Rapid fixing device for biological tissues and cells

Abstract

The utility model relates to the field of clinical and research pathology, in particular to a rapid fixing device for biological tissues and cells, which is used for fixing tissues / cells and comprises a first container, a second container and a third container, the ultrasonic / microwave transmitter is fixed on the side wall of the first container and transmits ultrasonic waves / microwaves to the accommodating space; and the stationary liquid is positioned in the accommodating space and is used for soaking tissues / cells. The first container is arranged to define the containing space, the containing space is used for containing stationary liquid and tissue / cells, the ultrasonic / microwave emitter is fixedly arranged on the side wall of the first container, and the stationary liquid can vibrate through ultrasonic waves or microwaves emitted by the ultrasonic / microwave emitter; therefore, the fixing speed of tissues / cells is accelerated, and the fixing time is shortened. The utility model has the advantages of simple structure and lower cost, can quickly fix tissues / cells, and has important application in the fields of pathological research, gene sequencing, immunohistochemical staining and the like.

Description

Technical Field

[0001] The utility model relates to the field of clinical and research pathology, in particular to a biological tissue and cell rapid fixation device. Background Art

[0002] Tissue fixation is a crucial step in histopathology. It involves using various methods to restore the cellular contents of a tissue, after removal from the body, to their original morphology, structure, and composition, excluding water, as closely as possible to their original state. Cell fixation is the process of affixing cells to histological sections, slides, or other matrices, aiming to preserve their original morphology and structure for easier observation and experimental manipulation.

[0003] Tissue / cell fixation is a key step in pathology and biology, and is of great significance for subsequent testing and research.

[0004] However, currently, most methods of fixing tissues / cells directly with fixatives have the disadvantage of long fixation times, which may lead to untimely fixation and subsequent tissue / cell autolysis or corruption. Utility Model Content

[0005] The purpose of the utility model is to overcome the disadvantages of the prior art that most of the fixatives are directly used to fix tissues / cells, resulting in a long fixation time, and to provide a rapid fixation device for biological tissues and cells.

[0006] The utility model provides a biological tissue and cell fast fixation device for fixing tissues / cells, comprising

[0007] A first container encloses a holding space;

[0008] an ultrasonic / microwave transmitter, fixed to a side wall of the first container, and transmitting ultrasonic / microwaves toward the containing space;

[0009] The fixative is located in the containing space and is used for soaking tissues / cells.

[0010] The present invention forms a holding space by providing a first container to enclose a holding space for placing a fixative solution and tissues / cells (i.e., cells or tissues). An ultrasonic / microwave emitter (i.e., an instrument capable of generating ultrasonic waves or microwaves) is then fixed to the sidewall of the first container. The ultrasonic / microwave emitter emits ultrasonic waves or microwaves that vibrate the fixative solution, thereby accelerating the fixation of the tissues / cells and shortening the fixation time. Furthermore, the present invention directly secures the ultrasonic / microwave emitter to the sidewall of the first container and emits ultrasonic waves / microwaves toward the holding space, reducing the loss of ultrasonic waves / microwaves during transmission, further accelerating the fixation of the tissues / cells and shortening the fixation time.

[0011] Preferably, the first container contains liquid. By containing the liquid in the first container, the tissue / cells can float in the first container, thereby facilitating the fixation of the tissue / cells.

[0012] Preferably, the liquid is water or the stationary liquid.

[0013] Preferably, the rapid fixation device for biological tissues and cells further comprises a second container, which is placed within the holding space, and the tissues / cells are placed within the second container. In some embodiments, the provision of the second container allows for use-and-disposal of the second container, thereby achieving single-use and preventing contamination of the tissues / cells.

[0014] Preferably, the second container is a glass tank, a glass cup or a plastic bag.

[0015] Preferably, the second container floats in the accommodating space, thereby achieving tissue / cell floating, which facilitates the fixation of the tissue / cell.

[0016] Preferably, the plurality of ultrasonic / microwave transmitters are evenly arranged along the outer wall of the first container, which can improve the uniformity of fixation and enhance the fixation effect.

[0017] Preferably, the cross section of the first container is circular, which is more convenient for arranging the ultrasonic / microwave emitters, so that multiple ultrasonic / microwave emitters can be symmetrically arranged, further improving the uniformity of ultrasonic / microwave energy in the accommodation space, which is beneficial to improving the fixing effect.

