Heating, stirring and liquid injection integrated device for nucleic acid extraction

Abstract

The utility model discloses a heating, stirring and liquid injection integrated device for nucleic acid extraction, which comprises an assembly support frame, the upper side of the assembly support frame is fixedly connected with a collection tube bottom plate mechanism, the upper side of the collection tube bottom plate mechanism is fixedly connected with a collection tube frame mechanism, the upper side of the collection tube frame mechanism is provided with a stirring assembly, and the stirring assembly is fixedly connected with the collection tube bottom plate mechanism. A pressing plate assembly is installed on the outer side of the stirring assembly, a baffle assembly is installed on the outer side of the pressing plate assembly, a drag chain is installed on the rear side of the collecting pipe frame mechanism, and a liquid adding mechanism is fixedly connected to the inner wall of the left assembly supporting frame. The peristaltic pump mounting metal plate is fixedly connected to the inner wall of the left side assembly supporting frame, the peristaltic pump is fixedly connected to the right side of the peristaltic pump mounting metal plate, and the cleaning agent bottle is mounted on the front side of the peristaltic pump. By means of the structure, the automatic liquid adding device can be used for automatically adding various reagents, heating is carried out while stirring is carried out, and the proper temperature is kept.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction devices, in particular to a heating, stirring and liquid injection integrated device for nucleic acid extraction. Background Art

[0002] Nucleic acid testing is a laboratory test that determines the presence of viral infection by detecting the genetic material (such as RNA or DNA) of a specific virus in a biological sample. This technology is widely used in many fields, including infectious diseases and contagious diseases, respiratory diseases, biology, animal husbandry and veterinary medicine, pediatrics, etc.

[0003] Before nucleic acid testing, nucleic acid extraction is required. Existing nucleic acid extraction devices cannot maintain temperature during stirring, and manual liquid addition is required after stirring, wasting time. Therefore, we propose an integrated heating, stirring, and liquid injection device for nucleic acid extraction to address this issue. Utility Model Content

[0004] The utility model aims to provide an integrated device for heating, stirring and liquid injection for nucleic acid extraction. The liquid can be drawn from a cleaning agent bottle by a peristaltic pump through a liquid adding mechanism. The liquid is switched through a 16-position rotary valve, flows from a joint through a steel needle and a stirring rod, and is then injected into a collection tube. The stirring assembly is driven by a motor and positioned by a guide rail, and can move up and down. The stirring rod rotates smoothly by upper and lower bearings. Synchronous wheels are fixed to the stirring rod and connected one by one by a synchronous belt. The stirring motor drives the stirring rod to rotate synchronously. The stirring assembly descends into the collection tube and cooperates with the tooth shape of the collection tube to perform rotational mixing. A guide groove is installed on the bottom plate of the collection tube and cooperates with the collection tube guide rail for guidance. When advancing, it rests on the upper limit position post. The collection tube rack in the collection tube rack mechanism has 16 positions and is engraved with numbers 1-16 on the surface. The bottom of the collection tube is pushed in and pulled out stably by the guide rail. The bottom of the collection tube is provided with 8 heating tubes that can heat the temperature from room temperature to 100 degrees. Stable temperature maintenance is achieved by temperature sensor control.

[0005] To achieve the above-mentioned objectives, a heating, stirring and liquid injection integrated device for nucleic acid extraction is provided, comprising a component support frame, a collection tube bottom plate mechanism is fixedly connected to the upper side of the component support frame, a collection tube rack mechanism is fixedly connected to the upper side of the collection tube bottom plate mechanism, a stirring component is installed on the upper side of the collection tube rack mechanism, a pressure plate assembly is installed on the outside of the stirring assembly, a baffle assembly is installed on the outside of the pressure plate assembly, a drag chain is installed on the rear side of the collection tube rack mechanism, and a liquid adding mechanism is fixedly connected to the inner wall of the left component support frame.

[0006] Preferably, the liquid adding mechanism includes a peristaltic pump mounting sheet metal, a peristaltic pump, a cleaning agent bottle, a 16-position rotary valve, and a 16-position rotary valve mounting sheet metal. The peristaltic pump mounting sheet metal is fixedly connected to the inner wall of the left component support frame, the peristaltic pump is fixedly connected to the right side of the peristaltic pump mounting sheet metal, the cleaning agent bottle is installed on the front side of the peristaltic pump, the 16-position rotary valve mounting sheet metal is installed on the left side of the cleaning agent bottle, and the 16-position rotary valve is fixedly connected to the upper side of the inner part of the 16-position rotary valve mounting sheet metal.

