Pretreatment purification column for detection of various fungaltoxins

Abstract

The utility model belongs to the field of mycotoxin detection, particularly relates to a pretreatment purification column for detection of various mycotoxins, and aims to solve the problems that an existing purification column can only treat one sample liquid at a time, and when a large amount of sample liquid needs to be treated, much time and energy need to be consumed, and inconvenience is caused. A fixing plate is fixedly arranged between the inner walls of the two sides of the frame body, a plurality of through holes are formed in the surface of the fixing plate in a penetrating mode, needle cylinders are arranged in the through holes, and adsorption filler is arranged on the inner sides of the needle cylinders; a motor and a controller are fixedly arranged on the surface of the frame body, one end of an output shaft of the motor penetrates through the frame body and is fixedly provided with a screw rod, multiple sample solutions can be treated at a time, manual pushing is not needed, the time and energy of experimenters are saved, the connecting rod and the needle cylinder are disposable articles, and the operation is convenient. After being used, the device can be conveniently taken down and discarded by experimenters, and harm and cross contamination of toxins to human bodies in the repeated using and cleaning process are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of mycotoxin detection, in particular to a pre-treatment purification column for detecting multiple mycotoxins. Background Art

[0002] Mycotoxin detection is suitable for the purification and enrichment of a variety of complex samples, including grains, foods, feeds, condiments, Chinese medicinal materials, etc. It is suitable for aflatoxins, vomitoxin (deoxynivalenol), fumonisins, zearalenone, ochratoxin, and T-2 toxin.

[0003] Before testing, samples must be processed using a cleanup column. This column contains adsorbents (including polar, non-polar, and ion exchange functional groups) that trap and adsorb impurities such as lipids, proteins, and pigments. The target substance then passes through the column along with the sample, resulting in purification and enrichment. The resulting liquid is then used directly for HPLC and LC-MS / MS analysis. However, traditional cleanup columns can only process one sample at a time. This is a time-consuming and inconvenient process when processing a large number of samples.

[0004] Therefore, we propose a pretreatment cleanup column for the detection of multiple mycotoxins. Utility Model Content

[0005] The purpose of the utility model is to solve the shortcomings of the prior art that the purification column can only process one sample liquid at a time, and when more sample liquids need to be processed, it takes a lot of time and energy and is relatively inconvenient. A purification column for pre-treatment of multiple fungal toxins is proposed.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A pre-treatment cleanup column for various mycotoxins detection, including:

[0008] A frame, wherein a fixing plate is fixed between the inner walls of both sides of the frame, a plurality of through holes are formed through the surface of the fixing plate, a syringe is provided in each through hole, and an adsorption filler is provided on the inner side of the syringe;

[0009] A motor and a controller are fixedly installed on the surface of the frame, one end of the motor output shaft passes through the frame and is fixed with a screw, the bottom end of the screw is rotatably connected to the surface of the fixed plate, a lifting plate is slidably connected between the inner walls on both sides of the frame, the screw thread passes through the lifting plate, and a plurality of extrusion mechanisms are provided at the bottom of the lifting plate for cooperating with the syringe.

[0010] In a possible design, the extrusion mechanism includes a fixed block, which is fixed to the bottom of the lifting plate. The bottom end of the fixed block is threadedly connected to a connecting rod, and the bottom end of the connecting rod is fixed with a piston.

[0011] In a possible design, a plurality of grooves are formed on the outer wall of the connecting rod.

[0012] In a possible design, a plurality of positioning tubes are fixedly provided at the bottom of the fixing plate, the plurality of positioning tubes are respectively connected to the plurality of through holes, and a clearance opening is provided at the bottom of the positioning tubes.

[0013] In a possible design, an observation port is provided on the outer wall of the positioning tube.

[0014] In a possible design, a plurality of placement grooves are provided on the inner wall of the bottom of the frame, and the placement grooves are located below the through holes.

[0015] In a possible design, a plurality of rubber pads are fixedly provided on the bottom of the frame.

