Whole blood detection device integrated with self-negative pressure and surface modification flow aiding structure

Abstract

The embodiment of the utility model discloses a whole blood detection device integrated with a self-negative-pressure and surface-modified flow aiding structure, relates to the technical field of biological detection, and can effectively improve the detection efficiency of a whole blood sample. The device comprises a plate piece, a sealing piece and a filtering piece, the plate is provided with a containing cavity, one side of the plate is further provided with a sample adding opening, the sample adding opening is communicated with the containing cavity, a detection agent is arranged in the containing cavity, and a flow assisting structure is further arranged in the containing cavity and used for guiding flow of an object to be detected; the sealing piece plugs the sample adding opening; the filtering piece is arranged in a space defined by the sealing piece and the plate piece, a collecting cavity is formed between the filtering piece and the sealing piece, and the air pressure in the collecting cavity is lower than the atmospheric pressure. The method is suitable for the scene of rapid blood detection.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a whole blood detection device integrated with self-pressure and surface modification flow assisting structure. BACKGROUND

[0002] When detecting a whole blood sample, the common detection objects include serum or red blood cells, etc., which are usually obtained through complicated blood processing steps from the whole blood sample, and the time consumption is relatively long. However, the requirement for rapid blood detection in the clinic is increasing, especially in departments such as emergency department and ICU, which requires that the detection report can be provided within a short time after blood sampling. CONTENT OF THE INVENTION

[0003] Therefore, the present application provides a whole blood detection device integrated with self-pressure and surface modification flow assisting structure to improve the detection efficiency of whole blood samples.

[0004] In a first aspect, the present application provides a whole blood detection device integrated with self-pressure and surface modification flow assisting structure, comprising: a plate member, the plate member is provided with a containing cavity, one side of the plate member is further provided with a sample adding port, the sample adding port is in communication with the containing cavity, a detection agent is arranged in the containing cavity, and a flow assisting structure is further arranged in the containing cavity, and the flow assisting structure is used for guiding the flow of a to-be-detected object; a sealing member, the sealing member blocks the sample adding port; a filter member, the filter member is arranged in a space surrounded by the sealing member and the plate member, and a collection cavity is formed between the filter member and the sealing member, and the air pressure in the collection cavity is lower than the atmospheric pressure.

[0005] In a specific embodiment, the containing cavity comprises a main cavity and at least one sub-cavity, the sub-cavity is in communication with the main cavity, and the detection agent is arranged in the sub-cavity.

[0006] In a specific embodiment, the flow assisting structure is arranged on the inner wall of the containing cavity, and the flow assisting structure has hydrophilicity.

[0007] In a specific embodiment, the plate member comprises an intermediate plate body, an upper plate body and a lower plate body, the intermediate plate body is located between the upper plate body and the lower plate body, a first adhesive layer is arranged between the intermediate plate body and the upper plate body, a second adhesive layer is arranged between the intermediate plate body and the lower plate body, and the sample adding port is arranged on the upper plate body.

[0008] In a specific embodiment, the sealing member is in a groove shape, the groove opening of the sealing member faces the plate member, the groove bottom of the sealing member is away from the plate body, and the filter member is arranged at the groove opening of the sealing member.

[0009] In a specific embodiment, the filter is in the form of a groove, and the filter groove opening faces the seal, and the filter groove bottom extends into the holding cavity.

[0010] In a specific embodiment, the plate is at least partially made of a transparent material, and / or the seal is made of a transparent material.

[0011] In a specific embodiment, the filter is made of a filter membrane with a pore diameter of no more than 1 μm.

[0012] In a specific embodiment, the flow-aiding structure is subjected to at least one of the following treatments: oxygen plasma surface modification treatment, vacuum treatment, and surface growth of hydrophilic groups, including hydroxyl groups.

