Device for efficiently obtaining exosomes secreted by mesenchymal stem cells

Abstract

The utility model discloses a device for efficiently obtaining exosomes secreted by mesenchymal stem cells, and relates to the biomedical field, the device comprises a box body, mounting blocks and a protective shell on two sides and a stretching mechanism for controlling a water spraying pipe, the tops of two sides of the outer wall of the box body are both provided with through holes, the two mounting blocks are both provided with holes identical to the through holes, and the protective shell is arranged in the through holes. A sealing door is installed on the face, away from the box body, of the protective shell through screws, an electric telescopic cylinder is connected to the end, away from the box body, of the water spraying pipe, and each stretching mechanism is composed of a sealing assembly and a pushing assembly. The sealing assembly comprises two U-shaped plates arranged on the mounting block in a sliding mode, fixing blocks are welded to the faces, close to the inner side wall of the protective shell, of the outer walls of the two U-shaped plates, the pushing assembly comprises two pushing plates and a side plate, horizontal clamping plates are arranged in the two pushing plates in a penetrating mode, and the ends of the two clamping plates are arranged on sliding rods in a sleeving mode. According to the exosome preparation device, the risk that microorganisms accumulated in an atomizing nozzle pollute a culture solution when the atomizing nozzle is arranged at the top can be effectively reduced or avoided, so that the quality of the preparation process is ensured, and the exosome obtaining efficiency is improved.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of biomedicine, and specifically is a device for efficiently obtaining exosomes secreted by mesenchymal stem cells. Background Art

[0002] Mesenchymal stem cells (MSCs) are a type of cell capable of differentiating into various cell types, including osteoblasts, chondrocytes, adipocytes, and white blood cells. Their multipotent differentiation capacity, ease of rapid isolation, and ease of delivery have led to their application in treating mesenchymal tissue damage and in hematopoietic stem cell transplantation. Furthermore, the exosomes secreted by MSCs are structurally stable and resistant to degradation.

[0003] A method and device for preparing mesenchymal stem cell exosomes described in the prior art include a box, a liquid inlet and a liquid outlet. The box is provided with a liquid inlet on the top and a liquid outlet on the bottom. A liquid inlet valve is installed on the surface of the liquid inlet, and a liquid outlet valve is installed on the surface of the liquid outlet. A stirring device is fixedly connected to the top of the box, a humidifying device is rotatably installed on the lower surface of the top of the box, the humidifying device is fixedly connected to the stirring device, a water storage tank is fixedly connected to the surface of the water storage tank, a first connecting pipe is fixedly connected to the surface of the water storage tank, the first connecting pipe is connected to the interior of the water storage tank, and the end of the first connecting pipe away from the water storage tank is fixedly connected to the humidifying device. A temperature sensor, a humidity sensor and a carbon dioxide sensor are installed on the lower surface of the top of the box.

[0004] Although the above technology can ensure the humidity, temperature and carbon dioxide concentration inside the box to avoid affecting the culture effect, it has a simple structure and strong practicality. However, an atomizing nozzle is installed on the top, and the microorganisms accumulated in the atomizing nozzle may cause the risk of contamination of the culture fluid, thereby affecting the quality of the preparation process and reducing the efficiency of subsequent exosome acquisition. Utility Model Content

[0005] Based on this, the purpose of the present invention is to provide a device for efficiently obtaining exosomes secreted by mesenchymal stem cells, so as to solve the technical problems raised in the above background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] A device for efficiently harvesting exosomes secreted by mesenchymal stem cells comprises a housing, mounting blocks on both sides, a protective housing, and an extension mechanism for controlling a water spray pipe. The top of each side of the housing's outer wall is provided with a perforation, and both mounting blocks are provided with holes identical to the perforations. A sealing door is screwed onto the side of the protective housing away from the housing. The end of the water spray pipe away from the housing is connected to an electric telescopic cylinder. Each extension mechanism comprises a sealing component and a driving component.

