Waste liquid dumping device

Abstract

The utility model relates to the technical field of cell sheet preparation, in particular to a waste liquid dumping device applied to a full-automatic liquid-based cell sheet preparation machine, which comprises a sample bottle push rod and a sample bottle bearing seat, the sample bottle bearing seat is used for bearing a sample bottle and can swing around a shaft; the sample bottle push rod is arranged on one side of the sample bottle bearing seat and can push the sample bottle bearing seat to swing; the sample bottle push rod comprises a horizontal push rod and a vertical push rod; the horizontal push rod is positioned above the vertical push rod; the horizontal push rod is pushed to contact with the upper part of the side wall of the sample bottle, so that the sample bottle drives the sample bottle bearing seat to swing and incline around the shaft; after being pushed, the vertical push rod is in contact with the lower part of the side wall of the inclined sample bottle bearing seat, so that the sample bottle bearing seat drives the sample bottle to continuously swing and incline around the shaft. The waste liquid dumping device adopts a push rod to push a sample bottle to dump and discharge centrifuged sample supernate, a liquid path and gas path system does not need to be arranged, the mechanical structure is simplified, the running reliability of the device is improved, and the failure rate is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell preparation, in particular to a waste liquid dumping device applied to a full-automatic liquid-based cell preparation machine. Background Art

[0002] The existing liquid-based cell preparation technology using the natural sedimentation method includes a step of centrifuging cells to enrich the cells, and the waste liquid generated by the centrifugation is generally discharged by suction.

[0003] For example, patent CN211784689U provides a fully automatic pathology slide staining machine, which includes: a main body, a pump group, a centrifuge, a sample slot, a staining slot, a first nozzle placement slot, a second nozzle placement slot, a nozzle liquid collection arm, a dye adding and extracting arm and an arm horizontal movement module, making cell preparation and staining operations more convenient and quick, providing a good hardware equipment foundation for improving work efficiency and accuracy, so as to make the analysis of complex specimens more effective.

[0004] The problem is that two robotic arms are required: one for aspirating and discharging the centrifugal supernatant after cell enrichment, and the other for sample transfer, reagent injection, and waste liquid removal. Because the aspiration method requires coordinated gas and liquid pathways within the mechanical structure, the system is complex, prone to failure, and difficult to maintain.

[0005] Therefore, there is an urgent need for a waste liquid dumping device with simple structure to solve the problems of complex machine structure and easy failure. Summary of the Invention

[0006] The utility model provides a full-automatic liquid-based cell slice making machine to solve the problems of complex machine structure and easy failure in related technologies.

[0007] To achieve the above-mentioned purpose, the utility model provides a waste liquid dumping device, including a sample bottle push rod and a sample bottle support seat; the sample bottle support seat is used to support the sample bottle, and shafts are provided on both sides of the upper part of the sample bottle support seat and can swing around the shaft; the sample bottle push rod is arranged on one side of the sample bottle support seat, and can push the sample bottle support seat to swing; the sample bottle push rod includes a horizontal push rod and a vertical push rod, and the horizontal push rod is located above the vertical push rod; after the horizontal push rod is pushed, it contacts the upper part of the side wall of the sample bottle, and can push the sample bottle in the horizontal direction, so that the sample bottle drives the sample bottle support seat to swing and tilt around the axis; after the vertical push rod is pushed, it contacts the lower part of the side wall of the inclined sample bottle support seat, and can push the sample bottle support seat in the vertical direction, so that the sample bottle support seat drives the sample bottle to continue to swing and tilt around the axis.

[0008] Furthermore, the horizontal push rod is located on the upper part of the sample bottle supporting seat, and includes a first vertical arm, a first horizontal arm and a second driving device, the first vertical arm and the first horizontal arm are connected in a T-shape, the first horizontal arm is perpendicular to the radius of the centrifugal turntable, and the second driving device is connected to the first vertical arm;

[0009] The vertical push rod is located below the sample bottle supporting seat, and includes a second vertical arm, a second horizontal arm and a third driving device. The second vertical arm and the second horizontal arm are connected in a T shape. The second horizontal arm is perpendicular to the radius of the centrifugal turntable. The third driving device is connected to the second vertical arm.

[0010] Furthermore, a fixing block is provided in the middle of the second cross arm, and the size of the fixing block is adapted to the distance between the two sample bottle supporting seats.

[0011] Furthermore, it also includes a waste liquid collection device, which includes a waste liquid receiving tray, a second waste liquid pipeline and a waste liquid bottle;

[0012] The waste liquid receiving tray is arranged below the centrifugal turntable and is in the shape of a ring. The waste liquid receiving tray includes a tray bottom, an inner tray wall and an outer tray wall. The inner tray wall is located in a circle around the inner periphery of the tray bottom, and the outer tray wall is located in a circle around the outer periphery of the tray bottom. The inner tray wall and the outer tray wall protrude from the tray bottom. The tray bottom is provided with a waste liquid port that passes through from top to bottom. The lower end of the waste liquid port is connected to the waste liquid bottle through a second waste liquid pipeline. A third valve is provided on the pipeline, and the switch of the third valve is controlled by a controller.

[0013] Beneficial effects of the utility model:

[0014] The waste liquid dumping device uses a push rod to push the sample bottle to dump and discharge the supernatant of the sample after centrifugation. It does not require the setting of liquid and gas systems, simplifies the mechanical structure, improves the reliability of the device operation, and reduces the failure rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0016] Figure 1 This is a schematic diagram of the fully automatic liquid-based cell slide making machine of the utility model;

[0017] Figure 2 This is a schematic diagram of the fully automatic liquid-based cell slide making machine of the utility model;

[0018] Figure 3 This is a schematic diagram of the fully automatic liquid-based cell slide making machine of the utility model;

[0019] Figure 4This is a schematic diagram of the fully automatic liquid-based cell slide making machine of the utility model;

[0020] Figure 5 This is a schematic diagram of the transport module of the utility model fully automatic liquid-based cell slide making machine;

[0021] Figure 6 This is a schematic diagram of the liquid aspiration and injection mechanism of the fully automatic liquid-based cell slide making machine of the present utility model;

[0022] Figure 7 This is a schematic diagram of the aspiration and injection components of the fully automatic liquid-based cell slide making machine of the present utility model;

[0023] Figure 8 This is a schematic diagram of the waste liquid collection device and waste liquid dumping device of the fully automatic liquid-based cell slide making machine of the present utility model;

[0024] Figure 9 This is a schematic diagram of the waste liquid collection device and waste liquid dumping device of the fully automatic liquid-based cell slide making machine of the present utility model;

[0025] Figure 10 This is a schematic diagram of the waste liquid collection device and waste liquid dumping device of the fully automatic liquid-based cell slide making machine of the present utility model;

[0026] Figure 11 This is a schematic diagram of the balancing block of the fully automatic liquid-based cell slide making machine of the present utility model;

[0027] Figure 12 This is a schematic diagram of the slide clamp assembly of the fully automatic liquid-based cell slide making machine of the present invention.

