Sterile culture device for bacillus subtilis

Abstract

The utility model relates to the technical field of microorganism culture, in particular to a bacillus subtilis group sterile culture device which comprises a cabin body, the cabin body comprises an upper cabin cover and a lower cabin box detachably connected with the upper cabin cover, and the interior of the lower cabin box is rotationally connected with a containing box used for fixing a culture dish box through a driving part. The upper cabin cover is connected with a fixing box through an operating rod, the fixing box is used for being clamped on a culture dish cover, the operating rod penetrates through the upper cabin cover and is connected with the upper cabin cover in a sliding mode, and the lower cabin box is provided with an infrared sterilization mechanism and a containing pipe containing bacterial liquid. The operation steps of sterilizing the inoculating loop, scribing and the like are carried out in the cabin body, so that the pollution of microorganisms in an external environment to an experimental sample is reduced, and the accuracy of a culture experiment result is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial culture, in particular to a device for aseptic culture of Bacillus subtilis groups. Background Art

[0002] Bacillus subtilis is a common Gram-positive bacterium that is widely found in soil, air, plant surfaces and some extreme environments, such as deserts and polar regions. It is a drought-resistant and heat-resistant bacterium that can survive in dry and nutrient-poor environments. Aseptic culture can eliminate the interference of other microorganisms, thereby more accurately studying the growth, metabolism, genetics and other biological characteristics of Bacillus subtilis. And because Bacillus subtilis can produce a variety of enzymes and antibiotics, aseptic culture can improve the purity and yield of these products for use in industrial and medical fields.

[0003] Currently, when culturing Bacillus subtilis, the inoculation loop must first be disinfected, dipped in the bacterial solution, and then streaked on the culture medium. Streaking requires streaking in multiple areas, and the inoculation loop must be disinfected again after each streaking. At the same time, in order to prevent the culture medium from being contaminated, the culture medium cover must also be sealed. This series of operations is not only inefficient, but also frequently opening the culture medium cover can easily expose the culture medium to the external environment, causing contamination. Based on the above situation, we propose a Bacillus subtilis group sterile culture device to solve the above problems. Utility Model Content

[0004] The utility model provides a Bacillus subtilis group aseptic culture device to solve the problems in the prior art of needing to frequently open the culture medium for streaking treatment, low efficiency and easy contamination of the culture medium.

[0005] The technical problem solved by the present invention is achieved by the following technical solutions:

[0006] A Bacillus subtilis group sterile culture device includes a cabin body, the cabin body includes an upper hatch and a lower cabin box detachably connected to the upper hatch, the interior of the lower cabin box is rotatably connected to a containing box for fixing a culture dish box via a driving member, the upper hatch is connected to a fixing box for clamping on the culture dish cover via an operating rod, the operating rod passes through the upper hatch and is slidably connected to the upper hatch, the lower cabin box is provided with an infrared sterilization mechanism and a containing tube containing a bacterial solution, the upper hatch is connected to an inoculation loop via a connecting assembly, and the inoculation loop can be inserted into the infrared sterilization mechanism, the containing tube, and the culture dish box through the connecting assembly to perform sterilization, bacterial solution dipping, and streaking operations;

[0007] It also includes a gas replacement component, which is used to inject pure gas into the cabin to achieve a sterile environment in the cabin.

[0008] Preferably, the gas replacement assembly includes a gas cylinder containing filtered sterile gas, the output end of the gas cylinder is provided with an exhaust pipe extending to the inside of the cabin, the cabin is also connected to an outlet pipe, and valves are connected to the exhaust pipe and the outlet pipe.

[0009] Preferably, the connecting assembly includes a universal ball rotatably connected to the upper hatch and a connecting tube connected to the lower end of the universal ball. The inoculation ring passes through the universal ball and extends to the bottom of the connecting tube. A first spring is connected between the inoculation ring and the lower end of the connecting tube.

