Cryopreservation bag

Abstract

The utility model discloses a cryopreservation bag which comprises a bag body, a guide pipe and a gas collection cabin. The bag body comprises a bag body for storing a biological sample; the catheter is communicated with the bag body, and the catheter is connected with one corner of the bag body; the gas collecting cabin is arranged on the guide pipe, and the gas collecting cabin is communicated with the bag body and the guide pipe; when the bubbles are clamped at the corners, the air exhaust mechanism exhausts air from the guide pipe, and the bubbles at the corners can be quickly exhausted and collected into the air collection cabin, so that the bagging efficiency of the biological samples can be accelerated, the extraction of excessive biological samples can be avoided, and the waste of the biological samples is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological sample storage containers, and particularly relates to a freezing bag. Background Art

[0002] Cryopreservation bags, also often called frozen storage bags or liquid nitrogen storage bags, are mainly used for long-term low-temperature storage of liquid biological samples such as cells, tissues, blood, sperm, and eggs;

[0003] After placing the biological sample into the freezing bag, the air inside the bag needs to be completely removed. If there is air in the bag, the air inside the bag will expand and form ice crystals due to the drop in temperature during the freezing process, which may cause the bag to rupture or poor sealing, and in serious cases, the biological sample will become ineffective. Therefore, completely removing the air from the bag is particularly important to maintain the activity of the biological sample.

[0004] In the prior art, due to the limitations of the cryopreservation bag structure, if the flow rate of the biological sample is not properly controlled during the process of the biological sample entering the bag along the pipeline, air is easily filled into the cryopreservation bag, and the air forms bubbles in the bag, and the bubbles are easily stuck in the corners of the bag. In order to expel all the bubbles in the bag, after the biological sample is bagged, a vacuum mechanism (such as a syringe) is generally used to squeeze the corners of the bag to expel all the bubbles in the bag. In this process, under the action of the vacuum mechanism, the biological sample in the part connected to the bubble is also extracted from the bag. The extracted biological sample is at risk of being contaminated, so it is necessary to discard the extracted biological sample, which reduces the utilization rate of the biological sample. Utility Model Content

[0005] The utility model aims to solve the above technical problems existing in the prior art, and provides a freezing bag that is easy to exhaust.

[0006] The technical solution adopted by the utility model to solve its technical problems is:

[0007] A freezing bag, comprising: a bag body, a catheter and a gas collecting chamber;

[0008] The bag is used to store biological samples;

[0009] The conduit is in communication with the bag body, and the conduit is connected to a corner of the bag body;

[0010] The gas collecting chamber is arranged on the conduit and is communicated with the bag body and the conduit.

[0011] In the cryopreservation bag as described above, the cross-sectional area of ​​the gas collecting chamber is larger than the cross-sectional area of ​​the conduit.

[0012] In the freezing bag as described above, the gas collecting chamber is a dripping funnel.

[0013] As described above, the freezing bag has a cone-like portion provided on one side of the bag body close to the conduit, and the conduit is connected to a vertex of the cone-like portion.

[0014] The freezing bag as described above further comprises a valve, wherein the conduit portion is placed in the valve, and the valve is arranged on a side of the gas collecting chamber away from the bag body.

[0015] In the freezing bag as described above, the valve is a switch valve or a flow control valve.

[0016] As described above, the freezing bag has a lowest surface at one end of the bag body away from the catheter, and a switch is connected to the bag body on the lowest surface, and the switch is used to allow the biological sample in the bag body to flow out.

[0017] As described above, the freezing bag has an inverted trapezoidal portion provided on one side of the bag body close to the switch, the lowest surface being the short side of the inverted trapezoidal portion, and the switches may be multiple and distributed along the short side of the inverted trapezoidal portion.

[0018] The cryopreservation bag as described above, wherein the conduit comprises a first conduit and a second conduit;

[0019] The first conduit is provided between the bag body and the gas collecting chamber;

[0020] The second conduit is provided at one end of the gas collecting chamber away from the bag body;

[0021] A flow sensor is provided on the first conduit.

[0022] As for the cryopreservation bag described above, both ends of the first conduit are respectively bonded to the bag body and the gas collecting chamber by a polymer adhesive, and the second conduit is connected to the gas collecting chamber by the polymer adhesive.

