Stem cell filtering and separating device

Abstract

The utility model provides a stem cell filtering and separating device. The stem cell filtering and separating device comprises a bottom cylinder and a cylinder cover, a filter cylinder is arranged in the bottom cylinder; filter paper is detachably placed on the inner side of the filter cartridge, the cartridge bottom of the filter cartridge is communicated with the top of the liquid storage cartridge, and a liquid discharge pipe is arranged at the bottom of the liquid storage cartridge; the liquid storage cylinder is connected with the upper part of the liquid storage connecting rod and is slidably connected with the bottom cylinder through the liquid storage connecting rod; the lower part of the liquid storage connecting rod is movably connected with one end of the lever plate in the length direction; the lever plate is hinged to the bottom cylinder, and the other end of the lever plate movably penetrates through the bottom cylinder and is movably connected with the bottom of the driving rod on the outer side of the bottom cylinder. One end of the cylinder cover is hinged to the bottom cylinder, the hinged position is arranged at the end where the driving rod is located, and the other end of the cylinder cover is detachably connected with the bottom cylinder. An annular water pipe is arranged on the inner side of the cylinder cover, and multiple nozzles are arranged on the inner side of the annular water pipe at intervals. According to the device, by changing the placing structure of the filter paper, an operator can better replace the filter paper and stem cell tissues, and the stem cell tissues can be protected while the stem cell filtering and separating effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell filtration, in particular to a stem cell filtration separation device. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. They are called "universal cells" in the medical community. Therefore, the research on stem cells is of great significance. Among them, the separation technology of stem cells is also increasingly valued in the research on stem cells.

[0003] Most existing stem cell separation devices use filtration separation. To increase separation efficiency, some stem cell separation devices are equipped with stirring devices. For example, the stem cell separation device provided by Chinese Utility Model Patent Application No. CN202120551749.0 achieves stirring by rotating a shaft, thereby increasing separation efficiency. However, in cases where stirring is believed to damage stem cells, stirring can sometimes affect the extraction effect. Chinese Utility Model Patent Application No. CN202222893645.5 provides a stem cell filtration separation device that uses a swirling flow to flush the stem cell tissue, causing the stem cells on the filter paper to be disturbed while being flushed evenly and slowly. This improves separation efficiency to a certain extent, while also protecting the stem cell tissue and achieving better extraction results. However, while this patent uses a motor to drive the drainage box up and down, it utilizes the telescopic action of the electric push rod to tilt the drainage box toward the opening of the main cylinder, making it easier for the operator to replace the filter paper and stem cell tissue. This method is too complex in structure, and because the drainage box remains within the main cylinder, the replacement operation is not very convenient for the operator.

[0004] Therefore, the existing stem cell filtration device still has some shortcomings and needs to be improved. Utility Model Content

[0005] In view of the above, it is necessary to provide a stem cell filtration separation device that retains the cyclonic flushing function and, by changing the structure of the filter paper placement, enables the operator to better replace the filter paper and stem cell tissue. This device improves the stem cell filtration separation effect while protecting the stem cell tissue and facilitating operation for the operator.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted by the present utility model is:

[0007] A stem cell filtration separation device comprises a bottom cylinder and a cylinder cover installed on the top of the bottom cylinder;

[0008] The bottom cylinder is provided with a filter cylinder, a liquid storage cylinder and a liquid storage connecting rod; a filter paper is detachably placed on the inner side of the filter cylinder, and the upper part of the filter paper is sleeved on the open top edge of the filter cylinder; the bottom of the filter cylinder is connected to the top of the liquid storage cylinder, and a drain pipe is provided at the bottom of the liquid storage cylinder, and the end of the drain pipe that is not connected to the liquid storage cylinder passes through the bottom cylinder and extends to the outside of the bottom cylinder, and a drain valve is provided on the outside of the bottom cylinder; the liquid storage cylinder is connected to the upper part of the liquid storage connecting rod, and is slidably connected to the bottom cylinder through the liquid storage connecting rod, and the sliding direction is the up and down direction of the bottom cylinder; the lower part of the liquid storage connecting rod extends The lever plate is hinged to the bottom of the liquid storage cylinder, and the hinge axis is perpendicular to the up-down direction of the bottom cylinder and the length direction of the lever plate. The other end of the lever plate is movably inserted into the bottom cylinder and is movably connected to the bottom of the driving rod on the outside of the bottom cylinder. The upper part of the driving rod is movably inserted into the limiting ring outside the bottom cylinder, and the top of the driving rod is located above the limiting ring. The gravity of the driving rod is less than the gravity of the liquid storage cylinder. In a natural state, the lever plate sets one end of the liquid storage cylinder to sink, and the upper end of the driving rod extends above the bottom cylinder.

