Wheat leaf sample DNA extraction device

Abstract

The utility model discloses a wheat leaf sample DNA extraction device, relates to DNA extraction technical field, the device comprises a base, a grinding tank, a water tank and a grinding assembly, the grinding tank is arranged in the base, the water tank is installed on one side of the top of the base, the grinding assembly comprises an electric push rod, the electric push rod is installed on one side of the top of the base, and the electric push rod is connected with the grinding assembly. The output end of the electric push rod is fixedly connected with a connecting plate, a motor is installed on the top of one side of the connecting plate, the output end of the motor is fixedly connected with a connecting rod, grinding rollers are installed on the two sides of the bottom of the connecting rod, and a cleaning assembly used for cleaning the grinding tank is arranged in the water tank. According to the wheat leaf sample grinding device, the position of the grinding roller can be adjusted according to the size and the requirement of wheat leaf samples through the grinding assembly, the grinding tank can be rapidly cleaned through the brush plate and the cleaning assembly, cross infection of the samples is avoided, and sanitation of equipment and the purity of the samples are guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA extraction, in particular to a device for extracting DNA from wheat leaf samples. Background Art

[0002] A DNA extraction device is a device used to extract DNA (deoxyribonucleic acid) from biological samples (such as plants, animals, microorganisms, etc.). DNA extraction devices are widely used in molecular biology, genomics, forensic medicine, agricultural science and other fields. A wheat leaf sample DNA extraction device is a device specifically used to extract DNA from wheat leaves. It improves the efficiency and quality of DNA extraction through automation or semi-automation.

[0003] For example, the cucumber DNA extraction device described in patent document CN217868805 U describes a method for extracting DNA from cucumbers. The device removes the top cover, adds cucumber tissue and liquid nitrogen to a mortar, then replaces the top cover and lowers the pestle into the mortar. The drive motor is activated to rotate the connecting rod, which in turn moves the disc, causing the mortar to rock relative to the pestle, thereby grinding the contents and extracting the DNA. The top cover can optionally be provided with ventilation holes to facilitate ventilation. Alternatively, a switch can be installed on the periphery of the base to control the start and stop of the drive motor.

[0004] In the above case, when using a grinding jar to grind the sample, it is difficult to make corresponding adjustments according to the size of the sample, which is not convenient for grinding larger samples. At the same time, after the crude extraction is completed, it is difficult to clean the grinding jar, which is likely to cause cross-infection of the sample and is not conducive to the repeated use of the sample. Utility Model Content

[0005] The purpose of the utility model is to provide a device for extracting DNA from wheat leaf samples to solve the problems in the background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] Wheat leaf sample DNA extraction device, including:

[0008] base;

[0009] A grinding jar, the grinding jar being arranged inside the base;

[0010] a water tank mounted on one side of the top of the base;

[0011] It also includes a grinding assembly, which includes an electric push rod, which is installed on one side of the top of the base, and the output end of the electric push rod is fixedly connected to a connecting plate, a motor is installed on the top of one side of the connecting plate, and the output end of the motor is fixedly connected to a connecting rod, which passes through the grinding tank, and grinding rollers are installed on both sides of the bottom of the connecting rod. A cleaning assembly for cleaning the grinding tank is provided in the water tank.

[0012] On the basis of the above technical solutions, the present invention also provides the following optional technical solutions:

[0013] In an optional scheme: the cleaning component includes a water pump, which is installed on the top of the water tank, the suction end of the water pump is passed through the inside of the water tank, the discharge end of the water pump is connected to a water pipe, the discharge end of the water pipe is fixedly connected to the grinding tank, a first solenoid valve is provided on the water pipe, the bottom of the connecting rod is fixedly connected to a brush plate, the bottom of the brush plate is provided with a brush, the bottom of the grinding tank is connected to a water outlet, and a third solenoid valve is installed at the water outlet.

[0014] In an optional solution, a discharge assembly for discharging the ground items is provided at the bottom of the grinding jar.

[0015] In an optional solution: the discharging assembly includes an electric telescopic rod, which is installed on the side of the grinding jar away from the electric push rod, and the output end of the electric telescopic rod is fixedly connected to an L-shaped plate, and one side of the L-shaped plate is fixedly connected to a sliding block, and sliding ears are provided on both sides of the sliding block. A discharging port is opened at the bottom of the grinding jar, and the sliding ears are slidably connected to the discharging port, and a discharging hopper is installed at the bottom of the discharging port.

[0016] In an optional solution: a groove corresponding to the position of the sliding block and the discharge hopper is formed on a side of the base away from the electric push rod.

[0017] In an optional solution, a liquid inlet is provided on the top of the grinding jar on a side away from the water pipe, and a second solenoid valve is provided on the liquid inlet.

[0018] In an optional solution, a feed port is provided on the top of the grinding jar away from the electric push rod.

