Cell clone counting culture plate

Abstract

The utility model discloses a cell clone counting culture plate which comprises a culture plate main body and a counting mechanism, a plurality of culture holes are formed in the culture plate main body; the counting mechanism is arranged at the outer bottom of the culture plate body and comprises a supporting seat, a containing groove is formed in the supporting seat, a counting plate is installed in the containing groove, a plurality of counting grids are arranged on the counting plate and correspond to the culture holes, an adsorption mechanism is installed on the supporting seat, and a plurality of supporting columns are connected to the bottom of the culture plate body. The supporting columns are higher than the supporting seats, and when the counting mechanism is mounted at the outer bottom of the culture plate main body, the culture plate main body is supported by the supporting columns, so that the counting mechanism does not cause deflection of the culture plate main body. The cell clone number in the whole culture plate can be calculated by counting the cell clone number in the specific grids, so that the cell clone counting efficiency can be improved, and the counting error is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell counting, and in particular relates to a cell clone counting culture plate. Background Art

[0002] The colony formation assay is a technique used to assess cell proliferation, invasiveness, and sensitivity to cytotoxic factors. It is commonly used to study cell survival and proliferation potential. By culturing single cells in vitro and observing whether they can form independent clonal colonies, the assay reflects the cell's independent viability and adaptability to its living environment.

[0003] Existing cell clone counting is typically performed using culture plates, which come in 6-well, 12-well, 24-well, 48-well, and 96-well formats. Since cell clones typically number in the dozens to hundreds, counting cells in separate wells of a culture plate can be tedious, time-consuming, and prone to errors.

[0004] Therefore, in response to the above technical problems, it is necessary to provide a cell clone counting culture plate.

[0005] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. Utility Model Content

[0006] The utility model aims to provide a cell clone counting culture plate, which can solve the problem of inconvenient statistics in cell clone counting.

[0007] In order to achieve the above-mentioned purpose, the technical solution provided by a specific embodiment of the present invention is as follows:

[0008] A cell clone counting culture plate comprises: a culture plate body and a counting mechanism;

[0009] A plurality of culture wells are provided in the culture plate body;

[0010] The counting mechanism is arranged at the outer bottom of the culture plate body, and the counting mechanism includes a support seat, a receiving groove is provided in the support seat, a counting plate is installed in the receiving groove, and a plurality of counting grids are provided on the counting plate, and the counting grids correspond to the culture holes. An adsorption mechanism is installed on the support seat.

[0011] In one or more embodiments of the present invention, a plurality of support columns are connected to the bottom of the culture plate body, and the height of the support columns is greater than the height of the support seat. When the counting mechanism is installed on the outer bottom of the culture plate body, the culture plate body will be supported by the plurality of support columns, so that the counting mechanism will not cause the culture plate body to tilt, making it convenient to use the culture plate body to count cell clones.

[0012] In one or more embodiments of the present invention, a leak hole is provided at the center of the bottom of the receiving tank, and the diameter of the leak hole is smaller than the diameter of the counting board, so as to facilitate the removal of the counting board from the receiving tank.

[0013] In one or more embodiments of the present invention, there are four counting grids, each of which is a square. The number of cell clones in each counting grid is first counted, and then the average value is taken, which is the number of clones in the area of ​​the counting grid. The average value is then multiplied by the bottom area of ​​the culture plate body to calculate the number of cell clones in the culture plate body.

[0014] In one or more embodiments of the present invention, a convex ring is provided at the bottom of the accommodating groove, the diameter of the convex ring is larger than the diameter of the leak hole, and a snap-fit ​​groove matching the convex ring is provided on the counting plate. The convex ring is snapped into the snap-fit ​​groove to fix the counting plate, so that the counting plate can be stably placed in the accommodating groove.

[0015] In one or more embodiments of the present invention, a groove is provided on the support seat, the diameter of the groove is larger than the diameter of the convex ring, and when the support seat contacts the bottom of the culture plate body, the groove is in a relatively sealed state.

[0016] In one or more embodiments of the present invention, the adsorption mechanism includes a connecting tube, one end of the connecting tube is arranged in the groove, and the other end of the connecting tube is arranged outside the support seat. When the support seat contacts the bottom of the culture plate body, the groove is connected to the outside world through the connecting tube.

[0017] In one or more embodiments of the present invention, a gas cylinder is threadedly connected to the connecting tube, a piston is slidably connected to the gas cylinder, a screw is rotatably connected to the piston, one end of the screw passes through the gas cylinder, and the screw is threadedly connected to the gas cylinder. By rotating the screw, the screw drives the piston to move in the gas cylinder, and then the gas in the groove can be extracted through the connecting tube, so that the groove forms a negative pressure state, so that the support seat and the bottom of the culture plate body can be firmly fixed.

[0018] In one or more embodiments of the present invention, the convex ring and the piston are both made of rubber.

