Freezing and grinding device for animal and plant samples

Abstract

The animal and plant sample freezing and grinding device comprises a shell, a liquid nitrogen storage pipe, a grinding assembly and a sealing cover, the upper end of the shell is open, and the side wall of the shell is provided with a liquid nitrogen inlet and a liquid nitrogen outlet; the liquid nitrogen storage tank is communicated with the liquid nitrogen inlet; the grinding assembly comprises a grinding cylinder, a grinding cylinder driving mechanism and a plurality of grinding columns, an opening in the upper end of the grinding cylinder is located in the shell, the outer wall of the grinding cylinder is rotationally connected with the inner wall of the shell, the grinding cylinder driving mechanism is fixedly installed on the outer wall of the shell, and the output end of the grinding cylinder driving mechanism is in transmission connection with the grinding cylinder; according to the liquid nitrogen freezing and grinding device, circulating liquid nitrogen is used for freezing a sample in the grinding cylinder, the grinding columns on the inner wall of the grinding cylinder are used for grinding the sample, and the liquid nitrogen freezing and grinding device has high grinding efficiency.

Description

Technical Field

[0001] The utility model belongs to the technical field of sample detection, and in particular relates to a freezing and grinding device for animal and plant samples. Background Art

[0002] In experiments related to tissue RNA extraction, it is often necessary to grind the tissue under low-temperature conditions to make it into powder before proceeding to the next experiment. For example, staff often use liquid nitrogen to quickly freeze animal and plant samples, and then use tools such as mortars to grind the samples. For example, Patent 202222681817.2 provides a tissue freezing and dosing grinding device, and Patent 202121761232.0 provides a freezing grinding device. However, due to the danger of liquid nitrogen and its rapid volatilization rate, the above two devices have low safety, and the direct manual grinding method also has low grinding efficiency. Therefore, it is necessary to improve the existing technology to improve safety and work efficiency. Utility Model Content

[0003] The purpose of the utility model is to provide a freezing and grinding device for animal and plant samples, aiming to solve the above-mentioned problems in the prior art.

[0004] In order to solve the above problems, the present invention adopts the following technical solutions:

[0005] A device for freezing and grinding animal and plant samples comprises a shell, a liquid nitrogen storage tank, a grinding assembly and a sealing cover, wherein the upper end of the shell is open, and the side walls thereof respectively extend outward to form a liquid nitrogen inlet and a liquid nitrogen outlet; the outlet end of the liquid nitrogen storage tank is connected to the liquid nitrogen inlet; the grinding assembly comprises a grinding cylinder, a grinding cylinder driving mechanism and a plurality of grinding posts, the upper end of the grinding cylinder being open and located inside the shell, the outer wall of the grinding cylinder being rotatably connected to the inner wall of the shell, the grinding cylinder driving mechanism being fixedly mounted on the outer wall of the shell, and the output end of the grinding cylinder passing through the outer wall of the shell being transmission-connected to the grinding cylinder, the plurality of grinding posts being parallel to the axial direction of the grinding cylinder and being fixedly connected to the inner wall of the grinding cylinder in sequence along the circumference of the inner wall of the grinding cylinder; and the sealing cover being detachably connected to the upper end of the shell.

[0006] The beneficial effects of the present invention are as follows: an independent shell and a grinding cylinder are provided, a cavity for liquid nitrogen circulation is formed between the shell and the grinding cylinder, the liquid nitrogen enters from the liquid nitrogen inlet on the shell and leaves through the liquid nitrogen outlet, and the animal and plant samples in the grinding cylinder are quickly frozen, and the liquid nitrogen outlet can be connected to other collection equipment to prevent the liquid nitrogen from escaping and ensure safety, the grinding cylinder is rotatably connected to the shell and is driven to rotate by the driving device, and can crush and grind the frozen sample under rotation in conjunction with the grinding column in the cylinder itself. Specifically, when the grinding cylinder starts to rotate, the frozen sample is thrown toward the inner wall of the grinding cylinder under the action of centrifugal force, and is gradually crushed and refined under the impact after contacting the grinding column. Compared with the grinding device in the prior art, the traditional rotating blade method is abolished and replaced with a grinding column directly fixed to the inner wall of the grinding cylinder, which greatly extends the service life of the device and does not require replacement of blades or rotating shafts. In addition, the presence of the sealing cover can also effectively prevent the leakage of volatile nitrogen and the splashing of ground powder out of the grinding cylinder.