[0013] In a second aspect, the embodiments of the present application provide a detection method of a whole blood detection device integrated with a self-negative pressure and surface-modified flow-aiding structure, comprising the following steps: piercing the seal with a needle head with a whole blood sample, so that the whole blood sample is separated from the needle head and enters the collection cavity under the action of the negative pressure of the collection cavity, and the whole blood sample passes through the filter, and the object to be detected in the whole blood sample passes through the filter and enters the holding cavity, and the object to be detected is guided to the position of the detection agent under the guiding action of the flow-aiding structure on the inner wall of the holding cavity; and determining the detection result of the whole blood sample according to the reaction result of the object to be detected and the detection agent.

[0014] The embodiments of the present application provide a whole blood detection device integrated with a self-negative pressure and surface-modified flow-aiding structure, which comprises a plate, a seal, and a filter. The plate is provided with a holding cavity, and one side of the plate is further provided with a sample adding port which is in communication with the holding cavity. A detection agent is arranged in the holding cavity, and a flow-aiding structure is further arranged in the holding cavity. The flow-aiding structure is used for guiding the object to be detected. The seal blocks the sample adding port. The filter is arranged in the space surrounded by the seal and the plate, and a collection cavity is formed between the filter and the seal. The air pressure in the collection cavity is lower than the atmospheric pressure, so that a negative pressure lower than the atmospheric pressure is formed. The whole blood sample in the needle tube which pierces the seal can be quickly added to the collection cavity, and the object to be detected in the whole blood sample can pass through the filter. The flow-aiding structure in the holding cavity guides the object to be detected to the detection agent for rapid detection, so that the detection efficiency of the whole blood sample is effectively improved. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0016] Figure 1 A schematic diagram of a whole blood testing device integrating self-negative pressure and surface-modified flow-aiding structure provided in this application embodiment;

[0017] Figure 2 This is a schematic diagram of a whole blood testing device integrating self-negative pressure and surface-modified flow-aiding structure, provided as an embodiment of this application.

[0018] Explanation of key figure labels:

[0019] 100-Whole blood testing device; 10-Plate; 101-Sample dispensing port; 102-Receiving cavity; 1020-Main cavity; 1021-Sub-cavity; 103-Intermediate plate; 104-Upper plate; 105-Lower plate; 106-First adhesive layer; 107-Second adhesive layer; 20-Sealing element; 30-Filter element; 40-Needle. Detailed Implementation

[0020] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0021] It should be understood that the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0022] Traditional whole blood sample testing is cumbersome and time-consuming, while clinical demands for rapid blood testing are increasing. To address this issue, firstly, such as... Figure 1 As shown, an embodiment of the present invention provides a whole blood testing device 100 integrating self-negative pressure and surface-modified flow-aiding structure. The device may include: plate 10, sealing element 20, and filter element 30.

[0023] The plate 10 is provided with a receiving cavity 102, and a sample dispensing port 101 is also provided on one side of the plate 10. The sample dispensing port 101 is connected to the receiving cavity 102, which facilitates the addition of whole blood samples through the sample dispensing port 101. The receiving cavity 102 is provided with a detection reagent, which is used to detect the test object in the whole blood sample. The detection reagent can be solidified in a preset position in the receiving cavity 102. The receiving cavity 102 is also provided with a flow aid structure, which is used to guide the test object. For example, when the test object in the whole blood sample is serum, the flow aid structure can be used to quickly guide the serum to the position of the detection reagent in the receiving cavity 102, further improving the detection speed.

