Novel real-time fluorescent quantitative PCR instrument

Abstract

The utility model discloses a novel real-time fluorescent quantitative PCR instrument, which relates to the technical field of PCR instruments and comprises an instrument main body and a sealing cover which are matched with each other, sealing seats are arranged on two sides of the instrument main body, sealing grooves are arranged on the surfaces of the sealing seats, and a sampling detection component is fixedly connected inside the instrument main body. The sampling detection assembly comprises a detection box main body and a base which are fixedly connected up and down, a placement cavity channel is formed in the top of the detection box main body, and a limiting cavity channel is formed in the surface of the placement cavity channel. According to the design, the effect of supporting and placing an external sampling test tube is achieved by matching the trapezoidal concave placement cavity with the limiting cavity formed in the surface, and the heat preservation effect is enhanced when the external sampling test tube is heated through the heat preservation layer.

Description

Technical Field

[0001] The utility model relates to the field of PCR instruments, in particular to a novel real-time fluorescence quantitative PCR instrument. Background Art

[0002] A PCR amplifier, also known as a PCR gene amplifier, PCR nucleic acid amplifier, or polymerase chain reaction nucleic acid amplifier, is an instrument that uses PCR technology to amplify specific DNA. It is widely used in medical and biological laboratories, for example, to determine whether a specimen will exhibit a pattern of a genetic disease, diagnose infectious diseases, replicate genes, and perform paternity testing. A PCR instrument that adds a fluorescence signal acquisition system and a computer analysis and processing system to a conventional PCR instrument is called a fluorescence quantitative PCR instrument. Its PCR amplification principle is the same as that of a conventional PCR instrument, except that the primers added during PCR amplification are labeled with isotopes, fluorescein, etc., and the primers and fluorescent probes are used to simultaneously bind specifically to the template for amplification. The amplification results are collected in real time by the fluorescence signal acquisition system and transmitted to a computer analysis and processing system for quantitative real-time output. This type of PCR instrument is called a real-time fluorescence quantitative PCR instrument.

[0003] Fluorescence quantitative PCR instruments are available in single, dual, and multichannel formats. A single channel is recommended when labeling with a single fluorescent probe, while a multichannel format is recommended when multiple fluorescent probes are used. A single channel can also detect target gene expression products with multiple fluorescent markers, but since only the amplification of one target gene can be detected at a time, multiple amplifications are required to detect the abundance of different target gene fragments. These instruments are primarily used in medical clinical testing, biopharmaceutical R&D, the food industry, and research institutions.

[0004] The current real-time fluorescence quantitative PCR instrument is unable to perform convenient sampling and heating control on the objects to be detected, resulting in low detection efficiency.

[0005] Therefore, the present invention proposes a novel real-time fluorescence quantitative PCR instrument. Utility Model Content

[0006] In view of the deficiencies in the prior art, the present invention provides a novel real-time fluorescence quantitative PCR instrument, which solves the problems raised by the above-mentioned background technology.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a new real-time fluorescence quantitative PCR instrument, including a matching instrument body and a sealing cover, sealing seats are provided on both sides of the instrument body, and a sealing groove is provided on the surface of the sealing seat. A sampling and detection component is fixedly connected to the inside of the instrument body, and the sampling and detection component includes a detection box body and a base fixedly connected in an upper and lower position. A placement cavity is provided on the top of the detection box body, a limited position cavity is provided on the surface of the placement cavity, and an insulation layer is provided on the surface of the placement cavity. The outer end of the detection box body is fixedly connected to a board, and a heating chamber and a heating chamber are provided inside the base. A heating tube is fixedly connected to the inside of the heating chamber, and a pipeline is fixedly connected to the surface of the base.

[0008] As a further technical solution of the present invention, the sealing seat is located on both sides of the top of the instrument body, and is arranged longitudinally in a trapezoidal shape. It is set as a plastic sealing seat, and the sealing groove is opened longitudinally in a trapezoidal shape along the surface of the sealing seat.

[0009] As a further technical solution of the present invention, longitudinally protruding sealing protrusions are provided on both side surfaces of the sealing cover according to the sealing grooves.

[0010] As a further technical solution of the present invention, the main body of the detection box is arranged in a rectangular box body as a whole, and its interior is hollow. At the same time, the main body of the detection box and the heating chamber are vertically connected, and the straps are arranged in a trapezoidal strip shape, which are arranged longitudinally along the two ends of the main body of the detection box. The straps are also located on the inclined surface inside the sealing seat.

[0011] As a further technical solution of the present invention, the placement cavity is arranged in a trapezoidal concave shape along both sides of the top of the detection box body, multiple groups of limiting cavities are opened at equal distances along the surface of the placement cavity, and the insulation layer is arranged on the inclined surfaces on both sides of the placement cavity.