[0007] Furthermore, the outer wall of each grinding column has a plurality of ridges along its circumference, and the number of the ridges is an even number and is at least 6.

[0008] A further beneficial effect of the present invention is that, by providing the ridges, the frozen sample is subjected to more concentrated force when it contacts the grinding column, which helps to quickly break it into small pieces and then gradually turn it into powder.

[0009] Furthermore, the grinding assembly also includes an electric cylinder, a connecting rod and a rolling head. The sealing cover has an assembly cavity, and the electric cylinder is installed in the assembly cavity. One end of the connecting rod passes through the lower end of the sealing cover and is transmission-connected to the movable end of the electric cylinder; one end of the rolling head is fixedly connected to the other end of the connecting rod, and the other end is hemispherical in shape to fit the bottom of the grinding cylinder, and a plurality of spherical bumps are detachably connected to its surface. The electric cylinder drives the rolling head to abut against the bottom of the grinding cylinder.

[0010] A further beneficial effect of the present invention is that a rolling head that can move up and down is additionally provided, and a protrusion is also provided on the rolling head. When the rolling head moves up and down with the connecting rod, the frozen sample at the bottom of the grinding cylinder is preliminarily crushed. The sample after the preliminary crushing is convenient for further refinement by the grinding column, and the spherical protrusion on the rolling head can increase the pressure of the rolling head on the sample, so that the rolling head can easily crush the frozen sample.

[0011] Furthermore, the grinding assembly also includes a hollow limiting column made of a thermal insulation plate, one end of the hollow limiting column is vertically and fixedly connected to the lower end of the sealing cover, and the outer wall of the connecting rod is slidably connected to the inner wall of the hollow limiting column.

[0012] Further beneficial effects of the present invention are: first, the outer wall of the hollow limit column can protect the connecting rod; secondly, the hollow limit column can play a guiding role, preventing the connecting rod from deviating when it moves up and down under the action of the electric cylinder, ensuring that the connecting rod is always in the same straight line with the axis of the grinding cylinder.

[0013] Furthermore, the bottom of the grinding cylinder is arc-shaped, and the plurality of protrusions are spirally distributed along the surface of the grinding cylinder from the lower end of the rolling head.

[0014] A further beneficial effect of the present invention is that the spirally distributed protrusions cooperate with the arc surface of the bottom of the grinding cylinder to ensure that the material accumulated at the bottom of the barrel is subjected to uniform force, thereby grinding the frozen sample in the grinding cylinder more finely.

[0015] Furthermore, the grinding cylinder driving mechanism includes a rotating motor, a reducer and a rotating support seat, the casing of the rotating motor is fixedly mounted on the bottom of the outer shell; the reducer is coaxially mounted on the motor pump of the rotating motor, the output shaft of the reducer vertically passes through the bottom of the outer shell and the shaft body is provided with a sealing ring, and is rotatably connected to the bottom of the outer shell; the lower end surface of the rotating support seat is rotatably connected to the inner bottom of the outer shell and fixedly connected to the output shaft of the reducer, and the upper end surface of the rotating support seat is fixedly connected to the outer bottom of the grinding cylinder.

[0016] A further beneficial effect of the present invention is that the rotation speed is adjusted by the rotating motor and the reducer, and the grinding drum is driven to rotate by the rotating support seat. Due to the supporting effect of the rotating support seat, the stability of the grinding drum can be guaranteed after it starts to rotate.

[0017] Furthermore, it includes a plurality of guide ring plates, which are coaxially fixed to the outer wall of the grinding cylinder in sequence along the axial direction of the grinding cylinder, and each of the guide ring plates is provided with a plurality of blades at intervals.

