Filter of indexing filtration method and device for detecting sample extracted by indexing filtration method

Abstract

The utility model relates to a filter for an indexing filtration method and a device for detecting a sample extracted by the indexing filtration method, which are characterized in that the filter comprises a plurality of layers which are overlapped up and down and are respectively and sequentially overlapped up and down above a pump body, the filter comprises a shell, and a metal filter support disc, a filter membrane, a pump head and a filter pipe are arranged in the shell; the shell is formed by folding an upper shell and a lower shell, the pump head is provided with external threads, the upper shell and the lower shell are respectively and coaxially provided with a central hole matched with the pump head in a penetrating manner, the filter is detachably connected with the pump body or the lower layer of filter through the pump head, the upper end of the filter pipe is hermetically connected with the central hole of the upper shell, and the lower end of the filter pipe is inserted into the pump head. The pore diameters of the filtering membranes and the metal filtering support discs in the filters in different layers are gradually reduced from bottom to top in sequence; the device is reasonable in structure and convenient to disassemble and assemble, can directly detect microorganisms with different diameters after being sequentially filtered in a layered manner, is very convenient to operate, and is suitable for detecting the microorganisms, algae and cells of the microorganisms.

Description

Technical Field

[0001] The utility model relates to a detection device, in particular to a detection device for extracting samples using a graduated filtration method and a related filter. Background Art

[0002] Graduated filtration primarily involves filtering a medium through a filter medium, such as a screen or filter element. The core of this process is to remove impurities and retain a clean medium. This process also ensures that the pore structures of different media meet tightness requirements. Graduated filtration is commonly used in various fields, such as water treatment, air purification, and industrial production.

[0003] In the detection scenario of microorganisms, algae and their cells, microorganisms of different diameters need to be filtered and separated for more direct detection. The current method is to use membranes of different pore sizes for multiple filtrations, which is not only complicated to operate, but also causes secondary contamination and cross-contamination.

[0004] At present, there are no reports on the use of graded filtration method to extract microbial samples for testing. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a filter of a graduated filtration method with a reasonable structure and convenient disassembly and detection, and a device for extracting samples by the graduated filtration method, in view of the above technical status quo.

[0006] The technical solution adopted by the present invention to solve the above-mentioned technical problems is: a filter using a graduated filtration method, characterized in that the filter is composed of several layers stacked up and down, which are stacked up and down in sequence above the pump body, the filter comprising a shell, a metal filter support disk, a filter membrane, a pump head and a filter tube are arranged in the shell, the shell is formed into a closed whole by meshing of an upper shell and a lower shell, the pump head has an external thread, and the upper shell and the lower shell are respectively coaxially penetrated to form a center hole that cooperates with the pump head, the filter is detachably connected to the pump body or the filter of the lower layer through the pump head, the upper end of the filter tube is sealed with the center hole of the upper shell, and the lower end is inserted into the pump head, and the apertures of the filter membranes and metal filter disks in different layers of filters are gradually reduced from bottom to top.

[0007] As an improvement, the shell is cylindrical, the upper end face of the lower shell is recessed with an internal thread, and the lower end of the upper shell is convexly provided with a corresponding external thread. The upper shell and the lower shell are fixed by a spiral connection after being matched. The pump head is an externally threaded column with an annular convex edge at the upper end, and an inner hole for the filter tube to pass through is axially opened in the middle of the pump head. The inside of the inner hole of the pump head has an annular inner groove of reasonable specifications, and is equipped with a soft annular ring to facilitate quick plugging and unplugging of the filter tube. The center holes of the upper and lower shells are internally threaded holes that match the external threads of the pump head. The upper end face of the pump body is recessed with an internally threaded hole corresponding to the pump head. The pump head passes through the internal threaded hole of the lower shell and is screwed into the internal threaded hole of the pump body to connect the filter to the pump body, or, the pump head passes through the internal threaded hole of the lower shell and is screwed into the internal threaded hole of the upper shell of the lower filter to assemble and connect the upper and lower filters.

[0008] Furthermore, an annular groove is formed on the inner wall of the bottom surface of the lower housing around the internal threaded hole. The pump head passes through the internal threaded hole and is restrained by the annular ridge against the annular groove. The lower housing is equipped with a metal filter support plate for supporting the filter membrane. It can be used as a support plate for sample filtration or as an impactor plate for air filtration. The impactor is composed of multiple stages with tiny nozzle apertures.