[0008] The closure assembly includes two U-shaped plates slidably arranged on the mounting blocks, a fixed block is welded to one side of the outer wall of the two U-shaped plates close to the inner wall of the protective shell, an arched plate is welded to the outer wall of the two U-shaped plates at the fixed block, the two fixed blocks are connected to a clamping block through a movable plate, the water spray pipe is arranged between the two clamping blocks, the two ends of the two movable plates are rotatably connected to the fixed block and the clamping block respectively, and a stopper is fixed to one side of the two clamping blocks away from the U-shaped plate;

[0009] The pushing assembly includes two push plates and side plates, and a horizontal clamping plate is passed through the two push plates. The ends of the two clamping plates are both mounted on the sliding rod, and top blocks are fixed at both ends of the two sliding rods. Guide blocks are fixed at the bottom ends of the two top blocks, and the bottom ends of the two guide blocks are both mounted on the guide rods. The two ends of each guide rod are respectively fixedly connected to the outer wall of the side plate and the inner wall of the protective shell, and the two arched plates are matched with the top blocks.

[0010] Specifically, the two U-shaped plates are bonded with rubber pads on one side away from the inner wall of the protective shell, and limiting blocks are fixed on the side of the U-end inner wall of the two U-shaped plates close to the rubber pad. A limiting groove is provided at the top and bottom of each mounting block, and each limiting block is slidably connected to the limiting groove. A ball is embedded in one side of each limiting block located at the bottom of the limiting groove.

[0011] Specifically, the technical solution of the present invention is provided with sliding grooves on both sides of the outer wall of the water spray pipe, and sliders are slidably installed in the two sliding grooves, and the two sliders are fixedly connected to the outer wall of the block.

[0012] Specifically, the end of the water spray pipe away from the U-shaped plate is provided with an electric telescopic cylinder, the telescopic end of the electric telescopic cylinder is connected to the end of the water spray pipe by a bolt, the bottom end of the electric telescopic cylinder is connected to the inner wall of the sealing door by a bolt, and a plurality of atomizing nozzles are evenly distributed on the outer wall of the water spray pipe.

[0013] Specifically, the two push plates and the stop block are fixedly connected by a connecting block, and clamping blocks are fixed on both sides of the end of the water spray pipe away from the U-shaped plate. The ends of the two clamping plates away from the sliding rod are plugged into the clamping blocks, and each guide rod is provided with a contraction spring, and the two ends of each contraction spring are fixedly connected to the side plate and the outer wall of the guide block.

[0014] The technical solution is specific. A slot block is provided directly below the two connecting blocks. The bottom ends of the two push plates are fixedly connected to the vertical plates. The bottom ends of the two vertical plates are slidingly connected to the slots of the slot blocks. Tension springs are provided in the slots of the two slot blocks. Both ends of the two tension springs are fixedly connected to the inner wall of the slot and the outer wall of the vertical plates. Magnets are fixedly installed on the end walls of the slots of the two slot blocks. The diameters of the two magnets are smaller than the diameter of the tension springs, and the two magnets are magnetically connected to the outer wall of the bottom end of the vertical plates.

[0015] Specifically, the inner bottom wall of the protective shell is fixed with a water tank, the bottom ends of the two trough blocks are fixedly connected to the top of the water tank, the bottom ends of the two side panels are fixedly connected to the top of the water tank, and the end face of the water tank is provided with a water filling pipe.

[0016] Specifically, the water supply pipe passes through the sealed door and extends to the outside. A water pump is installed in the water tank through bolts. The water outlet of the water pump is connected to the water spray pipe through a telescopic hose.

[0017] Specifically, the top opening of the box body is installed with a top cover by bolts, the top cover is provided with a liquid inlet pipe, the bottom center of the box body is connected with a liquid outlet pipe, and solenoid valves are installed in the liquid inlet pipe and the liquid outlet pipe.

[0018] Specifically, a stirring motor is installed at the center of the upper surface of the top cover by bolts, the output end of the stirring motor passes through the top cover and is connected to a stirring rod by a flange, and a humidity sensor is installed on one side of the lower surface of the top cover.

