Positive pressure extraction device for sample pretreatment

Abstract

The utility model discloses a positive-pressure extraction device for sample pretreatment, and belongs to the technical field of positive-pressure extraction equipment. The positive pressure extraction device solves the problems that an existing positive pressure extraction device is low in pressure and unstable in flow velocity, sample liquid cannot be extracted at a constant speed, and the detection efficiency is too low by adopting a manual extraction mode. The device comprises a device housing, an extraction pressing plate, an extraction support, an extraction steel plate, a first lifting mechanism and a second lifting mechanism, the extraction support is arranged in the device housing, the extraction pressing plate is arranged above the extraction support, the extraction steel plate is arranged below the extraction support, the extraction pressing plate is arranged in the device housing through the first lifting mechanism, and the second lifting mechanism is arranged below the extraction steel plate. The extraction steel plate is installed in the equipment housing through a second lifting mechanism, a plurality of first extraction holes are machined in the extraction support, a plurality of second extraction holes are machined in the extraction steel plate, and the first extraction holes and the second extraction holes are coaxially formed in the longitudinal direction. According to the positive pressure extraction device, the extraction time of detection personnel is shortened, and the extraction efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of positive pressure extraction equipment, and specifically relates to a sample pre-treatment positive pressure extraction device. Background Art

[0002] Solid-phase extraction technology, as a purification process before analysis, has been widely used in the detection of various samples. According to the national standard "National Food Safety Standard - Determination of Lactoferrin in Food" (GB 5009.299-2024), samples containing lactoferrin are extracted with phosphate buffer, enriched, eluted, and purified by heparin affinity columns, separated by reversed-phase chromatography columns, and analyzed by liquid chromatography, which can accurately determine the lactoferrin content in the samples.

[0003] During sample processing using heparin affinity column extraction, testers typically connect the heparin affinity column via a syringe, first transferring the sample solution into the syringe, and manually pushing the syringe to allow the sample solution to flow through the heparin affinity column at a flow rate of 1ml / min to 3ml / min. Lactoferrin is adsorbed and enriched on the heparin affinity column, and finally eluted with phosphate sodium chloride buffer to achieve the purpose of sample purification. An investigation of positive pressure extraction devices on the market revealed that they generally use gas cylinders or peristaltic pumps to drive the sample filtrate through the extraction column. This results in low pressure and unstable flow rates, making it impossible to extract the sample solution at a uniform rate. Therefore, testers generally resort to manual extraction.

[0004] The extraction of lactoferrin using a heparin affinity column presents significant resistance and requires lengthy manual filtration. After draining the syringe, the extraction solution must be added again, and the heparin affinity column must be separated from the syringe, a complex process. As the number of samples tested increases, the labor intensity increases, while testing efficiency gradually decreases. Furthermore, existing research has not addressed the issue of separating the syringe from the heparin affinity column.

[0005] Therefore, the present application proposes a sample pre-treatment positive pressure extraction device to solve the above problems. Utility Model Content

[0006] The purpose of developing this utility model is to address the problems of existing positive pressure extraction devices, which suffer from low pressure and unstable flow rates, resulting in an inability to extract sample liquids at a uniform rate, and the low efficiency of manual extraction methods. The following is a brief overview of the utility model to provide a basic understanding of certain aspects of the utility model. It should be understood that this overview is not an exhaustive overview of the utility model. It is not intended to identify key or important aspects of the utility model, nor is it intended to limit the scope of the utility model.

[0007] The technical solution of this utility model:

[0008] A sample pretreatment positive pressure extraction device includes an equipment cover, an extraction pressure plate, an extraction bracket, an extraction steel plate, a first lifting mechanism and a second lifting mechanism. The extraction bracket is arranged in the equipment cover, the extraction pressure plate is arranged above the extraction bracket, and the extraction steel plate is arranged below the extraction bracket. The extraction pressure plate is installed in the equipment cover through the first lifting mechanism, and the extraction steel plate is installed in the equipment cover through the second lifting mechanism. A plurality of first extraction holes are processed on the extraction bracket, and a plurality of second extraction holes are processed on the extraction steel plate. The first extraction holes and the second extraction holes are arranged longitudinally coaxially.

