Dynamic axial compression column

Abstract

The utility model discloses a dynamic axial compression column, and relates to the field of compression columns. The dynamic axial compression column comprises a heat preservation jacket, a piston plate, a support and a measuring rod, the heat preservation jacket is provided with a compression cavity with an opening in the upper end, and the heat preservation jacket is provided with an interlayer arranged around the compression cavity; the measuring rod and the support are slidably arranged. Circulating liquid for heat exchange circulates through the first inlet, the interlayer and the first outlet on the heat preservation jacket, so that filler in the compression cavity is stabilized at an expected temperature, and the consistency of filler column efficiency is ensured; the piston plate axially compresses the filler, the measuring rod correspondingly moves along with the piston plate, the filling height is correspondingly measured according to the movement of the measuring rod, column filling is stopped when the required length is reached, and it is guaranteed that the filling heights of the materials are the same and consistent every time, so that the column efficiency is accurately calculated; according to the utility model, the use requirements of filler filling height measurement and filler temperature control can be met, and the column efficiency and the column temperature are easier to control.

Description

Technical Field

[0001] The utility model relates to the field of compression columns, in particular to a dynamic axial compression column. Background Art

[0002] Dynamic axial compression columns can be used to pack materials, maintain column pressure, and unload pressure. Dynamic axial compression columns can axially compress materials, allowing them to be stably separated under pressure. Users can determine the actual column efficiency at the desired pressure.

[0003] Currently, the maximum loading height of most dynamic axial compression columns is 650mm, and the actual commonly used height is 250mm. However, since the actual loading height cannot be effectively measured, the weight of the loaded filler is often used to estimate the loading height. If the column is packed with wet filler, it cannot be guaranteed that the material loading height is consistent each time, and the column efficiency cannot be accurately calculated. For simple sample separation, the existing column can be adapted to meet the use requirements. If the loaded material is a complex and difficult to purify polypeptide sample, the separation of the sample and impurities in the column requires a stable temperature and a higher temperature, such as above 40°C. However, the temperature in the existing column is easily disturbed by the heat exchange between the mobile phase temperature and the ambient temperature, which affects the consistency of the column efficiency of the filler and cannot meet the requirements of reliable use. Utility Model Content

[0004] In order to solve the technical problems raised by the above background technology, the present invention provides a dynamic axial compression column, comprising:

[0005] A thermal insulation jacket having a compression chamber with an upper opening, an interlayer disposed around the compression chamber within the thermal insulation jacket, the interlayer being suitable for circulating a circulating fluid for heat exchange, the interlayer being thermally connected to the compression chamber, and a first inlet and a first outlet being configured on the thermal insulation jacket and communicating with the interlayer, the first inlet and the first outlet being respectively adapted to be connected to an external circulating fluid pipeline;

[0006] a piston plate, the piston plate being slidably disposed in the compression chamber along an extension direction of the compression chamber;

[0007] A bracket is arranged to be spaced apart from the piston plate;

[0008] And a measuring rod, the measuring rod and the bracket are slidably configured, and the sliding direction of the measuring rod is arranged parallel to the sliding direction of the piston plate; one end of the measuring rod is detachably connected to the side of the piston plate away from the compression chamber where the force is applied, and the other end of the measuring rod is extended away from the piston plate.

[0009] As an optimal technical solution, a mounting portion is provided on the upper end of the piston plate, and the mounting portion and the measuring rod are detachably connected; a guide portion is provided on the bracket, and the guide portion and the measuring rod are slidingly configured; the mounting portion and the guide portion are relatively aligned along the sliding direction of the piston plate.

[0010] As a preferred technical solution, the compression chamber is configured as a cylindrical structure, the piston plate is configured as a cylindrical structure, and the outer peripheral wall surface of the piston plate is sealed against the inner wall surface of the compression chamber.

[0011] As a preferred technical solution, the measuring rod is configured as a cylindrical rod scale, the mounting portion is configured as a circular groove structure, the guide portion is configured as a circular through hole structure, and the circular groove structure and the circular through hole structure are coaxially arranged.