[0028] in:

[0029] 1. Fully automatic liquid-based cell slicer; 2. Transport module; 3. Cell enrichment module; 4. Pipette tip module; 5. Slice preparation module; 6. Robotic arm; 7. Aspiration and injection mechanism; 8. Aspiration and injection assembly; 9. Cannula; 10. Aspiration needle; 11. Injection needle; 12. Centrifugal turntable; 13. First drive unit; 14. Waste liquid dumping device; 15. Self-leveling assembly; 16. Sample bottle holder; 17. Shaft; 18. Sample bottle; 19. Sample bottle push rod; 20. Measurement unit; 21. Balancing block; 22. Transfer suction cup; 23. X-arm; 24. Y-arm; 25. Z-arm; 26. Upper end of the aspiration needle; 27. First waste liquid pipeline; 28. Waste liquid bottle; 29. ​​First pump; 30. First valve; 31. Controller; 32. Upper end of the injection needle; 33. Reagent pipeline; 34. Reagent bottle; 35. Second pump; 36. Second valve; 37. Lower end of the aspiration needle; 38. Horizontal push rod; 39. Vertical push rod; 40. First vertical arm; 4 1. First horizontal arm; 42. Second driving device; 43. Second vertical arm; 44. Second horizontal arm; 45. Third driving device; 46. Fixed block; 47. Waste liquid collection device; 48. Waste liquid receiving tray; 49. Second waste liquid pipeline; 50. Tray bottom; 51. Inner tray wall; 52. Outer tray wall; 53. Recessed space; 54. Waste liquid outlet; 55. Third valve; 56. Anti-slip pad; 57. Positioning sensor; 58. Bottle detection sensor; 59. Component 1; 60. Component Item 2; 61. Pipette tip holder; 62. Photoelectric sensor; 63. Pipette tip position; 64. Workbench; 65. Slide clamp assembly; 66. Work station; 67. Slide holder; 68. Sedimentation chamber; 69. Slide; 70. Sealing rubber ring; 71. Clip; 72. Solid waste collection module; 73. Tube removal assembly; 74. Waste collection box; 75. Support plate; 76. Gun removal plate; 77. Avoidance part; 78. Disposable pipette tip; 79. Socket; 80. Rinse tube. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] like Figure 1-4As shown, the utility model provides a fully automatic liquid-based cell preparation machine 1, comprising: a transport module 2, a cell enrichment module 3, a pipette tip module 4 and a preparation module 5, the cell enrichment module 3, the pipette tip module 4 and the preparation module 5 are arranged in sequence, and the transport module 2 is arranged above the cell enrichment module 3, the pipette tip module 4 and the preparation module 5. The transport module 2 includes a robotic arm 6 and a liquid suction and injection mechanism 7, and the liquid suction and injection mechanism 7 is fixedly mounted on the robotic arm 6. The cell enrichment module 3 includes: a centrifugal turntable 12, a waste liquid dumping device 14, a self-leveling component 15 and a first drive device 13. Sample bottle holders 16 are evenly arranged on the periphery of the centrifugal turntable 12 for carrying sample bottles 18. Both sides of the upper part of the sample bottle holder 16 are connected to the axis of the centrifugal turntable 12, and the sample bottle holder 16 can swing around the axis 17. The waste liquid dumping device 14 is composed of a sample bottle push rod 19 . The sample bottle push rod 19 can push the sample bottle supporting seat 16 to make the sample bottle supporting seat 16 swing along the axis 17 .

[0032] The robotic arm 6 on the transport module 2 is used to drive the aspiration and injection mechanism 7 to move within the fully automatic liquid-based cell slide preparation machine 1, completing each step of the slide preparation process between different modules, including mixing the sample, transferring the sample, adding reagents, and aspirating and draining waste liquid. The centrifugal turntable 12 on the cell enrichment module 3 is used to enrich the cells in the sample, and the waste liquid dumping device 14 is used to discharge the supernatant after centrifugation, so that the centrifugal pellet of diagnostically significant cells remains at the bottom of the sample bottle. The slide preparation module 5 is used for sedimentation preparation and staining of cell samples, and the pipette tip module 4 is used to load disposable pipette tips 78.

[0033] Specifically, first, the cell enrichment module 3 deposits the cell sample stored in the sample bottle 18 at the bottom tip of the sample bottle 18 through centrifugation, that is, the centrifugal turntable 12 rotates, and the sample bottle support 16 swings around the rotating shaft 17 to a horizontal state under the action of centrifugal force, the centrifugal radius increases, and the centrifugal effect is good. After centrifugation, the sediment gathers at the pointed bottom of the sample bottle 18, which is convenient for subsequent mixing.

[0034] Then, waste liquid dumping device 14 pushes sample bottle 18, causing it to swing around axis 17 within sample bottle holder 16 until it reaches a horizontal position, or even until the mouth of sample bottle 18 is lower than the bottom of the bottle. The supernatant from the centrifugation in sample bottle 18 automatically flows out under the action of gravity. Subsequently, waste liquid dumping device 14 resets, and sample bottle 18 automatically returns to its vertical position. This operation leaves only cells of diagnostic significance in sample bottle 18, achieving the purpose of cell enrichment.

[0035] Then, the aspiration and injection mechanism 7 on the transport module 2 picks up the disposable pipette tip 78 in the pipette tip module 4 by plugging it in, injects liquid into the enriched cells, resuspends the cells in the liquid, and mixes them. The disposable pipette tip 78 is then used to transfer the resuspended cells from the enrichment module 3 to the smearing module 5.

[0036] Finally, the liquid injection mechanism 7 on the transport module 2 injects reagents and drains waste liquid into the slide making module 5, assisting the slide making module 5 in completing slide making and staining. It is understandable that slide making usually refers to sedimentation slide making, and staining usually refers to cell staining.

[0037] Through the above embodiment, the cooperation between the centrifugal turntable 12 and the waste liquid dumping device 14 of the cell enrichment module 3 can automatically complete the operations of centrifugation and dumping the supernatant without manual intervention to obtain enriched cells.

[0038] Through the above embodiment, the transport module 2 cooperates with the disposable pipette tip 78 to complete the resuspension, mixing and transfer of the enriched cells, and injects different reagents and absorbs and discharges waste liquid in each step of the preparation process through the aspiration and injection component 8 of the transport module 2 to complete the preparation and staining.

[0039] The advantages of the above embodiment are that it eliminates the need for a robotic arm for aspirating the centrifugal supernatant and instead employs a simple waste liquid dumping device 14 for naturally dumping the supernatant, leaving only one robotic arm 6 for resuspending, mixing, and transferring cells. This makes the fully automatic liquid-based cell slide preparation machine of the present invention compact and simple, making it easy to maintain. Furthermore, the independent waste liquid dumping device 14 frees the transport module 2 from the task of discharging waste liquid, allowing waste liquid dumping and sample transfer to proceed simultaneously, thereby improving work efficiency.

[0040] Further, such as Figure 8-11 As shown, the self-balancing assembly 15 includes a detection unit 20, a plurality of balancing blocks 21, and a transfer suction cup 22. The detection unit 20 detects whether the sample bottles in the centrifugal turntable 12 are balanced and records the sample bottle holders 16 that need to be balanced. The transfer suction cup 22 is fixed to the transfer module 2. The robot arm 6 drives the transfer suction cup 22 to absorb the balancing blocks 21 and place them in the sample bottle holders 16 that need to be balanced.