[0009] Furthermore, the first lifting mechanism includes a first motor, a lead screw, a first slider, a first mounting frame, a first guide rod, a second mounting frame and a second slider. The first motor is mounted on the equipment cover, and the first mounting frame is mounted on the inner side of the equipment cover. A lead screw is rotatably arranged on the first mounting frame, one end of the lead screw passes through the first mounting frame and the equipment cover and is connected to the execution end of the first motor, the lead screw is cooperatively connected to the first slider, and a first guide rod is also provided on the first mounting frame. The first slider is slidably connected to the first guide rod, the first slider is connected to the side wall of the extraction pressure plate, the other side wall of the extraction pressure plate is connected to the second slider, the second slider is slidably connected to the second guide rod, the second guide rod is mounted on the second mounting frame, and the second mounting frame is mounted on the inner wall of the equipment cover. The first mounting frame and the second mounting frame are arranged in a mirror image.

[0010] Furthermore, the second lifting mechanism includes a second motor and a threaded rod. The second motor is installed at the bottom of the equipment cover. The execution end of the second motor passes through the equipment cover and is connected to the threaded rod. The threaded rod is arranged inside the equipment cover and is threadedly connected to the extraction steel plate. A guide column is provided on the inner bottom surface of the equipment cover, and the extraction steel plate is slidably connected to the guide column.

[0011] Furthermore, a front cover is hinged on the device cover, and a plurality of support legs are connected to the bottom of the device cover, the height of the support legs is greater than the height of the second motor, and slide grooves are symmetrically arranged on the inner side wall of the device cover, and the extraction bracket is slidably arranged in the device cover through the slide grooves.

[0012] Furthermore, the number of the first extraction holes is 6 to 9, and the diameter of the first extraction holes is 20 mm. The number of the second extraction holes is 6 to 9, and the diameter of the second extraction holes is 7 mm.

[0013] The utility model has the following beneficial effects:

[0014] 1. The present invention's sample pretreatment positive pressure extraction device reduces the extraction time for testers and improves work efficiency. Compared with traditional lactoferrin extraction, the extraction time of the present invention's extraction device is shorter. Through experiments, it was found that the extraction time of a manual syringe extraction of a sample is generally 35 minutes, while the extraction time of the present invention's extraction device is 15 minutes, saving more than half the time. In addition, testers can only extract two samples at a time, while the extraction device of the present invention can extract multiple samples at a time, greatly improving the work efficiency of testers.

[0015] 2. The utility model is a sample pretreatment positive pressure extraction device, which avoids the problem of excessive workload for testers. Traditional manual extraction of lactoferrin requires long-term continuous pressure, and the testers have to pull hard. After using this device, only the first motor needs to be started to extract lactoferrin. After extraction, the second motor is started to separate the syringe from the heparin affinity column, which significantly reduces the workload of the testers. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of a sample pre-treatment positive pressure extraction device;

[0017] Figure 2 It is the main view of the inside of the equipment cover;

[0018] Figure 3 Schematic diagram of the cooperation relationship between the extraction pressing plate and the first lifting mechanism;

[0019] Figure 4 It is a schematic diagram of the coordination relationship between the extraction steel plate and the second lifting mechanism.

[0020] In the figure: 1-equipment cover, 2-extraction pressure plate, 3-extraction bracket, 4-extraction steel plate, 5-first lifting mechanism, 6-second lifting mechanism, 11-support leg, 12-front cover, 13-chute, 31-first extraction hole, 41-second extraction hole, 42-guide column, 51-first motor, 52-screw, 53-first slider, 54-first mounting frame, 55-first guide rod, 56-second mounting frame, 57-second guide rod, 58-second slider, 61-second motor, 62-threaded rod. DETAILED DESCRIPTION

[0021] To make the objectives, technical solutions, and advantages of the present invention more clearly apparent, the present invention is described below using specific embodiments illustrated in the accompanying drawings. However, it should be understood that these descriptions are merely illustrative and are not intended to limit the scope of the present invention. Furthermore, descriptions of known structures and technologies are omitted in the following description to avoid unnecessary confusion regarding the concepts of the present invention.