[0009] Furthermore, a Luer interface nozzle is coaxially provided on the lower end surface of the upper shell around the position of the central hole and is sealed and docked with the upper end of the filter tube.

[0010] Furthermore, a sealing ring is provided in the inner hole of the pump head, and the lower end of the filter tube is inserted into the inner hole of the pump head and is positioned against the sealing ring.

[0011] Furthermore, the housing of the filter is a metal housing.

[0012] A sample extraction and detection device using a graduated filtration method is characterized in that it includes a box body, a pump body and a filter. A sample inlet tube is provided in the box body, the pump body is arranged on the box body and is connected to the sample inlet tube, and any one of the above-mentioned filters is used.

[0013] Furthermore, the sample injection tubes in the box body include a main injection tube and several branch injection tubes, and the branch injection tubes are respectively connected to the main injection tube. The sample injection tubes are connected to the vacuum pump in the box body through a branch component, and the other branch injection tubes are connected to the side wall of the box body. A through hole is provided for the main injection tube to extend, which is used for sample and gas filtering respectively. The upper end surface of the box body is provided with an opening corresponding to the branch injection tube, and a flange is welded at the opening to connect with the branch injection tube. The lower end of the pump body is provided with an external threaded connecting pipe that matches the flange. The pump body is detachably mounted on the box body by a threaded connection and is connected to the branch injection tube. The side wall of the box body is provided with a through hole for the main injection tube to extend.

[0014] Finally, there are 2 to N sample injection pipes, corresponding to 2 to N pump bodies, each of which is equipped with a valve, where N is a natural number.

[0015] Compared with the prior art, the advantages of the present invention are as follows: the present device is designed for detecting different filtered objects, multiple layers of filters are sequentially arranged on the pump body, and the pore size of the filter membrane in each layer of filter changes from large to small. When the sample passes through the filter, it can intercept microorganisms, algae and cells of different diameters for detection, so that microorganisms, algae and cells of different diameters can be obtained under the same test, avoiding secondary contamination and cross contamination; the filter is connected to the pump body and the filter through the pump head, which is very convenient to disassemble and assemble; the shell is made of metal, which is convenient for sterilization and disinfection, and the upper shell is provided with a Luer interface nozzle to cooperate with the filter tube, with good sealing performance; a sealing ring is provided in the pump head, which is convenient for quick insertion and removal of the filter tube; the lower shell is provided with a metal filter support plate as a support, which is convenient for using various filter membranes of different pore sizes on the market. Therefore, the present structure is suitable for filtering organic matter and inorganic matter in a certain state, and is also suitable for gaseous multi-stage sieve hole impact-type air microbial sampling. The present invention has a reasonable structure and is easy to disassemble and assemble. It can directly detect microorganisms, algae and cells of different diameters after layered filtration, and is very convenient to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a structural diagram of an embodiment of the utility model (the pump body is not installed);

[0017] Figure 2 This is a schematic diagram of the structure of the embodiment of the utility model in the detection state (with the pump body installed);

[0018] Figure 3 This is a partial exploded view of an embodiment of the utility model (the pump body is not installed);

[0019] Figure 4 This is a partial exploded view of an embodiment of the utility model (the pump body is not installed);

[0020] Figure 5 for Figure 1 Structural cross-sectional view;

[0021] Figure 6 This is a schematic structural diagram of an embodiment of the utility model after removing the upper cover of the box body (with the pump body installed). DETAILED DESCRIPTION

[0022] The present invention will be described in further detail below with reference to the accompanying drawings and embodiments.

