Platelet aggregation function detection disc

Abstract

The utility model discloses a platelet aggregation function detection disc, which comprises a disc body and at least one microfluidic structure arranged on the disc body, the microfluidic structure comprises a sample adding groove, a separation groove, a precipitation groove and a detection groove which are sequentially arranged from the rotating center of the disc body to the periphery, a sample adding hole for liquid to enter is formed in the sample adding groove, the separation groove is connected with the sample adding groove and is connected with the detection groove through a first micro-channel, and the precipitation groove is connected with the separation groove. By adopting the disc, the separation of whole blood and the detection of the platelet aggregation function can be realized.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical detection, in particular to a platelet aggregation function detection disc. Background Art

[0002] In the existing technology, during the platelet aggregation function test, the separation of whole blood samples is done by using the centrifugal force generated by the high-speed rotation of the centrifuge to separate the plasma and blood cells with different specific gravities in the test tube, and then the test is performed. Due to the separation of the centrifugation and the test process, the test time is relatively long, and there is a possibility of sample contamination in the middle.

[0003] With the rapid development of biochemical testing, related separation technologies are also becoming increasingly advanced. For example, centrifugal microfluidics, which utilizes microfluidic structures to separate samples, is widely known. However, separation is only the foundation of testing. Rapid platelet aggregation testing using centrifugal microfluidics remains a key challenge in this field. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a platelet aggregation function detection disc, which can be used to separate whole blood and detect platelet aggregation function.

[0005] In order to solve the above problems, the solution adopted by the present invention is as follows:

[0006] A platelet aggregation function detection disc includes a disc body and at least one microfluidic structure arranged on the disc body. The microfluidic structure includes a sample loading groove, a separation groove, a sedimentation groove and a detection groove arranged in sequence from the rotation center of the disc body to the periphery. The sample loading groove is provided with a sample loading hole for liquid to enter. The separation groove is connected to the sample loading groove and is connected to the detection groove through a first microchannel. The sedimentation groove is connected to the separation groove.

[0007] Preferably, the disk body is a circular disk or a sector-shaped disk.

[0008] Preferably, the separation tank includes at least one reflux structure.

[0009] Preferably, the first microchannel is bent away from the separation groove to form a bent portion.

[0010] Preferably, the microfluidic structure further includes a certain number of components.

[0011] Preferably, the quantitative component is connected to the separation tank and includes a liquid storage tank, and the liquid storage tank is connected to the separation tank through a flow channel.

[0012] Preferably, the quantitative component is connected between the separation tank and the detection tank, and includes a diversion channel, a certain amount tank and a waste liquid tank. One end of the diversion channel is connected to the first microchannel, and the other end is connected to the waste liquid tank. One end of the quantitative tank is connected to the middle of the diversion channel, and the other end is connected to the detection tank.

[0013] Preferably, the detection tank includes a detection part and a storage part which are sequentially arranged along the rotation center of the disk toward the periphery.

[0014] Preferably, the storage part and the detection part are connected via a second microchannel.

[0015] Preferably, a plurality of capillaries are connected in the radial direction of the second microchannel.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] The detection disc of this utility model can simultaneously separate whole blood samples and detect platelet aggregation function, greatly shortening the detection time, improving detection efficiency, and reducing the possibility of sample contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a structural diagram of a platelet aggregation function detection disc in a preferred embodiment of the present invention;

[0019] Figure 2 yes Figure 1 Schematic diagram of the microfluidic structure;

[0020] Figure 3 is a schematic structural diagram of a microfluidic structure in a specific embodiment;

[0021] Figure 4 is a schematic structural diagram of a microfluidic structure in another specific embodiment;

[0022] Figure 5-6 Schematic diagram of the structure of the detection tank in different embodiments;

[0023] Among them, the figure marks are explained: 1 is the disc body, 2 is the microfluidic structure, 21 is the sample loading slot, 211 is the sample loading hole, 212 is the first air hole, 22 is the separation slot, 221 is the reflux structure, 222 is the first flow channel, 2220 is the bending part, 23 is the sedimentation tank, 24a is the liquid storage tank, 241b is the diversion channel, 242b is the quantitative tank, 243b is the waste liquid tank, 25 is the detection tank, 251 is the detection part, 252 is the storage part, 253 is the second microfluidic channel, and 26 is the reagent tank. DETAILED DESCRIPTION

[0024] The present invention will be described in further detail below with reference to the accompanying drawings.

