Horizontal table sleep deprivation device for rats and mice

Abstract

The utility model belongs to the technical field of biological medicine experiment devices, and particularly relates to a horizontal table sleep deprivation device for rats and mice. Comprising a sleep deprivation box and an environment contrast box, and each of the sleep deprivation box and the environment contrast box comprises a water containing box body filled with water; the supporting columns are arranged at the bottom of the water containing tank body; annular positioning pieces are arranged on the outer sides of the supporting columns. The first horizontal table is provided with a first mesh hole and a third mesh hole, and the first mesh hole penetrates through the supporting column and is fixed at the position of the annular positioning piece; the cage cover feeding trough is arranged at the top of the water containing tank body and is detachably connected with the water containing tank body; in the environment contrast box, a second horizontal table is arranged at the top end of the supporting column, and second meshes are formed in the second horizontal table. Sleep deprivation experiments can be carried out on multiple animals at the same time at a time, test switching of rats and mice can be achieved, and the deprivation device is convenient to clean.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedical experimental devices, in particular to a water platform sleep deprivation device for mice and rats. Background Art

[0002] Sleep is an essential physiological process that regulates energy metabolism, endocrine function, cardiovascular function, and the immune system, and is crucial for maintaining healthy homeostasis. With the increasing pace of life and the emergence of unhealthy lifestyles, such as chronic sleep deprivation, cross-time zone travel, and shift work, sleep deprivation is becoming increasingly prominent. According to statistics, as many as one-third of adults worldwide experience insomnia, and epidemiological studies have shown that the prevalence of sleep disorders continues to increase. Epidemiological studies have confirmed that sleep disorders can lead to brain dysfunction, decreased immunity, irritability, and the development of conditions such as obesity, metabolic syndrome, and type 2 diabetes. Studying the effects of sleep deprivation on human physiological functions is of great practical significance. However, research on sleep deprivation and its associated pathological mechanisms is insufficient. Therefore, developing a sleep deprivation device to provide a scientifically reliable sleep deprivation experimental animal model is crucial for studying the pathological mechanisms of sleep disorders.

[0003] Sleep deprivation is an important method for studying the functions and mechanisms of sleep. It refers to a state in which the body partially or completely loses its normal sleep intake due to various reasons. Depending on the amount of sleep lost, sleep deprivation can be categorized as complete, partial, or selective. Depending on the severity of the onset of sleep deprivation, it can be categorized as acute or chronic. The quality of sleep deprivation conditions directly impacts the reliability and accuracy of the experiment and has a significant impact on the experimental results. Currently, the platform water environment method is one of the most commonly used methods for establishing sleep deprivation models. This method exploits the rat's fear of water and inability to fall asleep in water. A platform is placed in a water tank, and the rat / mice are placed on it. Because the platform's diameter is small (≤ 6.5 cm / 3.5 cm), once the rat / mice enters a sleep state, they rhythmically lower their head due to a decrease in muscle tone. This causes the rat / mice to fall into the water below the platform, awakening them. They then climb back onto the platform and continue to maintain their original posture, remaining awake and standing, thus achieving the desired effect of sleep deprivation. This method is mainly used for rapid eye movement (REM) sleep deprivation of experimental animals. It is simple and easy to perform, does not require complex and expensive equipment, and can be carried out in laboratories under different conditions. Therefore, it has a wide range of applications.

[0004] However, the current structure of the sleep deprivation chamber used in the laboratory is not rationally designed, and has the following obvious deficiencies: 1) Only one animal can be deprived during the experiment, and the number of samples that can be processed at one time is small. Single-animal experiments are prone to stress reactions caused by group isolation; 2) The cage cover designed on the top of the chamber does not completely cover the animal, making it easy for the animal to escape from the sleep deprivation chamber, or it completely covers but affects the air quality inside the chamber, interfering with the experimental results; 3) The feeding trough is set on the side of the chamber and suspended directly above the animal water platform. When the animal eats, it needs to swim to the feeding trough, or it is difficult to maintain balance during the feeding process. During the experiment, the experimental animals cannot eat and drink freely, which is not conducive to scientific experiments; 4) The water tank is heavy, and the platform is fixed to the bottom of the trough and cannot be moved or replaced, which is not conducive to cleaning.

[0005] Therefore, it is necessary to provide a sleep deprivation device that can perform multiple animal experiments and has rigorous sleep deprivation and is easy to clean. Utility Model Content

[0006] The utility model provides a horizontal platform sleep deprivation device for mice and rats in order to solve the problems that a sleep deprivation box used in a laboratory cannot be used for multiple animal experiments and the deprivation box is difficult to clean.

