Double-sided adjustable cell co-culture device

By designing a rotary device in a cell co-culture device to regulate the up and down flip of the diaphragm, the problems of complex and contamination risks of direct contact co-culture of cells in the prior art are solved, and the effect of simplifying operation and improving efficiency is achieved.

CN223292566UActive Publication Date: 2025-09-02BEIJING LIFE SCIENCE ACADEMY CO LTD
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Patent Information

Application Number
CN202422408990.4
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-09-30
Publication Date
2025-09-02
Estimated Expiration
2034-09-30

AI Technical Summary

Technical Problem

Existing cell co-culture technologies are difficult to achieve direct contact co-culture of cells and are complex in operation, increasing the risk of contamination.

Method used

A double-sided adjustable cell co-culture device is designed. By setting a second culture container in the first culture container and regulating the up and down flip of the diaphragm with a rotating device, the cells are co-cultured on the upper and lower diaphragm, which simplifies operation and reduces the risk of contamination.

Benefits of technology

Direct contact co-culture of cells is achieved, operating steps are simplified, the risk of cell contamination is reduced, and the efficiency of cell co-culture is improved.

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Abstract

The utility model provides a double-sided adjustable cell co-culture device, and belongs to the technical field of cell culture. Comprising a first culture container and a second culture container, the second culture container is arranged in the first culture container, a gap is formed between the bottom of the second culture container and the bottom of the first culture container, and the bottom of the second culture container is communicated with the first culture container; a mounting frame is rotatably arranged at the bottom of the second culture container, and a diaphragm is embedded in the mounting frame; rotating devices are connected to the two sides of the mounting frame, and one end of each rotating device penetrates through the side walls of the first culture container and the second culture container and extends out of the first culture container; the rotating device can drive the mounting frame to rotate, and when the mounting frame rotates to a horizontal position, the mounting frame is in sealing contact with the bottom edge of the second culture container. According to the cell culture device, the cell culture efficiency is improved, the risk that the cells are polluted is reduced, and experimental conditions are provided for deeply exploring the mutual influence between the cells.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a double-sided adjustable cell co-culture device. Background Art

[0002] Cell co-culture refers to the co-cultivation of two or more cell types in the same culture system. Cell co-culture can simulate the real environment in the body and is of great significance for studying cell-cell interactions and their biological effects. Currently, cell co-culture technology is mainly divided into two categories: direct contact co-culture and indirect contact co-culture. In the direct contact co-culture mode, due to the direct interaction between cells, it is difficult to distinguish the biological effects of each cell after being stimulated by external stimuli. Compared with the direct contact co-culture mode, the indirect contact co-culture method generally uses transwell culture plates. However, the limitation of this method is that it only supports indirect contact co-culture, but not direct contact co-culture. Another co-culture method is to first inoculate one cell on one side of the culture plate, then remove the culture plate from the culture medium and flip it over, and inoculate another cell on the other side. However, the operation of this method is relatively complicated and time-consuming, and the risk of cell contamination is increased due to multiple handling and flipping operations. Utility Model Content

[0003] In response to the problems existing in the above-mentioned prior art, the present invention provides a double-sided adjustable cell co-culture device. The purpose of the present invention is to be able to co-culture cells on and below the diaphragm to perform direct contact cell culture, and to distinguish the respective biological effects of cells after being stimulated by external stimuli; and by operating the knob set on the outside of the culture container, the upper and lower sides of the cell culture surface inside the culture container can be adjusted, which can effectively reduce the risk of cell contamination and significantly improve the efficiency of cell culture.

[0004] To solve the above technical problems, the present invention provides a double-sided adjustable cell co-culture device, comprising a first culture container and a second culture container, wherein the second culture container is disposed within the first culture container, a gap is formed between the bottom of the second culture container and the bottom of the first culture container, and the bottom of the second culture container is connected to the first culture container;

[0005] A mounting frame is rotatably provided at the bottom of the second culture container, and a diaphragm is embedded in the mounting frame; a rotating device is connected to both sides of the mounting frame, and one end of the rotating device passes through the side walls of the first culture container and the second culture container and extends to the outside of the first culture container; the rotating device is capable of driving the mounting frame to rotate, and when the mounting frame rotates to a horizontal position, the mounting frame is in sealing contact with the bottom edge of the second culture container.

