Cell culture device for cell drug screening

Abstract

The utility model relates to the technical field of cell culture devices, in particular to a cell culture device for screening cell drugs, which comprises a base, a tank body, a vibration motor, a vibration component, an arc-shaped positioning block, a bidirectional adjusting screw rod, an arc-shaped clamping plate, an adjusting component, a blanking hopper, a rotating shaft, a scraper blade and a stirring component, a tank body is arranged above a base, a vibration motor is arranged at the top of the tank body, a vibration assembly is arranged on one side of the tank body, an arc-shaped positioning block is arranged on the outer side of the tank body, a two-way adjusting lead screw is arranged on one side of the tank body, an arc-shaped clamping plate is arranged on the outer side of the two-way adjusting lead screw, and an adjusting assembly is arranged on one side of the arc-shaped clamping plate. A discharging hopper is arranged on the bottom face of the tank, a rotating shaft is arranged on the inner side of the tank, a scraper is arranged on the outer side of the bottom end of the rotating shaft, and a stirring assembly is arranged on the outer side of the rotating shaft. By means of continuous vibration and wall-attached stirring, the cell movement speed is increased, cells are fully mixed, the cells are prevented from sinking to the bottom, and therefore the practical value of the device is enhanced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, in particular to a cell culture device for cell drug screening. Background Art

[0002] In the production of modern medical drugs, some drugs need to be produced through cells. During the production, the cells need to be cultured in advance using a cell culture device. When culturing the cells, a stirring structure is needed to accelerate the cell movement in the culture device.

[0003] Most of the current cell culture devices for cell drug screening have relatively simple stirring structures, which have poor cell mixing effects. Cells tend to sink to the bottom of the device, resulting in insufficient cell mixing reaction and affecting the cell culture effect.

[0004] Therefore, in view of the fact that the existing cell culture device for cell drug screening does not stir sufficiently and the cells easily sink to the bottom, a cell culture device for cell drug screening can be designed. By means of continuous vibration and wall stirring, the cell movement rate can be accelerated, the cells can be ensured to be fully mixed, and the cells can be prevented from sinking to the bottom, thereby enhancing the practical value of the device. Utility Model Content

[0005] In order to overcome the problem that most cell culture devices for cell drug screening have simple stirring structures, the effect of mixing cells is poor, and cells easily sink to the bottom of the device, resulting in insufficient cell mixing reaction.

[0006] The technical solution of the utility model is: a cell culture device for cell drug screening, comprising a base, a tank body, a vibration motor, a vibration component, an arc-shaped positioning block, a two-way adjustment screw rod, an arc-shaped clamping plate, an adjustment component, a discharge hopper, a rotating shaft, a scraper, a stirring component and a discharge component; a tank body is arranged above the base, a vibration motor is arranged on the top of the tank body, the output end of the vibration motor is connected to the tank body, a vibration component is arranged on one side of the tank body, an arc-shaped positioning block is arranged on the outside of the tank body, a two-way adjustment screw rod is arranged on one side of the tank body, an arc-shaped clamping plate is arranged on the outside of the two-way adjustment screw rod, an adjustment component is arranged on one side of the arc-shaped clamping plate, a discharge hopper is arranged on the bottom surface of the tank body, a rotating shaft is arranged on the inside of the tank body, the rotating shaft and the tank body are rotatably connected to each other, a scraper is arranged on the outside of the bottom end of the rotating shaft, a stirring component is arranged on the outside of the rotating shaft, and discharge components are arranged at both ends of the tank body.