[0025] Reference Figure 1-2 The present invention is a platelet aggregation function detection disc in a preferred embodiment, comprising a disc body 1 and at least one microfluidic structure 2 disposed on the disc body 1. The microfluidic structure 2 comprises a sample loading groove 21, a separation groove 22, a sedimentation groove 23, and a detection groove 25, arranged in sequence from the rotation center of the disc body 1 toward the periphery. The sample loading groove 21 is used to add a whole blood sample to the microfluidic structure 2 and is provided with a sample loading hole 211 for the whole blood sample to enter. The separation groove 22 is connected to the sample loading groove 21 and is connected to the detection groove 25 via a first microchannel 222. The sedimentation groove 23 is connected to the separation groove 22. In addition to centrifugal force, the whole blood sample is separated in the separation groove 22. Under the action of centrifugal force, blood cells enter the sedimentation groove 23, while plasma enters the detection groove 25 through the first microchannel 222. Platelet aggregation function is detected in the detection groove 25 by turbidimetry.

[0026] Furthermore, depending on the applied platform and the amount of sample to be tested, the disc body 1 may have different shapes, preferably a circular disc or a sector-shaped disc.

[0027] Furthermore, a first air hole 212 is provided on the detection tank 21 for balancing the air pressure in the sample loading tank 21 when loading a sample.

[0028] Furthermore, the separation tank 22 includes at least one reflux structure 221. This structure can be heart-shaped or have other symmetrical curved edges, without limitation. The reflux structure 221 primarily regulates liquid flow. Specifically, when the liquid flows to the periphery of the separation tank 22 due to centrifugal force simulating gravity, the shape of the reflux structure 221 guides the liquid downward, creating a single direction of flow, thereby regulating the liquid's flow.

[0029] Furthermore, the first microchannel 222 bends away from the separation tank 22 to form a bend 2220. The bend 2220 prevents liquid from prematurely passing through the first microchannel 222. For example, when the disk 1 is in operation, the liquid is retained in the first microchannel 222 due to capillary action. Only when the rotational speed of the disk 1 is increased so that the centrifugal force exceeds the surface tension of the liquid, does the liquid, driven by the angular velocity of the rotational speed, rise through the bend 2220 and enter the detection tank 25.

[0030] Reference Figure 3 , a schematic diagram of the microfluidic structure in a specific embodiment of the present invention. Microfluidic structure 2 includes a quantitative component. The quantitative component is connected to separation tank 22 and includes a liquid reservoir 24a. Liquid reservoir 24a is connected to separation tank 22 via a flow channel. After the whole blood sample enters separation tank 21, any sample that exceeds the flow channel height enters liquid reservoir 24a through the flow channel, thereby ensuring the quantitative concentration of the whole blood sample within separation tank 22.

[0031] Reference Figure 4 Specifically, the following is a schematic diagram of the structure of a microfluidic structure in another specific embodiment of the present invention. The microfluidic structure 2 includes a quantitative component, which is connected between the separation tank 22 and the detection tank 25. It includes a diverter channel 241b, a quantitative tank 242b, and a waste liquid tank 243b. One end of the diverter channel 241b is connected to the first microfluidic channel 222, and the other end is connected to the waste liquid tank 243b. One end of the quantitative tank 242b is connected to the middle of the diverter channel 241b, and the other end is connected to the detection tank 25. That is, the separated plasma is first quantified in the quantitative tank 242b, and then enters the detection tank 25 for detection.