[0010] Preferably, elastic friction pads are provided on the inner walls of the containing box and the fixing box, and suction cups are provided inside the containing box and the fixing box.

[0011] Preferably, the lower end of the lower cabin body is rotatably connected to a movable disk, the infrared sterilization mechanism is fixedly connected to the movable disk, and the containing tube is detachably connected to the movable disk.

[0012] Preferably, a protruding ring is provided on the operating rod, a movable plate is rotatably connected to the inner top wall of the upper hatch, and a second spring is provided between the protruding ring and the movable plate.

[0013] The beneficial effects of the present invention are as follows: after the interior of the cabin is replaced with a sterile environment through the gas replacement component, the sterilization and streaking of the inoculation loop and other operation steps are all carried out in the cabin, so as to reduce the contamination of the experimental samples by microorganisms in the external environment, improve the accuracy of the culture experiment results, and complete multiple steps in a specific cabin without the need to frequently change the operating site, saving preparation and transfer time, and also reducing the risk of contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the implementation scheme of the present invention or the technical scheme in the prior art, the drawings required for use in the implementation scheme or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some implementation schemes of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0015] Figure 1 The isometric structural diagram provided for this utility model is:

[0016] Figure 2 The cross-sectional structure diagram provided for the utility model is as follows:

[0017] Figure 3 The utility model provides Figure 2 A magnified schematic diagram of the structure at point A:

[0018] Figure 4 This is a structural diagram of the utility model when the culture dish cover is removed from the fixing box.

[0019] In the figure, 1. cabin body; 101. universal ball; 102. connecting pipe; 103. first spring; 11. upper cabin cover; 12. lower cabin box; 13. movable plate; 2. driving member; 3. culture dish box; 4. container box; 5. operating lever; 51. fixed box; 52. elastic friction pad; 53. suction cup; 54. protruding ring; 55. movable plate; 56. second spring; 6. infrared sterilization mechanism; 7. container tube; 8. inoculating loop; 9. gas cylinder; 91. exhaust pipe; 92. outlet pipe; 93. valve. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below with reference to specific illustrations.

[0021] Reference Figures 1-4 As shown, a Bacillus subtilis group sterile culture device includes a cabin 1, which includes an upper cabin cover 11 and a lower cabin box 12 detachably connected to the upper cabin cover 11. In order to improve the sealing effect, the detachable connection between the upper cabin cover 11 and the lower cabin box 12 is preferably a threaded connection, and the upper cabin cover 11 can be made of a transparent material to facilitate observation of the situation inside the cabin 1. It also includes a gas replacement component, which is used to inject pure gas into the cabin 1 to achieve a sterile environment in the cabin 1. The upper cabin cover 11 is connected to a fixing box 51 for clamping on the culture dish cover through an operating rod 5. The operating rod 5 It passes through the upper hatch 11 and is slidably connected to the upper hatch 11. When in use, the culture dish for marking can be placed in the containing box 4, and then the upper hatch 11 is connected to the lower hatch box 12. The specifications of the containing box 4 and the fixing box 51 can correspond to the specifications of the culture dish. Then, the fixing box 51 is driven to connect with the culture dish cover by the operating rod 5. The fixing box 51 is driven to rotate by rotating the operating rod 5, so that the fixing box 51 removes the culture dish cover, so that the culture dish can be opened in a sterile environment in the cabin 1, thereby avoiding the culture dish from being contaminated by the external environment and affecting the accuracy of the experiment.

[0022] An infrared sterilization mechanism 6 and a container tube 7 containing bacterial liquid are provided on the lower cabin box 12. An inoculation loop 8 is connected to the upper cabin cover 11 through a connecting component. The inoculation loop 8 can be inserted into the infrared sterilization mechanism 6, the container tube 7 and the culture dish box 3 through the connecting component to perform sterilization, bacterial liquid dipping and marking work. Multiple steps can be completed in the sterile environment of the cabin body 1 without frequent changes of the operating site, saving preparation and transfer time, and improving efficiency. At the same time, it also reduces the chance of the inoculation loop 8 dipped in bacterial liquid being exposed to the external environment and easily contaminated. After the inoculation loop 8 performs the marking work on the first area on the culture dish, the driving member 2 can be used to drive the culture dish to rotate a certain angle to facilitate the inoculation loop 8 to perform the marking work on the second area. The driving member 2 can be a servo motor or other device.

