Crusher for microbial cell detection

Abstract

The utility model relates to the technical field of microbial cell detection, in particular to a crusher for microbial cell detection, which comprises a working table, a switching mechanism is arranged at the top of the working table, and a driving mechanism is arranged at the bottom of the working table and used for driving the switching mechanism to operate; through the arrangement of the switching mechanism, a plurality of magnetic suction containers for storing microbial cells to be crushed can be placed in a placement groove in a turntable, the turntable drives the magnetic suction containers to rotate so that the magnetic suction containers are crushed when passing through the lower part of an ultrasonic crushing rod every time, and then through the rotation of the turntable, the purpose of efficiently replacing samples for crushing is achieved; and when the crushed magnetic suction container moves to the position of the transfer magnetic block, the crushed magnetic suction container can be attracted by magnetism to slide out of the placement groove to the transfer magnetic block, and is moved into the magnetic suction groove in the tray to be transferred, so that the subsequent taking of workers is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial cell detection, in particular to a disruptor for microbial cell detection. Background Art

[0002] Microbial cell disruptors are commonly used in microbiological research and testing. Cell disruption technology involves the use of external forces to disrupt cell membranes and cell walls, releasing cellular contents, including the target product. It is commonly used to isolate and purify specific proteins, or to extract DNA and RNA. Cell disruption varies in difficulty and requires different methods for different organisms or tissues from different parts of the same organism. For example, the cell membranes of animal organs are fragile and easily broken, while plant and microbial cell walls, composed of cellulose and hemicellulose, are more difficult to disrupt. Generally, during the DNA extraction process, laboratories manually grind the microbial cells in a mortar. However, manual disruption is time-consuming and labor-intensive, and prone to tipping, creating significant inconvenience for experimental research. Some researchers have used existing cell disruptors, but most utilize fixed machines for cell disruption and then use external instruments to remove the disrupted cells. This process not only wastes a large number of cells but is also very time-consuming.

[0003] Prior art, such as publication number CN214991557U, provides a microbial cell disruptor comprising a disruption chamber comprising a base and a disruption chamber. The disruption chamber houses a cell disruption component comprising an ultrasonic vibrator and a cell disruption tank rotatably connected to the base. The cell disruption tank houses a cell transfer sleeve rotatably connected to the cell disruption tank. The present invention utilizes the cell disruption component to disrupt microbial cells, while the cell transfer sleeve is used for transferring the microbial cells, saving significant time and labor.

[0004] In this solution, microbial cells are crushed by setting a cell crushing device. The cell transfer sleeve set in the cell crushing tank is removable. After the microbial cells are crushed, the cell transfer sleeve can be directly taken out and poured into the required container. However, it still requires manual labor to take them out. When large-scale crushing operations are required, the cell transfer sleeve is connected by a threaded rod and a threaded hole. As a result, the disassembly and transfer process consumes a lot of time during batch operations and places a heavy burden on the operator. In view of this, we propose a microbial cell crusher for detection. Utility Model Content

[0005] The purpose of the present utility model is to provide a microbial cell disruptor for detecting microorganisms, which solves the problem in the comparative technology that a cell transfer sleeve is connected by a threaded rod and a threaded hole, resulting in a large amount of time being consumed in the disassembly and transfer process during batch operations.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] A microbial cell disruptor comprises a workbench, a switching mechanism is provided on the top of the workbench, and a driving mechanism is provided on the bottom of the workbench for driving the switching mechanism to operate;

[0008] The switching mechanism includes a turntable, which is rotatably connected to the left side of the workbench, and a tray is rotatably connected to the right side of the workbench. A transfer magnetic block is connected to the top of the workbench between the tray and the turntable. A placement slot is provided on the top of the turntable, and the placement slot is used to place magnetic containers. There are six placement slots, which are distributed in a circular array. Multiple magnetic slots are provided on the top of the tray, and the magnetic slots are used to adsorb magnetic containers. The positions of the magnetic slots correspond one-to-one to the positions of the placement slots.

[0009] Preferably, the tray is connected to a squeezing block at a position outside each magnetic slot for pushing the magnetic container to slide inside.

[0010] Preferably, a stopper is connected to the top of the workbench at a position on the back of the transfer magnetic block, and a notch is provided at the bottom of each extrusion block at a position corresponding to the stopper.

[0011] Preferably, a support frame is connected to the left side of the top of the workbench, a cylinder is connected below the top of the support frame, an ultrasonic breaker rod is connected to the bottom of the cylinder, and the position of the ultrasonic breaker rod corresponds to the magnetic container in the placement slot.

