Culture equipment for promoting secretion of cell exosomes

Abstract

The utility model relates to the technical field of bioengineering, and provides a culture device for promoting cell exosome secretion, which comprises a base, the top of the base is fixedly connected with a thermotank, and the top of the inner cavity of the thermotank is fixedly connected with a liquid collecting box. By arranging the quantitative holes, the valve, the driving motor, the rotating shaft and the round blocks, when the driving motor operates, the rotating shaft can drive the rotating block to rotate, then the two round blocks rotate to relieve the sealing effect on the two quantitative holes, and at the moment, the two quantitative holes are filled with nutrient solution in the liquid collecting box; then the two round blocks are reset to close the two quantitative holes, at the moment, the valve is opened, and the nutrient solution in the two quantitative holes is discharged through the two liquid discharging pipes, so that the purpose of quantitatively adding the nutrient solution is achieved, the problem that errors exist in nutrient solution adding is solved, and the accuracy of nutrient solution proportional adding is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, and in particular to a culture device for promoting the secretion of extracellular vesicles. Background Art

[0002] Exosomes are tiny vesicles secreted by cells, with a diameter usually between 30-150 nanometers. Exosomes play an important role in intercellular communication. They can be taken up by neighboring cells or distant cells and deliver the bioactive molecules they carry to recipient cells, thereby regulating the physiological functions of the recipient cells.

[0003] In order to promote the secretion of extracellular vesicles, culture equipment is often used. In actual use, although the existing culture equipment can achieve the function of culturing cells, most of the existing culture devices use a syringe inserted into a rubber tube when adding nutrient solution, which prevents external bacteria from entering the culture bottle. A portion of the nutrient solution will remain when flowing in the rubber tube, resulting in an error in the proportion of nutrient solution added, thereby affecting the growth and secretion of extracellular vesicles. Therefore, it needs to be improved. Utility Model Content

[0004] The utility model provides a culture device for promoting the secretion of extracellular vesicles, which solves the problem of error in the addition ratio of nutrient solution in the related art.

[0005] The technical solution of the utility model is as follows: a culture device for promoting the secretion of cell exosomes, comprising a base, the top of the base is fixedly connected to a constant temperature box, the top of the inner cavity of the constant temperature box is fixedly connected to a liquid collecting box, a quantitative hole is opened inside the bottom of the liquid collecting box, a valve located directly below the quantitative hole is fixedly installed on the bottom of the liquid collecting box, a drain pipe is fixedly connected below the valve, a protective shell is fixedly installed in the middle of the top of the constant temperature box, a driving motor is fixedly installed inside the protective shell, the other end of the output shaft of the driving motor is fixedly sleeved with a rotating shaft, the bottom of the rotating shaft passes through the protective shell and the constant temperature box in sequence and extends to the interior of the constant temperature box and is fixedly sleeved with a rotating block, the bottom of the rotating block is movably connected to the bottom of the inner cavity of the liquid collecting box, and the left and right ends of the rotating block are fixedly connected to round blocks, the number of the round blocks is two, and the bottoms of the two round blocks are movably connected to the bottom of the inner cavity of the liquid collecting box.

[0006] As a preferred technical solution of the present invention, the top of the constant temperature box is fixedly connected to a liquid inlet pipe located on the left side of the protective shell, and the top of the liquid inlet pipe is wrapped with a rubber leather cover.

[0007] As an optimal technical solution of the present invention, heating plates are fixedly installed on the inner walls on both sides of the constant temperature box. There are two heating plates, the two heating plates have the same size, and the two heating plates are symmetrical with respect to the center of the constant temperature box.

[0008] As a preferred technical solution of the present invention, a rectangular door is hinged on the front of the constant temperature box, and a handle is fixedly connected to the left side of the front of the rectangular door.

[0009] As a preferred technical solution of the present invention, a glass window is fixedly installed inside the front surface of the rectangular door, and the outer surface of the glass window is smooth.

[0010] As an optimal technical solution of the present invention, a transmission motor is fixedly connected to the bottom of the base inner cavity, the other end of the transmission motor output shaft is fixedly sleeved with a transmission shaft, and the outer surface of the transmission shaft is fixedly sleeved with a first gear.

