Novel cell culture medium bottle

Abstract

According to the novel cell culture medium bottle disclosed by the utility model, through the arrangement of the ventilation transfer structure, when a sealing plug is opened and ventilation is carried out on the inside of the bottle body through a communicating pipe, entering air is treated firstly, so that external air is prevented from directly entering and polluting a culture medium; through the arrangement of the stirring mounting structure, the lower end of the vertical shaft body can still be located in the buckling ring in the rotating and disassembling process of the top cover, the vertical shaft body can be driven to rotate when the top cover rotates, the culture medium is stirred through a stirring rod, and sediment is avoided when the culture medium is poured and used.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture medium, in particular to a novel cell culture medium bottle. Background Art

[0002] Cell engineering is a type of bioengineering, and cell culture is inseparable from cell culture medium. The so-called cell culture medium is not only the basic substance that provides cell nutrition and promotes cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. After the configuration is completed, the cell culture medium is usually placed in the bottle and poured out from the inside when it is used.

[0003] The existing culture medium bottle has a simple structure. When ventilation is required, the entry of outside air can easily contaminate the culture medium. At the same time, after being placed in the bottle for a long time, the culture medium itself will produce a certain amount of precipitation when pouring out during use, resulting in poor effect of the poured culture medium. Utility Model Content

[0004] In view of the problems existing in the prior art, the utility model discloses a novel cell culture medium bottle, which adopts the following technical solution: it includes a bottle body, an internal thread, a top cover, an external thread mounting frame, an air outlet pipe, a one-way valve, a connecting pipe, a sealing plug, a vertical shaft, a stirring rod, a ventilation transfer structure, a rectangular bottom mounting frame and a stirring mounting structure. The inner wall of the bottle body near the upper opening has an internal thread, and the top cover is mounted with the internal thread through the external thread mounting frame integrally formed at its lower part. An air outlet pipe is fixed on the top cover, and a one-way valve is provided on the air outlet pipe. The setting of the one-way valve can prevent external air from entering the bottle body from here. To prevent pollution, a connecting pipe is provided at the center above the top cover, which is connected to the ventilation transfer structure provided in the top cover and is closed by a detachable sealing plug. When ventilation is required, the sealing plug is pulled out, and external air is introduced through existing technologies such as a fan and filtered under the action of the ventilation transfer structure. A vertical axis is fixedly installed under the top cover through a stirring installation structure, and a number of stirring rods are evenly distributed on its side. When it needs to be poured for use, the top cover needs to be rotated, and the vertical axis and the stirring rods can be driven to rotate during the rotation process to avoid precipitation. A handle is fixed on the outer wall of the bottle body for easy removal of the bottle.

[0005] As a preferred technical solution of the present invention, the inner side wall of the bottle body has a cavity, and a number of rubber columns are fixed in the cavity to play a certain buffering role against external collisions.

[0006] As an optimal technical solution of the present invention, the ventilation transfer structure includes an upper ring, a lower ring, a sterilizing filter plate, a bottom sealing plate and a through hole. The horizontal plane where the top cover is connected to the external threaded mounting frame is the bottom sealing plate. The top cover above the bottom sealing plate is a cylindrical cavity. The upper ring and the lower ring are fixed on the inner wall of the cavity. The sterilizing filter plate is placed between the upper ring and the lower ring. Several through holes are opened on the bottom sealing plate. The lower end of the air outlet pipe is fixed on the bottom sealing plate and connected to the space below it. After the external air enters the top cover through the connecting pipe, it is sterilized and filtered by the sterilizing filter plate before entering the bottle body to avoid contamination of the culture medium.

[0007] As a preferred technical solution of the present invention, a buckle ring is fixed to the bottom surface of the bottle body. After the top cover is closed and installed, the lower end of the vertical shaft can be inserted into the buckle ring.

[0008] As an optimal technical solution of the present invention, the stirring installation structure includes a fixed shaft, a spring, a rectangular rod and a blind hole. A rectangular bottom mounting frame is fixed at the bottom center of the bottom sealing plate, and a fixed shaft is fixed at the inner center thereof. The spring is fixed on the top surface of the rectangular bottom mounting frame, and the whole is sleeved outside the fixed shaft. The rectangular rod is placed in the rectangular bottom mounting frame, and a blind hole is opened on the top thereof. It is buckled on the fixed shaft through the blind hole. The top surface of the rectangular rod is fixedly connected to the spring, and the bottom surface of the rectangular rod is fixed to the vertical shaft. The setting of the spring can ensure that the stirring rod can always be in a position to stir the culture medium during the rotation and lifting of the top cover.