[0024] The sealing member 20 seals the sample adding port 101, thereby protecting the detection agent in the accommodating cavity 102 of the plate body from biological contamination, and facilitating the formation of a preset air pressure in the space surrounded by the sealing member 20 and the plate member 10, for example, the preset air pressure can be lower than the atmospheric pressure, thereby forming a negative pressure environment. The filter member 30 is arranged in the space surrounded by the sealing member 20 and the plate member 10, and a collection cavity is formed between the filter member 30 and the sealing member 20, the air pressure in the collection cavity is lower than the atmospheric pressure, and the filter member 30 is used to filter the whole blood sample added into the collection cavity, so that the target object can pass through the filter member 30 and be guided to the location of the detection agent by the flow assisting structure in the accommodating cavity 102. When the needle tube 40 pierces the sealing member 20 to add the whole blood sample into the collection cavity, in order to facilitate the rapid falling of the whole blood sample from the needle tube 40 into the collection cavity, in this embodiment, the air pressure in the collection cavity is lower than the atmospheric pressure of the environment in which the device is used, so that a negative pressure effect can be formed in the collection cavity, so that the whole blood sample can quickly separate from the needle tube 40 and be added into the collection cavity under the pressure difference between the collection cavity and the atmospheric pressure of the environment, thereby improving the use efficiency of the device and the detection efficiency and the detection efficiency of the whole blood sample. In addition, the air pressure in the accommodating cavity 102 can also be set to be lower than the atmospheric pressure; for example, the air pressure in the accommodating cavity 102 can be the same as the pressure in the collection cavity, so as to facilitate production and processing, and not to affect the filtering speed of the filter member 30; in some embodiments, when the filter member 30 has air pressure isolation capability, the air pressure in the accommodating cavity 102 can also be lower than the pressure in the collection cavity, so that the target object in the whole blood sample can pass through the filter member 30 more quickly under the pressure difference between the collection cavity and the accommodating cavity 102, thereby further improving the filtering speed of the filter member 30.

[0025] The whole blood detection device 100 provided by the embodiment of the present application integrates the self-negative pressure and the flow assisting structure with surface modification, and comprises a plate member 10, a sealing member 20 and a filter member 30. The plate member 10 is provided with an accommodating cavity 102, and one side of the plate member 10 is further provided with a sample adding port 101 which is in communication with the accommodating cavity 102. The accommodating cavity 102 is provided with a detection agent, and further provided with a flow assisting structure which is used to guide the target object. The sealing member 20 seals the sample adding port 101. The filter member 30 is arranged in the space surrounded by the sealing member 20 and the plate member 10, and a collection cavity is formed between the filter member 30 and the sealing member 20. The air pressure in the collection cavity is lower than the atmospheric pressure, thereby forming a negative pressure lower than the atmospheric pressure. The whole blood sample of the needle tube 40 which pierces the sealing member 20 can be quickly added into the collection cavity, and the target object in the whole blood sample can pass through the filter member 30. The flow assisting structure in the accommodating cavity 102 guides the target object to the detection agent for rapid detection, thereby effectively improving the detection efficiency of the whole blood sample.

[0026] To improve the detection capability of the whole blood detection device 100, so that a plurality of blood indexes can be detected at one time, optionally, in an embodiment of the present application, the accommodation cavity 102 comprises a main cavity 1020 and at least one sub-cavity 1021, the sub-cavity 1021 is in communication with the main cavity 1020, and the detection agent is arranged in the sub-cavity 1021. For example, a plurality of sub-cavities 1021 can be arranged in the accommodation cavity 102, and the plurality of sub-cavities 1021 surround the main cavity 1020. After being filtered by the filter 30, the to-be-detected objects in the whole blood sample enter the main cavity 1020, and then flow to the plurality of sub-cavities 1021 of the accommodation cavity 102 through the flow guiding of the flow guiding structure. Different detection agents with different detection functions can be arranged in each sub-cavity 1021, so that a plurality of blood indexes can be detected at one time in the whole blood detection operation.

[0027] Optionally, in an embodiment of the present application, the flow guiding structure is arranged on the inner wall of the accommodation cavity 102, and the flow guiding structure has hydrophilicity. Optionally, in an embodiment of the present application, the flow guiding structure is subjected to at least one of the following treatment processes: oxygen plasma surface modification treatment, vacuum treatment, surface growth of hydrophilic groups, the hydrophilic groups including hydroxyl groups or other groups with hydrophilicity, etc. to ensure that the flow guiding structure arranged on the inner wall of the accommodation cavity is subjected to hydrophilic treatment and has a better flow guiding effect. The flow guiding structure is arranged on the inner wall of the accommodation cavity 102, and the surface of the flow guiding structure is modified, for example, the flow guiding structure can be a flow guiding layer arranged on the inner wall of the accommodation cavity 102. In this way, not only the device structure is simplified, but also the to-be-detected objects in the whole blood sample can flow quickly in the accommodation cavity 102 and be smoothly guided to the positions of the detection agents.