[0016] In this application, when in use, the adsorption filler is placed in the syringe, and then an appropriate amount of sample liquid is added to the syringe, and then the syringe is placed in the through hole, and then the collection container is placed in the placement slot, and then the motor is started by the controller, the motor drives the screw to rotate, the screw drives the lifting plate to descend, the lifting plate drives the fixed block, the connecting rod and the piston to descend, the piston enters the syringe and then squeezes the sample liquid, so that the target passes through the adsorption filler and falls into the collection container to complete the collection, and then the motor is started to drive the lifting plate to reset, the piston drives the syringe to rise and disengage from the through hole, and then the syringe and piston are removed for cleaning.

[0017] Beneficial effects: In the present invention, the purification column for pretreatment of multiple mycotoxins detection can process multiple sample solutions at one time through the arrangement of multiple structures such as a motor, a screw, and a lifting plate, and does not require human power, thus saving the time and energy of the experimenter.

[0018] In the present invention, the pretreatment purification column for multiple mycotoxin detection is provided with a fixed block, a connecting rod, a piston and other structures. The connecting rod and the syringe are both disposable items. After use, the experimenter can conveniently remove and discard them, avoiding the harm of toxins to the human body and cross contamination during the reuse and cleaning process.

[0019] In the present invention, multiple sample liquids can be processed at one time without the need for human effort, saving the time and energy of the experimenter. The connecting rod and syringe are both disposable items, which can be conveniently removed and discarded by the experimenter after use, avoiding the harm of toxins to the human body and cross contamination during the reuse and cleaning process. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall three-dimensional structure of a pre-treatment purification column for multiple mycotoxin detection proposed in the utility model;

[0021] Figure 2 This is a partial cross-sectional structural schematic diagram from a first perspective of a pre-treatment purification column for multiple mycotoxin detection proposed by the present invention;

[0022] Figure 3 This is a partial cross-sectional structural diagram from a second perspective of a pre-treatment purification column for multiple mycotoxin detection proposed by the present invention;

[0023] Figure 4 This is a schematic diagram of a partial three-dimensional structure of a pretreatment purification column for detecting multiple mycotoxins proposed in the present invention.

[0024] In the figure: 1. frame; 2. controller; 3. motor; 4. screw; 5. lifting plate; 6. placement slot; 7. rubber pad; 8. fixing plate; 9. through hole; 10. syringe; 11. positioning tube; 12. observation port; 13. clearance port; 14. fixing block; 15. connecting rod; 16. piston; 17. groove; 18. adsorption filler. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0026] Example 1: Reference Figure 1-Figure 4 , a treatment and purification column, comprising:

[0027] The frame 1 has a fixed plate 8 fixed between the inner walls on both sides of the frame 1. A plurality of through holes 9 are formed on the surface of the fixed plate 8. The through holes 9 can be set to two groups of different specifications to meet the fixation and use of two types of disposable matching syringes of 6ML and 3ML respectively, or to configure two sets of equipment of corresponding specifications. A syringe 10 is provided in the through hole 9, and an adsorption filler 18 is provided on the inner side of the syringe 10, which can adsorb and purify the sample liquid so that the target passes through the adsorption filler;

[0028] A motor 3 and a controller 2 are fixedly provided on the surface of the frame 1. The controller 2 can adjust the power of the motor 3 and thereby control the dripping speed of the liquid, such as 2-3 drops / second. One end of the output shaft of the motor 3 passes through the frame 1 and is fixedly provided with a screw 4. The bottom end of the screw 4 is rotatably connected to the surface of the fixed plate 8. A lifting plate 5 is slidably connected between the inner walls on both sides of the frame 1. The screw 4 thread penetrates the lifting plate 5. When the motor 3 drives the screw 4 to rotate, the lifting plate 5 will move up and down under the drive of the screw 4. A plurality of extrusion mechanisms are provided at the bottom of the lifting plate 5 for cooperating with the syringe 10.

[0029] The extrusion mechanism includes a fixed block 14, which is fixed to the bottom of the lifting plate 5. The bottom end of the fixed block 14 is threadedly connected to a connecting rod 15, and the bottom end of the connecting rod 15 is fixed to a piston 16. When the lifting plate 5 descends, the piston 16 presses into the syringe 10 to squeeze the sample liquid, causing the target to pass through the adsorbent filler.

