Cell recovery device

Abstract

The utility model discloses a cell resuscitation device, and particularly relates to the technical field of biomedical experimental equipment, which comprises a water bath kettle, a support plate mounted at the upper end of the water bath kettle, support rods mounted at the upper end of the support plate, a rack mounted between the two support rods, a sleeve mounted at the middle end of the top of the rack, and a threaded rod mounted at the output end of a rotating motor. A lifting plate is installed on the threaded rod in a threaded mode, the side end of the lifting plate is slidably connected with the inner wall of the sleeve through a sliding block, a connecting rod is installed at the bottom end of the lifting plate, an installing plate is fixed to the bottom end of the connecting rod, a rotating rod is connected to the output end of the servo motor, an upper disc is installed at the bottom end of the rotating rod, and a plurality of inserting holes are annularly and evenly formed in the upper disc. A connecting plate is mounted at the side end of the upper disc, a lower disc is mounted at the bottom of the side end of the connecting plate, and a plurality of positioning grooves are annularly and uniformly formed in the lower disc. According to the cryopreservation tube, the recovery of cells in warm water can be accelerated conveniently, a plurality of cryopreservation tubes can be operated at the same time, and the efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical experimental equipment, and more specifically, to a cell resuscitation device. Background Art

[0002] Cell experiments are indispensable in biomedical experiments. In order to conduct more in-depth research on the structural or biochemical characteristics of cells, it is often necessary to preserve cells at low temperatures for a long time. After freezing, these biological tissues can be studied as needed. The current common method is to store cell specimens in liquid nitrogen through cryopreservation tubes for low-temperature preservation of cells. Liquid nitrogen is the liquid form of nitrogen gas, and its boiling point at normal pressure is minus 196 degrees Celsius, so liquid nitrogen is often used as a refrigerant. Due to the inactive chemical properties of liquid nitrogen, it can directly contact biological cells and immediately freeze biological tissues without destroying biological activity. Therefore, it is often used to preserve cell specimens.

[0003] The initiation of cell experiments often requires the thawing of frozen cells for subsequent culture and passage. Currently, cell thawing relies on manual operation. The existing method of manual cell thawing is to tightly clamp the cryotube containing the cell specimen in a liquid nitrogen tube with tweezers, place the removed cryotube in a preheated 37°C water bath, and shake it vigorously back and forth for several minutes. When the cells in the cryotube are completely lysed, the cell thawing is complete. However, the following disadvantages exist in the process of manual cell thawing: 1. When manually thawing cells, the cryotube needs to be tightly clamped with tweezers and shaken rapidly in a 37°C water bath for several minutes to thaw the cells. This process can cause wrist fatigue in the experimenter; 2. When manually thawing cells, only one cryotube can be operated at a time. If multiple tubes of cells need to be thawed, the operation must be repeated several times, which is tedious and time-consuming; 3. When manually thawing cells, the prolonged and vigorous shaking often causes wrist fatigue in the operator, which can easily cause the cryotube to be submerged below the water surface, causing contamination and damage to the cells. Utility Model Content

[0004] In order to overcome the above-mentioned defects of the prior art, an embodiment of the present invention provides a cell resuscitation device to solve the problems raised in the above-mentioned background technology.

[0005] The top end face of described sliding panel also is provided with an interlocking structure, and the interlocking structure of described sliding panel also is provided with an interlocking structure, and the interlocking structure of described sliding panel is provided with an interlocking structure.

[0006] In a preferred embodiment, a limit block is installed at the bottom end of the threaded rod.

[0007] In a preferred embodiment, the lower disc is arranged directly below the upper disc, and the rotating rod, the upper disc and the lower disc are coaxially arranged.

[0008] In a preferred embodiment, the positioning grooves correspond to the insertion holes one by one.

[0009] In a preferred embodiment, the bottom end of the water bath is rectangular and fixedly mounted with four bases.