[0023] like Figures 1 to 6As shown, a sample extraction and detection device using a graduated filtration method includes a housing 1, a pump body 2 and a filter A. A sample inlet tube is provided in the housing 1. The pump body 2 is arranged on the housing 1 and is connected to the sample inlet tube. The filter A is a filter using a graduated filtration method. The filter A is composed of several layers stacked up and down, which are stacked up and down in sequence above the pump body 2. The filter A includes a shell, a metal filter support disk, a filter membrane, a pump head 5 and a filter tube 6 are provided in the shell, and the shell is formed by an upper shell 3 and a lower shell 4. The pump head 5 has an external thread, and the upper shell 3 and the lower shell 4 are coaxially penetrated and provided with center holes 32 and 42 respectively that cooperate with the pump head 5. The filter A is detachably connected to the pump body 2 or the filter A below through the pump head 5. The upper end of the filter tube 6 is sealed with the center hole of the upper shell 3, and the lower end is inserted into the pump head 5. The pore size of the filter membrane and its metal filter support disk in different layers of filters A gradually decreases from bottom to top.

[0024] The specific structure is as follows: the shell of the filter A is a cylindrical metal shell, which is convenient for sterilization and disinfection; the upper end surface of the lower shell 4 is recessed with an internal thread 41, and the lower end of the upper shell 3 is convexly provided with a corresponding external thread 31. The meshing teeth of the upper shell 3 and the lower shell 4 are sealed and fixed by a spiral connection. The pump head 5 is an externally threaded column with an annular convex edge at the upper end, and an inner hole for the filter tube 6 to pass through is axially opened in the middle part of the pump head 5. The center holes 32 and 42 of the upper shell 3 and the lower shell 4 are internally threaded holes that cooperate with the external threads of the pump head 5. The upper end surface of the pump body 2 is recessed with an internally threaded hole corresponding to the pump head 5. The pump head 5 passes through the internally threaded hole 42 of the lower shell 4 and is screwed into the internally threaded hole of the pump body 2 to connect the filter A to the pump body 2, or, the pump head 5 passes through the internally threaded hole 42 of the lower shell 4 and is screwed into the internally threaded hole 32 of the upper shell 3 of the lower filter A to assemble and connect the upper and lower layers of filters A. An annular groove is formed on the inner wall of the bottom surface of the lower housing 4, surrounding the internally threaded hole 42. The pump head 5 passes through the internally threaded hole 42 and is restrained by an annular ridge against the groove. A metal filter support plate is located within the lower housing 4, supporting the filter membrane. The filter membrane is clamped in place by the upper and lower housings 3 and 4. A Luer port 33 is coaxially projected on the lower end surface of the upper housing 3, surrounding the center hole 32, and sealably engages with the upper end of the filter tube 6. A sealing ring 7 is located within the inner bore of the pump head 5. The lower end of the filter tube 6 is inserted into the inner bore of the pump head 5, with the outer wall of the filter tube 6 abutting against the sealing ring 7.

[0025] The box body 1 has a right-angled trapezoidal structure. The sampling pipes in the box body 1 include a sampling main pipe 8 and several sampling branch pipes 9. The sampling branch pipes 9 are respectively connected to the sampling main pipe 8. The side wall of the box body 1 is provided with a through hole for the sampling main pipe 8 to extend out. The upper end surface of the box body 1 is provided with an opening corresponding to the sampling branch pipe 9. A flange is provided at the opening to connect with the sampling branch pipe 9. The lower end of the pump body 2 is provided with an externally threaded connecting pipe that cooperates with the flange. The pump body 2 is detachably mounted on the box body 1 by a threaded connection and is connected to the sampling branch pipe 9; a valve 20 is installed on the pump body 2.

[0026] In this embodiment, there are three sampling branches 9, and correspondingly, there are three pump bodies 2. One or two groups of detection can be performed as needed, or three groups of parallel detection can be performed simultaneously. The filter A set on the pump body 2 is three-layered, and a valve 20 is installed on the pump body 2.

[0027] During operation, first install the pump body 2 and filter A. The sample enters the sample feed pipe 8 through the sample feed pipe 9. Under the action of the pump body 2, the sample enters the filter A upward. The three layers of filter A with different pore sizes filter the microorganisms of different diameters in turn, and then intercepts them for detection. In this way, microorganisms of different diameters can be obtained under the same sample, avoiding secondary contamination and cross contamination.