[0019] In summary, the present invention has the following beneficial effects: the present invention can effectively reduce or avoid the risk of contamination of the culture fluid by microorganisms accumulated in the atomizing nozzle when the atomizing nozzle is set on the top, thereby ensuring the quality of the preparation process and improving the efficiency of obtaining exosomes;

[0020] At the same time, it can also ensure good sealing of the box, reduce the possibility of microorganisms from the external air entering, and facilitate maintenance of the structure and water pipes to ensure stable performance;

[0021] When the water level is lower than the preset range, the electric telescopic cylinder is started, and its telescopic end pushes the water spray pipe to move. The water spray pipe drives the connected card plate to move through the card block, so that the card plate pushes the stop block and the clamping block through the push plate and the connecting block, and drives the vertical plate to move along the slot block, and the other end of the card plate will slide along the slide rod. When the clamping block follows the water spray pipe to move, as it approaches the box, it will push the fixed block to move to both sides through the movable plate, and the fixed block will drive the U-shaped plate to move along the mounting block, so that the hole is exposed, until the arched plate on the U-shaped plate contacts and pushes the top block. The top block will drive the other top block to move through the slide rod, and the slide rod drives the card plate to move. At this time, the end of the card plate will be separated from the card block. At this time, the movement of the water spray pipe will no longer drive the clamping block to move, and the vertical plate will be attracted by the magnet to limit its position.

[0022] Then the telescopic end of the electric telescopic cylinder continues to expand, pushing the water spray pipe through the holes and perforations into the interior of the box. As the water spray pipe moves, the telescopic hose will be stretched. At this time, the water pump will work to send the deionized water in the water tank into the water spray pipe through the telescopic hose, and spray it out from several atomizing nozzles until the humidity inside the box returns to the preset value. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is an overall schematic diagram of the utility model.

[0024] Figure 2 This is a cross-sectional view of the box body and protective shell of the utility model.

[0025] Figure 3 This is a schematic diagram of the installation block of the present utility model.

[0026] Figure 4 This is a structural diagram of the extension mechanism of the present utility model.

[0027] Figure 5 For the utility model Figure 4 Top view.

[0028] Figure 6 This is a schematic diagram of a U-shaped plate of the present invention.

[0029] Figure 7 For the utility model Figure 4 Main view.

[0030] Description of the drawings: 1. Box body; 101. Stirring motor; 102. Stirring rod; 103. Liquid inlet pipe; 104. Liquid outlet pipe; 105. Perforation; 106. Top cover; 2. Mounting block; 201. Hole; 202. Restriction groove; 3. Protective shell; 301. Sealing door; 302. Water storage tank; 3021. Water pump; 3022. Water supply pipe; 303. Water spray pipe; 3031. Atomizing nozzle; 3032. Slide; 304. Electric telescopic cylinder; 305. Telescopic hose; 306. Clamping block; 4. Extension mechanism; 5. Closing assembly; 501. U-shaped plate; 5011, limiting block; 5012, ball bearing; 5013, rubber pad; 502, arch plate; 503, fixed block; 504, clamping block; 505, movable plate; 506, stop block; 5061, slider; 6, pushing assembly; 601, connecting block; 602, push plate; 6021, vertical plate; 603, clamping plate; 604, slot block; 6041, magnet; 6042, tension spring; 605, side plate; 6051, guide rod; 6052, contraction spring; 6053, guide block; 606, top block; 6061, slide rod. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0032] The following describes an embodiment of the present invention based on its overall structure. Example

[0033] All electrical components in this application are controlled by an external controller, and the humidity range in the box 1 is set by the controller.

[0034] See also Figure 1-3 As shown, a device for efficiently obtaining exosomes secreted by mesenchymal stem cells includes a housing 1, mounting blocks 2 and a protective shell 3 on both sides, and an extension mechanism 4 for controlling a water spray pipe 303. A top cover 106 is bolted to the top opening of the housing 1. A liquid inlet pipe 103 is provided on the top cover 106. A liquid outlet pipe 104 is connected to the center of the bottom end of the housing 1. Solenoid valves are installed in the liquid inlet pipe 103 and the liquid outlet pipe 104. A stirring motor 101 is bolted to the center of the upper surface of the top cover 106. The output end of the stirring motor 101 passes through the top cover 106 and is connected to a stirring rod 102 via a flange. A humidity sensor is installed on one side of the lower surface of the top cover 106.

