Chemostat system for cultivating algae

Abstract

The utility model relates to the field of algae cultivation equipment, in particular to a chemostat system for algae cultivation, which comprises a gas conveying device, a cultivation device, a liquid conveying device and a collecting device, the cultivation device comprises a cultivation bottle; the gas transmission device comprises a gas supply component; the air supply part comprises an air supply part and a carbon dioxide supply part which are connected with the cultivation bottle; the infusion device comprises a nutrient solution storage barrel; a nutrient solution is stored in the nutrient solution storage barrel; the nutrient solution storage barrel is connected with the cultivation bottle; the collecting device comprises a collecting bottle; according to the chemostat system disclosed by the utility model, closed culture can be realized, and the influence of external bacteria on algae culture is reduced; meanwhile, through cooperative use of air and carbon dioxide, the algae cultivation efficiency can be improved, and the experiment process is accelerated.

Description

Technical Field

[0001] The utility model relates to the field of algae cultivation equipment, in particular to a chemostat system for algae cultivation. Background Art

[0002] Microalgae are the main producers in natural water bodies. They are a type of tiny, photosynthetic, autotrophic, single-celled aquatic lower plants that play an important role in global energy conversion and carbon cycle. They can fix large amounts of carbon dioxide in the photosynthetic autotrophic process. They are also rich in high-value-added bioactive substances such as proteins, pigments, and various unsaturated fatty acids. They have important development prospects in the fields of aquatic bait, nutritional health products, biopharmaceuticals, and sewage treatment.

[0003] At present, there are two main types of artificial cultivation of microalgae: open and closed.

[0004] Open culture causes microalgae to come into direct contact with the external environment and obtain carbon dioxide from the outside air. Therefore, invasive species such as bacteria are inevitable, which leads to major problems with the purity of the microalgae.

[0005] For the closed culture mode, there are mainly specific photobioreactor styles such as pipeline type and flat plate type; for example, the content disclosed in 202310956323.7 - A constant temperature continuous culture device for microalgae.

[0006] Among them, the pipeline photobioreactor is widely used in the industrial cultivation of microalgae. During the cultivation process, a process pump is used as a circulation power to drive the circulation of microalgae. However, there are problems such as the algae easily adhering to the wall during growth, difficult to clean, high investment cost, and unsuitability for laboratory application.

[0007] Therefore, in order to improve or solve at least one of the above problems, it is necessary to optimize the design of the existing culture device. Utility Model Content

[0008] The utility model aims to provide a closed chemostat system which can reduce the influence of external bacteria on algae cultivation.

[0009] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0010] A chemostat system for algae cultivation includes a gas delivery device, a cultivation device, a liquid delivery device, and a collection device;

[0011] The cultivation device includes a cultivation bottle;

[0012] The gas delivery device includes a gas supply component;

[0013] The gas supply component includes an air supply component and a carbon dioxide supply component connected to the culture bottle;

[0014] The air supply component and the carbon dioxide supply component are arranged in parallel;

[0015] The infusion device includes a nutrient solution storage barrel; the nutrient solution storage barrel stores nutrient solution; the nutrient solution storage barrel is connected to the culture bottle;

[0016] The collecting device includes a collecting bottle;

[0017] The gas delivery device, the liquid delivery device and the collection device are all connected to the culture bottle via a pipeline device.

[0018] The pipeline device includes an air delivery pipe and a carbon dioxide delivery pipe;

[0019] The air delivery pipe and the carbon dioxide delivery pipe are both provided with a pressure regulating valve and a thermal mass flow meter.

[0020] The amount of carbon dioxide gas supplied into the culture bottle by the carbon dioxide supply component is greater than the amount of air gas supplied into the culture bottle by the air supply component.

[0021] The chemostat system further comprises a sampling device; the sampling device comprises a sampling bottle; the pipeline device comprises a sampling pipeline, the sampling bottle is connected to the culture bottle through the sampling pipeline; the sampling pipeline is connected to a syringe.

[0022] The culture bottle is provided with a reserved pipeline device, and the reserved pipeline device includes a shower-type air outlet pipe arranged on the culture bottle.

