Nucleic acid collection tube and high-precision nucleic acid detection device

Abstract

A nucleic acid collecting tube comprises a test tube, a tube cover and an end cover, the upper end of the test tube is open, the lower end of the test tube is closed, the tube cover is arranged at the upper end of the test tube, the test tube and the tube cover jointly form a sealed tube cavity, and a first through hole communicated with the tube cavity is formed in the top wall of the tube cover. The circle center of the first through hole deviates from the geometric center of the top wall of the tube cover, an end cover is arranged on the top wall of the tube cover and rotates relative to the tube cover, the lower end face of the end cover is attached to the upper end face of the tube cover so as to cover the first through hole, a rotating shaft of the end cover is perpendicular to the axis of the tube cover, and a cotton swab enters the test tube from the first through hole. The head of the cotton swab abuts against the tube wall of one side of the test tube, the middle of the cotton swab abuts against the inner wall of the first through hole, the angle formed by the cotton swab and the test tube is small, the splashing height of collected objects on the head of the broken cotton swab is reduced, the probability that the collected objects are splashed out of the test tube is reduced, and the high-precision nucleic acid detection device is further provided.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, in particular to a nucleic acid collection tube and a high-precision nucleic acid detection device. Background Art

[0002] As the first line of defense against imported cases, customs has an important task of improving nucleic acid testing capabilities to meet the testing requirements of incoming people and animals (such as sheep).

[0003] In the existing technology, after collecting the nucleic acid sample, the tube cap must be unscrewed and a cotton swab must be inserted into the test tube, with the head of the cotton swab against the wall of one side of the test tube and the middle of the cotton swab against the wall of the other side of the test tube. The root of the cotton swab is pressed down, and the head of the cotton swab is broken in the test tube. The tube cap is screwed on and the sample is sent for testing.

[0004] like Figure 1 As shown, when the cotton swab is bent, it rests against the inner walls of the test tube on both sides, and a large angle is formed between the cotton swab and the test tube. When the cotton swab is pressed down and broken, the collected material on the head of the broken cotton swab is likely to splash out of the test tube, causing infection to the operator. Summary of the Invention

[0005] In view of this, it is necessary to propose a nucleic acid collection tube to address the above-mentioned shortcomings.

[0006] It is also necessary to propose a high-precision nucleic acid detection device.

[0007] A nucleic acid collection tube comprises a test tube, a tube cover, and an end cap. The upper end of the test tube is open, and the lower end of the test tube is closed. The tube cover is arranged at the upper end of the test tube. The test tube and the tube cover together form a sealed tube cavity. A first through hole communicating with the tube cavity is provided on the top wall of the tube cover. The center of the first through hole deviates from the geometric center of the top wall of the tube cover. An end cap is provided on the top wall of the tube cover. The end cap rotates relative to the tube cover. The lower end surface of the end cap is in contact with the upper end surface of the tube cover to cover the first through hole. The rotation axis of the end cap is perpendicular to the axis of the tube cover.

[0008] Preferably, the rotation axis of the tube cover is parallel to the axis of the test tube.

[0009] Preferably, the inner wall of the ring wall of the tube cover is threadedly connected to the outer wall of the test tube.

[0010] Preferably, the inner wall of the annular wall of the tube cover is provided with a first friction layer, and the outer wall of the test tube is provided with a second friction layer, and the first friction layer and the second friction layer are in contact.

[0011] Preferably, the upper end surface of the tube cover and the lower end surface of the end cover are both smooth planes.

[0012] Preferably, a protrusion is provided on one side of the end cover.

[0013] Preferably, the geometric center of the top wall of the tube cover is located outside the first through hole.

[0014] A high-precision nucleic acid detection device comprises a nucleic acid collection tube.

[0015] Preferably, the high-precision nucleic acid detection device also includes a droplet generator.

[0016] Preferably, the high-precision nucleic acid detection device also includes a droplet analyzer.

