Gel hole alignment structure for agarose gel electrophoresis sample application

Abstract

The utility model discloses a glue hole alignment structure for agarose gel electrophoresis sample application, which comprises a placing box, the upper side of the left end of the placing box is fixedly connected with two vertical plates, a rotating rod is rotatably connected between the two vertical plates, the outer side of the rotating rod is fixedly connected with a rotating block, the lower side of the rotating block is fixedly connected with a rotating plate, and the rotating plate is fixedly connected with the left end of the placing box. A plurality of alignment units are arranged on the upper side of the rotating plate, each alignment unit comprises a round hole, the round holes are formed in the upper side of the rotating plate, sliding holes are formed in the upper side of the rotating plate and located in the left side and the right side of the round holes, and sliding blocks are slidably connected to the inner sides of the sliding holes in the left side and the right side. By arranging a rotating plate, a round hole, a sliding hole, a sliding block, a clamping plate, a connecting plate, a base plate, an injection needle penetrating hole, a telescopic rod and a spring, an injector is conveniently limited through the clamping plate and the injection needle penetrating hole during sample application, the stability of the injector during sample application is improved, and a sample is conveniently and uniformly distributed in gel.

Description

Technical Field

[0001] The utility model relates to the field of agarose gel, in particular to a gel hole alignment structure used for agarose gel electrophoresis spotting. Background Art

[0002] Agarose gel electrophoresis is a laboratory technique used to separate and identify DNA or RNA fragments by size. It is a fundamental and widely used experimental method in molecular biology and genetic engineering. This technique utilizes the migration properties of nucleic acid molecules in an electric field and the molecular sieving properties of agarose gel to separate nucleic acid molecules of different sizes and configurations.

[0003] In molecular biology experiments, especially when using agarose gel electrophoresis, ensuring that samples are accurately and correctly added to the gel wells is crucial. This involves the question of whether the gel wells need to be aligned. In fact, this is to ensure that the sample can move evenly and directly under the action of the electric field, avoiding sample mixing or loss, thereby obtaining clear and reproducible electrophoresis results.

[0004] When conducting agarose gel electrophoresis, samples need to be injected into the gel holes to facilitate subsequent experiments. When spotting, staff are required to hold the syringe and insert the needle of the syringe into the gel. The staff are required to keep their hands steady during injection, which requires high professional quality of the staff. The injection is prone to deviation, affecting the uniform distribution of the sample. Utility Model Content

[0005] In order to overcome the disadvantage of the prior art of injecting samples into gels, which is prone to injection deviation, one of the objectives of the present invention is to provide a gel hole alignment structure for agarose gel electrophoresis spotting.

[0006] One of the objectives of the utility model is achieved by the following technical solution: a gel hole alignment structure for agarose gel electrophoresis spotting, comprising a placement box: two vertical plates are fixedly connected to the upper side of the left end of the placement box, a rotating rod is rotatably connected between the two vertical plates, a rotating block is fixedly connected to the outer side of the rotating rod, a rotating plate is fixedly connected to the lower side of the rotating block, and a plurality of alignment units are provided on the upper side of the rotating plate;

[0007] The cam is secured to the upper edge of the chassis and is secured to a location where the cam is secured to the chassis's upper edge.

[0008] According to the gel hole alignment structure for agarose gel electrophoresis spotting, the connecting plates on the left and right sides are arranged in an obliquely symmetrical structure, which facilitates the control of the left and right clamping plates to move toward each other.

[0009] According to the gel hole alignment structure for agarose gel electrophoresis spotting, the mounting block is arranged in an L-shaped structure.

[0010] According to the gel hole alignment structure for agarose gel electrophoresis spotting, the clamping plate is arranged in an arc-shaped structure, which is convenient for limiting the outer side of the syringe.

[0011] According to the gel hole alignment structure for agarose gel electrophoresis spotting, the spring is sleeved on the outside of the telescopic rod.

[0012] According to the gel well alignment structure for agarose gel electrophoresis spotting, the transfer block is arranged in an L-shaped structure.

[0013] Beneficial effects:

[0014] By providing a rotating plate, a circular hole, a sliding hole, a slider, a clamping plate, a connecting plate, a chassis, an injection needle perforation, a telescopic rod and a spring, the syringe can be limited by the clamping plate and the injection needle perforation during sample spotting, thereby increasing the stability of the syringe during sample spotting and facilitating uniform distribution of the sample in the gel.

[0015] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0017] Figure 1 This is a three-dimensional structural diagram of the overall structure of a gel hole alignment structure for agarose gel electrophoresis spotting in the utility model;

[0018] Figure 2 This is a cross-sectional three-dimensional structural diagram of a transfer plate in a gel hole alignment structure for agarose gel electrophoresis spotting according to the present invention;

[0019] Figure 3 This is a three-dimensional structural diagram of an alignment unit of a gel hole alignment structure for agarose gel electrophoresis spotting in the utility model;

[0020] Figure 4 for Figure 3 Enlarged view of point A in the middle.

[0021] Legend:

[0022] 1. Placement box; 2. Vertical plate; 3. Rotating rod; 4. Rotating block; 5. Rotating plate; 6. Alignment unit; 601. Round hole; 602. Sliding hole; 603. Sliding block; 604. Mounting block; 605. Clamping plate; 606. Connecting block 1; 607. Connecting plate; 608. Connecting block 2; 609. Chassis; 610. Injection needle piercing hole; 611. Fixing block; 612. Telescopic rod; 613. Spring. DETAILED DESCRIPTION

[0023] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0024] Reference Figure 1-4 A gel hole alignment structure for agarose gel electrophoresis spotting includes a placement box 1: two vertical plates 2 are fixedly connected to the upper side of the left end of the placement box 1, a rotating rod 3 is rotatably connected between the two vertical plates 2, a rotating block 4 is fixedly connected to the outer side of the rotating rod 3, and the rotating block 4 is L-shaped. A rotating plate 5 is fixedly connected to the lower side of the rotating block 4, and a plurality of alignment units 6 are provided on the upper side of the rotating plate 5. When spotting is required, the rotating plate 5 is conveniently rotated through the vertical plate 2, the rotating rod 3 and the rotating block 4 so that the rotating plate 5 is located on the upper side of the placement box 1.