[0030] This application can be used in the field of mycotoxin detection, and can also be used in other fields applicable to this application.

[0031] Example 2: An improved pretreatment purification column for multiple mycotoxin detection based on Example 1, which is applied to the field of mycotoxin detection;

[0032] In another aspect of this embodiment, a plurality of grooves 17 are formed on the outer wall of the connecting rod 15. The connecting rod 15 and the syringe are disposable items, so that the experimenter can easily remove the connecting rod 17 and the piston 16 and throw them into a garbage bag.

[0033] In another aspect of this embodiment, a plurality of positioning tubes 11 are fixedly provided at the bottom of the fixed plate 8, and the plurality of positioning tubes 11 are respectively connected to the plurality of through holes 9, which can provide more support for the syringe 10 and ensure the stability of the structure. A clearance opening 13 is opened at the bottom of the positioning tube 11 to provide clearance for the needle mouth on the syringe 10.

[0034] In another aspect of this embodiment, an observation port 12 is provided on the outer wall of the positioning tube 11 to facilitate the experimenter to understand the squeezing condition of the sample liquid in the syringe.

[0035] In another aspect of this embodiment, a plurality of placement slots 6 are provided on the bottom inner wall of the frame 1 . The placement slots 6 are located below the through holes 9 , making it convenient for people to quickly place the collection containers.

[0036] In another aspect of this embodiment, a plurality of rubber pads 7 are fixedly provided on the bottom of the frame 1 , and these rubber pads 7 can play an anti-slip role.

[0037] However, as is well known to those skilled in the art, the working principles and wiring methods of the controller 2 and the motor 3 are commonplace, and are conventional means or common knowledge, and will not be elaborated here. Those skilled in the art can make any selections according to their needs or convenience.

[0038] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A pre-treatment purification column for the detection of multiple mycotoxins, characterized in that: include: A frame (1), wherein a fixing plate (8) is fixedly provided between inner walls on both sides of the frame (1), a plurality of through holes (9) are formed through the surface of the fixing plate (8), a syringe (10) is provided in the through hole (9), and an adsorption filler (18) is provided on the inner side of the syringe (10); A motor (3) and a controller (2) are fixedly provided on the surface of the frame (1); one end of the output shaft of the motor (3) passes through the frame (1) and is fixedly provided with a screw (4); the bottom end of the screw (4) is rotatably connected to the surface of the fixed plate (8); a lifting plate (5) is slidably connected between the inner walls on both sides of the frame (1); the screw (4) thread penetrates the lifting plate (5); and a plurality of extrusion mechanisms are provided at the bottom of the lifting plate (5) for cooperating with the syringe (10).

2. A pre-treatment purification column for multiple mycotoxin detection according to claim 1, characterized in that: The extrusion mechanism comprises a fixed block (14), which is fixed at the bottom of the lifting plate (5), the bottom end of the fixed block (14) is threadedly connected to a connecting rod (15), and the bottom end of the connecting rod (15) is fixed with a piston (16).

3. A pre-treatment purification column for multiple mycotoxin detection according to claim 2, characterized in that: The outer wall of the connecting rod (15) is provided with a plurality of grooves (17).

4. A pre-treatment purification column for multiple mycotoxin detection according to claim 3, characterized in that: A plurality of positioning tubes (11) are fixedly provided at the bottom of the fixing plate (8), and the plurality of positioning tubes (11) are respectively connected to the plurality of through holes (9), and a clearance opening (13) is provided at the bottom of the positioning tubes (11).

5. A pre-treatment purification column for multiple mycotoxin detection according to claim 4, characterized in that: An observation port (12) is provided on the outer wall of the positioning tube (11).

6. A pre-treatment purification column for multiple mycotoxin detection according to claim 5, characterized in that: The bottom inner wall of the frame (1) is provided with a plurality of placement grooves (6), and the placement grooves (6) are located below the through holes (9).

7. A pre-treatment purification column for multiple mycotoxin detection according to claim 6, characterized in that: A plurality of rubber pads (7) are fixedly provided on the bottom of the frame (1).

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