Stem cell resuscitation device

Abstract

The utility model discloses a stem cell resuscitation device. The stem cell resuscitation device comprises a fixing structure, a driving assembly, a connecting assembly and a cryopreservation tube placing disc, the fixing structure is fixedly connected with the water bath kettle, the driving assembly is installed on the fixing structure, the driving assembly is connected with the connecting assembly, the connecting assembly is connected with the cryopreservation tube containing disc, the driving assembly drives the cryopreservation tube containing disc to rotate through the connecting assembly, and a plurality of cryopreservation tube inserting holes are formed in the cryopreservation tube containing disc; according to the utility model, the fixing structure is connected with the external water bath kettle for fixation, and the connecting assembly can be continuously rotated through the driving assembly, so that the cryopreservation tube placing disc and a plurality of cryopreservation tubes immersed in warm water on the disc are continuously rotated, the cryopreservation tubes are uniformly heated, the rewarming efficiency is improved, and the production cost is reduced. And meanwhile, the problem of arm fatigue of a closed person is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell resuscitation devices, and in particular relates to a stem cell resuscitation device. Background Art

[0002] The statements herein merely provide background art related to the present invention and do not necessarily constitute prior art.

[0003] The long-term storage of stem cells is achieved by using deep cryogenics (liquid nitrogen). They remain dormant during prolonged refrigeration, so they must be revived for subsequent use to restore their activity. Cell resuscitation involves thawing cells frozen in -196°C liquid nitrogen to 37°C. The thawing process requires constant temperature and rapid operation, allowing cells to quickly pass through the -5°C to 0°C range, where they are most vulnerable, to prevent ice crystals from re-crystallizing and causing damage to the cells, leading to cell death.

[0004] In the prior art, the commonly used cell recovery method is to place the cryopreservation tube in a water bath preheated to 37°C, and shake it quickly to quickly thaw the cell freezing solution in the cryopreservation tube. However, when manually recovering cells, in order to ensure that the cell freezing solution is fully and quickly melted in the water bath, it is impossible to complete the recovery of multiple cells at a time, which has low work efficiency and will cause arm fatigue to the relevant personnel. In addition, due to individual operational differences, such as inconsistent cell shaking frequency and amplitude, it is impossible to guarantee consistent recovery time for each tube of cells, which may affect the viability and quality of the cells and lead to differences between recovered cells. In addition, during recovery, the cell cryopreservation tube needs to be completely immersed in the water bath, which poses a certain risk of contamination. Therefore, this method is not suitable for large-scale stem cell recovery. Utility Model Content

[0005] The purpose of the utility model is to provide a stem cell resuscitation device that can resuscitate large quantities of stem cells while ensuring the viability of the stem cells and avoiding the problem of arm fatigue of relevant personnel; in addition, the device is convenient to store, easy to use, and occupies a small space.

[0006] In order to achieve the above purpose, the present invention is implemented through the following technical solutions:

[0007] In the first aspect, an embodiment of the present invention provides a stem cell resuscitation device, comprising a fixed structure, a drive assembly, a connecting assembly and a cryotube placement tray; the fixed structure is fixedly connected to a water bath, the drive assembly is mounted on the fixed structure, the drive assembly is connected to the connecting assembly, the connecting assembly is connected to the cryotube placement tray, the drive assembly drives the cryotube placement tray to rotate through the connecting assembly, and a plurality of cryotube insertion holes are provided on the cryotube placement tray.

[0008] As a further technical solution, the fixing structure includes a circular main body bracket and a plurality of fixing brackets, the plurality of fixing brackets are evenly connected to the side surfaces of the main body bracket, and the fixing brackets are telescopic rods.

[0009] As a further technical solution, a magnet is provided at one end of the fixing bracket away from the main bracket, and the magnet is attracted to the water bath by magnetic force.

[0010] As a further technical solution, the driving assembly includes a driving motor, the output shaft of the driving motor is connected to the first gear, and the driving motor is fixedly mounted on the inner wall surface of the main body bracket.

[0011] As a further technical solution, the connecting assembly includes a connecting rod, one end of which is rotatably connected to the center position of the main bracket through a bearing, and the other end of the connecting rod is connected to a fixed rod, and the end of the fixed rod is fixedly connected to the center of the cryotube placement tray.

[0012] As a further technical solution, a second gear is fixedly installed at a position of the connecting rod close to the main body bracket, and the second gear is engaged with the first gear.

[0013] As a further technical solution, two cryopreservation tube bag fixing clamps are fixedly connected to the connecting rod, and the cryopreservation tube bag fixing clamps seal the opening of the cryopreservation tube bag.

[0014] As a further technical solution, the connecting rod and the fixing rod are both retractable rods.