[0041] The self-balancing component 15 is used for automatic balancing before centrifugation, which reduces labor and improves the degree of automation.

[0042] The specific process of automatic balancing is as follows: the sample bottle 18 storing the cell sample is placed in the sample bottle holder 16 of the cell enrichment module 3, the detection unit 20 of the self-balancing component 15 detects whether the sample bottle 18 is balanced, and records the sample bottle holder 16 that needs to be balanced, and transmits the information to the controller 31 (not shown in the figure); the controller 31 controls the robotic arm 6 to drive the transfer suction cup 22 to absorb the balancing block 21, and places it in the sample bottle holder 16 that needs to be balanced, thereby realizing the automatic balancing function.

[0043] Through the above embodiment, the self-balancing component cooperates with the transport module 2 to automatically complete the balancing work of the cell enrichment module before centrifugation to enrich cells, without manual participation, and with a high degree of automation.

[0044] In summary, through the above embodiment, the cell enrichment module 3, the transport module 2 and the preparation module 5 work together to complete the entire process of preparing glass slide specimens from samples collected from the laboratory with almost no human intervention. The degree of automation is high, and the fully automatic liquid-based cell preparation machine has a compact and simple structure and is easy to maintain.

[0045] Further, such as Figure 5-7 As shown, the robotic arm 6 includes: an X-arm 23, a Y-arm 24 and a Z-arm 25. The X-arm 23 is movably mounted on one side of the film-making module, the Y-arm 24 is movably mounted on the X-arm 23, the Z-arm 25 is movably mounted on the Y-arm 24, and the liquid suction and injection mechanism 7 is fixedly set on the Z-arm 25.

[0046] Specifically, the fully automatic liquid-based cell preparation machine is provided with a vertical plate on one side of the preparation module, and the vertical plate can be located on the side of the preparation module, and at this time the vertical plate is parallel to the line connecting the cell enrichment module 3, the pipette tip module 4 and the preparation module 5; the vertical plate can also be arranged in sequence with the cell enrichment module 3, the pipette tip module 4 and the preparation module 5, and at this time, the vertical plate is perpendicular to the line connecting the cell enrichment module 3, the pipette tip module 4 and the preparation module 5.

[0047] A first motor, a first guide rail, and a first belt are fixedly mounted on the vertical plate. The first motor is fixedly mounted at one end of the vertical plate. The first belt is horizontally arranged, with one end passing over a first driving pulley on the output shaft of the first motor and the other end passing over a first driven pulley fixed to the vertical plate. The first motor drives the first belt in horizontal reciprocating motion. The first guide rail is fixedly mounted on the vertical plate, parallel to and adjacent to the first belt.

[0048] The X-arm 23 is mounted on the first belt and the first guide rail.

[0049] The X-arm 23 includes an X-slider (not shown in the figure) and an X-support member (not shown in the figure).

[0050] One end of the X slider is fixedly mounted on the first belt, and the other end is movably mounted on the first guide rail. The X slider can move back and forth horizontally (in the X direction) along the first guide rail under the drive of the first belt. The X support member is fixedly connected to the X slider.

[0051] The first motor drives the first belt to move back and forth horizontally. The moving first belt drives the X slider to move back and forth horizontally along the first guide rail. The moving X slider drives the X support to move back and forth horizontally, thereby realizing the movement of the X arm in the X direction.

[0052] A second motor, a second guide rail, and a second belt are also fixedly mounted on the X-support. The second motor is fixed to one end of the X-support, and the second belt is arranged horizontally and perpendicular to the first belt. One end of the second belt passes over a second driving pulley on the output shaft of the second motor, and the other end passes over a second driven pulley fixed to the X-support. The second motor drives the second belt to reciprocate horizontally. The second guide rail is fixed to the X-support, parallel to and adjacent to the second belt.

[0053] The Y-arm 24 is mounted on the second belt and the second guide rail.

[0054] The Y arm 24 includes a Y slider (not shown in the figure) and a Y support member (not shown in the figure).

[0055] One end of the Y slider is fixedly mounted on the second belt, and the other end is movably mounted on the second guide rail. The Y slider can move back and forth horizontally (in the Y direction) along the second guide rail driven by the second belt. The Y support member is fixedly connected to the Y slider.

[0056] The second motor drives the second belt to move back and forth horizontally, the moving second belt drives the Y slider to move back and forth horizontally along the second guide rail, and the moving Y slider drives the Y support to move back and forth horizontally, thereby realizing the movement of the Y arm in the Y direction.

[0057] Since the Y arm 24 is movably mounted on the X arm 23 , the Y arm can move in the X direction driven by the X arm, that is, the Y arm can move in both the X and Y directions.

[0058] A third motor, a third guide rail, and a third belt are also fixedly mounted on the Y support. The third motor is fixed at one end of the Y support. The third belt is arranged horizontally and longitudinally, perpendicular to the second belt. One end of the third belt passes over a third driving pulley on the output shaft of the third motor, and the other end passes over a third driven pulley fixed to the Y support. The third motor drives the third belt in reciprocating horizontal motion. The third guide rail is fixed to the Y support, parallel to and adjacent to the third belt.

[0059] The Z arm 25 is mounted on the third belt and the third guide rail.

[0060] The Z arm 25 includes a Z slider (not shown in the figure) and a Z support member (not shown in the figure).

[0061] One end of the Z slider is fixedly mounted on the third belt, and the other end is movably mounted on the third guide rail. The Z slider can move back and forth longitudinally (in the Z direction) along the third guide rail driven by the third belt. The Z support member is fixedly connected to the Z slider.

[0062] The third motor drives the third belt to move back and forth horizontally. The moving third belt drives the Z slider to move back and forth horizontally along the third guide rail. The moving Z slider drives the Z support to move back and forth horizontally, thereby realizing the movement of the Z arm in the Z direction.

[0063] Since the Z arm 24 is movably mounted on the Y arm 23 , the Z arm can move in the X and Y directions driven by the Y arm, that is, the Z arm can move in the X, Y and Z directions.

[0064] The liquid suction and injection mechanism 7 is fixedly mounted on the Z support member and can move in the X, Y and Z directions driven by the Z arm.

[0065] That is to say, through the arrangement of the X-arm 23, the Y-arm 24 and the Z-arm 25, the aspiration and injection mechanism 7 can move freely in the working space, so as to grab the disposable pipette tip 78 at any position, transfer the samples in the sample bottles at different positions to the slide clamps at the corresponding positions, and perform aspiration and injection operations on the slide clamps at any position.

[0066] That is, the fully automatic liquid-based cell slide making machine of the present invention can complete sample transfer and liquid aspiration and injection operations by only one mechanical arm 6, thereby simplifying the mechanical structure.

[0067] Further, such as Figure 5-7 As shown, the aspiration and injection mechanism 7 includes several aspiration and injection components 8, and the aspiration and injection components 8 include a sleeve 9 and a suction needle 10 and an injection needle 11 inside the sleeve 9, and the suction needle 10 passes through the sleeve 9 and extends out.