[0017] Beneficial effect: The cotton swab enters the test tube from the first through hole, the head of the cotton swab presses against the wall of one side of the test tube, and the middle of the cotton swab presses against the inner wall of the first through hole. The angle formed by the cotton swab and the test tube is small, and the height of the collected material on the broken head of the cotton swab splashing is reduced, thereby reducing the probability of splashing out of the test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of a cotton swab in a broken state in a test tube in the prior art.

[0019] Figure 2 This is a schematic diagram of the broken state of the cotton swab in the test tube in this application.

[0020] Figure 3 This is an axonometric view of the nucleic acid collection tube according to the first embodiment.

[0021] Figure 4 This is a partially enlarged view of the nucleic acid collection tube according to the first embodiment.

[0022] Figure 5 This is an axonometric view of the nucleic acid collection tube according to the second embodiment.

[0023] Figure 6 This is a partially disassembled view of the nucleic acid collection tube of the second embodiment.

[0024] In the figure: test tube 10, tube cover 20, first through hole 21, end cover 30, inner cover 40, second through hole 41, cotton swab 50, first rotating base 60, second rotating base 70, protrusion 80. DETAILED DESCRIPTION

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, a brief introduction will be given below to the drawings required for use in the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] See also Figures 2 to 4The embodiment of the present invention provides a nucleic acid collection tube, comprising a test tube 10, a tube cover 20, and an end cover 30. The upper end of the test tube 10 is open, and the lower end of the test tube 10 is closed. The tube cover 20 is arranged at the upper end of the test tube 10. The test tube 10 and the tube cover 20 together form a sealed tube cavity. A first through hole 21 communicating with the tube cavity is provided on the top wall of the tube cover 20. The center of the first through hole 21 deviates from the geometric center of the top wall of the tube cover 20. An end cover 30 is provided on the top wall of the tube cover 20. The end cover 30 rotates relative to the tube cover 20. The lower end surface of the end cover 30 is in contact with the upper end surface of the tube cover 20 to cover the first through hole 21. The rotation axis of the end cover 30 is perpendicular to the axis of the tube cover 20.

[0027] Beneficial effect: The cotton swab 50 enters the test tube 10 from the first through hole 21, the head of the cotton swab 50 presses against the wall of one side of the test tube 10, and the middle part of the cotton swab 50 presses against the inner wall of the first through hole 21. The angle formed by the cotton swab 50 and the test tube 10 is small, and the height of the collected material on the head of the broken cotton swab 50 is reduced, thereby reducing the probability of splashing out of the test tube 10.

[0028] In one embodiment, the lumen is spiral-shaped. After the cotton swab 50 enters the spiral lumen and is broken, the collected material on the cotton swab 50 is not likely to splash out of the lumen.

[0029] See also Figures 2 to 4 Furthermore, the rotation axis of the tube cover 20 is parallel to the axis of the test tube 10.

[0030] See also Figures 2 to 4 Furthermore, the inner wall of the ring wall of the tube cover 20 is threadedly connected to the outer wall of the test tube 10.

[0031] See also Figure 5 and Figure 6 Furthermore, the geometric center of the top wall of the tube cover 20 is located outside the first through hole 21.

[0032] See also Figure 5 and Figure 6In one embodiment, a nucleic acid collection tube includes an inner cap 40. The outer wall of the inner cap 40 is threadedly connected to the inner wall of the test tube 10. The upper end surface of the inner cap 40 is flush with the upper end surface of the test tube 10. A second through-hole 41 is provided in the inner cap 40. The diameter of the second through-hole 41 is equal to the diameter of the first through-hole 21. When the cap 20 is rotated relative to the test tube 10 to a certain position, the first through-hole 21 and the second through-hole 41 are coaxial. The thread parameters of the cap 20 and the test tube 10, as well as the thread parameters of the inner cap 40 and the test tube 10, are designed to meet the requirements that when the lower end surface of the cap 20 and the upper end surface of the inner cap 40 are in close contact, the first through-hole 21 and the second through-hole 41 do not connect or intersect. The cap 20, inner cap 40, and the test tube 10 form a sealed lumen. Compared with the prior art, the cap 20 and the inner cap 40 have a larger contact area, resulting in a better sealing effect. The tube cover 20 and the inner cover 40 rotate relative to each other, which makes it easy for the operator to control the opening and closing of the nucleic acid collection tube with one hand, and can also shear and break the cotton swab 50 quickly.

