errors. Utility Model Content

[0004] The main purpose of the utility model is to provide a clamp for biological tissue transfer, so as to solve the problems of complicated operation and low success rate of artificial embryo transfer technology in the prior art.

[0005] According to an embodiment of the present invention, a clamp for transferring biological tissue is proposed, which includes: a first clamping part, the first clamping part having at least one carrier rod clamping groove, in which a carrier rod is clamped, and the end of the carrier rod contains biological tissue; a second clamping part, the second clamping part having at least one sleeve clamping groove, in which a sleeve is clamped; wherein the end of the carrier rod and the end of the sleeve are opposite to each other, and the first clamping part moves axially to transfer the biological tissue contained in the carrier rod to the sleeve.

[0006] Wherein, the clamp further includes: a docking portion, which is detachably arranged between the first clamping portion and the second clamping portion, and has at least one positioning through hole, and the carrier rod passes through the positioning through hole so that the end of the carrier rod and the end of the sleeve are opposite to each other.

[0007] The positioning through hole includes a rod positioning hole opened toward the first clamping portion and a sleeve positioning hole opened toward the second clamping portion. The rod positioning hole and the sleeve positioning hole are connected, and the rod positioning hole and the sleeve positioning hole are trumpet-shaped and gradually expand from the inside to the outside.

[0008] Wherein, an opening is provided above the positioning hole of the carrier rod.

[0009] Wherein, the clamp further comprises: one or more rod support frames, each of the rod support frames carrying a plurality of rods.

[0010] Wherein, the first clamping portion further includes: a locking structure for locking the carrier rod in the carrier rod clamping groove.

[0011] Wherein, the clamp further includes: a slider arranged below the first clamping part, and the slider drives the first clamping part to perform axial movement.

[0012] Wherein, the sleeve clamping groove and the carrier rod clamping groove have the same height.

[0013] Wherein, the carrier rod is provided with a groove near the front end, and the groove contains liquid containing at least biological tissue.

[0014] Wherein, the biological tissue includes an embryo or an egg.

[0015] According to the technical solution of the present invention, a first clamping portion for clamping a carrier rod carrying biological tissue and a second clamping portion for clamping a sleeve are provided, and the end of the carrier rod and the end of the sleeve are opposite to each other. When the first clamping portion moves axially relative to the second clamping portion, the carrier rod and the sleeve can be docked and the biological tissue in the carrier rod can be transferred into the sleeve. The transfer of biological tissues such as embryos using this clamp device is simple and has a high success rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0017] Figure 1 is a schematic diagram of a clamp according to an embodiment of the present utility model;

[0018] Figure 2 is a schematic diagram of a first clamping portion according to an embodiment of the present utility model;

[0019] Figure 3 1 is a schematic diagram of a carrier rod according to an embodiment of the present utility model;

[0020] Figure 4 is a schematic diagram of a clamp according to another embodiment of the present utility model;

[0021] Figure 5A and 5B is a schematic diagram of a docking portion according to an embodiment of the present utility model;

[0022] Figure 6 is a schematic diagram of a clamp according to yet another embodiment of the present utility model;

[0023] Figure 7 Schematic diagram of a rod support frame carrying rods according to an embodiment of the present invention. DETAILED DESCRIPTION

[0024] To make the purpose, technical solutions, and advantages of the present invention more clear, the following will provide a clear and complete description of the technical solutions of the present invention in conjunction with specific embodiments of the present invention and the corresponding drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0025] The technical solutions provided by various embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0026] refer to Figure 1 According to an embodiment of the present invention, a clamp 1 for transferring biological tissue includes a clamp body 10, a first clamping portion 11, and a second clamping portion 12. The first clamping portion 11 and the second clamping portion 12 are disposed opposite each other on the clamp body 10, wherein the first clamping portion 11 is used to clamp and secure the carrier rod 20, and the second clamping portion 12 is used to clamp and secure the cannula 30.

[0027] Combined with reference Figure 1 and Figure 2 The first clamping portion 11 is installed near one end of the clamp body 10. The first clamping portion 11 is used to clamp the carrier rod 20 and can drive the carrier rod 20 to move axially relative to the clamp body 10. Specifically, the first clamping portion 11 is defined by one or more clamping grooves 111, which can also be called carrier rod clamping grooves. The carrier rod 20 can be clamped in the clamping groove 111 and locked in the clamping groove 111 by a locking structure 112, wherein the locking structure 112 can be a pin. Continuing to refer to the drawings, a slider 113 is fixedly connected to the bottom of the first clamping portion 11. The slider 113 can slide axially on the clamp body 10, so that the slider 113 can drive the first clamping portion 11 and the carrier rod 20 clamped therein to perform axial reciprocating motion on the clamp body 10.

