Extraction and separation device for glucoside in tobacco

Abstract

The device comprises an ultrasonic box, a macroporous resin separation column, an infusion pump and a container, the macroporous resin separation column is arranged in the ultrasonic box, extract liquor and eluent are separately contained in the container, the inlet end of the infusion pump is communicated with the container, and the outlet end of the infusion pump is communicated with the macroporous resin separation column; and the macroporous resin separation column is spiral. According to the extraction and separation device for the glucoside in the tobacco, the macroporous resin separation column is arranged in the ultrasonic box, and ultrasonic waves act on the macroporous resin separation column, so that the balance speed of macroporous resin during adsorption treatment of substances is increased, the flow rate of materials to be treated is increased, the treatment capacity of the resin per unit volume in unit time is improved, and the yield of the glucoside in the tobacco is increased. The adsorption and desorption efficiency of the macroporous resin is improved, and the technical problems of insufficient adsorption of the glucoside in the tobacco and high separation cost are solved.

Description

Technical Field

[0001] The present application relates to the technical field of extraction and separation of glycosides in tobacco, and in particular to a device for extraction and separation of glycosides in tobacco. Background Art

[0002] Glycosides in tobacco are non-volatile, latent aroma compounds that decompose during smoking to release aroma components. They are a crucial component of high-quality tobacco smoke. Glycosides are formed by the dehydration of the hemiacetal hydroxyl group of a cyclic sugar with the hydroxyl or amino group of another compound. These ligands can include terpenes, hydroxyesters, phenols, flavonoids, and other compounds, resulting in a wide variety of glycosides.

[0003] The current devices for separating glycosides from tobacco have disadvantages such as being unfavorable for removing organic solvents and being unfavorable for fully adsorbing glycosides. Utility Model Content

[0004] The utility model provides a device for extracting and separating glycosides in tobacco, so as to solve the technical problems of insufficient adsorption of glycosides in tobacco and high separation cost in the prior art.

[0005] In order to achieve the above-mentioned purpose, the technical solution provided by the present utility model is as follows:

[0006] The first aspect of the present utility model provides a device for extracting and separating glycosides in tobacco, comprising an ultrasonic box, a macroporous resin separation column, a liquid pump and a container, wherein the macroporous resin separation column is arranged in the ultrasonic box, the container is separately filled with an extract and an eluent, the inlet end of the liquid pump is connected to the container, and the outlet end is connected to the macroporous resin separation column; the macroporous resin separation column is spiral-shaped.

[0007] Furthermore, the macroporous resin separation column is clamped in the ultrasonic box.

[0008] Furthermore, the extraction and separation device further comprises a positioning post, which is fixed in the ultrasonic box and extends in a direction perpendicular to the bottom of the ultrasonic box, and the macroporous resin separation column is sleeved on the positioning post.

[0009] Furthermore, the device for extracting and separating glycosides in tobacco further comprises a first cover plate, wherein the first cover plate is arranged on the top of the ultrasonic box, and the container is fixed on the first cover plate.

[0010] Furthermore, a first through hole is formed on the first cover plate, and the outlet end of the liquid pump is connected to the inlet end of the macroporous resin separation column through the first through hole.

[0011] Furthermore, the container is divided into an extraction liquid chamber and a plurality of elution liquid chambers, and the inlet end of the liquid pump is switched to connect to the extraction liquid chamber or one of the elution liquid chambers.

[0012] Furthermore, the device for extracting and separating glycosides in tobacco further comprises a second cover plate, which is arranged on the top of the container.

[0013] Furthermore, a plurality of second through holes are formed on the second cover plate, and the extraction liquid chamber and the elution liquid chamber are respectively connected to the outside through a second through hole.

[0014] Furthermore, the second cover plate is movably connected to the top of the container.

[0015] Furthermore, a groove is formed on the top of the container, and the second cover moves in the groove to open or close the container.