[0012] As a further technical solution of the present invention, the thermal insulation layer is configured as a glass wool thermal insulation layer.

[0013] As a further technical solution of the present invention, the bottom end of the base is arranged in an arc-shaped outward protrusion, the heating chamber and the heating chamber are arranged in an upper and lower position inside the base, and a group of copper heat-conducting partitions are arranged between the two, and the heating chamber is set as a water bath heating chamber.

[0014] As a further technical solution of the present invention, the heating tubes are configured as electric heating tubes, which are arranged in multiple groups in an "S" shape horizontally in the heating chamber and are configured as stainless steel 321 heating tubes.

[0015] The present invention provides a novel real-time fluorescence quantitative PCR instrument, which has the following advantages compared with the prior art:

[0016] 1. This design of a new real-time fluorescence quantitative PCR instrument achieves the effect of enhancing the sealing of the sealing cover to the top of the instrument body through the sealing groove on the surface of the sealing seat, supports the external sampling tube through the detection box body, and supports the entire sampling and detection assembly inside the instrument body through the bridge plate.

[0017] 2. A new type of real-time fluorescence quantitative PCR instrument designed in this paper achieves the effect of supporting and placing external sampling tubes through a placement cavity with a trapezoidal concave setting and a limiting cavity opened on the surface, and enhances the insulation effect when heating the external sampling tubes through the insulation layer.

[0018] 3. A new type of real-time fluorescence quantitative PCR instrument designed in this paper achieves the effect of heating the external sampling tube in a water bath through the heating chamber, and achieves the effect of heating the water source in the heating chamber through the heating tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of a new real-time fluorescence quantitative PCR instrument;

[0020] Figure 2 A new type of real-time fluorescence quantitative PCR instrument Figure 1 A magnified view of the structure at center A;

[0021] Figure 3 This is a schematic diagram of the connection of the sampling and detection components of a new real-time fluorescence quantitative PCR instrument;

[0022] Figure 4 A new type of real-time fluorescence quantitative PCR instrument Figure 3 Enlarged view of the structure at point B in the middle.

[0023] In the figure: 1. Instrument body; 2. Sealing cover; 3. Sampling and detection assembly; 4. Sealing seat; 5. Sealing groove; 6. Detection box body; 7. Placement cavity; 8. Laying board; 9. Insulation layer; 10. Base; 11. Heating chamber; 12. Heating chamber; 13. Pipeline; 14. Heating tube; 15. Limit cavity. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0025] See also Figure 1-4The utility model provides a new technical solution of real-time fluorescence quantitative PCR instrument: it includes an instrument body 1 and a sealing cover 2 that are arranged in a matching manner, sealing seats 4 are provided on both sides of the instrument body 1, and a sealing groove 5 is provided on the surface of the sealing seat 4. A sampling and detection component 3 is fixedly connected to the inside of the instrument body 1, and the sampling and detection component 3 includes a detection box body 6 and a base 10 that are fixedly connected in an upper and lower position. A placement cavity 7 is provided on the top of the detection box body 6, and a limited cavity 15 is provided on the surface of the placement cavity 7. An insulation layer 9 is provided on the surface of the placement cavity 7. The outer end of the detection box body 6 is fixedly connected to a strap 8, and a heating chamber 11 and a heating chamber 12 are provided inside the base 10. A heating tube 14 is fixedly connected to the inside of the heating chamber 12, and a pipe 13 is fixedly connected to the surface of the base 10.

[0026] like Figure 1-2 As shown, the sealing seat 4 is located as a whole on both sides of the top of the instrument body 1, and is arranged longitudinally in a trapezoidal shape. It is set as a plastic sealing seat, and the sealing groove 5 is opened longitudinally in a trapezoidal shape along the surface of the sealing seat 4. The two side surfaces of the sealing cover 2 are provided with longitudinally protruding sealing protrusions according to the sealing groove 5. The sealing groove 5 on the surface of the sealing seat 4 cooperates with the effect of enhancing the sealing performance of the sealing cover 2 on the top of the instrument body 1.

[0027] The detection box body 6 is arranged as a whole in a rectangular box body, and its interior is hollow. At the same time, the detection box body 6 and the heating chamber 11 are vertically connected. The strap 8 is arranged in a trapezoidal strip shape, which is arranged longitudinally along the two ends of the detection box body 6. The strap 8 is also located on the inner inclined surface of the sealing seat 4. The detection box body 6 is used to support the external sampling test tube, and the strap 8 is used to support the entire sampling detection component 3 inside the instrument body 1.