[0023] The pipeline device also includes an air supply pipeline and a liquid supply pipeline; the air supply component is connected to the culture bottle through the air supply pipeline, and the nutrient solution storage barrel is connected to the culture bottle through the liquid supply pipeline; the air supply pipeline is also connected to the liquid supply pipeline through a bridging pipeline; the bridging pipeline is provided with a first water stop valve.

[0024] The gas delivery device further comprises an air humidifier, and the gas supply component is connected to the culture bottle via the air humidifier.

[0025] The gas delivery device further comprises a pressure-dividing valve; the air humidifier is connected to the culture bottle via the pressure-dividing valve.

[0026] The culture bottle is connected to a magnetic stirrer.

[0027] The advantages of the present invention are:

[0028] The utility model discloses a chemostat system for cultivating algae.

[0029] The chemostat system disclosed in the utility model can realize closed culture and reduce the influence of external bacteria on algae cultivation; at the same time, the utility model can improve the efficiency of algae cultivation and accelerate the experimental process through the coordinated use of air and carbon dioxide. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The following is a brief description of the contents and marks in the drawings of the utility model specification:

[0031] Figure 1 It is a structural schematic diagram of the first embodiment of the present utility model.

[0032] Figure 2 This is a schematic structural diagram of the second embodiment of the present invention.

[0033] Figure 3 This is a schematic structural diagram of the third embodiment of the present invention.

[0034] Figure 4 This is a structural diagram of the arrangement of the first water stop valve and the second water stop valve in the present utility model.

[0035] Figure 5 This is a schematic diagram of the structure of the cultivation bottle and peripheral devices when connected in the present invention.

[0036] Figure 6 It is a structural schematic diagram of the liquid supply pipeline in the utility model.

[0037] The marks in the above figure are:

[0038] 1-1, gas transmission device; 1-2, liquid infusion device, 1-3, cultivation device, 1-4, collection device, 1-5, reserved pipeline device, 1-6, sampling device;

[0039] 151. Single pipe fittings, 152. Spherical pipes;

[0040] 1-Air delivery pipe; 2-Carbon dioxide delivery pipe; 3-Pressure regulating valve; 4-Mixing pipe; 5-Thermal mass flowmeter; 6-Air supply pipe; 7-Humidifier; 8-Pressure dividing valve; 9-Syringe filter; 10-Flow rate controller; 11-Nutrient solution storage tank; 12-Syringe; 13-Sampling bottle; 14-Bridge pipe; 15-Liquid supply pipe; 16-Cultivation bottle; 17-Magnetic stirrer; 18-Collecting bottle; 19-Shower-type air outlet pipe; 20-First water stop clamp; 21-Second water stop clamp; 22-Sampling pipe. DETAILED DESCRIPTION

[0041] The following describes the preferred embodiment with reference to the accompanying drawings to further illustrate the specific implementation of the present invention.

[0042] A chemostat system for algae cultivation comprises a gas delivery device 1-1, a cultivation device 1-3, a liquid delivery device 1-2 and a collection device 1-4; the utility model discloses a chemostat system for algae cultivation; through the coordinated use of the gas delivery device 1-1, the cultivation device 1-3, the liquid delivery device 1-2 and the collection device 1-4, the chemostat system disclosed in the utility model can achieve closed cultivation, thereby reducing the impact of external bacteria on algae cultivation.

[0043] Specifically, the chemostat system disclosed in the present invention is mainly suitable for cultivating algae in a laboratory to facilitate subsequent experimental operations; of course, if the material and size of the device are changed as needed, it can also be applied to other production needs.

[0044] Specifically, the cultivation device 1-3 disclosed in the present invention includes a cultivation bottle 16; in the present invention, the cultivation bottle 16 is an algae production site, and in the present invention, the cultivation bottle 16 can be a conical bottle with ears.

[0045] In addition, the gas delivery device 1-1 in the present invention includes an air supply component; in the present invention, the air supply component is mainly used to provide the gas required for algae growth for the closed chemical chamber system; at the same time, in the present invention, the air supply component is connected to the culture bottle 16 through an air supply component and a carbon dioxide supply component; in the present invention, the air supply component and the carbon dioxide supply component can be directly connected to the culture bottle 16, and of course it is best to connect them to the culture bottle 16 through the air humidifier 7 and the pressure dividing valve 8 below; this can reduce the impact of the supplied gas on the algae in the culture bottle 16.