[0009] One end of the cylinder cover is hinged to the bottom cylinder, and the hinge is provided at the end where the drive rod is located. The other end of the cylinder cover is detachably connected to the bottom cylinder. An annular water pipe is provided on the inner side of the cylinder cover, and a plurality of nozzles are provided at intervals on the inner side of the annular water pipe, and the orientations of all the nozzles are at the same angle to the radial direction of the annular water pipe. The annular water pipe is connected to the outlet end of the liquid pump, and the liquid pump is installed on the cylinder cover. The inlet end of the liquid pump is connected to the external liquid storage tank through a liquid inlet pipe, and the end of the liquid inlet pipe not connected to the liquid pump is located on the outer side of the cylinder cover.

[0010] When the cylinder cover covers the bottom cylinder, the annular water pipe is located directly above the top of the filter cylinder; when the cylinder cover opens the bottom cylinder, the outside of the cylinder cover abuts against the upper end of the drive rod, and the filter cylinder protrudes from the top of the bottom cylinder.

[0011] Preferably, the filter cartridge and the filter paper are both funnel-shaped, and the top outer wall of the filter paper has an annular groove that is buckled with the top edge of the filter cartridge.

[0012] Preferably, the portion of the liquid discharge pipe located in the bottom cylinder is a hose.

[0013] Preferably, the liquid storage connecting rod is movably connected to the lever plate through a roller.

[0014] Preferably, the lower end of the driving rod is hinged to the lever plate, and the upper end surface is a convex arc surface structure.

[0015] Preferably, the liquid pump is arranged on the outer side of the cylinder cover.

[0016] Preferably, the outer frame of the annular water pipe is provided with a water baffle, the lower part of the water baffle extends to the bottom of the annular water pipe, the lower part of the water baffle is funnel-shaped, and the lower end of the water baffle is inserted into the filter cylinder when the cylinder cover is closed on the bottom cylinder, and abuts the filter paper.

[0017] Preferably, the lower end edge of the water baffle is wrapped with anti-slip silica gel.

[0018] Preferably, a rotating motor is installed on the outer side of the barrel cover, the output shaft of the rotating motor is connected to the rotating shaft, the rotating shaft is rotatably inserted on the barrel cover, and is coaxially arranged with the annular water pipe. The rotating shaft is located on one end of the inner side of the barrel cover and is detachably installed with a stirring shaft, and a stirring blade is installed on the stirring shaft.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The utility model adopts the arrangement of the liquid storage connecting rod, the lever plate, and the driving rod, so that the liquid storage cylinder and the filter cylinder connected thereto can be raised and lowered, so that when the cylinder cover is opened, the liquid storage cylinder and the filter paper are automatically raised to expose the filter cylinder and the filter paper, which is convenient for the operator to replace the filter paper and the stem cell tissue fluid. Moreover, the lifting of the utility model is mechanical-free, with a simple structure and easy operation.

[0021] 2. The utility model realizes rotary spraying of the filtrate through an annular water pipe and a nozzle arranged in an annular manner thereon, so as to disturb the stem cell tissue fluid by means of rotary disturbance, facilitate filtration and separation, improve separation effect, and protect stem cell tissue at the same time.

[0022] 3. The utility model provides a rotating motor, a rotating shaft, a stirring shaft and a stirring blade on the barrel cover, so that the device has the function of stirring, filtering and separating. The stirring shaft and the rotating shaft are detachably connected, so that the stirring and separation function can be installed according to actual operation requirements, thereby achieving the desired filtration separation of stem cell tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of the utility model when in use.

[0024] Figure 2 It is a structural schematic diagram of the utility model when the cylinder cover is opened.

[0025] Description of main component symbols

[0026] In the figure: bottom cylinder 1, cylinder cover 2, filter cylinder 3, filter paper 4, liquid storage cylinder 5, discharge pipe 6, liquid storage connecting rod 7, lever plate 8, drive rod 9, limit ring 10, annular water pipe 11, nozzle 12, liquid pump 13, rotating motor 14, rotating shaft 15, stirring shaft 16, stirring blade 17.

[0027] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0028] See also Figure 1-2 In a preferred embodiment of the present invention, a stem cell filtration separation device includes a bottom cylinder 1 and a cylinder cover 2 installed on the top of the bottom cylinder 1.