[0028] refer to Figure 3 The carrier rod 20 is generally elongated and includes a handle 21, a carrier body 22, and a front sheet 23. The handle 21 is where the operator's hand directly contacts the carrier rod. The front sheet 23 has at least one groove 231, and for example, two or more grooves may be provided. The groove 231 is used to hold biological materials or tissues such as embryos, eggs, or cells, as well as solutions that protect these materials or tissues. When the carrier rod 20 is secured, the portion near the handle 21 is locked within the clamping groove 111.

[0029] Continue to refer Figure 1 At the other end of the clamp body 10, a second clamping portion 12 is provided for clamping and securing the cannula 30. The second clamping portion 12 includes one or more clamping grooves, which can also be referred to as cannula clamping grooves. The height of the clamping grooves of the second clamping portion 12 is the same as the height of the carrier straw clamping grooves of the first clamping portion 11. This allows the cannula 30 and carrier straw 20 to be at the same horizontal position with their ends facing each other, facilitating docking or contact between the cannula 30 and the carrier straw 20. The cannula 30 has a volume of 0.25-0.5 mL and can be a straw, centrifuge tube, or test tube. It is made of biocompatible materials and can be used for cryopreserving egg and embryo samples, as well as transferring embryos or eggs into target animals. In this embodiment, the second clamping portion 12 is fixedly connected to the clamp body 10, or the second clamping portion 12 is integrally formed with the clamp body 10. When the carrier rod 20 (clamped by the first clamping portion 11) moves axially relative to the clamp body 10, the sleeve 30 (clamped by the second clamping portion 12) remains stationary relative to the clamp body 10. It should be noted that the aforementioned axial movement may be reciprocating, shaking, rocking, or motion, and that the carrier rod 20 and sleeve 30 maintain contact during such movement.

[0030] Combined with reference Figure 4 、 Figure 5A and Figure 5BIn some embodiments of the present invention, to facilitate docking and accurate alignment of the carrier rod 20 and the sleeve 30, a docking portion 13 may be provided between the first clamping portion 11 and the second clamping portion 12. The docking portion 13 is a removable component for easy removal, cleaning, and disinfection. It includes a trumpet-shaped opening that defines the docking position of the carrier rod 20 and the sleeve 30. The carrier rod 20 and the sleeve 30 approach the docking portion 13 from either side. The trumpet-shaped opening of the docking portion 13 is open at both ends and expands from the inside outward. It receives and guides the carrier rod 20 and the sleeve 30, facilitating alignment of the ends of the two rods 20 and sleeve 30. Specifically, at least one carrier rod positioning hole 131 and at least one sleeve positioning hole 132 are defined on either side of the docking portion 13. The carrier rod positioning hole 131 faces the first clamping portion 11, while the sleeve positioning hole 132 faces the second clamping portion 12. Both the carrier rod positioning hole 131 and the sleeve positioning hole 132 expand gradually from the inside outward in a trumpet-shaped pattern. The corresponding rod positioning holes 131 and sleeve positioning holes 132 communicate with each other to form a positioning through-hole that penetrates the docking portion 13, facilitating docking of the rod 20 and sleeve 30. Specifically, the rod 20 passes through the rod positioning holes 131 and contacts or is inserted into the sleeve 30, which is positioned through the sleeve positioning holes 132. To facilitate observation of the docking between the rod 20 and sleeve 30, an opening may be provided above the rod positioning holes 131.

[0031] Combined with reference Figure 6 and Figure 7 In some embodiments of the present invention, the clamp 1 may further include one or more carrier rod support frames 40. The carrier rod support frame 40 has a trough body that can carry one or more carrier rods at the same time. The carrier rod support frame 40 is arranged on the clamp body 10, and it can be installed between the first clamping part 11 and the docking part 13 or between the first clamping part 11 and the second clamping part 12. The carrier rod support frame 40 allows multiple carrier rods to be clamped and fixed by the first clamping part 11 at the same time, allowing multiple carrier rods to be moved together more conveniently, thereby improving work efficiency. It should be noted that the biological tissue transfer device according to the embodiment of the present application may include one or more carrier rod support frames 40, and each carrier rod support frame 40 can be used to carry multiple carrier rods, such as 1-3 carrier rods.