[0012] Preferably, the driving mechanism includes a gear, which is rotatably connected to the bottom of the workbench, and the gear is connected to the bottom of the turntable. The bottom of the workbench is rotatably connected to a gear disk, and the gear disk is connected to the bottom of the turntable. The outer wall of the gear disk is connected to teeth, and the teeth are meshed with the gear. The bottom of the workbench is connected to a stepper motor through a mounting bracket, and the output end shaft of the stepper motor is connected to the gear disk.

[0013] Preferably, three groups of teeth are distributed on the outer wall of the toothed disc, which are arranged in a circular array, and the distribution range of each group of teeth is one-sixth of the circle of the toothed disc.

[0014] Preferably, a lifting assembly is provided at the position of the tray on the workbench, and the lifting assembly includes a bracket, and the bracket is arranged on the path of rotation and movement of the magnetic suction slot. The top of the workbench is connected to a bracket, and the top of the bracket is connected to an arc guide rail, and the bracket is slidably connected to the arc guide rail through a slider.

[0015] By means of the above technical solution, the present invention provides a microbial cell disruptor for detection, which has at least the following beneficial effects:

[0016] 1. The utility model is provided with a switching mechanism. A plurality of magnetic containers for storing microbial cells to be crushed can be placed in the slots on the turntable. The magnetic containers are driven by the turntable to rotate so that each time they pass under the ultrasonic crushing rod, they are crushed. Then, by the rotation of the turntable, the purpose of efficiently changing samples for crushing is achieved, which greatly improves the efficiency in batch operations. When the crushed magnetic container moves to the position of the transfer magnetic block, it will be magnetically attracted to slide out of the placement slot and onto the transfer magnetic block, and then moved to the magnetic slot on the tray for transportation, which is convenient for subsequent staff to take.

[0017] 2. The utility model is provided with a lifting component. Each time the magnetic suction groove is rotated to the position of the bracket, the bracket is just stuck under the support ring of the magnetic container in the magnetic suction groove. As the tray rotates subsequently, the magnetic suction groove pushes the magnetic container in the bracket to be gradually lifted up, breaking away from the magnetic attraction of the magnetic suction groove, so as to facilitate subsequent staff to take it. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application:

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 This is a structural diagram showing the driving mechanism of the present utility model;

[0021] Figure 3 This is a structural diagram showing the switching mechanism in the present utility model;

[0022] Figure 4 This is a structural diagram showing the lifting component in the present invention.

[0023] In the figure: 1. workbench; 2. support frame; 3. cylinder; 4. ultrasonic breaker rod; 5. switching mechanism; 50. magnetic container; 51. turntable; 52. placement slot; 53. tray; 54. transfer magnet; 55. magnetic slot; 551. extrusion block; 56. stop block; 561. notch; 6. lifting part; 61. bracket; 62. arc guide rail; 63. slider; 64. bracket; 7. driving mechanism; 71. gear; 72. gear disc; 721. teeth; 73. stepping motor; 74. mounting frame. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1

[0026] A microbial cell disruptor, such as Figure 1 、 Figure 3 As shown, it includes a workbench 1, a switching mechanism 5 is provided on the top of the workbench 1, and a driving mechanism 7 is provided at the bottom of the workbench 1 for driving the switching mechanism 5 to operate. The switching mechanism 5 includes a turntable 51, the turntable 51 is rotatably connected to the left side of the workbench 1, and the right side of the workbench 1 is rotatably connected to the tray 53. The top of the workbench 1 is connected to a transfer magnetic block 54 between the tray 53 and the turntable 51. The top of the turntable 51 is provided with a placement slot 52, and the placement slot 52 is used to place the magnetic container 50. There are six placement slots 52, which are distributed in a circular array. The top of the tray 53 is provided with multiple magnetic slots 55, and the magnetic slots 55 are used to adsorb the magnetic container 50. The position of the magnetic slot 55 corresponds to the position of the placement slot 52. The left side of the top of the workbench 1 is connected to a support frame 2, and the bottom of the support frame 2 is connected to an air cylinder 3. The bottom of the cylinder 3 is connected to an ultrasonic breaker rod 4, and the position of the ultrasonic breaker rod 4 corresponds to the magnetic container 50 in the placement slot 52.