[0035] The top of both sides of the outer wall of the box body 1 is provided with a through-hole 105, and the two mounting blocks 2 are provided with a hole 201 identical to the through-hole 105. A sealing door 301 is installed with screws on the side of the protective shell 3 away from the box body 1. An electric telescopic cylinder 304 is provided at the end of the water spray pipe 303 away from the U-shaped plate 501. The telescopic end of the electric telescopic cylinder 304 is connected to the end of the water spray pipe 303 by bolts, and the bottom end of the electric telescopic cylinder 304 is connected to the inner wall of the sealing door 301 by bolts. A plurality of atomizing nozzles 3031 are evenly distributed on the outer wall of the water spray pipe 303. The end of the water spray pipe 303 away from the box body 1 is connected to the electric telescopic cylinder 304. Each extension mechanism 4 is composed of a closing component 5 and a pushing component 6.

[0036] When extracting exosomes from mesenchymal stem cells, the mesenchymal stem cells need to be prepared. A gelatin aqueous solution is taken and a crude gelatin microsphere is prepared using the double emulsion method. The crude gelatin microsphere is dehydrated with anhydrous ethanol and then cross-linked in an anhydrous ethanol solution containing genipin to prepare gelatin microcarriers.

[0037] A culture medium containing gelatin microcarriers is placed inside the box 1, and mesenchymal stem cells are inoculated into the culture medium. The stirring motor 101 drives the stirring rod 102 to rotate slowly for stirring culture. When the cells attached to the gelatin microcarriers grow to a confluence of 80% to 90%, recombinant interferon γ and cytochalasin D are added, and the culture is continued for 12 to 48 hours. The conditioned medium is collected and exosomes are extracted. The concentration of recombinant interferon γ is 10-50 μg / L, and the concentration of cytochalasin D is 0.5-2.0 μg / ml;

[0038] The humidity sensor detects in real time whether the humidity in the box 1 is less than a preset range. When it is detected that the humidity is less than the preset range, the controller starts the electric telescopic cylinder 304 and the water pump 3021 in sequence through the built-in program. The telescopic end of the electric telescopic cylinder 304 pushes the water spray pipe 303 to move. When the water spray pipe 303 moves, it drives the closing component 5 to expand through the pushing component 6, so that the hole 201 and the through-hole 105 are exposed. When the closing component 5 is expanded to the maximum, it also drives the pushing component 6 to move horizontally, so that the pushing component 6 is separated from the water spray pipe 303. At this time, the water spray pipe 303 passes through the hole 201 and the through-hole 105 under the action of the electric telescopic cylinder 304 and enters the interior of the box 1. As the water spray pipe 303 moves, the telescopic hose 305 is stretched. At this time, the water pump 3021 works to send the deionized water in the water tank 302 into the water spray pipe 303 through the telescopic hose 305, and sprays it from the multiple atomizing nozzles 3031 until the humidity inside the box 1 returns to the preset value.

[0039] When regular maintenance is required after long-term use, the staff can use tools to separate the electric telescopic cylinder 304 from the sealing door 301, and then remove the sealing door 301 to expose the extension mechanism 4 inside the protective shell 3, making it easier to maintain the water spray pipe 303, the closing component 5 and the pushing component 6;

[0040] Therefore, the present application can effectively reduce or avoid the risk of microorganisms accumulated in the atomizing nozzle 3031 for a long time causing contamination of the culture fluid, thereby ensuring the quality of the preparation process and improving the efficiency of obtaining exosomes. At the same time, it can also ensure that the box 1 is well sealed, reduce the possibility of microorganisms from the external air entering, and facilitate maintenance of the structure and the water spray pipe 303 to ensure stable performance.

[0041] See also Figure 3-7 As shown, the closing assembly 5 includes two U-shaped plates 501 slidably arranged on the mounting block 2, and the outer walls of the two U-shaped plates 501 are welded with fixed blocks 503 on one side close to the inner wall of the protective shell 3, and the outer walls of the two U-shaped plates 501 are welded with arched plates 502 at the fixed blocks 503. The two fixed blocks 503 are connected to the clamping blocks 504 through the movable plates 505. The water spray pipe 303 is arranged between the two clamping blocks 504. The two ends of the two movable plates 505 are rotatably connected to the fixed blocks 503 and the clamping blocks 504 respectively. The two clamping blocks 504 are fixed with stoppers 506 on one side away from the U-shaped plates 501. A rubber pad 5013 is bonded to the side away from the inner wall of the protective shell 3. A limiting block 5011 is fixed to the inner wall of the U end of each U-shaped plate 501 on the side near the rubber pad 5013. A limiting groove 202 is defined at the top and bottom of each mounting block 2. Each limiting block 5011 is slidably connected to the limiting groove 202. A ball bearing 5012 is embedded in the side of each limiting block 5011 located at the bottom of the limiting groove 202. A sliding groove 3032 is defined on both sides of the outer wall of the water spray pipe 303. Slide blocks 5061 are slidably installed in each sliding groove 3032. Both slide blocks 5061 are fixedly connected to the outer wall of the stop block 506.