[0018] A further beneficial effect of the present invention is that the guide plate is designed to rotate together with the grinding cylinder, and the rotation of the guide plate drives the air flow of the shell, thereby assisting the liquid nitrogen to quickly fill the space inside the shell.

[0019] Furthermore, it also includes a fixed bearing and a plurality of annular sealing strips, the fixed bearing is installed on the inner wall of the outer shell, and the upper end of the outer wall of the grinding cylinder is rotatably connected to the fixed bearing; the plurality of annular sealing strips are located above the fixed bearing and are fixedly connected to the inner wall of the outer shell in sequence along the axial direction of the outer shell; the lower end of the sealing cover is provided with an annular sealing plate, and the annular sealing plate abuts against the annular sealing strip to complete the sealing.

[0020] The present invention has the further beneficial effect that the grinding cylinder is rotatably assembled in the housing by means of a fixed bearing, and the housing is sealed by cooperation of a plurality of sealing strips and a sealing ring of a sealing cover to prevent volatile nitrogen from escaping.

[0021] Furthermore, it also includes a support assembly, which includes a hollow base and a rotating shaft. The rotating shaft is arranged perpendicular to the axial direction of the shell and is rotatably installed on the base. The shell is located in the base, and the upper end of the side wall is rotatably connected to the rotating shaft.

[0022] A further beneficial effect of the present invention is that by providing a base and a rotating shaft, the outer shell connected to the grinding cylinder inside thereof can be swung along the rotating shaft, which can drive the frozen sample inside the grinding cylinder to repeatedly contact the grinding column inside the grinding cylinder, helping the grinding cylinder to quickly complete the initial crushing and grinding of the frozen sample.

[0023] Furthermore, it also includes a liquid nitrogen pump and a compressor. The liquid nitrogen pump is connected to the liquid nitrogen storage tank, and its air outlet is connected to the liquid nitrogen inlet; the air inlet of the compressor is connected to the liquid nitrogen outlet to recover nitrogen.

[0024] A further beneficial effect of the utility model is that the liquid nitrogen is gasified by the liquid nitrogen pump and then enters the device along the medium inlet, and then flows out from the medium outlet and is compressed and recovered by the compressor, thereby achieving a recycling effect and saving resources.

[0025] The device for freezing and grinding animal and plant samples provided by the present invention has the following significant improvements compared to the prior art:

[0026] 1. The nested structure can ensure that the liquid nitrogen can fully freeze the sample in the grinding cylinder quickly while preventing the liquid nitrogen from escaping;

[0027] 2. The rotating grinding cylinder and its internal grinding column structure replace the rotating shaft and blade structure, which extends the service life of the device;

[0028] 3. The additional rolling head can effectively ensure the quality of the final grinding. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is an overall schematic diagram of a device for freezing and grinding animal and plant samples provided by the utility model;

[0030] Figure 2 This is a front view of a device for freezing and grinding animal and plant samples provided by the utility model;

[0031] Figure 3 A top view of a device for freezing and grinding animal and plant samples provided by the utility model;

[0032] Figure 4 for Figure 2 sectional view of

[0033] Figure 5 for Figure 3 sectional view of

[0034] Figure 6 for Figure 4 A magnified schematic diagram of point A in the middle;

[0035] Figure 7 for Figure 4 Enlarged schematic diagram of point B in the middle.