[0023] Specifically, the gas replacement component includes a gas cylinder 9 containing filtered sterile gas. The gas in the gas cylinder 9 can be filtered sterile nitrogen, etc. The output end of the gas cylinder 9 is provided with an exhaust pipe 91 extending to the interior of the cabin 1. The cabin 1 is also connected to an outlet pipe 92. Both the exhaust pipe 91 and the outlet pipe 92 are connected to a valve 93. After the cabin 1 is closed, the sterile gas in the gas cylinder 9 is allowed to enter the cabin 1 by opening the valve 93 on the exhaust pipe 91, wherein part of the gas in the cabin 1 can be discharged through the outlet pipe 92. After a certain period of time, the sterile gas discharged through the exhaust pipe 91 fills the cabin 1, and the original gas in the cabin 1 is discharged through the outlet pipe 92, completing the gas replacement, and then closing the valve 93 on the outlet pipe 92. It should be noted that in order to ensure that the gas in the cabin 1 is completely replaced, the approximate time required for gas replacement can be calculated in advance according to the volume of the cabin 1, and a certain amount of surplus can be left in the gas cylinder 9 to ensure sufficient replacement.

[0024] Among them, such as Figure 3 As shown, the connecting assembly includes a universal ball 101 rotatably connected to the upper hatch 11 and a connecting tube 102 connected to the lower end of the universal ball 101. The inoculating loop 8 passes through the universal ball 101 and extends to the bottom of the connecting tube 102. A first spring 103 is connected between the inoculating loop 8 and the lower end of the connecting tube 102. By pressing the inoculating loop 8, the inoculating loop 8 can compress the first spring 103 and move downward, so that the inoculating loop 8 enters the infrared sterilization mechanism 6 for disinfection or dips into the holding tube 7 to obtain bacterial solution. The universal ball 101 can drive the inoculating loop 8 to swing freely, so that the end of the inoculating loop 8 can be moved to the culture dish box 3 for marking. The infrared sterilization mechanism 6 adopts the principle of infrared heating, which can make the inoculating loop 8 reach a high-temperature sterilization state within a few seconds. This technology is existing technology and should be known to those skilled in the art. No further details will be given here.

[0025] Further, such as Figure 2 as well as Figure 4As shown, in order to fix the culture dish cover and the culture dish box 3, elastic friction pads 52 are provided on the inner walls of the containing box 4 and the fixing box 51. The elastic friction pads 52 can be made of rubber, silicone or other materials, and suction cups 53 are provided inside the containing box 4 and the fixing box 51. While the elastic friction pads 52 increase the friction with the culture dish cover and the culture dish box 3, the suction cups 53 can be used to fix the culture dish cover and the culture dish box 3, so that when the fixing box 51 rotates, the culture dish box 3 will not be driven to rotate synchronously, thereby facilitating the removal of the culture dish cover threadedly connected to the culture dish box 3.

[0026] Further, such as Figure 2 As shown, the operating rod 5 is provided with a protruding ring 54, and a movable plate 55 is rotatably connected to the inner top wall of the upper hatch 11, and a second spring 56 is provided between the protruding ring 54 and the movable plate 55. When the operating rod 5 is rotated, the movable plate 55 can be driven to rotate synchronously by the second spring 56. When the culture dish cover needs to be removed, the operating rod 5 is first pressed downward to drive the fixed box 51 to connect with the culture dish cover, and then the operating rod 5 is rotated to remove it. Thereafter, the fixed box 51 and the culture dish cover are driven upward by the action of the second spring 56 to prevent the culture dish cover from affecting the marking work of the inoculation loop 8.