[0019] In an optional solution: the brush plate is U-shaped.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] The utility model can adjust the position of the grinding roller according to the size and demand of the wheat leaf sample through the grinding component, thereby ensuring a better grinding effect and reducing manual intervention. The grinding tank can be quickly cleaned through the brush plate and cleaning component to avoid cross infection of samples, thereby ensuring the hygiene of the equipment and the purity of the sample. The ground sample can also be quickly discharged. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural diagram of the present utility model.

[0023] Figure 2 It is a schematic diagram of the cutaway structure of the present utility model.

[0024] Figure 3 It is a schematic diagram of the partial structure of the cleaning component in the utility model.

[0025] Among them: 100, base; 200, grinding jar; 300, water tank; 401, electric push rod; 402, connecting plate; 403, motor; 404, connecting rod; 405, grinding roller; 501, water pump; 502, water pipe; 503, first solenoid valve; 504, brush plate; 601, electric telescopic rod; 602, L-shaped plate; 603, sliding block; 604, sliding ear; 605, discharge port; 606, discharge hopper; 701, groove; 702, liquid inlet; 703, feed port. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings and embodiments.

[0027] In one embodiment, Figure 1-Figure 3As shown, the wheat leaf sample DNA extraction device includes: a base 100, a grinding tank 200, a water tank 300 and a grinding assembly, wherein the grinding tank 200 is arranged inside the base 100, the water tank 300 is installed on one side of the top of the base 100, and the grinding assembly includes an electric push rod 401, which is installed on one side of the top of the base 100. The output end of the electric push rod 401 is fixedly connected to a connecting plate 402, and a motor 403 is installed on the top of one side of the connecting plate 402. The output end of the motor 403 is fixedly connected to a connecting rod 404, and the connecting rod 404 passes through The grinding jar 200 has grinding rollers 405 installed on both sides of the bottom of the connecting rod 404, and the water tank 300 is provided with a cleaning component for cleaning the grinding jar 200; by starting the motor 403, the connecting rod 404 is driven to rotate, and the grinding roller 405 is driven to rotate, so that the grinding roller 405 grinds the objects in the grinding jar 200, and at the same time, the electric push rod 401 can be started to drive the connecting plate 402 and the motor 403 to move up and down, thereby driving the connecting rod 404 and the grinding roller 405 to move up and down, so that the position of the grinding roller 405 can be adjusted to facilitate grinding objects of different sizes.

[0028] In one embodiment, Figure 1 and Figure 2 As shown, the cleaning component includes a water pump 501, which is installed on the top of the water tank 300, and the suction end of the water pump 501 is passed through the inside of the water tank 300, and the discharge end of the water pump 501 is connected to a water pipe 502, and the discharge end of the water pipe 502 is fixedly connected to the grinding tank 200, and a first solenoid valve 503 is provided on the water pipe 502, and the bottom of the connecting rod 404 is fixedly connected to a brush plate 504, and the bottom of the brush plate 504 is provided with a brush, and the bottom of the grinding tank 200 is connected to a water outlet, and a third solenoid is installed at the water outlet; by starting the water pump 501, water is sucked into the interior of the grinding tank 200, and through cooperation with the grinding component, the brush plate 504 cleans the inner wall of the grinding tank 200, and then the liquid is discharged through the water outlet.

[0029] In one embodiment, Figure 1 and Figure 2As shown, the discharging assembly includes an electric telescopic rod 601, which is installed on the side of the grinding jar 200 away from the electric push rod 401, and the output end of the electric telescopic rod 601 is fixedly connected to an L-shaped plate 602, and one side of the L-shaped plate 602 is fixedly connected to a sliding block 603, and sliding ears 604 are provided on both sides of the sliding block 603. A discharge port 605 is opened at the bottom of the grinding jar 200, and the sliding ears 604 are slidably connected to the discharge port 605, and a discharge hopper 606 is installed at the bottom of the discharge port 605; by starting the electric telescopic rod 601, the L-shaped plate 602 is driven to move, thereby driving the sliding ears 604 on the sliding block 603 to slide inside the discharge port 605, so that the ground objects can be easily sent out of the grinding jar 200 by the discharge hopper 606.

[0030] In one embodiment, Figure 1 As shown, a groove 701 corresponding to the position of the sliding block 603 and the discharge hopper 606 is provided on the side of the base 100 away from the electric push rod 401; the groove 701 facilitates the sliding of the sliding block 603.

[0031] In one embodiment, Figure 1 As shown, a liquid inlet 702 is provided on the top of the grinding jar 200 away from the water pipe 502 , and a second solenoid valve is provided on the liquid inlet 702 ; by opening the second solenoid valve, liquid nitrogen is introduced into the grinding jar 200 through the liquid inlet 702 .