[0033] See also Figure 3 and Figure 6 Furthermore, the inner wall of the ring wall of the tube cover 20 is provided with a first friction layer, and the outer wall of the test tube 10 is provided with a second friction layer, and the first friction layer and the second friction layer are in contact.

[0034] Due to the friction between the tube cover 20 and the test tube 10, the tube cover 20 exerts a certain pressure on the inner cover 40, thereby strengthening the sealing effect between the tube cover 20 and the inner cover 40. The tube cover 20 requires a certain pulling force to separate from the test tube 10. The tube cover 20 and the test tube 10 are easy to open and close, and there is no need to design a threaded connection structure, thereby reducing manufacturing costs.

[0035] See also Figure 5 and Figure 6 Furthermore, the upper end surface of the tube cover 20 and the lower end surface of the end cover 30 are both smooth planes.

[0036] In one embodiment, the nucleic acid collection tube includes a first rotating seat 60 and a second rotating seat 70. The first rotating seat 60 is fixed to the upper end surface of the tube cover 20. The first rotating seat 60 is cylindrical, and the axis direction of the first rotation is parallel to the upper end surface of the tube cover 20. The second rotating seat 70 is fixed to one side of the end cover 30. The second rotating seat 70 is cylindrical, and the axis direction of the second rotating seat 70 is parallel to the lower end surface of the end cover 30. The first rotating seat 60 and the second rotating seat 70 are coaxially rotatably connected.

[0037] In one embodiment, the outer diameters of the first rotating base 60 and the second rotating base 70 are equal.

[0038] In one embodiment, there are two first rotating bases 60 , and the second rotating base 70 is disposed between the two first rotating bases 60 .

[0039] In one embodiment, the thickness of the end cap 30 is equal to the outer diameter of the second rotating seat 70. Exemplarily, the end cap 30 is a plate of uniform thickness, and the planes on which the upper and lower end surfaces of the end cap 30 lie are tangent to the annular wall of the second rotating seat 70. The axes of the first rotating seat 60 and the second rotating seat 70 are both located within a plane midway between the upper and lower end surfaces of the end cap 30. The distance between the axes of the first rotating seat 60 and the second rotating seat 70 and the upper end surface of the pipe cover 20 is equal to half the thickness of the end cap 30. The connection methods of the first rotating seat 60 and the pipe cover 20, and the connection methods of the second rotating seat 70 and the end cap 30 are conventional means and will not be described in detail.

[0040] In one embodiment, a third friction layer is provided on the end surface of the second rotating seat 70, and a fourth friction layer is provided on the end surface of the first rotating seat 60. The third friction layer and the fourth friction layer are in contact, so that when the second rotating seat 70 rotates relative to the first rotating seat 60, a sliding friction resistance is formed between the first rotating seat 60 and the second rotating seat 70, thereby hindering the end cover 30 from rotating relative to the pipe cover 20 and strengthening the sealing of the end cover 30 on the pipe cover 20.

[0041] See also Figure 5 and Figure 6 Furthermore, a protrusion 80 is provided on one side of the end cover 30 .

[0042] For example, the protrusion 80 is provided on the side opposite to the end cover 30 connected to the second rotating seat 70. To facilitate installation, the end face of the end cover 30 is designed to be fan-shaped, with a flat sidewall on one side connected to the second rotating seat 70, and a protrusion 80 is provided on the side opposite to the flat sidewall to facilitate the operator to open the end cover 30.

[0043] An embodiment of the utility model provides a high-precision nucleic acid detection device, including a nucleic acid collection tube.

[0044] In one embodiment, a suction cup is provided at the bottom of the test tube 10, and the high-precision nucleic acid detection device further includes a storage disk, which is a rectangular plate, and the upper surface of the storage disk is a smooth plane.