[0028] like Figure 3-4 As shown, the placement cavity 7 is set in a trapezoidal concave manner along both sides of the top of the detection box body 6, and multiple groups of limiting cavities 15 are opened at equal distances along the surface of the placement cavity 7. The insulation layer 9 is set on the inclined surfaces on both sides of the placement cavity 7. The insulation layer 9 is set as a glass wool insulation layer. The placement cavity 7 set in a trapezoidal concave manner is combined with the limiting cavity 15 opened on the surface to achieve the effect of supporting and placing the external sampling tube, and the insulation layer 9 is used to enhance the insulation effect when the external sampling tube is heated.

[0029] When placing an external sampling test tube, it is placed in the limiting cavity 15, and the top of the test tube is located in the heating chamber 11 for heating operation, and the raised position of the top of the test tube is limited to the inclined positions on both sides of the placement cavity 7 with a trapezoidal concave setting. During the heating process, the insulation layer 9 can effectively prevent the heat generated by the heating from being lost to the outside, thereby ensuring the heating effect of the sampling test tube.

[0030] The bottom end of the base 10 is arranged in an arc-shaped outward protrusion. The heating chamber 11 and the heating chamber 12 are distributed in the upper and lower positions inside the base 10, and a group of copper heat-conducting partitions are arranged between the two. The heating chamber 11 is set as a water bath heating chamber, and the heating tube 14 is set as an electric heating tube. It is arranged in multiple groups in an "S" shape horizontally in the heating chamber 12 and is set as a stainless steel 321 heating tube. The heating chamber 11 is used to heat the external sampling test tube in a water bath, and the heating tube 14 is used to heat the water source in the heating chamber 11.

[0031] A heated water source is added to the heating chamber 11 through the pipe 13, and then the sampling tube is placed in the sampling detection assembly 3. When the heating operation is performed, the heating tube 14 is started, and the heat is conducted through the copper heat-conducting partition between the heating chamber 11 and the heating chamber 12 to heat the water source in the heating chamber 11, thereby completing the heating operation of the sampling tube.

[0032] The working principle of the present invention is as follows: when the device is in use, the external sampling tube can be effectively and evenly heated by a water bath. By placing the cavity 7 in conjunction with the limiting cavity 15, a good heat preservation and sealing effect is achieved while limiting the sampling tube, thereby ensuring detection efficiency.

[0033] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications should be considered within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described or explained in this invention shall, unless otherwise specified or limited, be implemented in accordance with conventional means in the art.

Claims

1. A novel real-time fluorescence quantitative PCR instrument, characterized in that: The invention comprises an instrument body (1) and a sealing cover (2) which are arranged in a matched manner. Sealing seats (4) are arranged on both sides of the instrument body (1). Sealing grooves (5) are provided on the surface of the sealing seats (4). A sampling and detection component (3) is fixedly connected to the interior of the instrument body (1). The sampling and detection component (3) comprises a detection box body (6) and a base (10) which are fixedly connected in an upper and lower position. A placement cavity (7) is provided on the top of the detection box body (6), a limited cavity (15) is provided on the surface of the placement cavity (7), a heat-insulating layer (9) is provided on the surface of the placement cavity (7), an outer end of the detection box body (6) is fixedly connected to a strap (8), a heating chamber (11) and a heating chamber (12) are provided inside the base (10), a heating tube (14) is fixedly connected inside the heating chamber (12), and a pipe (13) is fixedly connected to the surface of the base (10).

2. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The sealing seat (4) is located on both sides of the top of the instrument body (1) as a whole, is arranged longitudinally in a trapezoidal shape, and is configured as a plastic sealing seat. The sealing groove (5) is longitudinally opened in a trapezoidal shape along the surface of the sealing seat (4).

3. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The two side surfaces of the sealing cover (2) are provided with longitudinally protruding sealing protrusions according to the sealing groove (5).

4. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The detection box body (6) is configured as a rectangular box body with a hollow interior. The detection box body (6) and the heating chamber (11) are vertically connected. The straps (8) are configured as trapezoidal strips and are longitudinally arranged along both ends of the detection box body (6). The straps (8) are also located on the inner inclined surface of the sealing seat (4).

5. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The placement cavity (7) is arranged in a trapezoidal concave shape along both sides of the top of the detection box body (6), and a plurality of limiting cavities (15) are opened at equal intervals along the surface of the placement cavity (7), and the insulation layer (9) is arranged on the inclined surfaces on both sides of the placement cavity (7).

6. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The thermal insulation layer (9) is configured as a glass wool thermal insulation layer.

7. A novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The bottom end of the base (10) is arranged in an arc-shaped outward protrusion, and the heating chamber (11) and the heating chamber (12) are arranged in an upper and lower position inside the base (10), and a group of copper heat-conducting partitions are arranged between the two. The heating chamber (11) is set as a water bath heating chamber.

8. The novel real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: The heating tubes (14) are configured as electric heating tubes, which are arranged in a plurality of groups in an "S" shape transversely in the heating chamber (12) and are configured as stainless steel 321 heating tubes.

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