Simple and convenient entomopathogenic nematode culture and collection device

Abstract

The utility model provides a simple and convenient entomopathogenic nematode culture and collection device which comprises a culture container, supporting legs are fixedly installed on the lower surface of the culture container, a carrying block is placed in the culture container, the upper surface of the carrying block is covered with a layer of water absorption filter paper, and the size of the water absorption filter paper is slightly larger than that of the carrying block. And the excessive part of the water absorption filter paper is in contact with the lower bottom surface of the culture container. The culture and collection of the entomopathogenic nematodes are integrated, a large amount of entomopathogenic nematodes can be obtained in a short time, and scientific research and application requirements are met; the method is very simple and convenient in operation procedure, is suitable for culture of entomopathogenic nematodes, and remarkably reduces operation time and labor cost. The device also has the advantages of simplicity in operation, high efficiency, strong accuracy and the like, is a reasonable device for separating and collecting the entomopathogenic nematodes, and has important application value and scientific significance in culture and collection of the entomopathogenic nematodes.

Description

Technical Field

[0001] The utility model relates to the field of agricultural scientific research, and in particular to a simple and convenient culturing and collecting device for entomopathogenic nematodes. Background Art

[0002] Entomopathogenic nematodes are valued as a biological control method due to their wide insecticide range, ability to actively seek out their hosts, and their lack of environmental or plant side effects. They form a symbiotic relationship with specific bacteria, invading the host insects, releasing the bacteria and multiplying, producing toxins that kill the insects. This biological control method is not only rapid but also environmentally friendly, making it a sustainable agricultural pest management strategy.

[0003] The currently commonly used White Trap method has limited pipette capacity, which greatly limits the collection speed and increases time consumption. In addition, the collector needs to be frequently rehydrated due to the natural evaporation of water, which takes up daily time. The corpses of entomopathogenic nematode hosts may attract other insects to lay eggs on their corpses, resulting in contamination of the nematode suspension, which in turn affects the subsequent culture operation process.

[0004] In view of this, it is necessary to design a device that can collect entomopathogenic nematode suspension efficiently, conveniently and hygienically. Utility Model Content

[0005] In view of the deficiencies in the prior art, the present invention provides a simple and convenient device for cultivating and collecting entomopathogenic nematodes, which solves the problems mentioned in the background art.

[0006] In order to achieve the above-mentioned purpose of the utility model, the utility model provides the following technical solutions:

[0007] A simple entomopathogenic nematode culture and collection device is provided to solve the above problems.

[0008] The specific application is as follows:

[0009] It includes a culture container, the lower surface of which is fixedly equipped with support legs, and a loading block is placed inside the culture container, the upper surface of the loading block is covered with a layer of absorbent filter paper, and the size of the absorbent filter paper is slightly larger than the loading block, and the excess part of the absorbent filter paper is in contact with the lower bottom surface of the culture container, the top of the culture container is provided with an insect-proof net, and the bottom of the culture container is fixedly equipped with a liquid outlet pipe, and a collection container is provided on the lower side of the liquid outlet pipe.

[0010] As a preferred technical solution of the present application, the bottom surface of the culture container is provided with a slope inclined toward the liquid outlet pipe, and the object loading block is arranged in a rectangular parallelepiped.

[0011] As a preferred technical solution of the present application, a fixing frame is fixedly installed inside the top of the culture container, and an installation frame is provided on the upper side of the fixing frame, and the insect-proof net is fixedly installed inside the installation frame.

[0012] As a preferred technical solution of this application, a water tank is provided on the upper side of the culture container, and a support frame is fixedly installed on the bottom of the water tank. A water pipe is fixedly installed on the lower surface of the water tank, and the other end of the water pipe extends into the interior of the culture container.

[0013] As a preferred technical solution of the present application, an automatic water valve is fixedly installed at the other end of the water pipe, and the automatic water valve is arranged at one quarter of the bottom of the loading block.

