Aquatic organism metabolite trapping device
By designing aquatic biological metabolites capture device and adopting modular design and automated processing, the problems of long and high cost of collecting aquatic biological metabolites are solved, and the efficiency of real-time capture and sample acquisition is achieved.
Patent Information
- Application Number
- CN202422474012.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-12
- Publication Date
- 2025-09-02
- Estimated Expiration
- 2034-10-12
AI Technical Summary
The existing culture devices and metabolite collection methods cannot meet the needs of real-time collection of urine, feces, etc. in aquatic organisms directly discharged into water, and the concentration of metabolites in aquatic organisms such as zebrafish and algae is low, and the existing methods are time-consuming and costly.
A water biological metabolite capture device is designed, including an ecological cylinder, oxygen delivery pump, water pump, capture column and four-way valve. Through a modular design, it realizes integration from culture to filtration to collection. It uses the filler of the pump and capture column to capture metabolites, and is automatically treated through organic solvent elution and cleaning water pipelines.
Real-time capture and automated processing of aquatic biological metabolites is realized, and a large number of experimental samples can be obtained accurately and in a timely manner, simplifying the operation process and reducing costs.
Smart Images
Figure CN223295936U_ABST
Abstract
Description
Technical Field
[0001] The utility model is used in the field of biological metabolism research, in particular to a device for capturing metabolites of aquatic organisms. Background Art
[0002] Metabolism is the science of the characteristics and patterns of chemical structural transformations of substances within living organisms. Structural changes in substances within the body occur through oxidation, reduction, decomposition, and combination, resulting in the formation of new metabolites. Metabolic research has a significant impact on ecology, pharmacokinetics, pharmacodynamics, toxicology, drug development, and other biomedical disciplines. The study of aquatic organisms is a crucial component of scientific research and industry, and they are important subjects for experiments related to environmental pollution, ecotoxicology, disease prevention, and drug discovery. By collecting and analyzing the contents of various parts of aquatic organisms, and examining blood, urine, feces, and bile to detect structural changes in metabolites, the metabolic transformation of substances can be studied.
[0003] Existing culture devices and metabolite collection methods cannot meet the necessary conditions for experimental research. The urine, feces, organic matter, etc. of aquatic organisms are directly discharged into the water and cannot be collected in real time. In addition, the concentration of algae metabolites of some aquatic organisms, such as zebrafish, is low. The existing methods cannot meet the experimental needs and have the difficulty of manual collection, which is time-consuming and costly. Utility Model Content
[0004] The purpose of the utility model is to solve at least one of the technical problems existing in the prior art and to provide a device for capturing metabolites of aquatic organisms.
[0005] The technical solution adopted by the utility model to solve its technical problems is:
[0006] A device for capturing metabolites of aquatic organisms comprises an aquatic organism life support system and a metabolite collection system. The aquatic organism life support system comprises an ecological tank and an oxygen supply pump. The metabolite collection system comprises a water pump and a capture column. The water inlet end of the water pump is connected to a water inlet pipeline, an organic solvent pipeline and a cleaning water pipeline. The water outlet end of the water pump is connected to the capture column. The capture column comprises a column body and a filler arranged inside the column body. One end of the column body forms a liquid inlet, and the other end of the column body forms a liquid outlet.
[0007] In combination with the above implementations, in some implementations, a four-way valve is provided at the water inlet end of the water pump, and the water inlet pipeline, the organic solvent pipeline and the cleaning water pipeline are connected to the four-way valve.
[0008] In combination with the above implementations, in some implementations, the water inlet pipe is connected to the ecological tank, and the water inlet pipe is provided with a filtering structure.
[0009] In combination with the above implementations, in some implementations, the filtration structure includes a filtration membrane.
[0010] In combination with the above implementations, in certain implementations, the metabolite collection system includes an organic solvent bottle, and the organic solvent pipeline is connected to the organic solvent bottle.
[0011] In combination with the above implementations, in certain implementations, the metabolite collection system includes a cleaning water bottle, and the cleaning water pipeline is connected to the cleaning water bottle.
[0012] In combination with the above implementations, in certain implementations, the metabolite collection system includes a collection bottle and a waste liquid bottle.
[0013] In combination with the above implementations, in some implementations, the aquatic life support system further includes a fluorescent lamp and a feeder, and the fluorescent lamp is arranged on the top of the ecological tank.
[0014] In combination with the above implementations, in some implementations, a controller is further included, and the fluorescent lamp, feeder, four-way valve, oxygen pump, and water pump are all connected to the controller.
[0015] In combination with the above implementations, in certain implementations, the eco-aquarium has a plurality of culture chambers separated from each other, the top of the eco-aquarium has a mounting frame, and the metabolite collection system and the controller are arranged on the mounting frame.