[0036] Reference numerals

[0037] 1. Housing; 110. Liquid nitrogen inlet; 120. Liquid nitrogen outlet; 2. Liquid nitrogen storage tank; 3. Grinding assembly; 310. Grinding cylinder; 320. Grinding cylinder drive mechanism; 321. Rotating motor; 322. Rotating support seat; 330. Grinding column; 340. Electric cylinder; 350. Connecting rod; 360. Rolling head; 361. Bump; 370. Hollow limit column; 4. Sealing cover; 410. Annular sealing plate; 5. Guide ring plate; 510. Blade; 6. Fixed bearing; 7. Annular sealing strip; 8. Support assembly; 810. Base; 820. Rotating shaft. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] As shown in the figure, a freezing and grinding device for animal and plant samples includes a housing 1, a liquid nitrogen storage tank 2, a grinding assembly 3 and a sealing cover 4. The upper end of the housing 1 is open and can be sealed by the sealing cover 4. The lower end of the side wall thereof extends outwardly to connect with the liquid nitrogen inlet 110 and the liquid nitrogen outlet 120. The liquid nitrogen inlet 110 and the liquid nitrogen outlet 120 are arranged horizontally and opposite to each other; the liquid nitrogen storage tank 2 is connected to the liquid nitrogen inlet 110 through a delivery pipe to drive the liquid nitrogen from the liquid nitrogen inlet 110 into the housing 1 and then out of the liquid nitrogen outlet 120 to achieve rapid freezing; the grinding assembly 3 includes a grinding cylinder 310, a grinding cylinder drive mechanism 320 and a plurality of grinding columns 330 The diameter of the grinding cylinder 310 is smaller than that of the outer shell 1. Similarly, the upper end is open and coaxially located inside the outer shell 1. Its outer wall is rotatably connected to the inner wall of the outer shell 1. The grinding cylinder driving mechanism 320 is fixedly mounted on the outer wall of the outer shell 1, and its output end passes through the outer wall of the outer shell 1 and is transmission-connected to the grinding cylinder 310 to drive the grinding cylinder 310 to rotate inside the outer shell 1. Multiple grinding columns 330 are parallel to the axial direction of the grinding cylinder 310 and are arranged in sequence along the circumference of the inner wall of the grinding cylinder 310 and welded to the lower end of the side wall of the grinding cylinder 310. The lower end of the grinding column 330 is flush with the connection between the side wall and the bottom of the grinding cylinder 310. In this embodiment, the number of grinding columns 330 is 10.

[0040] Through the above technical scheme, the present invention provides a freezing and grinding device for animal and plant samples, which utilizes an independent and nested shell 1 and a grinding cylinder 310, in conjunction with a liquid nitrogen storage tank 2, so that the liquid nitrogen circulates in the chamber formed between the shell 1 and the grinding cylinder 310 to complete the freezing of the animal and plant samples in the grinding cylinder 310. The grinding cylinder 310 is rotatably connected to the shell 1 and is driven to rotate by a driving device. The grinding column 330 in its own cylinder can crush and grind the frozen sample under rotation. When the grinding cylinder 310 starts to rotate, the frozen sample is thrown toward the inner wall of the grinding cylinder 310 under the action of centrifugal force, and gradually crushed under the impact after contacting the grinding column 330. Compared with the grinding device in the prior art, the traditional rotating blade method is abolished and replaced with a grinding column 330 directly fixed on the inner wall of the grinding cylinder 310. There is no need to replace the blade or the rotating shaft and other structures. In addition, the presence of the sealing cover 4 can also effectively prevent the leakage of volatile nitrogen and the splashing of the ground powder out of the grinding cylinder 310.

[0041] To further improve the technical solution, the outer wall of each grinding column 330 has ridges along its circumference, and the number of ridges is an even number and at least 6, so that the cross-section of each grinding column 330 is a regular polygon, one side of the regular polygon is used to fit and weld with the inner wall of the grinding cylinder 310, and the remaining polygons and ridges are used to collide with the frozen sample rotating at high speed with the grinding cylinder 310. The presence of the ridges can make the frozen sample more concentrated when it contacts the grinding column 330, which helps to quickly break it into small pieces and then gradually into powder. In this embodiment, each grinding column 330 has six ridges and a regular hexagonal cross-section.

[0042] To further improve the technical solution, the grinding assembly 3 also includes an electric cylinder 340, a connecting rod 350 and a rolling head 360. The sealing cover 4 has an assembly cavity, and the electric cylinder 340 is installed in the assembly cavity. One end of the connecting rod 350 passes through the middle of the lower end of the sealing cover 4 and is transmission-connected to the movable end of the electric cylinder 340; the position of the connecting rod 350 ensures that it is in the same straight line as the axis of the grinding cylinder 310, and one end of the rolling head 360 is fixedly connected to the other end of the connecting rod 350, and the shape of the other end is adapted to the bottom of the grinding cylinder 310, and a plurality of spherical protrusions 361 are detachably connected to its surface. Through the above technical solution, the rolling head 360 can be used to perform preliminary crushing of the frozen sample, and the sample after preliminary crushing is convenient for further refinement by the grinding column 330. The spherical protrusions 361 on the rolling head 360 can increase the pressure of the rolling head 360 on the sample, so that the rolling head 360 can easily crush the frozen sample.

