Microwave digestion device for measuring organic selenium in animals by atomic fluorescence method

Abstract

The utility model relates to the technical field of detection devices, in particular to a microwave digestion device for measuring organic selenium in animals by an atomic fluorescence method, which comprises a box body, the box body comprises a depressurization chamber and a digestion chamber, the depressurization chamber is separated from the digestion chamber through a partition plate, and a vacuum negative pressure pump is mounted in the depressurization chamber. A controller is arranged in the depressurization chamber, a touch screen is arranged at the front end of the depressurization chamber, the controller is in two-way signal connection with the touch screen, a microwave source is arranged at the top of the digestion chamber, a test tube tray is mounted at the bottom of the digestion chamber, and a chamber door is rotatably mounted at the front end of the digestion chamber; a door groove clamped with the chamber door is formed in the front end of the digestion chamber, a sealing ring groove is formed in the front end surface of the door groove, and a sealing ring is clamped in the sealing ring groove. According to the device disclosed by the utility model, the boiling point of a substance is reduced through uniform heating and a negative pressure environment provided by the microwave source, so that the digestion process can be carried out at a lower temperature, and the digestion efficiency of an animal sample is improved when organic selenium in an animal is measured by an atomic fluorescence method.

Description

Technical Field

[0001] The utility model relates to the technical field of detection devices, in particular to a microwave digestion device for determining organic selenium in animals using an atomic fluorescence method. Background Art

[0002] High-performance liquid chromatography (HPLC)-hydride generation (HG)-atomic fluorescence spectrometry (AFS) is an advanced analytical technique used to determine organic selenium content in animals. Optimization of this method involves multiple aspects, including sample pretreatment, chromatographic conditions, HG conditions, and AFS detection conditions. Sample pretreatment is a critical step in determining organic selenium content in animals. Digestion is a crucial step in converting organic selenium in the sample into a measurable form. Common digestion methods include wet digestion and microwave digestion. The choice of digestion method depends on the specific properties of the sample (such as matrix complexity and selenium speciation distribution) and experimental conditions. Wet digestion, typically performed with strong acids (such as nitric acid and perchloric acid), offers the advantages of simplicity and low cost, but may introduce significant contamination and pose certain risks to the operator. Microwave digestion offers the advantages of uniform heating, rapid digestion, and minimal contamination, but the equipment cost is higher.

[0003] During the digestion process, it is necessary to ensure that the various forms of selenium in the sample (such as selenoproteins, selenoamino acids, etc.) are completely converted into measurable tetravalent selenium (Se^4+). This requires controlling the digestion temperature, time, and acidity. However, most of the currently available microwave digestion devices are difficult to achieve uniform and efficient digestion of animal samples at relatively low temperatures. Therefore, there is an urgent need for a new type of microwave digestion device for atomic fluorescence determination of organic selenium in animals to provide an effective solution to the shortcomings of the existing technology. Utility Model Content

[0004] The purpose of the utility model is to provide a microwave digestion device for determining organic selenium in animals by atomic fluorescence method, so as to solve the problems raised in the above background technology.

[0005] To achieve the above objectives, the present invention provides the following technical solutions:

[0006] A microwave digestion device for determining organic selenium in animals by atomic fluorescence method comprises a housing, the housing comprising a decompression chamber and a digestion chamber, the decompression chamber and the digestion chamber being separated by a partition, a vacuum negative pressure pump being installed in the decompression chamber, the air inlet end of the vacuum negative pressure pump being connected to the digestion chamber through a pipeline, a controller being provided inside the decompression chamber and a touch screen being provided at the front end, the controller and the touch screen being connected for bidirectional signals, a microwave source being provided at the top of the digestion chamber and a test tube tray being installed at the bottom, a chamber door being rotatably installed at the front end of the digestion chamber, a door groove being provided at the front end thereof engaging with the chamber door, a sealing ring groove being provided on the front end surface of the door groove, and a sealing ring being engaged in the sealing ring groove.

[0007] Furthermore, test tube slots are distributed in a circular array on the upper end surface of the test tube tray, and the test tube slots are plugged into the bottom of the test tube. A sleeve extending vertically upward is installed on the outside of each test tube slot, and a fiber optic Bragg grating temperature sensor is installed on the sleeve. The fiber optic Bragg grating temperature sensor can measure the temperature changes at various positions of the test tube.

[0008] Furthermore, a plurality of annular semiconductor refrigeration fins are distributed at equal distances from top to bottom on the circumferential outer wall of the sleeve, and thermal insulation rings are provided between the connected semiconductor refrigeration fins.

[0009] Furthermore, a card slot is provided at the bottom of the test tube tray, and the card slot is engaged with the protrusion at the bottom of the digestion chamber. Contact terminals are provided in the card slot and the protrusion. After the engagement, the contact terminals of the two are electrically connected to realize the electrical connection between the semiconductor refrigeration plate and the fiber optic Bragg grating temperature sensor and the controller.