[0014] As a preferred technical solution of the present application, a movable water plug is fixedly installed in the middle of the liquid outlet pipe, and the movable water plug is a bouncing water plug.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] In the scheme of this application:

[0017] 1. This utility model integrates the cultivation and collection of entomopathogenic nematodes, enabling the rapid acquisition of large quantities of entomopathogenic nematodes, meeting both scientific research and application needs. The simple operation procedure makes it suitable for the cultivation of entomopathogenic nematodes, significantly reducing operation time and labor costs. This utility model also boasts the advantages of simple operation, high efficiency, and high accuracy, making it a rational entomopathogenic nematode separation and collection device with significant application value and scientific significance in the cultivation and collection of entomopathogenic nematodes.

[0018] 2. This utility model utilizes a live separation method to effectively isolate entomopathogenic nematodes without affecting their activity. An insect-proof net installed above effectively prevents interference from other insects. A water replenisher maintains the water level in the culture container to a high degree, reducing the frequency of manual water additions. Compared to the White Trap method, it offers a larger capacity, faster collection, and a shorter collection time. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the three-dimensional structure of a simple entomopathogenic nematode culture and collection device provided by the present invention;

[0020] Figure 2 A schematic diagram of the internal three-dimensional structure of the simple entomopathogenic nematode cultivation and collection device provided by the utility model;

[0021] Figure 3 This is a schematic diagram of the cross-sectional structure of the simple entomopathogenic nematode cultivation and collection device provided by the utility model when viewed from the front.

[0022] Indicated in the figure:

[0023] 1. Culture container; 2. Support legs; 3. Loading block; 4. Water-absorbing filter paper; 5. Insect screen; 6. Liquid outlet pipe; 7. Collection container; 8. Water tank; 9. Support frame; 10. Water pipe; 11. Automatic water valve; 12. Movable water plug; 13. Fixed frame; 14. Installation frame. DETAILED DESCRIPTION

[0024] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be described clearly and completely in conjunction with the accompanying drawings. Obviously, the examples described are only part of the embodiments of the present invention, not all of the embodiments.

[0025] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but rather merely represents some embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0026] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions therein can be combined with each other.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0028] In the description of this utility model, it should be noted that the terms "upper" and "lower" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or the orientations or positional relationships in which the utility model product is typically placed when in use, or the orientations or positional relationships commonly understood by those skilled in the art. Such terms are intended solely to facilitate the description of this utility model and to simplify the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" and the like are used solely to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0029] In order to solve the technical problems in the background technology, the following simple entomopathogenic nematode culture and collection device is provided:

[0030] Combine Figure 1 - Figure 3As shown, the utility model provides a simple entomopathogenic nematode culture and collection device, including a culture container 1, a supporting leg 2 is fixedly installed on the lower surface of the culture container 1, and a loading block 3 is placed inside the culture container 1, the upper surface of the loading block 3 is covered with a layer of absorbent filter paper 4, and the size of the absorbent filter paper 4 is slightly larger than the loading block 3, and the excess part of the absorbent filter paper 4 is in contact with the lower bottom surface of the culture container 1, an insect-proof net 5 is provided on the top of the culture container 1, and a liquid outlet pipe 6 is fixedly installed on the bottom of the culture container 1, and a collection container 7 is provided on the lower side of the liquid outlet pipe 6.

[0031] In this embodiment: the lower surface of the culture container 1 is firmly installed with support legs 2 to ensure the stability of the device; a loading block 3 is set inside the culture container 1, which is covered with a layer of water-absorbing filter paper 4 that is slightly larger and whose edges can touch the lower bottom surface of the culture container 1, which not only ensures good water absorption performance but also facilitates the subsequent drainage of the nematode suspension; an insect-proof net 5 is set on the top of the culture container 1 to effectively prevent interference from other insects and ensure the purity of the culture environment; and the liquid outlet pipe 6 located at the bottom of the culture container 1 is tightly connected to the collection container 7 below, forming a smooth nematode suspension collection system, making the entire culture and collection process both efficient and convenient.