[0018] Preferably, the first container includes a cup body and a cover body, wherein the cup body encloses the accommodation space and the cover body is used to seal the accommodation space. By providing the cover body, volatilization of the fixing liquid can be reduced, thereby reducing air pollution.

[0019] Preferably, the fixative includes formaldehyde fixative, ethanol fixative, or ethanol-formaldehyde fixative.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention forms a holding space by providing a first container, which is used to house a fixative solution and tissues / cells. An ultrasonic / microwave transmitter is then fixed to the sidewall of the first container. The ultrasonic / microwave transmitter emits ultrasonic or microwave waves, which vibrate the fixative solution, thereby accelerating the fixation of the tissues / cells and shortening the fixation time. Furthermore, the present invention directly secures the ultrasonic / microwave transmitter to the sidewall of the first container and transmits ultrasonic / microwave waves toward the holding space, reducing ultrasonic / microwave losses during transmission, further accelerating the fixation of the tissues / cells and shortening the fixation time.

[0022] 2. The utility model has a simple structure and low cost, can accelerate the fixation of tissues / cells, and can quickly achieve tissue / cell fixation. It has important applications in pathological research, gene sequencing, immunohistochemical staining and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a front view of the biological tissue and cell rapid fixation device described in Example 1 of the present utility model.

[0024] Figure 2 for Figure 1 AA cross-sectional view.

[0025] Figure 3 This is a front view of the biological tissue and cell rapid fixation device described in Example 2 of the present utility model.

[0026] Figure 4 for Figure 3 BB cross-sectional view.

[0027] Figure 5 This is a front view of the biological tissue and cell rapid fixation device described in Example 3 of the present utility model.

[0028] Figure 6 for Figure 5 CC cross-sectional view.

[0029] Markings in the figure:

[0030] 1-first container, 11-cup body, 12-cover body,

[0031] 2-Ultrasonic / microwave transmitter;

[0032] 3-Liquid,

[0033] 4- Second container,

[0034] 5-Fixative solution,

[0035] 6-Tissue / cells. DETAILED DESCRIPTION

[0036] The present invention will be further described in detail below with reference to specific embodiments. However, this should not be construed as limiting the scope of the present invention to the following embodiments. All technologies implemented based on the present invention fall within the scope of the present invention.

[0037] Unless otherwise specified, in the description of the specific embodiments of the present invention, the terms indicating orientation or positional relationships, such as "upper," "lower," "left," "right," "center," "inside," and "outside," are based on the orientation or positional relationships shown in the accompanying drawings, or are the orientation or positional relationships in which the product / device / apparatus of the present invention is typically placed when in use. These terms indicating orientation or positional relationships are merely for the purpose of facilitating the description of the present invention or simplifying the description of the specific embodiments to facilitate a quick understanding of the solutions by technicians. They do not indicate or imply that a particular device / component / element must have a specific orientation or be constructed and operated in a specific positional relationship, and therefore should not be construed as limiting the present invention.

[0038] In addition, if the terms "horizontal", "vertical", "overhanging", "parallel" and the like appear, it does not mean that the corresponding devices / components / elements are required to be absolutely horizontal or vertical or overhanging or parallel, but may be slightly tilted or have deviations. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but may be slightly tilted. Alternatively, it can be simply understood that the corresponding devices / components / elements are set in directions such as "horizontal", "vertical", "overhanging", and "parallel", and can have an error / deviation of ±10% relative to the corresponding direction setting, more preferably an error / deviation within ±8%, more preferably an error / deviation within ±6%, more preferably an error / deviation within ±5%, and more preferably an error / deviation within ±4%. As long as the corresponding device / component / element is within the error / deviation range, it can still achieve its role in the solution of the present utility model.

[0039] In addition, the expressions "first", "second", "third", etc. that appear in the terms are merely descriptions used to distinguish the same or similar components and should not be understood as emphasizing or implying the relative importance of specific components.

[0040] In addition, in the description of the embodiments of the present invention, "several", "a plurality", and "a number" represent at least 2. It can be any number such as 2, 3, 4, 5, 6, 7, 8, 9, and even more than 9.

[0041] Furthermore, in the description of the technical solutions of this utility model, unless otherwise expressly specified / defined / restricted, the terms "disposed," "installed," "connected," "connected," "provided with," "laid," and "arranged" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections. They may be welding, riveting, bolting, threading, or other commonly used connection methods in the art. Such connections may be mechanical, electrical, or communication connections; they may be direct connections, indirect connections through an intermediate medium, or internal connections between two components.

