Device system for liquid tissue culture of aseptic seedlings

Abstract

The utility model relates to the technical field of culture of aseptic seedlings, in particular to a device system for liquid tissue culture of aseptic seedlings, which comprises a culture dish, a liquid tissue culture device and a liquid tissue culture device, a vertical rod is arranged on the surface of the high-density and high-water-absorption polyester fiber board, an extension plate is arranged at the end part of the vertical rod, a cover body is arranged at the top of the culture dish, an air pipe and a through groove are arranged on the surface of the cover body, and a clamping plate is arranged at the bottom of the cover body; the movable plate is arranged on the surface of the cover body; the culture dish has the beneficial effects that in actual use, the air pipe is connected with the peristaltic pump and the air filter, the peristaltic pump indirectly filters air through the air filter and then transmits the filtered air into the air pipe, the cover body covers the top of the culture dish, the high-density high-water-absorption polyester fiber plate is located on the surface of the limiting ring, and culture liquid exists at the bottom of the culture dish; air enters the culture solution through the air pipe, so that the culture solution generates bubbles.

Description

Technical Field

[0001] The utility model relates to the field of culturing sterile seedlings, in particular to a device system for culturing sterile seedlings in liquid tissue. Background Art

[0002] Since its inception, plant tissue culture technology has been widely used in crop breeding, detoxification and rapid propagation of flowers, fruit trees, vegetables, trees, and Chinese herbal medicines. It possesses advantages unmatched by other technical means and has become an indispensable technical field in modern biotechnology. To achieve efficient and low-cost factory-scale seedling production through tissue culture, many scholars at home and abroad have conducted a series of studies on improving tissue culture technology itself, such as replacing culture containers and culture substrates, rapid propagation of non-test tube seedlings, sugar-free culture, and liquid culture.

[0003] However, the existing technology directly places the plant tissue together with the solid culture medium in a culture dish. As the culture time increases, the nutrients on the surface of the solid culture medium continue to decrease, while the nutrients and trace elements in the lower part cannot be absorbed and fully utilized. Utility Model Content

[0004] The purpose of the utility model is to provide a liquid tissue culture sterile seedling device system to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a liquid tissue culture sterile seedling device system, comprising:

[0006] A culture dish, wherein a limiting ring is provided inside the culture dish;

[0007] A high-density, highly absorbent polyester fiber board, wherein the surface of the high-density, highly absorbent polyester fiber board is provided with vertical rods, the ends of the vertical rods are provided with extension plates, the top of the culture dish is provided with a cover body, the surface of the cover body is provided with an air pipe and a through groove, and the bottom of the cover body is provided with a clamping plate; and

[0008] The movable plate is arranged on the surface of the cover body.

[0009] Preferably, a breathable membrane is provided inside the through groove, a high-density and highly absorbent polyester fiber board is located on the top of the limiting ring, and the trachea passes through the inside of the high-density and highly absorbent polyester fiber board, culture fluid is placed at the bottom of the culture dish, and the end of the trachea is located in the culture fluid.

[0010] Preferably, plant tissue is placed on the top of the high-density and highly absorbent polyester fiber board, a movable groove is opened inside the cover body, a movable block is arranged in the movable groove, the end of the movable board is connected to the surface of the movable block, the movable block can move in the movable groove, and the movable block moves with the movable board.

[0011] Preferably, a connecting tube is provided inside the cover body, the connecting tube communicates with the interior of the movable groove, an annular groove is provided inside the cover body, a rubber tube is provided inside the annular groove, and the interior of the connecting tube communicates with the interior of the rubber tube.

[0012] Preferably, a clamping plate is inserted into the bottom of the cover body, one end of the clamping plate is connected to the surface of the rubber tube, and after the clamping plate moves, it and the cover body clamp the extension plate in the middle position.

[0013] Preferably, one end of the air tube located outside the culture dish is connected to an air filter and a peristaltic pump.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] During actual use of the utility model, the trachea is connected to the peristaltic pump and the air filter. The peristaltic pump indirectly filters the air through the air filter and transmits it into the trachea. The cover body covers the top of the culture dish. The high-density and highly water-absorbent polyester fiber board is located on the surface of the limiting ring. There is culture fluid at the bottom of the culture dish. Air enters the culture fluid through the trachea, causing bubbles to be generated in the culture fluid. After the bubbles burst, a small amount of culture fluid remains in the high-density and highly water-absorbent polyester fiber board. Plant tissue absorbs the culture fluid in the high-density and highly water-absorbent polyester fiber board, and the air in the trachea enters the culture fluid, causing the oxygen content in the culture fluid to increase. The increased air inside the culture dish is discharged through the breathable membrane in the through groove. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the cross-sectional structure of the utility model;

[0018] Figure 3 for Figure 2 A magnified schematic diagram of the structure in the middle.

[0019] In the figure: culture dish 1, cover 2, through groove 3, trachea 4, high-density and highly absorbent polyester fiber board 6, vertical rod 7, limiting ring 8, moving plate 9, moving groove 10, moving block 11, connecting pipe 12, extension plate 13,

[0020] Clamping plate 14 and annular groove 15. DETAILED DESCRIPTION

[0021] In order to clearly and completely describe the purpose and technical solution of the present invention and make its advantages more clearly understood, the following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only part of the embodiments of the present invention, not all of them, and are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] See also Figure 1-Figure 3 The present invention provides a technical solution: a liquid tissue culture sterile seedling device system, comprising:

[0023] A limiting ring 8 is provided inside the culture dish 1; a vertical rod 7 is provided on the surface of the high-density and highly water-absorbent polyester fiber board 6, an extension plate 13 is provided at the end of the vertical rod 7, a cover 2 is provided on the top of the culture dish 1, an air tube 4 and a through groove 3 are provided on the surface of the cover 2, a clamping plate 14 is provided at the bottom of the cover 2, a breathable membrane is provided inside the through groove 3, the high-density and highly water-absorbent polyester fiber board 6 is located at the top of the limiting ring 8, and the air tube 4 passes through the interior of the high-density and highly water-absorbent polyester fiber board 6, a culture solution is placed at the bottom of the culture dish 1, the end of the air tube 4 is located in the culture solution, and the top of the high-density and highly water-absorbent polyester fiber board 6 is placed. Plant tissue is placed therein, and a moving groove 10 is opened inside the cover 2. A moving block 11 is provided in the moving groove 10. The end of the moving plate 9 is connected to the surface of the moving block 11. The moving block 11 can move in the moving groove 10. The moving block 11 moves along with the moving plate 9. The end of the trachea 4 located outside the culture dish 1 is connected to the air filter and the peristaltic pump. The moving plate 9 is pinched in the direction of the trachea 4. The moving plate 9 drives the moving block 11 to move in the moving groove 10, so that the air in the moving groove 10 is squeezed into the rubber tube inside the annular groove 15 through the connecting tube 12. The rubber tube expands and pushes the clamping plate 14.

[0024] The movable plate 9 is arranged on the surface of the cover body 2, and a connecting pipe 12 is provided inside the cover body 2. The connecting pipe 12 is communicated with the interior of the movable groove 10. An annular groove 15 is opened inside the cover body 2, and a rubber tube is provided inside the annular groove 15. The interior of the connecting pipe 12 is communicated with the interior of the rubber tube. A clamping plate 14 is inserted into the bottom of the cover body 2, and one end of the clamping plate 14 is connected to the surface of the rubber tube. After the clamping plate 14 moves, it and the cover body 2 clamp the extension plate 13 in the middle position.

[0025] In actual use, the trachea 4 is connected to the peristaltic pump and the air filter. The peristaltic pump indirectly filters the air through the air filter and then transmits it into the trachea 4. The cover 2 covers the top of the culture dish 1. The high-density and highly absorbent polyester fiber board 6 is located on the surface of the limiting ring 8. There is culture fluid at the bottom of the culture dish 1. Air enters the culture fluid through the trachea 4, causing bubbles to form in the culture fluid. After the bubbles burst, a small amount of culture fluid remains in the high-density and highly absorbent polyester fiber board 6. The plant tissue absorbs the culture fluid in the high-density and highly absorbent polyester fiber board 6, and the air in the trachea 4 The air enters the culture medium, which increases the oxygen content in the culture medium. The increased air inside the culture dish 1 is discharged through the breathable membrane in the through groove 3. The movable plate 9 is pinched in the direction of the trachea 4. The movable plate 9 drives the movable block 11 to move in the movable groove 10, so that the air in the movable groove 10 is squeezed into the rubber tube inside the annular groove 15 through the connecting tube 12. The rubber tube expands and pushes the clamping plate 14, so that the clamping plate 14 moves. The clamping plate 14 and the cover body 2 clamp the extension plate 13, so that when the cover body 2 is taken out, the high-density and high-water-absorbent polyester fiber board 6 is taken out through the vertical rod 7.

[0026] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A liquid tissue culture sterile seedling device system, characterized by: include: A culture dish (1), wherein a limiting ring (8) is provided inside the culture dish (1); A high-density, highly water-absorbent polyester fiber board (6), a vertical rod (7) is provided on the surface of the high-density, highly water-absorbent polyester fiber board (6), an extension plate (13) is provided at the end of the vertical rod (7), a cover (2) is provided on the top of the culture dish (1), an air tube (4) and a through groove (3) are provided on the surface of the cover (2), and a clamping plate (14) is provided at the bottom of the cover (2); and The movable plate (9) is arranged on the surface of the cover body (2).

2. The liquid tissue culture sterile seedling device system according to claim 1, characterized in that: A breathable membrane is provided inside the through groove (3), a high-density, highly water-absorbent polyester fiber board (6) is located on the top of the limiting ring (8), and the trachea (4) passes through the inside of the high-density, highly water-absorbent polyester fiber board (6), a culture solution is placed at the bottom of the culture dish (1), and the end of the trachea (4) is located in the culture solution.

3. The liquid tissue culture sterile seedling device system according to claim 2, characterized in that: Plant tissue is placed on the top of the high-density and highly absorbent polyester fiber board (6), a movable groove (10) is provided inside the cover body (2), a movable block (11) is provided in the movable groove (10), the end of the movable plate (9) is connected to the surface of the movable block (11), the movable block (11) can move in the movable groove (10), and the movable block (11) moves along with the movable plate (9).

4. The liquid tissue culture sterile seedling device system according to claim 3, characterized in that: A connecting pipe (12) is provided inside the cover body (2), and the connecting pipe (12) is communicated with the inside of the movable groove (10). An annular groove (15) is provided inside the cover body (2), and a rubber tube is provided inside the annular groove (15). The inside of the connecting pipe (12) is communicated with the inside of the rubber tube.

5. The liquid tissue culture sterile seedling device system according to claim 4, characterized in that: A clamping plate (14) is inserted into the bottom of the cover body (2), one end of the clamping plate (14) is connected to the surface of the rubber tube, and after the clamping plate (14) moves, it and the cover body (2) clamp the extension plate (13) in the middle position.

6. The liquid tissue culture sterile seedling device system according to claim 5, characterized in that: One end of the air tube (4) located outside the culture dish (1) is connected to an air filter and a peristaltic pump.

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