[0011] As a preferred technical solution of the present invention, the outer surface of the first gear is meshedly connected to the second gear, and the number of the second gears is two. A circular shaft is fixedly sleeved inside the two second gears, and a circular disc is fixedly sleeved on the outer surface of the top end of the circular shaft, and the outer surface of the circular disc is movably connected to the inner surface of the bottom of the constant temperature box.

[0012] As a preferred technical solution of the present invention, a culture bottle is movably connected to the top of the disc, and the outer surface of the culture bottle is movably connected to the inner surface of the bottom of the constant temperature box.

[0013] As a preferred technical solution of the present invention, the top of the disc is fixedly connected to a limiting block, and the outer surface of the limiting block is movably connected to the inner surface of the bottom of the culture bottle.

[0014] As a preferred technical solution of the present invention, there are two culture bottles, and the diameters of the bottoms of the two culture bottles are the same as the inner diameter of the bottom of the constant temperature box.

[0015] The beneficial effects of the utility model are:

[0016] 1. The utility model is provided with a quantitative hole, a valve, a driving motor, a rotating shaft and a round block. When the driving motor is running, the rotating shaft will drive the rotating block to rotate, and then the two round blocks will rotate to release the sealing effect of the two quantitative holes. At this time, the nutrient solution inside the liquid collection box will fill the two quantitative holes, and then the two round blocks will reset to seal the two quantitative holes. At this time, opening the valve will cause the nutrient solution inside the two quantitative holes to be discharged through the two drainage pipes, thereby achieving the purpose of quantitative addition of nutrient solution, solving the problem of error in nutrient solution addition, and improving the accuracy of nutrient solution proportional addition.

[0017] 2. The utility model is provided with a transmission motor, a transmission shaft, a first gear, a second gear and a disc. When the transmission motor is running, the transmission shaft will drive the first gear to rotate, and then the first gear will mesh with the two second gears and drive the two discs to rotate. Under the limiting action of the limiting block, the two culture bottles are driven to rotate, thereby achieving the purpose of accelerating the rapid and uniform dispersion of the nutrient solution into the culture bottle, solving the problem of uneven dispersion of the nutrient solution, and promoting the growth and secretion of cell exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0019] Figure 1 This is a schematic diagram of the structure of the utility model;

[0020] Figure 2 This is a schematic diagram of the cross-sectional structure of the utility model;

[0021] Figure 3 This is a schematic cross-sectional view of the side structure of the present invention;

[0022] Figure 4 This is a schematic cross-sectional view of the liquid collecting box of the present invention;

[0023] Figure 5 This is a schematic cross-sectional structural diagram of the first gear of the present invention.

[0024] In the figure: 1. Base; 2. Constant temperature box; 3. Liquid collecting box; 4. Quantitative hole; 5. Valve; 6. Drain pipe; 7. Protective shell; 8. Drive motor; 9. Rotating shaft; 10. Rotating block; 11. Round block; 12. Liquid inlet pipe; 13. Rubber cover; 14. Heating plate; 15. Rectangular door; 16. Handle; 17. Glass window; 18. Drive motor; 19. Drive shaft; 20. First gear; 21. Second gear; 22. Round shaft; 23. Disc; 24. Culture bottle; 25. Limit block. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] like Figures 1 to 5As shown, the utility model provides a culture device for promoting the secretion of extracellular vesicles, including a base 1, a thermostat 2 is fixedly connected to the top of the base 1, a liquid collecting box 3 is fixedly connected to the top of the inner cavity of the thermostat 2, a quantitative hole 4 is opened inside the bottom of the liquid collecting box 3, a valve 5 located directly below the quantitative hole 4 is fixedly installed at the bottom of the liquid collecting box 3, a drain pipe 6 is fixedly connected to the bottom of the valve 5, a protective shell 7 is fixedly connected to the middle part of the top of the thermostat 2, a driving motor 8 is fixedly installed inside the protective shell 7, the other end of the output shaft of the driving motor 8 is fixedly sleeved with a rotating shaft 9, the bottom of the rotating shaft 9 passes through the protective shell 7 and the thermostat 2 in sequence and extends to the interior of the thermostat 2 and is fixedly sleeved with a rotating block 10, the bottom of the rotating block 10 is movably connected to the bottom of the inner cavity of the liquid collecting box 3, and the left and right ends of the rotating block 10 are fixedly connected to round blocks 11, and there are two round blocks 11, and the bottoms of the two round blocks 11 are both movably connected to the bottom of the inner cavity of the liquid collecting box 3.

