Nutrient solution adding tool for cell culture medium

Abstract

The utility model discloses a nutrient solution adding tool for a cell culture medium, which comprises a base and further comprises a base, a plurality of connecting rods and a plurality of connecting rods, the culture disc is provided with a connecting part matched with the base, and a plurality of placing grooves are formed in the culture disc in the circumferential direction and used for placing culture dishes. According to the nutrient solution adding tool for the cell culture medium provided by the utility model, when a plurality of culture dishes on the culture tray need to be subjected to solution change, the culture tray is connected with the substrate on the base, and when the solution change is finished, the culture dish on the culture tray is connected with the substrate on the base; according to the device, a plurality of culture dishes are placed on the culture disc for centralized culture, when a nutrient solution is replaced, the plurality of culture dishes can be taken out at a time, and the solution of the plurality of culture dishes can be replaced in a rotating mode, so that the operation is convenient and fast, and the operation efficiency is improved. And the liquid changing time in the whole process is shortened.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a nutrient solution adding tool for cell culture medium. Background Art

[0002] As is known to all, cell culture refers to the process of removing cells from animals or plants and then growing them in a suitable artificial environment. Cells can be directly removed from tissues before culture and dissociated by enzymatic or mechanical methods. The cells are placed in a culture dish and culture medium is used to provide the cells with necessary nutrients, gases, growth factors, etc.

[0003] During the cell culture process, if the cell culture medium turns yellow, the cell culture medium can be changed after eliminating contamination. The specific steps are to transfer the cell culture flask that needs to be changed to an ultra-clean workbench, remove the old culture medium, add preheated fresh complete culture medium, and place the cells after the change of culture medium in a 37°C carbon dioxide incubator for culture.

[0004] During the above-mentioned cell culture process, when it is observed that the nutrient solution of multiple culture dishes needs to be replaced, the operator takes out the culture dishes one by one and places them on the workbench, then replaces the nutrient solution and puts them back into the incubator and takes out the next culture dish that needs to be replaced. This method of replacing the nutrient solution of the culture dishes one by one takes a long time, and the overall process is cumbersome and inconvenient. Utility Model Content

[0005] In view of the above-mentioned problems existing in the prior art, one aspect of the present invention is to provide a nutrient solution adding tool for cell culture medium to solve the above-mentioned deficiencies in the prior art.

[0006] In order to achieve the above-mentioned purpose, the utility model provides a nutrient solution adding tool for cell culture medium, comprising a base, and also comprising: a base, which is rotatably arranged on the base; a culture tray, which is provided with a connecting portion that cooperates with the base, and the culture tray is provided with multiple placement grooves along the circumferential direction, which are used to place culture dishes.

[0007] Preferably, each placement slot is provided with a notch portion.

[0008] Preferably, each of the notches is in a rectangular structure as a whole, and an inclined portion is provided on the notch.

[0009] Preferably, each of the culture dishes consists of a dish body and a dish cover.

[0010] Preferably, a rotating block is provided in the rotating groove provided on the culture tray.

[0011] Preferably, the rotating block is movably connected to a pressure plate via an elastic reset member.

[0012] Preferably, a limiting portion is provided at the top center of the culture dish.

[0013] Preferably, the pressure plate is assembled to have the following two working states: a first working state: when the pressure plate and the limiting portion do not overlap, the elastic reset member is stretched; a second working state: when the pressure plate and the limiting portion overlap, the elastic reset member contracts so that the pressure plate abuts against each of the culture dishes.

[0014] In the above technical solution, the utility model provides a nutrient solution adding tool for cell culture medium, which has the following beneficial effects: the utility model places multiple culture dishes in the placement groove on the culture tray, and then places the culture tray in the incubator. When the multiple culture dishes on the culture tray need to be replaced, the culture tray is taken out of the incubator and then connected to the base on the base for installation. When the liquid is replaced, after the liquid is replaced for one culture dish on the culture tray, the next culture dish to be replaced is turned in front of the operator by rotating the culture tray, which is convenient for replacement. After all culture dishes are replaced, the culture tray is placed in the incubator. The device places multiple culture dishes on the culture tray for centralized culture. When the nutrient solution is replaced, multiple culture dishes can be taken out at one time and the liquid can be replaced for multiple culture dishes by rotating. The operation is convenient, the liquid replacement time of the entire process is reduced, and even the operator is prevented from taking out the culture dishes one by one and placing them on the workbench, then putting them back into the incubator after replacing the nutrient solution and taking out the next culture dish to be replaced. The method of replacing the nutrient solution of the culture dishes one by one takes a long time, and the overall process operation is cumbersome and inconvenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0016] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 This is a partially enlarged structural diagram of the pressing plate of the utility model when it is implemented;