[0009] The beneficial effects of the present invention are as follows: through the setting of the ventilation transfer structure, when the sealing plug is opened to ventilate the bottle body through the connecting tube, the incoming air is first processed to avoid direct entry of external air to contaminate the culture medium; through the setting of the stirring installation structure, the lower end of the vertical shaft can still be in the buckle ring during the rotation and disassembly of the top cover, and when the top cover rotates, it can drive the vertical shaft to rotate and use the stirring rod to stir the culture medium to avoid precipitation when pouring and using. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is a schematic diagram of the structure of the utility model;

[0011] Figure 2 This is a schematic diagram of the bottom-up structure of the present invention;

[0012] Figure 3 It is a schematic cross-sectional structural diagram of some components of the present invention.

[0013] In the figure: bottle body 1, handle 101, cavity 102, rubber column 103, internal thread 2, buckle ring 201, top cover 3, external thread mounting frame 4, air outlet pipe 5, one-way valve 6, connecting pipe 7, sealing plug 8, vertical shaft 9, stirring rod 10, upper ring 11, lower ring 12, sterilizing filter plate 13, bottom sealing plate 14, through hole 15, rectangular bottom mounting frame 16, fixed shaft 17, spring 18, rectangular rod 19, blind hole 20. DETAILED DESCRIPTION

[0014] Example 1

[0015] like Figures 1 to 3 As shown, the utility model discloses a novel cell culture medium bottle, which adopts the following technical solution: it includes a bottle body 1, an internal thread 2, a top cover 3, an external thread mounting frame 4, an air outlet pipe 5, a one-way valve 6, a connecting pipe 7, a sealing plug 8, a vertical shaft 9, a stirring rod 10, a ventilation transfer structure, a rectangular bottom mounting frame 16 and a stirring mounting structure. The inner wall of the bottle body 1 near the upper opening has an internal thread 2, and the top cover 3 is mounted with the internal thread 2 through the external thread mounting frame 4 integrally formed at its lower part. The air outlet pipe 5 is fixed on the top cover 3, and the air outlet pipe 5 is provided with a one-way valve 6. A connecting pipe 7 is provided at the center above the top cover 3, which is connected to the ventilation transfer structure provided in the top cover 3 and is closed by a detachable sealing plug 8. The vertical shaft 9 is fixedly installed below the top cover 3 through the stirring mounting structure, and a number of stirring rods 10 are evenly distributed on its side. A handle 101 is fixed on the outer wall of the bottle body 1.

[0016] As a preferred technical solution of the present invention, the inner side wall of the bottle body 1 has a cavity 102 , and a plurality of rubber columns 103 are fixed in the cavity 102 .

[0017] As an optimal technical solution of the present invention, in order to avoid contamination of the internal culture medium by external air during ventilation, a ventilation transfer structure is set, which specifically includes an upper ring 11, a lower ring 12, a sterilizing filter plate 13, a bottom sealing plate 14 and a through hole 15. The horizontal surface where the top cover 3 is connected to the external threaded mounting frame 4 is the bottom sealing plate 14. The top cover 3 above the bottom sealing plate 14 is a columnar cavity, and the upper ring 11 and the lower ring 12 are fixed on the inner wall of the cavity. The sterilizing filter plate 13 is placed between the upper ring 11 and the lower ring 12. A number of through holes 15 are opened on the bottom sealing plate 14, and the lower end of the air outlet pipe 5 is fixed on the bottom sealing plate 14 and is connected to the space below it.

[0018] As a preferred technical solution of the present invention, a buckle ring 201 is fixed to the inner bottom surface of the bottle body 1 .

[0019] As an optimal technical solution of the present invention, the specific setting method of the stirring installation structure is that a rectangular bottom mounting frame 16 is fixed at the bottom center of the bottom sealing plate 14, and a fixed shaft 17 is fixed at the inner center thereof. A spring 18 is fixed on the top surface of the inner part of the rectangular bottom mounting frame 16, and the whole is sleeved outside the fixed shaft 17. A rectangular rod 19 is placed in the rectangular bottom mounting frame 16, and a blind hole 20 is opened on the top thereof. It is buckled on the fixed shaft 17 through the blind hole 20. The top surface of the rectangular rod 19 is fixedly connected to the spring 18, and the bottom surface of the rectangular rod 19 is fixed to the vertical shaft 9.