[0023] The beneficial effects of the utility model are:

[0024] An air collecting chamber is set on the catheter connected to the bag body, and the position where the catheter is connected to the bag body is set at the corner of the bag body. When bubbles are stuck in the corner, the suction mechanism draws air from the catheter or pats the bag body, which can quickly concentrate the bubbles at the corner into the air collecting chamber, thereby discharging the air in the bag body, speeding up the bagging efficiency of biological samples, avoiding the extraction of too many biological samples, and reducing the waste of biological samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0026] Figure 1 This is one of the structural diagrams of the present utility model;

[0027] Figure 2 This is the second structural diagram of the present utility model;

[0028] The reference numerals are as follows:

[0029] 1. Bag body; 11. Cone-shaped portion; 12. Lowest surface; 13. Inverted trapezoidal portion; 2. Conduit; 21. First conduit; 22. Second conduit; 3. Gas collecting chamber; 4. Valve; 5. Switch; 51. Plug body; 52. Fin. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the concept, specific structure and technical effects of the present invention in combination with the embodiments and drawings, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by technical personnel in this field without creative work are within the scope of protection of the present invention. In addition, all the connection / connection relationships involved in the patent do not refer to the direct connection of components, but refer to the fact that a better connection structure can be formed by adding or reducing connection accessories according to the specific implementation situation. The various technical features in the creation of the present invention can be combined interactively without conflicting with each other.

[0031] Cryopreservation bags are generally used to store liquid biological samples. The attraction between liquid molecules is greater than that between liquid molecules and air molecules, and greater than the attraction between liquid molecules and solid molecules. Therefore, when the liquid flows at the corners of the cryopreservation bag, the liquid in the cryopreservation bag has a tendency to shrink the liquid surface to the minimum surface area, which easily accumulates bubbles at the corners of the cryopreservation bag and forms flow dead corners. In order to expel bubbles from the corners of the cryopreservation bag, external force needs to be applied to the corners of the cryopreservation bag. When the horizontal position of the corners is higher than the connection position between the bag body and the catheter of the cryopreservation bag, it becomes more difficult to expel bubbles from the bag body.

[0032] The existing technology uses a method of squeezing bubbles in the bag while extracting the biological sample using a suction mechanism such as a syringe. When the bubbles enter the catheter, they form sections of air columns and liquid columns formed by the biological sample. The syringe is pulled to extract the air columns and liquid columns into the syringe together. This process extracts a large amount of biological sample and the operation takes a long time.

[0033] In order to solve the above problems, this embodiment provides a freezing bag, referring to Figures 1 to 2 , comprising: a bag body 1, a catheter 2 and a gas collecting chamber 3;

[0034] The conduit 2 is connected to the bag body 1, and the conduit 2 is connected to a corner of the bag body 1;

[0035] The gas collecting chamber 3 is disposed on the conduit 2 and is in communication with the bag body 1 and the conduit 2 .

[0036] When using the cryopreservation bag of this embodiment, the cryopreservation bag is placed in a vertical position. At this time, the gas collecting chamber 3 is located above the bag body 1. The biological sample (such as cell fluid) is poured into the bag body 1 along the conduit 2. The biological sample flows into the bag body 1 after passing through the gas collecting chamber 3. As the biological sample is continuously poured into the bag body 1, the bag body 1 is gradually filled, and the bubbles in the bag body 1 are concentrated in the corners. When bubbles are attached to the corners of the bag body 1, a variety of methods can be adopted to discharge the bubbles in the bag body 1. The following are two examples of exhaust methods:

[0037] First, squeeze the air bubbles in the bag 1 and gently tap the catheter 2 to vibrate the bag, which encourages the bubbles to move inside the bag and reduces their contact with the bag 1 and the inner wall of the catheter 2. Since the density of air is lower than that of liquid, the bubbles in the bag 1 can rise into the air collecting chamber 3, thereby expelling the air in the bag 1. From the outside, the liquid level of the biological sample is now located at the air collecting chamber 3.

[0038] Secondly, an air extraction mechanism (such as a syringe) is used. The air exhaust mechanism uses the conduit 2 as an interface to extract the bubbles in the bag 1, so that the bubbles are concentrated in the gas collecting chamber 3 to exhaust the air in the bag 1. From the appearance, the liquid level of the biological sample is also located at the gas collecting chamber 3 at this time.

