Medium changing device

Abstract

The utility model relates to the technical field of cell culture, and discloses a culture medium liquid changing device which comprises a bottom plate and a pipette, a positioning block and a telescopic rod are fixedly installed at the upper end of the bottom plate, a first spring is installed on the outer side of the telescopic rod in a sleeved mode, and a fixed rail is fixedly installed at the upper end of the telescopic rod. A sliding sleeve is movably installed on the outer side of the fixed rail, an adsorption block is fixedly installed at the upper end of the fixed rail, and a magnetic adsorption block is fixedly installed in the sliding sleeve. Through mutual cooperation of the telescopic rod, the spring I, the fixed rail, the sliding sleeve, the adsorption block and the magnetic adsorption block, when the pipette moves up and down and horizontally, an auxiliary guiding effect is achieved, and pollution caused by frequent contact between a pipette and the inner wall of a culture plate in a liquid changing process can be avoided; through mutual cooperation of the fixed sleeve, the movable frame, the second spring and the push plate, a plurality of pipettes can be taken down from the suction head at the same time and do not need to be manually removed one by one, and the replacement convenience of the pipettes is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a culture medium replacement device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. The substances required for cell growth are mainly supplied by the culture medium, and staff need to replace the culture medium regularly.

[0003] According to the search, Chinese patent document, publication number: CN210001852U, discloses a culture medium replacement device, including a pipette, a support portion and a limit portion; the pipette includes a pipette branch and a pipette main pipette, and the support portion can fix multiple pipette branches in the holes of the external culture plate / dish, and then the culture liquid in the hole of the external culture plate / dish is sucked into the pipette main pipette through the multiple pipette branches, and due to the effect of the limit portion, it can be ensured that the pipette branches will not move freely or tilt when entering the hole of the external culture plate / dish, thereby ensuring the stability of the pipetting process, alleviating the low efficiency, easy error and contamination of manual liquid replacement in the prior art, and the high experimental cost of liquid replacement in the workstation, saving the manual operation cost during the culture medium pipetting process, and being more practical. However, when the culture medium replacement device is in use, due to the small opening of the culture plate, the pipette branch may be contaminated due to frequent contact with the inner wall due to the hand trembling of the staff, and it does not have an auxiliary guiding function, and the device cannot help the staff align with the opening of the culture plate. Utility Model Content

[0004] (1) Technical problems solved

[0005] In view of the deficiencies in the prior art, the present invention provides a culture medium replacement device, which can solve the problems raised in the above background technology.

[0006] (2) Technical solution

[0007] The top end of the lifting link is connected with the lifting link of the lifting link, and the bottom end of the lifting link is connected with the spring, the spring being fixedly mounted on the lifting link of the lifting link.

[0008] Preferably, four positioning blocks and four telescopic rods are provided respectively, and the upper end of the first spring is in contact with the movable end of the telescopic rod.

[0009] Through the above technical solution, the spring pair applies an upward elastic force to the telescopic rod, which plays a resetting role after the telescopic rod moves downward.

[0010] Preferably, two fixed rails are symmetrically arranged, a plurality of adsorption blocks are equidistantly arranged, and the magnetic blocks are magnetically connected to the adsorption blocks.

[0011] Through the above technical solution, the sliding sleeve moves horizontally along the outer side of the fixed rail, and the magnetic block is adsorbed together with the adsorption block through magnetic force, which facilitates the positioning of the pipette and prevents the position of the pipette from shifting during the liquid change process.

[0012] Preferably, two positioning rods are symmetrically provided, and the positioning rods are sleeved and installed inside the positioning sleeve.

[0013] Through the above technical solution, the positioning rod is sleeved inside the positioning sleeve from top to bottom, thereby supporting and positioning the pipette.

[0014] Preferably, a plurality of the suction heads are arranged at equal intervals, and the pipettes correspond to the suction heads one by one.

[0015] Through the above technical solution, the pipette adsorbs the culture medium through the suction tip and the pipette.

[0016] Preferably, four fixed sleeves are symmetrically provided, and two movable frames are provided.

[0017] Through the above technical solution, the fixed sleeve plays a guiding role on the movable frame.

[0018] Preferably, the lower end of the second spring is fixedly connected to the pipette gun, and the lower end of the push plate is fitted with the upper end of the connecting plate.

[0019] Through the above technical solution, the movable frame is pressed downward, the spring 2 is compressed, and the push plate removes the pipette from the suction head through the connecting plate. There is no need to remove them one by one manually, which facilitates the replacement of the pipette. After the movable frame is released, the spring 2 recovers and applies an upward elastic force to the movable frame to complete the reset.