[0046] In addition, in the present invention, the air supply component and the carbon dioxide supply component are arranged in parallel; such an arrangement can realize the separate supply of air and carbon dioxide, which is convenient for controlling their respective supply amounts during subsequent use. Moreover, the present invention can improve the efficiency of algae cultivation and speed up the experimental process by using air and carbon dioxide in combination.

[0047] In addition, it should be noted that although the air supply component and the carbon dioxide supply component described in the present invention are arranged in parallel, the gas is supplied separately in the early stage, and when the subsequent gas enters the cultivation bottle 16, the air and carbon dioxide are mixed with each other before entering; it is equivalent to supplying air with a high concentration of carbon dioxide into the cultivation bottle 16.

[0048] In the present invention, the infusion device 1-2 includes a nutrient solution storage barrel 11; the nutrient solution storage barrel 11 stores nutrient solution; the nutrient solution storage barrel 11 is connected to the culture bottle 16; in the present invention, the nutrient solution can provide basic nutrition for the production of algae and ensure the growth of algae.

[0049] In the present invention, the collecting device 1-4 includes a collecting bottle 18; in the present invention, the collecting bottle 18 is mainly used to collect the grown algae, and can also serve as an exhaust to avoid excessive gas pressure in the cultivation bottle 16.

[0050] In the present invention, the gas delivery device 1-1, the liquid delivery device 1-2, and the collection device 1-4 are connected to the culture bottle 16 through a pipeline device; such an arrangement can facilitate the installation and layout of the entire chemostat system; the pipeline device can include the following pipeline structures such as the air delivery pipe 1, the carbon dioxide delivery pipe 2, the gas supply pipe 6, the liquid supply pipe 15, the sampling pipe 22, etc. The pipeline device plays a connecting and communicating role, facilitating the connection and communication between the corresponding components.

[0051] Furthermore, the pipeline device in the present invention includes an air delivery pipe 1 and a carbon dioxide delivery pipe 2; the air delivery pipe 1 and the carbon dioxide delivery pipe 2 of the present invention are mainly used to connect the air supply part and the carbon dioxide supply part; the air supply part can be an air tank or a compressor and other structures, and the carbon dioxide supply part is a carbon dioxide supply tank.

[0052] At the same time, in the utility model, a pressure regulating valve 3 and a thermal mass flow meter 5 are provided on the air delivery pipe 1 and the carbon dioxide delivery pipe 2; the function of the pressure regulating valve 3 of the utility model is to control the gas pressure in the air delivery pipe 1 or the carbon dioxide delivery pipe 2, so as to facilitate the subsequent supply of gas of different pressures as needed; and the thermal mass flow meter 5 is used to identify the flow rate of the gas delivered by the air delivery pipe 1 or the carbon dioxide delivery pipe 2, so as to facilitate accurate identification and monitoring.

[0053] Furthermore, in the present invention, the amount of carbon dioxide gas supplied by the carbon dioxide supply component to the cultivation bottle 16 is greater than the amount of air gas supplied by the air supply component to the cultivation bottle 16; such a setting can ensure the supply of sufficient amount of carbon dioxide and avoid the impact of too low carbon dioxide on the production speed of algae; at the same time, during actual gas supply, the supply ratio of pure carbon dioxide to air is between 1.2-2; the preferred ratio is 1.7 to 1.

[0054] In the present invention, the chemostat system further comprises sampling devices 1-6. By providing the sampling devices 1-6, the present invention can extract corresponding samples at different stages during the cultivation process, thereby facilitating actual detection operations.

[0055] Specifically, in the present invention, the sampling device 1-6 includes a sampling bottle 13; the pipeline device includes a sampling pipeline 22, and the sampling bottle 13 is connected to the culture bottle 16 through the sampling pipeline 22; the sampling pipeline 22 is connected to the syringe 12; and a second water stop valve 21 is provided on the sampling pipeline 22; the present invention can control the on and off of the sampling pipeline 22 through the second water stop valve 21. During subsequent use, the syringe 12 is also connected to the culture bottle 16 through the sampling pipeline 22. The syringe 12 extracts a corresponding amount of liquid through the sampling pipeline 22. Subsequently, the second water stop valve 21 is opened, and the liquid in the syringe 12 enters the sampling bottle 13 along the sampling pipeline 22 under the influence of gravity, thereby realizing the corresponding sampling operation.