[0028] To further improve the processability of the whole blood detection device 100 and reduce the production cost, optionally, in an embodiment of the present application, the plate member 10 comprises a middle plate body 103, an upper plate body 104 and a lower plate body 105, the middle plate body 103 is located between the upper plate body 104 and the lower plate body 105, a first adhesive layer 106 is arranged between the middle plate body 103 and the upper plate body 104, and a second adhesive layer 107 is arranged between the middle plate body 103 and the lower plate body 105. The sample adding port 101 is arranged on the upper plate body 104. The split structure facilitates the processing of the accommodation cavity 102 and the arrangement of the flow guiding structure on the inner wall of the accommodation cavity 102. For example, a through cavity can be formed in the middle plate body 103 and extend through the upper side and the lower side. The flow guiding structure is arranged on the inner wall of the through cavity. The upper plate body 104 is connected to the upper side of the middle plate body 103 by the first adhesive layer 106, and the lower plate body 105 is connected to the lower side of the middle plate body 103 by the second adhesive layer 107. The upper plate body 104, the middle plate body 103 and the lower plate body 105 surround the accommodation cavity 102. It can be seen that the structure simplifies the processing technology and is conducive to reducing the use cost of the whole blood detection device 100.

[0029] Optionally, in an embodiment of the present application, the sealing member 20 is in a groove shape, and the groove opening of the sealing member 20 faces the plate member 10, and the groove bottom of the sealing member 20 faces away from the plate body, and the filter member 30 is arranged in the groove opening of the sealing member 20. As shown in Figure 1 the groove opening of the groove-shaped sealing member 20 is buckled on one side of the plate member 10 and blocks the sample adding port 101, and the filter member 30 is arranged in the groove opening of the sealing member 20, which facilitates processing and assembly and is conducive to further improving the economy of the whole blood detection device 100.

[0030] Optionally, in an embodiment of the present application, the filter member 30 is in a groove shape, and the groove opening of the filter member 30 faces the sealing member 20, and the groove bottom of the filter member 30 extends into the containing cavity 102. The groove bottom of the groove-shaped filter member 30 (not shown in the figure) extends into the containing cavity 102, so that sufficient opposite surfaces can be formed between the groove wall of the filter member 30 and the inner wall of the containing cavity 102, and the opposite surfaces of the two can have a preset interval or can be in contact, so that after the whole blood sample is filtered by the filter member 30, the object to be detected that passes through the filter member 30 can be promptly and quickly guided by the flow assisting structure arranged on the inner wall of the containing cavity 102, thereby facilitating further improvement of the filtering speed of the filter member 30.

[0031] Optionally, in an embodiment of the present application, the plate member 10 is at least partially made of transparent material, and / or the sealing member 20 is made of transparent material. For example, when the detection principle of the detection agent is based on photosensitive reaction, the plate member 10 and / or the sealing member 20 can be configured as transparent material, so that the whole blood detection device 100 has better light transmittance, thereby ensuring the photosensitive reaction speed of the detection agent and improving the detection efficiency.

[0032] Optionally, in an embodiment of the present application, the filter member 30 is made of a filter membrane with a pore diameter of not greater than 1 μm, and the material of the filter membrane includes polycarbonate PC, water-based polyether sulfone PES, mixed cellulose MCE, and polytetrafluoroethylene PTFE. The filter member 30 can be in a membrane structure, for example, made of a PC membrane with a pore diameter of 0.8 μm. The blood cells in the whole blood sample can be separated and filtered and left in the collection cavity, which is convenient for subsequent detection, and the serum passes through the filter member 30 and enters the containing cavity 102 and is guided to the position of the detection agent by the flow assisting structure.