[0019] In one or more embodiments of the present invention, a sealing gasket is attached to the surface of the support seat to reduce the gap between the culture plate body and the support seat, so that external gas is not easily able to enter the groove, thereby ensuring the adsorption and fixing effect of the culture plate body and the support seat.

[0020] Compared with the prior art, the cell clone counting culture plate of the present invention can calculate the cell clone number in the entire culture plate by counting the cell clone number in a specific square, thereby improving the cell clone counting efficiency and reducing counting errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 This is a three-dimensional diagram of a cell clone counting culture plate in one embodiment of the present invention;

[0023] Figure 2 This is a cross-sectional view of a cell clone counting culture plate in one embodiment of the present invention;

[0024] Figure 3 for Figure 2 Schematic diagram of the structure at A in the middle;

[0025] Figure 4 This is a three-dimensional diagram of a counting mechanism in one embodiment of the present utility model;

[0026] Figure 5 for Figure 4 Schematic diagram of the structure at B in the middle;

[0027] Figure 6 This is a three-dimensional diagram of a culture dish in one embodiment of the present invention.

[0028] Description of main reference numerals:

[0029] 1-culture plate body, 101-support column, 2-counting mechanism, 201-support seat, 202-accommodating groove, 203-counting plate, 204-counting grid, 205-convex ring, 206-groove, 207-connecting tube, 208-air cylinder, 209-piston, 210-screw. DETAILED DESCRIPTION

[0030] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the following will be combined with the drawings of the embodiments of the present invention to clearly and completely describe the technical solutions of the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts should fall within the scope of protection of the present invention.

[0031] Example 1

[0032] like Figures 1 to 5 As shown, a cell clone counting culture plate in one embodiment of the present invention includes a culture plate body 1 and a counting mechanism 2 .

[0033] The culture plate body 1 is provided with a plurality of culture wells for culturing cells.

[0034] Preferably, the number of culture wells is 6, and the bottom area of ​​the culture plate body 1 is 9.6 cm 2 .

[0035] In addition, the bottom of the culture plate body 1 is connected to a plurality of support columns 101, and the height of the support columns 101 is greater than the height of the support base 201. When the counting mechanism 2 is mounted on the outer bottom of the culture plate body 1, the culture plate body 1 is supported by the plurality of support columns 101, and the counting mechanism 2 does not contact the platform. As a result, the counting mechanism 2 does not cause the culture plate body 1 to tilt, making it easier to use the culture plate body 1 to culture cells and count cell clones.

[0036] like Figures 1 to 5 As shown, the counting mechanism 2 is arranged at the outer bottom of the culture plate body 1, and the counting mechanism 2 can play a role in assisting counting.

[0037] The counting mechanism 2 includes a support base 201 , in which a receiving groove 202 is provided. The receiving groove 202 is used to receive a counting plate 203 .

[0038] In addition, a leakage hole is provided at the center of the bottom of the receiving groove 202, and the diameter of the leakage hole is smaller than the diameter of the counting plate 203, which facilitates the removal of the counting plate 203 from the receiving groove 202 so that the counting plate 203 can be replaced according to the different culture plate bodies 1, thereby improving the applicability of the counting mechanism 2.

[0039] Specifically, a counting plate 203 is mounted within the receiving tank 202. The counting plate 203 is provided with a plurality of counting grids 204, each corresponding to a culture well. When counting cells within the culture plate body 1, the cells within the counting grids 204 are first counted, and the average number of cells within the counting grids 204 is calculated. The average number of cells within the culture plate body 1 is then multiplied by the bottom area of ​​the culture plate body 1 to obtain the number of cells within the culture plate body 1. This improves the efficiency of cell clone counting and reduces counting errors.

[0040] Preferably, the counting grid 204 is a 1 cm*1 cm square, and there are four counting grids 204, each of which is a square. The number of cell clones in each counting grid 204 is first counted, and then the average value is taken, which is 1 cm 2 The number of clones in the area is multiplied by the bottom area of ​​the culture plate body 1, which is 9.6 cm 2 , the number of cell clones in the culture plate body 1 can be calculated.

[0041] like Figures 1 to 5 As shown, a convex ring 205 is provided at the bottom of the receiving groove 202, and the diameter of the convex ring 205 is larger than the diameter of the leak hole. A snap-fit ​​groove matching the convex ring 205 is provided on the counting plate 203. By snapping the convex ring 205 into the snap-fit ​​groove, the counting plate 203 can be fixed, so that the counting plate 203 can be stably placed in the receiving groove 202.

[0042] The support seat 201 is provided with a groove 206 , the diameter of the groove 206 is larger than the diameter of the convex ring 205 , and when the support seat 201 contacts the bottom of the culture plate body 1 , the groove 206 is in a relatively sealed state.

[0043] like Figures 1 to 5 As shown, an adsorption mechanism is installed on the support base 201, and the adsorption mechanism can be used to fix the counting mechanism 2 and the bottom of the culture plate body 1.

[0044] The adsorption mechanism includes a connecting tube 207, one end of which is disposed in the groove 206 and the other end of which is disposed outside the support base 201. When the support base 201 contacts the bottom of the culture plate body 1, the groove 206 is connected to the outside through the connecting tube 207.