[0020] Compared with the prior art, the present invention provides a culture medium replacement device with the following beneficial effects:

[0021] 1. The telescopic rod, spring 1, fixed rail, sliding sleeve, adsorption block and magnetic block provided in the utility model cooperate with each other, which plays an auxiliary guiding role when the pipette moves up and down and horizontally, and can avoid contamination caused by frequent contact between the pipette and the inner wall of the culture plate during liquid exchange.

[0022] 2. The utility model can remove multiple pipettes from the suction head at the same time by cooperating with the fixed sleeve, movable frame, spring 2 and push plate, without the need to remove them manually one by one, thereby improving the convenience of pipette replacement. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the three-dimensional structure of the utility model;

[0024] Figure 2 This is a schematic diagram of the three-dimensional structure of the bottom plate of the utility model;

[0025] Figure 3 This is a schematic diagram of the three-dimensional structure of the pipette gun of the utility model Figure 1 ;

[0026] Figure 4 This is a schematic diagram of the three-dimensional structure of the pipette gun of the utility model Figure 2 ;

[0027] Figure 5 This is a schematic diagram of the three-dimensional structure of the pipette of the utility model;

[0028] Figure 6 It is a schematic diagram of the three-dimensional structure of the movable frame of the utility model.

[0029] Among them: 1. Base plate; 2. Positioning block; 3. Telescopic rod; 4. Spring 1; 5. Fixed rail; 6. Sliding sleeve; 7. Adsorption block; 8. Magnetic block; 9. Positioning sleeve; 10. Pipette; 11. Positioning rod; 12. Tip; 13. Pipette; 14. Connecting plate; 15. Fixed sleeve; 16. Movable frame; 17. Spring 2; 18. Push plate. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] Example 1:

[0032] like Figure 1-6 As shown, the utility model provides a culture medium liquid changing device, including a base plate 1 and a pipette gun 10, the upper end of the base plate 1 is fixedly installed with a positioning block 2 and a telescopic rod 3, the outer side of the telescopic rod 3 is sleeved with a spring 1 4, the upper end of the telescopic rod 3 is fixedly installed with a fixed rail 5, the outer side of the fixed rail 5 is movably installed with a sliding sleeve 6, the upper end of the fixed rail 5 is fixedly installed with an adsorption block 7, the interior of the sliding sleeve 6 is fixedly installed with a magnetic block 8, the outer side of the sliding sleeve 6 is fixedly installed with a positioning sleeve 9, the outer side of the pipette gun 10 is fixedly installed with a positioning rod 11, the lower end of the pipette gun 10 is fixedly installed with a suction head 12, the outer side of the suction head 12 is sleeved with a pipette 13, the outer side of the opening of the pipette 13 is fixedly installed with a connecting plate 14, the outer side of the pipette gun 10 is fixedly installed with a fixed sleeve 15, the inner side of the fixed sleeve 15 is movably installed with a movable frame 16, the inner side of the movable frame 16 is fixedly installed with a spring 2 17, and the lower end of the movable frame 16 is fixedly installed with a push plate 18.

[0033] Specifically, the positioning block 2 and the telescopic rod 3 are respectively provided with four, and the upper end of the spring 1 4 is in contact with the movable end of the telescopic rod 3. The advantage is that the spring 1 4 applies an upward elastic force to the telescopic rod 3, which plays a reset role after it moves downward.

[0034] Specifically, two fixed rails 5 are symmetrically arranged, multiple adsorption blocks 7 are equidistantly arranged, and the magnetic blocks 8 are magnetically connected to the adsorption blocks 7. This has the advantage that when the sleeve 6 moves horizontally along the outside of the fixed rail 5, the magnetic blocks 8 are magnetically attached to the adsorption blocks 7, making it easier to position the pipette 10 and preventing the pipette 10 from shifting during liquid exchange.

[0035] Specifically, two positioning rods 11 are symmetrically provided, and the positioning rods 11 are sleeved and installed inside the positioning sleeve 9. The advantage is that the positioning rods 11 are sleeved inside the positioning sleeve 9 from top to bottom, and play a supporting and positioning role for the pipette gun 10.

[0036] Example 2:

[0037] like Figure 1-6As shown, as an improvement to the previous embodiment, specifically, a plurality of suction tips 12 are arranged at equal intervals, and the pipettes 13 correspond one to one with the suction tips 12. The advantage is that the pipette gun 10 absorbs the culture medium through the suction tips 12 and the pipettes 13.

[0038] Specifically, four fixed sleeves 15 are symmetrically provided, and two movable frames 16 are provided. The advantage is that the fixed sleeves 15 play a guiding role for the movable frames 16.