[0042] The pushing assembly 6 includes two push plates 602 and side plates 605. A horizontal clamping plate 603 is inserted into the two push plates 602. The ends of the two clamping plates 603 are both mounted on the slide rod 6061. Both ends of the two slide rods 6061 are fixed with a top block 606. The bottom ends of the two top blocks 606 are fixed with a guide block 6053. The bottom ends of the two guide blocks 6053 are both mounted on the guide rod 6051. The two ends of each guide rod 6051 are fixedly connected to the outer wall of the side plate 605 and the inner wall of the protective shell 3 respectively. The two arched plates 502 are matched with the top block 606, and the two push plates 602 are fixedly connected to the stop block 506 through the connecting block 601. The ends of the water spray pipe 303 away from the U-shaped plate 501 are fixed with the clamping blocks 306 on both sides. The ends of the two clamping plates 603 away from the sliding rod 6061 are plug-connected with the clamping blocks 306. Each guide rod 6051 is sleeved with a contraction spring 6052, and the two ends of each contraction spring 6052 are fixedly connected to the side plate 605 and the outer wall of the guide block 6053 respectively.

[0043] A slot block 604 is provided directly below the two connecting blocks 601. The bottom ends of the two push plates 602 are fixedly connected to a vertical plate 6021. The bottom ends of the two vertical plates 6021 are slidably connected to the slots of the slot blocks 604. Tension springs 6042 are provided in the slots of the two slot blocks 604. Both ends of the two tension springs 6042 are fixedly connected to the inner wall of the slot and the outer wall of the vertical plate 6021. Magnets 6041 are fixedly installed on the end walls of the slots of the two slot blocks 604. The diameters of the two magnets 6041 are smaller than the diameters of the tension springs 6042, and the two magnets 6041 are magnetically connected to the outer wall of the bottom end of the vertical plate 6021.

[0044] A water tank 302 is fixed to the inner bottom wall of the protective shell 3, the bottom ends of the two slot blocks 604 are fixedly connected to the top of the water tank 302, and the bottom ends of the two side panels 605 are fixedly connected to the top of the water tank 302. A water supply pipe 3022 is provided on the end face of the water tank 302, and the water supply pipe 3022 extends to the outside through the sealed door 301. A water pump 3021 is installed in the water tank 302 by bolts, and the water outlet of the water pump 3021 is connected to the water spray pipe 303 through a telescopic hose 305.

[0045] When the water spray pipe 303 moves, the water spray pipe 303 will drive the plugged clamping plate 603 to move through the clamping block 306, and the clamping plate 603 will move along the sliding rod 6061 and drive the push plate 602 to move, and the push plate 602 will drive the connecting block 601 and the vertical plate 6021 to move, and the connecting block 601 will push the stop block 506 and the clamping block 504 to move, and the vertical plate 6021 will move along the groove block 604 and squeeze the tension spring 6042. When the clamping block 504 moves along the water spray pipe 303 and approaches the box body 1, it will push the fixed block 503 to move to both sides through the movable plate 505, and the fixed block 503 will drive the U-shaped plate 501 to move to both sides along the mounting block 2, and the limiting block 5011 will move along the limiting groove 202 through the ball 5012, so that the hole 201 is exposed;

[0046] At this time, the U-shaped plate 501 will also drive the arch plate 502 to move synchronously until the outer wall of the arch plate 502 contacts a top block 606. As the U-shaped plate 501 continues to be pushed, the arch plate 502 will push the top block 606 to move, and the top block 606 will drive the other top block 606 to move through the sliding rod 6061. The sliding rod 6061 drives the clamping plate 603 to move, so that the end of the clamping plate 603 is separated from the clamping block 306, and the movement of the top block 606 will also drive the guide block 6053 at the bottom to move along the guide rod 6051 and stretch the contraction spring 6052. At this time, the two vertical plates 6021 will contact the magnet 6041 and be adsorbed to complete the restricted positioning of the pushing component 6. At this time, the movement of the water spray pipe 303 will not continue to drive the push plate 602 and the clamping block 504 to move.