[0056] In addition, in the present invention, the syringe 12 can also inject growth elements required by algae into the culture bottle 16, which is helpful to accelerate the growth rate of algae; when injecting growth elements, the second water stop valve 21 on the sampling pipe 22 is required to be in a closed state.

[0057] Furthermore, in the present invention, a reserved pipeline device 1-5 is provided on the culture bottle 16, and the reserved pipeline device 1-5 includes a shower-type air outlet pipe 19 arranged on the culture bottle 16; in the present invention, the reserved pipeline device 1-5 has one function of realizing the exhaust operation of the culture bottle 16 and reducing the gas pressure in the culture bottle 16, and another function is to act as an external component to facilitate the connection of the culture bottle 16 to other components.

[0058] In the present invention, the pipeline device also includes an air supply pipeline 6 and a liquid supply pipeline 15; the air supply component is connected to the culture bottle 16 through the air supply pipeline 6, and the nutrient solution storage barrel 11 is connected to the culture bottle 16 through the liquid supply pipeline 15; the setting of the liquid supply pipeline 15 and the air supply pipeline 6 can respectively realize the connection and communication between the infusion device 1-2 and the air supply device 1-1 and the culture bottle 16.

[0059] In the present invention, the gas supply pipe 6 is also connected to the liquid supply pipe 15 through a bridging pipe 14; a first water stop valve 20 is provided on the bridging pipe 14; the setting of the bridging pipe 14 of the present invention plays a reflux role. When the gas pressure supplied by the gas transmission device 1-1 is too low, a reflux problem may occur. At this time, the first water stop valve 20 is opened to allow the algae to flow into the cultivation bottle 16 again along the liquid supply pipe 15.

[0060] In the present invention, the liquid supply pipeline 15 includes a plurality of single pipe fittings 151, and adjacent single pipe fittings 151 can be connected by a spherical tube 152 with a hollow interior. Such a setting makes the liquid supply pipeline 15 a pipe structure with a variable inner diameter, which can reduce the flow rate of the liquid during liquid supply. In other words, the design of the spherical tube 152 can reduce the liquid supply speed and avoid the newly supplied nutrient solution from causing excessive impact on the algae in the culture bottle 16.

[0061] Furthermore, in the present invention, the gas supply device 1-1 also includes an air humidifier 7, and the gas supply component is connected to the culture bottle 16 through the air humidifier 7; in the present invention, the basic function of the air humidifier 7 is to humidify the gas to be entered into the culture bottle 16, so as to avoid the loss of liquid in the culture bottle 16 when dry air enters the subsequent culture bottle 16. If the loss continues, it will affect the growth of algae. Therefore, the gas to be entered into the culture bottle 16 is humidified, and after being humidified, it is passed into the culture bottle 16, which can reduce or avoid the loss of water in the culture bottle 16 due to the supply of gas.

[0062] In addition, another function of the air humidifier 7 is to act as a gas mixture, so that air and pure carbon dioxide are mixed in the air humidifier 7 and then enter the subsequent pipeline.

[0063] In addition, the present invention can also achieve a sterilization operation on the supplied gas by limiting the medium in the air humidifier 7. Of course, through the obstruction of the medium, it also has a good impurity removal effect, preventing impurities in the air from entering subsequent devices.

[0064] Furthermore, in the present invention, the gas delivery device 1-1 also includes a pressure-dividing valve 8; the air humidifier 7 is connected to the cultivation bottle 16 through the pressure-dividing valve 8; the setting of the pressure-dividing valve 8 can control the pressure of the gas subsequently entering the cultivation bottle 16. In the present invention, the pressure of the gas entering the cultivation bottle 16 is generally required to be around 0.2kPa; at the same time, the pressure-dividing valve 8 also plays a good diversion external connection role, which is convenient for subsequent use and multiple cultivation bottles 16 can be connected at the same time to improve the cultivation efficiency.

[0065] Furthermore, in the present invention, the culture bottle 16 is connected to a magnetic stirrer 17 ; the culture bottle 16 is placed above the magnetic stirrer 17 , and the culture bottle 16 is shaken to prevent algae from growing on the wall.