[0012] As a preferred technical solution, a second inlet is provided on the piston plate, the second inlet passes through the piston plate, and the second inlet is communicated with the compression chamber.

[0013] As a preferred technical solution, the thermal insulation jacket is provided with a second outlet, the second outlet is isolated from the interlayer, and the second outlet is connected to the compression chamber.

[0014] As a preferred technical solution, the thermal insulation jacket is configured as a rotating body structure, the thermal insulation jacket has a lateral wall and a bottom wall, the lateral wall and the bottom wall are fixedly connected, the sandwich structure is arranged in the lateral wall, the first inlet and the first outlet are arranged on the outer peripheral side of the lateral wall, the second outlet is arranged on the bottom wall, and the second outlet is arranged through the bottom wall to communicate with the compression chamber.

[0015] As a preferred technical solution, the compression chamber is extended in the height direction, and the inlet height of the first inlet is higher than the outlet height of the first outlet.

[0016] The technical solution provided by the utility model has the following advantages:

[0017] The utility model provides a dynamic axial compression column, including a thermal insulation jacket, a piston plate, a bracket and a measuring rod. The thermal insulation jacket has a compression chamber with an upper opening, and the thermal insulation jacket is provided with an interlayer arranged around the compression chamber, the interlayer is suitable for circulating circulating fluid for heat exchange, the interlayer and the compression chamber are thermally connected, and a first inlet and a first outlet connected to the interlayer are constructed on the thermal insulation jacket, and the first inlet and the first outlet are respectively suitable for being connected to an external circulating fluid pipeline; the piston plate is slidingly arranged in the compression chamber along the extension direction of the compression chamber, the bracket and the piston plate are spaced apart and avoided, the measuring rod and the bracket are slidingly configured, and the sliding direction of the measuring rod is parallel to the sliding direction of the piston plate; one end of the measuring rod is detachably connected to the side of the piston plate away from the force-applying compression chamber, and the other end of the measuring rod is extended away from the piston plate.

[0018] The dynamic axial compression column of this structure circulates the circulating liquid for heat exchange through the first inlet, interlayer, and first outlet on the insulation jacket, so that the filler in the compression chamber is stabilized at the desired temperature, avoiding interference in the heat exchange between the mobile phase temperature and the ambient temperature, so as to reasonably measure the column efficiency and ensure the consistency of the filler column efficiency; after the column is loaded, the piston plate axially compresses the filler, and the measuring rod moves accordingly with the piston plate. The filling height is measured according to the movement of the measuring rod, and the column loading is stopped when the required length is reached. This method can ensure that the height of the material loading is the same each time, so as to accurately calculate the column efficiency; and the measuring rod can be removed from the piston plate and the bracket, which is simple and convenient to operate. The utility model can meet the use requirements of measuring the filler filling height and controlling the filler temperature. The column efficiency and column temperature are easier to control, which is beneficial to the separation and purification of polypeptide products. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 This is a schematic structural diagram of the dynamic axial compression column provided by the utility model;

[0021] Figure 2 This is a schematic structural diagram of the piston plate in the dynamic axial compression column provided by the present invention;

[0022] Figure 3 A schematic diagram of the partial structure of the bracket in the dynamic axial compression column provided by the present invention;

[0023] Description of reference numerals:

[0024] 1-insulation jacket; 11-compression chamber; 12-first inlet; 13-first outlet; 14-second outlet;

[0025] 2-piston plate; 21-second inlet; 22-mounting portion;

[0026] 3- bracket; 31- guide portion;

[0027] 4- measuring rod;

[0028] 5- Filler. DETAILED DESCRIPTION

[0029] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0030] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0031] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0032] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0033] Example

[0034] This embodiment provides a dynamic axial compression column, see Figure 1The apparatus comprises a thermal insulation jacket 1, a piston plate 2, a bracket 3, and a measuring rod 4. The thermal insulation jacket 1 has a compression chamber 11 with an open upper end. The thermal insulation jacket 1 is provided with an interlayer surrounding the compression chamber 11. The interlayer is suitable for circulating a circulating fluid for heat exchange. The interlayer and the compression chamber 11 are thermally connected. The thermal insulation jacket 1 is provided with a first inlet 12 and a first outlet 13 connected to the interlayer. The first inlet 12 and the first outlet 13 are respectively suitable for connecting to an external circulating fluid pipeline. The measuring rod 4 is provided with a scale mark for measuring the filling height.