[0007] Preferably, the cell stock solution is injected into the tank body through the discharge component, and the vibration motor is used to drive the tank body to vibrate. The vibration component is used to ensure that the tank body vibrates stably on the base. At the same time, the stirring component is used to drive the rotating shaft to rotate, and the rotating shaft drives the scraper to rotate. The scraper is used to scrape off the cell stock solution deposited in the discharge hopper, thereby continuously stirring the cell stock solution in the tank body. When discharging, the vibration motor is stopped, and the bidirectional adjustment screw is driven to rotate through the adjustment component. The bidirectional adjustment screw is rotated to adjust the spacing between the two sets of arc-shaped clamping plates, so that the arc-shaped clamping plates clamp the arc-shaped positioning blocks and fix the tank body, so that the tank body is stationary. The raw materials in the tank body are discharged by the discharge component, thereby accelerating the cell movement rate, ensuring that the cells are fully mixed, avoiding the effect of cell sinking to the bottom, and enhancing the practical value of the device.

[0008] Preferably, the vibration assembly includes a bracket, a support frame and a connecting spring; the bracket is provided on the bottom surface of the tank body, the bracket is provided on the upper surface of the base, the upper surface of the base is provided with a support frame, the bottom surface of the top of the support frame is provided with a connecting spring, there are multiple groups of connecting springs, and the other end of the connecting spring is connected to the top of the tank body.

[0009] Preferably, the adjustment assembly includes a fixed plate, a drive motor and a connecting block; the upper surface of the base is provided with a fixed plate, the top of the fixed plate is provided with a drive motor, and the outer side of the bidirectional adjustment screw is provided with a connecting block.

[0010] Preferably, the bidirectional adjustment screw is arranged on the inner side of the fixed plate, the output end of the drive motor is connected to the bidirectional adjustment screw, two groups of connecting blocks are provided, and an arc-shaped clamping plate is provided on one side of the connecting block, and the arc-shaped clamping plate and the limiting groove of the arc-shaped positioning block are interlocked.

[0011] Preferably, the stirring assembly includes a rotating motor, a stirring rod and a connecting rod; the rotating motor is provided at the top of the tank body, the stirring rod is provided on the outside of the rotating shaft, and the connecting rod is provided on the outside of the bottom end of the rotating shaft.

[0012] Preferably, the output end of the rotating motor is connected to the rotating shaft, multiple groups of stirring rods are provided, multiple groups of connecting rods are provided, and a scraper is provided at one end of the connecting rod, and the scraper is closely attached to the inner wall of the lower hopper.

[0013] Preferably, the unloading component includes a feed pipe, a discharge pipe and a solenoid valve; a feed pipe is provided at the top of the tank body, and the feed pipe and the tank body are interconnected; a discharge pipe is provided at the bottom end of the unloading hopper, and the discharge pipe is also interconnected with the tank body, and a solenoid valve is provided on the outside of the discharge pipe.

[0014] Beneficial effects of the utility model:

[0015] 1. When culturing cells, inject the cell stock solution into the tank body on the base, use the vibration motor to drive the tank body to vibrate, and at the same time, rotate the shaft to drive the scraper to rotate, and use the scraper to scrape the cell stock solution deposited in the lower hopper, thereby continuously stirring the cell stock solution in the tank body. When discharging, stop the vibration motor, rotate the two-way adjustment screw to adjust the distance between the two sets of arc-shaped clamping plates, so that the arc-shaped clamping plates clamp the arc-shaped positioning blocks, make the tank body still, and discharge the raw materials in the tank body, so as to solve the problem of insufficient stirring and easy sinking of cells in most cell culture devices for cell drug screening, and effectively enhance the practical value of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Shown is a schematic diagram of the three-dimensional structure of a cell culture device for cell drug screening according to the present invention;

[0017] Figure 2 Shown is a schematic diagram of the three-dimensional structure of a vibration component of a cell culture device for cell drug screening according to the present invention;

[0018] Figure 3 Shown is a schematic diagram of the three-dimensional structure of a bidirectional adjustment screw rod of a cell culture device for cell drug screening of the present invention;

[0019] Figure 4 Shown is a schematic diagram of the cross-sectional three-dimensional structure of a stirring component of a cell culture device for cell drug screening according to the present invention.