[0007] The utility model adopts the following technical solution: a water platform sleep deprivation device for mice and rats, comprising a sleep deprivation box and an environmental control box, wherein the sleep deprivation box and the environmental control box both comprise:

[0008] a water storage box filled with water;

[0009] A plurality of pillars are arranged at the bottom of the water tank; an annular positioning piece is arranged on the outside of the pillars;

[0010] A first horizontal platform, wherein a first mesh and a third mesh are provided on the first horizontal platform, wherein the first mesh passes through the pillar and is fixed at the annular positioning piece;

[0011] A cage-covered feeding trough, which is arranged on the top of the water tank and is detachably connected to the water tank;

[0012] In the environmental control box, a second horizontal platform is provided at the top of the support column, and a second mesh is provided on the second horizontal platform.

[0013] Furthermore, the support comprises a column and a standing platform, wherein the standing platform is a horizontal platform and is arranged on the top of the column; the annular positioning piece is sleeved on the outside of the column.

[0014] Furthermore, a plurality of slots are provided at the bottom of the water storage box; the upright posts are detachably connected to the slots, and the upright posts correspond to the slots one by one.

[0015] Furthermore, the card slots are divided into large and small card slots, which are arranged at intervals, and the pillars are divided into large and small pillars, which correspond to the large and small card slots respectively.

[0016] Furthermore, the cage cover feeding trough is a continuous V-shaped structure, and the mesh of the cage cover feeding trough corresponds to the position of the standing platform up and down, which is convenient for feeding; there are triangular baffles on both sides of the V-shape of the cage cover feeding trough.

[0017] Furthermore, lock buckles are installed on both sides of the water storage box, and lock slots are provided at both ends of the cage cover feeding trough. The lock slots and lock buckles connect the cage cover feeding trough with the water storage box.

[0018] Furthermore, a water bottle is fixed on the mesh of the cage cover feeding trough.

[0019] Furthermore, the column is provided with a scale, and the water level line in the water tank is 1 to 1.5 cm below the standing platform.

[0020] Furthermore, a drain outlet is provided on the bottom side wall of the water storage box.

[0021] Furthermore, the water tank is made of transparent acrylic material, and the support, cage cover feeding trough, first horizontal platform and second horizontal platform are all made of stainless steel.

[0022] Compared with the prior art, the beneficial effects of the present invention are:

[0023] 1. The utility model can conduct sleep deprivation experiments on multiple animals at the same time, realize parallel experiments between groups, save scientific research time, and the experimental results are stable and repeatable, thereby improving scientific research efficiency.

[0024] 2. The pillars and grooves of the present invention are detachably connected, and the number and size of the pillars can be replaced according to actual needs, which is convenient for experiments on large and small mice; after the experiment is completed, the pillars can be removed for easy cleaning.

[0025] 3. The water tank and the cage cover feeding trough are connected by a snap-on connection, and the V-shaped cage cover feeding trough has triangular baffles on both sides, which is convenient for placing experimental materials and preventing animals from escaping from the sleep deprivation box; the mesh of the cage cover feeding trough corresponds to the position of the standing platform, making it more convenient for sleep-deprived animals to eat and drink water.

[0026] 4. The environmental control chamber is as similar to the sleep deprivation chamber as possible, ensuring the scientific nature of the experiment. In addition, the environmental control chamber adds a second water platform, which effectively reduces the interference of other factors such as water contact in animal sleep deprivation experiments on the experimental results, making the experimental data more meaningful and more valuable for reference. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1This is a schematic structural diagram of a sleep deprivation box of the utility model sleep deprivation device;

[0028] Figure 2 This is a schematic structural diagram of the environmental control box of the sleep deprivation device of the utility model;

[0029] Figure 3 This is a top view of the water storage box of the utility model;

[0030] Figure 4 This is a schematic diagram of the structure of the first water platform of the utility model;

[0031] Figure 5 This is a schematic diagram of the structure of the second horizontal platform of the utility model;

[0032] Figure 6 This is a schematic diagram of the pillar structure of the utility model;

[0033] Figure 7 This is a schematic diagram of the structure of the cage cover feeding trough of the utility model;

[0034] In the figure: 1. Water container; 2. Support column; 3. First horizontal platform; 4. Cage cover feeding trough; 5. Second horizontal platform; 6. Annular positioning piece; 7. Slot; 8. Column; 9. Standing platform; 10. First mesh hole; 11. Second mesh hole; 12. Third mesh hole; 13. Drainage outlet; 14. Water feeding bottle; 15. Triangular baffle. DETAILED DESCRIPTION

[0035] The following will be combined with the accompanying drawings in the embodiments of the present invention to further clearly and completely describe the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Utilizing the technical solutions described in the present invention, or those skilled in the art who are inspired by the technical solutions of the present invention and design similar technical solutions to achieve the above-mentioned technical effects, all fall within the scope of protection of the present invention.