[0042] Example 1

[0043] like Figure 1-2 As shown, a biological tissue and cell rapid fixation device is used to fix tissues / cells 6. The biological tissue and cell rapid fixation device includes a first container 1, an ultrasonic / microwave transmitter 2, and a fixative 5.

[0044] The first container 1 encloses a storage space, and the ultrasonic / microwave emitter 2 is fixed to the sidewall of the first container 1. The ultrasonic / microwave emitter 2 emits ultrasonic waves / microwaves toward the storage space. The fixative 5 is used to immerse tissues / cells 6, and the fixative 5 is located within the storage space. Therefore, the ultrasonic waves or microwaves emitted by the ultrasonic / microwave emitter 2 can cause the fixative 5 to vibrate, thereby accelerating the fixation of the tissues / cells 6 by the fixative 5, thereby achieving rapid fixation of the tissues and cells.

[0045] The term "rapid" in the rapid fixation device for biological tissues and cells described herein means that, compared to existing methods of fixing tissues and cells using only a fixative 5, the device provided herein can accelerate the fixation speed of the fixative 5 on the tissues / cells 6. The term "rapid" in the rapid fixation device for biological tissues and cells described herein does not refer to a specific speed range. Those skilled in the art will appreciate that the fixation speed of tissues and cells is significantly affected by the size of the tissues and cells, the type of fixative used, and other factors. Therefore, it is difficult to define a reasonable fixation speed range.

[0046] In this embodiment, the fixative 5 is placed directly in the first container 1, and the tissue / cells 6 are directly immersed in the fixative 5. Thus, the ultrasonic / microwave emitter 2 is placed against the outer wall of the first container 1, and the ultrasonic / microwaves emitted by the ultrasonic / microwave emitter 2 act on the fixative 5, thereby causing the fixative 5 to vibrate. In alternative embodiments, the ultrasonic / microwave emitter 2 can be directly adhered to the outer wall of the first container 1, or snap-fitted to the outer wall of the first container 1, or a placement platform is provided on the outer wall of the first container 1, and the ultrasonic / microwave emitter 2 is fixed to the placement platform, etc.

[0047] To further improve the fixation speed, multiple ultrasonic / microwave emitters 2 can be provided, for example, 2-10. These multiple ultrasonic / microwave emitters 2 can be evenly distributed along the outer wall of the first container 1, thereby generating a relatively uniform energy distribution within the first container 1 and improving the fixation effect. More preferably, the cross-section of the first container 1 can be configured to be circular, which further facilitates the uniform distribution of the ultrasonic / microwave emitters 2 and further improves the uniformity of energy distribution within the first container 1.

[0048] In an optional embodiment, the fixative 5 includes formaldehyde fixative, ethanol fixative, ethanol-formaldehyde fixative, etc.

[0049] In an optional embodiment, the fixative 5 can soak only one tissue / cell 6 at a time, or can soak multiple tissues / cells 6 at the same time.

[0050] In an optional embodiment, the first container 1 may include a cup body 11 and a cover body 12. The cup body 11 encloses the storage space, and the cover body 12 is used to seal the storage space, thereby reducing the volatilization of the fixing liquid 5 and reducing air pollution. In a further preferred embodiment, the cup body 11 may be cylindrical, and the cover body 12 may be a circular cover plate.

[0051] Example 2

[0052] like Figure 3-4 As shown, a biological tissue and cell rapid fixation device is used to fix tissue / cells 6. The biological tissue and cell rapid fixation device includes a first container 1, an ultrasonic / microwave transmitter 2, a liquid 3, a second container 4, and a fixative 5.

[0053] The first container 1 encloses a containing space, the ultrasonic / microwave transmitter 2 is fixed to the side wall of the first container 1, and the ultrasonic / microwave transmitter 2 transmits ultrasonic / microwave toward the containing space.

[0054] The first container 1 contains the liquid 3 , that is, the liquid 3 is located in the containing space. The liquid 3 may be a stationary liquid or other liquids, for example, the liquid 3 may be water.

[0055] The second container 4 is placed in the accommodation space. In this embodiment, the second container 4 can float on the liquid 3, that is, the second container 4 can float in the accommodation space.