[0033] The whole blood detection device 100 of the embodiment does not need an external pump, and the negative pressure in the collection cavity and the flow guiding effect of the flow assisting structure can realize the pretreatment and filtration of whole blood and the one-time detection of multiple biochemical indexes.

[0034] In a second aspect, the embodiment of the present application provides a detection method of a whole blood detection device integrated with self-negative pressure and surface-modified flow assisting structure. When the whole blood detection device 100 is used, as shown in Figure 2As shown, the whole blood sample can be sucked by the needle tube 40, the needle pierces the sealing piece 20, and under the negative pressure of the collection cavity, the whole blood sample is quickly separated from the needle, and under the filtering effect of the filter piece 30, the object to be detected such as serum in the whole blood sample enters the containing cavity 102, and the impurities such as blood cells are filtered by the filter piece 30 and remain in the collection cavity; because the flow assisting structure arranged on the inner wall of the containing cavity 102 is subjected to hydrophilic treatment, the strong hydrophilic force of the flow assisting structure can quickly guide and suck the object to be detected to the position of the detection agent for detection reaction, and the operation process can ensure that the object to be detected in the whole blood sample flows accurately and quickly from the needle tip to the position of the detection agent, thereby forming a safe and complete whole blood detection process, and effectively preventing biological pollution.

[0035] The whole blood detection device 100 can be integrated in a structure size of 14mmx20mmx3.6mm, small in volume, convenient to carry, simple in operation, low in cost, and widely used in various application scenarios.

[0036] It should be noted that the relationship terms such as first and second in the present text are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitations, the element defined by the statement "including a" does not exclude the presence of other identical elements in the process, method, article or equipment including the element.

[0037] Each embodiment in the specification is described in a relevant manner, and the same and similar parts between each embodiment can be referred to each other, and each embodiment mainly explains the difference from other embodiments.

[0038] Especially, for the device embodiment, because it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the part of the method embodiment.

[0039] For the convenience of description, the above device is described as various units / modules respectively described in function. Of course, in the implementation of the present application, the functions of each unit / module can be realized in the same or multiple software and / or hardware.

[0040] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A whole blood testing device integrating a self-priming and surface-modified flow-aid structure, characterized in that, The utility model relates to a detection device for detecting object, which comprises: a plate provided with a containing cavity, one side of the plate further provided with a sample adding port, the sample adding port being communicated with the containing cavity, a detection agent being arranged in the containing cavity, and a flow assisting structure being further arranged in the containing cavity, the flow assisting structure being used for guiding the flow of the object to be detected; a sealing member for sealing the sample adding port; a filter arranged in the space enclosed by the sealing member and the plate, and a collection cavity being formed between the filter and the sealing member, the air pressure in the collection cavity being lower than the atmospheric pressure; the plate comprises an intermediate plate body, an upper plate body and a lower plate body, the intermediate plate body being located between the upper plate body and the lower plate body, a first adhesive layer being arranged between the intermediate plate body and the upper plate body, and a second adhesive layer being arranged between the intermediate plate body and the lower plate body, the sample adding port being arranged on the upper plate body; the sealing member is in the shape of a groove, the groove opening of the sealing member facing the plate, and the groove bottom of the sealing member facing away from the plate, the filter being arranged in the groove opening of the sealing member; the filter is in the shape of a groove, the groove opening of the filter facing the sealing member, and the groove bottom of the filter extending into the containing cavity; the detection agent is solidified at a preset position in the containing cavity, the containing cavity comprising a main cavity and at least one sub-cavity, the sub-cavity being communicated with the main cavity, and the detection agent being arranged in the sub-cavity; the flow assisting structure is arranged on the inner wall of the containing cavity, and the flow assisting structure has hydrophilicity.

2. The integrated self-priming and surface-modified flow-assist structure whole blood testing device of claim 1, wherein, the filter is made of a filter membrane with a pore diameter not greater than 1 μm.

Images