[0022] The connections mentioned in this utility model are divided into fixed connections and detachable connections. The fixed connections (i.e., non-detachable connections) include but are not limited to conventional fixed connection methods such as hemming, rivet connection, bonding connection, and welding connection. The detachable connections include but are not limited to conventional detachable methods such as threaded connection, snap connection, pin connection, and hinge connection. When the specific connection method is not clearly specified, it is assumed that at least one connection method can always be found among the existing connection methods to achieve the function. Those skilled in the art can choose according to their needs. For example, a welded connection is selected for a fixed connection, and a hinge connection is selected for a detachable connection.

[0023] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, indirect connection through an intermediate medium, internal communication between two components, or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0024] Example 1, combined Figures 1-4 This embodiment describes a sample pretreatment positive pressure extraction device, comprising an equipment cover 1, an extraction pressure plate 2, an extraction bracket 3, an extraction steel plate 4, a first lifting mechanism 5 and a second lifting mechanism 6. The extraction bracket 3 is provided in the equipment cover 1, the extraction pressure plate 2 is arranged above the extraction bracket 3, and the extraction steel plate 4 is arranged below the extraction bracket 3. The extraction pressure plate 2 is installed in the equipment cover 1 through the first lifting mechanism 5, and the extraction steel plate 4 is installed in the equipment cover 1 through the second lifting mechanism 6. A plurality of first extraction holes 31 are processed on the extraction bracket 3, and a plurality of second extraction holes 41 are processed on the extraction steel plate 4. The first extraction holes 31 and the second extraction holes 41 are arranged longitudinally coaxially.

[0025] The number of the first extraction holes 31 is 6 to 9, and the diameter of the first extraction holes 31 is 20 mm. The number of the second extraction holes 41 is 6 to 9, and the diameter of the second extraction holes 41 is 7 mm. The syringe is mounted on the extraction bracket 3 through the first extraction holes 31, and the heparin affinity column or extraction column is mounted on the extraction steel plate 4 through the second extraction holes 41. The injection end of each syringe is connected to the heparin affinity column or extraction column respectively.

[0026] Before use, first turn on the second motor 61. After the second motor 61 is turned on, it drives the threaded rod 62 to rotate, and drives the extraction steel plate 4 threadedly connected to the threaded rod 62 to rise and fall to a suitable position. In this embodiment, 8 first extraction holes 31 are processed on the extraction bracket 3, and 8 second extraction holes 41 are correspondingly processed on the extraction steel plate 4. First, a heparin affinity column is placed in each second extraction hole 41, and then a syringe is placed in each first extraction hole 31, so that the injection end of the syringe is connected to the heparin affinity column. Then, the first motor 51 is turned on, and the first motor 51 drives the screw 52 to rotate. A nut is provided on the first slider 53, and the first slider 53 is connected to the screw 52 through the nut. When the lead screw 52 rotates forward, it drives the first slider 53 to move downward at a constant speed. The first guide rod 55 and the second guide rod 57 on the first mounting frame 54 and the second mounting frame 56 act as guides to ensure that the extraction plate 2 can move vertically up and down. The extraction plate 2 descends at a constant speed of 5 mm to 15 mm / min and presses down the syringe. The extract in each syringe flows through the heparin affinity column at a flow rate of 1 ml / min to 3 ml / min to complete the sample purification. The backs of the first mounting frame 54 and the second mounting frame 56 are both machined with multiple connecting holes. Screws pass through the connecting holes of the first mounting frame 54 and the second mounting frame 56 to fix them to the inner wall of the equipment cover 1;

[0027] After sample purification is completed, the second motor 61 is started, and the second motor 61 reverses to drive the threaded rod 62 to reverse, thereby driving the extraction steel plate 4 to move slowly downward, allowing the injection end of the syringe to separate from the upper end of the heparin affinity column for further sample filling.