[0035] In this embodiment, the piston plate 2 is slidably arranged in the compression chamber 11 along the extension direction of the compression chamber 11, the bracket 3 is spaced apart from the piston plate 2, the measuring rod 4 and the bracket 3 are slidably configured, and the sliding direction of the measuring rod 4 is arranged parallel to the sliding direction of the piston plate 2; one end of the measuring rod 4 is detachably connected to the side of the piston plate 2 away from the force-applying compression chamber 11, and the other end of the measuring rod 4 is extended away from the piston plate 2.

[0036] The dynamic axial compression column provided in this embodiment circulates the circulating liquid for heat exchange through the first inlet 12, the interlayer, and the first outlet 13 on the thermal insulation jacket 1, so that the filler 5 in the compression chamber 11 is stabilized at the desired temperature, avoiding interference between the mobile phase temperature and the ambient temperature in the heat exchange, so as to reasonably measure the column efficiency and ensure the consistency of the column efficiency of the filler 5; after the column is loaded, the piston plate 2 axially compresses the filler 5, and the measuring rod 4 moves accordingly with the piston plate 2. The filling height is measured according to the movement of the measuring rod 4, and the column loading is stopped when the required length is reached. In this way, the height of each material loading is the same, so as to accurately calculate the column efficiency; and the measuring rod 4 can be removed from the piston plate 2 and the bracket 3, which is simple and convenient to operate. The utility model can meet the use requirements of measuring the filling height of the filler 5 and controlling the temperature of the filler 5, and the column efficiency and column temperature are easier to control, which is beneficial to the separation and purification of polypeptide products.

[0037] In some embodiments, the insulation jacket 1 is connected to an external circulation device, specifically, the input side of the first inlet 12 is connected to the circulating liquid pipeline, and the output side of the first outlet 13 is connected to the circulating liquid pipeline. The external circulation device is equipped with a driving pump and a water tank. The driving pump is used to drive the circulating liquid in the water tank to be diverted to the first inlet 12 through the circulating liquid pipeline, and to recover the circulating liquid flowing out of the first outlet 13 into the water tank through the circulating liquid pipeline. The water tank is equipped with a temperature control device, and the circulating liquid is heated or cooled by the temperature control device to meet the heat exchange requirements of different expected temperatures of the compression chamber 11.

[0038] In some embodiments, the piston plate 2 is connected to an external driving mechanism, which drives the relative sliding of the piston plate 2 and the compression chamber 11 and drives the measuring rod 4 to move.

[0039] In a specific embodiment, a temperature detection element may be provided in the interlayer of the thermal insulation jacket 1 to detect the temperature of the circulating fluid in the interlayer.

[0040] In this embodiment, see Figure 2 and Figure 3 The piston plate 2 has a mounting portion 22 at its upper end, which is detachably connected to the measuring rod 4. The bracket 3 has a guide portion 31, which is slidably disposed with the measuring rod 4. The mounting portion 22 and the guide portion 31 are arranged in a symmetrical arrangement relative to each other along the sliding direction of the piston plate 2. The mounting portion 22 is connected to the end of the measuring rod 4, and the guide portion 31 slidably guides the measuring rod 4. This improves the accuracy of the measuring rod 4's movement with the piston plate 2, ensuring that the measuring rod 4 remains in place and is less likely to deviate as the piston plate 2 descends to compress the packing 5.

[0041] In a specific embodiment, the compression chamber 11 is configured as a cylindrical structure, the piston plate 2 is configured as a cylindrical structure, and the outer peripheral wall surface of the piston plate 2 is sealed against the inner wall surface of the compression chamber 11 .

[0042] In some embodiments, see Figures 1 to 3 The measuring rod 4 is set as a cylindrical rod scale, the mounting portion 22 is set as a circular groove structure, the guide portion 31 is set as a circular through hole structure, and the circular groove structure and the circular through hole structure are coaxially arranged.