[0018] Figure 3 This is a schematic diagram of the explosion structure of the base and culture tray of the utility model;

[0019] Figure 4 This is a schematic diagram of the base structure of the utility model;

[0020] Figure 5This is a schematic diagram of the partial structure of the internal section of the culture tray and the pressing plate of the utility model;

[0021] Figure 6 It is a partially enlarged structural diagram of the limiting part of the utility model.

[0022] Description of reference numerals:

[0023] 1. Base; 2. Culture tray; 3. Pressing plate; 4. Culture dish; 1.1. Base; 2.1. Placement slot; 2.2. Limiting portion; 2.3. Wedge-shaped portion; 2.4. Rotation slot; 2.5. Connecting portion; 2.6. Notch portion; 2.7. Inclined portion; 3.1. Elastic reset member; 3.2. Rotation block; 4.1. Tray body; 4.2. Tray cover. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0025] See also Figure 1-6 The cell culture medium nutrient solution adding tool is used to solve the problem that when multiple culture dishes need to have their nutrient solution replaced during cell culture, the operator takes out the culture dishes one by one and places them on the workbench, then replaces the nutrient solution and puts them back into the incubator and takes out the next culture dish that needs to be replaced. The method of replacing the nutrient solution of the culture dishes one by one takes a long time, and the overall process is cumbersome and inconvenient.

[0026] As a further technical solution proposed by the present invention, it includes a base 1 and also includes: a base 1.1, which is rotatably arranged on the base 1; a culture tray 2, which is provided with a connecting portion 2.5 that cooperates with the base 1.1, and the two are snap-fitted to complete the snap-fit ​​installation of the base 1 and the culture tray 2, so that the culture tray 2 can rotate on the base 1 through the rotating base 1.1. A plurality of placement grooves 2.1 are provided on the culture tray 2 along the circumferential direction, which are used to place culture dishes 4. Specifically, after placing a plurality of culture dishes 4 in the placement grooves 2.1 on the culture tray 2, the culture tray 2 is placed in an incubator. When the plurality of culture dishes 4 on the culture tray 2 need to be replaced with liquid, the culture tray 2 is taken out of the incubator, and then the culture tray 2 is connected and installed with the base 1.1 on the base 1. When the culture dish 4 on the culture tray 2 is replaced, the culture dish 4 to be replaced is turned in front of the operator by rotating the culture tray 2 to facilitate replacement. After all the culture dishes are replaced, the culture tray 2 is placed in the incubator. The device places multiple culture dishes on the culture tray 2 for centralized culture. When the nutrient solution is replaced, multiple culture dishes can be taken out at one time and the solution can be replaced in sequence by rotating the multiple culture dishes. The operation is convenient, which reduces the time of the entire process of changing the solution, reduces or even avoids the operator taking out the culture dishes and placing them on the workbench in turn, and then putting them back into the incubator after replacing the nutrient solution and taking out the next culture dish to be replaced. The method of replacing the nutrient solution of the culture dishes in turn takes a long time, and the overall process operation is cumbersome and inconvenient.

[0027] The number of placement slots 2.1 in this embodiment is set according to the needs of those skilled in the art, that is, according to the number of cells to be cultured therein. Preferably, the specific number of placement slots 2.1 is three, such as Figure 1 As shown in the state, three placement slots 2.1 are correspondingly placed with three culture dishes 4 for centralized culture.

[0028] In another embodiment of the present invention, preferably, each placement slot 2.1 is provided with a notch portion 2.6. Furthermore, the notch portion 2.6 is used to facilitate the operator to insert a finger into the placement slot 2.1 to take out the culture dish 4 or open the dish cover 4.2 on the culture dish 4.

[0029] In another embodiment of the present invention, preferably, each notch portion 2.6 is a rectangular structure as a whole, and a slope portion 2.7 is provided on the notch portion 2.6. Furthermore, the inclined slope portion 2.7 is more convenient for the operator to insert his fingers into the placement slot 2.1. Figure 2 Status shown.