[0028] A slide groove 13 is symmetrically processed on the inner wall of the equipment cover 1, and the extraction bracket 3 is slidably installed in the equipment cover 1 through the slide groove 13. The front of the equipment cover 1 is hinged with a front cover 12, and the front cover 12 is made of a transparent acrylic plate. The working status inside the equipment cover 1 can be viewed through the front cover 12. Four supporting legs 11 are connected to the bottom of the equipment cover 1. The height of the supporting legs 11 is greater than the height of the second motor 61 to prevent the second motor 61 from supporting the bottom.

[0029] This embodiment is only an illustrative description of the present application and does not limit its scope of protection. Those skilled in the art may also make partial changes to it. As long as they do not exceed the spirit of the present application, they are all within the scope of protection of the present application.

Claims

1. A sample pretreatment positive pressure extraction device, characterized in that: The invention comprises an equipment cover (1), an extraction pressure plate (2), an extraction support (3), an extraction steel plate (4), a first lifting mechanism (5) and a second lifting mechanism (6); an extraction support (3) is arranged in the equipment cover (1); an extraction pressure plate (2) is arranged above the extraction support (3); an extraction steel plate (4) is arranged below the extraction support (3); the extraction pressure plate (2) is installed in the equipment cover (1) through the first lifting mechanism (5); the extraction steel plate (4) is installed in the equipment cover (1) through the second lifting mechanism (6); a plurality of first extraction holes (31) are processed on the extraction support (3); a plurality of second extraction holes (41) are processed on the extraction steel plate (4); the first extraction holes (31) and the second extraction holes (41) are arranged coaxially in the longitudinal direction.

2. A sample pretreatment positive pressure extraction device according to claim 1, characterized in that: The first lifting mechanism (5) comprises a first motor (51), a lead screw (52), a first slider (53), a first mounting frame (54), a first guide rod (55), a second mounting frame (56) and a second slider (58), wherein the first motor (51) is mounted on the equipment cover (1), the first mounting frame (54) is mounted on the inner side of the equipment cover (1), a lead screw (52) is rotatably arranged on the first mounting frame (54), one end of the lead screw (52) passes through the first mounting frame (54) and the equipment cover (1) and is connected to the execution end of the first motor (51), and the lead screw (52) is connected to the first guide rod (55). The slider (53) is connected in a cooperative manner, and a first guide rod (55) is further provided on the first mounting frame (54). The first slider (53) is slidably connected to the first guide rod (55). The first slider (53) is connected to the side wall of the extraction pressure plate (2). The other side wall of the extraction pressure plate (2) is connected to the second slider (58). The second slider (58) is slidably connected to the second guide rod (57). The second guide rod (57) is mounted on the second mounting frame (56). The second mounting frame (56) is mounted on the inner wall of the equipment cover (1). The first mounting frame (54) and the second mounting frame (56) are arranged in a mirror image.

3. A sample pretreatment positive pressure extraction device according to claim 2, characterized in that: The second lifting mechanism (6) includes a second motor (61) and a threaded rod (62). The second motor (61) is installed at the bottom of the equipment cover (1). The execution end of the second motor (61) passes through the equipment cover (1) and is connected to the threaded rod (62). The threaded rod (62) is arranged inside the equipment cover (1) and is threadedly connected to the extraction steel plate (4). A guide column (42) is provided on the inner bottom surface of the equipment cover (1). The extraction steel plate (4) is slidably connected to the guide column (42).

4. The positive pressure extraction device for sample pretreatment according to claim 3, characterized in that: The device cover (1) is hinged with a front cover (12), the bottom of the device cover (1) is connected to a plurality of support legs (11), the height of the support legs (11) is greater than the height of the second motor (61), and the inner side wall of the device cover (1) is symmetrically provided with sliding grooves (13), and the extraction bracket (3) is slidably arranged in the device cover (1) through the sliding grooves (13).

5. The positive pressure extraction device for sample pretreatment according to claim 1, characterized in that: The number of the first extraction holes (31) is 6 to 9, and the diameter of the first extraction holes (31) is 20 mm. The number of the second extraction holes (41) is 6 to 9, and the diameter of the second extraction holes (41) is 7 mm.

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