[0045] Furthermore, the high-precision nucleic acid detection device also includes a droplet generator.

[0046] Furthermore, the high-precision nucleic acid detection device also includes a droplet analyzer.

[0047] The nucleic acid collection tube is used to collect nucleic acid specimens; the storage tray is used to place several completed nucleic acid collection tubes; the transfer box is used to load the nucleic acid collection tubes loaded on the storage tray and send them to a designated location for extraction to prepare a reaction solution; the droplet generator is used to generate 20,000 nanoliter-sized droplets from the reaction solution, each droplet containing or not containing a nucleic acid target molecule. Each droplet acts as an independent PCR reactor and then undergoes PCR amplification; the droplet analyzer is used to detect each droplet one by one, with the presence of a fluorescent signal being interpreted as "1" and the absence of a fluorescent signal being interpreted as "0". Finally, based on the Poisson distribution principle and the proportion of positive droplets, the analysis software calculates the concentration of the target molecule to be tested.

[0048] The droplet generator is specifically a QX200 droplet generator, and the droplet analyzer is specifically a QX200 droplet analyzer. The QX200 droplet analyzer and the QX200 droplet generator constitute the QX200 Droplet Digital PCR system.

[0049] The droplet generator installed in the high-precision nucleic acid detection device can achieve absolute quantification and multiple detection. Multiple detection can be understood as the simultaneous detection of multiple pathogens. The detection process is fast and highly accurate. The droplet generator installed in the high-precision nucleic acid detection device can realize the digitization and visualization of the detection results.

[0050] The modules or units in the device of the embodiment of the present utility model can be combined, divided and deleted according to actual needs.

[0051] The above disclosure is only a preferred embodiment of the present invention, and it is certainly not intended to limit the scope of the rights of the present invention. A person skilled in the art can understand that all or part of the processes of the above embodiment and equivalent changes made in accordance with the claims of the present invention are still within the scope of the present invention.

Claims

1. A nucleic acid collection tube, characterized in that: The test tube comprises a test tube, a tube cover, and an end cover. The upper end of the test tube is open, and the lower end of the test tube is closed. The tube cover is arranged at the upper end of the test tube. The test tube and the tube cover together form a sealed tube cavity. A first through hole communicating with the tube cavity is provided on the top wall of the tube cover. The center of the first through hole deviates from the geometric center of the top wall of the tube cover. An end cover is provided on the top wall of the tube cover. The end cover rotates relative to the tube cover. The lower end surface of the end cover is fitted with the upper end surface of the tube cover to cover the first through hole. The rotation axis of the end cover is perpendicular to the axis of the tube cover.

2. The nucleic acid collection tube according to claim 1, wherein: The rotation axis of the tube cover is parallel to the axis of the test tube.

3. The nucleic acid collection tube according to claim 2, wherein: The inner wall of the ring wall of the tube cover is threadedly connected to the outer wall of the test tube.

4. The nucleic acid collection tube according to claim 2, wherein: The inner wall of the annular wall of the tube cover is provided with a first friction layer, and the outer wall of the test tube is provided with a second friction layer, and the first friction layer is in contact with the second friction layer.

5. The nucleic acid collection tube according to claim 1, wherein: The upper end surface of the tube cover and the lower end surface of the end cover are both smooth planes.

6. The nucleic acid collection tube according to claim 1, wherein: A protrusion is provided on one side of the end cover.

7. The nucleic acid collection tube according to claim 1, wherein: The geometric center of the top wall of the tube cover is located outside the first through hole.

8. A high-precision nucleic acid detection device, characterized by: Comprising the nucleic acid collection tube according to claim 1.

9. The high-precision nucleic acid detection device according to claim 8, characterized in that: The high-precision nucleic acid detection device also includes a droplet generator.

10. The high-precision nucleic acid detection device according to claim 9, characterized in that: The high-precision nucleic acid detection device also includes a droplet analyzer.

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