[0016] The present invention provides a device for extracting and separating glycosides from tobacco. A macroporous resin separation column is placed within an ultrasonic chamber. Ultrasonic waves act on the macroporous resin separation column, accelerating the equilibrium speed of the macroporous resin during adsorption and treatment, increasing the flow rate of the material to be treated, and improving the processing capacity per unit volume of resin per unit time. This improves the adsorption and desorption efficiency of the macroporous resin, resolving the technical issues of insufficient adsorption of glycosides in tobacco and high separation costs. The macroporous resin separation column is configured in a spiral shape, achieving the maximum separation distance with the smallest volume, thereby improving separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments recorded in this application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 This is a schematic structural diagram of a device for extracting and separating glycosides from tobacco according to an embodiment of the present utility model.

[0019] Reference numerals:

[0020] 1. Ultrasonic chamber; 2. Macroporous resin separation column; 3. Liquid pump; 4. Container; 41. Extraction liquid chamber; 42. Eluent chamber; 43. Control panel; 5. First cover plate; 6. Second cover plate; 7. Observation hole. DETAILED DESCRIPTION

[0021] In order to help those skilled in the art better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making any creative efforts shall fall within the scope of protection of this application.

[0022] It should be noted that when an element is referred to as being “fixed on” or “set on” another element, it can be directly on the other element or indirectly set on the other element; when an element is referred to as being “connected to” another element, it can be directly connected to the other element or indirectly connected to the other element.

[0023] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0024] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout the description of this application, "plurality" or "several" means two or more, unless otherwise specifically defined.

[0025] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the conditions under which this application can be implemented. Therefore, they have no substantive technical significance. Any structural modification, change in proportional relationship or adjustment of size should still fall within the scope of the technical content disclosed in this application without affecting the efficacy and purpose that can be achieved by this application.

[0026] like Figure 1 As shown, an embodiment of the present application provides an extraction and separation device for glycosides in tobacco, comprising an ultrasonic box 1, a macroporous resin separation column 2, a liquid pump 3 and a container 4. The macroporous resin separation column 2 is arranged in the ultrasonic box 1, and the container 4 is separately filled with an extract and an eluent. The inlet end of the liquid pump 3 is connected to the container 4, and the outlet end is connected to the macroporous resin separation column 2; the macroporous resin separation column 2 is spiral.

[0027] In this embodiment, an ultrasonic chamber 1 is filled with water. When turned on, the ultrasonic effect is transmitted through the water to the macroporous resin separation column 2. As needed, a liquid extraction pump 3 can extract an extract or an eluent from a container 4 and pump the solution to the inlet of the macroporous resin separation column 2. The spiral shape of the macroporous resin separation column 2 increases the flow path of the solution, achieving the maximum separation distance with the minimum volume, thereby improving separation efficiency.

[0028] The apparatus for extracting and separating glycosides from tobacco in the embodiment of the present application comprises a macroporous resin separation column 2 disposed within an ultrasonic chamber 1. Ultrasonic waves act on the macroporous resin separation column 2, accelerating the equilibrium speed of the macroporous resin during adsorption of treated substances, increasing the flow rate of the material to be treated, and improving the processing capacity per unit volume of resin per unit time. This improves the adsorption and desorption efficiency of the macroporous resin, thereby resolving the technical issues of insufficient adsorption of glycosides in tobacco and high separation costs. The macroporous resin separation column 2 is configured in a spiral shape, enabling the maximum separation distance to be achieved with the minimum volume, thereby improving separation efficiency.

[0029] Furthermore, the macroporous resin separation column 2 is fixed in the ultrasonic box 1 to prevent the macroporous resin separation column 2 from moving, tilting or falling in the ultrasonic box 1 when the ultrasonic box 1 is opened.

[0030] Specifically, in some embodiments, the macroporous resin separation column 2 is snap-fitted into the ultrasonic chamber 1. By setting the inner diameter of the ultrasonic chamber 1 and the maximum outer diameter of the macroporous resin separation column 2, the macroporous resin separation column 2 can be directly snap-fitted into the ultrasonic chamber 1. Alternatively, a pop-up clip can be provided on the inner wall of the ultrasonic chamber 1. Once the macroporous resin separation column 2 is placed in the ultrasonic chamber 1, the clip pops out to snap into place. An observation hole 7 is provided on the outer wall of the ultrasonic chamber 1 to allow for observation of the macroporous resin separation column 2 at any time.