[0032] As a preferred embodiment, the bottom surface of the culture container 1 is provided with a slope inclined toward the liquid outlet pipe 6, and the object loading block 3 is provided in a rectangular parallelepiped.

[0033] In this embodiment: the bottom surface of the culture container 1 is designed to be a slope inclined toward the liquid outlet pipe 6, which can greatly promote the smooth discharge of the nematode suspension and avoid residue and accumulation; at the same time, the object block 3 adopts a rectangular shape, which is not only more stably supported in the culture container 1, but also its regular geometric shape is more conducive to the trapping and cultivation of entomopathogenic nematodes, and at the same time makes the cleaning and maintenance of the device easier and more efficient.

[0034] As a preferred embodiment, a fixing frame 13 is fixedly installed inside the top of the culture container 1 , and a mounting frame 14 is provided on the upper side of the fixing frame 13 , and the insect-proof net 5 is fixedly installed inside the mounting frame 14 .

[0035] In this embodiment, a combination structure of a fixing frame 13 and an installation frame 14 is provided inside the top of the culture container 1. The fixing frame 13 is installed inside the culture container 1 to provide solid support for the installation frame 14 above, and the installation frame 14 is used to fix the insect-proof net 5. This design facilitates the installation and removal of the insect-proof net 5 and ensures its stability and durability during use.

[0036] As a preferred embodiment, a water tank 8 is provided on the upper side of the culture container 1, and a support frame 9 is fixedly installed on the bottom of the water tank 8. A water pipe 10 is fixedly installed on the lower surface of the water tank 8, and the other end of the water pipe 10 extends into the interior of the culture container 1.

[0037] In this embodiment, a water tank 8 and its supporting components are added to the upper side of the culture container 1 to provide a stable water supply for the entire culture process. The water tank 8 is firmly installed in an appropriate position through a support frame 9, and the water pipe 10 connected to the bottom thereof cleverly extends to the interior of the culture container 1, realizing automatic water level regulation and replenishment. This design ensures the continuous stability of the water level in the culture container 1, effectively reduces the frequency of manual water addition, and improves the overall automation level and operational convenience of the culture collection device.

[0038] As a preferred embodiment, an automatic water valve 11 is fixedly installed at the other end of the water pipe 10 , and the automatic water valve 11 is arranged at one quarter of the bottom of the loading block 3 .

[0039] In this embodiment: an automatic water valve 11 is cleverly installed at the other end of the water pipe 10, and the automatic water valve 11 is precisely set at the position of one-quarter of the bottom of the carrier block 3; it realizes precise control of the water level inside the culture container 1, and can also automatically adjust the water volume according to actual needs, avoiding the adverse effects of excessively high or low water levels on the cultivation of insect pathogenic nematodes. The introduction of the automatic water valve 11 not only improves the intelligence level of the entire device, but also greatly simplifies the operation process, making the cultivation and collection work more efficient and accurate.

[0040] As a preferred embodiment, a movable water plug 12 is fixedly installed in the middle of the liquid outlet pipe 6, and the movable water plug 12 is a bouncing water plug.

[0041] In this embodiment, a movable water plug 12 is installed in the middle of the liquid outlet pipe 6, and the water plug is a spring-type water plug, which not only allows the user to easily and quickly control the opening and closing of the liquid outlet pipe 6, but also effectively prevents accidental leakage of liquid during the culture process, ensuring the stability and safety of the culture environment.

[0042] Specifically, the working principle of this solution is:

[0043] First, the specimen block 3 is placed inside the culture container 1 and covered with a slightly larger absorbent filter paper 4, the edge of which contacts the bottom surface of the culture container 1. Next, the entomopathogenic nematodes are placed on the absorbent filter paper 4 for cultivation. At this point, the insect-proof net 5 on the top of the culture container 1 is securely mounted using the combined structure of the mounting frame 14 and the fixing frame 13, effectively preventing interference from other insects.