[0036] This embodiment provides a horizontal platform sleep deprivation device for mice and rats. Figure 1-Figure 2 , Figure 4-Figure 5 As shown, it includes: a sleep deprivation box and an environmental control box, and both the sleep deprivation box and the environmental control box include:

[0037] A water storage tank 1 is filled with water;

[0038] A plurality of pillars 2 are provided at the bottom of the water tank 1; an annular positioning piece 6 is provided on the outside of the pillars 2;

[0039] The first horizontal platform 3 is provided with a first mesh 10 and a third mesh 12. The first mesh 10 passes through the support 2 and is fixed at the annular positioning piece 6 to position the first horizontal platform 3;

[0040] The cage cover feeding trough 4 is arranged on the top of the water tank body 1 and is detachably connected to the water tank body 1;

[0041] In the environmental control box, a second horizontal platform 5 is provided at the top of the support 2 , and a second mesh 11 is provided on the second horizontal platform 5 .

[0042] During the control experiment, mice were placed on the second horizontal platform 5 of the environmental control box. The pillars 2 at the bottom served as a support, allowing the mice to move freely and eat on the second horizontal platform 5 without falling into the water. The environmental control box is as similar to the sleep deprivation box as possible, reducing experimental errors.

[0043] like Figure 6 As shown, support column 2 includes a column 8 and a standing platform 9. Standing platform 9 is a horizontal platform, located at the top of column 8; an annular positioning piece 6 is positioned outside column 8. Column 8 is graduated, and the water level in water tank 1 is 1 to 1.5 cm below standing platform 9. One experimental mouse stands on each standing platform 9. During experiments, water is added to water tank 1 until the water level is 1 to 1.5 cm below the standing platform 9. This allows the mouse to wake up when it is exhausted and return to the standing platform 9.

[0044] like Figure 3 As shown, a plurality of slots 7 are provided at the bottom of the water tank 1; upright posts 8 are detachably connected to the slots 7, and the upright posts 8 correspond to the slots 7 one by one.

[0045] In some embodiments, the slot 7 is a raised ring, the inner diameter of which is the same as the outer diameter of the pillar 8, and the pillar 8 can be inserted into the annular slot 7 for fixation. After the experiment is completed, the pillar 8 can be separated from the slot 7 by gently pulling it.

[0046] In some embodiments, the card slot 7 is divided into large and small card slots, and the large and small card slots are arranged at intervals. The pillar 2 is divided into large and small pillars, and is respectively arranged corresponding to the large and small card slots.

[0047] The slots 7 match the size of the columns. For rat experiments, a large column is used. This column is aligned with the large slot and placed at the bottom of the water tank 1. A first horizontal platform 3 with a first mesh 10 aperture that matches the large column is then mounted on multiple large columns. The first horizontal platform 3 is secured at the annular positioning piece 6 to further enhance the stability of the column 2 and the water tank 1. For mouse experiments, a small column is used. This column is aligned with the small slot and placed at the bottom of the water tank 1. A first horizontal platform 3 with a first mesh 10 aperture that matches the small column is then mounted on multiple small columns. This way, only one water tank 1 is required, and switching between experiments with large and small mice can be accomplished by replacing the columns 2 and the first horizontal platform 3.

[0048] The sizes of the first horizontal platform 3 and the second horizontal platform 5 match the size of the water tank 1, which can prevent the multiple standing platforms 9 from shaking when placed in water and affecting the experimental results.

[0049] like Figure 7 As shown, the cage cover feeding trough 4 is a continuous V-shaped structure, and a water bottle 14 is fixed to the mesh of the cage cover feeding trough 4. The mesh of the cage cover feeding trough 4 corresponds to the position of the standing platform 9, which is convenient for feeding; triangular baffles 15 are provided on both sides of the V-shaped cage cover feeding trough 4 to prevent animal feed from sliding from the sides and also to prevent animals from escaping from the deprivation box.

[0050] In some embodiments, locks are installed on both sides of the water tank body 1, and lock slots are provided at both ends of the cage cover feeding trough 4. One end of the lock is buckled on the side handle 1 of the water tank body 1, and the other end is connected to the lock slot of the cage cover feeding trough 4 through a snap-fit ​​device, thereby snap-fitting the cage cover feeding trough 4 to the water tank body 1.

[0051] In some embodiments, the apertures of the third mesh 12 and the second mesh 11 are both smaller than the aperture of the first mesh 10 , so as to facilitate water injection into the bottom of the water storage tank 1 .

[0052] In some embodiments, the bottom sidewall of the water tank 1 is provided with a drain port 13. The experimental box is heavy when filled with water and is difficult to move. The drain port 13 can be used to drain the experimental water first, reducing the weight and making it easier to clean.

[0053] In some embodiments, the water tank 1 is made of transparent acrylic, while the support 2, cage cover and feeding trough 4, first and second horizontal platforms 3, and 5 are all made of stainless steel. The transparent housing does not obstruct light entry or disrupt circadian rhythms, and is lightweight, compact, and easy to use and move. The support 2, cage cover and feeding trough 4, first and second horizontal platforms 3 and 5 are made of stainless steel, ensuring durability and resistance to gnawing by rats and mice.