[0039] Specifically, the lower end of the second spring 17 is fixedly connected to the pipette gun 10, and the lower end of the push plate 18 is in contact with the upper end of the connecting plate 14. Advantageously, by pressing the movable frame 16 downward, the second spring 17 is compressed, and the push plate 18 removes the pipette 13 from the tip 12 via the connecting plate 14, eliminating the need to manually remove each pipette 13 one by one, making it easier to replace the pipette 13. After releasing the movable frame 16, the second spring 17 recovers, exerting an upward elastic force on the movable frame 16 to complete the reset.

[0040] When in use, place the culture plate that needs to be changed on the bottom plate 1 and align it with the positioning block 2 to prevent it from moving. Put a clean pipette 13 on the outside of the suction head 12, and connect the multiple pipettes 13 together with the connecting plate 14 for easy installation at the same time. The positioning rod 11 is put on the inside of the positioning sleeve 9 from top to bottom to support and position the pipette gun 10. Move the pipette gun 10 horizontally, and the sliding sleeves 6 on both sides move horizontally along the outside of the fixed rail 5 to guide the pipette gun 10. When it moves to the position where the liquid needs to be changed, the magnetic block 8 is adsorbed together with the adsorption block 7 by magnetic force, which is convenient for positioning the pipette gun 10 and prevents the position of the pipette gun 10 from shifting during the liquid change process. Press the pipette gun 10 downward, the telescopic rod 3 contracts, the spring 14 is compressed, and the pipette 13 extends into the culture plate to adjust the culture medium. After the absorption is completed, the pipette gun 10 is removed and the culture medium is discharged into the waste liquid tank. The movable frame 16 is pressed downward, the spring 2 17 is compressed, and the push plate 18 removes the pipette 13 from the suction head 12 through the connecting plate 14. There is no need to manually remove them one by one, which is convenient for replacing the pipette 13. After loosening the movable frame 16, the spring 2 17 is restored, and an upward elastic force is applied to the movable frame 16 to complete the reset. Then a new pipette 13 is replaced. After the new culture medium is absorbed, the positioning rod 11 is again put into the positioning sleeve 9, and the culture medium is squeezed into the culture plate to realize the liquid exchange operation. The device plays an auxiliary positioning function during the liquid exchange process, which can effectively avoid the frequent contact of the pipette 13 with the inner wall of the culture plate caused by hand shaking during the liquid exchange process to cause contamination. It is suitable for the simultaneous liquid exchange operation of multiple culture media.

[0041] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A culture medium replacement device, comprising a base plate (1) and a pipette (10), characterized in that: The upper end of the base plate (1) is fixedly mounted with a positioning block (2) and a telescopic rod (3), the outer side of the telescopic rod (3) is sleeved with a spring (4), the upper end of the telescopic rod (3) is fixedly mounted with a fixed rail (5), the outer side of the fixed rail (5) is movably mounted with a sliding sleeve (6), the upper end of the fixed rail (5) is fixedly mounted with an adsorption block (7), the interior of the sliding sleeve (6) is fixedly mounted with a magnetic adsorption block (8), the outer side of the sliding sleeve (6) is fixedly mounted with a positioning sleeve (9), the outer side of the pipette gun (10) is fixedly mounted with a fixed A positioning rod (11) is provided, and a suction head (12) is fixedly installed at the lower end of the pipette gun (10), a pipette (13) is sleeved on the outer side of the suction head (12), a connecting plate (14) is fixedly installed on the outer side of the opening of the pipette (13), a fixed sleeve (15) is fixedly installed on the outer side of the pipette gun (10), a movable frame (16) is movably installed on the inner side of the fixed sleeve (15), a spring 2 (17) is fixedly installed on the inner side of the movable frame (16), and a push plate (18) is fixedly installed on the lower end of the movable frame (16).

2. A culture medium replacement device according to claim 1, characterized in that: The positioning blocks (2) and the telescopic rods (3) are respectively provided in four numbers, and the upper end of the spring 1 (4) is fitted with the movable end of the telescopic rod (3).

3. A culture medium replacement device according to claim 1, characterized in that: Two fixed rails (5) are symmetrically arranged, a plurality of adsorption blocks (7) are equidistantly arranged, and the magnetic block (8) is magnetically connected to the adsorption block (7).

4. A culture medium replacement device according to claim 1, characterized in that: Two positioning rods (11) are symmetrically arranged, and the positioning rods (11) are sleeved and installed inside the positioning sleeve (9).

5. A culture medium replacement device according to claim 1, characterized in that: A plurality of suction tips (12) are arranged at equal intervals, and the pipettes (13) correspond one to one to the suction tips (12).

6. A culture medium replacement device according to claim 1, characterized in that: Four fixed sleeves (15) are symmetrically provided, and two movable frames (16) are provided.

7. A culture medium replacement device according to claim 1, characterized in that: The lower end of the second spring (17) is fixedly connected to the pipette gun (10), and the lower end of the push plate (18) is in contact with the upper end of the connecting plate (14).

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