[0066] Furthermore, in the present invention, the cultivation bottle 16 is placed next to simulated sunlight, so that the algae can use light energy to convert carbon dioxide and water into organic matter for their own growth. Different algae require different lighting conditions, and the light intensity should be adjusted according to actual conditions.

[0067] A method for cultivating algae based on the chemostat system, the method comprising the following steps:

[0068] Step 1: Sterilize the chemostat system first;

[0069] Step 2: After completing step 1, immediately proceed with assembling the chemostat system;

[0070] Step 3: Introduce the inoculated algae solution and nutrient solution into the reactor; the gas supply device 1-1 introduces air and carbon dioxide into the culture bottle 16;

[0071] Supplement nutrient solution and corresponding gas according to experimental needs;

[0072] Until the experimental requirements are met;

[0073] Step 4: After step 3 is completed, the algae cultivation is completed. If new algae cultivation is required, repeat steps 1-3 above.

[0074] The utility model can realize the cultivation of algae through the above-mentioned cultivation method.

[0075] At the same time, the present invention performs a device sterilization operation before assembling the chemostat system, thereby avoiding the risk of external bacteria entering the chemostat system and reducing the impact of external bacteria on algae cultivation.

[0076] At the same time, in the utility model, when the gas transmission device 1-1 actually transmits gas, pure carbon dioxide gas and air are first supplied separately, and then the two are mixed with each other, and finally enter the cultivation bottle 16 through partial pressure. This operation method can not only ensure the air intake volume of the cultivation bottle 16, but also ensure the carbon dioxide concentration in the intake air.

[0077] specific:

[0078] The utility model discloses a chemostat system for cultivating algae. The chemostat system comprises a gas delivery device 1-1, a cultivation device 1-3, a liquid delivery device 1-2, a sampling device 1-6 and a collection device 1-4.

[0079] Specifically, it includes an air delivery pipe 1, a carbon dioxide delivery pipe 2, a pressure regulating valve 3, a thermal mass flow meter 5, an air humidifier 7, a pressure dividing valve 8, a needle filter 9, a nutrient solution storage barrel 11, a flow rate control valve, a cultivation bottle 16, a collection bottle 18, sampling devices 1-6, a shower-type air outlet pipe 19, etc.

[0080] In the present invention, the gas delivery device 1 - 1 , the liquid delivery device 1 - 2 , the sampling device 1 - 6 and the collection device 1 - 4 are all connected to a cultivation bottle 16 , and the cultivation bottle 16 is connected to an algae collection bottle 18 .

[0081] The air delivery pipe 1 and the carbon dioxide delivery pipe 2 are provided with a pressure regulating valve 3, the purpose of which is to regulate and stabilize the pressure in the pipeline system through the pressure regulating valve 3. It can maintain the stability of the system in the event of sudden pressure fluctuations or valve closure, thereby improving the reliability and safety of each device in the system.

[0082] After the gas passes through the pressure regulating valve 3, it passes through the thermal mass flow meter 5. The thermal mass flow meter 5 consists of a power supply, a reading application and a set value controller, and a metal-sealed thermal mass flow controller. By regulating the gas flow, the optimal mixing ratio of air and carbon dioxide gas can be achieved.

[0083] In the present invention, the air delivery pipe 1 and the carbon dioxide delivery pipe 2 can be directly connected to the air humidifier 7, or they can be connected to the humidifier 7 through a mixing pipe 4. Here, the mixing pipe 4 can also realize the gas mixing operation. In the present invention, the air delivery pipe 1 and the carbon dioxide delivery pipe 2 are connected to the mixing pipe 4 through a three-way interface / four-way interface, and then connected to the humidifier 7.

[0084] Specifically, in the present invention, air and carbon dioxide flow through different thermal mass flow meters 5 and then through a three-way interface / four-way interface to mix the air and carbon dioxide; and the pre-mixed gas flows through a humidifier 7 to make the air and carbon dioxide mixed gas moist, which is beneficial to the subsequent photosynthesis of algae.