[0027] In this embodiment, by providing a switching mechanism 5, a plurality of magnetic containers 50 for storing microbial cells to be crushed can be placed in the slots 52 on the turntable 51. The magnetic container 50 is driven to rotate by the turntable 51 so that each time it passes under the ultrasonic crushing rod 4, the cylinder 3 drives the ultrasonic crushing rod 4 to be inserted into the magnetic container 50. By generating high-frequency vibration in the liquid, an ultrasonic field is formed. When the microbial cells are in the ultrasonic field, the cells are affected by various effects such as cavitation, mechanical vibration and microjet of the ultrasonic wave, thereby causing the cells to be crushed, thereby achieving the effect of crushing the cells. When the ultrasonic crushing rod 4 is lifted and switched to the next magnetic container 50 for crushing, the rotation of the turntable 51 achieves the purpose of efficiently changing the sample for crushing, which greatly improves the efficiency in batch operation. When the crushed magnetic container 50 moves to the position of the transfer magnetic block 54, it will be magnetically attracted to slide out of the placement slot 52 onto the transfer magnetic block 54, and then moved to the magnetic slot 55 on the tray 53 for transportation, which is convenient for subsequent staff to take.

[0028] Example 2

[0029] like Figure 3 As shown, the tray 53 is connected to an extrusion block 551 at the outside of each magnetic slot 55, which is used to push the magnetic container 50 to slide in. The top of the workbench 1 is connected to a stop block 56 at the back of the transfer magnetic block 54, and a notch 561 is provided at the bottom of each extrusion block 551 corresponding to the position of the stop block 56.

[0030] In this embodiment, an extrusion block 551 is provided to cooperate with the stop block 56 , and the extrusion force between the extrusion block 551 and the stop block 56 is generated when the tray 53 rotates, so as to facilitate the magnetic container 50 that slides to the outside of the magnetic groove 55 to be completely squeezed into the magnetic groove 55 .

[0031] Example 3

[0032] like Figure 1 、 Figure 2 As shown, the driving mechanism 7 includes a gear 71, which is rotatably connected to the bottom of the workbench 1, and the gear 71 is connected to the bottom of the turntable 51. The bottom of the workbench 1 is rotatably connected to a toothed disc 72, and the toothed disc 72 is connected to the bottom of the turntable 51. The outer wall of the toothed disc 72 is connected to teeth 721, and the teeth 721 are meshed with the gear 71. The bottom of the workbench 1 is connected to a stepper motor 73 through a mounting bracket 74, and the output end shaft of the stepper motor 73 is connected to the toothed disc 72. Three groups of teeth 721 are distributed on the outer wall of the toothed disc 72, which are distributed in a circular array, and the distribution range of each group of teeth 721 is one-sixth of a circle of the toothed disc 72.

[0033] In this embodiment, the stepper motor 73 is started to drive the gear disc 72 to perform an intermittent rotation of sixty degrees, and the one-sixth circle of teeth 721 set on the outside of the gear disc 72 drives the engaged turntable 51 to rotate, so that the gear disc 72 drives the turntable 51 to rotate sixty degrees every one hundred and twenty degrees.

[0034] Example 4

[0035] like Figure 4 、 Figure 4 As shown, a lifting assembly 6 is provided at the position of the tray 53 on the workbench 1. The lifting assembly 6 includes a bracket 64. The bracket 64 is arranged on the path of rotation and movement of the magnetic suction groove 55. The top of the workbench 1 is connected to a bracket 61. The top of the bracket 61 is connected to an arc guide rail 62. The bracket 64 is slidably connected to the arc guide rail 62 through a slider 63.

[0036] In this embodiment, by setting up a lifting component 6, each time the magnetic slot 55 rotates to the position of the bracket 64, the bracket 64 is just stuck under the support ring of the magnetic container 50 in the magnetic slot 55. As the tray 53 rotates subsequently, the magnetic container 50 in the bracket 64 will be gradually lifted up under the push of the magnetic slot 55, and will be separated from the magnetic attraction of the magnetic slot 55, so as to facilitate subsequent staff to take it.