[0029] The bottom cylinder 1 is provided with a filter cylinder 3, a liquid storage cylinder 5 and a liquid storage connecting rod 7; a filter paper 4 is detachably placed on the inner side of the filter cylinder 3, and the upper part of the filter paper 4 is sleeved on the open top edge of the filter cylinder 3; the bottom of the filter cylinder 3 is connected to the top of the liquid storage cylinder 5, and a drain pipe 6 is provided at the bottom of the liquid storage cylinder 5, and the end of the drain pipe 6 not connected to the liquid storage cylinder 5 passes through the bottom cylinder 1 and extends to the outside of the bottom cylinder 1, and a drain valve (not shown) is provided on the outside of the bottom cylinder 1; the liquid storage cylinder 5 is connected to the upper part of the liquid storage connecting rod 7, and is slidably connected to the bottom cylinder 1 through the liquid storage connecting rod 7, and the sliding direction is the up and down direction of the bottom cylinder 1; the liquid storage connecting rod 7 The lower part extends to the bottom of the liquid storage cylinder 5 and is movably connected to one end of the lever plate 8 in the length direction; the lever plate 8 is hinged to the bottom cylinder 1, and the axis of the hinge is perpendicular to the up and down direction of the bottom cylinder 1 and the length direction of the lever plate 8. The other end of the lever plate 8 movably passes through the bottom cylinder 1 and is movably connected to the bottom of the driving rod 9 on the outside of the bottom cylinder 1; the upper part of the driving rod 9 is movably inserted into the limiting ring 10 outside the bottom cylinder 1, and its top is located above the limiting ring 10; the gravity of the driving rod 9 is less than the gravity of the liquid storage cylinder 5. In a natural state, the lever plate 8 sets one end of the liquid storage cylinder 5 to sink, and the upper end of the driving rod 9 extends above the bottom cylinder 1;

[0030] One end of the cylinder cover 2 is hinged to the bottom cylinder 1, and the hinge is arranged at the end where the drive rod 9 is located. The other end of the cylinder cover 2 is detachably connected to the bottom cylinder 1, for example, by a buckle buckle connection; an annular water pipe 11 is provided on the inner side of the cylinder cover 2, and a plurality of nozzles 12 are arranged at intervals on the inner side of the annular water pipe 11, and the directions of all the nozzles 12 are at the same angle to the radial direction of the annular water pipe 11; the annular water pipe 11 is connected to the outlet end of the liquid pump 13, and the liquid pump 13 is installed on the cylinder cover 2. The inlet end of the liquid pump 13 is connected to an external liquid storage tank (not shown) through a liquid inlet pipe, and the end of the liquid inlet pipe not connected to the liquid pump 13 is located outside the cylinder cover 2;

[0031] When the cylinder cover 2 covers the bottom cylinder 1, the annular water pipe 11 is located directly above the top of the filter cylinder 3; when the cylinder cover 2 opens the bottom cylinder 1, the outside of the cylinder cover 2 abuts against the upper end of the drive rod 9, and the filter cylinder 3 protrudes from the top of the bottom cylinder 1.

[0032] That is, the present invention achieves stem cell separation by filtering through the filter paper 4 on the filter cartridge 3. In this embodiment, in order to facilitate the extraction of tissue fluid after stem cell separation, it is preferred that the filter cartridge 3 and the filter paper 4 are both funnel-shaped, and the top outer wall of the filter paper 4 has an annular groove that is engaged with the top edge of the filter cartridge 3. The filter paper 4 is engaged with the top of the filter cartridge 3 through the annular groove to prevent the filter paper 4 from shifting during the separation process.

[0033] In the present invention, the filtration separation of stem cell tissue is achieved by flushing with the filtrate sprayed from multiple nozzles 12 through the annular water pipe 11 above the filter cartridge 3. The filtrate can be rotated, thereby disturbing the stem cell tissue on the filter paper 4, making it easier to filter and separate the stem cell tissue. Compared with filtration with only a filter mesh, the filtration effect is better and the extraction effect is also better. In this embodiment, in order to allow the spray water ejected from the annular water pipe 11 to better act on the filter cartridge 3 and prevent the spray water from splashing, a water baffle is preferably provided on the outer frame of the annular water pipe 11. The lower portion of the water baffle extends below the annular water pipe 11. The lower portion of the water baffle is funnel-shaped. The lower end of the water baffle is inserted into the filter cartridge 3 when the cylinder cover 2 covers the bottom cylinder 1 and abuts the filter paper 4. In this way, the cylinder cover 2, the water baffle, the filter cartridge 3 and the liquid storage cylinder 5 form a relatively closed space. The spray water can only exist in this space and cannot splash out, which is very good for collecting the spray water. At the same time, the way the water baffle abuts the filter paper 4 can also fix the filter paper 4, further preventing the filter paper 4 from shifting. Furthermore, the lower end of the water baffle is wrapped with non-slip silicone to achieve stable abutment with the filter paper 4 while avoiding damage to the filter paper 4. In addition, in this embodiment, the liquid pump 13 is provided on the outer surface of the cylinder cover 2.