[0044] To maintain humidity in the culture environment, a water tank 8 is mounted on the upper side of the culture container 1 via a support frame 9. Water is introduced into the culture container 1 via a water pipe 10. An automatic water valve 11 at the end of the water pipe 10 is located in the bottom quarter of the object carrier 3. It automatically adjusts the water flow according to actual demand to ensure a stable and appropriate water level in the culture container 1.

[0045] During the culture process, the entomopathogenic nematodes grow and reproduce on the absorbent filter paper 4, and the resulting nematode suspension permeates through the absorbent filter paper 4 to the bottom of the culture container 1. Because the bottom surface of the culture container 1 is designed to be sloped toward the liquid outlet pipe 6, the nematode suspension can flow smoothly toward the liquid outlet pipe 6.

[0046] When the nematode suspension needs to be collected, the spring-type movable water plug 12 in the middle of the liquid outlet pipe 6 is opened, and the nematode suspension can flow into the collection container 7 below through the liquid outlet pipe 6. After the collection is completed, the movable water plug 12 is closed to prevent the liquid in the culture container 1 from continuing to flow out.

[0047] The above is the working process of the entire device, and the contents not described in detail in this specification belong to the existing technology known to professional and technical personnel in this field.

[0048] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. Although this specification has described the present invention in detail with reference to the above embodiments, the present invention is not limited to the above specific implementation methods. Therefore, any modification or equivalent replacement of the present invention; and all technical solutions and improvements thereof that do not depart from the spirit and scope of the present invention are included in the scope of the claims of the present invention.

Claims

1. A simple entomopathogenic nematode culture and collection device, comprising a culture container (1), characterized in that: The lower surface of the culture container (1) is fixedly mounted with a support leg (2), and a loading block (3) is placed inside the culture container (1). The upper surface of the loading block (3) is covered with a layer of absorbent filter paper (4), and the size of the absorbent filter paper (4) is slightly larger than the loading block (3), and the excess portion of the absorbent filter paper (4) is in contact with the bottom surface of the culture container (1). The top of the culture container (1) is provided with an insect-proof net (5), and the bottom of the culture container (1) is fixedly mounted with a liquid outlet pipe (6), and a collection container (7) is provided on the lower side of the liquid outlet pipe (6).

2. A simple and convenient entomopathogenic nematode culture and collection device according to claim 1, characterized in that: The bottom surface of the culture container (1) is provided with a slope inclined toward the liquid outlet pipe (6), and the object loading block (3) is arranged in a rectangular parallelepiped.

3. A simple and convenient entomopathogenic nematode culture and collection device according to claim 1, characterized in that: A fixing frame (13) is fixedly installed inside the top of the culture container (1), and a mounting frame (14) is provided on the upper side of the fixing frame (13). The insect-proof net (5) is fixedly installed inside the mounting frame (14).

4. A simple and convenient entomopathogenic nematode culture and collection device according to claim 1, characterized in that: A water tank (8) is provided on the upper side of the culture container (1), and a support frame (9) is fixedly installed on the bottom of the water tank (8). A water pipe (10) is fixedly installed on the lower surface of the water tank (8), and the other end of the water pipe (10) extends into the interior of the culture container (1).

5. A simple and convenient entomopathogenic nematode culture and collection device according to claim 4, characterized in that: An automatic water valve (11) is fixedly mounted on the other end of the water delivery pipe (10), and the automatic water valve (11) is arranged at one quarter of the bottom of the object-carrying block (3).

6. The simple and convenient entomopathogenic nematode culture and collection device according to claim 1, characterized in that: A movable water plug (12) is fixedly installed in the middle of the liquid outlet pipe (6), and the movable water plug (12) is a bouncing water plug.

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