[0032] In an embodiment of the present application, the first clamping portion 11 can be axially moved by electromagnetic force, mechanical force, friction, or other means to release the embryo or other biological tissue held in the groove of the carrier rod 20 into the sleeve 30. Specifically, the clamp 1 may further include a drive device and a reset device (not shown) for achieving axial reciprocating motion of the first clamping portion 11. The drive device can accelerate the movement of the slider 113, thereby driving the first clamping portion 11 away from the second clamping portion 12. In practice, the drive device can be an electromagnet. The reset device is used to generate a reset action. In practice, the reset device can be a motor and a spring to achieve an automatic reset function. Thus, the cooperation of the drive device and the reset device can cause the slider 113 to perform axial reciprocating motion, thereby driving the carrier rod 20 held by the first clamping portion 11 to also perform axial reciprocating motion. The number of reciprocating motions can range from 1 to 100, or 1 to 10. Furthermore, the travel of the front end of the carrier rod is 0.01 mm to 30 mm, or 0.01 mm to 5.5 mm. Furthermore, in some embodiments of the present application, the clamp device may further include a sensor (not shown) for detecting axial movement of the slider 113 relative to the clamp body 10. This sensor can determine whether the slider 113 has completed one axial movement and returned to its original position.

[0033] According to an embodiment of the present application, by providing a first clamping portion and a second clamping portion with opposite ends, the first clamping portion that clamps the carrier rod carrying the biological tissue can move axially relative to the second clamping portion that clamps the sleeve, thereby automatically transferring the biological tissue in the carrier rod to the sleeve. The transfer of biological tissues such as embryos by using this clamping device is simple and has a high success rate, and is particularly suitable for applications in pasture environments (such as cattle); further, by providing a docking portion between the first clamping portion and the second clamping portion, the carrier rod and the sleeve can be accurately aligned, further improving the success rate of biological tissue transfer.

[0034] Although the present disclosure has been described in detail with reference to specific embodiments thereof, those skilled in the art will appreciate that various changes and modifications may be made therein without departing from the spirit and scope of the embodiments. Therefore, the present disclosure is intended to cover modifications and variations of the present disclosure, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure shall be included within the scope of the claims of the present disclosure and their equivalents.

[0035] In addition, the features disclosed in the above description or claims or drawings, in their specific forms or according to the manner for performing the disclosed functions or the method or process for obtaining the disclosed results, can be used alone or in any combination of these features to implement the present invention in their different forms. Specifically, one or more features of any embodiment described herein can be combined with one or more features of any other embodiment described herein.

[0036] Protection may also be sought for any features disclosed in any one or more of the publications cited in conjunction with the present disclosure and / or incorporated by reference.

Claims

1. A clamp for transferring biological tissue, characterized in that: include: a first clamping portion, the first clamping portion having at least one rod clamping groove, a rod clamped in the rod clamping groove, and an end of the rod containing biological tissue; a second clamping portion, the second clamping portion having at least one sleeve clamping groove, wherein the sleeve clamping groove clamps the sleeve; The end of the carrier rod is opposite to the end of the sleeve, and the first clamping portion moves axially to transfer the biological tissue contained in the carrier rod into the sleeve.

2. The clamp according to claim 1, characterized in that Also includes: The docking portion is detachably arranged between the first clamping portion and the second clamping portion. The docking portion has at least one positioning through hole. The carrier rod passes through the positioning through hole so that the end of the carrier rod and the end of the sleeve are opposite to each other.

3. The clamp according to claim 2, characterized in that The positioning through hole includes a rod positioning hole opened toward the first clamping portion and a sleeve positioning hole opened toward the second clamping portion. The rod positioning hole and the sleeve positioning hole are connected and gradually expand from the inside to the outside in a trumpet shape.

4. The clamp according to claim 3, characterized in that An opening is provided above the carrier rod positioning hole.

5. The clamp according to claim 1, wherein: Also includes: One or more rod support frames, each of the rod support frames carries a plurality of rods.

6. The clamp according to claim 1, wherein: The first clamping portion further includes a locking structure for locking the carrier rod in the carrier rod clamping groove.

7. The clamp according to claim 1, wherein: Also includes: A slider is provided below the first clamping portion, and the slider drives the first clamping portion to perform axial movement.

8. The clamp according to claim 1, wherein: The sleeve clamping groove and the carrier rod clamping groove have the same height.

9. The clamp according to claim 1, wherein: The carrier rod is provided with a groove near the front end, and the groove contains liquid containing at least biological tissue.

10. The clamp according to claim 1, wherein: The biological tissue includes an embryo or an egg.

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