Portable small fluorescent quantitative PCR instrument

Abstract

The utility model discloses a portable small fluorescent quantitative PCR (polymerase chain reaction) instrument, which belongs to the field of medical instruments and comprises a cover body, a shell, a heating module and an amplification detection module, the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigeration sheet, a cooling sheet and a fan, and the amplification sample block is arranged in the shell. The amplification sample block is used for placing a PCR test tube, the amplification sample block and the cooling fin are detachably connected with the fan, and the refrigeration sheet is clamped between the amplification sample block and the cooling fin. The device is free of moving parts inside, small in size, light in weight, good in damping effect and convenient to carry; the requirement for a user is low, operation is easy, automatic detection is achieved when a power switch is turned on, additional operation is not needed, and the requirements of professionals and family use can be met at the same time; the heat dissipation effect is good, the heating sample block is small in size, the temperature circulation speed is high, the detection time is shortened, and rapid detection is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a portable small-scale fluorescence quantitative PCR instrument. Background Art

[0002] Fluorescence quantitative PCR (PCR) technology is a key tool in modern molecular biology, widely used in genetic testing, pathogen diagnosis, and gene expression analysis. Traditional fluorescence quantitative PCR instruments are typically bulky, complex, and demanding in their operating environment. While generally suitable for laboratory settings, their use is significantly limited in clinical field testing, field operations, and resource-constrained areas.

[0003] With the increasing popularity of molecular diagnostic technology, the market demand for portable, fast, and efficient fluorescence quantitative PCR instruments is growing. Mainstream multi-channel real-time fluorescence quantitative PCR instruments on the market are generally large and heavy, with complex optical and mechanical systems, and slow detection speeds, making them incapable of on-site testing anytime, anywhere. Utility Model Content

[0004] In order to overcome the shortcomings of the existing technology, the purpose of the utility model is to provide a portable small-scale fluorescent quantitative PCR instrument with simple operation, small size, light weight, and fast detection speed, which improves the use efficiency while meeting the accuracy requirements. It is not only convenient for professionals to use outside, but also suitable for individuals to purchase for home self-testing.

[0005] The purpose of this utility model is achieved by the following technical solutions:

[0006] A portable small-scale fluorescent quantitative PCR instrument includes a cover, a shell, a heating module, and an amplification detection module. The cover is movably connected to the shell, the heating module is arranged on the cover, and the amplification detection module is arranged in the shell. The amplification detection module includes an amplification sample block, a cooling fin, a heat sink, and a fan. The amplification sample block is used to place PCR test tubes. The amplification sample block and the heat sink are both detachably connected to the fan, and the cooling fin is clamped between the amplification sample block and the heat sink.

[0007] Furthermore, the heating module includes a heating sample block and a heating film. The heating sample block is detachably connected to the cover body, and the heating film is adhered to the side of the heating sample block.

[0008] Furthermore, the heating film is made of polyimide flexible heating film.

[0009] Furthermore, the heating sample block is provided with a mounting hole, and the mounting hole is used to install a PT100 platinum resistor with thermal conductive silicone.

[0010] Furthermore, the heating sample block is provided with a first through hole, an upper cover circuit board is mounted on the upper side of the heating sample block, the upper cover circuit board is used to control the temperature and the light source, and the first through hole provides an optical path for the excitation light.

[0011] Furthermore, the amplification sample block is provided with a test tube groove and a circular hole. The test tube groove is located at the upper and lower sides of the amplification sample block, and the circular hole is located on the side of the test tube groove. The circular hole is used to conduct the emitted light and fix the photodiode.

[0012] Furthermore, the amplification sample block is further provided with second through holes, the second through holes are located at both ends of the amplification sample block, and the second through holes are used to fix the fluorescence detection circuit board.

[0013] Furthermore, the cover body includes a top cover and a bottom cover, and the top cover and the bottom cover are detachably connected.

[0014] Furthermore, the shell includes a shell body and a base, and the shell body and the base are detachably connected.

[0015] Furthermore, the portable small-scale fluorescence quantitative PCR instrument also includes a screen, which is arranged on the shell and is communicatively connected with the heating module and the amplification detection module.