[0034] After the stem cell tissue of the present invention is filtered and separated by spraying water, the filtered liquid enters the liquid storage cylinder 5 and is discharged through the drainage pipe 6 at the bottom of the liquid storage cylinder 5 for centralized collection. Considering that the liquid storage cylinder 5 of the present application can be raised and lowered under the action of the lever plate 8, this embodiment configures the portion of the drainage pipe 6 located within the bottom cylinder 1 as a hose. In addition, to enable the liquid storage cylinder 5 to be more effectively raised and lowered in response to the rotation of the lever plate 8, the liquid storage connecting rod 7 is preferably movably connected to the lever plate 8 via a roller. The roller is connected to the lever plate 8 and can roll back and forth on the lever plate 8 along the length of the lever plate 8. This ensures that when the lever plate 8 rotates, the liquid storage connecting rod 7 only rises and falls without swinging left and right, thereby ensuring that the liquid storage cylinder 5 rises and falls in a directional manner.

[0035] The directional lifting of the liquid storage cylinder 5 of the utility model is achieved by utilizing the principle of leverage and gravity. Specifically, the weight of the liquid storage cylinder 5, the liquid storage connecting rod 7, the filter cylinder 3, etc. arranged at one end of the lever plate 8 is heavier than the weight of the driving rod 9 at the other end of the lever plate 8. Under the action of gravity, the end of the lever plate 8 where the liquid storage cylinder 5 is set sinks, and the liquid storage cylinder 5 and the filter cylinder 3 descend to the inside of the bottom cylinder 1 and do not protrude above the top of the bottom cylinder 1, while the other end of the lever plate 8 where the driving rod 9 is set rises, and the driving rod 9 rises. When the cylinder cover 2 is opened, the cylinder cover 2 flips and contacts the drive rod 9, gradually pressing the drive rod 9 downward. The drive rod 9 is pressed down and drives the lever plate 8 to rotate, causing the end of the lever plate 8 where the liquid storage cylinder 5 is located to rise. The liquid storage cylinder 5 and the filter cylinder 3 also rise accordingly, so that the filter cylinder 3 protrudes from the top of the bottom cylinder 1, exposing the filter paper 4. In this way, the operator can easily replace the filter paper 4 and the stem cell tissue. In addition, as can be seen from the above, the lifting and lowering of the filter cylinder 3 and the liquid storage cylinder 5 of the utility model do not require a power part, and its structure is simple and easy to operate. In this embodiment, in order to facilitate the cylinder cover 2 to press the drive rod 9 downward, the lower end of the drive rod 9 is hinged to the lever plate 8, and the upper end surface is a convex curved surface structure. The cylinder cover 2 contacts the drive rod 9 through the curved surface structure. The drive rod 9 is pressed downward by the cylinder cover 2, and when pressed downward, the drive rod 9 is limited by the setting of the stop ring 10 and can only move downward and move slightly along the length direction of the lever plate 8.

[0036] Furthermore, in this embodiment, a rotating motor 14 is installed on the outer side of the barrel cover 2, and the output shaft of the rotating motor 14 is connected to a rotating shaft 15. The rotating shaft 15 is rotatably inserted into the barrel cover 2 and is coaxially arranged with the annular water pipe 11. The rotating shaft 15 is located at one end of the inner side of the barrel cover 2 and is detachably mounted with a stirring shaft 16. A stirring blade 17 is installed on the stirring shaft 16. The stirring blade 17 extends into the filter barrel 3 when the barrel cover 2 covers the bottom barrel 1. When the rotating motor 14 drives the rotating shaft 15 and the stirring shaft 16 to rotate, the stem cell tissue fluid in the filter barrel 3 is stirred, further improving the efficiency and effect of the separation. Secondly, the detachable connection between the stirring shaft 16 and the rotating shaft 15 can meet the separation use under different requirements. That is to say, when there is a concern that stirring the stem cell tissue fluid will destroy the stem cells, the stirring blade 17 can be removed and only the swirl spray method can be used for filtration and separation. That is, through the above-mentioned setting, this device can have multiple functional effects and its practicality is stronger.

[0037] Finally, it should be noted that after the cylinder cover 2 covers the bottom cylinder 1, the annular water pipe 11, the filter cylinder 3 and the liquid storage cylinder 5 are coaxially arranged.