[0020] Explanation of the accompanying drawings: 1. Base; 2. Tank body; 3. Vibration motor; 301. Bracket; 302. Support frame; 303. Connecting spring; 4. Arc-shaped positioning block; 401. Bidirectional adjustment screw rod; 402. Arc-shaped clamping plate; 403. Fixed plate; 404. Driving motor; 405. Connecting block; 5. Lower hopper; 501. Rotating shaft; 502. Scraper; 503. Rotating motor; 504. Stirring rod; 505. Connecting rod; 601. Feed pipe; 602. Discharge pipe; 603. Solenoid valve. DETAILED DESCRIPTION

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] See also Figures 1-4The utility model provides an embodiment: a cell culture device for cell drug screening, comprising a base 1, a tank body 2, a vibration motor 3, a vibration assembly, an arc-shaped positioning block 4, a two-way adjustment screw rod 401, an arc-shaped clamping plate 402, an adjustment assembly, a discharge hopper 5, a rotating shaft 501, a scraper 502, a stirring assembly and a discharge assembly; a tank body 2 is arranged above the base 1, a vibration motor 3 is arranged on the top of the tank body 2, an output end of the vibration motor 3 is connected to the tank body 2, and a vibration assembly is arranged on one side of the tank body 2. An arc-shaped positioning block 4 is provided on the outside of the tank body 2, a two-way adjustment screw rod 401 is provided on one side of the tank body 2, an arc-shaped clamping plate 402 is provided on the outside of the two-way adjustment screw rod 401, an adjustment component is provided on one side of the arc-shaped clamping plate 402, a discharge hopper 5 is provided on the bottom surface of the tank body 2, a rotating shaft 501 is provided on the inside of the tank body 2, the rotating shaft 501 and the tank body 2 are rotatably connected to each other, a scraper 502 is provided on the outside of the bottom end of the rotating shaft 501, a stirring component is provided on the outside of the rotating shaft 501, and a discharge component is provided at both ends of the tank body 2.

[0023] See also Figure 2-Figure 4 In this embodiment, the vibration component includes a bracket 301, a support frame 302 and a connecting spring 303; the bottom surface of the tank body 2 is provided with a bracket 301, the bracket 301 is provided on the upper surface of the base 1, the upper surface of the base 1 is provided with a support frame 302, the bottom surface of the top of the support frame 302 is provided with a connecting spring 303, and there are multiple groups of connecting springs 303. The other end of the connecting spring 303 is connected to the top of the tank body 2. The tank body 2 is supported by the bracket 301, the connecting spring 303 is fixed by the support frame 302, and the connecting spring 303 is connected and fixed by the connecting spring 303, thereby effectively ensuring the stable vibration of the tank body 2; the adjustment component includes a fixed plate 403, a driving motor 404 and a connecting block 405; the upper surface of the base 1 is provided with a fixed plate 40 3. A driving motor 404 is provided at the top of the fixed plate 403. The output end of the driving motor 404 is connected to the bidirectional adjustment screw rod 401. The bidirectional adjustment screw rod 401 is provided on the inner side of the fixed plate 403. A connecting block 405 is provided on the outer side of the bidirectional adjustment screw rod 401. Two groups of connecting blocks 405 are provided. An arc-shaped clamping plate 402 is provided on one side of the connecting block 405. The arc-shaped clamping plate 402 and the limiting groove of the arc-shaped positioning block 4 are interlocked with each other. The position of the bidirectional adjustment screw rod 401 is fixed by the fixed plate 403. The driving motor 404 is used to drive the bidirectional adjustment screw rod 401 to rotate. The bidirectional adjustment screw rod 401 is rotated to flexibly adjust the distance between the two groups of connecting blocks 405, so that the arc-shaped clamping plate 402 on the connecting block 405 is flexibly clamped into the arc-shaped positioning block 4, thereby clamping and fixing the tank body 2.