[0007] Preferably, the stirring assembly includes a stirring motor, a stirring motor mounting seat, a main drive synchronous belt, a stirring rod upper bearing, a stirring rod lower bearing, a stirring rod upper bearing mounting plate, a stirring rod synchronous wheel, a stirring lifting induction plate, a stirring lifting limit rod, a stirring rod lower bearing mounting plate, a stirring rod, and a stirring rod synchronous belt. The stirring motor mounting seat is fixedly connected to the left end of the stirring rod upper bearing mounting plate, the stirring motor is fixedly connected to the upper side of the stirring motor mounting seat, the main drive synchronous belt is installed at the output end of the stirring motor, the stirring rod synchronous wheel is installed at the right end of the main drive synchronous belt, the stirring rod upper bearing is installed on the upper side of the stirring rod synchronous wheel, the stirring rod synchronous belt is installed on the outer wall of the stirring rod synchronous wheel, and the stirring rod lower bearing is installed on the lower side of the stirring rod synchronous wheel.

[0008] Preferably, the upper bearing mounting plate of the stirring rod is installed on the outside of the upper bearing of the stirring rod, the lower bearing mounting plate of the stirring rod is installed on the outside of the lower bearing of the stirring rod, the stirring rod is installed on the lower side of the stirring rod synchronous wheel, the stirring lifting sensor plate is installed on the front and rear edges of the upper side of the lower bearing mounting plate of the stirring rod, and the stirring lifting limit rod is installed on the upper side of the lower bearing mounting plate of the stirring rod.

[0009] Preferably, the collection tube bottom plate mechanism includes a collection tube rack bottom plate, a collection tube guide groove, a collection tube rack in-place lock, a collection tube rack push sensor mounting block, a collection tube rack in-place sensor, and a collection tube rack pull-out limit position. The collection tube rack bottom plate is fixedly connected to the upper side of the component support frame, the collection tube guide groove is installed on the upper side of the collection tube rack bottom plate, the collection tube rack in-place lock is installed on the left front side of the collection tube rack bottom plate, the collection tube rack push sensor mounting block is installed in the middle of the left upper side of the collection tube rack bottom plate, the collection tube rack in-place sensor is installed on the upper side of the collection tube rack push sensor mounting block, and the collection tube rack pull-out limit position is installed on the upper side of the collection tube rack bottom plate.

[0010] Preferably, the collection tube rack mechanism includes a collection tube temperature control sensor, a collection tube rack side mounting plate, a collection tube rack body, a collection tube rack shell, a heating tube, and a collection tube rack bottom guide rail. The collection tube rack bottom guide rail is installed on the front and rear sides of the upper side of the collection tube rack bottom plate, the collection tube rack side mounting plate is installed on the upper side of the collection tube rack bottom guide rail, the collection tube rack shell is installed on the upper side of the collection tube rack side mounting plate, the collection tube rack body is installed inside the collection tube rack shell, and the collection tube temperature control sensor is installed on the upper left end of the collection tube rack body.

[0011] Preferably, the pressure plate assembly includes a stirring origin sensor, an origin sensor mounting plate sheet metal, a collection tube pressure plate mounting vertical plate, a lifting guide rail, a pressure plate mounting block, a pressure plate, and a pressure plate origin sensor. The collection tube pressure plate mounting vertical plate is installed on the front edge of the collection tube rack bottom plate, the origin sensor mounting plate sheet metal is installed on the outside of the two collection tube pressure plate mounting vertical plates, the stirring origin sensor is installed on the origin sensor mounting plate sheet metal, the pressure plate origin sensor is installed on the origin sensor mounting plate sheet metal in the middle position, the lifting guide rail is installed in the middle groove of the collection tube pressure plate mounting vertical plate, the pressure plate mounting block is installed in the middle of the front side of the collection tube pressure plate mounting vertical plate, and the pressure plate is installed on the pressure plate mounting block.

