Efficient single cell separator

Abstract

The utility model discloses an efficient single cell separator which comprises a mounting base, a test tube storage rack and a heat preservation shell are fixedly mounted at the top of the mounting base, the heat preservation shell is located on the right side of the test tube storage rack, a rotating rack is rotationally connected to the inner wall of the heat preservation shell, and a centrifugal seat is fixedly mounted at the top of the rotating rack; a centrifugal motor is fixedly installed on the inner bottom wall of the heat preservation shell, and an output shaft of the centrifugal motor is fixedly installed at the bottom of the rotating frame. According to the utility model, the separation test tube is placed in the bearing cylinder, the electromagnetic valve is used for controlling the gas inlet rate, and the temperature sensor is matched to realize automatic temperature control, so that the simultaneous separation operation of single cell solutions with different temperature requirements is realized, the separation efficiency is improved, and the operation steps of personnel are reduced; meanwhile, by arranging the auxiliary assembly, multiple groups of separation test tubes can slowly rotate before centrifugal operation, so that the single-cell solution is uniformly dispersed, and the influence of agglomerates on subsequent separation is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation, in particular to a high-efficiency single cell separator. Background Art

[0002] A single-cell isolator is an instrument specifically designed to accurately and rapidly isolate single cells from biological samples. This device is crucial in the fields of biotechnology, medical research, drug development, and clinical diagnostics because it can provide high-quality single-cell samples for further analysis and research.

[0003] In daily life, single cells usually need to be separated before they can be tested and detected. However, existing single-cell separation methods usually use micromanipulation to separate single cells. The solution containing the single cells must be centrifuged beforehand. However, cell centrifugation usually requires a one-to-one operation to complete, which is complicated and not conducive to the rapid completion of the single-cell solution separation operation, affecting daily work efficiency.

[0004] After searching, the patent with application number CN2020230097153 discloses a rapid single-cell separation device, including a workbench, a placement table, a mounting seat and a mounting frame, wherein a separation frame is provided inside the mounting frame, the bottom of the separation frame is fixedly connected to a rotating rod, one end of the rotating rod is fixedly connected to a motor assembly, the surface of the separation frame is provided with a fixed groove, and the interior of the fixed groove is movably connected to a separation tube. In the present utility model, the separation frame, the separation tube and the separation net provided can realize rapid centrifugal separation of different single-cell organisms in different single-cell solutions in multiple separation tubes. When in use, the staff first takes out the separation tube placed in the placement hole on the placement table, pours the single-cell solution into the separation net in the separation tube, and then places the separation tube containing the single-cell solution in the fixed groove in the separation frame, and uses the fixed clamp on the separation tube to quickly clamp the separation tube.

[0005] This structure performs synchronous cell separation by installing multiple groups of separation tubes on a separation rack. However, the structure lacks a temperature control device. Cell separation requires a certain low temperature condition, and different single-cell solutions require different temperature environments. In actual operation, multiple operations are required, which affects the separation efficiency. At the same time, the cell solution needs to be shaken before placement to prevent clumping. Shaking multiple groups of test tubes in sequence increases the workload of personnel and reduces the separation efficiency. Utility Model Content

[0006] In response to the problems existing in the prior art, the purpose of the present invention is to provide a high-efficiency single-cell separation instrument, which improves the separation efficiency by placing the separation test tube inside the carrying cylinder, controlling the air intake rate by the solenoid valve, and cooperating with a temperature sensor to achieve automatic temperature control.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] The top of the centrifugal seat is provided with a centrifugal seat, and the inner bottom wall of the centrifugal seat is fixedly mounted with a centrifugal motor. The output shaft of the centrifugal motor is fixedly mounted on the bottom of the rotating frame. The top of the centrifugal seat is provided with evenly distributed through grooves. The inner wall of the through groove is rotatably connected with a carrying cylinder. The bottom end of the carrying cylinder passes through the through groove and extends to the bottom of the centrifugal seat. The top of the carrying cylinder is provided with a separation test tube, the bottom end of the separation test tube is located inside the carrying cylinder, an electromagnetic valve is installed at the bottom of the carrying cylinder, and a temperature sensor is fixedly mounted on the inner wall of the carrying cylinder. A refrigerator is installed on the right side of the thermal insulation shell. A processor and a signal transmission module are integrated in the interior of the thermal insulation shell. The signal transmission module, the refrigerator, the electromagnetic valve and the temperature sensor are all connected to the processor signal. Auxiliary components are provided inside the thermal insulation shell.