[0016] One of the above technical solutions has at least one of the following advantages or beneficial effects: In the technical solution of the present invention, the eco-tank is a place for cultivating experimental subjects. With the help of the aquatic life support system, experimental subjects including but not limited to fish and algae can be cultivated. The metabolites of the cultivated subjects are further collected by the metabolite collection system, that is, the water containing metabolites in the eco-tank is passed through a capture column by a water pump, and the metabolites are captured with the help of the filler of the capture column. The captured metabolites can be further eluted with the help of an organic solvent, and subsequent analysis and processing can be carried out. The technical solution of the present invention adopts a modular and integrated design, which can complete the integration from cultivation to filtration to collection, and can capture aquatic metabolites in real time, so as to obtain a large number of experimental samples accurately and timely.
[0017] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments in conjunction with the following drawings, in which:
[0019] Figure 1 This is a schematic structural diagram of an embodiment of the utility model from a first perspective;
[0020] Figure 2 This is a schematic structural diagram of an embodiment of the utility model from a second viewing angle;
[0021] Figure 3 This is a schematic structural diagram of an embodiment of the utility model from a third perspective;. DETAILED DESCRIPTION
[0022] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.
[0023] In the present invention, if there is a description of directions (up, down, left, right, front and back), it is only for the convenience of describing the technical solution of the present invention, and does not indicate or imply that the technical features referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.
[0024] In this utility model, "several" means one or more, "multiple" means more than two, "greater than," "less than," "exceeds," etc. are understood to exclude the number itself; "above," "below," "within," etc. are understood to include the number itself. In the description of this utility model, the use of "first" or "second" is solely for the purpose of distinguishing technical features and is not to be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.
[0025] In this utility model, unless otherwise expressly defined, terms such as "disposed," "installed," and "connected" should be interpreted broadly. For example, they may refer to direct connection or indirect connection through an intermediate medium; fixed connection or detachable connection or integral molding; mechanical connection or electrical connection or mutual communication; and internal connection between two components or interaction between two components. Those skilled in the art can reasonably determine the specific meanings of these terms in this utility model based on the specific content of the technical solution.
[0026] See also Figure 1 、 Figure 2 、 Figure 3The embodiment of the present utility model provides an aquatic organism metabolite capture device, including an aquatic organism life support system and a metabolite collection system. The aquatic organism life support system includes an eco-tank 1 and an oxygen pump 2. The eco-tank 1 is a place for cultivating experimental objects. The shape of the eco-tank 1 can be round, square, irregular, etc., and the material can be glass, plastic, etc. The metabolite collection system includes a water pump 3 and a capture column 4. The water inlet end of the water pump 3 is connected to the water inlet pipeline 5, the organic solvent pipeline 6 and the cleaning water pipeline 7. The water outlet end of the water pump 3 is connected to the capture column 4. The capture column 4 includes a column body and a filler arranged inside the column body. One end of the column body forms a liquid inlet, and the other end of the column body forms a liquid outlet. The capture column 4 is used to capture the metabolites of the experimental object. The metabolites include small molecule metabolites such as amino acids, peptides, proteins, etc. commonly found in aquatic organisms, as well as exogenous drugs and environmental pollutants and their metabolic conversion products. During the capture process, the water pump 3 draws the experimental subject's water into the liquid inlet and out the liquid outlet, during which the metabolites of the experimental subject are captured by the filler. The capture column 4 can be configured as a replaceable component, allowing different capture columns 4 to be replaced according to the different metabolites to be collected.
[0027] In the technical solution of the present invention, eco-aquarium 1 serves as a place for cultivating experimental subjects. With the aid of an aquatic life support system, experimental subjects, including but not limited to fish, algae, and plants, can be cultivated. Metabolites of the cultivated subjects are further collected by a metabolite collection system. Specifically, a water pump 3 draws water containing metabolites from eco-aquarium 1 through a capture column 4. The metabolites are captured by the packing of capture column 4. The captured metabolites can then be eluted with an organic solvent for subsequent analysis and processing. The technical solution of the present invention adopts a modular, integrated design, capable of integrating cultivation, filtration, and collection, enabling real-time capture of aquatic metabolites, and accurately and timely obtaining a large number of experimental samples.
[0028] In some embodiments, the capture column 4 is a chromatographic column, such as a C8 chromatographic column, a C18 chromatographic column, a CAX chromatographic column, or an HLB chromatographic column, etc., and its outlet end can be connected to a detection instrument such as a high performance liquid chromatography.