[0010] Furthermore, an annular chamber is provided around the bottom of the decompression chamber, and a plurality of through holes are evenly distributed on the bottom of the annular chamber for communicating with the digestion chamber. The annular chamber is connected to the air inlet end of the vacuum negative pressure pump through a pipeline.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] 1. The utility model reduces the boiling point of the substance through the uniform heating and negative pressure environment provided by the microwave source, so that the digestion process can be carried out at a lower temperature, thereby improving the digestion efficiency of animal samples when determining organic selenium in animals by atomic fluorescence spectrometry.

[0013] 2. The fiber grating temperature sensor in the present invention is installed on the outside of the test tube slot through a sleeve, which can monitor the temperature changes of the sample at various positions in the test tube in real time to ensure the accuracy of temperature control during the digestion process.

[0014] 3. In the present invention, the semiconductor cooling sheets are evenly distributed on the sleeve and isolated by the thermal insulation ring. The test tube can be heated or cooled according to the temperature information fed back by the fiber grating temperature sensor to adjust the temperature during the digestion process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is an appearance diagram of a microwave digestion device for determining organic selenium in animals using atomic fluorescence spectrometry;

[0016] Figure 2 This is a schematic diagram of the internal structure of a microwave digestion device for determining organic selenium in animals using atomic fluorescence spectrometry;

[0017] Figure 3 It is a structural diagram of the vacuum negative pressure pump and the annular chamber;

[0018] Figure 4 Schematic diagram of the structure of the sealing ring;

[0019] Figure 5 It is the structural diagram of the digestion chamber;

[0020] Figure 6 This is a structural diagram from the bottom perspective of the test tube tray;

[0021] Figure 7 This is a schematic diagram of the structure from the top perspective of the test tube tray.

[0022] In the figure: 1. Box body; 2. Decompression chamber; 3. Digestion chamber; 4. Touch screen; 5. Vacuum negative pressure pump; 6. Annular chamber; 9. Chamber door; 8. Sealing ring; 10. Test tube tray; 11. Test tube slot; 12. Test tube; 13. Sleeve; 14. Fiber Bragg grating temperature sensor; 15. Insulation ring; 16. Semiconductor cooling plate; 17. Card slot; 18. Contact terminal; 19. Microwave source. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Example 1: Please refer to Figures 1 to 7A microwave digestion device for determining organic selenium in animals by atomic fluorescence method comprises a box body 1, which comprises a decompression chamber 2 and a digestion chamber 3. The decompression chamber 2 and the digestion chamber 3 are separated by a partition. A vacuum negative pressure pump 5 is installed in the decompression chamber 2, and the air inlet end of the vacuum negative pressure pump 5 is connected with the digestion chamber 3 through a pipeline. A controller is provided inside the decompression chamber 2 and a touch screen 4 is provided at the front end. The controller and the touch screen 4 are connected for two-way signals. A microwave source 19 is provided at the top of the digestion chamber 3 and a test tube tray 10 is installed at the bottom. A chamber door 9 is rotatably installed at the front end of the digestion chamber 3. A door groove is provided at the front end of the digestion chamber 3 to be engaged with the chamber door 9. A sealing ring groove is provided on the front end surface of the door groove, and a sealing ring 8 is engaged in the sealing ring groove.

[0025] An annular chamber 6 is provided around the bottom of the decompression chamber 2 . A plurality of through holes are evenly distributed on the bottom of the annular chamber 6 for communicating with the digestion chamber 3 . The annular chamber 6 is connected to the air inlet end of the vacuum negative pressure pump 5 through a pipeline.

[0026] Working principle of this embodiment:

[0027] The animal sample to be digested is placed in a test tube on the test tube tray 10 in the digestion chamber 3. The test tube must be tightly sealed to prevent sample splashing or gas leakage during the digestion process. Close the chamber door 9, ensuring that the chamber door 9 is tightly engaged with the door groove at the front end of the digestion chamber 3. At the same time, the sealing ring 8 acts as a seal in the sealing ring groove to prevent gas leakage during the digestion process. The required digestion parameters, such as temperature, time, microwave power, and negative pressure level, are input through the touch screen 4. These parameters are transmitted to the controller for storage and subsequent control. The controller controls the microwave source 19 to start operation according to the preset parameters, emitting microwave energy to the sample in the test tube in the digestion chamber 3 for heating and digestion. At the same time, the vacuum negative pressure pump 5 is activated, extracting gas from the digestion chamber 3 through the pipeline, creating a negative pressure environment. In a negative pressure environment, the boiling point of the substance is lowered, allowing the digestion process to be carried out at a lower temperature. This not only improves digestion efficiency but also reduces the emission of harmful gases and sample loss. During the digestion process, the controller uses sensors to monitor parameters such as temperature and pressure in the digestion chamber in real time and adjusts them as needed to ensure the stability and efficiency of the digestion process. When the preset digestion time is reached, the microwave source 19 and vacuum pump 5 stop operating. At this point, the chamber door 9 can be opened to remove the digested sample for subsequent processing.

[0028] The annular chamber 6 at the bottom of the decompression chamber 2 can evenly distribute the negative pressure, ensuring that the pressure in the digestion chamber 3 is evenly reduced.