[0028] The advantages of using the detection device of the utility model for graded filtration are:

[0029] 1. The graduated filtration process can retain 1.0um somatic cells / animal cells and other non-bacterial cells on the first layer through filter membranes with different pore sizes;

[0030] 2. The filtration process can eliminate substances such as salt and heavy metals;

[0031] 3. The total number of yeast and molds and the total number of spores of 0.45um can be filtered to the second layer;

[0032] 4. The third layer uses a 0.22um pore size to trap bacteria. When using the following punching holes in the metal filter support plate, the anatomical structure and aerodynamic characteristics of the human respiratory tract can be simulated. Using the conventional impact principle, microbial particles suspended in the air are collected on the surface of the sampling medium according to size. Then, co-culture and further microbial analysis are performed to determine the number of airborne microbial particles and their size distribution characteristics, and the capture particle range is:

[0033]

[0034] That is, the sample can be collected by agar culture medium, or by filter tube method and then rapidly detected by optical biological detection method ATP method.

[0035] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A filter using a graduated filtration method, characterized in that: The filter is composed of several layers stacked up and down, which are stacked up and down in sequence above the pump body. The filter includes a shell, in which a metal filter support plate, a filter membrane, a pump head and a filter tube are arranged. The shell is formed by the upper shell and the lower shell. The pump head has an external thread. The upper shell and the lower shell are coaxially penetrated and a center hole is opened to match the pump head. The filter is detachably connected to the pump body or the filter below through the pump head. The upper end of the filter tube is sealed with the center hole of the upper shell, and the lower end is inserted into the pump head. The apertures of the filter membranes and their metal filter support plates in different layers of filters gradually decrease from bottom to top.

2. The filter of the graduated filtration method according to claim 1, characterized in that: The shell is cylindrical, the upper end surface of the lower shell is concavely provided with an internal thread, and the lower end of the upper shell is convexly provided with a corresponding external thread. The upper shell and the lower shell are fixed by a spiral connection after being matched. The pump head is an externally threaded column with an annular convex edge at the upper end, and an inner hole for the filter tube to pass through is axially opened in the middle of the pump head. The center holes of the upper shell and the lower shell are internally threaded holes that match the external threads of the pump head. The upper end surface of the pump body is concavely provided with an internally threaded hole corresponding to the pump head. The pump head passes through the internally threaded hole of the lower shell and is screwed into the internally threaded hole of the pump body to connect the filter to the pump body, or, the pump head passes through the internally threaded hole of the lower shell and is screwed into the internally threaded hole of the upper shell of the lower filter to assemble and connect the upper and lower filters.

3. The filter of the graduated filtration method according to claim 2, characterized in that: An annular groove is formed on the inner wall of the bottom surface of the lower shell around the internal threaded hole. The pump head passes through the internal threaded hole and is limited by the annular convex edge and the annular groove. A metal filter support plate for supporting the filter membrane is provided in the lower shell.

4. The filter of the graduated filtration method according to claim 3, characterized in that: The lower end surface of the upper shell is coaxially provided with a Luer interface mouth around the position of the central hole, which is sealed and connected to the upper end of the filter tube.

5. The filter of the graduated filtration method according to claim 4, characterized in that: A sealing ring is provided in the inner hole of the pump head, and the lower end of the filter tube is inserted into the inner hole of the pump head and is positioned against the sealing ring.

6. The filter for the graduated filtration method according to any one of claims 1 to 5, characterized in that: The housing of the filter is a metal housing.

7. A sample extraction and detection device using a graduated filtration method, characterized in that: The invention comprises a box body, a pump body and a filter. The box body is provided with a sampling tube. The pump body is arranged on the box body and is connected with the sampling tube. The filter according to any one of claims 1 to 6 is adopted.

8. The sample extraction and detection device using the graduated filtration method according to claim 7, characterized in that: The sampling tubes in the box body include a main sampling tube and several branch sampling tubes, which are respectively connected to the main sampling tube. The side wall of the box body is provided with a through hole for the main sampling tube to extend out, and the upper end surface of the box body is provided with an opening corresponding to the branch sampling tube. A flange is provided at the opening to connect with the branch sampling tube. The lower end of the pump body is provided with an external threaded connecting pipe that matches the flange. The pump body is detachably mounted on the box body by a threaded connection and is connected to the branch sampling tube.

9. The sample extraction and detection device using the graduated filtration method according to claim 8, characterized in that: There are 2 to N sample injection pipes, corresponding to 2 to N pump bodies, each of which is equipped with a valve, and N is a natural number.

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