[0020] The working principle of the present invention is as follows: when ventilation is required, air is supplied to the connecting pipe 7 through an external fan or other air supply device, and the external air is sent into the bottle body 1. When entering, it is sterilized and filtered through the sterilization filter plate 13, and then sent into the bottle body 1 to avoid direct entry of external air and contamination of the internal culture medium. When it is necessary to pour it for use, the top cover 3 is rotated, and during the rotation process, it can drive the rectangular bottom mounting frame 16 and the rectangular rod body 19 to rotate. The rotation of the rectangular rod body 19 drives the vertical shaft body 9 to rotate, and the stirring rod 10 is used to stir the culture medium to avoid precipitation when the top cover is opened for pouring. At the same time, the setting of the spring 18 enables the top cover to keep the bottom end of the vertical shaft body 9 always in the buckle ring 201 during the process of rotation and lifting.

[0021] Components not described in detail herein are prior art.

[0022] Although the specific embodiments of the present invention are described in detail above, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by ordinary technicians in this field, various changes can be made without departing from the purpose of the present invention. Modifications or deformations that do not involve creative work are still within the scope of protection of the present invention.

Claims

1. A novel cell culture medium bottle, characterized in that: The invention comprises a bottle body (1), an internal thread (2), a top cover (3), an external thread mounting frame (4), an air outlet pipe (5), a one-way valve (6), a connecting pipe (7), a sealing plug (8), a vertical shaft body (9), a stirring rod (10), a ventilation transfer structure, a rectangular bottom mounting frame (16) and a stirring mounting structure; the inner wall of the bottle body (1) near the upper opening is provided with an internal thread (2), and the top cover (3) is mounted in conjunction with the internal thread (2) through an external thread mounting frame (4) integrally formed at its lower part; an air outlet pipe (5) is fixed on the top cover (3), and a one-way valve (6) is provided on the air outlet pipe (5); a connecting pipe (7) is provided at the center of the upper part of the top cover (3), which is connected to the ventilation transfer structure provided in the top cover (3) and is sealed by a detachable sealing plug (8); a vertical shaft body (9) is fixedly mounted below the top cover (3) through the stirring mounting structure, and a plurality of stirring rods (10) are evenly distributed on its side.

2. A novel cell culture medium bottle according to claim 1, characterized in that: A handle (101) is fixed on the outer wall of the bottle body (1).

3. The novel cell culture medium bottle according to claim 1, characterized in that: The inner side wall of the bottle body (1) has a cavity (102), and a plurality of rubber columns (103) are fixed in the cavity (102).

4. The novel cell culture medium bottle according to claim 1, characterized in that: The ventilation transfer structure includes an upper ring (11), a lower ring (12), a sterilizing filter plate (13), a bottom sealing plate (14) and a through hole (15); the horizontal surface where the top cover (3) is connected to the external threaded mounting frame (4) is the bottom sealing plate (14), and the top cover (3) above the bottom sealing plate (14) is a columnar cavity, and the upper ring (11) and the lower ring (12) are fixed to the inner wall of the cavity; the sterilizing filter plate (13) is placed between the upper ring (11) and the lower ring (12); a plurality of through holes (15) are opened on the bottom sealing plate (14), and the lower end of the air outlet pipe (5) is fixed on the bottom sealing plate (14) and communicated with the space below it.

5. The novel cell culture medium bottle according to claim 1, characterized in that: A buckle ring (201) is fixed to the inner bottom surface of the bottle body (1).

6. The novel cell culture medium bottle according to claim 4, characterized in that: The stirring installation structure comprises a fixed shaft (17), a spring (18), a rectangular rod (19) and a blind hole (20); a rectangular bottom installation frame (16) is fixed at the bottom center of the bottom sealing plate (14), and a fixed shaft (17) is fixed at the inner center of the rectangular bottom installation frame (16); the spring (18) is fixed on the inner top surface of the rectangular bottom installation frame (16), and the spring (18) is integrally sleeved outside the fixed shaft (17); the rectangular rod (19) is placed in the rectangular bottom installation frame (16), and a blind hole (20) is opened on the top of the rectangular rod, and is buckled onto the fixed shaft (17) through the blind hole (20); the top surface of the rectangular rod (19) is fixedly connected to the spring (18); and the bottom surface of the rectangular rod (19) is fixed to the vertical shaft (9).

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