[0031] In other embodiments, the extraction and separation device further comprises a positioning post, which is fixed in the ultrasonic box 1 and extends in a direction perpendicular to the bottom of the ultrasonic box 1, and the macroporous resin separation column 2 is sleeved on the positioning post. Figure 1 A positioning column is installed in the ultrasonic box 1, and the bottom end of the positioning column is fixed to the bottom of the ultrasonic box 1. The connection method of the positioning column to the ultrasonic box 1 is not limited. It can be connected by screwing, which is convenient for disassembly; it can also be connected by snap-on connection. The positioning column extends in a direction perpendicular to the bottom of the ultrasonic box 1. The macroporous resin separation column 2 is sleeved on the positioning column so that the macroporous resin separation column 2 is fixed and does not move around under the action of ultrasonic waves. The extension direction of the positioning column is consistent with the direction of the central axis of the macroporous resin separation column 2. Furthermore, the central axis of the positioning column coincides with the central axis of the macroporous resin separation column 2.

[0032] In some embodiments, the device for extracting and separating glycosides in tobacco further includes a first cover plate 5, which is provided on the top of the ultrasonic box 1, and the container 4 is fixed on the first cover plate 5. In the embodiment of the present application, the first cover plate 5 is provided on the top of the ultrasonic box 1 and the container 4 is fixed on the above-mentioned first cover plate 5, so as to realize the integrated setting of the entire device and reduce the difficulty of personnel operation. The container 4 is fixedly connected to the first cover plate 5, which facilitates the installation of the container 4 on the ultrasonic box 1. Furthermore, a first through hole is formed on the first cover plate 5, and the outlet end of the liquid pump 3 is connected to the inlet end of the macroporous resin separation column 2 through the first through hole.

[0033] In some embodiments, the container 4 is divided into an extraction liquid chamber 41 and multiple elution liquid chambers 42, and the inlet end of the liquid pump 3 is switched to connect the extraction liquid chamber 41 or an elution liquid chamber 42. In the embodiment of the present application, the extraction liquid and multiple eluents are each contained in the separated chamber of the container 4, rather than being contained in multiple containers 4, which makes the overall transportation convenient. In the embodiment of the present application, the number of eluent chambers 42 is 3, and each eluent chamber 42 contains a different eluent, which is water, a mixture of ether and n-hexane in a volume ratio of 1:1, and methanol. The extract sample is added to the macroporous resin separation column 2, first eluted with water to remove sugars; then eluted with a mixture of ether and n-hexane to remove oils; and finally eluted with methanol to collect glycoside compounds.

[0034] Furthermore, the device for extracting and separating glycosides in tobacco further comprises a second cover plate 6, which is disposed on the top of the container 4. The second cover plate 6 is disposed on the top of the container 4 to prevent the liquid in the container 4 from being contaminated. A control panel 43 may be disposed on the outside of the container 4. The control panel 43 may display the amount of liquid in each compartment, i.e., the volume of the extract and the various eluents. The control panel 43 may also be used to control the opening and closing of the liquid pump 3, as well as the suction rate of the liquid pump 3.

[0035] In some embodiments, the second cover plate 6 is formed with a plurality of second through-holes, and the extraction liquid chamber 41 and the eluent liquid chamber 42 are each connected to the outside world through a second through-hole. It is understood that the second through-holes facilitate the addition of the corresponding liquid into the extraction liquid chamber 41 or the eluent liquid chamber 42 while the second cover plate 6 is fixed.

[0036] In other embodiments, the second cover 6 is movably connected to the top of the container 4. In the embodiments of the present application, the second cover 6 is preferably a movable cover. The container 4 can be opened by moving or removing the second cover 6. Specifically, a groove is formed in the top of the container 4, and the second cover 6 moves within the groove to open or close the container 4. The movable second cover 6 facilitates the full opening of the container 4, thereby adding the desired liquid to the container 4.