[0032] In one embodiment, Figure 2 As shown, a feed port 703 is provided on the top of the grinding jar 200 away from the electric push rod 401 ; wheat leaves are placed into the grinding jar 200 through the feed port 703 for rough extraction.

[0033] In one embodiment, Figure 3 As shown, the brush plate 504 is U-shaped; the inner wall of the grinding jar 200 is cleaned by the brush plate 504, and the ground objects can also be brushed to the discharge port 605.

[0034] The above embodiment discloses a wheat leaf sample DNA extraction device, wherein the wheat leaf is first placed into the grinding jar 200 through the feed port 703, then the second solenoid valve is opened, and liquid nitrogen is placed into the grinding jar 200 through the liquid inlet 702 to perform a cold treatment on the wheat leaf, making the wheat leaf easier to grind. Then, the height of the grinding roller 405 is adjusted according to the size of the wheat leaf, and the electric push rod 401 is started to drive the connecting plate 402 and the motor 403 to move up and down, thereby driving the connecting rod 404 and the grinding roller 405 to move up and down, thereby adjusting the upper and lower positions of the grinding roller 405, and then the motor 403 is turned on to drive the connecting rod 40 4 rotates, and drives the grinding roller 405 to rotate, so that the grinding roller 405 grinds the objects in the grinding jar 200. After grinding, the electric telescopic rod 601 is started to drive the L-shaped plate 602 to move, thereby driving the sliding ear 604 on the sliding block 603 to slide inside the discharge port 605, so that the ground objects can be easily sent out of the grinding jar 200 through the discharge hopper 606. Then, the grinding jar 200 is cleaned, and water is sucked into the interior of the grinding jar 200 by starting the water pump 501. Through cooperation with the grinding assembly, the brush plate 504 cleans the inner wall of the grinding jar 200, and then the liquid is discharged through the water outlet.

[0035] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. Wheat leaf sample DNA extraction device, including: Base (100); A grinding jar (200), wherein the grinding jar (200) is arranged inside the base (100); A water tank (300), the water tank (300) being installed on one side of the top of the base (100); The invention is characterized in that it further comprises a grinding assembly, wherein the grinding assembly comprises an electric push rod (401), the electric push rod (401) is mounted on one side of the top of the base (100), the output end of the electric push rod (401) is fixedly connected to a connecting plate (402), a motor (403) is mounted on the top of one side of the connecting plate (402), the output end of the motor (403) is fixedly connected to a connecting rod (404), the connecting rod (404) passes through the grinding jar (200), grinding rollers (405) are mounted on both sides of the bottom of the connecting rod (404), and a cleaning assembly for cleaning the grinding jar (200) is provided in the water tank (300).

2. The wheat leaf sample DNA extraction device according to claim 1, characterized in that: The cleaning assembly comprises a water pump (501), the water pump (501) is installed on the top of the water tank (300), the suction end of the water pump (501) is arranged inside the water tank (300), the discharge end of the water pump (501) is connected to a water pipe (502), the discharge end of the water pipe (502) is fixedly connected to the grinding tank (200), a first solenoid valve (503) is provided on the water pipe (502), the bottom of the connecting rod (404) is fixedly connected to a brush plate (504), the bottom of the brush plate (504) is provided with a brush, the bottom of the grinding tank (200) is connected to a water outlet, and a third solenoid valve is installed at the water outlet.

3. The wheat leaf sample DNA extraction device according to claim 1, characterized in that: The bottom of the grinding jar (200) is provided with a discharge assembly for discharging the ground objects.

4. The wheat leaf sample DNA extraction device according to claim 3, characterized in that: The discharging assembly comprises an electric telescopic rod (601), the electric telescopic rod (601) being mounted on a side of the grinding jar (200) away from the electric push rod (401), an output end of the electric telescopic rod (601) being fixedly connected to an L-shaped plate (602), one side of the L-shaped plate (602) being fixedly connected to a sliding block (603), sliding ears (604) being provided on both sides of the sliding block (603), a discharging port (605) being provided at the bottom of the grinding jar (200), the sliding ears (604) being slidably connected to the inside of the discharging port (605), and a discharging hopper (606) being mounted at the bottom of the discharging port (605).

5. The wheat leaf sample DNA extraction device according to claim 4, characterized in that: A groove (701) corresponding to the position of the sliding block (603) and the discharge hopper (606) is provided on a side of the base (100) away from the electric push rod (401).

6. The wheat leaf sample DNA extraction device according to claim 2, characterized in that: A liquid inlet (702) is provided on the top of the grinding jar (200) on a side away from the water pipe (502), and a second solenoid valve is provided on the liquid inlet (702).

7. The wheat leaf sample DNA extraction device according to claim 1, characterized in that: A feed port (703) is provided on the top of the grinding jar (200) on a side away from the electric push rod (401).

8. The wheat leaf sample DNA extraction device according to claim 2, characterized in that: The brush plate (504) is U-shaped.

Images