[0085] After the mixed gas flows through the humidifier 7, it flows through the pressure-dividing valve 8 for pressure division and enters the air supply pipe 6. The air supply pipe 6 is provided with a needle filter 9, which plays a filtering role, and the air supply pipe 6 is connected to the culture bottle 16.

[0086] In addition, in the present invention, the mixed gas flows through the pressure-dividing valve 8 and the needle filter 9 and then enters the cultivation bottle 16, so that the pressure-dividing valve 8 leading to each algae culture vessel is stabilized at about 0.2 kPa.

[0087] The infusion device 1-2 of the present invention mainly includes a nutrient solution storage barrel 11, which is connected to a culture bottle 16 through a liquid supply pipe 15. At the same time, a flow rate controller 10 is provided on the liquid supply pipe 15. The flow rate controller 10 controls the supply amount of the nutrient solution and is used to control the flow rate of the nutrient solution so that the growth rate of the algae is always lower than its maximum growth rate for growth and reproduction.

[0088] At the same time, in order to avoid backflow when the air supply component is under low pressure, the present invention requires that a bridge pipe 14 be provided between the air supply pipe 6 and the liquid supply pipe 15 , and a first water stop valve 20 be provided on the bridge pipe 14 .

[0089] In the present invention, the sampling device 1-6 consists of a syringe 12, a sampling bottle 13 and a needle filter 9; the syringe 12 and the sampling bottle 13 are connected to the culture bottle 16 through a three-way interface, and the needle filter 9 is connected to the sampling bottle 13 to isolate bacteria in the outside air.

[0090] The algae collecting bottle 18 of the present invention is connected to the cultivation bottle 16 for collecting algae liquid.

[0091] The shower-type air outlet pipe 19 is connected to the culture bottle 16 to discharge excess air, prevent liquid from overflowing, and maintain the stability of the culture bottle 16.

[0092] The culture bottle 16 is placed on a magnetic stirrer 17, which is used to shake the culture bottle 16 to prevent algae from growing on the wall.

[0093] The chemostat system disclosed by the utility model has a simple structure, low investment cost, high culture efficiency, short cycle, eliminates the influence of natural air on algae growth, and is suitable for algae cultivation and production in laboratories.

[0094] Furthermore, in the present invention, the air delivery pipe 1 and the carbon dioxide delivery pipe 2 pass through the pressure regulating valve 3 and the thermal mass flow meter 5 to mix the air with the carbon dioxide.

[0095] Furthermore, in the present invention, the air pressure in the air delivery pipe 1 is about 1.0 kPa, and the carbon dioxide pressure in the carbon dioxide delivery pipe 2 is about 1.7 kPa.

[0096] Furthermore, in the present invention, there are two thermal mass flow meters 5, which are composed of a power supply, a reading application and a set value controller and a metal sealed thermal mass flow controller to monitor and regulate the air and carbon dioxide flows respectively.

[0097] Furthermore, in the present invention, after the two gases are mixed, they flow through the humidifier 7 through the three-way / four-way interface, so that the mixed gas of air and carbon dioxide becomes humidified, which is beneficial to the subsequent photosynthesis of algae.

[0098] Furthermore, in the present invention, after the mixed gas flows through the humidifier 7, it is pressure-divided by the pressure-dividing valve 8 and then filtered by the needle filter 9 and connected to the culture bottle 16 through the liquid supply pipe 15 via a three-way interface.

[0099] Furthermore, in the present invention, the mixed gas flows through the pressure-dividing valve 8 and the needle filter 9 and then enters the cultivation bottle 16, so that the pressure-dividing valve 8 leading to each algae culture vessel is stabilized at about 0.2 kPa.

[0100] Furthermore, in the present invention, the nutrient solution storage barrel 11 is connected to the chemostat through the flow rate controller 10. After the nutrient solution in the nutrient solution storage barrel 11 is sterilized, it is placed at a high place and flows to the flow rate controller 10 by gravity.

[0101] The flow rate controller 10 is used to control the flow rate of the nutrient solution so that the algae can always grow and reproduce at a rate lower than its maximum growth rate.

[0102] The chemostat system disclosed by the utility model is suitable for algae cultivation in a laboratory. The system has a simple structure, is easy to operate, saves manpower, and allows the algae to grow and reproduce at a growth rate always lower than its maximum growth rate, thereby accelerating the laboratory algae cultivation speed.