[0009] As a further description of the above technical solution: a control panel is fixedly installed on the top of the mounting base, and the centrifugal motor, refrigerator, solenoid valve and auxiliary components are all electrically connected to the control panel.

[0010] As a further description of the above technical solution: a flashing light is installed on the outside of the heat-insulating shell, and the flashing light is connected to the processor signal.

[0011] As a further description of the above technical solution: the auxiliary component includes a synchronous motor, which is fixedly installed at the bottom of the centrifugal seat, and a synchronous gear disc is fixedly connected to the output shaft of the synchronous motor, and an outer gear ring is fixedly connected to the outer side of the supporting cylinder, and the outer gear ring is located on the outer side of the synchronous gear disc and meshes with the synchronous gear disc.

[0012] As a further description of the above technical solution: a metal ring is fixedly connected to the outer side of the separation tube, a magnetic ring is connected to the top of the carrying tube, and the metal ring is located on the top of the magnetic ring and is magnetically connected to the magnetic ring.

[0013] As a further description of the above technical solution: a heat-insulating cover is hingedly connected to the top of the heat-insulating shell, and a handle is connected to the top of the heat-insulating cover.

[0014] Compared with the prior art, the advantages of the present invention are:

[0015] In the utility model, the separation test tubes are placed inside the carrier tube, the air intake rate is controlled by the solenoid valve, and the temperature sensor is used to realize automatic temperature control, thereby realizing the simultaneous separation operation of single-cell solutions with different temperature requirements, improving the separation efficiency and reducing the number of human operation steps. At the same time, by setting up auxiliary components, multiple groups of separation test tubes can be rotated slowly before centrifugation, so that the single-cell solution is evenly dispersed to avoid the formation of clumps that affect subsequent separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the cross-sectional structure of the thermal insulation shell of the present invention;

[0018] Figure 3 This is a schematic diagram of the cross-sectional structure of the support tube of the present utility model;

[0019] Figure 4 It is a schematic diagram of the principle of the present utility model.

[0020] Description of the numbers in the figure:

[0021] 1. Mounting base; 2. Test tube rack; 3. Insulation shell; 4. Rotating rack; 5. Centrifugal seat; 6. Centrifugal motor; 7. Through slot; 8. Carrying cylinder; 9. Separation test tube; 10. Solenoid valve; 11. Temperature sensor; 12. Refrigerator; 13. Processor; 14. Auxiliary components; 1401. Synchronous motor; 1402. Synchronous gear disc; 1403. Outer gear ring; 15. Control panel; 16. Flashing light; 17. Metal ring; 18. Magnetic ring; 19. Insulation cover. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0023] See also Figures 1 to 4In the present invention, a high-efficiency single cell separation instrument comprises a mounting base 1, a test tube rack 2 and a heat-insulating shell 3 are fixedly mounted on the top of the mounting base 1, the heat-insulating shell 3 is located on the right side of the test tube rack 2, a rotating frame 4 is rotatably connected to the inner wall of the heat-insulating shell 3, a centrifugal seat 5 is fixedly mounted on the top of the rotating frame 4, a centrifugal motor 6 is fixedly mounted on the inner bottom wall of the heat-insulating shell 3, the output shaft of the centrifugal motor 6 is fixedly mounted on the bottom of the rotating frame 4, the top of the centrifugal seat 5 is provided with evenly distributed through grooves 7, a carrying cylinder 8 is rotatably connected to the inner wall of the through groove 7, and the carrying cylinder The bottom end of 8 passes through the through groove 7 and extends to the bottom of the centrifugal seat 5. A separation test tube 9 is installed on the top of the carrying cylinder 8. The bottom end of the separation test tube 9 is located inside the carrying cylinder 8. A solenoid valve 10 is installed at the bottom of the carrying cylinder 8. A temperature sensor 11 is fixedly installed on the inner wall of the carrying cylinder 8. A refrigerator 12 is installed on the right side of the thermal insulation shell 3. A processor 13 and a signal transmission module are integrated inside the thermal insulation shell 3. The signal transmission module, refrigerator 12, solenoid valve 10 and temperature sensor 11 are all connected to the processor 13 signal. An auxiliary component 14 is provided inside the thermal insulation shell 3.