[0047] The working principle of this utility model is:

[0048] The humidity sensor detects in real time whether the humidity in the box 1 is less than the preset range. When it is detected that the humidity is less than the preset range, the controller starts the electric telescopic cylinder 304 and the water pump 3021 in sequence through the built-in program. The telescopic end of the electric telescopic cylinder 304 pushes the water spray pipe 303 to move. The water spray pipe 303 drives the plugged card plate 603 to move through the card block 306. The card plate 603 moves along the slide rod 6061 and drives the push plate 602 to move. The push plate 602 drives the connecting block 601 and the vertical plate 6021 to move. When the U-shaped plate 501 is moved, the connecting block 601 pushes the stopper 506 and the clamping block 504 to move, and the vertical plate 6021 moves along the groove block 604 and squeezes the tension spring 6042. When the clamping block 504 moves along the water spray pipe 303 and approaches the box body 1, it pushes the fixed block 503 to move to both sides through the movable plate 505. The fixed block 503 drives the U-shaped plate 501 to move to both sides along the mounting block 2. The limiting block 5011 moves along the limiting groove 202 via the ball bearing 5012, so that the hole 201 is exposed.

[0049] At this time, the U-shaped plate 501 will also drive the arch plate 502 to move synchronously until the outer wall of the arch plate 502 contacts a top block 606. As the U-shaped plate 501 continues to be pushed, the arch plate 502 will push the top block 606 to move, and the top block 606 will drive another top block 606 to move through the sliding rod 6061. The sliding rod 6061 drives the clamping plate 603 to move, so that the end of the clamping plate 603 is separated from the clamping block 306. The movement of the top block 606 will also drive the guide block 6053 at the bottom to move along the guide rod 6051 and stretch the contraction spring 6052. At this time, the two vertical plates 6021 will The push assembly 6 comes into contact with the magnet 6041 and is adsorbed, completing the restricted positioning of the push assembly 6. At this time, the movement of the water spray pipe 303 will no longer drive the push plate 602 and the clamping block 504 to move. At this time, the water spray pipe 303 will pass through the hole 201 and the through-hole 105 under the action of the electric telescopic cylinder 304 and enter the interior of the box 1. As the water spray pipe 303 moves, the telescopic hose 305 will be stretched. At this time, the water pump 3021 will work to send the deionized water in the water tank 302 into the water spray pipe 303 through the telescopic hose 305, and spray it out from the multiple atomizing nozzles 3031 until the humidity inside the box 1 returns to the preset value.

[0050] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not limitations on the present invention. The specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and purpose of the present invention, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A device for efficiently obtaining exosomes secreted by mesenchymal stem cells, comprising a housing (1), mounting blocks (2) on both sides, a protective shell (3), and an extension mechanism (4) for controlling a water spray pipe (303), characterized in that The top of both sides of the outer wall of the box body (1) is provided with a through hole (105), and the two mounting blocks (2) are provided with a hole (201) identical to the through hole (105). A sealing door (301) is installed on the side of the protective shell (3) away from the box body (1) by screws. The end of the water spray pipe (303) away from the box body (1) is connected to an electric telescopic cylinder (304), and each of the extension mechanisms (4) is composed of a closing component (5) and a pushing component (6); The closing assembly (5) comprises two U-shaped plates (501) slidably arranged on the mounting block (2), a fixed block (503) is welded to one side of the outer wall of the two U-shaped plates (501) close to the inner wall of the protective shell (3), an arched plate (502) is welded to the outer wall of the two U-shaped plates (501) at the fixed block (503), the two fixed blocks (503) are connected to a clamping block (504) via a movable plate (505), the water spray pipe (303) is arranged between the two clamping blocks (504), the two ends of the two movable plates (505) are rotatably connected to the fixed block (503) and the clamping block (504), and a stopper (506) is fixed to one side of the two clamping blocks (504) away from the U-shaped plate (501); The pushing assembly (6) includes two push plates (602) and side plates (605), a horizontal snap-in plate (603) is inserted into the two push plates (602), the ends of the two snap-in plates (603) are both mounted on the slide rod (6061), both ends of the two slide rods (6061) are fixed with a top block (606), the bottom ends of the two top blocks (606) are fixed with a guide block (6053), the bottom ends of the two guide blocks (6053) are both mounted on the guide rod (6051), the two ends of each guide rod (6051) are respectively fixedly connected to the outer wall of the side plate (605) and the inner wall of the protective shell (3), and the two arched plates (502) are matched with the top block (606).

2. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 1, characterized in that: A rubber pad (5013) is bonded to one side of the two U-shaped plates (501) away from the inner side wall of the protective shell (3); a limiting block (5011) is fixed to one side of the inner wall of the U end of the two U-shaped plates (501) close to the rubber pad (5013); a limiting groove (202) is provided at the top and bottom of each mounting block (2); each limiting block (5011) is slidably connected to the limiting groove (202); and a ball (5012) is embedded on one side of each limiting block (5011) located at the bottom of the limiting groove (202).

3. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 1, characterized in that: Slide grooves (3032) are provided on both sides of the outer wall of the water spray pipe (303), and sliders (5061) are slidably installed in the two slide grooves (3032). The two sliders (5061) are fixedly connected to the outer wall of the stopper (506).

4. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 1, characterized in that: An electric telescopic cylinder (304) is provided at the end of the water spray pipe (303) away from the U-shaped plate (501); the telescopic end of the electric telescopic cylinder (304) is connected to the end of the water spray pipe (303) via a bolt; the bottom end of the electric telescopic cylinder (304) is connected to the inner wall of the sealing door (301) via a bolt; and a plurality of atomizing nozzles (3031) are evenly distributed on the outer wall of the water spray pipe (303).

5. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 1, characterized in that: The two push plates (602) are fixedly connected to the stop block (506) via a connecting block (601); the ends of the water spray pipe (303) away from the U-shaped plate (501) are fixed with a clamping block (306); the ends of the two clamping plates (603) away from the slide rod (6061) are plug-connected to the clamping block (306); each guide rod (6051) is provided with a contraction spring (6052); and the two ends of each contraction spring (6052) are fixedly connected to the side plate (605) and the outer wall of the guide block (6053).

6. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 5, characterized in that: A slot block (604) is provided directly below the two connecting blocks (601), the bottom ends of the two push plates (602) are fixedly connected to a vertical plate (6021), the bottom ends of the two vertical plates (6021) are slidably connected to the slots of the slot block (604), a tension spring (6042) is provided in the slots of the two slot blocks (604), both ends of the two tension springs (6042) are fixedly connected to the inner wall of the slot and the outer wall of the vertical plate (6021), a magnet (6041) is fixedly installed on the middle end wall of the slot of the two slot blocks (604), the diameter of the two magnets (6041) is smaller than the diameter of the tension spring (6042), and the two magnets (6041) are magnetically connected to the outer wall of the bottom end of the vertical plate (6021).

7. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 6, characterized in that: A water storage tank (302) is fixed to the inner bottom wall of the protective shell (3), the bottom ends of the two groove blocks (604) are fixedly connected to the top of the water storage tank (302), the bottom ends of the two side plates (605) are fixedly connected to the top of the water storage tank (302), and a water supply pipe (3022) is provided on the end surface of the water storage tank (302).

8. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 7, characterized in that: The water supply pipe (3022) passes through the sealed door (301) and extends to the outside. A water pump (3021) is installed in the water storage tank (302) via bolts. The water outlet of the water pump (3021) is connected to the water spray pipe (303) via a telescopic hose (305).

9. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 1, characterized in that: A top cover (106) is mounted on the top opening of the box body (1) via bolts. A liquid inlet pipe (103) is provided on the top cover (106). A liquid outlet pipe (104) is connected to the center of the bottom end of the box body (1). Solenoid valves are installed in both the liquid inlet pipe (103) and the liquid outlet pipe (104).

10. The device for efficiently obtaining exosomes secreted by mesenchymal stem cells according to claim 9, characterized in that: A stirring motor (101) is mounted at the center of the upper surface of the top cover (106) via bolts, an output end of the stirring motor (101) passes through the top cover (106) and is connected to a stirring rod (102) via a flange, and a humidity sensor is mounted on one side of the lower surface of the top cover (106).

Images