[0068] First, the aspiration needle 10 is used to extract the waste liquid generated in each step of the film-making process from the film-making module 5, and the injection needle 11 is used to add different reagents required for each step of the film-making process into the film-making module 5 to assist in film-making.

[0069] Specifically, the aspiration needle 10 and the injection needle 11 are both capillary steel tubes with a diameter of 1-5 mm, which are easily bent and deformed, affecting aspiration and injection. The aspiration needle 10 and the injection needle 11 are integrated inside the sleeve 9. The sleeve 9 has a larger diameter and can better resist external pressure and protect the aspiration needle 10 and the injection needle 11.

[0070] Injection needle 11 is used for injection. To avoid cross-contamination between samples, it must be kept clean. Therefore, injection needle 11 is entirely housed within cannula 9. This arrangement does not affect the injection function, but also allows cannula 9 to protect injection needle 11 from deformation to the greatest extent possible. It also prevents contamination of the sample by contact with the injection needle 11 when the suction and injection mechanism 7 is inserted into various sample containers.

[0071] In order to ensure that the aspiration needle 10 successfully aspirates waste liquid while avoiding cross contamination of the injection needle 11, in addition to taking the above-mentioned measure of placing the injection needle as a whole in the cannula 9, this embodiment sets the aspiration needle 10 to pass through the cannula 9 and extend out. In this way, the aspiration needle 10 can smoothly aspirate waste liquid, and when aspirating waste liquid, only the aspiration needle 10 extending out of the cannula 9 contacts the waste liquid, while the cannula 9 and the injection needle 11 inside the cannula 9 do not contact the waste liquid, thereby avoiding cross contamination.

[0072] Furthermore, the injection needle 11 can be used to clean the aspiration needle 10 while injecting liquid. Specifically, the aspiration needle 10 and the injection needle 11 are both disposed within the cannula, and the aspiration needle 10 and the injection needle 11 are tightly fitted together, with the liquid outlet of the injection needle 11 in contact with the outer wall of the aspiration needle. When the injection needle 11 injects liquid, the reagent flows out of the liquid outlet of the injection needle 11 and flows down along the outer wall of the aspiration needle 10. The clean reagent flushes the aspiration needle 10, thereby cleaning the aspiration needle 10.

[0073] Next, the aspiration and injection mechanism 7 picks up the disposable pipette tip 78 by inserting the sleeve 9 , and resuspends and mixes the cell sample by using the disposable pipette tip 78 , and then transfers the cell sample to the slicing module 5 .

[0074] Specifically, the cannula 9, driven by the robotic arm 6, moves to the pipette tip module 4, moves downward, and inserts the cannula 9 into the disposable pipette tip 78. The disposable pipette tip 78 is picked up and returned to the cell enrichment module 3 to inject liquid into the centrifuged cells. The aspiration and injection mechanism 7 generates continuous negative and positive pressure to draw the cell sample into and out of the disposable pipette tip 78, thereby resuspending and mixing the cell sample. Finally, the cell sample is drawn into the disposable pipette tip 78, and, driven by the robotic arm 6, moves to the slicing module 5, injecting the cell sample into the slicing module 5, completing the transfer of the cell sample from the cell enrichment module 3 to the slicing module 5.

[0075] Further, such as Figure 6As shown, the upper end 26 of the liquid-pipetting needle 10 is connected to the waste liquid bottle 28 through the first waste liquid pipeline 27, and the first waste liquid pipeline 27 is provided with a first pump 29 and a first valve 30, and the lower end of the liquid-pipetting needle is bent. The upper end 32 of the liquid-injection needle 11 is connected to the reagent bottle 34 through the reagent pipeline 33, and the reagent pipeline 33 is provided with a second pump 35 and a second valve 36. A controller 31 is also included, and the controller 31 is electrically connected to the first pump 29 and the first valve 30 and the second pump 35 and the second valve 36. The controller 31 controls the switches of the first pump 29 and the first valve 30 and the second pump 35 and the second valve 36.

[0076] Specifically, the controller 31 controls the opening and closing of the second pump 35 and the second valve 36 on the reagent pipeline 33, so that the corresponding reagent is injected into the sample bottle 18 of the cell enrichment module 3 or the area of ​​the slab preparation module 5 through the injection needle 11 to complete the corresponding slab preparation step.

[0077] The controller 31 controls the opening and closing of the first pump 29 and the first valve 30 on the first waste liquid pipeline 27 so that the aspiration needle 10 aspirates the waste liquid in the film-making module 5 for subsequent film-making steps.

[0078] As a possible implementation, Figure 7 As shown, there are 1 to 5 injection needles 11, which are used to inject different reagents respectively.

[0079] Specifically, cell preparation and staining often require 1-5 reagents with different physical and chemical properties, which need to be transported through different pipelines to prevent reagent mixing, affecting the physical and chemical properties of the reagents, causing preparation failure, and affecting the staining effect.

[0080] In this embodiment, 1 to 5 injection needles 11 and the aspiration needle 10 are integrated into one cannula, which meets the need for injecting a variety of different reagents while maintaining a compact structure.

[0081] As a possible implementation, Figure 7 As shown, the lower end 37 of the pipette needle is bent.

[0082] Specifically, when the aspiration needle 10 aspirates, the curved lower end can be as close to the cell layer on the glass slide 69 as possible to aspirate as much waste liquid above the cell layer as possible, while preventing the sharp lower end 37 from directly contacting the cell layer and damaging the cell layer.

[0083] At the same time, when the injection needle 11 injects liquid, the liquid flows out of the injection needle 11 and flows down along the aspiration needle 10. Under the guidance of the aspiration needle 10, the liquid flows down obliquely along the curved top of the aspiration needle 10, and will not directly impact the cell layer vertically, thereby preventing damage to the cell layer.

[0084] Further, such as Figure 5 As shown, the liquid suction and injection components 8 are arranged in groups of 1 to 5 and installed on the robot arm 6 in a line.

[0085] Specifically, the liquid suction and injection components 8 are used to add reagents to the sample and absorb and discharge waste liquid. One group of liquid suction and injection components 8 can process one sample, and setting 1-5 groups of liquid suction and injection components 8 can process 1-5 samples at the same time, thereby improving sample processing efficiency.

[0086] As a possible implementation, Figure 1-4 As shown, the aspiration and injection mechanism 7 generates continuous positive and negative pressures, so that the disposable pipette tip 78 repeatedly aspirates and discharges the sample in the sample bottle 18, thereby achieving the purpose of resuspending and mixing the sample.

[0087] It is understood that commercially available disposable pipette tips come in a variety of sizes. The aspiration and injection mechanism 7 of this embodiment can be set to an appropriate negative pressure value based on the size of the disposable pipette tip, so that the volume of sample aspirated by the disposable pipette tip matches its size. That is, the aspirated sample is retained within the disposable pipette tip and is not drawn into the lumen of the cannula 9. Each disposable pipette tip processes a single sample from a single sample bottle and is discarded after use. The sample only contacts the disposable pipette tip and not the aspiration and injection mechanism, thus preventing contamination.

[0088] Further, such as Figure 8-10 As shown, the sample bottle push rod 19 includes a horizontal push rod 38 and a vertical push rod 39 , and the horizontal push rod 38 is located above the vertical push rod 39 .