[0016] Compared with the existing technology, the portable small-scale fluorescent quantitative PCR instrument of the present invention includes a cover, a shell, a heating module, and an amplification detection module. The cover is movably connected to the shell, the heating module is arranged on the cover, and the amplification detection module is arranged in the shell. The amplification detection module includes an amplification sample block, a cooling plate, a heat sink and a fan. The amplification sample block is used to place PCR test tubes. The amplification sample block and the heat sink are both detachably connected to the fan, and the cooling plate is clamped between the amplification sample block and the heat sink. The present invention has no moving parts inside, is small in size, light in weight, has good shock absorption effect, and is easy to carry. It has low requirements for users and is simple to operate. It can automatically detect when the power switch is turned on without additional operation, and can meet the requirements of both professionals and families. It has good heat dissipation effect, a small size of the heating sample block, a fast temperature cycle speed, shortens the inspection time, and is convenient for rapid detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of the portable small-scale fluorescence quantitative PCR instrument of the utility model;

[0018] Figure 2 for Figure 1 Schematic diagram of the heating module of a portable small-scale fluorescence quantitative PCR instrument;

[0019] Figure 3 for Figure 1Schematic diagram of the structure of the amplification detection module of a portable small fluorescent quantitative PCR instrument.

[0020] In the figure: 10, cover body; 11, top cover; 12, bottom cover; 20, shell; 21, shell body; 211, heat dissipation hole; 22, base; 221, opening; 30, heating module; 301, heating sample block; 3011, first through hole; 3012, mounting hole; 302, heating film; 40, amplification detection module; 401, amplification sample block; 4011, test tube slot; 4012, round hole; 4013, second through hole; 402, cooling plate; 403, heat sink; 404, fan; 50, screen. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are for the purpose of describing specific embodiments only and are not intended to limit this invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0024] Figure 1-Figure 3 The portable small-sized fluorescence quantitative PCR instrument of the present invention comprises a cover 10 , a housing 20 , a heating module 30 , an amplification detection module 40 and a screen 50 .

[0025] In this embodiment:

[0026] The cover 10 includes a top cover 11 and a bottom cover 12, which are fixedly connected by threads. The bottom cover 12 is provided with a neodymium iron boron permanent magnet to ensure that the cover 10 and the housing 20 are firmly and reliably closed during operation. In this embodiment, the cover 10 is mounted to the housing 20 via a hinge.

[0027] The shell 20 includes a shell body 21 and a base 22. The shell body 21 and the base 22 are detachably fixedly connected. Neodymium iron boron permanent magnets are placed at the top of the upper side of the shell body 21 and the corresponding position of the bottom cover 12 to ensure that the cover body 10 and the shell 20 are firmly and reliably closed during operation. Heat dissipation holes 211 are opened on both sides of the shell body 21.

[0028] The base 22 is provided with threaded holes for fixing the main control board, and the sides are provided with openings 221 for fixing the power switch, TYPEC communication interface and DC power socket.

[0029] The heating module 30 consists of a heating block 301 and a heating film 302. The heating block 301 is bolted to the bottom cover 12. Two first through-holes 3011 are defined in the heating block 301, providing a path for the excitation light. A top cover circuit board is mounted on the top of the heating block 301, controlling both the temperature and the light source. A mounting hole 3012 with a diameter of 2 mm and a depth of 10 mm is also provided on the heating block 301. This hole accommodates a PT100 platinum resistor with thermally conductive silicone for temperature measurement.

[0030] The heating film 302 is attached to the side of the heating sample block 301. In this embodiment, the heating film 302 is made of a polyimide flexible heating film 302.

[0031] The amplification detection module 40 is screwed onto the upper interior side of the housing 21. The amplification detection module 40 includes an amplification sample block 401, a cooling fin 402, a heat sink 403, and a fan 404. In this embodiment, the amplification sample block 401, cooling fin 402, heat sink 403, and fan 404 are installed sequentially from top to bottom. The amplification detection module 40 is installed so that when the fan 404 blows toward the heat sink 403, the air flows toward the heat dissipation holes 211 on both sides of the housing 20.

[0032] The amplification block 401, the heat sink 403 and the fan 404 are connected by bolts. In this embodiment, the amplification block 401 is made of aluminum alloy, and the surface of the aluminum alloy is anodized to reduce the influence of external light sources on fluorescence detection.

[0033] The amplification sample block 401 has two test tube grooves 4011 of fixed depth, and a circular hole 4012 on the side for transmitting emitted light and fixing the photodiode. The amplification sample block 401 has second through holes 4013 at both ends for fixing the fluorescence detection circuit board.

[0034] The cooling plate 402 is coated with a layer of thermal conductive silicone grease and then clamped between the amplification sample block 401 and the heat sink 403. In this embodiment, the cooling plate 402 is made of semiconductor material.