[0039] Therefore, compared with the prior art, this freezing bag does not need to extract a large amount of biological samples in order to extract bubbles during operation, which can improve the utilization rate of biological samples; and from the above-mentioned exhaust method, it can be seen that the exhaust method of the freezing bag in this embodiment is simple and easy to operate, which can improve the work efficiency of the operator.

[0040] In one embodiment, the cross-sectional area of ​​the gas collecting chamber 3 is larger than the cross-sectional area of ​​the conduit 2. When the horizontal surface of the biological sample is located in the gas collecting chamber 3 and the conduit 2, respectively, the horizontal surface of the biological sample located in the gas collecting chamber 3 has a larger area, which will gather more air on the horizontal surface of the biological sample, helping to gather air in the gas collecting chamber 3 and reducing the probability of external air entering the bag body 1 during the process of bagging the biological sample.

[0041] Specifically, the gas collecting chamber 3 is a dripping funnel, which is a component commonly used in disposable infusion sets. It is a product that is easily available on the market and can be easily assembled by manufacturers.

[0042] Since the density of air is lower than that of liquid biological samples, the air tends to rise. In one embodiment, a conical portion 11 is provided on one side of the bag body 1 close to the conduit 2, and the conduit 2 is connected to a vertex of the conical portion 11. When the freezing bag is placed vertically, the air will gather at the vertex of the conical portion 11, and the vertex of the conical portion 11 is adjacent to the conduit 2. The path for the air to be discharged from the bag body 1 to the gas collecting chamber 3 becomes shorter, which helps to speed up the exhaust process.

[0043] Specifically, the shape of the cone-like portion 11 can be a cone portion.

[0044] In one embodiment, a valve 4 is further included, in which the catheter 2 is partially placed and arranged, and the valve 4 is arranged on the side of the gas collecting chamber 3 away from the bag body 1; opening or closing the valve 4 to open and close the catheter 2 can prevent the biological sample in the cryopreservation bag from leaking.

[0045] Specifically, the valve 4 is an on-off valve or a flow control valve. The on-off valve can be used to open or close the conduit 2 to reduce the contact time between the biological sample in the cryopreservation bag and the external air; while the flow control valve can be used to control the amount of biological sample added to the cryopreservation bag to ensure that the biological sample in the bag body 1 is not excessive or insufficient. The flow control valve can also control the flow rate of the biological sample to reduce the entrainment of air in the biological sample during the flow process.

[0046] As an example, the flow control valve may be a roller flow rate controller;

[0047] In one embodiment, the bag body 1 has a lowest surface 12 at one end away from the catheter 2, and a pipe mouth is provided on the lowest surface 12. A switch 5 is connected to the pipe mouth (not shown in the figure). When the switch 5 is removed from the pipe mouth, the biological sample flows out of the bag body 1.

[0048] Before taking out the biological sample from the freezing bag, position the freezing bag so that the lowest liquid level of the biological sample in the bag body 1 is on the lowest surface 12, turn on the switch 5, insert the infusion tube into the tube mouth, and then the biological sample can be transferred.

[0049] As an example, the switch 5 can be a rotating plug, an elastic sealing plug, a valve or the like;

[0050] As an example, the switch 5 is a butterfly plug, which includes a plug body 51 that can be inserted into the pipe mouth and two fins 52 connected to both sides of the plug body. By applying force to the two fins 52 and twisting the fins 52 with the axis of the plug body 51 as the rotation center, the butterfly plug can be removed from the bag body 1;

[0051] In some embodiments, the style of the switch 5 may also adopt a butterfly plug as disclosed in the "Three-dimensional multi-chamber cell bag suitable for ultra-low temperature freezing" disclosed in Chinese patent CN215123711U.

[0052] Before putting the biological sample into the freezing bag, the switch 5 can be removed from the bag body 1, and then the biological sample can be poured into the bag body 1. The biological sample fills the tube mouth, and then the switch 5 can be installed on the bag body 1 to avoid the accumulation of bubbles at the tube mouth.

[0053] Specifically, an inverted trapezoidal portion 13 is provided on one side of the bag body 1 near the switch 5. The lowest surface 12 is the short side of the inverted trapezoidal portion 13. Multiple switches 5 may be provided, distributed along the short sides of the inverted trapezoidal portion 13. The provision of multiple switches 5 allows for more thorough drainage of the biological sample from the bag body 1, reducing residual liquid within the biological sample bag 1. It also increases the flow rate of the biological sample, facilitating faster outflow of the biological sample from the bag body 1.