[0089] The horizontal push rod 38 is located at the upper part of the sample bottle support seat 16, and includes a first vertical arm 40, a first horizontal arm 41 and a second driving device 42. The first vertical arm 40 and the first horizontal arm 41 are connected in a T shape. The first horizontal arm 41 is perpendicular to the radius of the centrifugal turntable 12. The second driving device 42 is connected to the first vertical arm 40, which can drive the horizontal push rod 38 to move toward the center of the centrifugal turntable 12, so that the first horizontal arm 41 contacts the upper part of the sample bottle in the sample bottle support seat 16.

[0090] The vertical push rod 39 is located below the sample bottle support 16, and includes a second vertical arm 43, a second horizontal arm 44 and a third driving device 45. The second vertical arm 43 and the second horizontal arm 44 are connected in a T shape. The second horizontal arm 44 is perpendicular to the radius of the centrifugal turntable 12. The third driving device 45 is connected to the second vertical arm 43, which can drive the vertical push rod 39 to move in a vertical upward direction, so that the second horizontal arm 44 contacts the sample bottle support 16.

[0091] After the horizontal push rod is pushed, it contacts the upper part of the side wall of the sample bottle, and can push the sample bottle in the horizontal direction, so that the sample bottle drives the sample bottle supporting seat to swing and tilt around the axis; after the vertical push rod is pushed, it contacts the lower part of the side wall of the inclined sample bottle supporting seat, and can push the sample bottle supporting seat in the vertical direction, so that the sample bottle supporting seat drives the sample bottle to continue to swing and tilt around the axis.

[0092] Traditional liquid-based cell slide preparation machines use suction to remove waste liquid (the supernatant after centrifugation). This involves a robotic arm driving a gas-liquid pipeline into each sample bottle, creating negative pressure to draw waste liquid from the sample bottle and transfer it to a waste liquid bottle. This method requires the coordination of pumps, valves, piping, and a robotic arm, resulting in a complex mechanical structure, numerous components, and a high failure rate.

[0093] Therefore, the present application abandons this suction-type method of discharging waste liquid and adopts a simple-structured pouring device to push the sample bottle over so that the waste liquid automatically flows out under the action of gravity.

[0094] Specifically, when the waste liquid (supernatant) needs to be dumped after centrifugation is completed, the second drive device 42 drives the first vertical arm 40 of the horizontal push rod 38 to move, and drives the first cross arm 41 to move toward the center of the centrifuge turntable 12, contacting the upper portion of the sample bottle holder 16. The second drive device 42 then drives the first vertical arm 40 of the horizontal push rod 38 to move, and drives the first cross arm 41 to move toward the center of the centrifuge turntable 12, contacting the upper portion of the sample bottle holder 16, and continuing to move, pushing the sample bottle holder 16 to swing about the rotation axis 17, causing the sample bottle holder 16 and the sample bottle 18 placed therein to swing and tilt about the rotation axis 17. At this time, the lower side of the sample bottle holder 16 is lifted above the vertical push rod. The third drive device 45 then drives the second vertical arm 43 of the vertical push rod 39 to move upward, and drives the second cross arm 44 to move upward, contacting the lower side of the tilted sample bottle holder 16, and continuing to move, pushing the sample bottle holder 16 to continue to swing along the rotation axis 17, driving the sample bottle 18 to continue to swing about the rotation axis 17, until the sample bottle mouth is lower than the bottle bottom, and the waste liquid flows out of the sample bottle 18 under the action of gravity. The horizontal push rod 38 and the vertical push rod 39 cooperate with each other to discharge the waste liquid in the sample bottle.

[0095] After the waste liquid is discharged, the vertical push rod 39 retreats and resets, the horizontal push rod 38 retreats and resets, and the sample bottle 18 and the sample bottle supporting seat 16 automatically return to the vertical state under the action of gravity.

[0096] It is understandable that the horizontal push rod 38 and the vertical push rod 39 constituting the sample bottle push rod 19 are both one piece and can be disposed at any position on the periphery of the centrifugal turntable 12 .

[0097] Specifically, as the centrifugal turntable 12 rotates, the sample bottles are sequentially moved to the position of the sample bottle push rod 19 and pushed down by the sample bottle push rod 19 to dump out the waste liquid. The sample bottle push rod 19 is set at any position on the periphery of the centrifugal turntable 12 and can push down any sample bottle through the rotation of the centrifugal turntable 12 to complete the waste liquid dumping work.

[0098] As a possible embodiment, the height of the sample bottle 18 is greater than the inner height of the sample bottle holder 16. When the sample bottle 18 is placed in the sample bottle holder 16, the upper portion of the sample bottle is higher than the sample bottle holder 16. The horizontal push rod 38 of the sample bottle push rod 19 can push the upper portion of the sample bottle 18 in the sample bottle holder 16, causing the sample bottle 18 to drive the sample bottle holder 16 to swing along the axis 17.

[0099] The benefit of such a setting is that when dumping waste liquid, the horizontal push rod 38 can directly push the sample bottle without pushing the sample bottle holder 16. Therefore, the sample bottle holder 16 can be connected to the axis of the centrifugal turntable 12 at the top. In this way, the center of gravity of the sample bottle holder 16 is at a position far below the axis. After the external force is removed, it is conducive to the sample bottle holder 16 to quickly return to the vertical state under the action of gravity.

[0100] Further, such as Figure 8-10 As shown, a fixing block 46 is provided in the middle of the second cross arm 44 . The size of the fixing block 46 is adapted to the distance between the two sample bottle supporting seats 16 and is used to fix the sample bottle supporting seats 16 .

[0101] It is understandable that the sample bottle push rod 19 can push one sample bottle at a time to complete the waste liquid dumping, and can also push two sample bottles at a time to complete the waste liquid dumping.

[0102] Pushing one sample bottle at a time is inefficient. Pushing two sample bottles at a time can double the efficiency. Both methods have a problem: the centrifugal turntable 12 itself is rotatable, and when the sample bottle push rod 19 pushes the sample bottle, the uneven force will cause the centrifugal turntable to rotate, causing the sample bottle to slip off the sample bottle push rod 19, affecting the dumping of waste liquid.

[0103] In this embodiment, a fixing block 46 is provided in the middle of the second horizontal arm 44. The size of the fixing block 46 is adapted to the distance between the two sample bottle holders 16. When the second horizontal arm 44 of the vertical push rod 39 contacts the sample bottle 18, the fixing block 46 is inserted between the two sample bottles 18 to fix the sample bottles 18, thereby preventing the centrifugal turntable 12 from continuing to rotate under the action of inertia, stabilizing the sample bottles 18, and making the pouring of the centrifugal supernatant (i.e., waste liquid) more convenient and easy to operate, thereby facilitating the waste liquid treatment.

[0104] Further, such as Figure 8-10 As shown, the waste liquid collecting device 47 is further included. The waste liquid collecting device 47 includes a waste liquid receiving tray 48, a second waste liquid pipeline 49 and a waste liquid bottle 28.