[0043] In order to further improve the technical solution, the grinding component 3 also includes a hollow limit column 370 made of an insulation board. One end of the hollow limit column 370 is vertically and fixedly connected to the lower end of the sealing cover 4. The outer wall of the connecting rod 350 is slidably connected to the inner wall of the hollow limit column 370. Through the above technical solution, the utility model can use the hollow limit column 370 to protect the connecting rod 350, and the existence of the hollow limit column 370 can play a certain guiding role to prevent the connecting rod 350 from being offset when it moves back and forth up and down under the drive of the electric cylinder 340 due to insufficient support and limitation on all sides, thereby affecting the effect of the initial crushing.

[0044] In order to further improve the technical solution, the bottom of the grinding cylinder 310 is arc-shaped, and the shape of the lower end surface of the rolling head 360 is adapted to the shape of the bottom of the grinding cylinder 310, and the multiple protrusions 361 are spirally distributed along the arc surface of the rolling head 360 with the fixed point of the arc surface of the rolling head 360 as the starting point. Through the above technical solution, the utility model can make the sample gather at the bottom of the grinding cylinder 310, and then cooperate with the rolling head 360 whose shape is adapted to the bottom of the grinding cylinder 310 to ensure that the actual effective rolling area is the same as the bottom area of ​​the grinding cylinder 310. The spirally distributed protrusions combined with the arc surface can also effectively ensure the uniformity of the rolling process.

[0045] To further improve the technical solution, the grinding cylinder drive mechanism 320 includes a rotating motor 321, a reducer and a rotating support seat 322. The casing of the rotating motor 321 is fixed to the bottom of the outer shell 1 by bolts; the reducer is coaxially installed on the motor shaft of the rotating motor 321, and the output shaft of the reducer passes vertically through the bottom of the outer shell 1 and is rotatably connected to the bottom of the outer shell 1. In order to ensure sealing, a sealing ring is installed at the connection between the output shaft of the reducer and the bottom of the outer shell 1. In particular, it is a metal seal. The lower end face of the rotating support seat 322 is rotatably connected to the bottom of the outer shell 1. In order to reduce friction, a ball connection can be used. At the same time, a mounting blind hole is opened at the bottom center of the rotating support seat 322. The output shaft of the reducer is inserted into the mounting blind hole and fixedly connected to it. The upper end face of the rotating support seat 322 adapts to the arc of the bottom of the grinding cylinder 310 and is coaxially fixed and welded to the grinding cylinder 310. Through the above technical solution, this application uses a rotating motor 321 and a reducer to realize reduction transmission, and drives the grinding cylinder 310 to rotate through the rotating support seat 322. Due to the supporting effect of the rotating support seat 322, the stability of the grinding cylinder 310 can be guaranteed after it starts to rotate.

[0046] In order to further improve the technical solution, it also includes multiple guide ring plates 5, which are coaxially fixed to the outer wall of the grinding cylinder 310 along the axial direction of the grinding cylinder 310. Each guide ring plate 5 is provided with multiple blades 510 at intervals, and the blade surfaces of the multiple blades 510 are arranged obliquely with respect to the horizontal direction. Through the above technical solution, the utility model installs multiple guide ring plates 5 on the outer wall of the grinding cylinder 310 so that the guide ring plates 5 rotate together with the grinding cylinder 310. The rotation of the guide ring plates 5 drives the airflow of the outer shell 1, thereby assisting the liquid nitrogen to quickly fill the space inside the outer shell 1.