[0056] The second container 4 is filled with a fixative 5 , and the tissue / cell 6 is placed in the second container 4 and immersed in the fixative 5 .

[0057] The ultrasonic wave or microwave emitted by the ultrasonic wave / microwave transmitter 2 can make the liquid 3 and the fixative 5 vibrate, thereby accelerating the fixation speed of the fixative 5 on the tissue / cell 6, thereby achieving rapid fixation of the tissue and cells.

[0058] The term "rapid" in the rapid fixation device for biological tissues and cells described herein means that, compared to existing methods of fixing tissues and cells using only a fixative 5, the device provided herein can accelerate the fixation speed of the fixative 5 on the tissues / cells 6. The term "rapid" in the rapid fixation device for biological tissues and cells described herein does not refer to a specific speed range. Those skilled in the art will appreciate that the fixation speed of tissues and cells is significantly affected by the size of the tissues and cells, the type of fixative used, and other factors. Therefore, it is difficult to define a reasonable fixation speed range.

[0059] In an optional embodiment, the second container 4 may be a glass trough, a glass cup, or a plastic bag. In this embodiment, the second container 4 is a plastic bag.

[0060] In this embodiment, the fixative 5 is directly contained in the second container 4. This second container 4 allows for easy use and disposal, making it disposable. This prevents tissue / cell contamination and satisfies the single-use requirement for medical applications. Furthermore, the first container 1 contains the liquid 3, which acts as a good propagation medium, effectively transmitting the ultrasonic / microwave waves to the second container 4 and further to the fixative 5, thereby increasing fixation speed and achieving a better fixation effect.

[0061] In this embodiment, the ultrasonic / microwave emitter 2 is placed against the outer wall of the first container 1. The ultrasonic / microwave emitted by the ultrasonic / microwave emitter 2 acts on the liquid 3, thereby causing the stationary liquid 5 to vibrate. In alternative embodiments, the ultrasonic / microwave emitter 2 can be directly adhered to the outer wall of the first container 1, or fixed to the outer wall of the first container 1 by snap-fitting, or a placement platform is provided on the outer wall of the first container 1, and the ultrasonic / microwave emitter 2 is fixed to the placement platform, etc.

[0062] To further improve the fixation speed, multiple ultrasonic / microwave emitters 2 can be provided, for example, 2-10. These multiple ultrasonic / microwave emitters 2 can be evenly distributed along the outer wall of the first container 1, thereby generating a relatively uniform energy distribution within the first container 1 and improving the fixation effect. More preferably, the cross-section of the first container 1 can be configured to be circular, which further facilitates the uniform distribution of the ultrasonic / microwave emitters 2 and further improves the uniformity of energy distribution within the first container 1.

[0063] In an optional embodiment, the fixative 5 includes formaldehyde fixative, ethanol fixative, ethanol-formaldehyde fixative, etc.

[0064] In an optional embodiment, the fixative 5 can soak only one tissue / cell 6 at a time, or can soak multiple tissues / cells 6 at the same time.

[0065] In an optional embodiment, the first container 1 may include a cup body 11 and a cover body 12. The cup body 11 encloses the storage space, and the cover body 12 is used to seal the storage space, thereby reducing the volatilization of the fixing liquid 5 and reducing air pollution. In a further preferred embodiment, the cup body 11 may be cylindrical, and the cover body 12 may be a circular cover plate.

[0066] Example 3

[0067] like Figure 5-6 As shown, a biological tissue and cell rapid fixation device is used to fix tissue / cells 6. The biological tissue and cell rapid fixation device includes a first container 1, an ultrasonic / microwave transmitter 2, a second container 4, and a fixative 5.

[0068] The first container 1 encloses a containing space, the ultrasonic / microwave transmitter 2 is fixed to the side wall of the first container 1, and the ultrasonic / microwave transmitter 2 transmits ultrasonic / microwave toward the containing space.

[0069] The second container 4 is placed in the accommodation space. In this embodiment, the second container 4 is located in the first container 1.

[0070] The second container 4 is filled with a fixative 5 , and the tissue / cell 6 is placed in the second container 4 and immersed in the fixative 5 .

[0071] The ultrasonic wave or microwave emitted by the ultrasonic wave / microwave transmitter 2 can cause the fixative 5 to vibrate, thereby accelerating the fixation speed of the fixative 5 on the tissue / cell 6, thereby achieving rapid fixation of the tissue and cells.