[0043] In a specific embodiment, a clamping groove may be provided in the circular groove structure, and a clamping protrusion is provided at the end of the measuring rod 4, and the clamping protrusion and the clamping groove are clamped to each other so that the piston plate 2 is connected to the measuring rod 4. The clamping protrusion on the measuring rod 4 can be clamped in the clamping groove by rotation, and a stop edge is provided on the clamping groove; specifically, the clamping protrusion is first inserted into the clamping groove, and then the clamping protrusion is rotated, and the stop edge limits the axial movement between the clamping protrusion and the clamping groove, so that the piston plate 2 drives the measuring rod 4 to move axially; when the piston plate 2 and the measuring rod 4 need to be separated, the measuring rod 4 is rotated to reset the clamping protrusion to the initial position of the insertion into the clamping groove, and then the measuring rod 4 is moved axially away from the piston plate 2.

[0044] In other embodiments, the circular groove structure may be provided with a threaded connection hole, and the end of the measuring rod 4 may be provided with a threaded connection protrusion, and the piston plate 2 and the measuring rod 4 may be connected or separated through the threaded connection hole and the threaded connection protrusion.

[0045] As a further embodiment, see Figure 1The piston plate 2 is provided with a second inlet 21, which extends through the piston plate 2 and is in communication with the compression chamber 11. The thermal insulation jacket 1 is provided with a second outlet 14, which is isolated from the interlayer and is in communication with the compression chamber 11. The second inlet 21 is used for the inlet of the mobile phase and the sample, and the second outlet 14 is used for the outlet of the mobile phase and the sample.

[0046] In a specific embodiment, the insulation jacket 1 is configured as a rotating body structure, the insulation jacket 1 has a side wall and a bottom wall, the side wall and the bottom wall are fixedly connected, the sandwich structure is arranged in the side wall, the first inlet 12 and the first outlet 13 are arranged on the outer peripheral side of the side wall, the second outlet 14 is arranged on the bottom wall, and the second outlet 14 is arranged through the bottom wall to communicate with the compression chamber 11.

[0047] In this embodiment, one or more measuring rods 4 are provided. For two or more measuring rods 4 , the measuring rods 4 are distributed on the piston plate 2 in an annular shape.

[0048] As a preferred embodiment, see Figure 1 The compression chamber 11 is extended in the height direction, and the inlet height of the first inlet 12 is higher than the outlet height of the first outlet 13. This arrangement allows the circulating fluid to circulate from top to bottom in the interlayer.

[0049] In some embodiments, electrically controlled valves may be installed at the first inlet 12 and the first outlet 13, respectively, to adjust the flow rate and flow volume of the circulating liquid in the interlayer.

[0050] The dynamic axial compression column provided in this embodiment can be specifically configured as DAC50, DAC100 or DAC200.

[0051] The dynamic axial compression column provided by the utility model is used as follows:

[0052] Before installing the column, ensure that the piston plate 2 is directly above the lateral wall of the insulation jacket 1. Then, pass the measuring rod 4 through the guide portion 31 of the bracket 3 and connect it to the mounting portion 22 of the piston plate 2. At this time, the operator records the scale value where the upper side of the measuring rod 4 intersects with the lower edge of the bracket 3 as the initial reading.

[0053] Next, the pre-prepared wet filler 5 can be slowly poured into the compression chamber 11 to implement column packing;

[0054] Then, the piston plate 2 is driven downward along the inner wall of the compression chamber 11 to compress the entire packing 5. During the compression process, the scale changes on the measuring rod 4 are continuously observed. When the scale value is about to reach the preset target value, the movement of the piston plate 2 is immediately stopped.