[0037] The tobacco glycoside extraction and separation device of the present embodiment utilizes the ultrasonic action of an ultrasonic chamber 1 to enhance the adsorption and desorption efficiency of tobacco glycosides by a macroporous resin separation column 2. Furthermore, the extraction chamber 41 and the elution chamber 42 are integrated into the same device, simplifying manual operation. A control panel 43 can be mounted on the outer wall of the container 4 or the ultrasonic chamber 1 to control the opening and closing of the liquid pump, simplifying operation.

[0038] When using the extraction and separation device of the embodiment of the present application to extract and separate glycosides in tobacco, the following steps are included:

[0039] (1) Methanol was added to the tobacco sample for ultrasonic extraction, the extract was filtered and concentrated to dryness, and then dissolved in deionized water to obtain an extract sample;

[0040] (2) Add the above extract sample into the extraction liquid chamber 41, and add eluent water, ether-n-hexane mixture, and methanol into the eluent chamber 42 respectively;

[0041] (3) Operate the control panel 43, start the liquid pump 3, and add the extract sample to the macroporous resin separation column 2 at a pumping speed of 50 mL / min; then, pump the eluent into the macroporous resin separation column 2 at a pumping speed of 50 mL / min. The contact between the solution and the macroporous resin can be checked through the observation hole 7 on the ultrasonic box 1, and the flow rate can be adjusted appropriately to collect the solution in the eluent chamber 42 containing methanol;

[0042] (4) The collected solution was evaporated to dryness, the volume was fixed, and the glycoside content in the solution was determined by liquid chromatography-mass spectrometry.

[0043] The above description of the disclosed embodiments will enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for extracting and separating glycosides from tobacco, characterized in that: The invention comprises an ultrasonic box, a macroporous resin separation column, a liquid extraction pump and a container. The macroporous resin separation column is arranged in the ultrasonic box. The extraction liquid and the eluent are separately contained in the container. The inlet end of the liquid extraction pump is connected to the container, and the outlet end is connected to the macroporous resin separation column. The macroporous resin separation column is spiral.

2. The device for extracting and separating glycosides in tobacco according to claim 1, characterized in that: The macroporous resin separation column is clamped in the ultrasonic box.

3. The device for extracting and separating glycosides in tobacco according to claim 1, characterized in that: The extraction and separation device further comprises a positioning post, which is fixed in the ultrasonic box and extends in a direction perpendicular to the bottom of the ultrasonic box. The macroporous resin separation column is sleeved on the positioning post.

4. The device for extracting and separating glycosides from tobacco according to any one of claims 1 to 3, characterized in that: The device for extracting and separating glycosides in tobacco further comprises a first cover plate, which is arranged on the top of the ultrasonic box, and the container is fixed on the first cover plate.

5. The device for extracting and separating glycosides in tobacco according to claim 4, characterized in that: A first through hole is formed on the first cover plate, and the outlet end of the liquid pump passes through the first through hole and is communicated with the inlet end of the macroporous resin separation column.

6. The device for extracting and separating glycosides in tobacco according to any one of claims 1 to 3, characterized in that: The container is divided into an extraction liquid chamber and a plurality of elution liquid chambers, and the inlet end of the liquid pump is switched to connect with the extraction liquid chamber or one of the elution liquid chambers.

7. The device for extracting and separating glycosides in tobacco according to claim 6, characterized in that: The device for extracting and separating glycosides in tobacco further comprises a second cover plate, which is arranged on the top of the container.

8. The device for extracting and separating glycosides in tobacco according to claim 7, characterized in that: A plurality of second through holes are formed on the second cover plate, and the extraction liquid chamber and the elution liquid chamber are respectively connected to the outside through a second through hole.

9. The device for extracting and separating glycosides in tobacco according to claim 7, characterized in that: The second cover plate is movably connected to the top of the container.

10. The device for extracting and separating glycosides in tobacco according to claim 9, characterized in that: A groove is formed on the top of the container, and the second cover moves in the groove to open or close the container.

Images