[0006] Furthermore, a plurality of hanging ears are provided on the upper end of the second culture container along the circumferential direction. The hanging ears are hung on the top end of the first culture container to fix the second culture container inside the first culture container.

[0007] Furthermore, the rotating device includes a connecting sleeve, a rotating column and a knob, and the side walls of the first culture container and the second culture container are provided with holes adapted to the connecting sleeve; one end of the connecting sleeve is detachably connected to the mounting frame, and the other end of the connecting sleeve passes through the holes of the second culture container and the first culture container and is fixedly connected to the rotating column, and the end of the rotating column away from the connecting sleeve is fixedly connected to the knob.

[0008] Furthermore, a portion of the connecting sleeve located outside the first culture container is provided with a fastener, and the fastener is threadedly connected to the connecting sleeve; a through groove is provided in the connecting sleeve, and the rotating column is clamped in the through groove.

[0009] Furthermore, the inner side wall of the through groove of the connecting sleeve is provided with two protrusions opposite to each other, each protrusion is provided with a groove, and the side wall of the rotating column is provided with two protrusions opposite to each other, and the protrusions are stuck in the grooves.

[0010] Furthermore, the diaphragm is made of elastic material.

[0011] Furthermore, the diaphragm has a porous structure.

[0012] Furthermore, the pore size of the porous structure in the membrane is smaller than the diameter of the cells to be cultured.

[0013] Furthermore, the pore size of the porous structure in the diaphragm is 0.3 microns.

[0014] The utility model provides a double-sided adjustable cell co-culture device, which achieves at least the following beneficial effects:

[0015] By setting a second culture container inside the first culture container, different cells are inoculated on the upper and lower culture surfaces at the bottom of the second culture container, respectively, which facilitates close observation and analysis of the two types of cells and distinguishes the respective biological effects of the cells after being stimulated by external stimuli; through a rotating device outside the first culture container, the up and down directions of the culture surface can be manually adjusted without removing the first culture container, which not only simplifies the operation steps and improves the convenience of use, but also effectively reduces the risk of cell contamination, thereby improving the overall efficiency of cell co-culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of a double-sided cell co-culture device according to one embodiment of the present invention;

[0017] Figure 2 This is a cross-sectional view of a connecting sleeve according to an embodiment of the present invention;

[0018] Figure 3 is a schematic diagram of a rotating column and a knob according to an embodiment of the present invention;

[0019] Figure 4 It is a schematic diagram of a mounting frame of an embodiment of the present utility model;

[0020] In the figure: 1, first culture container; 2, hanging ear; 3, second culture container; 4, diaphragm; 5, mounting frame; 6, knob; 7, rotating column; 8, connecting sleeve; 9, protrusion; 10, groove; 11, protrusion. DETAILED DESCRIPTION

[0021] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0023] In order to better understand the purpose, structure and function of the present invention, the double-sided adjustable cell co-culture device of the present invention is further described in detail below with reference to the accompanying drawings.

[0024] Example 1

[0025] like Figure 1 As shown, the present invention provides a double-sided adjustable cell co-culture device, comprising a first culture container 1 and a second culture container 3. The second culture container 3 is disposed within the first culture container 1, with a gap between the bottom of the second culture container 3 and the bottom of the first culture container 1. The bottom of the second culture container 3 is connected to the first culture container 1, so that the first culture container 1 and the second culture container 3 can share the same culture fluid.

[0026] A mounting frame 5 is rotatably provided at the bottom of the second culture container 3, and a diaphragm 4 is embedded in the mounting frame 5; a rotating device is connected to both sides of the mounting frame 5, and one end of the rotating device passes through the side walls of the first culture container 1 and the second culture container 3 and extends to the outside of the first culture container 1; the rotating device can drive the mounting frame 5 to rotate, and when the mounting frame 5 is rotated to a horizontal position, the mounting frame 5 is in sealing contact with the bottom edge of the second culture container 3.