[0103] By connecting carbon dioxide and air, the influence of external air on algae growth is isolated, thereby avoiding the invasion of bacteria and other organisms during algae growth.

[0104] The chemostat system of the present invention can increase or decrease the number of culture bottles 16 as needed, and is flexible and changeable.

[0105] Furthermore, through the sampling device 1-6, not only can algae liquid be extracted from the culture bottle 16 and put into the sampling bottle 13 for use at any time, but growth factors can also be injected into the culture bottle 16. Specific embodiment:

[0107] With reference to the accompanying drawings, the present invention discloses a chemostat system for algae cultivation, which includes a gas delivery device 1-1, a cultivation device 1-3, a liquid delivery device 1-2, a sampling device 1-6, and a collection device 1-4.

[0108] Specifically, it includes an air delivery pipe 1, a carbon dioxide delivery pipe 2, a pressure regulating valve 3, a thermal mass flow meter 5, an air humidifier 7, a pressure dividing valve 8, a needle filter 9, a nutrient solution storage barrel 11, a flow rate control valve, a cultivation bottle 16, a collection bottle 18, sampling devices 1-6, a shower-type air outlet pipe 19, etc.

[0109] In the present invention, the gas delivery device 1 - 1 , the liquid delivery device 1 - 2 , the sampling device 1 - 6 and the collection device 1 - 4 are all connected to a cultivation bottle 16 , and the cultivation bottle 16 is connected to an algae collection bottle 18 .

[0110] Before use;

[0111] The entire culture bottle 16 and all pipeline devices need to be sterilized in a high-pressure sterilizer, including the nutrient solution storage barrel 11 filled with nutrient solution and the sampling bottle 13. Different parts require different sterilization conditions to provide a clean and sterile culture environment for algae strains or algae cultivation.

[0112] After sterilization, all pipelines are connected while the solution is still hot. The inoculum algae solution and nutrient solution are introduced into the reactor. The gas supply device 1-1 of the chemostat system introduces air and carbon dioxide into the culture bottle 16 respectively. The pressure in the gas supply part is adjusted and stabilized by the pressure regulating valve 3 to ensure that the system can maintain stability in the event of sudden pressure fluctuations or valve closure. The flow rate of each gas is then recorded and controlled by the thermal mass flow meter 5.

[0113] Then, the mixture is connected to the mixing pipe 4 through the three-way interface / four-way interface, the gases are mixed, passed into the air humidifier 7 for re-mixing of the gases, and the gases are humidified, passed into the pressure divider valve 8 and the needle filter 9, and then connected to the culture bottle 16.

[0114] The infusion device 1-2 of the chemostat system is filled with nutrient solution; the nutrient solution storage barrel 11 is connected to the flow rate controller 10 and then connected to the culture bottle 16. The nutrient solution plastic barrel is placed at a high place and relies on gravity to flow to the flow rate controller 10 and then flows into the liquid supply pipe 15 to connect to the culture bottle 16. By controlling the flow rate of the nutrient solution, the growth rate of the algae is always kept below its maximum growth rate for growth and reproduction.

[0115] The sampling device 1-6 of the chemostat system includes a syringe 12, a sampling bottle 13 and a needle filter 9. The syringe 12 can be used to extract algae liquid for preliminary experiments, and the growth elements required for algae can be injected into the culture bottle 16.

[0116] The shower-type air outlet pipe 19 is connected to the culture bottle 16 and is used to remove excess gas and maintain a stable air pressure in the bottle.

[0117] The chemostat system, through the combined action of the gas delivery device 1-1, the liquid delivery device 1-2, the shower-type air outlet pipe 19, the magnetic stirrer 17 at the bottom of the culture bottle 16 and the simulated sunlight, allows the algae to grow and reproduce at a rate lower than their maximum growth rate.

[0118] The algae liquid produced by the cultivation bottle 16 is collected by the algae collection bottle 18 .

[0119] Specifically, the air pressure in the air delivery pipe 1 is about 1.0 kPa, and the carbon dioxide pressure in the carbon dioxide delivery pipe 2 is about 1.7 kPa.