[0072] The term "rapid" in the rapid fixation device for biological tissues and cells described herein means that, compared to existing methods of fixing tissues and cells using only a fixative 5, the device provided herein can accelerate the fixation speed of the fixative 5 on the tissues / cells 6. The term "rapid" in the rapid fixation device for biological tissues and cells described herein does not refer to a specific speed range. Those skilled in the art will appreciate that the fixation speed of tissues and cells is significantly affected by the size of the tissues and cells, the type of fixative used, and other factors. Therefore, it is difficult to define a reasonable fixation speed range.

[0073] In an optional embodiment, the second container 4 may be a cup, and further may be a glass.

[0074] In this embodiment, the fixative 5 is placed directly in the second container 4. This second container 4 allows for easy use and disposal, making it disposable, thereby preventing tissue / cell contamination and meeting the single-use requirement for medical applications. Furthermore, to reduce losses during ultrasonic / microwave transmission, the second container 4 should be placed as close as possible to the sidewall of the first container 1. This allows the ultrasonic / microwaves emitted by the ultrasonic / microwave emitter 2 to effectively affect the fixative 5, causing it to vibrate.

[0075] In an optional embodiment, the ultrasonic / microwave emitter 2 is placed against the outer wall of the first container 1. The ultrasonic / microwave emitter 2 can be directly adhered and fixed to the outer wall of the first container 1, or snap-fitted and fixed to the outer wall of the first container 1, or a placement platform is provided on the outer wall of the first container 1, and the ultrasonic / microwave emitter 2 is fixed to the placement platform, etc.

[0076] To further improve the fixation speed, multiple ultrasonic / microwave emitters 2 can be provided, for example, 2-10. These multiple ultrasonic / microwave emitters 2 can be evenly distributed along the outer wall of the first container 1, thereby generating a relatively uniform energy distribution within the first container 1 and improving the fixation effect. More preferably, the cross-section of the first container 1 can be configured to be circular, which further facilitates the uniform distribution of the ultrasonic / microwave emitters 2 and further improves the uniformity of energy distribution within the first container 1.

[0077] In an optional embodiment, the fixative 5 includes formaldehyde fixative, ethanol fixative, ethanol-formaldehyde fixative, etc.

[0078] In an optional embodiment, the fixative 5 can soak only one tissue / cell 6 at a time, or can soak multiple tissues / cells 6 at the same time.

[0079] In an optional embodiment, the first container 1 may include a cup body 11 and a cover body 12. The cup body 11 encloses the storage space, and the cover body 12 is used to seal the storage space, thereby reducing the volatilization of the fixing liquid 5 and reducing air pollution. In a further preferred embodiment, the cup body 11 may be cylindrical, and the cover body 12 may be a circular cover plate.

[0080] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A biological tissue and cell rapid fixation device, characterized in that: For fixation of tissues / cells (6), including A first container (1) encloses a receiving space; An ultrasonic / microwave transmitter (2) is fixed to the side wall of the first container (1) and transmits ultrasonic / microwave toward the containing space; A fixative (5) is located in the receiving space and is used to soak the tissue / cells (6).

2. A biological tissue and cell rapid fixation device according to claim 1, characterized in that: The first container (1) contains liquid (3).

3. A biological tissue and cell rapid fixation device according to claim 2, characterized in that: The liquid (3) is water or the stationary liquid (5).

4. A biological tissue and cell rapid fixation device according to claim 1, characterized in that: It also includes a second container (4), the second container (4) is placed in the containing space, and the tissue / cell (6) is placed in the second container (4).

5. A biological tissue and cell rapid fixation device according to claim 4, characterized in that: The second container (4) is a glass tank, a glass cup or a plastic bag.

6. A biological tissue and cell rapid fixation device according to claim 5, characterized in that: The second container (4) floats in the accommodating space.

7. A biological tissue and cell rapid fixation device according to claim 1, characterized in that: The plurality of ultrasonic / microwave transmitters (2) are evenly arranged along the outer wall of the first container (1).

8. A biological tissue and cell rapid fixation device according to claim 7, characterized in that: The cross section of the first container (1) is circular.

9. A biological tissue and cell rapid fixation device according to any one of claims 1 to 8, characterized in that: The first container (1) comprises a cup body (11) and a cover body (12); the cup body (11) encloses the accommodating space, and the cover body (12) is used to close the accommodating space.

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