[0027] like Figure 4 As shown, the installation frame 5 has a certain thickness for easy installation; the diaphragm 4 is embedded in the installation frame 5 and can be installed in the installation frame 5 by using a threaded connection.

[0028] Example 2

[0029] like Figure 1 As shown, the present invention provides a double-sided adjustable cell co-culture device, comprising a first culture container 1 and a second culture container 3. The second culture container 3 is disposed within the first culture container 1, with a gap between the bottom of the second culture container 3 and the bottom of the first culture container 1. The bottom of the second culture container 3 is connected to the first culture container 1, so that the first culture container 1 and the second culture container 3 can share the same culture fluid.

[0030] A mounting frame 5 is rotatably provided at the bottom of the second culture container 3, and a diaphragm 4 is embedded in the mounting frame 5; a rotating device is connected to both sides of the mounting frame 5, and one end of the rotating device passes through the side walls of the first culture container 1 and the second culture container 3 and extends to the outside of the first culture container 1; the rotating device can drive the mounting frame 5 to rotate, and when the mounting frame 5 is rotated to a horizontal position, the mounting frame 5 is in sealing contact with the bottom edge of the second culture container 3.

[0031] The difference between this embodiment and embodiment 1 is that:

[0032] like Figure 1 As shown, a plurality of hanging ears 2 are provided along the circumferential direction on the upper end of the second culture container 3. The hanging ears 2 are hung on the top of the first culture container 1 and are used to fix the second culture container 3 inside the first culture container 1. The hanging ears 2 in this embodiment are only exemplary and may be hooks or other components with a hanging function in other embodiments.

[0033] The rotating device includes a connecting sleeve 8, a rotating column 7 and a knob 6. The side walls of the first culture container 1 and the second culture container 3 are both provided with holes adapted to the connecting sleeve 8. One end of the connecting sleeve 8 is detachably connected to the mounting frame 5, for example, by a threaded connection that can be adjusted according to usage. The other end of the connecting sleeve 8 passes through the holes of the second culture container 3 and the first culture container 1 and is fixedly connected to the rotating column 7. The end of the rotating column 7 away from the connecting sleeve 8 is fixedly connected to the knob 6.

[0034] The connecting sleeve 8 is provided with a through groove, and the rotating column is clamped in the through groove; the portion of the connecting sleeve 8 located outside the first culture container 1 is provided with a fastener, and the fastener is threadedly connected to the connecting sleeve 8; in this embodiment, the fastener can be a nut. Specifically, the portion of the connecting sleeve 8 located outside the first culture container 1 is provided with an external thread. By sleeve-fitting a nut on the external threaded portion, the rotating device can be locked or unlocked relative to the first culture container 1 and the second culture container 3 by tightening or loosening the nut;

[0035] Furthermore, two protrusions 9 are relatively provided on the inner side walls of the through groove of the connecting sleeve 8, and each protrusion 9 is provided with a groove 10. The side walls of the rotating column 7 are relatively provided with two protrusions 11, and the protrusions 11 are stuck in the grooves 10. When rotating, the nut on the outside of the connecting sleeve 8 is loosened so that the connecting sleeve 8 is unlocked, and the rotating knob 6 drives the connecting sleeve 8 to rotate, thereby rotating the mounting frame 5 up and down, so that the diaphragm 4 can be flipped up and down. After the flipping is completed, the nut on the outside of the connecting sleeve 8 is tightened to lock the connecting sleeve 8 relative to the first culture container 1 and the second culture container 3.

[0036] A sealing strip may be provided on the outside of the mounting frame 5 to seal the mounting frame 5 to the bottom of the second culture container.

[0037] In this embodiment, the mounting frame 5, the knob 6 and the rotating column 7 are all made of polystyrene;

[0038] The diaphragm 4 is made of an elastic material, preferably, the diaphragm is made of polystyrene; the diaphragm 4 has a porous structure, the pore size of the porous structure in the diaphragm 4 is smaller than the diameter of the cells to be cultured in the first culture vessel and the second culture vessel, and can prevent the cells in the first culture container and the second culture container from passing through the diaphragm 4. Specifically, the diameter of the pores in the diaphragm is 0.3 microns.