[0054] In summary, the utility model provides a sleep deprivation device for rats and mice on a water platform that can conduct sleep deprivation experiments on multiple animals at the same time, which has important scientific research value. The utility model has a simple structure, a reasonable design, and is easy to use. It can conduct sleep deprivation experiments on rats and mice respectively. By designing a control box, the scientific requirements of animal experiments can be met. Sleep deprivation experiments can be conducted on multiple animals in parallel, saving scientific research time, making the experimental results parallel and stable, and highly repeatable, thereby improving scientific research efficiency. The device is also detachable, easy to clean, highly operable, and can be stacked, with high space utilization. Therefore, the utility model provides a sleep deprivation model device that can meet scientific rigor, ensures the efficient completion of sleep deprivation animal experiments, and lays a foundation for studying the pathological mechanisms of sleep disorders and the effects of sleep deprivation on human physiological functions.

[0055] In the description of the present invention, it should be understood that the terms "inside", "outside", "large", "small", etc., indicating positions or size relationships, are based on the positions or size relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0056] In this utility model, unless otherwise specified or limited, the terms "connection" and "fixed" should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration, unless otherwise specified. Those skilled in the art will understand the specific meanings of these terms in this invention based on specific circumstances.

[0057] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Thus, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, "several" means at least one, for example, two, three, etc., unless otherwise specifically defined.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A horizontal platform sleep deprivation device for rats and mice, characterized in that: It comprises a sleep deprivation box and an environmental control box, and the sleep deprivation box and the environmental control box both comprise: A water storage box (1), wherein the water storage box (1) is filled with water; A plurality of pillars (2), wherein the pillars (2) are arranged at the bottom of the water tank (1); an annular positioning piece (6) is arranged on the outside of the pillars (2); A first horizontal platform (3), wherein a first mesh (10) and a third mesh (12) are provided on the first horizontal platform (3), and the first mesh (10) passes through the pillar (2) and is fixed at the annular positioning piece (6); A cage-covered feeding trough (4), wherein the cage-covered feeding trough (4) is arranged on the top of the water storage box (1) and is detachably connected to the water storage box (1); In the environmental control box, a second horizontal platform (5) is provided at the top of the support (2), and a second mesh (11) is provided on the second horizontal platform (5).

2. The horizontal platform sleep deprivation device for mice and rats according to claim 1, characterized in that: The support column (2) comprises a column (8) and a standing platform (9), wherein the standing platform (9) is a horizontal platform and is arranged on the top of the column (8); the annular positioning piece (6) is sleeved on the outside of the column (8).

3. The horizontal platform sleep deprivation device for mice and rats according to claim 2, characterized in that: The bottom of the water storage box (1) is provided with a plurality of slots (7); the upright posts (8) are detachably connected to the slots (7), and the upright posts (8) correspond to the slots (7) in a one-to-one manner.

4. The horizontal platform sleep deprivation device for mice and rats according to claim 3, characterized in that: The card slots (7) are divided into large and small card slots, which are arranged at intervals, and the pillars (2) are divided into large and small pillars, which correspond to the large and small card slots, respectively.

5. The horizontal platform sleep deprivation device for mice and rats according to claim 2, characterized in that: The cage cover feeding trough (4) is a continuous V-shaped structure, and the mesh of the cage cover feeding trough (4) corresponds to the position of the standing platform (9) up and down, which is convenient for feeding; triangular baffles (15) are provided on both sides of the V-shape of the cage cover feeding trough (4).

6. The horizontal platform sleep deprivation device for mice and rats according to claim 1, characterized in that: Lock buckles are installed on both sides of the water storage box (1), and locking grooves are provided at both ends of the cage cover feeding trough (4). The locking grooves and the lock buckles connect the cage cover feeding trough (4) and the water storage box (1) by snapping.

7. The horizontal platform sleep deprivation device for mice and rats according to claim 1, characterized in that: A water bottle (14) is fixed on the mesh of the cage cover feeding trough (4).

8. The horizontal platform sleep deprivation device for rats and mice according to claim 4, characterized in that: The upright column (8) is provided with a scale, and the water level in the water tank (1) is 1 to 1.5 cm below the standing platform (9).

9. The horizontal platform sleep deprivation device for rats and mice according to claim 4, characterized in that: A drainage outlet (13) is provided on the bottom side wall of the water storage box (1).

10. The horizontal platform sleep deprivation device for rats and mice according to claim 4, characterized in that: The water tank (1) is made of transparent acrylic material, and the support (2), the cage cover feeding trough (4), the first water platform (3) and the second water platform (5) are all made of stainless steel.

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