[0029] In this embodiment, the uniform heating and negative pressure environment provided by the microwave source 19 lower the boiling point of the substance, so that the digestion process can be carried out at a lower temperature, thereby improving the digestion efficiency of animal samples when determining organic selenium in animals by atomic fluorescence spectrometry.

[0030] Example 2: Please refer to Figures 1 to 7 A microwave digestion device for determining organic selenium in animals by atomic fluorescence method is different from Example 1 in that a ring array of test tube slots 11 are distributed on the upper end surface of the test tube tray 10, and the test tube slots 11 are plugged into the bottom of the test tube 12. A sleeve 13 extending vertically upward is installed on the outer side of each test tube slot 11, and a fiber grating temperature sensor 14 is installed on the sleeve 13. The fiber grating temperature sensor 14 can measure the temperature changes at various positions of the test tube 12.

[0031] A plurality of annular semiconductor cooling fins 16 are distributed at equal distances from top to bottom on the circumferential outer wall of the sleeve 13 , and a heat insulating ring 15 is provided between the connected semiconductor cooling fins 16 .

[0032] A card slot 17 is provided at the bottom of the test tube tray 10, and the card slot 17 is engaged with the protrusion at the bottom of the digestion chamber 3. Contact terminals 18 are provided in the card slot 17 and the protrusion. After the engagement, the contact terminals 18 of the two are electrically connected to realize the electrical connection between the semiconductor refrigeration plate 16 and the fiber grating temperature sensor 14 and the controller.

[0033] In this embodiment, the fiber Bragg grating temperature sensor 14 is installed on the outside of the test tube slot 11 through the sleeve 13, which can monitor the temperature changes of the sample at various positions in the test tube 12 in real time, ensuring the accuracy of temperature control during the digestion process. The semiconductor refrigeration plate 16 is evenly distributed on the sleeve 13 and isolated by the thermal insulation ring 15. The test tube 12 can be heated or cooled according to the temperature information fed back by the fiber Bragg grating temperature sensor 14 to adjust the temperature during the digestion process. The card slot 17 at the bottom of the test tube tray 10 is engaged with the protrusion at the bottom of the digestion chamber 3, and the contact terminals 18 inside the two are electrically connected to realize signal transmission and power supply between the semiconductor refrigeration plate 16 and the fiber Bragg grating temperature sensor 14 and the controller, so that the controller can accurately control the digestion process.

Claims

1. A microwave digestion device for determining organic selenium in animals by atomic fluorescence spectrometry, comprising a housing (1), characterized in that: The housing (1) comprises a decompression chamber (2) and a digestion chamber (3), wherein the decompression chamber (2) and the digestion chamber (3) are separated by a partition. A vacuum negative pressure pump (5) is installed in the decompression chamber (2), and an air inlet end of the vacuum negative pressure pump (5) is connected to the digestion chamber (3) through a pipeline. A controller is provided inside the decompression chamber (2), and a touch screen (4) is provided at the front end. The controller and the touch screen (4) are connected by two-way signals. A microwave source (19) is provided at the top of the digestion chamber (3), and a test tube tray (10) is installed at the bottom. A chamber door (9) is rotatably installed at the front end of the digestion chamber (3), and a door groove is provided at the front end of the digestion chamber (3) to be engaged with the chamber door (9). A sealing ring groove is provided on the front end surface of the door groove, and a sealing ring (8) is engaged in the sealing ring groove.

2. The microwave digestion device for determining organic selenium in animals by atomic fluorescence spectrometry according to claim 1, characterized in that: Test tube slots (11) are distributed in a circular array on the upper end surface of the test tube tray (10). The test tube slots (11) are plugged into the bottom of the test tube (12). A sleeve (13) extending vertically upward is installed on the outer side of each test tube slot (11). A fiber Bragg grating temperature sensor (14) is installed on the sleeve (13). The fiber Bragg grating temperature sensor (14) can measure the temperature change at various positions of the test tube (12).

3. The microwave digestion device for determining organic selenium in animals by atomic fluorescence spectrometry according to claim 2, characterized in that: A plurality of annular semiconductor cooling fins (16) are distributed at equal distances from top to bottom on the circumferential outer wall of the sleeve (13), and a heat insulating ring (15) is provided between the connected semiconductor cooling fins (16).

4. The microwave digestion device for determining organic selenium in animals by atomic fluorescence spectrometry according to claim 2 or 3, characterized in that: The bottom of the test tube tray (10) is provided with a card slot (17), and the card slot (17) is engaged with a protrusion at the bottom of the digestion chamber (3). Contact terminals (18) are provided in both the card slot (17) and the protrusion. After the engagement, the contact terminals (18) of the two are electrically connected to realize the electrical connection between the semiconductor refrigeration plate (16) and the fiber optic Bragg grating temperature sensor (14) and the controller.

5. The microwave digestion device for determining organic selenium in animals by atomic fluorescence spectrometry according to claim 1, characterized in that: An annular chamber (6) is provided around the bottom of the decompression chamber (2). A plurality of through holes are evenly distributed on the bottom of the annular chamber (6) for communicating with the digestion chamber (3). The annular chamber (6) is connected to the air inlet end of the vacuum negative pressure pump (5) through a pipeline.

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