[0039] The use process of the double-sided adjustable cell co-culture device of the utility model is as follows:

[0040] All containers and components have been sterilized, e.g. Figure 1As shown, cells are first inoculated at the bottom of the first culture container 1, and then the second culture container 3 is hung in the first culture container 1 through the hanging ear 2. The rotary button 6 on the first culture container 1 is connected to the mounting frame 5 on the side wall of the second culture container through the connecting sleeve 8, and the cells are inoculated on the first upward side of the diaphragm 4 at the bottom of the second culture container 3; after the cells adhere to the wall, the knob 6 is rotated to drive the connecting sleeve 8 to rotate, and then the diaphragm 4 is turned upside down, with the second side of the diaphragm 4 facing upward, and the cells are inoculated thereon; the device can realize the cultivation of multiple cells in the same culture environment, improve the efficiency of cell culture, and reduce the risk of contamination.

[0041] The parts of the present invention that are not described in detail are prior art, and their internal structures will not be described in detail here.

[0042] Although various embodiments of the present invention have been described in detail herein, it should be understood that the present invention is not limited to the specific embodiments described and illustrated herein. Other modifications and variations may be implemented by those skilled in the art without departing from the spirit and scope of the present invention. All such modifications and variations fall within the scope of the present invention. Furthermore, all components described herein may be replaced by other technically equivalent components.

Claims

1. A double-sided adjustable cell co-culture device, characterized in that: The invention comprises a first culture container and a second culture container, wherein the second culture container is arranged in the first culture container, a gap is formed between the bottom of the second culture container and the bottom of the first culture container, and the bottom of the second culture container is connected to the first culture container; A mounting frame is rotatably provided at the bottom of the second culture container, and a diaphragm is embedded in the mounting frame; a rotating device is connected to both sides of the mounting frame, and one end of the rotating device passes through the side walls of the first culture container and the second culture container and extends to the outside of the first culture container; the rotating device is capable of driving the mounting frame to rotate, and when the mounting frame rotates to a horizontal position, the mounting frame is in sealing contact with the bottom edge of the second culture container.

2. The double-sided adjustable cell co-culture device according to claim 1, characterized in that: A plurality of hanging ears are provided on the upper end of the second culture container along the circumferential direction, and the hanging ears are hung on the top end of the first culture container.

3. The double-sided adjustable cell co-culture device according to claim 1, characterized in that: The rotating device includes a connecting sleeve, a rotating column and a knob. The side walls of the first culture container and the second culture container are provided with holes adapted to the connecting sleeve; one end of the connecting sleeve is detachably connected to the mounting frame, and the other end of the connecting sleeve passes through the holes of the second culture container and the first culture container and is fixedly connected to the rotating column, and the end of the rotating column away from the connecting sleeve is fixedly connected to the knob.

4. The double-sided adjustable cell co-culture device according to claim 3, characterized in that: A fastener is provided on the portion of the connecting sleeve located outside the first culture container, and the fastener is threadedly connected to the connecting sleeve; a through groove is provided in the connecting sleeve, and the rotating column is clamped in the through groove.

5. The double-sided adjustable cell co-culture device according to claim 4, characterized in that: The inner side wall of the through groove of the connecting sleeve is provided with two protrusions opposite to each other, each protrusion is provided with a groove, and the side wall of the rotating column is provided with two protrusions opposite to each other, and the protrusions are clamped in the grooves.

6. The double-sided adjustable cell co-culture device according to any one of claims 1 to 5, characterized in that: The diaphragm is made of elastic material.

7. The double-sided adjustable cell co-culture device according to any one of claims 1 to 5, characterized in that: The separator has a porous structure.

8. The double-sided adjustable cell co-culture device according to claim 7, characterized in that: The pore size of the porous structure in the membrane is smaller than the diameter of the cells to be cultured.

9. The double-sided adjustable cell co-culture device according to claim 8, characterized in that: The pore size of the porous structure in the diaphragm is 0.3 microns.