[0025] The alignment unit 6 includes a circular hole 601, which is opened on the upper side of the rotating plate 5. Sliding holes 602 are opened on the upper side of the rotating plate 5 and on the left and right sides of the circular hole 601. Slide blocks 603 are slidably connected to the inner sides of the sliding holes 602 on both sides. The upper sides of the left and right slide blocks 603 are fixedly connected to the mounting blocks 604. The outer sides of the mounting blocks 604 are fixedly connected to the splints 605. The lower sides of the left and right slide blocks 603 are fixedly connected to the connecting blocks 1 606. The outer side of the connecting block 606 is rotatably connected to the connecting plate 607, and the outer side of the lower end of the connecting plate 607 is rotatably connected to the connecting block 608. The lower side of the connecting block 608 is fixedly connected to the chassis 609. The lower side of the chassis 609 is provided with an injection needle through hole 610. The outer side of the chassis 609 is fixedly connected to the fixing block 611. The fixing block 611 and the rotating plate 5 are fixedly connected to the telescopic rod 612 and the spring 613. The connecting plates 607 on the left and right sides are tilted symmetrical structures. The mounting block 604 is an L-shaped structure, the splint 605 is an arc-shaped structure, and the spring 613 is sleeved on the outer side of the telescopic rod 612. When in use, the injection needle on the syringe is first passed through the injection needle hole 610 through the chassis 609, and the lower end of the syringe is against the upper side of the chassis 609. Then the syringe is pressed down, so that the syringe drives the chassis 609 to descend, and the chassis 609 drives the connecting plate 607 to rotate. Since the two connecting plates 607 are arranged in an obliquely symmetrical structure, it is convenient to control the sliders 603 on the left and right sides to approach each other, the slider 603 drives the mounting block 604 to move, and the mounting block 604 drives the splint 605 to move, so that the splint 605 on the left and right sides are close to each other, so that the splint 605 clamps and limits the outer side of the syringe, increases the stability of the syringe during injection, increases the stability of the sample, and performs positioning to avoid injection deviation. Under the action of the spring 613, the syringe is pulled out to reset the chassis 609.

[0026] Working principle: When in use, the staff first rotates the rotating plate 5 so that the rotating plate 5 is located on the upper side of the placement box 1, and then passes the injection needle of the syringe through the chassis 609, and then presses down the syringe to make the chassis 609 drop. Under the action of the connecting plate 607, it is convenient to control the left and right side clamps 605 to approach each other, so that the clamps 605 can limit the syringe, increase the stability of the syringe injecting the sample, avoid injection deviation, and then pull out the syringe. Under the action of the spring 613, it is convenient to reset the chassis 609 for the next use.

[0027] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A gel hole alignment structure for agarose gel electrophoresis spotting, characterized in that: The invention comprises a placement box (1): two vertical plates (2) are fixedly connected to the upper side of the left end of the placement box (1); a rotating rod (3) is rotatably connected between the two vertical plates (2); a rotating block (4) is fixedly connected to the outer side of the rotating rod (3); a rotating plate (5) is fixedly connected to the lower side of the rotating block (4); and a plurality of alignment units (6) are provided on the upper side of the rotating plate (5); The alignment unit (6) includes a circular hole (601), the circular hole (601) is opened on the upper side of the rotating plate (5), and sliding holes (602) are opened on the upper side of the rotating plate (5) and on the left and right sides of the circular hole (601). The inner sides of the sliding holes (602) on the left and right sides are slidably connected with sliders (603), the upper sides of the sliders (603) on the left and right sides are fixedly connected with mounting blocks (604), the outer sides of the mounting blocks (604) are fixedly connected with splints (605), and the lower sides of the sliders (603) on the left and right sides are fixedly connected with Connecting block one (606), the outer side of the connecting block one (606) is rotatably connected to a connecting plate (607), the outer side of the lower end of the connecting plate (607) is rotatably connected to a connecting block two (608), the lower side of the connecting block two (608) is fixedly connected to a chassis (609), the lower side of the chassis (609) is provided with an injection needle perforation (610), the outer side of the chassis (609) is fixedly connected to a fixed block (611), and a telescopic rod (612) and a spring (613) are fixedly connected between the fixed block (611) and the rotating plate (5).

2. The gel hole alignment structure for agarose gel electrophoresis spotting according to claim 1, characterized in that: The connecting plates (607) on the left and right sides are arranged in an obliquely symmetrical structure.

3. The gel hole alignment structure for agarose gel electrophoresis spotting according to claim 1, characterized in that: The mounting block (604) is arranged in an L-shaped structure.

4. The gel hole alignment structure for agarose gel electrophoresis spotting according to claim 1, characterized in that: The clamping plate (605) is arranged in an arc-shaped structure.

5. The gel hole alignment structure for agarose gel electrophoresis spotting according to claim 1, characterized in that: The spring (613) is sleeved on the outside of the telescopic rod (612).

6. The gel hole alignment structure for agarose gel electrophoresis spotting according to claim 1, characterized in that: The rotating block (4) is arranged in an L-shaped structure.

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