[0105] The waste liquid receiving tray 48 is arranged below the centrifugal turntable 12 and is annular; the waste liquid receiving tray 48 includes a tray bottom 50, an inner tray wall 51 and an outer tray wall 52, the inner tray wall 51 is located in a circle around the inner periphery of the tray bottom 50, and the outer tray wall 52 is located in a circle around the outer periphery of the tray bottom 50, and the inner tray wall 51 and the outer tray wall 52 protrude from the tray bottom 50, and the tray bottom 50, the inner tray wall 51 and the outer tray wall 52 together form an annular recessed space 53, which can be used to receive waste liquid; a waste liquid port 54 is provided on the tray bottom 50, which passes through from top to bottom; the lower end of the waste liquid port 54 is connected to the waste liquid bottle 28 through a second waste liquid pipeline 49, and a third valve 55 is provided on the pipeline, and the switch of the third valve 55 is controlled by the controller 31.

[0106] The waste liquid collecting device 47 is used to collect the supernatant (waste liquid) generated after the cell enrichment module 3 is centrifuged.

[0107] Specifically, after the waste liquid is poured out by the waste liquid dumping device 14 , it flows into the waste liquid receiving tray 48 . The third valve 55 is opened, and the waste liquid flows into the waste liquid bottle 28 through the waste liquid port 54 and the second waste liquid pipeline 49 .

[0108] The third valve 55 is usually closed and opened only when the waste liquid is dumped. On the one hand, it can reduce the volatilization of waste liquid in the waste liquid bottle into the environment and pollute the environment. On the other hand, it can provide a closed environment for the pipeline system of the tablet making machine, facilitating the generation of negative pressure and positive pressure in the pipeline system.

[0109] As a possible embodiment, the waste liquid port 54 is located below the sample bottle push rod 19. Such a setting is conducive to the rapid flow of waste liquid into the waste liquid bottle, shortening the time the waste liquid stays in the waste liquid receiving tray 48, and reducing environmental pollution.

[0110] As a possible implementation, Figure 8-10 As shown, an anti-slip pad 56 is provided on the outer disk wall 52 at a position corresponding to the sample bottle push rod assembly, which is used to limit the position of the sample bottle 18 after the sample bottle push rod assembly pushes the sample bottle 18 to a horizontal position.

[0111] It is understood that when the sample bottle 18 rests naturally in the sample bottle holder 16 without a retaining device, when the sample bottle 18 is tilted to its lowest position, it contacts the upper surface of the outer wall 52 of the waste liquid receiving tray 48. At this point, the bottle mouth is lower than the bottle bottom, making it easy for the sample bottle 18 to slip out of the sample bottle holder 16, resulting in subsequent sample transfer failure due to the sample bottle slipping out. Providing a non-slip pad with a high friction coefficient at the location where the sample bottle 18 contacts the upper surface of the outer wall 52 of the waste liquid receiving tray 48 can prevent the sample bottle 18 from slipping out of the sample bottle holder 16.

[0112] As a possible implementation, Figure 8-10As shown, a flushing pipe is also included. The flushing pipe is arranged above the waste liquid port 54 and is used to clean the waste liquid port 54 to prevent chemical reagent crystallization from clogging the waste liquid port 54 and to prevent chemical reagent residue from corroding the waste liquid receiving tray and the waste liquid port.

[0113] As a possible implementation, Figure 1-4 As shown, the cell enrichment module 3 has a sealing cover that folds open and close.

[0114] When placing the sample bottle, the sealing cover is opened without affecting the operation of the experimenter.

[0115] During centrifugation, the sealing cover is closed to seal the cell enrichment module 3, which can prevent the sample or sample bottle from being thrown out of the cell enrichment module 3 during centrifugation, and can also prevent the aerosol generated by centrifugation from evaporating into the environment, thereby protecting the health of the experimenters.

[0116] The foldable sealing cover takes up little space, and can reduce the size of the fully automatic liquid-based cell preparation machine 1 as a whole.

[0117] Further, such as Figure 1-4 As shown, the detection unit 20 includes a positioning sensor 57 and a bottle detection sensor 58. The positioning sensor 57 includes a first component 59 and a second component 60. The first component 59 is fixedly mounted on the centrifugal turntable 12 and rotates with the centrifugal turntable. The first component 59 and the second component 60 are used in conjunction to position the sample bottle holder 16.

[0118] The bottle detection sensor 58 is disposed on one side of the centrifugal turntable 12 , slightly higher than the sample bottle support 16 . The bottle detection sensor 58 determines whether there is a sample bottle in the sample bottle support 16 .

[0119] The positioning sensor 57 and the bottle detection sensor 58 cooperate to confirm the sample bottle support 16 where the leveling block needs to be placed.

[0120] Specifically, the installation positions of the bottle detection sensor 58 and the positioning sensor 57 can be pre-set so that the bottle detection sensor 58 and the positioning sensor 57 differ in circumferential direction by at least one station. The position of each sample bottle support 16 is a station.

[0121] An example is given in which 24 sample bottle carriers 16 are provided on the centrifugal turntable 12 and the positioning sensor 57 and the bottle detection sensor 58 are spaced 11 stations apart.

[0122] Component 1 59 rotates with the centrifugal turntable 12 and passes through component 2 60. Component 2 60 receives the signal from component 1 59 and transmits a signal to controller 31, recording the sample bottle holder 16 corresponding to the radius of component 1 as sample bottle holder 1. At this point, the sample bottle holder 16 detected by bottle detection sensor 58 is sample bottle holder 13. Bottle detection sensor 58 then begins detecting whether a sample bottle 18 is in sample bottle holder 13 and transmits this detection information to controller 31. As centrifugal turntable 12 rotates, bottle detection sensor 58 sequentially detects whether a sample bottle 18 is in each sample bottle holder and transmits this detection information to controller 31. This process continues until the centrifugal turntable 12 completes one rotation and the status of all sample bottles 18 in each sample bottle holder 16 is fully detected. Controller 31 compares the received information with the information of the sample bottles 18 in the symmetrical sample bottle holders 16, determines whether balance is necessary, and records the information of the sample bottle holders 16 that require balance.

[0123] Further, such as Figure 11 As shown, the balancing block 21 is a columnar structure with the same weight as the sample bottle 18 , with a non-through insertion hole 79 in the middle, and the diameter of the insertion hole 79 is slightly larger than the sleeve 9 of the liquid aspiration and injection component 8 .

[0124] Specifically, controller 31 controls transfer module 2 to move above balancing block 21 and lowers suction and injection mechanism 7, allowing cannula 9 to penetrate into insertion hole 79 of balancing block 21. After the suction cup on cannula 9 contacts the top of balancing block 21, suction and injection mechanism 7 creates a vacuum, firmly sucking the balancing block 21. Driven by transfer module 2, the balancing block 21 is transferred to the sample bottle holder 16 to be balanced, completing the balancing. After centrifugation, transfer module 2 transfers balancing block 21 to the storage holder.

[0125] As a possible implementation, each balancing block 21 has the same weight as three sample bottles 18 .

[0126] The purpose of such arrangement is that when manually placing the sample bottles 18, they can be placed in sequence along the sample position without paying special attention to balancing. After the self-balancing assembly 15 detects the information of the sample bottles 18, it automatically places the balancing block 21 in the sample bottle support seat 16 that needs balancing for balancing.