[0024] The stirring assembly includes a rotating motor 503, a stirring rod 504 and a connecting rod 505; a rotating motor 503 is provided at the top of the tank body 2, and the output end of the rotating motor 503 is connected to the rotating shaft 501, and a stirring rod 504 is provided on the outside of the rotating shaft 501, and the stirring rod 504 is provided in multiple groups. A connecting rod 505 is provided on the outside of the bottom end of the rotating shaft 501, and the connecting rod 505 is provided in multiple groups. A scraper 502 is provided at one end of the connecting rod 505, and the scraper 502 is closely attached to the inner wall of the lower hopper 5, and the rotating shaft 501 is driven to rotate by the rotating motor 503, and the rotating shaft 501 drives the stirring rod 504 to rotate, and at the same time drives the scraper on the connecting rod 505 The plate 502 rotates close to the inner wall of the lower hopper 5, thereby fully stirring the cell stock solution inside the tank body 2; the lowering component includes a feed pipe 601, a discharge pipe 602 and a solenoid valve 603; a feed pipe 601 is provided at the top of the tank body 2, and the feed pipe 601 is interconnected with the tank body 2, and a discharge pipe 602 is provided at the bottom end of the lower hopper 5, and the discharge pipe 602 is also interconnected with the tank body 2, and a solenoid valve 603 is provided on the outside of the discharge pipe 602, and the cell stock solution is injected into the tank body 2 through the feed pipe 601, and the solenoid valve 603 is used to control the opening and closing of the discharge pipe 602, and the cell stock solution in the tank body 2 is discharged through the discharge pipe 602.

[0025] Before culturing cells, the tank body 2 is supported by the bracket 301 and fixed on the base 1. The cell stock solution is injected into the feed pipe 601. Then, the feed pipe 601 is closed and the cell stock solution is injected into the tank body 2 from the feed pipe 601.

[0026] When culturing cells, the vibrating motor 3 drives the tank body 2 to vibrate continuously, causing the connecting spring 303 on the support frame 302 to continuously stretch and vibrate, thereby accelerating the movement of the cell stock solution in the tank body 2;

[0027] At the same time, the stirring rod 504 on the rotating shaft 501 is rotated by the rotating motor 503 to stir the cell stock solution in the tank 2. The rotating shaft 501 can also drive the connecting rod 505 to rotate, and the rotating connecting rod 505 drives the scraper 502 to scrape the cell stock solution deposited in the lower hopper 5;

[0028] When discharging the cell stock solution, turn off the vibration motor 3, and drive the bidirectional adjustment screw 401 in the fixed plate 403 to rotate through the driving motor 404. Rotate the bidirectional adjustment screw 401 to adjust the distance between the two sets of connecting blocks 405, and clamp the arc-shaped clamping plate 402 into the arc-shaped positioning block 4 to make the tank body 2 stationary. Open the discharge pipe 602 through the solenoid valve 603, and use the lower hopper 5 to discharge the cell stock solution in the tank body 2.

[0029] Through the above steps, the cell stock solution is injected into the tank body 2 through the blanking component, the vibration motor 3 is used to drive the tank body 2 to vibrate, and the vibration component is used to ensure that the tank body 2 vibrates stably on the base 1. At the same time, the stirring component is used to drive the rotating shaft 501 to rotate, and the rotating shaft 501 drives the scraper 502 to rotate, and the scraper 502 is used to scrape the cell stock solution deposited in the blanking hopper 5, so as to continuously stir the cell stock solution in the tank body 2. When discharging, the vibration motor 3 is stopped, and the bidirectional adjustment screw 401 is driven to rotate through the adjustment component, and the bidirectional adjustment screw 401 is rotated to adjust the spacing between the two sets of arc clamping plates 402, so that the arc clamping plate 402 clamps the arc positioning block 4, so that the tank body 2 is stationary, and the raw materials in the tank body 2 are discharged by the blanking component, thereby accelerating the cell movement rate, ensuring that the cells are fully mixed, and preventing the cells from sinking to the bottom.

[0030] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the purpose of the present invention.