[0032] Specifically, refer to Figure 2-6 According to the different functions that can be realized in different areas of the detection slot 25, the detection slot 25 includes a detection portion 251 and a storage portion 252 arranged in sequence from the rotation center of the disk body 1 to the outer periphery. The detection portion 251 and the storage portion 252 can be as follows Figure 2-4 As shown in the same chamber, it can also be Figure 5-6 As shown in FIG, each occupies a different chamber, and the two chambers are connected by a second microchannel 253. Furthermore, a plurality of capillaries are connected radially on the second microchannel 253 to increase the capillary force at the second microchannel 253 and further enhance the controllability of liquid flow.

[0033] Further, refer again Figure 2-4 The detection tank 25 is connected to a reagent tank 26 for adding reagents into the detection tank 25 .

[0034] Below Figure 3 The detection tank process is briefly described using the microfluidic structure 2 as an example:

[0035] S101. A whole blood sample is added to the sample loading tank 21 through the loading hole 211;

[0036] S102. The whole blood sample is transported to the separation tank by centrifugal drive, and the excess whole blood sample is distributed to the reservoir to achieve quantification of the whole blood sample in the separation tank;

[0037] S103 continues centrifugation, the blood cells are transported to the sedimentation tank, and the plasma is transported to the detection tank through the first microchannel;

[0038] S104 detects the transmittance value of PRP in the detection unit, and then adds reagents to the detection tank through the reagent tank, and after centrifugation, the transmittance value of PPP is measured at the detection unit;

[0039] S105. Obtain platelet aggregation function test results through changes in light transmittance.

[0040] The embodiments of this specific implementation method are all preferred embodiments of the present utility model, and are not intended to limit the scope of protection of the present utility model. Therefore, any equivalent changes made based on the structure, shape, and principle of the present utility model should be included in the scope of protection of the present utility model.

Claims

1. A platelet aggregation function detection disc, characterized in that: The invention comprises a disc body and at least one microfluidic structure arranged on the disc body, wherein the microfluidic structure comprises a sample loading groove, a separation groove, a precipitation groove and a detection groove arranged in sequence from the rotation center of the disc body to the periphery. The sample loading groove is provided with a sample loading hole for liquid to enter. The separation groove is connected to the sample loading groove and is connected to the detection groove through a first microchannel. The precipitation groove is connected to the separation groove.

2. The platelet aggregation function detection disc according to claim 1, characterized in that: The disk body is a circular disk or a sector-shaped disk.

3. The platelet aggregation function detection disc according to claim 1, wherein: The separation tank includes at least one reflux structure.

4. The platelet aggregation function detection disc according to claim 1, wherein: The first microchannel is bent away from the separation slot to form a bent portion.

5. The platelet aggregation function detection disc according to claim 1, wherein: The microfluidic structure further includes a number of components.

6. The platelet aggregation function detection disc according to claim 5, characterized in that: The quantitative component is connected to the separation tank and includes a liquid storage tank. The liquid storage tank is connected to the separation tank through a flow channel.

7. The platelet aggregation function detection disc according to claim 5, characterized in that: The quantitative component is connected between the separation tank and the detection tank, and includes a diversion channel, a certain amount tank and a waste liquid tank. One end of the diversion channel is connected to the first microchannel, and the other end is connected to the waste liquid tank. One end of the quantitative tank is connected to the middle of the diversion channel, and the other end is connected to the detection tank.

8. The platelet aggregation function detection disc according to any one of claims 1 to 7, characterized in that: The detection tank includes a detection part and a storage part which are sequentially arranged along the rotation center of the disk body toward the outer periphery.

9. The platelet aggregation function detection disc according to claim 8, characterized in that: The storage part and the detection part are connected via a second microchannel.

10. The platelet aggregation function detection disc according to claim 9, characterized in that: A plurality of capillaries are connected in a radial direction on the second microchannel.

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