[0024] The auxiliary component 14 includes a synchronous motor 1401, which is fixedly installed at the bottom of the centrifugal seat 5. A synchronous gear disc 1402 is fixedly connected to the output shaft of the synchronous motor 1401, and an outer gear ring 1403 is fixedly connected to the outer side of the supporting tube 8. The outer gear ring 1403 is located on the outer side of the synchronous gear disc 1402 and engages with the synchronous gear disc 1402.

[0025] A metal ring 17 is fixedly connected to the outside of the separation tube 9 , and a magnetic ring 18 is connected to the top of the carrying cylinder 8 . The metal ring 17 is located on the top of the magnetic ring 18 and is magnetically connected to the magnetic ring 18 .

[0026] A heat-insulating cover plate 19 is hinged on the top of the heat-insulating outer shell 3 , and a handle is connected to the top of the heat-insulating cover plate 19 .

[0027] When single cells need to be separated, the test tube rack 2 can be used to place the test tubes. After taking out the separation test tube 9, the single cell solution is poured into the separation test tube 9. There is a separation net inside the separation test tube 9. The user then inserts the separation test tube 9 into the through slot 7 so that its bottom end enters the interior of the carrying tube 8. During the downward movement, the metal ring 17 on the side contacts the magnetic ring 18 and is adsorbed on its top, thereby completing the limitation of the separation test tube 9. The user then inserts the separation test tube 9 into different carrying tubes 8 in turn. The user then closes the top insulation cover 19 to make the insulation shell 3 sealed. The user then controls the operation of the auxiliary component 14, and the synchronous motor 1401 drives the synchronous gear disc 1402 fixed to it to rotate slowly. The outer gear ring 1403 evenly distributed on the side begins to rotate accordingly. Multiple groups of carrying tubes 8 drive multiple groups of separation test tubes 9 to rotate, so that the inner The single cell solution is shaken to avoid cell clumping. Then the user controls the opening degree of the solenoid valve 10 according to the temperature required by the cell solution in each separation tube 9, so that the air intake rate changes. The internal temperature sensor 11 monitors the temperature value in real time and transmits it to the processor 13. The refrigerator 12 operates to fill cold air into the interior of the thermal insulation shell 3. The cold air enters the interior of the carrier tube 8 through the solenoid valve 10, so that the temperature of the separation tube 9 and the cell liquid drops and remains constant. The processor 13 changes the opening and closing state of the solenoid valve 10 in real time to keep the internal temperature of the carrier tube 8 within the set range. The centrifugal motor 6 drives the rotating frame 4 to rotate, and the centrifugal seat 5 rotates accordingly, cooperating with the auxiliary component 14 to achieve rapid separation of the single cell solution, isolating the single cell in the separation net. When the separation is completed, the user controls the electrical appliance to stop and lifts the thermal insulation cover 19 to take out the separation tube 9.

[0028] In the present invention, the separation test tubes 9 are placed inside the carrier tube 8, the air intake rate is controlled by the solenoid valve 10, and the temperature sensor 11 is used to realize automatic temperature control, thereby realizing the simultaneous separation operation of single-cell solutions with different temperature requirements, improving the separation efficiency and reducing the number of human operation steps. At the same time, by setting the auxiliary component 14, multiple groups of separation test tubes 9 can be rotated slowly before centrifugation, so that the single-cell solution is evenly dispersed to avoid the formation of clumps that affect subsequent separation.