[0010] Compared with the prior art, the technical effects and advantages of this utility model are:

[0011] The utility model heats the water in the water bath to 37°C, inserts the cryopreservation tube from the insertion hole on the upper disc, and positions the bottom end of the cryopreservation tube in the positioning groove on the lower disc to prevent the cryopreservation tube from shaking. The upper disc is evenly provided with a plurality of insertion holes in a ring shape, and the lower disc is evenly provided with a plurality of positioning grooves in a ring shape, which is convenient for placing a plurality of cryopreservation tubes. The threaded rod is driven to rotate by the rotation of the rotary motor, and a lifting plate is threadedly installed on the threaded rod. The side end of the lifting plate is slidably connected to the inner wall of the sleeve through a slider. The threaded rod rotates to drive the lifting plate to move downward, and the downward movement of the lifting plate drives the connecting rod and the mounting plate to move downward, thereby driving the servo motor, the rotating rod, The upper disc, connecting plate and lower disc move downward, thereby driving the cryopreservation tubes placed on the upper disc and lower disc into the warm water in the water bath. The forward and reverse rotation of the servo motor drives the rotating rod to rotate forward and reverse, and the forward and reverse rotation of the rotating rod drives the upper disc to rotate forward and reverse. The upper disc drives the lower disc to rotate forward and reverse through the connecting plate, thereby driving the cryopreservation tubes placed on the upper disc and lower disc to rotate forward and reverse, which facilitates the acceleration of cell recovery in warm water. There is no need to manually shake the cryopreservation tubes, which makes it less likely for the experimenter's wrist to get tired. In addition, several cryopreservation tubes can be operated simultaneously, which shortens the operation time, reduces the labor intensity of the experimenter, and improves efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0013] Figure 2 It is a schematic diagram of the top structure of the upper disc 15 of the present invention.

[0014] The accompanying drawings are marked as follows: 1. water bath; 2. support plate; 3. support rod; 4. frame; 5. sleeve; 6. rotating motor; 7. threaded rod; 8. limit block; 9. lifting plate; 10. slider; 11. connecting rod; 12. mounting plate; 13. servo motor; 14. rotating rod; 15. upper disc; 16. jack; 17. connecting plate; 18. lower disc; 19. positioning groove; 20. base. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0016] according to Figure 1-2The cell recovery device shown in the figure includes a water bath 1, a support plate 2 is installed on the upper end of the water bath 1, a support rod 3 is installed on the upper end of the support plate 2, a frame 4 is installed between the two support rods 3, a sleeve 5 is installed at the top middle end of the frame 4, a rotating motor 6 is installed on the upper end of the sleeve 5, a threaded rod 7 is installed at the output end of the rotating motor 6, a lifting plate 9 is threadedly installed on the threaded rod 7, the side end of the lifting plate 9 is slidably connected to the inner wall of the sleeve 5 through a slider 10, and the bottom of the lifting plate 9 is connected to the inner wall of the sleeve 5 through a slider 10. A connecting rod 11 is installed at the end, a mounting plate 12 is fixed to the bottom end of the connecting rod 11, a servo motor 13 is installed at the upper end of the mounting plate 12, and the output end of the servo motor 13 is connected to a rotating rod 14, an upper disc 15 is installed at the bottom end of the rotating rod 14, a plurality of jacks 16 are evenly arranged in a ring shape on the upper disc 15, and a connecting plate 17 is installed at the side end of the upper disc 15, a lower disc 18 is installed at the bottom of the side end of the connecting plate 17, and a plurality of positioning grooves 19 are evenly arranged in a ring shape on the lower disc 18.

[0017] In a preferred embodiment, a limit block 8 is installed at the bottom end of the threaded rod 7 , and the limit block 8 is convenient for preventing the lifting plate 9 from falling off the threaded rod 7 .

[0018] In a preferred embodiment, the lower disc 18 is arranged directly below the upper disc 15, and the rotating rod 14, the upper disc 15, and the lower disc 18 are coaxially arranged, so that the rotating rod 14 rotates to drive the upper disc 15 and the lower disc 18 to rotate coaxially to prevent shaking.

[0019] In a preferred embodiment, the positioning groove 19 corresponds to the insertion hole 16 one by one, and the insertion hole 16 is convenient for inserting the cryotube. The bottom of the cryotube is positioned in the positioning groove 19 on the lower disc 18 to prevent the cryotube from shaking.

[0020] In a preferred embodiment, the bottom end of the water bath 1 is rectangular and fixedly mounted with four bases 20 to facilitate supporting the water bath 1 .