[0127] Take the processing of 12 samples as an example: 12 sample bottles 18 are placed in the sample bottle holders No. 1-12 in sequence. After the self-balancing component 15 detects the information of the sample bottle 18, it sends the status information of the sample bottle 18 to the controller 31. The controller 31 controls the transfer module 2 to move above the balancing block 21, and moves the suction and injection mechanism 7 downward to make the sleeve 9 penetrate into the socket 79 of the balancing block 21. After the suction cup on the sleeve 9 contacts the top of the balancing block 21, the suction and injection mechanism 7 draws vacuum to firmly suck the balancing block 21, and driven by the transfer module 2, the four balancing blocks 21 are transferred to 14, Balancing is completed in sample bottle holders 16 for bottles 17, 20, and 23. Specifically, the three sample bottles 18 in bottle holders 1, 2, and 3 are balanced with the balancing block 21 in bottle holder 14. The three sample bottles 18 in bottle holders 4, 5, and 6 are balanced with the balancing block 21 in bottle holder 17. The three sample bottles 18 in bottle holders 7, 8, and 9 are balanced with the balancing block 21 in bottle holder 20. The three sample bottles 18 in bottle holders 10, 11, and 12 are balanced with the balancing block 21 in bottle holder 23. After centrifugation is complete, transfer module 2 transfers balancing block 21 to the storage holder.

[0128] Further, such as Figure 1-4 As shown, the pipette tip module 4 includes a pipette tip seat 61 and a photoelectric sensor 62. The pipette tip seat 61 is used to carry a disposable pipette tip 78; the pipette tip seat 63 is provided on the pipette tip seat 61, and the pipette tip seat is a circular hole. The disposable pipette tip 78 can be stably placed in the circular hole of the pipette tip seat 63.

[0129] It is understandable that the disposable pipette tip 78 can be installed on the sleeve 9 of the transfer module 2 in a plug-in manner.

[0130] Specifically, the aspiration and injection mechanism 7 is driven by the robotic arm 6 to move above the pipette tip seat 61, moves downward so that the sleeve 9 of the aspiration and injection component 8 is inserted into the disposable pipette tip 78, and continues to move downward until the disposable pipette tip 78 is stably inserted on the sleeve 9.

[0131] The robotic arm 6 drives the aspiration and injection mechanism 7 to move to the cell enrichment module 3, so that the sleeve 9 penetrates into the sample bottle 18, and uses the disposable pipette tip 78 to aspirate a quantitative sample. The robotic arm 6 drives the aspiration and injection mechanism 7 to move to the slab preparation module 5, and injects the sample in the disposable pipette tip 78 into the slab preparation module 5 for slab preparation.

[0132] It can be understood that the photoelectric sensor 62 is arranged on the movement path of the transport module 2 from the pipette tip seat 61 to the cell enrichment module 3, and is used to detect whether the disposable pipette tip 78 is installed in place.

[0133] Specifically, after the disposable pipette tip 78 is inserted and installed in the cannula 9, the transport module 2 drives the disposable pipette tip 78 past the photoelectric sensor 62, which detects whether the pipette tip 78 is properly installed. If the pipette tip 78 is properly installed, the transport module 2 drives the disposable pipette tip 78 to continue moving to the cell enrichment module 3. If the pipette tip 78 is not properly installed, the transport module 2 returns to the pipette tip module 4 and re-inserts the pipette tip 78 until it is successfully installed. The transport module 2 then drives the disposable pipette tip 78 to continue moving to the cell enrichment module 3.

[0134] It is understandable that sample transfer relies on disposable pipette tips. The successful installation of disposable pipette tips directly determines the success of subsequent sample transfer, which in turn affects the success of slide preparation and staining. This embodiment performs a test during the installation step of the disposable pipette tip to ensure successful installation of the disposable pipette tip, thereby ensuring the success of subsequent sample transfer, slide preparation, and staining, thereby improving success rates and production efficiency.

[0135] As a possible implementation, the pipette tip module 4 is arranged between the cell enrichment module 3 and the slicing module 5 . Such an arrangement can reduce the movement distance of the transport module 2 .

[0136] As a possible implementation, the spacing of the pipette tip positions 63 is consistent with the spacing of the sleeves 9 of the aspiration and injection assembly 8. This arrangement allows all sleeves 9 to be plugged into the disposable pipette tips 78 at the same time.

[0137] Further, such as Figure 1-4 As shown, the film-making module 5 includes a workbench 64 and a film-making clamp assembly 65 .

[0138] The workbench 64 is composed of a plurality of workstations 66 , each of which is shaped like a groove adapted to the film clamp assembly 65 .

[0139] The slide clamp assembly 65 includes a slide holder 67 , a sedimentation chamber 68 and a slide 69 . A sealing rubber ring 70 is provided between the sedimentation chamber 68 and the slide 69 . The sedimentation chamber 68 is connected to the slide holder 67 via a clip 71 .

[0140] The slide preparation clamp is assembled as follows: a slide 69 is placed on a slide holder 67, and one end of a sedimentation chamber 68 with a sealing rubber ring 70 is placed on the slide 69. Sedimentation chamber 68, slide 69, and slide holder 67 are secured together using clips 71 on the sedimentation chamber 68 and the slide holder 67. The sealing rubber ring 70 forms a sealed preparation space between sedimentation chamber 68 and slide 69 for holding samples for slide preparation.

[0141] The film-making clamp assembly 65 can be placed in a station 66 and can move freely. The spacing between the centers of adjacent film-making stations 66 is consistent with the spacing between the sleeves 9 of the liquid suction and injection assembly 8.

[0142] The sedimentation preparation process is as follows: the transport module 2 transfers the sample from the cell enrichment module 3 to the sedimentation chamber 68. The sample is left to stand in the preparation space composed of the sedimentation chamber 68, the glass slide 69 and the glass slide holder 67. The cells are deposited on the surface of the glass slide 69 under the action of gravity, forming a circular cell layer with the same diameter as the sedimentation chamber 68.

[0143] The film-making clamp assembly 65 is placed in the film-making station 66 . Due to the obstruction of the side wall of the film-making station 66 and the fact that it is not fixed by any fastening measures, the film-making clamp assembly 65 can move freely in the space above the film-making station 66 .

[0144] Driven by the robotic arm 6, the liquid suction and injection component 8 moves downward, so that the lower end of the sleeve 9 enters the sedimentation bin 68, and the lower end of the liquid suction needle 10 stops at the corresponding position of the sedimentation bin 68. It can be lowered to the middle position of the sedimentation bin 68 during injection, and can be lowered to a position close to the bottom of the sedimentation bin 68 during liquid suction. The robotic arm 6 drives the aspiration assembly 8 to move horizontally to the right. When the lower end of the aspiration needle 10 strikes the inner wall of the sedimentation chamber 68, the sedimentation chamber 68 is pushed to the right by the aspiration needle 10. However, the slide holder 67 is blocked by the right side wall of the slide preparation station 66. The robotic arm 6 drives the aspiration assembly 8 to continue to move horizontally to the right. Under the continued driving force of the aspiration needle 10, the slide holder 67 rotates and tilts about its right end. However, due to the obstruction of the front and rear walls of the slide preparation station 66, the slide holder 67 cannot rotate in other directions. When the external force is eliminated, the slide holder 67 returns to its original position under the action of gravity. The same process is repeated when the aspiration assembly 8 moves horizontally to the left, front, and back.