[0027] When the driving motor 8 is running, the rotating shaft 9 will drive the rotating block 10 to rotate, and then the two round blocks 11 will rotate to release the sealing effect of the two quantitative holes 4. At this time, the nutrient solution inside the liquid collection box 3 will fill the two quantitative holes 4, and then the two round blocks 11 will reset to seal the two quantitative holes 4. At this time, opening the valve 5 will allow the nutrient solution inside the two quantitative holes 4 to be discharged through the two drainage pipes 6, thereby achieving the purpose of quantitative addition of nutrient solution.

[0028] The top of the thermostatic box 2 is fixedly connected to a liquid inlet pipe 12 located on the left side of the protective shell 7 , and the top of the liquid inlet pipe 12 is wrapped with a rubber cover 13 .

[0029] The design of the rubber sheath 13 facilitates the operator to insert the syringe into the rubber sheath 13 , while preventing external bacteria from entering the interior of the liquid inlet tube 12 .

[0030] Among them, heating plates 14 are fixedly installed on the inner walls of the left and right sides of the constant temperature box 2. There are two heating plates 14, the two heating plates 14 have the same size, and the two heating plates 14 are symmetrical with each other about the center of the constant temperature box 2.

[0031] The design of the heating plate 14 facilitates heating the interior of the constant temperature box 2 .

[0032] A rectangular door 15 is hinged on the front of the thermostat box 2 , and a handle 16 is fixedly connected to the left side of the front of the rectangular door 15 .

[0033] By pulling the handle 16 , the rectangular door 15 can be driven to rotate and open, thereby releasing the sealing effect on the thermostat box 2 .

[0034] A glass window 17 is fixedly installed inside the front of the rectangular door 15, and the outer surface of the glass window 17 is smooth.

[0035] The design of the glass window 17 makes it easy for the operator to observe the secretion of extracellular vesicles inside the incubator 2 through the glass window 17 .

[0036] The bottom of the inner cavity of the base 1 is fixedly connected with a transmission motor 18 , the other end of the output shaft of the transmission motor 18 is fixedly sleeved with a transmission shaft 19 , and the outer surface of the transmission shaft 19 is fixedly sleeved with a first gear 20 .

[0037] When the transmission motor 18 is running, the transmission shaft 19 will drive the first gear 20 to rotate.

[0038] Among them, the outer surface of the first gear 20 is meshedly connected with the second gear 21, and the number of the second gears 21 is two. The inside of the two second gears 21 is fixedly sleeved with a circular shaft 22, and the outer surface of the top of the circular shaft 22 is fixedly sleeved with a disc 23, and the outer surface of the disc 23 is movably connected to the inner surface of the bottom of the constant temperature box 2.

[0039] The first gear 20 rotates and meshes with the two second gears 21 and drives the two disks 23 to rotate.

[0040] The top of the disc 23 is movably connected to a culture bottle 24 , and the outer surface of the culture bottle 24 is movably connected to the inner surface of the bottom of the constant temperature box 2 .

[0041] The design of the culture flask 24 is convenient for culturing cell exosomes.

[0042] The top of the disc 23 is fixedly connected to a limiting block 25 , and the outer surface of the limiting block 25 is movably connected to the inner surface of the bottom of the culture bottle 24 .

[0043] The design of the limiting block 25 serves to limit the position of the culture bottle 24 .

[0044] There are two culture bottles 24 , and the diameters of the bottoms of the two culture bottles 24 are the same as the inner diameter of the bottom of the constant temperature box 2 .

[0045] Such a design facilitates the culture bottle 24 to be placed better inside the constant temperature box 2 without shaking.

[0046] The working principle and use process of this utility model:

[0047] In order to promote the secretion of extracellular exosomes, it is necessary to quantitatively add nutrient solution to the exosomes. First, the drive motor 8 is started, which will cause the rotating shaft 9 to drive the rotating block 10 to rotate, and then the two round blocks 11 are rotated to release the sealing effect of the two quantitative holes 4. At this time, the nutrient solution inside the liquid collection box 3 will fill the two quantitative holes 4, and then the two round blocks 11 are reset to seal the two quantitative holes 4. At this time, the valve 5 is opened, and the nutrient solution inside the two quantitative holes 4 will be discharged through the two drainage pipes 6, thereby achieving the purpose of quantitative addition of nutrient solution.