[0054] As an example, the number of the switches 5 can be two, three, or four.

[0055] In one embodiment, the conduit 2 includes a first conduit 21 and a second conduit 22;

[0056] The first conduit 21 is provided between the bag body 1 and the gas collecting chamber 3;

[0057] The second conduit 22 is provided at one end of the gas collecting chamber 3 away from the bag body 1;

[0058] A flow sensor is provided on the first conduit 21, which can be used to sense the flow of the biological sample flowing into the bag body 1 through the first conduit 21; when the bag body 1 is vented, the bag body 1 needs to be squeezed, the capacity of the bag body 1 will become smaller, and there will be liquid in the first conduit 21 and the gas collecting chamber 3; after the air in the bag body 1 is exhausted, the force on the bag body 1 is released, the capacity of the bag body 1 becomes larger, and the biological samples in the first conduit 21 and the gas collecting chamber 3 flow back into the bag body 1. When the flow sensor senses that there is no liquid flowing through the first conduit 21, it means that the biological samples in the gas collecting chamber 3 are emptied and all flow into the bag body 1; for some transparent biological samples whose liquid level is not easy to observe with the naked eye, the setting of the flow sensor helps to remind the operator to perform subsequent operations and remind the operator to seal the cryopreservation bag in time.

[0059] Specifically, the two ends of the first conduit 21 are respectively bonded to the bag body 1 and the gas collecting chamber 3 with a polymer adhesive, and the second conduit 22 is bonded to the gas collecting chamber 3 with a polymer adhesive; the material of the freezing bag is generally made of a polymer organic material with a certain flexibility, and the use of a polymer adhesive can better seal the connection between the first conduit 21, the gas collecting chamber 3 and the second conduit 22 to prevent air from entering and contaminating the biological sample.

[0060] The above is a specific description of the preferred implementation of the present invention, but the invention of the present invention is not limited to the embodiments. Those skilled in the art can make various equivalent modifications or substitutions without violating the spirit of the present invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A freezing bag, characterized in that: include: Bag body (1), catheter (2) and gas collecting chamber (3); The bag (1) is used to store biological samples; The conduit (2) is in communication with the bag body (1), and the conduit (2) is connected to a corner of the bag body (1); The gas collecting chamber (3) is arranged on the conduit (2) and the gas collecting chamber (3) is in communication with the bag body (1) and the conduit (2).

2. The freezing bag according to claim 1, wherein: The cross-sectional area of ​​the gas collecting chamber (3) is larger than the cross-sectional area of ​​the conduit (2).

3. The freezing bag according to claim 2, wherein: The gas collecting chamber (3) is a dripping bucket.

4. The freezing bag according to claim 1, wherein: A conical portion (11) is provided on one side of the bag body (1) close to the conduit (2), and the conduit (2) is connected to a vertex of the conical portion (11).

5. The freezing bag according to claim 1, wherein: It also includes a valve (4), wherein the conduit (2) is partially placed in the valve (4), and the valve (4) is arranged on the side of the gas collecting chamber (3) away from the bag body (1).

6. The freezing bag according to claim 5, wherein: The valve (4) is an on-off valve or a flow control valve.

7. The freezing bag according to claim 1, wherein: The bag body (1) has a lowest surface (12) at one end away from the catheter (2), and a switch (5) is connected to the bag body (1) on the lowest surface (12). The switch (5) is used to allow the biological sample in the bag body (1) to flow out.

8. The freezing bag according to claim 7, wherein: An inverted trapezoidal portion (13) is provided on one side of the bag body (1) close to the switch (5), the lowest surface (12) being the short side of the inverted trapezoidal portion (13), and the switch (5) can be multiple and distributed along the short side of the inverted trapezoidal portion (13).

9. The freezing bag according to claim 1, wherein: The conduit (2) comprises a first conduit (21) and a second conduit (22); The first conduit (21) is provided between the bag body (1) and the gas collecting chamber (3); The second conduit (22) is provided at an end of the gas collecting chamber (3) away from the bag body (1); A flow sensor is provided on the first conduit (21).

10. The freezing bag according to claim 9, wherein: Both ends of the first conduit (21) are respectively bonded to the bag body (1) and the gas collecting chamber (3) via a polymer adhesive, and the second conduit (22) is connected to the gas collecting chamber (3) via the polymer adhesive.

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