[0029] In some embodiments, see Figure 1 、 Figure 2 、 Figure 3The water inlet of the water pump 3 is provided with a four-way valve 8, to which the water inlet line 5, the organic solvent line 6, and the rinse water line 7 are connected. The four-way valve 8 enables the flow lines to switch between different functions. For example, when capturing metabolites, the four-way valve 8 controls the water inlet line 5 to connect to the capture column 4, where it flows through the capture column 4 and then back to the eco-aquarium 1. When eluting metabolites, the four-way valve 8 controls the organic solvent line 6 to connect to the capture column 4. And when cleaning the capture column 4, the four-way valve 8 controls the rinse water line 7 to connect to the capture column 4. The provision of the four-way valve 8 in this embodiment allows for rapid function switching and simplifies the structure.
[0030] In some embodiments, see Figure 1 、 Figure 2 、 Figure 3 The water inlet pipe 5 is connected to the eco-tank 1 and is equipped with a filter structure 9. The filter structure 9 includes a detachable filter membrane, which can filter large particles in the water and collect large metabolites. This filter not only filters large impurities to prevent pipe blockage, but also can be used to collect larger solid feces.
[0031] In some embodiments, see Figure 1 、 Figure 2 、 Figure 3 The metabolite collection system includes an organic solvent bottle 10, to which an organic solvent line 6 is connected. An organic solvent such as methanol, acetonitrile, acetone, or isopropanol can be placed. The organic solvent is used to elute the metabolites from the capture column 4. Specifically, the organic solvent is connected to the capture column 4 through the organic solvent line 6 and flows to the capture column 4.
[0032] Further, see Figure 1 、 Figure 2 、 Figure 3 The metabolite collection system includes a collection bottle 11. The metabolites eluted by the organic solvent are led out from the capture column 4 and then flow into the collection bottle 11 for collection.
[0033] In some embodiments, see Figure 1 、 Figure 2 、 Figure 3 The metabolite collection system includes a cleaning water bottle 12, and the cleaning water pipeline 7 is connected to the cleaning water bottle 12. The cleaning water can be provided with pure water, ammonium formate brine, sodium chloride brine, etc., which is used to clean the capture column 4 and the pipeline. That is, the cleaning water is connected through the cleaning water pipeline 7 and flows to the capture column 4.
[0034] Further, see Figure 1 、 Figure 2 、 Figure 3The metabolite collection system includes a waste liquid bottle 13. The wastewater after washing with the cleaning water is led out from the capture column 4 and flows into the waste liquid bottle 13 for collection to avoid contaminating the aquatic life support system of the ecological tank 1.
[0035] In some embodiments, see Figure 1 、 Figure 2 、 Figure 3 The aquatic life support system also includes a fluorescent lamp 14 and a feeder 15. The fluorescent lamp 14 is arranged on the top of the ecological tank 1. The fluorescent lamp 14 can provide light for the aquatic organisms in the ecological tank 1, and the feeder 15 is used to provide food for the aquatic organisms in the ecological tank 1.
[0036] Further, see Figure 1 、 Figure 2 、 Figure 3 The aquatic organism metabolite capture device also includes a controller 16, and the fluorescent lamp 14, feeder 15, four-way valve 8, oxygen pump 2, and water pump 3 are all connected to the controller 16. The controller 16 controls the timing switch of the water pump, the flow path switching of the four-way valve 8, the switch of the oxygen pump 2, the timing feeding switch, the lighting time of the fluorescent lamp 14, etc. With the help of the controller 16, the metabolites of aquatic organisms in the water can be filtered and enriched in real time, and a large number of experimental samples can be obtained accurately and timely. Metabolites of various aquatic organisms can be collected, the sampling time can be controlled, the collected samples can be automatically eluted, and the pipelines can be automatically cleaned without affecting the normal growth environment of the experimental objects. This embodiment has an automatic switch control function, which facilitates experimental operation and makes the experiment more accurate and simpler.
[0037] In some embodiments, the ecological tank 1 has one or more culture chambers separated from each other. When multiple culture chambers separated from each other are provided, multiple experimental objects can be cultured simultaneously, thereby improving the efficiency of the experiment.
[0038] See also Figure 1 、 Figure 2 、 Figure 3 The top of the ecological tank 1 has a mounting frame 17, and the mounting frame 17 can be provided with a multi-layer storage plate. The metabolite collection system and the controller 16 are arranged on the mounting frame 17, and the entire structure is more compact.
[0039] The specific usage is as follows:
[0040] 1. Feed aquatic organisms such as zebrafish and transfer them to the ecological tank 1.
[0041] Switch the system flow path to the ecological tank 1, the water inlet pipe 5 to the capture column 4, start the water pump 3, set the pumping time to 24 hours, and then place the water outlet of the capture column 4 into the ecological tank 1.