[0012] Preferably, the baffle assembly includes a pipe joint, a pipe joint mounting plate, a steel needle, a front baffle, a rear baffle, a stirring assembly top plate, a pressure plate upper and lower drive motor, and a stirring lifting motor. The front baffle and the rear baffle are respectively installed on the front and rear sides of the collection tube rack bottom plate, the stirring assembly top plate is installed in the middle of the upper side of the front baffle and the rear baffle, the pipe joint mounting plate is installed in the middle groove of the stirring assembly top plate, the stirring lifting motor is installed in the left and right circular holes on the top plate of the stirring assembly, the pressure plate upper and lower drive motor is installed in the front circular hole of the stirring assembly top plate, the pipe joint is installed in the groove of the pipe joint mounting plate, and the steel needle is installed on the lower side of the pipe joint.

[0013] The beneficial effects of the utility model are as follows: the liquid can be extracted from the cleaning agent bottle by the peristaltic pump through the liquid adding mechanism, switched through the 16-position rotary valve, and flows from the joint through the steel needle and the stirring rod to be filled into the collection tube; the stirring assembly is driven by a motor and positioned by a guide rail, and can move up and down; the stirring rod is smoothly rotated by the upper and lower bearings; a synchronous wheel is fixed on the stirring rod, which is connected by a synchronous belt one by one and driven by the stirring motor to realize synchronous rotation of the stirring rod; the stirring assembly descends into the collection tube and cooperates with the tooth shape of the collection tube to rotate and mix; a guide groove is installed on the bottom plate of the collection tube, and the collection tube guide rail is used for guidance, and when advancing, it is close to the limit position top column; the collection tube rack in the collection tube rack mechanism is provided with 16 positions, and there are 1-16 serial numbers engraved on the surface; the bottom of the collection tube is pushed in and pulled out stably by the guide rail; there are 8 heating tubes at the bottom of the collection tube, which can be heated from room temperature to 100 degrees, and stable temperature maintenance is achieved by temperature sensor control.

[0014] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0016] Figure 1 This is a front perspective view of a heating, stirring, and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0017] Figure 2 This is a rear perspective view of a heating, stirring, and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0018] Figure 3 This is a schematic diagram of the structure of a stirring component of a heating, stirring and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0019] Figure 4 This is a schematic diagram of the structure of a collection tube rack mechanism of a heating, stirring and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0020] Figure 5 This is a schematic structural diagram of a pressure plate assembly of a heating, stirring, and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0021] Figure 6 This is a schematic structural diagram of a baffle assembly of a heating, stirring, and liquid injection integrated device for nucleic acid extraction according to the present invention;

[0022] Figure 7This is a schematic diagram of the structure of the collection tube bottom plate mechanism of the utility model of a heating, stirring and liquid injection integrated device for nucleic acid extraction;

[0023] Legend:

[0024] 1. Component support frame; 2. Liquid adding mechanism; 201. Peristaltic pump mounting plate; 202. Peristaltic pump; 203. Detergent bottle; 204. 16-position rotary valve; 205. 16-position rotary valve mounting plate; 3. Stirring assembly; 301. Stirring motor; 302. Stirring motor mounting seat; 303. Main drive timing belt; 304. Stirring rod upper bearing; 305. Stirring rod lower bearing; 306. Stirring rod upper bearing mounting plate; 307. Stirring rod timing wheel; 308. Stirring lift sensor; 309. Stirring lift limit rod; 310. Stirring rod lower bearing mounting plate; 311. Stirring rod; 312. Stirring rod timing belt; 4. Collection tube rack mechanism; 401. Collection tube temperature control sensor; 402. Collection tube rack side mounting plate; 403. Collection tube rack body; 404. Collection tube rack housing; 405. Heating tube ;406, collection tube rack bottom guide rail; 5, pressure plate assembly; 501, stirring origin sensor; 502, origin sensor mounting plate sheet metal; 503, collection tube pressure plate mounting vertical plate; 504, lifting guide rail; 505, pressure plate mounting block; 506, pressure plate; 507, pressure plate origin sensor; 6, baffle assembly; 601, pipe joint; 602, pipe joint mounting plate; 603, steel needle; 604, front baffle; 605, rear baffle; 606, stirring assembly top plate; 607, pressure plate upper and lower drive motor; 608, stirring lifting motor; 7, drag chain; 8, collection tube bottom plate mechanism; 801, collection tube rack bottom plate; 802, collection tube guide groove; 803, collection tube rack in place lock; 804, collection tube rack push sensor mounting block; 805, collection tube rack in place sensor; 806, collection tube rack pull out limit position. DETAILED DESCRIPTION

[0025] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0026] Example:

[0027] Reference Figures 1 to 7The embodiment of the utility model is a heating, stirring and liquid injection integrated device for nucleic acid extraction, which includes a component support frame 1, a collection tube bottom plate mechanism 8 is fixedly connected to the upper side of the component support frame 1, a collection tube rack mechanism 4 is fixedly connected to the upper side of the collection tube bottom plate mechanism 8, a stirring component 3 is installed on the upper side of the collection tube rack mechanism 4, a pressure plate component 5 is installed on the outside of the stirring component 3, a baffle component 6 is installed on the outside of the pressure plate component 5, a drag chain 7 is installed on the rear side of the collection tube rack mechanism 4, and a liquid adding mechanism 2 is fixedly connected to the inner wall of the left component support frame 1.

[0028] The liquid adding mechanism 2 includes a peristaltic pump mounting sheet metal 201, a peristaltic pump 202, a cleaning agent bottle 203, a 16-position rotary valve 204, and a 16-position rotary valve mounting sheet metal 205. The peristaltic pump mounting sheet metal 201 is fixedly connected to the inner wall of the left component support frame 1, the peristaltic pump 202 is fixedly connected to the right side of the peristaltic pump mounting sheet metal 201, the cleaning agent bottle 203 is mounted on the front side of the peristaltic pump 202, the 16-position rotary valve mounting sheet metal 205 is mounted on the left side of the cleaning agent bottle 203, and the 16-position rotary valve 204 is fixedly connected to the upper side of the inner portion of the 16-position rotary valve mounting sheet metal 205. Liquid is drawn from the cleaning agent bottle 203 by the peristaltic pump 202, switched through the 16-position rotary valve 204, and flows from the No. 1-No. 16 connectors through the steel needle 603 and the stirring rod 311 before being added to the collection tube.

[0029] The stirring assembly 3 includes a stirring motor 301, a stirring motor mounting base 302, a main drive synchronous belt 303, a stirring rod upper bearing 304, a stirring rod lower bearing 305, a stirring rod upper bearing mounting plate 306, a stirring rod synchronous pulley 307, a stirring lifting induction plate 308, a stirring lifting limit rod 309, a stirring rod lower bearing mounting plate 310, a stirring rod 311, and a stirring rod synchronous belt 312. The stirring motor mounting base 302 is fixedly connected to the left end of the stirring rod upper bearing mounting plate 306, the stirring motor 301 is fixedly connected to the upper side of the stirring motor mounting base 302, the main drive synchronous belt 303 is installed at the output end of the stirring motor 301, the stirring rod synchronous pulley 307 is installed at the right end of the main drive synchronous belt 303, the stirring rod upper bearing 304 is installed on the upper side of the stirring rod synchronous pulley 307, the stirring rod synchronous belt 312 is installed on the outer wall of the stirring rod synchronous pulley 307, and the stirring rod lower bearing 305 is installed on the lower side of the stirring rod synchronous pulley 307. The stirring rod upper bearing mounting plate 306 is mounted on the outside of the stirring rod upper bearing 304, the stirring rod lower bearing mounting plate 310 is mounted on the outside of the stirring rod lower bearing 305, the stirring rod 311 is mounted on the underside of the stirring rod synchronous wheel 307, the stirring lift sensor 308 is mounted on the front and rear edges of the upper side of the stirring rod lower bearing mounting plate 310, and the stirring lift limit rod 309 is mounted on the upper side of the stirring rod lower bearing mounting plate 310. The stirring assembly 3 is driven by a motor and positioned on a guide rail, and can move up and down. The stirring rod 311 rotates smoothly thanks to the upper and lower bearings. By fixing the synchronous wheel on the stirring rod 311, connected one by one by a synchronous belt, and driven by the stirring motor 301, the 16 stirring rods 311 rotate synchronously. The stirring assembly 3 is lowered into the collection tube and mates with the collection tube thread shape to rotate and mix.