[0038] The above description is a detailed description of the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the patent application of the present invention. All equivalent changes or modifications completed under the technical spirit suggested by the present invention should fall within the patent scope covered by the present invention.

Claims

1. A stem cell filtration separation device comprising a bottom cylinder and a cylinder cover mounted on top of the bottom cylinder; characterized in that: The bottom cylinder is provided with a filter cylinder, a liquid storage cylinder and a liquid storage connecting rod; a filter paper is detachably placed on the inner side of the filter cylinder, and the upper part of the filter paper is sleeved on the open top edge of the filter cylinder; the bottom of the filter cylinder is connected to the top of the liquid storage cylinder, and a drain pipe is provided at the bottom of the liquid storage cylinder, and the end of the drain pipe that is not connected to the liquid storage cylinder passes through the bottom cylinder and extends to the outside of the bottom cylinder, and a drain valve is provided on the outside of the bottom cylinder; the liquid storage cylinder is connected to the upper part of the liquid storage connecting rod, and is slidably connected to the bottom cylinder through the liquid storage connecting rod, and the sliding direction is the up and down direction of the bottom cylinder; the lower part of the liquid storage connecting rod extends The lever plate is hinged to the bottom of the liquid storage cylinder, and the hinge axis is perpendicular to the up-down direction of the bottom cylinder and the length direction of the lever plate. The other end of the lever plate is movably inserted into the bottom cylinder and is movably connected to the bottom of the driving rod on the outside of the bottom cylinder. The upper part of the driving rod is movably inserted into the limiting ring outside the bottom cylinder, and the top of the driving rod is located above the limiting ring. The gravity of the driving rod is less than the gravity of the liquid storage cylinder. In a natural state, the lever plate sets one end of the liquid storage cylinder to sink, and the upper end of the driving rod extends above the bottom cylinder. One end of the cylinder cover is hinged to the bottom cylinder, and the hinge is provided at the end where the drive rod is located. The other end of the cylinder cover is detachably connected to the bottom cylinder. An annular water pipe is provided on the inner side of the cylinder cover, and a plurality of nozzles are provided at intervals on the inner side of the annular water pipe, and the orientations of all the nozzles are at the same angle to the radial direction of the annular water pipe. The annular water pipe is connected to the outlet end of the liquid pump, and the liquid pump is installed on the cylinder cover. The inlet end of the liquid pump is connected to the external liquid storage tank through a liquid inlet pipe, and the end of the liquid inlet pipe not connected to the liquid pump is located on the outer side of the cylinder cover. When the cylinder cover covers the bottom cylinder, the annular water pipe is located directly above the top of the filter cylinder; when the cylinder cover opens the bottom cylinder, the outside of the cylinder cover abuts against the upper end of the drive rod, and the filter cylinder protrudes from the top of the bottom cylinder.

2. The stem cell filtration separation device according to claim 1, wherein: The filter cartridge and the filter paper are both funnel-shaped, and the top outer wall of the filter paper is provided with an annular groove for buckling the top edge of the filter cartridge.

3. The stem cell filtration separation device according to claim 1, wherein: The portion of the liquid discharge pipe located in the bottom cylinder is a hose.

4. The stem cell filtration separation device according to claim 1, wherein: The liquid storage connecting rod is movably connected to the lever plate through a roller.

5. The stem cell filtration separation device according to claim 1, wherein: The lower end of the driving rod is hinged to the lever plate, and the upper end surface is a convex arc surface structure.

6. The stem cell filtration separation device according to claim 1, wherein: The liquid pump is arranged on the outer side of the cylinder cover.

7. The stem cell filtration separation device according to claim 1, wherein: The outer frame of the annular water pipe is provided with a water baffle, the lower part of the water baffle extends to the bottom of the annular water pipe, the lower part of the water baffle is funnel-shaped, and the lower end of the water baffle is inserted into the filter cylinder when the cylinder cover covers the bottom cylinder and abuts the filter paper.

8. The stem cell filtration separation device according to claim 7, wherein: The lower end edge of the water baffle is wrapped with anti-slip silica gel.

9. The stem cell filtration separation device according to claim 1, wherein: A rotating motor is installed on the outer side of the cylinder cover, and the output shaft of the rotating motor is connected to the rotating shaft. The rotating shaft is rotatably inserted on the cylinder cover and is coaxially arranged with the annular water pipe. A stirring shaft is detachably installed on one end of the rotating shaft located on the inner side of the cylinder cover, and a stirring blade is installed on the stirring shaft.

Images