[0055] By calculating the difference between the initial scale value and the final scale value, the column height can be accurately obtained; after the column packing process is completed, the measuring rod 4 is removed to prevent subsequent purification and column unpacking operations from damaging the measuring rod 4 or affecting its scale;

[0056] Afterwards, the first inlet 12 and the second inlet 21 can be connected to the circulation equipment through the circulating liquid pipeline, the circulating liquid temperature can be set on the circulating equipment, and the circulation can be started to stabilize the circulating liquid temperature in the interlayer within the required range of the product purification process; then, the second inlet 21 and the second outlet 14 can be connected to the preparative liquid phase pump and the detector respectively, and the preparative liquid phase pump unit can be turned on to allow the sample and the mobile phase to enter the compression chamber 11 in sequence. Under the specified column temperature conditions, the product and impurities in the sample are effectively separated by the chromatographic action of the column filler 5. The separated product and impurities flow out from the mobile phase and sample outlets of the column in sequence along with the mobile phase and enter the preparative liquid phase detection unit for fraction collection.

[0057] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A dynamic axial compression column, characterized in that: include: A heat-insulating jacket (1) having a compression chamber (11) with an upper opening, an interlayer arranged around the compression chamber (11) in the heat-insulating jacket (1), the interlayer being suitable for circulating a circulating fluid for heat exchange, the interlayer and the compression chamber (11) being heat-conductively connected, a first inlet (12) and a first outlet (13) being configured on the heat-insulating jacket (1) and communicating with the interlayer, the first inlet (12) and the first outlet (13) being respectively suitable for connection to an external circulating fluid pipeline; a piston plate (2), the piston plate (2) being slidably disposed in the compression chamber (11) along an extension direction of the compression chamber (11); The bracket (3) is arranged to be spaced apart from the piston plate (2); And a measuring rod (4), the measuring rod (4) and the bracket (3) are slidably arranged, and the sliding direction of the measuring rod (4) is arranged parallel to the sliding direction of the piston plate (2); one end of the measuring rod (4) is detachably connected to the side of the piston plate (2) away from the compression chamber (11) where the force is applied, and the other end of the measuring rod (4) is extended away from the piston plate (2).

2. The dynamic axial compression column according to claim 1, characterized in that The upper end of the piston plate (2) is provided with a mounting portion (22), and the mounting portion (22) and the measuring rod (4) are detachably connected; the bracket (3) is provided with a guide portion (31), and the guide portion (31) and the measuring rod (4) are slidably arranged relative to each other; the mounting portion (22) and the guide portion (31) are relatively aligned along the sliding direction of the piston plate (2).

3. The dynamic axial compression column according to claim 2, characterized in that: The compression chamber (11) is configured as a cylindrical structure, the piston plate (2) is configured as a cylindrical structure, and the outer peripheral wall surface of the piston plate (2) is sealed against the inner wall surface of the compression chamber (11).

4. The dynamic axial compression column according to claim 3, characterized in that: The measuring rod (4) is configured as a cylindrical rod scale, the mounting portion (22) is configured as a circular groove structure, the guide portion (31) is configured as a circular through hole structure, and the circular groove structure and the circular through hole structure are coaxially arranged.

5. The dynamic axial compression column according to any one of claims 1 to 4, characterized in that: A second inlet (21) is provided on the piston plate (2), the second inlet (21) passes through the piston plate (2), and the second inlet (21) is communicated with the compression chamber (11).

6. The dynamic axial compression column according to claim 5, characterized in that: The heat-insulating jacket (1) is provided with a second outlet (14), the second outlet (14) is isolated from the interlayer, and the second outlet (14) is connected to the compression chamber (11).

7. The dynamic axial compression column according to claim 6, characterized in that: The thermal insulation jacket (1) is configured as a rotating body structure, and the thermal insulation jacket (1) has a lateral wall body and a bottom wall body, the lateral wall body and the bottom wall body are fixedly connected, the sandwich structure is arranged in the lateral wall body, the first inlet (12) and the first outlet (13) are arranged on the outer peripheral side of the lateral wall body, the second outlet (14) is arranged on the bottom wall body, and the second outlet (14) is arranged through the bottom wall body to communicate with the compression chamber (11).

8. The dynamic axial compression column according to any one of claims 1 to 4, characterized in that: The compression chamber (11) is extended in a height direction, and the inlet height of the first inlet (12) is higher than the outlet height of the first outlet (13).

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