[0045] In addition, a gas cylinder 208 is threadedly connected to the connecting tube 207, a piston 209 is slidably connected to the gas cylinder 208, and a screw 210 is rotatably connected to the piston 209. One end of the screw 210 passes through the gas cylinder 208, and the screw 210 is threadedly connected to the gas cylinder 208. When the screw 210 is rotated, the screw 210 drives the piston 209 to move in the gas cylinder 208, and then the gas in the groove 206 can be extracted through the connecting tube 207, so that the groove 206 forms a negative pressure state, thereby firmly fixing the support base 201 to the bottom of the culture plate body 1.

[0046] Preferably, the protruding ring 205 and the piston 209 are both made of rubber.

[0047] Specifically, a sealing gasket is attached to the surface of the support seat 201 to reduce the gap between the culture plate body 1 and the support seat 201, so that external gas is not easily able to enter the groove 206, thereby ensuring the adsorption and fixing effect of the culture plate body 1 and the support seat 201.

[0048] In specific use, when it is necessary to count the cells in the culture plate body 1, the counting plate 203 with four counting grids 204 is installed in the receiving groove 202. At this time, the convex ring 205 will be engaged in the engaging groove on the counting plate 203, and the counting plate 203 can be fixed.

[0049] The counting mechanism 2 is placed on the outer bottom of the culture plate body 1, with the support base 201 in contact with the bottom of the culture plate body 1, and the groove 206 is in a relatively sealed state. The screw 210 is rotated, and the screw 210 drives the piston 209 to move within the gas cylinder 208. The gas cylinder 208 can extract gas from the groove 206 through the connecting tube 207, creating a negative pressure in the groove 206. Under the action of the negative pressure, the support base 201 can be adsorbed on the bottom of the culture plate body 1, thereby fixing the counting mechanism 2.

[0050] First count the number of cell clones in each counting grid 204, and then take the average value, which is 1cm 2 The number of cell clones in the main body 1 of the culture plate can be calculated by multiplying the number of clones in the main body 1 of the culture plate by the bottom area of ​​the culture plate.

[0051] When the counting mechanism 2 is not needed, the screw 210 is rotated in the opposite direction, and the gas cylinder 208 injects gas into the groove 206 through the connecting tube 207, thereby reducing the adsorption force between the support seat 201 and the culture plate body 1, making it easier to remove the counting mechanism 2, while also not easily affecting the subsequent photography of the cells in the culture plate body 1.

[0052] Example 2

[0053] The difference from the first embodiment is that Figure 6As shown, the culture plate body 1 is a culture dish, the number of counting grids 204 on the counting plate 203 is five, and the counting grids 204 are 2 cm*2 cm squares. The rest are the same as in Example 1, and the method for counting cells in the culture dish is also the same as in Example 1.

[0054] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed within the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0055] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A cell clone counting culture plate, characterized in that: include: A culture plate body, wherein a plurality of culture wells are provided in the culture plate body; The counting mechanism is arranged at the outer bottom of the culture plate body, and the counting mechanism includes a support base, a receiving groove is provided in the support base, a counting plate is installed in the receiving groove, and a plurality of counting grids are provided on the counting plate, and the counting grids correspond to the culture holes. An adsorption mechanism is installed on the support base.

2. The cell clone counting culture plate according to claim 1, wherein A plurality of support columns are connected to the bottom of the culture plate body, and the height of the support columns is greater than the height of the support seat.

3. The cell clone counting culture plate according to claim 1, wherein A leak hole is provided at the center of the bottom of the accommodating groove, and the diameter of the leak hole is smaller than the diameter of the counting plate.

4. The cell clone counting culture plate according to claim 1, wherein There are four counting squares, and each counting square is a square.

5. The cell clone counting culture plate according to claim 3, characterized in that A convex ring is provided at the bottom of the receiving groove, the diameter of the convex ring is larger than the diameter of the leak hole, and a snap-fit ​​groove matching the convex ring is provided on the counting plate.

6. The cell colony counting culture plate according to claim 5, characterized in that The support seat is provided with a groove, and the diameter of the groove is larger than the diameter of the convex ring.

7. The cell clone counting culture plate according to claim 6, wherein The adsorption mechanism includes a connecting pipe, one end of the connecting pipe is arranged in the groove, and the other end of the connecting pipe is arranged outside the support seat.

8. The cell clone counting culture plate according to claim 7, wherein The connecting pipe is threadedly connected to a gas cylinder, a piston is slidably connected in the gas cylinder, a screw is rotatably connected to the piston, one end of the screw passes through the gas cylinder, and the screw is threadedly connected to the gas cylinder.

9. The cell clone counting culture plate according to claim 8, characterized in that The convex ring and the piston are both made of rubber.

10. The cell colony counting culture plate according to claim 1, characterized in that: A sealing gasket is attached to the surface of the support seat.

Images