[0015] As a further technical solution, the diameter of the cryotube placement tray matches the inner diameter of the circular main body bracket, and when the connecting rod and the fixing rod are both retracted, the cryotube placement tray can be placed in the circular main body bracket.

[0016] As a further technical solution, the diameter of the cryotube insertion hole matches the diameter of the cryotube.

[0017] The beneficial effects of the above embodiments of the present invention are as follows:

[0018] The stem cell resuscitation device provided by the present invention is fixed to an external water bath via a fixed structure, and the connecting assembly can be continuously rotated by a driving assembly, thereby causing the cryotube placement tray and multiple cryotubes immersed in warm water on the tray to continuously rotate, thereby evenly heating the cryotubes, improving the rewarming efficiency, and avoiding the problem of arm fatigue of the personnel.

[0019] The stem cell resuscitation device provided by this utility model is also convenient for storage. With simple adjustments, the device can be stored in the side wall of a water bath. Specifically, the connecting rod and the fixing rod are adjusted to their minimum length, and the cryotube tray is placed in the bottom surface of the main bracket. Finally, the fixing bracket is adjusted to its minimum length and fixed to the side wall of the water bath. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention.

[0021] Figure 1 This is a schematic diagram of the overall structure of the stem cell resuscitation device of the present invention;

[0022] Figure 2 This is a schematic diagram of the stem cell resuscitation device of the present invention when in use;

[0023] Figure 3 This is a schematic diagram of the stem cell resuscitation device of the present invention after being stored;

[0024] Figure 4 This is a schematic diagram of the stem cell resuscitation device of the present invention being installed on a water bath after being stored.

[0025] The diagram is for illustrative purposes only;

[0026] Among them, 1. fixed structure; 11. main bracket; 12. fixed bracket; 13. magnet; 2. drive assembly; 21. drive motor; 22. first gear; 23. second gear; 3. connecting assembly; 31. connecting rod; 32. cryopreservation tube bag fixing clamp; 33. fixing rod; 4. cryopreservation tube placement tray; 41. cryopreservation tube jack. DETAILED DESCRIPTION

[0027] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used in the present invention have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0028] Example 1

[0029] In a typical embodiment of the present invention, Figure 1 and Figure 2As shown, a stem cell resuscitation device is provided, including a fixed structure 1, a driving component 2, a connecting component 3 and a cryotube placement tray 4; the fixed structure 1 is fixedly connected to a water bath 5, the driving component 2 is installed on the fixed structure 1, the driving component 2 is connected to the connecting component 3, the connecting component 3 is connected to the cryotube placement tray 4, the driving component 2 drives the cryotube placement tray 4 to rotate through the connecting component 3, and a plurality of cryotube jacks 41 are provided on the cryotube placement tray 4.

[0030] In this embodiment, the fixed structure 1 includes a main frame 11 and multiple fixed frames 12. The main frame 11 is a circular, downward-opening cylindrical structure. The fixed frames 12 are telescopic rods, and the multiple fixed frames 12 are evenly connected to the sides of the main frame 11. In a specific implementation of this embodiment, four fixed frames 12 are provided, connected to the main frame in a "cross" shape. The fixed frames 12 can be extended within a certain range to accommodate different models of water baths.

[0031] Furthermore, a magnet 13 is provided at one end of the fixing bracket 12 away from the main bracket 11. The magnet 13 and the water bath 5 are attracted to each other by magnetic force, thereby fixing the entire device to the water bath 5. In a specific implementation of this embodiment, the magnet 13 has an arc-shaped structure, and the curvature of the inner arc of the magnet matches the curvature of the outer wall of the fixing bracket 12. When the fixing bracket 12 is retracted, the magnet 13 can be attached to the main bracket 11, thereby reducing the space occupied by the entire device.

[0032] In this embodiment, the driving assembly 2 includes a driving motor 21, the output shaft of the driving motor 21 is connected to the first gear 22, the driving motor 21 is fixedly mounted on the inner wall surface of the main bracket 11, and the driving motor is powered by a built-in power supply.

[0033] In this embodiment, the connecting assembly 3 includes a connecting rod 31, one end of which is rotatably connected to the center position of the main bracket 11 through a bearing, and the other end of the connecting rod 31 is connected to the fixing rod 33. The end of the fixing rod 33 is fixedly connected to the center of the cryotube placement tray 4. The diameter of the cryotube socket 41 on the cryotube placement tray 4 matches the diameter of the cryotube so that the cryotube can be inserted into the cryotube socket 41 for fixing.

[0034] Furthermore, a second gear 23 is fixedly installed on the connecting rod 31 near the main frame 11, and the second gear 23 is engaged with the first gear 22, and the power of the drive motor 21 is transmitted to the connecting component 3 and the cryotube placement tray 4 through the first gear 22 and the second gear 22.