[0030] The collection tube bottom plate mechanism 8 includes a collection tube rack bottom plate 801, a collection tube guide groove 802, a collection tube rack in-place lock 803, a collection tube rack push sensor mounting block 804, a collection tube rack in-place sensor 805, and a collection tube rack pull-out limit position 806. The collection tube rack bottom plate 801 is fixedly connected to the upper side of the component support frame 1, the collection tube guide groove 802 is installed on the upper side of the collection tube rack bottom plate 801, the collection tube rack in-place lock 803 is installed on the front left side of the collection tube rack bottom plate 801, the collection tube rack push sensor mounting block 804 is installed in the middle of the upper left side of the collection tube rack bottom plate 801, the collection tube rack in-place sensor 805 is installed on the upper side of the collection tube rack push sensor mounting block 804, and the collection tube rack pull-out limit position 806 is installed on the upper side of the collection tube rack bottom plate 801. A guide groove is installed on the bottom plate 801 of the collection tube rack, which cooperates with the collection tube guide rail for guidance. When pushed forward, it leans against the limit position top column, and the collection tube rack is fixed with the collection tube rack lock 903 to prevent vibration deviation. A position sensor is provided, and an electrical signal feedback is generated after the collection tube rack is pushed into position to realize automatic sensing.

[0031] The collection tube rack mechanism 4 includes a collection tube temperature control sensor 401, a collection tube rack side mounting plate 402, a collection tube rack body 403, a collection tube rack housing 404, a heating tube 405, and a collection tube rack bottom rail 406. The collection tube rack bottom rail 406 is mounted on the front and rear sides of the collection tube rack base plate 801. The collection tube rack side mounting plate 402 is mounted above the collection tube rack bottom rail 406. The collection tube rack housing 404 is mounted above the collection tube rack side mounting plate 402. The collection tube rack body 403 is mounted inside the collection tube rack housing 404. The collection tube temperature control sensor 401 is mounted on the upper left end of the collection tube rack body 403. The collection tube rack has 16 positions, numbered 1 to 16 on the surface. The bottom of the collection tube is supported by a guide rail for stable movement in and out. Eight heating tubes 405 are installed at the bottom of the collection tube, capable of heating the tube from room temperature to 100 degrees Celsius. Stable temperature maintenance is achieved through temperature sensor control.

[0032] The pressure plate assembly 5 includes a stirring origin sensor 501, an origin sensor mounting plate sheet metal 502, a collection tube pressure plate mounting vertical plate 503, a lifting guide rail 504, a pressure plate mounting block 505, a pressure plate 506, and a pressure plate origin sensor 507. The collection tube pressure plate mounting vertical plate 503 is installed on the front edge of the collection tube rack bottom plate 801, the origin sensor mounting plate sheet metal 502 is installed on the outside of the two collection tube pressure plate mounting vertical plates 503, the stirring origin sensor 501 is installed on the origin sensor mounting plate sheet metal 502, the pressure plate origin sensor 507 is installed on the origin sensor mounting plate sheet metal 502 in the middle position, the lifting guide rail 504 is installed in the middle groove of the collection tube pressure plate mounting vertical plate 503, the pressure plate mounting block 505 is installed in the middle of the front side of the collection tube pressure plate mounting vertical plate 503, and the pressure plate 506 is installed on the pressure plate mounting block 505. The collection tube pressing plate 506 is positioned by a guide rail and is driven by a motor to move up and down to press the collection tube to prevent the collection tube from jumping during stirring and ensure the stability of the collection tube during stirring and adding liquid. A pressing plate origin sensor 507 is provided to realize signal feedback during automatic operation.

[0033] The baffle assembly 6 includes a pipe joint 601, a pipe joint mounting plate 602, a steel needle 603, a front baffle 604, a rear baffle 605, a stirring assembly top plate 606, a pressure plate up and down drive motor 607, and a stirring lifting motor 608. The front baffle 604 and the rear baffle 605 are respectively installed on the front and back sides of the collection tube rack bottom plate 801, the stirring assembly top plate 606 is installed in the middle of the upper side of the front baffle 604 and the rear baffle 605, the pipe joint mounting plate 602 is installed in the middle groove of the stirring assembly top plate 606, the stirring lifting motor 608 is installed in the left and right circular holes of the stirring assembly top plate 606, the pressure plate up and down drive motor 607 is installed in the front circular hole of the stirring assembly top plate 606, the pipe joint 601 is installed in the groove of the pipe joint mounting plate 602, and the steel needle 603 is installed on the lower side of the pipe joint 601. The stirring rod 311 is a hollow structure, with a pipe joint 601 and a steel needle 603 on the top that penetrate into the stirring rod 311, so that liquid can be added to the collection tube. The pressure plate up and down drive motor 607 and the stirring lifting motor 608 control the stirring component 3 to move up and down.