[0030] In another embodiment of the present invention, each culture dish 4 is specifically composed of a dish body 4.1 and a dish cover 4.2. Figure 1As shown in the state, the culture dish 4 consists of a dish body 4.1 and a dish cover 4.2. The cells are placed in the dish body 4.1, and the dish cover 4.2 is used to be snap-fitted with the dish body 4.1 for installation and connection. This is existing technology and will not be described in detail.

[0031] In another embodiment of the present invention, preferably, a rotating block 3.2 is provided in the rotating groove 2.4 opened on the culture tray 2, and the rotating block 3.2 is movably connected to the pressing plate 3 through the elastic reset member 3.1. Further, as Figure 5 As shown in the state, the pressing plate 3 is slidably connected to the rotating block 3.2, and an elastic reset member 3.1 is connected between the two. The pressing plate 3 rotates synchronously with the rotating block 3.2 on the culture tray 2, and the elastic reset member 3.1 is specifically a spring.

[0032] In another embodiment of the present invention, a limiting portion 2.2 is provided at the top center of the culture dish 2. The limiting portion 2.2 is a triangular structure as a whole, which is adapted to the pressing plate 3. The pressing plate 3 is assembled to have the following two working states: a first working state: when the pressing plate 3 and the limiting portion 2.2 do not overlap, the elastic reset member 3.1 is stretched; a second working state: when the pressing plate 3 and the limiting portion 2.2 overlap, the elastic reset member 3.1 contracts so that the pressing plate 3 abuts against each culture dish 4. Specifically, the first working state of the pressing plate 3 is as follows: Figure 1 As shown in the state, the pressing plate 3 does not overlap with the limiting portion 2.2, so that the spring is stretched. This state is used by the staff to change the liquid of the culture dish 4 on the culture tray 2, and the second working state of the pressing plate 3 is as shown in the state Figure 2 As shown in the state, the pressing plate 3 coincides with the limiting portion 2.2, so that the pressing plate 3 sinks into the limiting portion 2.2. During the sinking process, the spring contracts so that the pressing plate 3 abuts and fixes the culture dish 4 that has been exchanged, thereby improving the stability of the culture dish 4 in the placement groove 2.1 when the staff takes the mobile culture dish 2. Furthermore, a wedge-shaped portion 2.3 is provided on the limiting portion 2.2, and the wedge-shaped portion 2.3 and the pressing plate 3 can be wedge-fitted. When the pressing plate 3 coincides with the limiting portion 2.2, the wedge-shaped cooperation with the wedge-shaped portion 2.3 under rotation facilitates the pressing plate 3 to rotate out of the limiting portion 2.2 and then to be Figure 1 status.

[0033] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A nutrient solution adding tool for cell culture medium, characterized in that: It comprises a base (1), and further comprises: a base (1.1) rotatably mounted on the base (1); A culture tray (2) is provided with a connecting portion (2.5) that matches the base (1.1). The culture tray (2) is provided with a plurality of placement grooves (2.1) along a circumferential direction for placing culture dishes (4).

2. The nutrient solution adding tool for cell culture medium according to claim 1, characterized in that: Each placement slot (2.1) is provided with a slot portion (2.6).

3. The nutrient solution adding tool for cell culture medium according to claim 2, characterized in that: Each notch portion (2.6) is of a rectangular structure as a whole, and an inclined portion (2.7) is provided on the notch portion (2.6).

4. The nutrient solution adding tool for cell culture medium according to claim 1, characterized in that: Each of the culture dishes (4) is specifically composed of a dish body (4.1) and a dish cover (4.2).

5. The nutrient solution adding tool for cell culture medium according to claim 1, characterized in that: A rotating block (3.2) is provided in the rotating groove (2.4) provided on the culture tray (2).

6. The nutrient solution adding tool for cell culture medium according to claim 5, characterized in that: The rotating block (3.2) is movably connected to a pressing plate (3) via an elastic reset member (3.1).

7. The nutrient solution adding tool for cell culture medium according to claim 6, characterized in that: A limiting portion (2.2) is provided at the top center of the culture tray (2).

8. The nutrient solution adding tool for cell culture medium according to claim 7, characterized in that: The pressing plate (3) is assembled to have the following two working states: a first working state: when the pressing plate (3) and the limiting portion (2.2) do not overlap, the elastic reset member (3.1) is stretched; a second working state: when the pressing plate (3) and the limiting portion (2.2) overlap, the elastic reset member (3.1) contracts so that the pressing plate (3) abuts against each of the culture dishes (4).

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