[0035] The screen 50 is set on the shell 20 and is communicated with the heating module 30 and the amplification detection module 40. The screen 50 is fixed between the shell body 21 and the base 22 through internal buckles of the shell body 21 and the base 22. The base 22 and the shell body 21 are fixedly connected by threads.

[0036] In this application, the cover body 10 is movably connected to the shell 20, the heating module 30 is arranged on the cover body 10, and the amplification detection module 40 is arranged in the shell 20. The installation direction of the amplification detection module 40 is such that the fan 404 blows toward the heat sink 403, and the wind flow position is located at the position where the heat dissipation holes 211 are opened on both sides of the shell 20.

[0037] When the present application is in use, the sample and reagent are added to the PCR tube, the PCR instrument cover 10 is opened, the tube is placed in the amplification detection module 40, and the cover 10 is closed. The PCR instrument is tightly closed under the action of the permanent magnets in the cover 10 and the housing 20, and the upper side of the PCR tube is tightly attached to the heating module 30 to prevent the reagent from condensing on the tube cover during the temperature cycle; the switch located on the base 22 is turned on, and the screen 50 displays the temperature of the heating sample block 301, the temperature of the amplification sample block 401, the number of cycles and the fluorescence detection result. The heating module 30 starts working first, heats to a certain temperature and then remains constant. The amplification detection module 40 starts working, and the amplification sample block 401 undergoes temperature cycling under the action of the cooling plate 402. During the temperature cycling, when the temperature of the amplification sample block 401 rises, the fan 404 does not work. When the temperature drops, the fan 404 rotates at full speed. The temperature cycle ends when the amplification process is completed, the fluorescence detection circuit works, and the amplification result is displayed on the screen 50. This application has no moving parts inside, is small in size, light in weight, has good shock absorption effect, and is easy to carry; it has low requirements for users, is simple to operate, and automatically detects when the power switch is turned on without additional operation, which can meet the requirements of both professionals and home use; it has good heat dissipation effect, the heating sample block 301 is small in size, the temperature cycle speed is fast, the inspection time is shortened, and it is convenient for rapid detection.

[0038] The above embodiments merely represent several implementation methods of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that those skilled in the art will be able to make various modifications and improvements without departing from the spirit of the present invention. These modifications and improvements are equivalent to those made to the above embodiments based on the essential technology of the present invention and fall within the scope of protection of the present invention.

Claims

1. A portable small-scale fluorescence quantitative PCR instrument, characterized by: The amplification detection module comprises a cover, a shell, a heating module, and an amplification detection module. The cover is movably connected to the shell, the heating module is arranged on the cover, and the amplification detection module is arranged in the shell. The amplification detection module comprises an amplification sample block, a cooling fin, a heat sink, and a fan. The amplification sample block is used to place PCR test tubes. The amplification sample block and the heat sink are both detachably connected to the fan, and the cooling fin is clamped between the amplification sample block and the heat sink.

2. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The heating module includes a heating sample block and a heating film. The heating sample block is detachably connected to the cover body, and the heating film is adhered to the side of the heating sample block.

3. The portable small-scale fluorescence quantitative PCR instrument according to claim 2, characterized in that: The heating film is made of polyimide flexible heating film.

4. The portable small-scale fluorescence quantitative PCR instrument according to claim 2, characterized in that: The heating sample block is provided with a mounting hole, and the mounting hole is used to install a PT100 platinum resistor with thermal conductive silica gel.

5. The portable small-scale fluorescence quantitative PCR instrument according to claim 2, characterized in that: The heating sample block is provided with a first through hole. An upper cover circuit board is mounted on the upper side of the heating sample block. The upper cover circuit board is used to control the temperature and the light source. The first through hole provides an optical path for the excitation light.

6. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The amplification sample block is provided with a test tube groove and a circular hole. The test tube groove is located at the upper and lower sides of the amplification sample block, and the circular hole is located on the side of the test tube groove. The circular hole is used to conduct the emitted light and fix the photodiode.

7. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The amplification sample block is further provided with second through holes, which are located at both ends of the amplification sample block and are used to fix the fluorescence detection circuit board.

8. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The cover body includes a top cover and a bottom cover, and the top cover and the bottom cover are detachably connected.

9. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The shell comprises a shell body and a base, and the shell body and the base are detachably connected.

10. The portable small-scale fluorescence quantitative PCR instrument according to claim 1, characterized in that: The portable small-scale fluorescence quantitative PCR instrument further includes a screen, which is arranged on the housing and is communicatively connected with the heating module and the amplification detection module.

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