[0034] Working Principle: During operation, liquid is pumped from the detergent bottle 203 via the peristaltic pump 202. The liquid is switched through the 16-position rotary valve 204, flowing from connectors 1-16 through the steel needle 603 and stirring rod 311, ultimately filling the collection tube. The stirring assembly 3 then moves up and down, driven by the upper and lower drive motors 607 and the stirring lift motor 608. The stirring rods 311 rotate smoothly via upper and lower bearings. Synchronous pulleys are fixed to the stirring rods 311, connected one by one via a synchronous belt. Driven by the stirring motor 301, all 16 stirring rods 311 rotate synchronously. The stirring assembly 3 descends into the collection tube, engaging the tube's threaded shape and performing rotational mixing. Guide grooves are installed on the collection tube rack base plate 801, aligning with the collection tube's guide rails. During advancement, the rack rests against the limit stop post. The rack's locking latch 903 prevents vibration deflection. A position sensor provides electrical feedback when the rack is in position, enabling automated sensing. The collection tube holder has 16 positions, with numbers 1 to 16 engraved on the surface. The bottom of the collection tube is supported by a guide rail for stable movement, allowing it to be pushed in and pulled out. Eight heating tubes 405 are located at the bottom of the collection tube, capable of heating from room temperature to 100 degrees Celsius. A temperature sensor controls the temperature to maintain a stable state. The collection tube pressure plate 506 is positioned by a guide rail and is driven up and down by a motor, pressing down on the collection tube to prevent it from bouncing during stirring and ensuring stability during stirring and liquid addition. A pressure plate origin sensor 507 is provided for signal feedback during automated operation. The stirring rod 311 is a hollow structure, with a pipe joint 601 and a steel needle 603 inserted into the rod 311 to allow liquid to be added to the collection tube. The pressure plate's up and down drive motor 607 and the stirring lift motor 608 control the up and down movement of the stirring assembly 3.

[0035] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A heating, stirring and liquid injection integrated device for nucleic acid extraction, comprising a component support frame (1), characterized in that: The upper side of the component support frame (1) is fixedly connected to a collection tube bottom plate mechanism (8), the upper side of the collection tube bottom plate mechanism (8) is fixedly connected to a collection tube rack mechanism (4), the upper side of the collection tube rack mechanism (4) is installed with a stirring component (3), the outer side of the stirring component (3) is installed with a pressure plate component (5), the outer side of the pressure plate component (5) is installed with a baffle component (6), the rear side of the collection tube rack mechanism (4) is installed with a drag chain (7), and the inner wall of the left component support frame (1) is fixedly connected to a liquid adding mechanism (2).

2. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 1, characterized in that: The liquid adding mechanism (2) comprises a peristaltic pump mounting sheet metal (201), a peristaltic pump (202), a cleaning agent bottle (203), a 16-position rotary valve (204), and a 16-position rotary valve mounting sheet metal (205), wherein the peristaltic pump mounting sheet metal (201) is fixedly connected to the inner wall of the left component support frame (1), the peristaltic pump (202) is fixedly connected to the right side of the peristaltic pump mounting sheet metal (201), the cleaning agent bottle (203) is mounted on the front side of the peristaltic pump (202), the 16-position rotary valve mounting sheet metal (205) is mounted on the left side of the cleaning agent bottle (203), and the 16-position rotary valve (204) is fixedly connected to the upper inner side of the 16-position rotary valve mounting sheet metal (205).

3. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 1, characterized in that: The stirring assembly (3) comprises a stirring motor (301), a stirring motor mounting seat (302), a main drive synchronous belt (303), a stirring rod upper bearing (304), a stirring rod lower bearing (305), a stirring rod upper bearing mounting plate (306), a stirring rod synchronous wheel (307), a stirring lifting induction plate (308), a stirring lifting limit rod (309), a stirring rod lower bearing mounting plate (310), a stirring rod (311), and a stirring rod synchronous belt (312). The stirring motor mounting seat (302) is fixedly connected to the stirring rod upper bearing mounting plate ( 306), the stirring motor (301) is fixedly connected to the upper side of the stirring motor mounting seat (302), the main drive synchronous belt (303) is installed at the output end of the stirring motor (301), the stirring rod synchronous wheel (307) is installed at the right end of the main drive synchronous belt (303), the stirring rod upper bearing (304) is installed on the upper side of the stirring rod synchronous wheel (307), the stirring rod synchronous belt (312) is installed on the outer wall of the stirring rod synchronous wheel (307), and the stirring rod lower bearing (305) is installed on the lower side of the stirring rod synchronous wheel (307).

4. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 3, characterized in that: The stirring rod upper bearing mounting plate (306) is mounted on the outside of the stirring rod upper bearing (304), the stirring rod lower bearing mounting plate (310) is mounted on the outside of the stirring rod lower bearing (305), the stirring rod (311) is mounted on the lower side of the stirring rod synchronous wheel (307), the stirring lifting sensor plate (308) is mounted on the front and rear edges of the upper side of the stirring rod lower bearing mounting plate (310), and the stirring lifting limit rod (309) is mounted on the upper side of the stirring rod lower bearing mounting plate (310).

5. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 1, characterized in that: The collection tube bottom plate mechanism (8) includes a collection tube rack bottom plate (801), a collection tube guide groove (802), a collection tube rack in-place lock (803), a collection tube rack push sensor mounting block (804), a collection tube rack in-place sensor (805), and a collection tube rack pull-out limit position (806). The collection tube rack bottom plate (801) is fixedly connected to the upper side of the component support frame (1), the collection tube guide groove (802) is mounted on the upper side of the collection tube rack bottom plate (801), the collection tube rack in-place lock (803) is mounted on the left front side of the collection tube rack bottom plate (801), the collection tube rack push sensor mounting block (804) is mounted in the middle of the left upper side of the collection tube rack bottom plate (801), the collection tube rack in-place sensor (805) is mounted on the upper side of the collection tube rack push sensor mounting block (804), and the collection tube rack pull-out limit position (806) is mounted on the upper side of the collection tube rack bottom plate (801).

6. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 5, characterized in that: The collection tube rack mechanism (4) includes a collection tube temperature control sensor (401), a collection tube rack side mounting plate (402), a collection tube rack body (403), a collection tube rack shell (404), a heating tube (405), and a collection tube rack bottom guide rail (406). The collection tube rack bottom guide rail (406) is installed on the front and rear sides of the upper side of the collection tube rack bottom plate (801). The collection tube rack side mounting plate (402) is installed on the upper side of the collection tube rack bottom guide rail (406). The collection tube rack shell (404) is installed on the upper side of the collection tube rack side mounting plate (402). The collection tube rack body (403) is installed inside the collection tube rack shell (404). The collection tube temperature control sensor (401) is installed on the upper left side of the collection tube rack body (403).

7. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 5, characterized in that: The pressure plate assembly (5) includes a stirring origin sensor (501), an origin sensor mounting plate sheet metal (502), a collection tube pressure plate mounting vertical plate (503), a lifting guide rail (504), a pressure plate mounting block (505), a pressure plate (506), and a pressure plate origin sensor (507). The collection tube pressure plate mounting vertical plate (503) is mounted on the front edge of the collection tube rack bottom plate (801). The origin sensor mounting plate sheet metal (502) is mounted on the two collection tube pressure plate mounting vertical plates (503). ), the stirring origin sensor (501) is mounted on the origin sensor mounting plate sheet metal (502), the pressure plate origin sensor (507) is mounted on the origin sensor mounting plate sheet metal (502) in the middle position, the lifting guide rail (504) is mounted in the middle groove of the collection tube pressure plate mounting vertical plate (503), the pressure plate mounting block (505) is mounted in the middle of the front side of the collection tube pressure plate mounting vertical plate (503), and the pressure plate (506) is mounted on the pressure plate mounting block (505).

8. The integrated device for heating, stirring and liquid injection for nucleic acid extraction according to claim 5, characterized in that: The baffle assembly (6) includes a pipe joint (601), a pipe joint mounting plate (602), a steel needle (603), a front baffle (604), a rear baffle (605), a stirring assembly top plate (606), a pressing plate upper and lower drive motor (607), and a stirring lifting motor (608). The front baffle (604) and the rear baffle (605) are respectively mounted on the front and rear sides of the collection tube rack bottom plate (801). The stirring assembly top plate (606) is mounted between the front baffle (604) and the rear baffle. The pipe joint mounting plate (602) is mounted in the middle of the upper side of the stirring assembly top plate (606), the stirring lifting motor (608) is mounted in the circular holes on the left and right sides of the stirring assembly top plate (606), the pressing plate upper and lower driving motor (607) is mounted in the circular hole on the front side of the stirring assembly top plate (606), the pipe joint (601) is mounted in the groove of the pipe joint mounting plate (602), and the steel needle (603) is mounted on the lower side of the pipe joint (601).

Images