[0042] After pumping out water for 24 hours, remove the capture column 4 from the ecological tank 1 and place it in the collection bottle 11 or connect it to the chromatograph. Switch the flow path from the organic solvent pipeline 6 to the capture column 4, and set the time to 20 minutes.
[0043] After the elution is completed, switch to the cleaning water pipeline 7 and the capture column 4 for cleaning for 20 minutes.
[0044] The filter membrane was removed and the solid material was collected.
[0045] 2. Transfer aquatic organisms such as seaweed to the ecological tank 1.
[0046] After turning on the fluorescent light for 24 hours;
[0047] Switch the system flow path to the ecological tank 1, the water inlet pipe 5 to the capture column 4, start the water pump 3, set the pumping time to 24 hours, and then place the water outlet of the capture column 4 into the ecological tank 1.
[0048] After pumping out water for 24 hours, remove the capture column 4 from the ecological tank 1 and place it in the collection bottle 11 or connect it to the chromatograph. Switch the flow path from the organic solvent pipeline 6 to the capture column 4, and set the time to 20 minutes.
[0049] After the elution is completed, switch to the cleaning water pipeline 7 and the capture column 4 for cleaning for 20 minutes.
[0050] Remove the filter membrane and collect the enriched metabolites.
[0051] 3. Transfer aquatic plants such as lotus to ecological tank 1.
[0052] After turning on the fluorescent lamp and supplying oxygen for 24 hours;
[0053] Switch the system flow path to the ecological tank 1, the water inlet pipe 5 to the capture column 4, start the water pump 3, set the pumping time to 24 hours, and then place the water outlet of the capture column 4 into the ecological tank 1.
[0054] After pumping out water for 24 hours, remove the capture column 4 from the ecological tank 1 and place it in the collection bottle 11 or connect it to the chromatograph. Switch the flow path from the organic solvent pipeline 6 to the capture column 4, and set the time to 20 minutes.
[0055] After the elution is completed, switch to the cleaning water pipeline 7 and the capture column 4 for cleaning for 20 minutes.
[0056] Throughout this specification, references to terms such as "example," "embodiment," or "some embodiments" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
[0057] Of course, the invention is not limited to the above-mentioned embodiments. Those skilled in the art may make equivalent modifications or substitutions without violating the spirit of the invention. These equivalent modifications or substitutions are all within the scope defined by the claims of this application.
Claims
1. A device for capturing metabolites of aquatic organisms, characterized in that: It includes an aquatic life support system and a metabolite collection system. The aquatic life support system includes an ecological tank and an oxygen supply pump. The metabolite collection system includes a water pump and a capture column. The water inlet end of the water pump is connected to the water inlet pipeline, the organic solvent pipeline and the cleaning water pipeline. The water outlet end of the water pump is connected to the capture column. The capture column includes a column body and a filler arranged inside the column body. One end of the column body forms a liquid inlet, and the other end of the column body forms a liquid outlet.
2. The aquatic organism metabolite capture device according to claim 1, characterized in that: A four-way valve is provided at the water inlet end of the water pump, and the water inlet pipeline, the organic solvent pipeline and the cleaning water pipeline are connected to the four-way valve.
3. The aquatic organism metabolite capture device according to claim 1, characterized in that: The water inlet pipeline is connected to the ecological tank, and the water inlet pipeline is provided with a filtering structure.
4. The aquatic organism metabolite capture device according to claim 3, characterized in that: The filtration structure includes a filtration membrane.
5. The aquatic organism metabolite capture device according to claim 1, characterized in that: The metabolite collection system comprises an organic solvent bottle, and the organic solvent pipeline is connected to the organic solvent bottle.
6. The aquatic organism metabolite capture device according to claim 1, characterized in that: The metabolite collection system includes a cleaning water bottle, and the cleaning water pipeline is connected to the cleaning water bottle.
7. The aquatic organism metabolite capture device according to claim 1, characterized in that: The metabolite collection system includes a collection bottle and a waste liquid bottle.
8. The aquatic organism metabolite capture device according to claim 2, characterized in that: The aquatic life support system further comprises a fluorescent lamp and a feeder, wherein the fluorescent lamp is arranged on the top of the ecological tank.
9. The aquatic organism metabolite capture device according to claim 8, characterized in that: The device also includes a controller, and the fluorescent lamp, feeder, four-way valve, oxygen supply pump and water pump are all connected to the controller.
10. The aquatic organism metabolite capture device according to claim 9, characterized in that: The ecological tank has a plurality of culture chambers separated from each other. The top of the ecological tank is provided with a mounting frame, and the metabolite collection system and the controller are arranged on the mounting frame.