[0037] When in use, a microbial cell detection crusher of the present invention can be configured to place a plurality of magnetic containers 50 for storing microbial cells to be crushed in the slots 52 placed on the turntable 51, and start the stepping motor 73 to drive the gear disc 72 to perform an intermittent rotation of sixty degrees. The gear teeth 721 of one-sixth circle provided on the outside of the gear disc 72 drive the meshing turntable 51 to rotate, so that the gear disc 72 drives the turntable 51 to rotate sixty degrees every time it rotates one hundred and twenty degrees. The magnetic container 50 is driven to rotate by the turntable 51 so that each time it passes under the ultrasonic crushing rod 4, the ultrasonic crushing rod 4 is driven by the cylinder 3 to be inserted into the magnetic container 50, and an ultrasonic field is formed by generating high-frequency vibration in the liquid. When the microbial cells are in the ultrasonic field, the cells are affected by various effects such as ultrasonic cavitation, mechanical vibration and microjet, thereby causing the cells to be crushed, so as to achieve the effect of crushing the cells. When the ultrasonic crushing rod 4 is lifted by the cylinder 3 to switch to the next magnetic container 50, the ultrasonic crushing rod 4 is driven by the cylinder 3 to be inserted into the magnetic container 50. The crushing is carried out by rotating the turntable 51, so as to achieve the purpose of efficiently changing the sample for crushing, which greatly improves the efficiency in batch operation. When the crushed magnetic container 50 moves to the position of the transfer magnetic block 54, it will be magnetically attracted to slide out of the placement slot 52 and onto the transfer magnetic block 54, and then move to the magnetic slot 55 on the tray 53 for transportation. The extrusion block 551 is provided to cooperate with the stop block 56, and the extrusion force between the extrusion block 551 and the stop block 56 is used when the tray 53 rotates to facilitate The magnetic container 50 that has slid to the outside of the magnetic slot 55 is completely squeezed into the magnetic slot 55 to ensure that the magnetic container 50 can enter smoothly, and each time the magnetic slot 55 is rotated to the position of the bracket 64, the bracket 64 is just stuck under the support ring of the magnetic container 50 in the magnetic slot 55. As the tray 53 rotates subsequently, the magnetic container 50 in the bracket 64 will be gradually lifted up under the push of the magnetic slot 55, and will be freed from the magnetic attraction of the magnetic slot 55, so as to facilitate subsequent staff to take it.

[0038] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microbial cell disruptor for detecting microorganisms, comprising a workbench (1), characterized in that: A switching mechanism (5) is provided on the top of the workbench (1), and a driving mechanism (7) is provided on the bottom of the workbench (1) for driving the switching mechanism (5) to operate; The switching mechanism (5) comprises a turntable (51), the turntable (51) being rotatably connected to the left side of the workbench (1), the right side of the workbench (1) being rotatably connected to a tray (53), the top of the workbench (1) being connected to a switching magnetic block (54) between the tray (53) and the turntable (51), the top of the turntable (51) being provided with a placement slot (52), the placement slot (52) being used to place a magnetic container (50), the number of the placement slots (52) being six and being distributed in a circular array, the top of the tray (53) being provided with a plurality of magnetic slots (55), the magnetic slots (55) being used to adsorb the magnetic container (50), the positions of the magnetic slots (55) corresponding one to one to the positions of the placement slots (52).

2. A microbial cell disruptor for detection according to claim 1, characterized in that: The tray (53) is connected to a squeeze block (551) at a position outside each magnetic suction groove (55) for pushing the magnetic suction container (50) to slide inside.

3. A microbial cell disruptor for detection according to claim 2, characterized in that: A stopper (56) is connected to the top of the workbench (1) at a position on the back of the transfer magnetic block (54), and a notch (561) is provided at the bottom of each extrusion block (551) at a position corresponding to the stopper (56).

4. The microbial cell disruptor according to claim 1, wherein: A support frame (2) is connected to the left side of the top of the workbench (1), a cylinder (3) is connected below the top of the support frame (2), an ultrasonic breaker rod (4) is connected to the bottom of the cylinder (3), and the position of the ultrasonic breaker rod (4) corresponds to the magnetic container (50) in the placement groove (52).

5. The microbial cell disruptor according to claim 1, characterized in that: The driving mechanism (7) includes a gear (71), the gear (71) is rotatably connected to the bottom of the workbench (1), and the gear (71) is connected to the bottom of the turntable (51). The bottom of the workbench (1) is rotatably connected to a toothed disc (72), and the toothed disc (72) is connected to the bottom of the turntable (51). The outer wall of the toothed disc (72) is connected to teeth (721), and the teeth (721) are meshed with the gear (71). The bottom of the workbench (1) is connected to a stepping motor (73) through a mounting frame (74), and the output end shaft of the stepping motor (73) is connected to the toothed disc (72).

6. The microbial cell disruptor according to claim 5, characterized in that: Three groups of teeth (721) are distributed on the outer wall of the toothed disc (72), which are arranged in a circular array, and the distribution range of each group of teeth (721) is one-sixth of the circle of the toothed disc (72).

7. The microbial cell disruptor according to claim 3, characterized in that: The workbench (1) is provided with a lifting assembly (6) at the position of the tray (53), and the lifting assembly (6) includes a bracket (64). The bracket (64) is arranged on the path of rotation and movement of the magnetic suction groove (55). The top of the workbench (1) is connected to a bracket (61), and the top of the bracket (61) is connected to an arc guide rail (62). The bracket (64) is slidably connected to the arc guide rail (62) through a slider (63).

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