[0035] Furthermore, two cryotube bag clamps 32 are fixedly connected to the connecting rod to seal the opening of the cryotube bag. The cryotube bag clamps 32 are composed of two horizontally placed magnets, the outer surfaces of which are encapsulated in plastic. The cryotube bag is secured with a PE glove. During use, the cryotube is placed between the fingers of the PE glove to prevent water from entering the cryotube and contaminating the cells. The top of the glove is then clamped and secured with the two magnets of the cryotube bag clamps 32.

[0036] In this embodiment, the connecting rod 31 and the fixing rod 33 are both retractable rods, and the diameter of the cryotube placement tray 4 is smaller than the inner diameter of the circular main body bracket 11. When the connecting rod 31 and the fixing rod 33 are retracted, the cryotube placement tray 4 can be placed in the circular main body bracket 11, thereby achieving the storage of the entire device. Figure 3 and Figure 4 As shown, the stored device can be fixed on the side of the water bath.

[0037] The method of using the stem cell resuscitation device provided in this embodiment is as follows:

[0038] Stretch the fixing bracket 12 so that the magnet 13 at the end of the fixing bracket 12 can be adsorbed on the top of the water bath 5, place the cryotube in the finger of the PE glove, and insert the cryotube covered with the PE glove into the cryotube insertion hole 41 of the cryotube placement tray 4, then clamp the top of the glove with the two magnets of the cryotube bag fixing clamp 32, and stretch the connecting rod 31 at the same time to ensure that the cryotube placement tray is close to the water bath liquid surface, that is, by placing multiple cryotubes on the placement tray into the water bath at the same time, and it is necessary to keep the solution in the cryotube completely below the water bath liquid surface.

[0039] The drive motor 21 is energized, and the output shaft of the drive motor 21 drives the first gear 22 to rotate. Since the first gear 22 is engaged with the second gear 23 and the second gear is fixedly connected to the connecting rod 31, the first gear 22 drives the connecting assembly 3 and the cryotube placement tray 4 to rotate, so that the multiple cryotubes immersed in warm water on the cryotube placement tray are continuously rotated, so that the cryotubes are heated evenly, the rewarming efficiency is improved, and the problem of arm fatigue of the personnel is avoided.

[0040] After use, the fixing bracket 12 , the connecting rod 31 and the fixing rod 33 can be retracted to complete the storage of the device, and the device can be adsorbed and fixed on the side wall of the water bath 5 by the magnet 13 .

[0041] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A stem cell resuscitation device, characterized in that: It includes a fixed structure, a driving assembly, a connecting assembly and a freezing tube placement tray; the fixed structure is fixedly connected to the water bath, the driving assembly is installed on the fixed structure, the driving assembly is connected to the connecting assembly, the connecting assembly is connected to the freezing tube placement tray, the driving assembly drives the freezing tube placement tray to rotate through the connecting assembly, and a plurality of freezing tube jacks are provided on the freezing tube placement tray.

2. The stem cell resuscitation device according to claim 1, wherein: The fixing structure includes a circular main body support and a plurality of fixing supports, wherein the plurality of fixing supports are evenly connected to the side surfaces of the main body support, and the fixing supports are retractable rods.

3. The stem cell resuscitation device according to claim 2, wherein: A magnet is provided at one end of the fixing bracket away from the main bracket, and the magnet is attracted to the water bath pot by magnetic force.

4. The stem cell resuscitation device according to claim 2, wherein: The driving assembly includes a driving motor, an output shaft of the driving motor is connected to the first gear, and the driving motor is fixedly mounted on the inner wall surface of the main body bracket.

5. The stem cell resuscitation device according to claim 4, wherein: The connecting assembly includes a connecting rod, one end of which is rotatably connected to the center position of the main support through a bearing, and the other end of the connecting rod is connected to a fixed rod, and the end of the fixed rod is fixedly connected to the center of the freezing tube placement tray.

6. The stem cell resuscitation device according to claim 5, wherein: A second gear is fixedly mounted on the connecting rod near the main frame, and the second gear is meshed with the first gear.

7. The stem cell resuscitation device according to claim 5, wherein: Two cryopreservation tube bag fixing clamps are fixedly connected to the connecting rod, and the cryopreservation tube bag fixing clamps seal the opening of the cryopreservation tube bag.

8. The stem cell resuscitation device according to claim 5, wherein: The connecting rod and the fixing rod are both retractable rods.

9. The stem cell resuscitation device according to claim 8, wherein: The diameter of the freezing tube placement tray matches the inner diameter of the circular main body bracket. When the connecting rod and the fixing rod are both retracted, the freezing tube placement tray can be placed in the circular main body bracket.

10. The stem cell resuscitation device according to claim 1, wherein: The diameter of the freezing tube insertion hole matches the diameter of the freezing tube.

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