[0048] When the nutrient solution is added to the interior of the culture bottle 24, the transmission motor 18 is started at this time, which will cause the transmission shaft 19 to drive the first gear 20 to rotate, and then the first gear 20 is engaged with the two second gears 21 and drives the two discs 23 to rotate. Under the limiting action of the limit block 25, the two culture bottles 24 are driven to rotate, so that the nutrient solution quickly and evenly fuses with the cell exosomes, thereby promoting the secretion of cell exosomes.

[0049] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A culture device for promoting the secretion of extracellular vesicles, comprising a base (1), characterized in that: The top of the base (1) is fixedly connected to a thermostat (2), the top of the inner cavity of the thermostat (2) is fixedly connected to a liquid collecting box (3), a quantitative hole (4) is provided inside the bottom of the liquid collecting box (3), a valve (5) located directly below the quantitative hole (4) is fixedly installed at the bottom of the liquid collecting box (3), a drain pipe (6) is fixedly connected below the valve (5), a protective shell (7) is fixedly connected to the middle of the top of the thermostat (2), a driving motor (8) is fixedly installed inside the protective shell (7), and the driving motor (8) is fixedly installed inside the protective shell (7). The other end of the output shaft of the machine (8) is fixedly sleeved with a rotating shaft (9), the bottom of the rotating shaft (9) sequentially passes through the protective shell (7) and the constant temperature box (2) and extends to the interior of the constant temperature box (2) and is fixedly sleeved with a rotating block (10), the bottom of the rotating block (10) is movably connected to the bottom of the inner cavity of the liquid collecting box (3), and the left and right ends of the rotating block (10) are fixedly connected with round blocks (11), the number of the round blocks (11) is two, and the bottoms of the two round blocks (11) are movably connected to the bottom of the inner cavity of the liquid collecting box (3).

2. The culture device for promoting exosome secretion according to claim 1, characterized in that: The top of the thermostatic box (2) is fixedly connected to a liquid inlet pipe (12) located on the left side of the protective shell (7), and the top of the liquid inlet pipe (12) is wrapped with a rubber sheath (13).

3. The culture device for promoting exosome secretion according to claim 1, characterized in that: Heating plates (14) are fixedly mounted on the inner walls of the left and right sides of the constant temperature box (2). There are two heating plates (14), the two heating plates (14) have the same size, and the two heating plates (14) are symmetrical with respect to the center of the constant temperature box (2).

4. The culture device for promoting exosome secretion according to claim 1, characterized in that: A rectangular door (15) is hinged on the front of the constant temperature box (2), and a handle (16) is fixedly connected to the left side of the front of the rectangular door (15).

5. The culture device for promoting exosome secretion according to claim 4, characterized in that: A glass window (17) is fixedly installed inside the front surface of the rectangular door (15), and the outer surface of the glass window (17) is smooth.

6. The culture device for promoting exosome secretion according to claim 1, characterized in that: A transmission motor (18) is fixedly connected to the bottom of the inner cavity of the base (1), a transmission shaft (19) is fixedly sleeved on the other end of the output shaft of the transmission motor (18), and a first gear (20) is fixedly sleeved on the outer surface of the transmission shaft (19).

7. The culture device for promoting exosome secretion according to claim 6, characterized in that: The outer surface of the first gear (20) is meshedly connected with a second gear (21), and the number of the second gears (21) is two. A circular shaft (22) is fixedly sleeved inside each of the two second gears (21), and a circular disk (23) is fixedly sleeved on the outer surface of the top end of the circular shaft (22), and the outer surface of the circular disk (23) is movably connected to the inner surface of the bottom of the constant temperature box (2).

8. The culture device for promoting exosome secretion according to claim 7, characterized in that: The top of the disc (23) is movably connected to a culture bottle (24), and the outer surface of the culture bottle (24) is movably connected to the inner surface of the bottom of the constant temperature box (2).

9. The culture device for promoting exosome secretion according to claim 7, characterized in that: The top of the disc (23) is fixedly connected to a limiting block (25), and the outer surface of the limiting block (25) is movably connected to the inner surface of the bottom of the culture bottle (24).

10. The culture device for promoting exosome secretion according to claim 8, characterized in that: There are two culture bottles (24), and the diameters of the bottoms of the two culture bottles (24) are the same as the inner diameter of the bottom of the constant temperature box (2).

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