[0145] The dyeing process is as follows: under the control of the controller 31, the liquid suction and injection component 8 injects different reagents into the production space in turn through different injection needles 11, and extracts the waste liquid generated during the production process from the production space through the liquid suction needle 10, and then introduces it into the waste liquid bottle 28.

[0146] During this process, sedimentation chamber 68 is tilted during injection and aspiration, and horizontal at other times. It is understood that during injection, the reagent flows down along the tilted sidewalls of sedimentation chamber 68, covering the cell layer, thereby preventing the reagent from directly impacting the cell layer and causing damage to the cell layer. During aspiration, the tilted sedimentation chamber 68 can collect waste liquid, maximizing its aspiration efficiency.

[0147] Further, such as Figure 1-4 As shown, it also includes a solid waste collection module 72, which includes a tube removal component 73 and a waste collection box 74. The tube removal component 73 is arranged directly above the waste collection box 74, and the solid waste collection module 72 is used to collect discarded disposable pipette tips 78.

[0148] The tube removal assembly 73 includes a support plate 75 and a gun removal plate 76. The gun removal plate 76 is mounted on the upper portion of the support plate 75. The gun removal plate 76 is a horizontally arranged plate-like structure having an escape portion 77. The escape portion 77 is a U-shaped opening. The opening of the escape portion 77 is larger than the diameter of the sleeve 9 and smaller than the diameter of the top of the disposable pipette tip 78. The size of the escape portion 77 gradually decreases from the opening inward to facilitate the sleeve 9 to enter the escape step. After the sleeve 9 enters the escape portion 77, the disposable pipette tip 78 can be stuck under the escape step 77.

[0149] The number of the tube assembly 73 is no less than that of the suction and injection assembly 8, and each suction and injection assembly 8 has a corresponding tube assembly 73 to remove the disposable pipette tip 78. The number of the tube assembly 73 is preferably the same as that of the suction and injection assembly 8.

[0150] The spacing between adjacent tube removal components 73 is consistent with the spacing between the sleeves 9 of the liquid suction and injection component 8 to ensure that different sleeves 9 enter the corresponding tube removal components 73 at the same time and the disposable pipette tips 78 on all sleeves 9 are removed at the same time.

[0151] Specifically, the robotic arm 6 drives the liquid suction and injection mechanism 7 to the front end of the avoidance portion 77 of the tube removal assembly 73, and makes the sleeve 9 of the liquid suction and injection assembly 8 enter the interior of the gun removal plate 76 from the avoidance portion 77. The robotic arm 6 drives the liquid suction and injection mechanism 7 to move upward, so that the upper end of the disposable pipette tip 78 contacts the gun removal plate 76, is blocked by the gun removal plate 76, and cannot continue to move upward with the liquid suction and injection mechanism 7, thereby falling off the sleeve 9 and falling into the waste collection box 74 directly below the tube removal assembly 73.

[0152] On the other hand, a method for preparing a cell slide is also provided, which is prepared using the above-mentioned fully automatic liquid-based cell slide making machine, comprising the following steps:

[0153] S1, sample enrichment: First, the cells and other diagnostic components are deposited at the bottom tip of the sample bottle 18 through the centrifugal action of the cell enrichment module 3; then the supernatant after centrifugation is poured out by the waste liquid dumping device 14; finally, the reagent is injected into the cell sediment through the suction and injection mechanism 7 of the transport module 2, and the sample is mixed by suction.

[0154] S2, sample transfer: the suction and injection mechanism 7 of the transport module 2 generates negative pressure to suck a certain amount of mixed sample into the disposable pipette tip 78, and transports the sample to the sedimentation chamber 68 of the preparation module 5.

[0155] S3, sedimentation preparation: the sample in the sedimentation chamber 68 is allowed to stand for 5-20 minutes, and the cells and other diagnostic components in the sample naturally settle onto the glass slide 69 under the action of gravity.

[0156] S4, staining: the liquid injection mechanism 7 of the transport module 2 injects and extracts different reagents such as dye solution, alcohol, water, buffer solution and transparent solution into the sedimentation chamber 68 of the preparation module 5 multiple times to complete the staining and transparency of the cells.

[0157] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus comprising the element.

[0158] The embodiments of the present invention are described above in conjunction with the accompanying drawings, but the present invention is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present invention, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present invention and the claims, all of which are within the protection of the present invention.

Claims

1. A waste liquid dumping device, characterized in that: It includes a sample bottle push rod and a sample bottle supporting seat; The sample bottle supporting seat is used to support the sample bottle. Axes are provided on both sides of the upper part of the sample bottle supporting seat and can swing around the axes. The sample bottle push rod is arranged on one side of the sample bottle supporting seat and can push the sample bottle supporting seat to swing; The sample bottle push rod includes a horizontal push rod and a vertical push rod, and the horizontal push rod is located above the vertical push rod; after the horizontal push rod is pushed, it contacts the upper part of the side wall of the sample bottle, and can push the sample bottle in the horizontal direction, so that the sample bottle drives the sample bottle supporting seat to swing and tilt around the axis; after the vertical push rod is pushed, it contacts the lower part of the side wall of the inclined sample bottle supporting seat, and can push the sample bottle supporting seat in the vertical direction, so that the sample bottle supporting seat drives the sample bottle to continue to swing and tilt around the axis.

2. The waste liquid dumping device according to claim 1, characterized in that: The horizontal push rod is located on the upper part of the sample bottle supporting seat, and includes a first vertical arm, a first horizontal arm and a second driving device. The first vertical arm and the first horizontal arm are connected in a T-shape. The first horizontal arm is perpendicular to the radius of the centrifugal turntable. The second driving device is connected to the first vertical arm. The vertical push rod is located below the sample bottle supporting seat, and includes a second vertical arm, a second horizontal arm and a third driving device. The second vertical arm and the second horizontal arm are connected in a T shape. The second horizontal arm is perpendicular to the radius of the centrifugal turntable. The third driving device is connected to the second vertical arm.

3. The waste liquid dumping device according to claim 2, characterized in that: A fixing block is provided in the middle of the second cross arm, and the size of the fixing block is adapted to the distance between the two sample bottle supporting seats.

4. The waste liquid dumping device according to claim 1, characterized in that: It also includes a waste liquid collection device, which includes a waste liquid receiving tray, a second waste liquid pipeline and a waste liquid bottle; The waste liquid receiving tray is arranged below the centrifugal turntable and is in the shape of a ring. The waste liquid receiving tray includes a tray bottom, an inner tray wall and an outer tray wall. The inner tray wall is located in a circle around the inner periphery of the tray bottom, and the outer tray wall is located in a circle around the outer periphery of the tray bottom. The inner tray wall and the outer tray wall protrude from the tray bottom. The tray bottom is provided with a waste liquid port that passes through from top to bottom. The lower end of the waste liquid port is connected to the waste liquid bottle through a second waste liquid pipeline. A third valve is provided on the pipeline, and the switch of the third valve is controlled by a controller.

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