Claims

1. A cell culture device for cell drug screening, comprising a base (1) and a tank (2); characterized in that: The invention also includes a vibration motor (3), a vibration component, an arc-shaped positioning block (4), a two-way adjustment screw rod (401), an arc-shaped clamping plate (402), an adjustment component, a discharge hopper (5), a rotating shaft (501), a scraper (502), a stirring component and a discharge component; a tank body (2) is arranged above the base (1), a vibration motor (3) is arranged on the top of the tank body (2), an output end of the vibration motor (3) is connected to the tank body (2), a vibration component is arranged on one side of the tank body (2), an arc-shaped positioning block (4) is arranged on the outside of the tank body (2), and the tank body (2) is provided with a plurality of vibration components. A bidirectional adjustment screw rod (401) is provided on one side of the tank body (2), an arc-shaped clamping plate (402) is provided on the outer side of the bidirectional adjustment screw rod (401), an adjustment component is provided on one side of the arc-shaped clamping plate (402), a discharge hopper (5) is provided on the bottom surface of the tank body (2), a rotating shaft (501) is provided on the inner side of the tank body (2), the rotating shaft (501) and the tank body (2) are rotatably connected to each other, a scraper (502) is provided on the outer side of the bottom end of the rotating shaft (501), a stirring component is provided on the outer side of the rotating shaft (501), and a discharge component is provided at both ends of the tank body (2).

2. The cell culture device for cell drug screening according to claim 1, characterized in that: The vibration component comprises a bracket (301), a support frame (302) and a connecting spring (303); the bottom surface of the tank body (2) is provided with the bracket (301), the bracket (301) is provided on the upper surface of the base (1), the upper surface of the base (1) is provided with the support frame (302), the bottom surface of the top of the support frame (302) is provided with a connecting spring (303), multiple groups of connecting springs (303) are provided, and the other end of the connecting spring (303) is connected to the top end of the tank body (2).

3. The cell culture device for cell drug screening according to claim 1, characterized in that: The adjustment component comprises a fixed plate (403), a driving motor (404) and a connecting block (405); the upper surface of the base (1) is provided with the fixed plate (403), the top end of the fixed plate (403) is provided with the driving motor (404), and the outer side of the bidirectional adjustment screw rod (401) is provided with the connecting block (405).

4. The cell culture device for cell drug screening according to claim 3, characterized in that: A bidirectional adjustment screw (401) is arranged on the inner side of the fixed plate (403), the output end of the drive motor (404) is connected to the bidirectional adjustment screw (401), two groups of connecting blocks (405) are provided, and an arc-shaped clamping plate (402) is provided on one side of the connecting block (405), and the arc-shaped clamping plate (402) is interlocked with the limiting groove of the arc-shaped positioning block (4).

5. The cell culture device for cell drug screening according to claim 3, characterized in that: The stirring assembly comprises a rotating motor (503), a stirring rod (504) and a connecting rod (505); the rotating motor (503) is arranged at the top of the tank body (2), the stirring rod (504) is arranged on the outside of the rotating shaft (501), and the connecting rod (505) is arranged on the outside of the bottom end of the rotating shaft (501).

6. The cell culture device for cell drug screening according to claim 5, characterized in that: The output end of the rotating motor (503) is connected to the rotating shaft (501), multiple groups of stirring rods (504) are provided, multiple groups of connecting rods (505) are provided, and one end of the connecting rod (505) is provided with a scraper (502), and the scraper (502) is closely attached to the inner wall of the lower hopper (5).

7. The cell culture device for cell drug screening according to claim 6, characterized in that: The material discharge assembly comprises a feed pipe (601), a discharge pipe (602) and a solenoid valve (603); the top of the tank body (2) is provided with a feed pipe (601), the feed pipe (601) and the tank body (2) are mutually connected; the bottom of the discharge hopper (5) is provided with a discharge pipe (602), the discharge pipe (602) is also mutually connected with the tank body (2), and the outer side of the discharge pipe (602) is provided with a solenoid valve (603).

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