[0021] In summary, the present invention provides a cell recovery device. When in use, the water in the water bath 1 is heated to 37°C, and the cryopreservation tube is inserted from the socket 16 on the upper disc 15. The bottom end of the cryopreservation tube is positioned in the positioning groove 19 on the lower disc 18 to prevent the cryopreservation tube from shaking. A plurality of sockets 16 are evenly arranged in a ring shape on the upper disc 15, and a plurality of positioning grooves 19 are evenly arranged in a ring shape on the lower disc 18, which are convenient for placing multiple cryopreservation tubes. The threaded rod 7 is driven to rotate by the rotation of the rotary motor 6. A lifting plate 9 is threadedly installed on the threaded rod 7. The side end of the lifting plate 9 is slidably connected to the inner wall of the sleeve 5 through the slider 10. The threaded rod 7 rotates to drive the lifting plate 9 to move downward, and the lifting plate 9 moves downward to drive the connecting rod 11 and the mounting plate 12 to move downward, thereby bringing The servo motor 13, the rotating rod 14, the upper disc 15, the connecting plate 17 and the lower disc 18 are driven downward, thereby driving the cryopreservation tubes placed on the upper disc 15 and the lower disc 18 to enter the warm water in the water bath 1. The forward and reverse rotation of the servo motor 13 drives the rotating rod 14 to rotate forward and reverse, and the forward and reverse rotation of the rotating rod 14 drives the upper disc 15 to rotate forward and reverse. The upper disc 15 drives the lower disc 18 to rotate forward and reverse through the connecting plate 17, thereby driving the cryopreservation tubes placed on the upper disc 15 and the lower disc 18 to rotate forward and reverse, which is convenient for accelerating the recovery of cells in warm water. There is no need to manually shake the cryopreservation tubes, which makes it less likely for the experimenter's wrist to get tired. In addition, several cryopreservation tubes can be operated at the same time, which shortens the operation time, reduces the labor intensity of the experimenter, and improves efficiency.

[0022] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense, and may refer to mechanical or electrical connections, internal communication between two components, or direct connection. "Up," "down," "left," and "right" are only used to indicate relative positional relationships. When the absolute positions of the objects being described change, the relative positional relationships may also change.

[0023] Secondly: The drawings of the embodiments disclosed in this utility model only involve structures related to the embodiments disclosed in this utility model. Other structures can refer to common designs. In the absence of conflicts, the same embodiment and different embodiments of the utility model can be combined with each other.

[0024] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell recovery device, comprising a water bath (1), characterized in that: The upper end of the water bath (1) is provided with a support plate (2), the upper end of the support plate (2) is provided with a support rod (3), a frame (4) is provided between the two support rods (3), a sleeve (5) is provided at the top middle end of the frame (4), a rotating motor (6) is provided at the upper end of the sleeve (5), a threaded rod (7) is provided at the output end of the rotating motor (6), a lifting plate (9) is threadedly provided on the threaded rod (7), a side end of the lifting plate (9) is slidably connected to the inner wall of the sleeve (5) through a slider (10), and a connecting rod ( 11), a mounting plate (12) is fixed to the bottom end of the connecting rod (11), a servo motor (13) is installed on the upper end of the mounting plate (12), the output end of the servo motor (13) is connected to a rotating rod (14), an upper disc (15) is installed on the bottom end of the rotating rod (14), a plurality of jacks (16) are evenly arranged in an annular shape on the upper disc (15), and a connecting plate (17) is installed on the side end of the upper disc (15), a lower disc (18) is installed on the bottom end of the side end of the connecting plate (17), and a plurality of positioning grooves (19) are evenly arranged in an annular shape on the lower disc (18).

2. A cell resuscitation device according to claim 1, characterized in that: A limiting block (8) is installed at the bottom end of the threaded rod (7).

3. The cell resuscitation device according to claim 1, characterized in that: The lower disc (18) is arranged directly below the upper disc (15), and the rotating rod (14), the upper disc (15), and the lower disc (18) are coaxially arranged.

4. The cell resuscitation device according to claim 1, characterized in that: The positioning grooves (19) correspond to the insertion holes (16) in a one-to-one manner.

5. The cell resuscitation device according to claim 1, characterized in that: The bottom end of the water bath (1) is rectangular and fixedly mounted with four bases (20).

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