[0047] To further improve the technical solution, the invention further includes a fixed bearing 6 and multiple annular sealing strips 7. The fixed bearing 6 is mounted on the inner wall of the housing 1, and the upper end of the outer wall of the grinding cylinder 310 is rotatably connected to the fixed bearing 6. The multiple annular sealing strips 7 are located above the fixed bearing 6 and are fixedly connected to the inner wall of the housing 1 in sequence along the axial direction of the housing 1. The lower end of the sealing cover 4 is provided with an annular sealing plate 410, which abuts against the annular sealing strips 7 to complete the seal. In particular, the annular sealing strips 7 are made of metal. Through the above technical solution, the present invention utilizes the fixed bearing 6 to rotatably assemble the grinding cylinder 310 within the housing 1, and utilizes the multiple annular sealing strips 7 and the annular sealing plate 410 at the lower end of the sealing cover 4 to complete the sealing of the housing 1, preventing the escape of volatile nitrogen.

[0048] In order to further improve the technical solution, it also includes a support component 8, which includes a hollow base 810 and a rotating shaft 820. The base 810 is a rectangular hollow shell, and there are two rotating shafts 820, which are arranged opposite to each other and horizontally rotated on two opposite sides of the base 810. The outer shell 1 is arranged in the base 810, and the upper ends of the side walls are rotatably connected to the two rotating shafts 820, and the outer bottom is suspended. Through the above technical solution, the outer shell 1 together with the grinding cylinder 310 inside it can be swung along the rotating shaft 820, which can drive the frozen sample inside the grinding cylinder 310 to repeatedly contact the grinding column 330 inside the grinding cylinder 310, helping the grinding cylinder 310 to quickly complete the crushing and grinding of the frozen sample.

[0049] In order to further improve the technical solution, a liquid nitrogen pump and a compressor 9 are also provided. The liquid nitrogen pump is directly connected to the liquid nitrogen storage tank 2, and its gas outlet is connected to the liquid nitrogen inlet 110 through a delivery pipe. The liquid nitrogen pump is a prior art and is a conventional practical tool for quickly gasifying liquid nitrogen in the field of liquid nitrogen applications. Since the principle and usage method are common knowledge in the field, they will not be described here and are not shown in the figure. The compressor 9 is connected to the liquid nitrogen outlet 120. The compressor 9 is used to collect the nitrogen leaving the device and re-compress it into liquid nitrogen. The compressor 9 can be connected to an empty liquid nitrogen storage tank 2 or directly form a liquid nitrogen circulation device with the current liquid nitrogen storage tank 2. Since the principle and usage method of the compressor are also common knowledge in the field of heat exchange technology, they will not be described here. Through the above technical solution, the utility model can use the liquid nitrogen pump to quickly gasify the liquid nitrogen and then pass it into the interior of the housing 1 along the liquid nitrogen inlet 110, and under the action of the annular guide plate 5, help the gasified liquid nitrogen to flow out through the liquid nitrogen outlet 120 and be recompressed by the compressor 9, thereby realizing the recycling of liquid nitrogen and achieving the effect of saving resources.

[0050] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and purpose of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A freezing and grinding device for animal and plant samples, characterized in that: The invention comprises a housing (1), a liquid nitrogen storage tank (2), a grinding assembly (3) and a sealing cover (4); the upper end of the housing (1) is open, and the side walls thereof are respectively provided with a liquid nitrogen inlet (110) and a liquid nitrogen outlet (120); the outlet end of the liquid nitrogen storage tank (2) is connected to the liquid nitrogen inlet (110); The grinding assembly (3) comprises a grinding cylinder (310), a grinding cylinder driving mechanism (320) and a plurality of grinding posts (330); the upper end of the grinding cylinder (310) is open and located inside the housing (1); the outer wall of the grinding cylinder is rotatably connected to the inner wall of the housing (1); the grinding cylinder driving mechanism (320) is fixedly mounted on the outer wall of the housing (1); and the output end thereof passes through the outer wall of the housing (1) and is transmission-connected to the grinding cylinder (310); the plurality of grinding posts (330) are parallel to the axial direction of the grinding cylinder (310) and are fixedly connected to the inner wall of the grinding cylinder (310) in sequence along the circumferential direction of the inner wall of the grinding cylinder (310); The sealing cover (4) is detachably connected to the upper end of the housing (1).

2. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The outer wall of each grinding column (330) has a plurality of ridges along its circumference, and the number of the ridges is an even number and is at least six.

3. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The grinding assembly (3) further comprises an electric cylinder (340), a connecting rod (350) and a rolling head (360); the sealing cover (4) has an assembly cavity therein, and the electric cylinder (340) is installed in the assembly cavity. One end of the connecting rod (350) passes through the lower end of the sealing cover (4) and is transmission-connected to the movable end of the electric cylinder (340); one end of the rolling head (360) is fixedly connected to the other end of the connecting rod (350), and the shape of the other end is adapted to the bottom of the grinding cylinder (310), and a plurality of spherical protrusions (361) are detachably connected to its surface, and the electric cylinder (340) drives the protrusions (361) to abut against the bottom of the grinding cylinder (310).

4. The freezing and grinding device for animal and plant samples according to claim 3, characterized in that: The grinding assembly (3) further comprises a hollow limiting column (370) made of a thermal insulation plate, one end of the hollow limiting column (370) being vertically and fixedly connected to the lower end of the sealing cover (4), and the outer wall of the connecting rod (350) being slidably connected to the inner wall of the hollow limiting column (370).

5. The freezing and grinding device for animal and plant samples according to claim 4, characterized in that: The bottom of the grinding cylinder (310) is arc-shaped, and the plurality of protrusions (361) start from the lower end of the rolling head (360) and are distributed in a spiral shape on the surface of the rolling head (360).

6. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The grinding cylinder driving mechanism (320) comprises a rotating motor (321), a speed reducer and a rotating support seat (322), wherein the housing of the rotating motor (321) is fixedly mounted on the bottom of the housing (1); The reducer is coaxially mounted on the motor pump of the rotating motor (321); the output shaft of the reducer vertically passes through the bottom of the housing (1) and the shaft body is provided with a sealing ring, and is rotatably connected to the bottom of the housing (1); the lower end surface of the rotating support seat (322) is rotatably connected to the inner bottom of the housing (1) and fixedly connected to the output shaft of the reducer; the upper end surface of the rotating support seat (322) is fixedly connected to the outer bottom of the grinding cylinder (310).

7. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The invention also comprises a plurality of guide ring plates (5), which are coaxially fixed to the outer wall of the grinding cylinder (310) in sequence along the axial direction of the grinding cylinder (310), and each of the guide ring plates (5) is provided with a plurality of blades (510) for assisting nitrogen gas flow guidance at intervals.

8. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The invention also includes a fixed bearing (6) and a plurality of annular sealing strips (7), wherein the fixed bearing (6) is mounted on the inner wall of the outer shell (1), and the upper end of the outer wall of the grinding cylinder (310) is rotatably connected to the fixed bearing (6); the plurality of annular sealing strips (7) are located above the fixed bearing (6) and are fixedly connected to the inner wall of the outer shell (1) in sequence along the axial direction of the outer shell (1); and an annular sealing plate (410) is provided at the lower end of the sealing cover (4), and the annular sealing plate (410) is in sealing contact with the annular sealing strip (7).

9. The freezing and grinding device for animal and plant samples according to claim 1, characterized in that: The invention also includes a support assembly (8), wherein the support assembly (8) includes a hollow base (810) and a rotating shaft (820), wherein the rotating shaft (820) is arranged horizontally and is rotatably mounted on the side wall of the base (810), and the housing (1) is located in the base (810), and the upper end of the side wall thereof is perpendicular to and fixedly connected to the rotating shaft (820).

10. The freezing and grinding device for animal and plant samples according to any one of claims 1 to 9, characterized in that: It also includes a liquid nitrogen pump and a compressor (9), wherein the liquid nitrogen pump is connected to the liquid nitrogen storage tank (2), and its gas outlet is connected to the liquid nitrogen inlet (110); the gas inlet of the compressor (9) is connected to the liquid nitrogen outlet (120) to recover nitrogen.

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