[0120] Specifically, the thermal mass flow meter 5 is composed of a power supply, a reading application and a set value controller and a metal sealed thermal mass flow controller, which regulates the gas flow to achieve the best mixing ratio of air and carbon dioxide gas for algae cultivation.

[0121] Specifically, the mixed gas is passed through a needle filter into the culture bottle 16, so that the pressure of the partial pressure valve 8 entering each culture bottle 16 is stabilized at about 0.2 kPa.

[0122] Specifically, pay attention to the total pressure of the pressure regulating valve 3. When increasing or decreasing the number of culture bottles 16, the total pressure of the pressure regulating valve 3 must be changed accordingly to avoid excessive or insufficient pressure.

[0123] Specifically, in the sampling device 1 - 6 , if algae liquid is to be extracted, the second water stop valve 21 is removed, and the algae liquid will flow into the sampling bottle 13 along the pipeline.

[0124] Specifically, the air intake pipe and the liquid supply pipe 15 are connected via a bridge pipe 14 , and a first water stop valve 20 is provided on the bridge pipe 14 .

[0125] If an emergency such as insufficient air pressure occurs, the algae may flow back into the gas supply device 1 - 1 . In this case, the first water stop valve 20 should be opened immediately to allow the algae to flow into the cultivation bottle 16 again along the liquid supply pipe 15 .

[0126] Specifically, the infusion tube in the flow control valve is a consumable. If a small number of bubbles are observed flowing in the tube, the infusion tube should be replaced.

[0127] Obviously, the specific implementation of the present invention is not limited to the above-mentioned methods. As long as various non-substantial improvements are made using the method concept and technical solution of the present invention, they are all within the scope of protection of the present invention.

Claims

1. A chemostat system for algae cultivation, characterized in that: It includes a gas delivery device, a cultivation device, a liquid delivery device, and a collection device; The cultivation device includes a cultivation bottle; the cultivation bottle is placed next to simulated sunlight, and the algae can use the light energy to convert carbon dioxide and water into organic matter for their own growth; the cultivation bottle is connected to a magnetic stirrer; The gas delivery device includes a gas supply component; The gas supply component includes an air supply component and a carbon dioxide supply component connected to the culture bottle; The air supply component and the carbon dioxide supply component are arranged in parallel; The infusion device includes a nutrient solution storage barrel; the nutrient solution storage barrel stores nutrient solution; the nutrient solution storage barrel is connected to the culture bottle; The collecting device includes a collecting bottle; The gas delivery device, the liquid delivery device, and the collection device are all connected to the culture bottle via a pipeline device; The chemostat system further comprises a sampling device; the sampling device comprises a sampling bottle; the pipeline device comprises a sampling pipeline, the sampling bottle is connected to the culture bottle through the sampling pipeline; the sampling pipeline is connected to a syringe.

2. The chemostat system for algae cultivation according to claim 1, characterized in that: The pipeline device includes an air delivery pipe and a carbon dioxide delivery pipe; The air delivery pipe and the carbon dioxide delivery pipe are both provided with a pressure regulating valve and a thermal mass flow meter.

3. The chemostat system for algae cultivation according to claim 1, characterized in that: The amount of carbon dioxide gas supplied into the culture bottle by the carbon dioxide supply component is greater than the amount of air gas supplied into the culture bottle by the air supply component.

4. The chemostat system for algae cultivation according to claim 1, characterized in that: The culture bottle is provided with a reserved pipeline device, and the reserved pipeline device includes a shower-type air outlet pipe arranged on the culture bottle.

5. The chemostat system for algae cultivation according to claim 1, characterized in that: The pipeline device also includes an air supply pipeline and a liquid supply pipeline; the air supply component is connected to the culture bottle through the air supply pipeline, and the nutrient solution storage barrel is connected to the culture bottle through the liquid supply pipeline; the air supply pipeline is also connected to the liquid supply pipeline through a bridging pipeline; the bridging pipeline is provided with a first water stop valve.

6. The chemostat system for algae cultivation according to claim 5, characterized in that: The gas delivery device further comprises an air humidifier, and the gas supply component is connected to the culture bottle via the air humidifier.

7. The chemostat system for algae cultivation according to claim 6, characterized in that: The gas delivery device further comprises a pressure-dividing valve; the air humidifier is connected to the culture bottle via the pressure-dividing valve.

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