[0029] See also Figure 1 , wherein: a control panel 15 is fixedly mounted on the top of the mounting base 1 , and the centrifugal motor 6 , the refrigerator 12 , the solenoid valve 10 and the auxiliary components 14 are all electrically connected to the control panel 15 .

[0030] In the present invention, the setting of the control panel 15 allows users to operate the device more simply and quickly, thereby reducing the steps required by the users and improving the efficiency of single cell separation.

[0031] See also Figure 1 and 4, wherein: a flashing light 16 is installed on the outside of the heat-insulating shell 3 , and the flashing light 16 is connected to the processor 13 signal.

[0032] In the present invention, when there is an abnormality in the temperature data, the processor 13 will control the flashing light 16 to flash, which can prompt nearby personnel so that they can repair the equipment in time.

[0033] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any person skilled in the art who, within the technical scope disclosed in the present invention, makes equivalent substitutions or modifications based on the technical solutions and improved concepts of the present invention shall be covered by the scope of protection of the present invention.

Claims

1. An efficient single cell separation instrument, comprising a mounting base (1), characterized in that: A test tube rack (2) and a heat-insulating shell (3) are fixedly mounted on the top of the mounting base (1), the heat-insulating shell (3) is located on the right side of the test tube rack (2), a rotating rack (4) is rotatably connected to the inner wall of the heat-insulating shell (3), a centrifugal seat (5) is fixedly mounted on the top of the rotating rack (4), a centrifugal motor (6) is fixedly mounted on the inner bottom wall of the heat-insulating shell (3), an output shaft of the centrifugal motor (6) is fixedly mounted on the bottom of the rotating rack (4), the top of the centrifugal seat (5) is provided with evenly distributed through grooves (7), a bearing cylinder (8) is rotatably connected to the inner wall of the through groove (7), the bottom end of the bearing cylinder (8) passes through the through groove (7) and extends to The bottom of the centrifugal seat (5) is provided with a separation test tube (9) installed on the top of the carrying cylinder (8), the bottom end of the separation test tube (9) is located inside the carrying cylinder (8), the bottom of the carrying cylinder (8) is provided with a solenoid valve (10), the inner wall of the carrying cylinder (8) is fixedly provided with a temperature sensor (11), a refrigerator (12) is provided on the right side of the heat-insulating shell (3), a processor (13) and a signal transmission module are integrated inside the heat-insulating shell (3), the signal transmission module, the refrigerator (12), the solenoid valve (10) and the temperature sensor (11) are all connected to the processor (13) by signal, and an auxiliary component (14) is provided inside the heat-insulating shell (3).

2. The high-efficiency single cell separation instrument according to claim 1, characterized in that: A control panel (15) is fixedly mounted on the top of the mounting base (1), and the centrifugal motor (6), refrigerator (12), solenoid valve (10) and auxiliary components (14) are all electrically connected to the control panel (15).

3. The high-efficiency single cell separation instrument according to claim 1, characterized in that: A flashing light (16) is installed on the outside of the heat-insulating shell (3), and the flashing light (16) is connected to the processor (13) by signal.

4. The high-efficiency single cell separation instrument according to claim 1, characterized in that: The auxiliary component (14) includes a synchronous motor (1401), which is fixedly mounted on the bottom of the centrifugal seat (5). A synchronous toothed disc (1402) is fixedly connected to the output shaft of the synchronous motor (1401). An outer toothed ring (1403) is fixedly connected to the outer side of the supporting cylinder (8). The outer toothed ring (1403) is located on the outer side of the synchronous toothed disc (1402) and meshes with the synchronous toothed disc (1402).

5. The high-efficiency single cell separation instrument according to claim 1, characterized in that: A metal ring (17) is fixedly connected to the outside of the separation test tube (9), a magnetic ring (18) is connected to the top of the supporting tube (8), and the metal ring (17) is located on the top of the magnetic ring (18) and is magnetically connected to the magnetic ring (18).

6. The high-efficiency single cell separation instrument according to claim 1, characterized in that: A heat-insulating cover plate (19) is hingedly connected to the top of the heat-insulating shell (3), and a handle is connected to the top of the heat-insulating cover plate (19).

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