[0027] Among them, such as Figure 2 As shown, the lower end of the lower cabin body 1 is rotatably connected to a movable disk 13, the infrared sterilization mechanism 6 is fixedly connected to the movable disk 13, and the containing tube 7 is detachably connected to the movable disk 13. When in use, the movable disk 13 can be rotated to drive the infrared sterilization mechanism 6 or the containing tube 7 to be located directly below the inoculation ring 8. The inoculation ring 8 is pressed downward to make the inoculation ring 8 move downward and extend into the infrared sterilization mechanism 6 or the containing tube 7, so that the bacteria can be dipped or disinfected and sterilized. There is a certain friction between the movable disk 13 and the lower cabin body 1, and it will not rotate at will. Only when the rotational force applied to the movable disk 13 is greater than the friction between the movable disk 13 and the lower cabin body 1, the movable disk 13 will rotate. Moreover, when the gas replacement in the cabin body 1 is completed, the containing tube 7 can be installed on the movable disk 13 to prevent the bacteria in the containing tube 7 from being contaminated by the outside world.

[0028] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A sterile culture device for Bacillus subtilis, characterized in that: The invention comprises a cabin body (1), wherein the cabin body (1) comprises an upper cabin cover (11) and a lower cabin box (12) detachably connected to the upper cabin cover (11); the interior of the lower cabin box (12) is rotatably connected to a containing box (4) for fixing a culture dish box (3) through a driving member (2); the upper cabin cover (11) is connected to a fixing box (51) for being clamped on the culture dish cover through an operating rod (5); the operating rod (5) passes through the upper cabin cover (11) and is slidably connected to the upper cabin cover (11); the lower cabin box (12) is provided with an infrared sterilization mechanism (6) and a containing tube (7) containing bacterial liquid; the upper cabin cover (11) is connected to an inoculation loop (8) through a connecting assembly, and the inoculation loop (8) can be inserted into the infrared sterilization mechanism (6), the containing tube (7) and the culture dish box (3) through the connecting assembly to perform sterilization, bacterial liquid dipping and marking operations; It also includes a gas replacement component, which is used to inject pure gas into the cabin (1) to achieve a sterile environment in the cabin (1).

2. The aseptic culture device for Bacillus subtilis according to claim 1, characterized in that: The gas replacement assembly comprises a gas cylinder (9) containing filtered sterile gas, an output end of the gas cylinder (9) being provided with an exhaust pipe (91) extending into the interior of the cabin (1), an outlet pipe (92) being further connected to the cabin (1), and valves (93) being connected to both the exhaust pipe (91) and the outlet pipe (92).

3. The aseptic culture device for Bacillus subtilis according to claim 1, characterized in that: The connecting assembly includes a universal ball (101) rotatably connected to the upper hatch (11) and a connecting tube (102) connected to the lower end of the universal ball (101); the inoculation ring (8) passes through the universal ball (101) and extends to the bottom of the connecting tube (102); a first spring (103) is connected between the inoculation ring (8) and the lower end of the connecting tube (102).

4. The aseptic culture device for Bacillus subtilis according to claim 1, characterized in that: Elastic friction pads (52) are provided on the inner walls of the containing box (4) and the fixing box (51), and suction cups (53) are provided inside the containing box (4) and the fixing box (51).

5. The aseptic culture device for Bacillus subtilis according to claim 1, characterized in that: The lower end of the lower cabin (1) is rotatably connected to a movable disk (13), the infrared sterilization mechanism (6) is fixedly connected to the movable disk (13), and the containing tube (7) is detachably connected to the movable disk (13).

6. The aseptic culture device for Bacillus subtilis according to claim 1, characterized in that: A protruding ring (54) is provided on the operating rod (5), a movable plate (55) is rotatably connected to the inner top wall of the upper hatch (11), and a second spring (56) is provided between the protruding ring (54) and the movable plate (55).

Images